Đurić, Tamara

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orcid::0000-0002-9857-9828
  • Đurić, Tamara (61)
  • Đurić-Delić, Tamara (1)
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Author's Bibliography

Tag Variants of LGALS-3 Containing Haplotype Block in Advanced Carotid Atherosclerosis

Đorđević, Ana; Živković, Maja; Končar, Igor; Stanković, Aleksandra; Kuveljić, Jovana; Đurić, Tamara

(2022)

TY  - JOUR
AU  - Đorđević, Ana
AU  - Živković, Maja
AU  - Končar, Igor
AU  - Stanković, Aleksandra
AU  - Kuveljić, Jovana
AU  - Đurić, Tamara
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10046
AB  - ObjectivesGalectin-3 affects a variety of biological processes. It is encoded by LGALS-3, located in unique haplotype block in Caucasians. Most of the studies regarding the gal-3 role in atherosclerosis are focused exclusively on protein/mRNA levels. Genetic analyses of LGALS-3 are scarce. We sought to thoroughly examine the genetic background of gal-3 and to analyze tag variants that cover more than 80% variability of the LGALS-3 containing hap-block in association with carotid plaque presence (CPP). According to Tagger server, rs4040064 G/T, rs11628437 G/A and rs7159490 C/T cover 82% (r2 > 0.8) of the genetic variance of this hap-block. Our aims were to investigate possible association of rs4040064, rs11628437 and rs7159490 haplotypes with CPP in patients with advanced carotid atherosclerosis (CA) and to analyze their possible effect on LGALS-3 mRNA expression in carotid plaques.Materials and methodsStudy group consisted of 468 patients and 296 controls. Rs4040064, rs11628437, rs7159490 and LGALS-3 mRNA expression were detected by TaqMan® technology.ResultsWe have found that haplotype TAC was associated with the cerebrovascular insult (CVI) occurrence (OR = 1.68, 95% CI = 1.09-2.58, p = 0.02), compared to the referent haplotype. OR was adjusted for hypertension, age and BMI. TAC also showed higher, but not statistically significant, LGALS-3 expression in carotid plaques.ConclusionsOur results suggest that rs4040064, rs11628437 and rs7159490 bear no association with CPP, neither they affect LGALS-3 mRNA in carotid plaques. However, we showed a significant association of haplotype TAC with the CVI occurrence in CA patients from Serbia. Replication and validation of our results are required.
T2  - Journal of Stroke and Cerebrovascular Diseases
T2  - Journal of Stroke and Cerebrovascular DiseasesJournal of Stroke and Cerebrovascular Diseases
T1  - Tag Variants of LGALS-3 Containing Haplotype Block in Advanced Carotid Atherosclerosis
VL  - 31
IS  - 1
SP  - 106212
DO  - 10.1016/j.jstrokecerebrovasdis.2021.106212
ER  - 
@article{
author = "Đorđević, Ana and Živković, Maja and Končar, Igor and Stanković, Aleksandra and Kuveljić, Jovana and Đurić, Tamara",
year = "2022",
abstract = "ObjectivesGalectin-3 affects a variety of biological processes. It is encoded by LGALS-3, located in unique haplotype block in Caucasians. Most of the studies regarding the gal-3 role in atherosclerosis are focused exclusively on protein/mRNA levels. Genetic analyses of LGALS-3 are scarce. We sought to thoroughly examine the genetic background of gal-3 and to analyze tag variants that cover more than 80% variability of the LGALS-3 containing hap-block in association with carotid plaque presence (CPP). According to Tagger server, rs4040064 G/T, rs11628437 G/A and rs7159490 C/T cover 82% (r2 > 0.8) of the genetic variance of this hap-block. Our aims were to investigate possible association of rs4040064, rs11628437 and rs7159490 haplotypes with CPP in patients with advanced carotid atherosclerosis (CA) and to analyze their possible effect on LGALS-3 mRNA expression in carotid plaques.Materials and methodsStudy group consisted of 468 patients and 296 controls. Rs4040064, rs11628437, rs7159490 and LGALS-3 mRNA expression were detected by TaqMan® technology.ResultsWe have found that haplotype TAC was associated with the cerebrovascular insult (CVI) occurrence (OR = 1.68, 95% CI = 1.09-2.58, p = 0.02), compared to the referent haplotype. OR was adjusted for hypertension, age and BMI. TAC also showed higher, but not statistically significant, LGALS-3 expression in carotid plaques.ConclusionsOur results suggest that rs4040064, rs11628437 and rs7159490 bear no association with CPP, neither they affect LGALS-3 mRNA in carotid plaques. However, we showed a significant association of haplotype TAC with the CVI occurrence in CA patients from Serbia. Replication and validation of our results are required.",
journal = "Journal of Stroke and Cerebrovascular Diseases, Journal of Stroke and Cerebrovascular DiseasesJournal of Stroke and Cerebrovascular Diseases",
title = "Tag Variants of LGALS-3 Containing Haplotype Block in Advanced Carotid Atherosclerosis",
volume = "31",
number = "1",
pages = "106212",
doi = "10.1016/j.jstrokecerebrovasdis.2021.106212"
}
Đorđević, A., Živković, M., Končar, I., Stanković, A., Kuveljić, J.,& Đurić, T.. (2022). Tag Variants of LGALS-3 Containing Haplotype Block in Advanced Carotid Atherosclerosis. in Journal of Stroke and Cerebrovascular Diseases, 31(1), 106212.
https://doi.org/10.1016/j.jstrokecerebrovasdis.2021.106212
Đorđević A, Živković M, Končar I, Stanković A, Kuveljić J, Đurić T. Tag Variants of LGALS-3 Containing Haplotype Block in Advanced Carotid Atherosclerosis. in Journal of Stroke and Cerebrovascular Diseases. 2022;31(1):106212.
doi:10.1016/j.jstrokecerebrovasdis.2021.106212 .
Đorđević, Ana, Živković, Maja, Končar, Igor, Stanković, Aleksandra, Kuveljić, Jovana, Đurić, Tamara, "Tag Variants of LGALS-3 Containing Haplotype Block in Advanced Carotid Atherosclerosis" in Journal of Stroke and Cerebrovascular Diseases, 31, no. 1 (2022):106212,
https://doi.org/10.1016/j.jstrokecerebrovasdis.2021.106212 . .

Association of PHACTR1 intronic variants with the first myocardial infarction and their effect on PHACTR1 mRNA expression in PBMCs

Kuveljić, Jovana; Đurić, Tamara; Stanković, Goran; Dekleva, Milica; Stanković, Aleksandra; Alavantić, Dragan; Živković, Maja

(2021)

