TY  - JOUR
AU  - Vasiljevic, Zorka
AU  - Vunduk, Jovana
AU  - Bartolić, Dragana
AU  - Mišković, Goran
AU  - Ognjanović, Miloš
AU  - Tadić, Nenad B.
AU  - Nikolić, Maria Vesna
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13189
AB  - Emission of greenhouse gases and infectious diseases caused by improper agro-waste disposal has gained significant attention in recent years. To overcome these hurdles, agro-waste can be valorized into valuable bioactive compounds that act as reducing or stabilizing agents in the synthesis of nanomaterials. Herein, we report a simple circular approach using Citrus reticulata Blanco (C. reticulata) waste (peel powder/aqueous extract) as green reducing and capping/stabilizing agents and Zn nitrate/acetate precursors to synthesize ZnO nanoparticles (NPs) with efficient antimicrobial and photocatalytic activities. The obtained NPs crystallized in a hexagonal wurtzite structure and differed clearly in their morphology. UV-vis analysis of the nanoparticles showed a characteristic broad absorption band between 330 and 414 nm belonging to ZnO NPs. Fourier transform infrared (FTIR) spectroscopy of ZnO NPs exhibited a Zn-O band close to 450 cm-1. The band gap values were in the range of 2.84-3.14 eV depending on the precursor and agent used. The crystallite size obtained from size-strain plots from measured XRD patterns was between 7 and 26 nm, with strain between 16 and 4%. The highly crystalline nature of obtained ZnO NPs was confirmed by clear ring diffraction patterns and d-spacing values of the observed lattice fringes. ZnNPeelMan_400 and ZnNExtrMan showed good stability, as the zeta potential was found to be around −20 mV, and reduced particle aggregation. Photoluminescence analysis revealed different defects belonging to oxygen vacancies (VO+ and VO+2) and zinc interstitial (Zni) sites. The presence of oxygen vacancies on the surface of ZnAcExtrMan_400 and ZnAcPeelMan_400 increased antimicrobial activity, specifically against Gram-negative bacteria Escherichia coli (E. coli) and Salmonella enteritidis (S. enteritidis). ZnNExtrMan with a minimal inhibitory concentration of 0.156 mg/mL was more effective against Gram-positive bacteria Staphylococcus aureus (S. aureus), revealing a high influence of particle size and shape on antimicrobial activity. In addition, the photocatalytic activity of the ZnO NPs was examined by assessing the degradation of acid green dye in an aqueous solution under UV light irradiation. ZnAcPeelMan_400 exhibited excellent photocatalytic activity (94%) within 90 min after irradiation compared to other obtained ZnO NPs.
T2  - ACS Applied Bio Materials
T1  - An Eco-friendly Approach to ZnO NP Synthesis Using Citrus reticulata Blanco Peel/Extract: Characterization and Antibacterial and Photocatalytic Activity
DO  - 10.1021/acsabm.4c00079
ER  - 

@article{
author = "Vasiljevic, Zorka and Vunduk, Jovana and Bartolić, Dragana and Mišković, Goran and Ognjanović, Miloš and Tadić, Nenad B. and Nikolić, Maria Vesna",
year = "2024",
abstract = "Emission of greenhouse gases and infectious diseases caused by improper agro-waste disposal has gained significant attention in recent years. To overcome these hurdles, agro-waste can be valorized into valuable bioactive compounds that act as reducing or stabilizing agents in the synthesis of nanomaterials. Herein, we report a simple circular approach using Citrus reticulata Blanco (C. reticulata) waste (peel powder/aqueous extract) as green reducing and capping/stabilizing agents and Zn nitrate/acetate precursors to synthesize ZnO nanoparticles (NPs) with efficient antimicrobial and photocatalytic activities. The obtained NPs crystallized in a hexagonal wurtzite structure and differed clearly in their morphology. UV-vis analysis of the nanoparticles showed a characteristic broad absorption band between 330 and 414 nm belonging to ZnO NPs. Fourier transform infrared (FTIR) spectroscopy of ZnO NPs exhibited a Zn-O band close to 450 cm-1. The band gap values were in the range of 2.84-3.14 eV depending on the precursor and agent used. The crystallite size obtained from size-strain plots from measured XRD patterns was between 7 and 26 nm, with strain between 16 and 4%. The highly crystalline nature of obtained ZnO NPs was confirmed by clear ring diffraction patterns and d-spacing values of the observed lattice fringes. ZnNPeelMan_400 and ZnNExtrMan showed good stability, as the zeta potential was found to be around −20 mV, and reduced particle aggregation. Photoluminescence analysis revealed different defects belonging to oxygen vacancies (VO+ and VO+2) and zinc interstitial (Zni) sites. The presence of oxygen vacancies on the surface of ZnAcExtrMan_400 and ZnAcPeelMan_400 increased antimicrobial activity, specifically against Gram-negative bacteria Escherichia coli (E. coli) and Salmonella enteritidis (S. enteritidis). ZnNExtrMan with a minimal inhibitory concentration of 0.156 mg/mL was more effective against Gram-positive bacteria Staphylococcus aureus (S. aureus), revealing a high influence of particle size and shape on antimicrobial activity. In addition, the photocatalytic activity of the ZnO NPs was examined by assessing the degradation of acid green dye in an aqueous solution under UV light irradiation. ZnAcPeelMan_400 exhibited excellent photocatalytic activity (94%) within 90 min after irradiation compared to other obtained ZnO NPs.",
journal = "ACS Applied Bio Materials",
title = "An Eco-friendly Approach to ZnO NP Synthesis Using Citrus reticulata Blanco Peel/Extract: Characterization and Antibacterial and Photocatalytic Activity",
doi = "10.1021/acsabm.4c00079"
}