TY  - JOUR
AU  - Kuveljić, Jovana
AU  - Đurić, Tamara
AU  - Stanković, Goran
AU  - Dekleva, Milica
AU  - Stanković, Aleksandra
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9541
AB  - Background: Myocardial infarction (MI) and underlining atherosclerosis are the main causes of death worldwide. Phosphatase and actin regulator 1 (PHACTR1) variants have been associated with early onset MI, coronary artery disease and carotid dissection. PHACTR1 mRNA expression has been detected in tissues and cells related to atherosclerosis. Nonetheless, the true effect of PHACTR1 on vascular diseases is still unknown. Our aim was to examine the association of PHACTR1 intronic variants, rs9349379, rs2026458 and rs2876300, with MI and multi-vessel disease (MVD) and to assess their effect on PHACTR1 and EDN1 mRNA expression in PBMCs of patients six months after MI. Methods: The study enrolled 537 patients with the first MI and 310 controls. Gene expression was assessed in 74 patients six months after MI and 37 healthy controls. Rs9349379, rs2026458, rs2876300 and relative mRNA expressions were detected by TaqMan® technology. Results: The significant association between PHACTR1 variants and MI was not found, either individually or in haplotype. A higher frequency of rs2876300G-allele in MVD was rendered not significant after Bonferroni correction. PHACTR1 mRNA was significantly increased in PBMCs of patients six months after MI compared to controls (p = 0.02). Patients that carry ACG haplotype have increased PHACTR1 mRNA expression in PBMCs (p = 0.04). There was no effect of PHACTR1 variants on EDN1 mRNA expression. Conclusion: Our findings suggest that PHACTR1 intronic variants may have a role in severity and progression of coronary atherosclerosis. Future research is needed to clarify the mechanism underlying the role of PHACTR1 in coronary atherosclerosis and MI. © 2021 Elsevier B.V.
T2  - Gene
T1  - Association of PHACTR1 intronic variants with the first myocardial infarction and their effect on PHACTR1 mRNA expression in PBMCs
VL  - 775
SP  - 145428
DO  - 10.1016/j.gene.2021.145428
ER  - 
@article{
author = "Kuveljić, Jovana and Đurić, Tamara and Stanković, Goran and Dekleva, Milica and Stanković, Aleksandra and Alavantić, Dragan and Živković, Maja",
year = "2021",
abstract = "Background: Myocardial infarction (MI) and underlining atherosclerosis are the main causes of death worldwide. Phosphatase and actin regulator 1 (PHACTR1) variants have been associated with early onset MI, coronary artery disease and carotid dissection. PHACTR1 mRNA expression has been detected in tissues and cells related to atherosclerosis. Nonetheless, the true effect of PHACTR1 on vascular diseases is still unknown. Our aim was to examine the association of PHACTR1 intronic variants, rs9349379, rs2026458 and rs2876300, with MI and multi-vessel disease (MVD) and to assess their effect on PHACTR1 and EDN1 mRNA expression in PBMCs of patients six months after MI. Methods: The study enrolled 537 patients with the first MI and 310 controls. Gene expression was assessed in 74 patients six months after MI and 37 healthy controls. Rs9349379, rs2026458, rs2876300 and relative mRNA expressions were detected by TaqMan® technology. Results: The significant association between PHACTR1 variants and MI was not found, either individually or in haplotype. A higher frequency of rs2876300G-allele in MVD was rendered not significant after Bonferroni correction. PHACTR1 mRNA was significantly increased in PBMCs of patients six months after MI compared to controls (p = 0.02). Patients that carry ACG haplotype have increased PHACTR1 mRNA expression in PBMCs (p = 0.04). There was no effect of PHACTR1 variants on EDN1 mRNA expression. Conclusion: Our findings suggest that PHACTR1 intronic variants may have a role in severity and progression of coronary atherosclerosis. Future research is needed to clarify the mechanism underlying the role of PHACTR1 in coronary atherosclerosis and MI. © 2021 Elsevier B.V.",
journal = "Gene",
title = "Association of PHACTR1 intronic variants with the first myocardial infarction and their effect on PHACTR1 mRNA expression in PBMCs",
volume = "775",
pages = "145428",
doi = "10.1016/j.gene.2021.145428"
}
Kuveljić, J., Đurić, T., Stanković, G., Dekleva, M., Stanković, A., Alavantić, D.,& Živković, M.. (2021). Association of PHACTR1 intronic variants with the first myocardial infarction and their effect on PHACTR1 mRNA expression in PBMCs. in Gene, 775, 145428.
https://doi.org/10.1016/j.gene.2021.145428
Kuveljić J, Đurić T, Stanković G, Dekleva M, Stanković A, Alavantić D, Živković M. Association of PHACTR1 intronic variants with the first myocardial infarction and their effect on PHACTR1 mRNA expression in PBMCs. in Gene. 2021;775:145428.
doi:10.1016/j.gene.2021.145428 .
Kuveljić, Jovana, Đurić, Tamara, Stanković, Goran, Dekleva, Milica, Stanković, Aleksandra, Alavantić, Dragan, Živković, Maja, "Association of PHACTR1 intronic variants with the first myocardial infarction and their effect on PHACTR1 mRNA expression in PBMCs" in Gene, 775 (2021):145428,
https://doi.org/10.1016/j.gene.2021.145428 . .

The association of glutathione S-transferase T1 and M1 deletions with myocardial infarction

Živković, Maja; Bubić, Maja; Kolaković, Ana; Dekleva, Milica; Stanković, Goran; Stanković, Aleksandra; Đurić, Tamara

(2021)

TY  - JOUR
AU  - Živković, Maja
AU  - Bubić, Maja
AU  - Kolaković, Ana
AU  - Dekleva, Milica
AU  - Stanković, Goran
AU  - Stanković, Aleksandra
AU  - Đurić, Tamara
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9842
AB  - Glutathione S-transferases (GSTs) are the family of enzymes involved in the second line of defense against oxidative stress (OS). The lack of GSTT1/GSTM1 enzyme quantity or activity, due to the presence of homozygous deletion compromises antioxidative defense resulting in OS. OS is the critical mechanism in the pathophysiology of atherosclerosis, coronary artery disease, and myocardial infarction (MI). The increase in reactive oxygen species together with the process of apoptosis plays a role in left ventricular remodeling (LVR) after MI. The associations of GSTT1 and GSTM1 gene polymorphisms with the risk of MI are inconsistent. The aim was to analyze the association of GSTT1/GSTM1 null genotypes with first MI and LVR 8 months after the MI. The study involved 330 controls and 438 consecutive patients with symptoms and signs of first MI. The subgroup of 150 MI patients was prospectively followed up for 6 months. Evidence of maladaptive LVR was obtained by 2D Doppler echocardiography 3-5 days and 6 months after the MI. A multiplex polymerase chain reaction was used to detect the deletion in GSTT1 and GSTM1 genes. GSTM1 null genotype was significantly and independently associated with first MI (adjusted OR = 1.45 95% CI 1.03-2.03, p = 0.03). Association of double null genotypes with maladaptive LVR in patients 6 months after the first MI was no longer significant after adjustment for factors that differed significantly between patients with and without maladaptive LVR. This study demonstrated the association of GSTM1 null genotypes with the risk of MI in the Serbian population.
T2  - Free Radical Research
T1  - The association of glutathione S-transferase T1 and M1 deletions with myocardial infarction
VL  - 55
IS  - 3
SP  - 267
EP  - 274
DO  - 10.1080/10715762.2021.1931166
ER  - 
@article{
author = "Živković, Maja and Bubić, Maja and Kolaković, Ana and Dekleva, Milica and Stanković, Goran and Stanković, Aleksandra and Đurić, Tamara",
year = "2021",
abstract = "Glutathione S-transferases (GSTs) are the family of enzymes involved in the second line of defense against oxidative stress (OS). The lack of GSTT1/GSTM1 enzyme quantity or activity, due to the presence of homozygous deletion compromises antioxidative defense resulting in OS. OS is the critical mechanism in the pathophysiology of atherosclerosis, coronary artery disease, and myocardial infarction (MI). The increase in reactive oxygen species together with the process of apoptosis plays a role in left ventricular remodeling (LVR) after MI. The associations of GSTT1 and GSTM1 gene polymorphisms with the risk of MI are inconsistent. The aim was to analyze the association of GSTT1/GSTM1 null genotypes with first MI and LVR 8 months after the MI. The study involved 330 controls and 438 consecutive patients with symptoms and signs of first MI. The subgroup of 150 MI patients was prospectively followed up for 6 months. Evidence of maladaptive LVR was obtained by 2D Doppler echocardiography 3-5 days and 6 months after the MI. A multiplex polymerase chain reaction was used to detect the deletion in GSTT1 and GSTM1 genes. GSTM1 null genotype was significantly and independently associated with first MI (adjusted OR = 1.45 95% CI 1.03-2.03, p = 0.03). Association of double null genotypes with maladaptive LVR in patients 6 months after the first MI was no longer significant after adjustment for factors that differed significantly between patients with and without maladaptive LVR. This study demonstrated the association of GSTM1 null genotypes with the risk of MI in the Serbian population.",
journal = "Free Radical Research",
title = "The association of glutathione S-transferase T1 and M1 deletions with myocardial infarction",
volume = "55",
number = "3",
pages = "267-274",
doi = "10.1080/10715762.2021.1931166"
}
Živković, M., Bubić, M., Kolaković, A., Dekleva, M., Stanković, G., Stanković, A.,& Đurić, T.. (2021). The association of glutathione S-transferase T1 and M1 deletions with myocardial infarction. in Free Radical Research, 55(3), 267-274.
https://doi.org/10.1080/10715762.2021.1931166
Živković M, Bubić M, Kolaković A, Dekleva M, Stanković G, Stanković A, Đurić T. The association of glutathione S-transferase T1 and M1 deletions with myocardial infarction. in Free Radical Research. 2021;55(3):267-274.
doi:10.1080/10715762.2021.1931166 .
Živković, Maja, Bubić, Maja, Kolaković, Ana, Dekleva, Milica, Stanković, Goran, Stanković, Aleksandra, Đurić, Tamara, "The association of glutathione S-transferase T1 and M1 deletions with myocardial infarction" in Free Radical Research, 55, no. 3 (2021):267-274,
https://doi.org/10.1080/10715762.2021.1931166 . .

The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk

Stojković, Ljiljana S.; Jovanović, Ivan G.; Živković, Maja; Zec, Manja; Đurić, Tamara; Životić, Ivan; Kuveljić, Jovana; Kolaković, Ana; Kolić, Ivana; Đorđević, Ana; Glibetić, Marija; Alavantić, Dragan; Stanković, Aleksandra

(2020)

TY  - JOUR
AU  - Stojković, Ljiljana S.
AU  - Jovanović, Ivan G.
AU  - Živković, Maja
AU  - Zec, Manja
AU  - Đurić, Tamara
AU  - Životić, Ivan
AU  - Kuveljić, Jovana
AU  - Kolaković, Ana
AU  - Kolić, Ivana
AU  - Đorđević, Ana
AU  - Glibetić, Marija
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8683
AB  - Foods and food products that contain polyphenols are proposed to modulate risk of cardiovascular disease. The aim of this three-arm, crossover, randomized, double-blind, placebo-controlled intervention study was to examine the impact of Aronia melanocarpa juice (AMJ), high-polyphenol (AMJ treatment, 1.17 g/100 mL polyphenols) and low-polyphenol (dAMJ treatment, 0.29 g/100 mL polyphenols) dose, on the transcriptome in peripheral blood mononuclear cells (PBMC) of 19 subjects at cardiovascular risk. Transcriptome data were obtained by microarray. Bioinformatic functional annotation analysis was performed on both the whole transcriptome datasets and the differentially expressed genes (DEGs). Expression of selected DEGs was validated by RT-qPCR. Administration of AMJ and dAMJ treatments during the two consecutive four-week treatment periods had additive effects on PBMC transcriptome profiles, with the most pronounced and specific effect noticed for AMJ in the last treatment period (TP3) of the trial. Between the high-dose and low-dose treatments in TP3, there was a multitude of overlapping DEGs and DEG-enriched biological processes and pathways, which primarily included immunomodulation and regulation of cell proliferation/death. Increased expression of TNF, IL1B, IL8, RGS1, OSM, and DUSP2 in TP3 was confirmed by RT-qPCR. The results suggest the immunomodulatory effects of prolonged habitual consumption of polyphenol-rich aronia juice in individuals at cardiovascular risk.
T2  - Nutrients
T1  - The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk
VL  - 12
IS  - 5
SP  - 1484
DO  - 10.3390/nu12051484
ER  - 
@article{
author = "Stojković, Ljiljana S. and Jovanović, Ivan G. and Živković, Maja and Zec, Manja and Đurić, Tamara and Životić, Ivan and Kuveljić, Jovana and Kolaković, Ana and Kolić, Ivana and Đorđević, Ana and Glibetić, Marija and Alavantić, Dragan and Stanković, Aleksandra",
year = "2020",
abstract = "Foods and food products that contain polyphenols are proposed to modulate risk of cardiovascular disease. The aim of this three-arm, crossover, randomized, double-blind, placebo-controlled intervention study was to examine the impact of Aronia melanocarpa juice (AMJ), high-polyphenol (AMJ treatment, 1.17 g/100 mL polyphenols) and low-polyphenol (dAMJ treatment, 0.29 g/100 mL polyphenols) dose, on the transcriptome in peripheral blood mononuclear cells (PBMC) of 19 subjects at cardiovascular risk. Transcriptome data were obtained by microarray. Bioinformatic functional annotation analysis was performed on both the whole transcriptome datasets and the differentially expressed genes (DEGs). Expression of selected DEGs was validated by RT-qPCR. Administration of AMJ and dAMJ treatments during the two consecutive four-week treatment periods had additive effects on PBMC transcriptome profiles, with the most pronounced and specific effect noticed for AMJ in the last treatment period (TP3) of the trial. Between the high-dose and low-dose treatments in TP3, there was a multitude of overlapping DEGs and DEG-enriched biological processes and pathways, which primarily included immunomodulation and regulation of cell proliferation/death. Increased expression of TNF, IL1B, IL8, RGS1, OSM, and DUSP2 in TP3 was confirmed by RT-qPCR. The results suggest the immunomodulatory effects of prolonged habitual consumption of polyphenol-rich aronia juice in individuals at cardiovascular risk.",
journal = "Nutrients",
title = "The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk",
volume = "12",
number = "5",
pages = "1484",
doi = "10.3390/nu12051484"
}
Stojković, L. S., Jovanović, I. G., Živković, M., Zec, M., Đurić, T., Životić, I., Kuveljić, J., Kolaković, A., Kolić, I., Đorđević, A., Glibetić, M., Alavantić, D.,& Stanković, A.. (2020). The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk. in Nutrients, 12(5), 1484.
https://doi.org/10.3390/nu12051484
Stojković LS, Jovanović IG, Živković M, Zec M, Đurić T, Životić I, Kuveljić J, Kolaković A, Kolić I, Đorđević A, Glibetić M, Alavantić D, Stanković A. The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk. in Nutrients. 2020;12(5):1484.
doi:10.3390/nu12051484 .
Stojković, Ljiljana S., Jovanović, Ivan G., Živković, Maja, Zec, Manja, Đurić, Tamara, Životić, Ivan, Kuveljić, Jovana, Kolaković, Ana, Kolić, Ivana, Đorđević, Ana, Glibetić, Marija, Alavantić, Dragan, Stanković, Aleksandra, "The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk" in Nutrients, 12, no. 5 (2020):1484,
https://doi.org/10.3390/nu12051484 . .
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1
1
1

Perimatrix of middle ear cholesteatoma: A granulation tissue with a specific transcriptomic signature

Jovanović, Ivan G.; Živković, Maja; Đurić, Tamara; Stojković, Ljiljana S.; Ješić, Snežana; Stanković, Aleksandra

(2020)

TY  - JOUR
AU  - Jovanović, Ivan G.
AU  - Živković, Maja
AU  - Đurić, Tamara
AU  - Stojković, Ljiljana S.
AU  - Ješić, Snežana
AU  - Stanković, Aleksandra
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8684
AB  - Objectives/Hypothesis: To establish comprehensive transcriptomic profiles of cholesteatoma perimatrix tissue and granulation tissue from chronic otitis media (COM) that did not develop cholesteatoma, which can indicate molecular pathways involved in the cholesteatoma perimatrix pathology and invasiveness. Study Design: Retrospective Case Series. Methods: Transcriptome data were obtained from cholesteatoma perimatrix tissue and COM granulation tissue by an Illumina iScan microarray. Differentially expressed genes (DEGs) were subsequently analyzed using both bioinformatical functional annotation and network analysis. Expression of candidate genes (MMP9 and LCN2) was validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) on a larger group of samples. Results: Analysis of the transcriptome led to the identification of 169 differentially expressed genes between investigated tissues. Bioinformatic analysis suggested that most significant biological processes involving DEGs were previously described in cholesteatoma pathology. Network analysis identified ERBB2, TFAP2A, and TP63 as major hubs of the DEGs molecular network. Furthermore, it was observed that the cellular component most significantly enriched in DEGs was extracellular space containing 47 DEGs. Using qRT-PCR, it was confirmed that mRNA levels of the major extracellular hub (MMP9) are increased, whereas its interacting molecule (LCN2) mRNA levels were decreased in cholesteatoma perimatrix tissue compared to COM granulation tissue. Conclusions: The current study approach offers an overall look at molecular mechanisms that describe the cholesteatoma entity by focusing exclusively on the perimatrix processes in comparison to COM granulation tissue. The observed differences in gene expression between cholesteatoma perimatrix and COM granulation tissue could suggest novel markers potentially influenced by the perimatrix–matrix molecular interplay, which is not present in COM without cholesteatoma. Level of Evidence: NA. Laryngoscope, 130:E220–E227, 2020. © 2019 The American Laryngological, Rhinological and Otological Society, Inc.
T2  - The Laryngoscope
T1  - Perimatrix of middle ear cholesteatoma: A granulation tissue with a specific transcriptomic signature
VL  - 130
IS  - 4
SP  - E220
EP  - E227
DO  - 10.1002/lary.28084
ER  - 
@article{
author = "Jovanović, Ivan G. and Živković, Maja and Đurić, Tamara and Stojković, Ljiljana S. and Ješić, Snežana and Stanković, Aleksandra",
year = "2020",
abstract = "Objectives/Hypothesis: To establish comprehensive transcriptomic profiles of cholesteatoma perimatrix tissue and granulation tissue from chronic otitis media (COM) that did not develop cholesteatoma, which can indicate molecular pathways involved in the cholesteatoma perimatrix pathology and invasiveness. Study Design: Retrospective Case Series. Methods: Transcriptome data were obtained from cholesteatoma perimatrix tissue and COM granulation tissue by an Illumina iScan microarray. Differentially expressed genes (DEGs) were subsequently analyzed using both bioinformatical functional annotation and network analysis. Expression of candidate genes (MMP9 and LCN2) was validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) on a larger group of samples. Results: Analysis of the transcriptome led to the identification of 169 differentially expressed genes between investigated tissues. Bioinformatic analysis suggested that most significant biological processes involving DEGs were previously described in cholesteatoma pathology. Network analysis identified ERBB2, TFAP2A, and TP63 as major hubs of the DEGs molecular network. Furthermore, it was observed that the cellular component most significantly enriched in DEGs was extracellular space containing 47 DEGs. Using qRT-PCR, it was confirmed that mRNA levels of the major extracellular hub (MMP9) are increased, whereas its interacting molecule (LCN2) mRNA levels were decreased in cholesteatoma perimatrix tissue compared to COM granulation tissue. Conclusions: The current study approach offers an overall look at molecular mechanisms that describe the cholesteatoma entity by focusing exclusively on the perimatrix processes in comparison to COM granulation tissue. The observed differences in gene expression between cholesteatoma perimatrix and COM granulation tissue could suggest novel markers potentially influenced by the perimatrix–matrix molecular interplay, which is not present in COM without cholesteatoma. Level of Evidence: NA. Laryngoscope, 130:E220–E227, 2020. © 2019 The American Laryngological, Rhinological and Otological Society, Inc.",
journal = "The Laryngoscope",
title = "Perimatrix of middle ear cholesteatoma: A granulation tissue with a specific transcriptomic signature",
volume = "130",
number = "4",
pages = "E220-E227",
doi = "10.1002/lary.28084"
}
Jovanović, I. G., Živković, M., Đurić, T., Stojković, L. S., Ješić, S.,& Stanković, A.. (2020). Perimatrix of middle ear cholesteatoma: A granulation tissue with a specific transcriptomic signature. in The Laryngoscope, 130(4), E220-E227.
https://doi.org/10.1002/lary.28084
Jovanović IG, Živković M, Đurić T, Stojković LS, Ješić S, Stanković A. Perimatrix of middle ear cholesteatoma: A granulation tissue with a specific transcriptomic signature. in The Laryngoscope. 2020;130(4):E220-E227.
doi:10.1002/lary.28084 .
Jovanović, Ivan G., Živković, Maja, Đurić, Tamara, Stojković, Ljiljana S., Ješić, Snežana, Stanković, Aleksandra, "Perimatrix of middle ear cholesteatoma: A granulation tissue with a specific transcriptomic signature" in The Laryngoscope, 130, no. 4 (2020):E220-E227,
https://doi.org/10.1002/lary.28084 . .
1
5
4
5