Vasiljevic, Z., Vunduk, J., Bartolić, D., Mišković, G., Ognjanović, M., Tadić, N. B.,& Nikolić, M. V.. (2024). An Eco-friendly Approach to ZnO NP Synthesis Using Citrus reticulata Blanco Peel/Extract: Characterization and Antibacterial and Photocatalytic Activity. in ACS Applied Bio Materials.
https://doi.org/10.1021/acsabm.4c00079

Vasiljevic Z, Vunduk J, Bartolić D, Mišković G, Ognjanović M, Tadić NB, Nikolić MV. An Eco-friendly Approach to ZnO NP Synthesis Using Citrus reticulata Blanco Peel/Extract: Characterization and Antibacterial and Photocatalytic Activity. in ACS Applied Bio Materials. 2024;.
doi:10.1021/acsabm.4c00079 .

Vasiljevic, Zorka, Vunduk, Jovana, Bartolić, Dragana, Mišković, Goran, Ognjanović, Miloš, Tadić, Nenad B., Nikolić, Maria Vesna, "An Eco-friendly Approach to ZnO NP Synthesis Using Citrus reticulata Blanco Peel/Extract: Characterization and Antibacterial and Photocatalytic Activity" in ACS Applied Bio Materials (2024),
https://doi.org/10.1021/acsabm.4c00079 . .

TY  - CONF
AU  - Vasiljević, Zorka
AU  - Vunduk, Jovana
AU  - Dojčinović, Milena
AU  - Bartolić, Dragana
AU  - Ognjanović, Miloš
AU  - Tadić, Nenad
AU  - Mišković, Goran
AU  - Nikolić, Maria Vesna
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11633
AB  - Green synthesis is a more sustainable option using renewable biomass such as plants as reducing or stabilizing agents compared to toxic chemical compounds. These biological substances also behave as capping agents, which control the size and shape of the nanoparticles. In this work, ZnO nanoparticles (NPs) have been prepared via a simple, low cost and ecofriendly method using citrus fruit peel and extracts as biological reducing agents. Zinc nitrate and zinc acetate were used as a source of zinc ions.XRD analysis revealed the formation of a ZnO wurtzite phase without impurities. Synthesized ZnO NPs with an average electronic band gap ∼3 eV were obtained and found to have round-like, hexagonal-like or needle-like structures depending on precursor type. EDS analysis showed a homogeoneous distribution in Zn and O elements, attributed to single-phase ZnO constituents. Antibacterial and antioxidant activities of synthesized NPs were evaluated. Obtained results showed that ZnO synthesized from nitrate precursors are more effective in inhibiting growth of Salmonella and Staphylococcus Aureus. Antioxidant activity of ZnO NPs determined using CUPRAC and ABTS assays showed higher activity of ZnO obtained using nitrate precursors. The maximum scavenging activity of 90% was observed at the concentration of 10 mg/ml
PB  - Belgrade : Serbian Ceramic Society
C3  - Advanced Ceramics and Application :11th Serbian Ceramic Society Conference : program and the book of abstracts; September 18-20, 2023; Belgrade
T1  - Biosynthesis of ZnO nanoparticles using agro-waste with antibacterial and antioxidant activity
SP  - 43
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11633
ER  - 