PHACTR1 haplotypes are associated with carotid plaque presence and affect PHACTR1 mRNA expression in carotid plaque tissue

Kuveljić, Jovana; Đurić, Tamara; Stanković, Aleksandra; Končar, Igor; Alavantić, Dragan; Živković, Maja

(2019)

TY  - JOUR
AU  - Kuveljić, Jovana
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Končar, Igor
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8348
AB  - Background: Carotid plaque is a hallmark of advanced carotid atherosclerosis and there is evidence of phosphatase and actin regulator 1 (PHACTR1) involvement in the processes that lead to atherosclerosis. PHACTR1 intronic variants have been associated with coronary artery disease and carotid dissection. Up to date the PHACTR1 haplotypes were not investigated in association with carotid plaque presence (CPP). So, the aims of this study were to investigate possible association of PHACTR1 haplotypes inferred from the intronic variants rs9349379, rs2026458 and rs2876300 with CPP in patients with advanced carotid atherosclerosis and to analyze their possible effect on PHACTR1 relative mRNA expression in carotid plaque tissue specimens. Methods: The study group consisted of 501 patients with evidence of carotid plaque presence admitted for carotid endarterectomy and 310 healthy controls. PHACTR1 rs9349379, rs2026458, rs2876300 and relative mRNA expression were detected by TaqMan® technology. Results: We have found significant and independent association of haplotype ACA with the CPP, compared to the referent haplotype GTA (adjusted OR = 1.54 95% CI = 1.07–2.21, p = 0.02). The OR was adjusted for gender, age, BMI, hypertension and total cholesterol. The relative expression of PHACTR1 mRNA in carotid plaque tissue proved to be significantly higher in carriers of the ACG haplotype compared to the referent haplotype GTA (p = 0.03). Conclusion: Our results suggest that PHACTR1 haplotypes inferred from the variants rs9349379, rs2026458 and rs2876300 affect PHACTR1 mRNA and bear the risk for CPP in patients with advanced carotid atherosclerosis. Further replication and validation studies are inevitable. © 2019 Elsevier B.V.
T2  - Gene
T1  - PHACTR1 haplotypes are associated with carotid plaque presence and affect PHACTR1 mRNA expression in carotid plaque tissue
VL  - 710
SP  - 273
EP  - 278
DO  - 10.1016/j.gene.2019.06.020
ER  - 
@article{
author = "Kuveljić, Jovana and Đurić, Tamara and Stanković, Aleksandra and Končar, Igor and Alavantić, Dragan and Živković, Maja",
year = "2019",
abstract = "Background: Carotid plaque is a hallmark of advanced carotid atherosclerosis and there is evidence of phosphatase and actin regulator 1 (PHACTR1) involvement in the processes that lead to atherosclerosis. PHACTR1 intronic variants have been associated with coronary artery disease and carotid dissection. Up to date the PHACTR1 haplotypes were not investigated in association with carotid plaque presence (CPP). So, the aims of this study were to investigate possible association of PHACTR1 haplotypes inferred from the intronic variants rs9349379, rs2026458 and rs2876300 with CPP in patients with advanced carotid atherosclerosis and to analyze their possible effect on PHACTR1 relative mRNA expression in carotid plaque tissue specimens. Methods: The study group consisted of 501 patients with evidence of carotid plaque presence admitted for carotid endarterectomy and 310 healthy controls. PHACTR1 rs9349379, rs2026458, rs2876300 and relative mRNA expression were detected by TaqMan® technology. Results: We have found significant and independent association of haplotype ACA with the CPP, compared to the referent haplotype GTA (adjusted OR = 1.54 95% CI = 1.07–2.21, p = 0.02). The OR was adjusted for gender, age, BMI, hypertension and total cholesterol. The relative expression of PHACTR1 mRNA in carotid plaque tissue proved to be significantly higher in carriers of the ACG haplotype compared to the referent haplotype GTA (p = 0.03). Conclusion: Our results suggest that PHACTR1 haplotypes inferred from the variants rs9349379, rs2026458 and rs2876300 affect PHACTR1 mRNA and bear the risk for CPP in patients with advanced carotid atherosclerosis. Further replication and validation studies are inevitable. © 2019 Elsevier B.V.",
journal = "Gene",
title = "PHACTR1 haplotypes are associated with carotid plaque presence and affect PHACTR1 mRNA expression in carotid plaque tissue",
volume = "710",
pages = "273-278",
doi = "10.1016/j.gene.2019.06.020"
}
Kuveljić, J., Đurić, T., Stanković, A., Končar, I., Alavantić, D.,& Živković, M.. (2019). PHACTR1 haplotypes are associated with carotid plaque presence and affect PHACTR1 mRNA expression in carotid plaque tissue. in Gene, 710, 273-278.
https://doi.org/10.1016/j.gene.2019.06.020
Kuveljić J, Đurić T, Stanković A, Končar I, Alavantić D, Živković M. PHACTR1 haplotypes are associated with carotid plaque presence and affect PHACTR1 mRNA expression in carotid plaque tissue. in Gene. 2019;710:273-278.
doi:10.1016/j.gene.2019.06.020 .
Kuveljić, Jovana, Đurić, Tamara, Stanković, Aleksandra, Končar, Igor, Alavantić, Dragan, Živković, Maja, "PHACTR1 haplotypes are associated with carotid plaque presence and affect PHACTR1 mRNA expression in carotid plaque tissue" in Gene, 710 (2019):273-278,
https://doi.org/10.1016/j.gene.2019.06.020 . .
1
1
1

CDKN2B gene expression is affected by 9p21.3 rs10757278 in CAD patients, six months after the MI

Životić, Ivan; Đurić, Tamara; Stanković, Aleksandra; Milašinović, Dejan; Stanković, Goran; Dekleva, Milica; Marković-Nikolić, Nataša; Alavantić, Dragan; Živković, Maja

(2019)