@conference{
author = "Vasiljević, Zorka and Vunduk, Jovana and Dojčinović, Milena and Bartolić, Dragana and Ognjanović, Miloš and Tadić, Nenad and Mišković, Goran and Nikolić, Maria Vesna",
year = "2023",
abstract = "Green synthesis is a more sustainable option using renewable biomass such as plants as reducing or stabilizing agents compared to toxic chemical compounds. These biological substances also behave as capping agents, which control the size and shape of the nanoparticles. In this work, ZnO nanoparticles (NPs) have been prepared via a simple, low cost and ecofriendly method using citrus fruit peel and extracts as biological reducing agents. Zinc nitrate and zinc acetate were used as a source of zinc ions.XRD analysis revealed the formation of a ZnO wurtzite phase without impurities. Synthesized ZnO NPs with an average electronic band gap ∼3 eV were obtained and found to have round-like, hexagonal-like or needle-like structures depending on precursor type. EDS analysis showed a homogeoneous distribution in Zn and O elements, attributed to single-phase ZnO constituents. Antibacterial and antioxidant activities of synthesized NPs were evaluated. Obtained results showed that ZnO synthesized from nitrate precursors are more effective in inhibiting growth of Salmonella and Staphylococcus Aureus. Antioxidant activity of ZnO NPs determined using CUPRAC and ABTS assays showed higher activity of ZnO obtained using nitrate precursors. The maximum scavenging activity of 90% was observed at the concentration of 10 mg/ml",
publisher = "Belgrade : Serbian Ceramic Society",
journal = "Advanced Ceramics and Application :11th Serbian Ceramic Society Conference : program and the book of abstracts; September 18-20, 2023; Belgrade",
title = "Biosynthesis of ZnO nanoparticles using agro-waste with antibacterial and antioxidant activity",
pages = "43",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11633"
}

Vasiljević, Z., Vunduk, J., Dojčinović, M., Bartolić, D., Ognjanović, M., Tadić, N., Mišković, G.,& Nikolić, M. V.. (2023). Biosynthesis of ZnO nanoparticles using agro-waste with antibacterial and antioxidant activity. in Advanced Ceramics and Application :11th Serbian Ceramic Society Conference : program and the book of abstracts; September 18-20, 2023; Belgrade
Belgrade : Serbian Ceramic Society., 43.
https://hdl.handle.net/21.15107/rcub_vinar_11633

Vasiljević Z, Vunduk J, Dojčinović M, Bartolić D, Ognjanović M, Tadić N, Mišković G, Nikolić MV. Biosynthesis of ZnO nanoparticles using agro-waste with antibacterial and antioxidant activity. in Advanced Ceramics and Application :11th Serbian Ceramic Society Conference : program and the book of abstracts; September 18-20, 2023; Belgrade. 2023;:43.
https://hdl.handle.net/21.15107/rcub_vinar_11633 .

Vasiljević, Zorka, Vunduk, Jovana, Dojčinović, Milena, Bartolić, Dragana, Ognjanović, Miloš, Tadić, Nenad, Mišković, Goran, Nikolić, Maria Vesna, "Biosynthesis of ZnO nanoparticles using agro-waste with antibacterial and antioxidant activity" in Advanced Ceramics and Application :11th Serbian Ceramic Society Conference : program and the book of abstracts; September 18-20, 2023; Belgrade (2023):43,
https://hdl.handle.net/21.15107/rcub_vinar_11633 .