TY  - JOUR
AU  - Životić, Ivan
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Milašinović, Dejan
AU  - Stanković, Goran
AU  - Dekleva, Milica
AU  - Marković-Nikolić, Nataša
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8432
AB  - Background: Chromosomal region 9p21.3 is most robustly associated with coronary artery disease (CAD) in western European populations. However, heterogeneity in CAD phenotypes leads to uncertainty whether 9p21.3 is associated with stable and/or acute clinical presentations of CAD. 9p21.3 is rich in regulatory elements, but the underlying mechanisms of its actions in CAD remain unclear. We investigate the association of 9p21.3 two haplotype blocks lead variants (rs10757278 and rs518394) with first-ever non-fatal myocardial infarction (MI) in CAD patients and their association with CDKN2B mRNA expression in peripheral blood mononuclear cells 6 months after the event. Methods: We included CAD patients with sustained first MI (n = 523) and controls (n = 583). Gene expression was assessed in 72 patients 6 months after MI and 43 healthy controls. TaqMan® technology was used for the gene expression and genotyping analysis. Results: CDKN2B mRNA was significantly lower in MI patients compared with the controls (p = 0.002) and in patients carrying the rs10757278 G risk allele versus AA homozygotes (p = 0.012) 6 months after the event. While we confirmed the association of rs10757278 with CDKN2B expression in MI patients, we failed to find an association between the investigated variants and MI or disease burden. Conclusions: We suggest a dysregulation of gene expression in the 9p21.3 region six months after acute MI, which is affected by a genetic variant in patients. The rs10757278 rare allele is one factor that might lead to prolonged risk for proatherogenic complications. © 2019 The Canadian Society of Clinical Chemists
T2  - Clinical Biochemistry
T1  - CDKN2B gene expression is affected by 9p21.3 rs10757278 in CAD patients, six months after the MI
VL  - 73
SP  - 70
EP  - 76
DO  - 10.1016/j.clinbiochem.2019.08.003
ER  - 
@article{
author = "Životić, Ivan and Đurić, Tamara and Stanković, Aleksandra and Milašinović, Dejan and Stanković, Goran and Dekleva, Milica and Marković-Nikolić, Nataša and Alavantić, Dragan and Živković, Maja",
year = "2019",
abstract = "Background: Chromosomal region 9p21.3 is most robustly associated with coronary artery disease (CAD) in western European populations. However, heterogeneity in CAD phenotypes leads to uncertainty whether 9p21.3 is associated with stable and/or acute clinical presentations of CAD. 9p21.3 is rich in regulatory elements, but the underlying mechanisms of its actions in CAD remain unclear. We investigate the association of 9p21.3 two haplotype blocks lead variants (rs10757278 and rs518394) with first-ever non-fatal myocardial infarction (MI) in CAD patients and their association with CDKN2B mRNA expression in peripheral blood mononuclear cells 6 months after the event. Methods: We included CAD patients with sustained first MI (n = 523) and controls (n = 583). Gene expression was assessed in 72 patients 6 months after MI and 43 healthy controls. TaqMan® technology was used for the gene expression and genotyping analysis. Results: CDKN2B mRNA was significantly lower in MI patients compared with the controls (p = 0.002) and in patients carrying the rs10757278 G risk allele versus AA homozygotes (p = 0.012) 6 months after the event. While we confirmed the association of rs10757278 with CDKN2B expression in MI patients, we failed to find an association between the investigated variants and MI or disease burden. Conclusions: We suggest a dysregulation of gene expression in the 9p21.3 region six months after acute MI, which is affected by a genetic variant in patients. The rs10757278 rare allele is one factor that might lead to prolonged risk for proatherogenic complications. © 2019 The Canadian Society of Clinical Chemists",
journal = "Clinical Biochemistry",
title = "CDKN2B gene expression is affected by 9p21.3 rs10757278 in CAD patients, six months after the MI",
volume = "73",
pages = "70-76",
doi = "10.1016/j.clinbiochem.2019.08.003"
}
Životić, I., Đurić, T., Stanković, A., Milašinović, D., Stanković, G., Dekleva, M., Marković-Nikolić, N., Alavantić, D.,& Živković, M.. (2019). CDKN2B gene expression is affected by 9p21.3 rs10757278 in CAD patients, six months after the MI. in Clinical Biochemistry, 73, 70-76.
https://doi.org/10.1016/j.clinbiochem.2019.08.003
Životić I, Đurić T, Stanković A, Milašinović D, Stanković G, Dekleva M, Marković-Nikolić N, Alavantić D, Živković M. CDKN2B gene expression is affected by 9p21.3 rs10757278 in CAD patients, six months after the MI. in Clinical Biochemistry. 2019;73:70-76.
doi:10.1016/j.clinbiochem.2019.08.003 .
Životić, Ivan, Đurić, Tamara, Stanković, Aleksandra, Milašinović, Dejan, Stanković, Goran, Dekleva, Milica, Marković-Nikolić, Nataša, Alavantić, Dragan, Živković, Maja, "CDKN2B gene expression is affected by 9p21.3 rs10757278 in CAD patients, six months after the MI" in Clinical Biochemistry, 73 (2019):70-76,
https://doi.org/10.1016/j.clinbiochem.2019.08.003 . .
3
3
3

Left ventricular remodeling after the first myocardial infarction in association with LGALS-3 neighbouring variants rs2274273 and rs17128183 and its relative mRNA expression: a prospective study

Đorđević, Ana D.; Dekleva, Milica; Živković, Maja; Stanković, Aleksandra; Marković-Nikolić, Nataša; Alavantić, Dragan; Đurić, Tamara

(2018)

TY  - JOUR
AU  - Đorđević, Ana D.
AU  - Dekleva, Milica
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Marković-Nikolić, Nataša
AU  - Alavantić, Dragan
AU  - Đurić, Tamara
PY  - 2018
UR  - http://link.springer.com/10.1007/s11033-018-4384-4
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7958
AB  - Post-infarct left ventricular remodeling (LVR) process increases the risk of heart failure (HF). Circulating galectin-3 has been associated with fibrosis, inflammation and cardiac dysfunction during the remodeling process after myocardial infarction (MI). The aims of this prospective case study were to investigate the association of potentially functional variants in the vicinity of LGALS-3 locus, rs2274273 and rs17128183 with maladaptive LVR and whether these variants could affect LGALS-3 mRNA expression in peripheral blood mononuclear cells of patients 6 months after the first MI. This study encompassed 167 patients with acute MI that were followed up for 6 months. Evidence of LVR was obtained by repeated 2D Doppler echocardiography. Rs2274273, rs17128183 and LGALS-3 mRNA expression were detected by TaqMan® technology. Rs2274273 and rs17128183 rare allele bearing genotypes, according to the dominant model (CT+TT vs. CC and AG+GG vs. AA, respectively), were significantly and independently associated with maladaptive LVR (adjusted OR = 3.02, P = 0.016; adjusted OR = 3.14, P = 0.019, respectively) and higher LGALS-3 mRNA expression (fold induction 1.203, P = 0.03 and 1.214, P = 0.03, respectively). Our exploratory results suggest that rs2274273 and rs17128183 variants affect LGALS-3 mRNA and bear the risk for maladaptive LVR post-MI remodeling. Further replication and validation in a larger group of patients is inevitable. © 2018, Springer Nature B.V.
T2  - Molecular Biology Reports
T1  - Left ventricular remodeling after the first myocardial infarction in association with LGALS-3 neighbouring variants rs2274273 and rs17128183 and its relative mRNA expression: a prospective study
VL  - 45
IS  - 6
SP  - 2227
EP  - 2236
DO  - 10.1007/s11033-018-4384-4
ER  - 
@article{
author = "Đorđević, Ana D. and Dekleva, Milica and Živković, Maja and Stanković, Aleksandra and Marković-Nikolić, Nataša and Alavantić, Dragan and Đurić, Tamara",
year = "2018",
abstract = "Post-infarct left ventricular remodeling (LVR) process increases the risk of heart failure (HF). Circulating galectin-3 has been associated with fibrosis, inflammation and cardiac dysfunction during the remodeling process after myocardial infarction (MI). The aims of this prospective case study were to investigate the association of potentially functional variants in the vicinity of LGALS-3 locus, rs2274273 and rs17128183 with maladaptive LVR and whether these variants could affect LGALS-3 mRNA expression in peripheral blood mononuclear cells of patients 6 months after the first MI. This study encompassed 167 patients with acute MI that were followed up for 6 months. Evidence of LVR was obtained by repeated 2D Doppler echocardiography. Rs2274273, rs17128183 and LGALS-3 mRNA expression were detected by TaqMan® technology. Rs2274273 and rs17128183 rare allele bearing genotypes, according to the dominant model (CT+TT vs. CC and AG+GG vs. AA, respectively), were significantly and independently associated with maladaptive LVR (adjusted OR = 3.02, P = 0.016; adjusted OR = 3.14, P = 0.019, respectively) and higher LGALS-3 mRNA expression (fold induction 1.203, P = 0.03 and 1.214, P = 0.03, respectively). Our exploratory results suggest that rs2274273 and rs17128183 variants affect LGALS-3 mRNA and bear the risk for maladaptive LVR post-MI remodeling. Further replication and validation in a larger group of patients is inevitable. © 2018, Springer Nature B.V.",
journal = "Molecular Biology Reports",
title = "Left ventricular remodeling after the first myocardial infarction in association with LGALS-3 neighbouring variants rs2274273 and rs17128183 and its relative mRNA expression: a prospective study",
volume = "45",
number = "6",
pages = "2227-2236",
doi = "10.1007/s11033-018-4384-4"
}
Đorđević, A. D., Dekleva, M., Živković, M., Stanković, A., Marković-Nikolić, N., Alavantić, D.,& Đurić, T.. (2018). Left ventricular remodeling after the first myocardial infarction in association with LGALS-3 neighbouring variants rs2274273 and rs17128183 and its relative mRNA expression: a prospective study. in Molecular Biology Reports, 45(6), 2227-2236.
https://doi.org/10.1007/s11033-018-4384-4
Đorđević AD, Dekleva M, Živković M, Stanković A, Marković-Nikolić N, Alavantić D, Đurić T. Left ventricular remodeling after the first myocardial infarction in association with LGALS-3 neighbouring variants rs2274273 and rs17128183 and its relative mRNA expression: a prospective study. in Molecular Biology Reports. 2018;45(6):2227-2236.
doi:10.1007/s11033-018-4384-4 .
Đorđević, Ana D., Dekleva, Milica, Živković, Maja, Stanković, Aleksandra, Marković-Nikolić, Nataša, Alavantić, Dragan, Đurić, Tamara, "Left ventricular remodeling after the first myocardial infarction in association with LGALS-3 neighbouring variants rs2274273 and rs17128183 and its relative mRNA expression: a prospective study" in Molecular Biology Reports, 45, no. 6 (2018):2227-2236,
https://doi.org/10.1007/s11033-018-4384-4 . .
1
5
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6

Transcriptome-driven integrative exploration of functional state of ureter tissue affected by CAKUT

Jovanović, Ivan G.; Živković, Maja; Kostić, Mirjana M.; Krstić, Zoran; Đurić, Tamara; Licastro, Danilo; Meroni, Germana; Alavantić, Dragan; Stanković, Aleksandra

(2018)

TY  - JOUR
AU  - Jovanović, Ivan G.
AU  - Živković, Maja
AU  - Kostić, Mirjana M.
AU  - Krstić, Zoran
AU  - Đurić, Tamara
AU  - Licastro, Danilo
AU  - Meroni, Germana
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0024320518305940
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7891
AB  - Aims: (1) to identify the most dysregulated genes in ureter tissue affected by congenital anomalies of the kidney and urinary tract (CAKUT) and to extract the biological meaning of these markers; (2) to describe the key molecular networks in CAKUT and to provide expression validation of the genes selected from these networks. Main methods: Transcriptome data was obtained from ureter samples of CAKUT patients and controls by Illumina iScan microarray. Identification of differentially expressed genes was coupled with subsequent bioinformatics analyses. Expression of candidate genes was validated by qRT-PCR. Key findings: Analysis of the transcriptome led to the identification of 78 commonly dysregulated genes in CAKUT tissue compared to controls. Integrative bioinformatic analyses of differentially expressed genes identified 7 major networks. The targets for qRT-PCR validation were selected as members of the major molecular networks in CAKUT, which had both, the significant high fold change and biological relevance for CAKUT. By qRT-PCR the substantial increase of LCN2, PROM1, SOSTDC1, and decrease of INA, RASD1 and TAC3 mRNA levels was confirmed. Significance: Since CAKUT is a leading cause of end-stage renal disease in children, the search for molecular targets for postnatal therapy is of particular interest. Data described in this study represents the gene expression profile and significant molecular networks specific to human ureter affected by CAKUT. The discovery of impaired molecular factors and processes is the step towards the uncovering of the key mechanisms that reflect CAKUT postnatally and could lead to the affected tissue deterioration and end organ damage. © 2018 Elsevier Inc.
T2  - Life Sciences
T1  - Transcriptome-driven integrative exploration of functional state of ureter tissue affected by CAKUT
VL  - 212
SP  - 1
EP  - 8
DO  - 10.1016/j.lfs.2018.09.042
ER  - 
@article{
author = "Jovanović, Ivan G. and Živković, Maja and Kostić, Mirjana M. and Krstić, Zoran and Đurić, Tamara and Licastro, Danilo and Meroni, Germana and Alavantić, Dragan and Stanković, Aleksandra",
year = "2018",
abstract = "Aims: (1) to identify the most dysregulated genes in ureter tissue affected by congenital anomalies of the kidney and urinary tract (CAKUT) and to extract the biological meaning of these markers; (2) to describe the key molecular networks in CAKUT and to provide expression validation of the genes selected from these networks. Main methods: Transcriptome data was obtained from ureter samples of CAKUT patients and controls by Illumina iScan microarray. Identification of differentially expressed genes was coupled with subsequent bioinformatics analyses. Expression of candidate genes was validated by qRT-PCR. Key findings: Analysis of the transcriptome led to the identification of 78 commonly dysregulated genes in CAKUT tissue compared to controls. Integrative bioinformatic analyses of differentially expressed genes identified 7 major networks. The targets for qRT-PCR validation were selected as members of the major molecular networks in CAKUT, which had both, the significant high fold change and biological relevance for CAKUT. By qRT-PCR the substantial increase of LCN2, PROM1, SOSTDC1, and decrease of INA, RASD1 and TAC3 mRNA levels was confirmed. Significance: Since CAKUT is a leading cause of end-stage renal disease in children, the search for molecular targets for postnatal therapy is of particular interest. Data described in this study represents the gene expression profile and significant molecular networks specific to human ureter affected by CAKUT. The discovery of impaired molecular factors and processes is the step towards the uncovering of the key mechanisms that reflect CAKUT postnatally and could lead to the affected tissue deterioration and end organ damage. © 2018 Elsevier Inc.",
journal = "Life Sciences",
title = "Transcriptome-driven integrative exploration of functional state of ureter tissue affected by CAKUT",
volume = "212",
pages = "1-8",
doi = "10.1016/j.lfs.2018.09.042"
}
Jovanović, I. G., Živković, M., Kostić, M. M., Krstić, Z., Đurić, T., Licastro, D., Meroni, G., Alavantić, D.,& Stanković, A.. (2018). Transcriptome-driven integrative exploration of functional state of ureter tissue affected by CAKUT. in Life Sciences, 212, 1-8.
https://doi.org/10.1016/j.lfs.2018.09.042
Jovanović IG, Živković M, Kostić MM, Krstić Z, Đurić T, Licastro D, Meroni G, Alavantić D, Stanković A. Transcriptome-driven integrative exploration of functional state of ureter tissue affected by CAKUT. in Life Sciences. 2018;212:1-8.
doi:10.1016/j.lfs.2018.09.042 .
Jovanović, Ivan G., Živković, Maja, Kostić, Mirjana M., Krstić, Zoran, Đurić, Tamara, Licastro, Danilo, Meroni, Germana, Alavantić, Dragan, Stanković, Aleksandra, "Transcriptome-driven integrative exploration of functional state of ureter tissue affected by CAKUT" in Life Sciences, 212 (2018):1-8,
https://doi.org/10.1016/j.lfs.2018.09.042 . .
1
6
4
4

The expression of renin-angiotensin-system components (ACE, ACE2 and collectrin (TMEM27)) in the human carotid plaques depending on gender and plaque phenotype

Kolaković, Ana; Živković, Maja; Đurić, Tamara; Končar, Igor; Stanković, Aleksandra

(2018)

TY  - CONF
AU  - Kolaković, Ana
AU  - Živković, Maja
AU  - Đurić, Tamara
AU  - Končar, Igor
AU  - Stanković, Aleksandra
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S002191501830707X
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7952
C3  - Atherosclerosis
T1  - The expression of renin-angiotensin-system components (ACE, ACE2 and collectrin (TMEM27)) in the human carotid plaques depending on gender and plaque phenotype
VL  - 275
SP  - e135
DO  - 10.1016/j.atherosclerosis.2018.06.395
ER  - 
@conference{
author = "Kolaković, Ana and Živković, Maja and Đurić, Tamara and Končar, Igor and Stanković, Aleksandra",
year = "2018",
journal = "Atherosclerosis",
title = "The expression of renin-angiotensin-system components (ACE, ACE2 and collectrin (TMEM27)) in the human carotid plaques depending on gender and plaque phenotype",
volume = "275",
pages = "e135",
doi = "10.1016/j.atherosclerosis.2018.06.395"
}
Kolaković, A., Živković, M., Đurić, T., Končar, I.,& Stanković, A.. (2018). The expression of renin-angiotensin-system components (ACE, ACE2 and collectrin (TMEM27)) in the human carotid plaques depending on gender and plaque phenotype. in Atherosclerosis, 275, e135.
https://doi.org/10.1016/j.atherosclerosis.2018.06.395
Kolaković A, Živković M, Đurić T, Končar I, Stanković A. The expression of renin-angiotensin-system components (ACE, ACE2 and collectrin (TMEM27)) in the human carotid plaques depending on gender and plaque phenotype. in Atherosclerosis. 2018;275:e135.
doi:10.1016/j.atherosclerosis.2018.06.395 .
Kolaković, Ana, Živković, Maja, Đurić, Tamara, Končar, Igor, Stanković, Aleksandra, "The expression of renin-angiotensin-system components (ACE, ACE2 and collectrin (TMEM27)) in the human carotid plaques depending on gender and plaque phenotype" in Atherosclerosis, 275 (2018):e135,
https://doi.org/10.1016/j.atherosclerosis.2018.06.395 . .
2
2