TY  - CONF
AU  - Vasiljević, Zorka
AU  - Vunduk, Jovana
AU  - Dojčinović, Milena
AU  - Bartolić, Dragana
AU  - Ognjanović, Miloš
AU  - Tadić, Nenad
AU  - Mišković, Goran
AU  - Nikolić, Maria Vesna
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11676
AB  - Potential applications in wastewater treatment, energy storage, sensors, food packaging, etc. To date, these materials have been synthesized by different chemical and physical techniques. However many of them employ environmentally unfriendly solvents and toxic chemical compounds. To tackle this problem, use of renewable biomass such as plants and fungi as reducing or stabilizing agents in green synthesis has been considered as more sustainable option compared to toxic chemical compounds. These biological substances also behave as capping agent, which control the size and the shape of the nanoparticles. In this work, ZnO nanoparticles (NPs) have been prepared via simple, low cost and ecofriendly method using citrus fruit peel and extracts, Agaricus bisporus powder and extract as biological reducing agents. Zinc nitrate and zinc acetate were used as source of zinc ions. Structural and optical properties were investigated by X-ray diffraction analysis (XRD), Zeta potential, Fourier Transform Infrared (FTIR) spectroscopy, UV-visible (UV-vis) spectroscopy and Photoluminescence spectroscopy (PL). Morphological features were characterized by Field Emission Scanning Electron microscopy (FESEM) and High Resolution Transmission Electron Microscopy (HRTEM). Antibacterial and antioxidant activity was tested and evaluated.
PB  - Novi Sad : University of Novi Sad, Faculty of Technology
C3  - 2nd International Conference on Advanced Production and Processing : the book of abstracts; Oct 20-22, Novi Sad
T1  - Green biosynthesis of ZnO nanoparticles using agro-waste and their antibacterial and antioxidant activity
SP  - 106
EP  - 106
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11676
ER  - 

@conference{
author = "Vasiljević, Zorka and Vunduk, Jovana and Dojčinović, Milena and Bartolić, Dragana and Ognjanović, Miloš and Tadić, Nenad and Mišković, Goran and Nikolić, Maria Vesna",
year = "2022",
abstract = "Potential applications in wastewater treatment, energy storage, sensors, food packaging, etc. To date, these materials have been synthesized by different chemical and physical techniques. However many of them employ environmentally unfriendly solvents and toxic chemical compounds. To tackle this problem, use of renewable biomass such as plants and fungi as reducing or stabilizing agents in green synthesis has been considered as more sustainable option compared to toxic chemical compounds. These biological substances also behave as capping agent, which control the size and the shape of the nanoparticles. In this work, ZnO nanoparticles (NPs) have been prepared via simple, low cost and ecofriendly method using citrus fruit peel and extracts, Agaricus bisporus powder and extract as biological reducing agents. Zinc nitrate and zinc acetate were used as source of zinc ions. Structural and optical properties were investigated by X-ray diffraction analysis (XRD), Zeta potential, Fourier Transform Infrared (FTIR) spectroscopy, UV-visible (UV-vis) spectroscopy and Photoluminescence spectroscopy (PL). Morphological features were characterized by Field Emission Scanning Electron microscopy (FESEM) and High Resolution Transmission Electron Microscopy (HRTEM). Antibacterial and antioxidant activity was tested and evaluated.",
publisher = "Novi Sad : University of Novi Sad, Faculty of Technology",
journal = "2nd International Conference on Advanced Production and Processing : the book of abstracts; Oct 20-22, Novi Sad",
title = "Green biosynthesis of ZnO nanoparticles using agro-waste and their antibacterial and antioxidant activity",
pages = "106-106",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11676"
}

Vasiljević, Z., Vunduk, J., Dojčinović, M., Bartolić, D., Ognjanović, M., Tadić, N., Mišković, G.,& Nikolić, M. V.. (2022). Green biosynthesis of ZnO nanoparticles using agro-waste and their antibacterial and antioxidant activity. in 2nd International Conference on Advanced Production and Processing : the book of abstracts; Oct 20-22, Novi Sad
Novi Sad : University of Novi Sad, Faculty of Technology., 106-106.
https://hdl.handle.net/21.15107/rcub_vinar_11676

Vasiljević Z, Vunduk J, Dojčinović M, Bartolić D, Ognjanović M, Tadić N, Mišković G, Nikolić MV. Green biosynthesis of ZnO nanoparticles using agro-waste and their antibacterial and antioxidant activity. in 2nd International Conference on Advanced Production and Processing : the book of abstracts; Oct 20-22, Novi Sad. 2022;:106-106.
https://hdl.handle.net/21.15107/rcub_vinar_11676 .

Vasiljević, Zorka, Vunduk, Jovana, Dojčinović, Milena, Bartolić, Dragana, Ognjanović, Miloš, Tadić, Nenad, Mišković, Goran, Nikolić, Maria Vesna, "Green biosynthesis of ZnO nanoparticles using agro-waste and their antibacterial and antioxidant activity" in 2nd International Conference on Advanced Production and Processing : the book of abstracts; Oct 20-22, Novi Sad (2022):106-106,
https://hdl.handle.net/21.15107/rcub_vinar_11676 .