HACD4 haplotype confers risk of myocardial infarction among males in the population of Serbia

Životić, Ivan; Đurić, Tamara; Stanković, Aleksandra; Stanković, Goran; Milašinović, Dejan; Dekleva, Milica; Marković-Nikolić, Nataša; Alavantić, Dragan; Živković, Maja

(2018)

TY  - CONF
AU  - Životić, Ivan
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Stanković, Goran
AU  - Milašinović, Dejan
AU  - Dekleva, Milica
AU  - Marković-Nikolić, Nataša
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0021915018309699
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7953
C3  - Atherosclerosis
T1  - HACD4 haplotype confers risk of myocardial infarction among males in the population of Serbia
VL  - 275
SP  - e210
EP  - e211
DO  - 10.1016/j.atherosclerosis.2018.06.657
ER  - 
@conference{
author = "Životić, Ivan and Đurić, Tamara and Stanković, Aleksandra and Stanković, Goran and Milašinović, Dejan and Dekleva, Milica and Marković-Nikolić, Nataša and Alavantić, Dragan and Živković, Maja",
year = "2018",
journal = "Atherosclerosis",
title = "HACD4 haplotype confers risk of myocardial infarction among males in the population of Serbia",
volume = "275",
pages = "e210-e211",
doi = "10.1016/j.atherosclerosis.2018.06.657"
}
Životić, I., Đurić, T., Stanković, A., Stanković, G., Milašinović, D., Dekleva, M., Marković-Nikolić, N., Alavantić, D.,& Živković, M.. (2018). HACD4 haplotype confers risk of myocardial infarction among males in the population of Serbia. in Atherosclerosis, 275, e210-e211.
https://doi.org/10.1016/j.atherosclerosis.2018.06.657
Životić I, Đurić T, Stanković A, Stanković G, Milašinović D, Dekleva M, Marković-Nikolić N, Alavantić D, Živković M. HACD4 haplotype confers risk of myocardial infarction among males in the population of Serbia. in Atherosclerosis. 2018;275:e210-e211.
doi:10.1016/j.atherosclerosis.2018.06.657 .
Životić, Ivan, Đurić, Tamara, Stanković, Aleksandra, Stanković, Goran, Milašinović, Dejan, Dekleva, Milica, Marković-Nikolić, Nataša, Alavantić, Dragan, Živković, Maja, "HACD4 haplotype confers risk of myocardial infarction among males in the population of Serbia" in Atherosclerosis, 275 (2018):e210-e211,
https://doi.org/10.1016/j.atherosclerosis.2018.06.657 . .

The HACD4 haplotype as a risk factor for atherosclerosis in males

Životić, Ivan; Đurić, Tamara; Stanković, Aleksandra; Ivancevic, Ilija; Končar, Igor; Milašinović, Dejan; Stanković, Goran; Alavantić, Dragan; Živković, Maja

(2018)

TY  - JOUR
AU  - Životić, Ivan
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Ivancevic, Ilija
AU  - Končar, Igor
AU  - Milašinović, Dejan
AU  - Stanković, Goran
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1843
AB  - The 9p21.3 region is rich in regulatory elements and the variants in this region had been robustly associated with carotid plaque (CP) and coronary artery disease (CAD). Recently, the HACD4 was detected as one of the six 9p21.3 differentially expressed genes associated with accelerated atherosclerosis and greater mean lesion area in the Athsq1 congenic mice. We aimed to investigate association of two potentially regulatory HACD4 variants (rs36212560 I/D, rs2275888 T/C) and their haplotypes with CP occurrence and the level of HACD4 and FOCAD mRNA in human CP tissue. Association study was replicated in CAD patients who suffered the first myocardial infarction. Study included 477 CP patients, 303 healthy controls and replication sample of 224 CAD males from the population of Serbia. Genotypes were determined by polymerase chain reaction (PCR) and real-time PCR using TaqMan (R) technology. The gene expression was detected with TaqMan (R) technology. We have found significant and independent association of DT haplotype with CP presence in men (adjusted OR = 1.64 CI = 1.12-2.42, p = 0.011). The result was replicated in CAD males (adjusted OR = 1.84 CI = 1.21-2.80, p = 0.004). We have found significant effect of the HACD4 rs2275888 on FOCAD mRNA level in human CP tissue. Correction for multiple testing was performed. Independent association of HACD4 haplotypes with atherosclerotic phenotypes connotes a further validation and replication in larger cohorts as well as functional studies to enlighten the potential mechanism of its action in pathophysiology of atherosclerosis.
T2  - Gene
T1  - The HACD4 haplotype as a risk factor for atherosclerosis in males
VL  - 641
SP  - 35
EP  - 40
DO  - 10.1016/j.gene.2017.10.030
ER  - 
@article{
author = "Životić, Ivan and Đurić, Tamara and Stanković, Aleksandra and Ivancevic, Ilija and Končar, Igor and Milašinović, Dejan and Stanković, Goran and Alavantić, Dragan and Živković, Maja",
year = "2018",
abstract = "The 9p21.3 region is rich in regulatory elements and the variants in this region had been robustly associated with carotid plaque (CP) and coronary artery disease (CAD). Recently, the HACD4 was detected as one of the six 9p21.3 differentially expressed genes associated with accelerated atherosclerosis and greater mean lesion area in the Athsq1 congenic mice. We aimed to investigate association of two potentially regulatory HACD4 variants (rs36212560 I/D, rs2275888 T/C) and their haplotypes with CP occurrence and the level of HACD4 and FOCAD mRNA in human CP tissue. Association study was replicated in CAD patients who suffered the first myocardial infarction. Study included 477 CP patients, 303 healthy controls and replication sample of 224 CAD males from the population of Serbia. Genotypes were determined by polymerase chain reaction (PCR) and real-time PCR using TaqMan (R) technology. The gene expression was detected with TaqMan (R) technology. We have found significant and independent association of DT haplotype with CP presence in men (adjusted OR = 1.64 CI = 1.12-2.42, p = 0.011). The result was replicated in CAD males (adjusted OR = 1.84 CI = 1.21-2.80, p = 0.004). We have found significant effect of the HACD4 rs2275888 on FOCAD mRNA level in human CP tissue. Correction for multiple testing was performed. Independent association of HACD4 haplotypes with atherosclerotic phenotypes connotes a further validation and replication in larger cohorts as well as functional studies to enlighten the potential mechanism of its action in pathophysiology of atherosclerosis.",
journal = "Gene",
title = "The HACD4 haplotype as a risk factor for atherosclerosis in males",
volume = "641",
pages = "35-40",
doi = "10.1016/j.gene.2017.10.030"
}
Životić, I., Đurić, T., Stanković, A., Ivancevic, I., Končar, I., Milašinović, D., Stanković, G., Alavantić, D.,& Živković, M.. (2018). The HACD4 haplotype as a risk factor for atherosclerosis in males. in Gene, 641, 35-40.
https://doi.org/10.1016/j.gene.2017.10.030
Životić I, Đurić T, Stanković A, Ivancevic I, Končar I, Milašinović D, Stanković G, Alavantić D, Živković M. The HACD4 haplotype as a risk factor for atherosclerosis in males. in Gene. 2018;641:35-40.
doi:10.1016/j.gene.2017.10.030 .
Životić, Ivan, Đurić, Tamara, Stanković, Aleksandra, Ivancevic, Ilija, Končar, Igor, Milašinović, Dejan, Stanković, Goran, Alavantić, Dragan, Živković, Maja, "The HACD4 haplotype as a risk factor for atherosclerosis in males" in Gene, 641 (2018):35-40,
https://doi.org/10.1016/j.gene.2017.10.030 . .

RS10757278 FROM 9P21 IS ASSOCIATED WITH ST-ELEVATED MYOCARDIAL INFARCTION IN FEMALES IN POPULATION OF SERBIA

Životić, Ivan; Živković, Maja; Đurić, Tamara; Stanković, Aleksandra; Đorđević, Ana D.; Dekleva, Milica; Marković-Nikolić, Nevena; Alavantić, Dragan

(2017)

TY  - CONF
AU  - Životić, Ivan
AU  - Živković, Maja
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Đorđević, Ana D.
AU  - Dekleva, Milica
AU  - Marković-Nikolić, Nevena
AU  - Alavantić, Dragan
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7178
C3  - Atherosclerosis
T1  - RS10757278 FROM 9P21 IS ASSOCIATED WITH ST-ELEVATED MYOCARDIAL INFARCTION IN FEMALES IN POPULATION OF SERBIA
VL  - 263
SP  - E188
EP  - E188
DO  - 10.1016/j.atherosclerosis.2017.06.603
ER  - 
@conference{
author = "Životić, Ivan and Živković, Maja and Đurić, Tamara and Stanković, Aleksandra and Đorđević, Ana D. and Dekleva, Milica and Marković-Nikolić, Nevena and Alavantić, Dragan",
year = "2017",
journal = "Atherosclerosis",
title = "RS10757278 FROM 9P21 IS ASSOCIATED WITH ST-ELEVATED MYOCARDIAL INFARCTION IN FEMALES IN POPULATION OF SERBIA",
volume = "263",
pages = "E188-E188",
doi = "10.1016/j.atherosclerosis.2017.06.603"
}
Životić, I., Živković, M., Đurić, T., Stanković, A., Đorđević, A. D., Dekleva, M., Marković-Nikolić, N.,& Alavantić, D.. (2017). RS10757278 FROM 9P21 IS ASSOCIATED WITH ST-ELEVATED MYOCARDIAL INFARCTION IN FEMALES IN POPULATION OF SERBIA. in Atherosclerosis, 263, E188-E188.
https://doi.org/10.1016/j.atherosclerosis.2017.06.603
Životić I, Živković M, Đurić T, Stanković A, Đorđević AD, Dekleva M, Marković-Nikolić N, Alavantić D. RS10757278 FROM 9P21 IS ASSOCIATED WITH ST-ELEVATED MYOCARDIAL INFARCTION IN FEMALES IN POPULATION OF SERBIA. in Atherosclerosis. 2017;263:E188-E188.
doi:10.1016/j.atherosclerosis.2017.06.603 .
Životić, Ivan, Živković, Maja, Đurić, Tamara, Stanković, Aleksandra, Đorđević, Ana D., Dekleva, Milica, Marković-Nikolić, Nevena, Alavantić, Dragan, "RS10757278 FROM 9P21 IS ASSOCIATED WITH ST-ELEVATED MYOCARDIAL INFARCTION IN FEMALES IN POPULATION OF SERBIA" in Atherosclerosis, 263 (2017):E188-E188,
https://doi.org/10.1016/j.atherosclerosis.2017.06.603 . .

Snps in the Vicinity of Lgals-3 Locus and Different Shape-Types of Heart Remodeling After the First Myocardial Infarction: Preliminary Results

Đorđević, Ana D.; Živković, Maja; Dekleva, Milica; Marković-Nikolić, Nataša; Alavantić, Dragan; Stanković, Aleksandra; Đurić, Tamara

(2017)

TY  - CONF
AU  - Đorđević, Ana D.
AU  - Živković, Maja
AU  - Dekleva, Milica
AU  - Marković-Nikolić, Nataša
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
AU  - Đurić, Tamara
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7177
C3  - Atherosclerosis
T1  - Snps in the Vicinity of Lgals-3 Locus and Different Shape-Types of Heart Remodeling After the First Myocardial Infarction: Preliminary Results
VL  - 263
SP  - E180
EP  - E180
DO  - 10.1016/j.atherosclerosis.2017.06.578
ER  - 
@conference{
author = "Đorđević, Ana D. and Živković, Maja and Dekleva, Milica and Marković-Nikolić, Nataša and Alavantić, Dragan and Stanković, Aleksandra and Đurić, Tamara",
year = "2017",
journal = "Atherosclerosis",
title = "Snps in the Vicinity of Lgals-3 Locus and Different Shape-Types of Heart Remodeling After the First Myocardial Infarction: Preliminary Results",
volume = "263",
pages = "E180-E180",
doi = "10.1016/j.atherosclerosis.2017.06.578"
}
Đorđević, A. D., Živković, M., Dekleva, M., Marković-Nikolić, N., Alavantić, D., Stanković, A.,& Đurić, T.. (2017). Snps in the Vicinity of Lgals-3 Locus and Different Shape-Types of Heart Remodeling After the First Myocardial Infarction: Preliminary Results. in Atherosclerosis, 263, E180-E180.
https://doi.org/10.1016/j.atherosclerosis.2017.06.578
Đorđević AD, Živković M, Dekleva M, Marković-Nikolić N, Alavantić D, Stanković A, Đurić T. Snps in the Vicinity of Lgals-3 Locus and Different Shape-Types of Heart Remodeling After the First Myocardial Infarction: Preliminary Results. in Atherosclerosis. 2017;263:E180-E180.
doi:10.1016/j.atherosclerosis.2017.06.578 .
Đorđević, Ana D., Živković, Maja, Dekleva, Milica, Marković-Nikolić, Nataša, Alavantić, Dragan, Stanković, Aleksandra, Đurić, Tamara, "Snps in the Vicinity of Lgals-3 Locus and Different Shape-Types of Heart Remodeling After the First Myocardial Infarction: Preliminary Results" in Atherosclerosis, 263 (2017):E180-E180,
https://doi.org/10.1016/j.atherosclerosis.2017.06.578 . .

Heart failure development and rs2274273 in the vicinity of LGALS-3 locus, LGALS-3 relative mRNA expression in patients with first myocardial infarction

Dekleva, Milica; Đorđević, Ana D.; Živković, Maja; Stanković, Aleksandra; Nikolic, N. M. Markovic; Đurić, Tamara

(2017)

TY  - CONF
AU  - Dekleva, Milica
AU  - Đorđević, Ana D.
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Nikolic, N. M. Markovic
AU  - Đurić, Tamara
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1564
C3  - European Journal of Heart Failure
T1  - Heart failure development and rs2274273 in the vicinity of LGALS-3 locus, LGALS-3 relative mRNA expression in patients with first myocardial infarction
VL  - 19
IS  - SI
SP  - 552
EP  - 552
ER  - 
@conference{
author = "Dekleva, Milica and Đorđević, Ana D. and Živković, Maja and Stanković, Aleksandra and Nikolic, N. M. Markovic and Đurić, Tamara",
year = "2017",
journal = "European Journal of Heart Failure",
title = "Heart failure development and rs2274273 in the vicinity of LGALS-3 locus, LGALS-3 relative mRNA expression in patients with first myocardial infarction",
volume = "19",
number = "SI",
pages = "552-552"
}
Dekleva, M., Đorđević, A. D., Živković, M., Stanković, A., Nikolic, N. M. M.,& Đurić, T.. (2017). Heart failure development and rs2274273 in the vicinity of LGALS-3 locus, LGALS-3 relative mRNA expression in patients with first myocardial infarction. in European Journal of Heart Failure, 19(SI), 552-552.
Dekleva M, Đorđević AD, Živković M, Stanković A, Nikolic NMM, Đurić T. Heart failure development and rs2274273 in the vicinity of LGALS-3 locus, LGALS-3 relative mRNA expression in patients with first myocardial infarction. in European Journal of Heart Failure. 2017;19(SI):552-552..
Dekleva, Milica, Đorđević, Ana D., Živković, Maja, Stanković, Aleksandra, Nikolic, N. M. Markovic, Đurić, Tamara, "Heart failure development and rs2274273 in the vicinity of LGALS-3 locus, LGALS-3 relative mRNA expression in patients with first myocardial infarction" in European Journal of Heart Failure, 19, no. SI (2017):552-552.

Genetic Variants in the Vicinity of LGALS-3 Gene and LGALS-3 mRNA Expression in Advanced Carotid Atherosclerosis: An Exploratory Study

Đorđević, Ana D.; Živković, Maja; Stanković, Aleksandra; Životić, Ivan; Končar, Igor; Davidović, Lazar; Alavantić, Dragan; Đurić, Tamara

(2016)

TY  - JOUR
AU  - Đorđević, Ana D.
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Životić, Ivan
AU  - Končar, Igor
AU  - Davidović, Lazar
AU  - Alavantić, Dragan
AU  - Đurić, Tamara
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1291
AB  - BackgroundPrevious research has shown that there is an association between galectin-3 (gal-3) protein and cardiovascular pathology. The aim of this study was to investigate the effects of rs2274273 and rs17128183 on genetic susceptibility to advanced carotid atherosclerosis (CA) and its complications. The rs2274273 has been singled out as the lead SNP of the haplotype block containing LGALS-3 (gal-3 gene) associated with gal-3 circulating levels, while rs17128183 constitutes a potentially functional SNP of the same hap-block. We further sought to determine whether these genetic variants have an impact on the expression of LGALS-3 mRNA in human carotid atherosclerotic plaque tissue. MethodsThe study encompassed 300 control subjects and 485 patients with advanced CA who had undergone carotid endarterectomy. Rs2274273, rs17128183, and LGALS-3 relative mRNA expression was detected by means of real-time PCR (TaqMan((R)) technology). ResultsThere were no statistically significant associations of the investigated genetic variants with susceptibility to advanced CA, nor did we find any associations in terms of ultrasonographically defined plaque phenotypes. The relative expression of LGALS-3 mRNA proved to be significantly higher in carriers of the rare alleles (P=0.039) for both genetic variants. ConclusionOur exploratory results suggest that while rs2274273 and rs17128183 bear no association with the risk of advanced CA or CA-related complications, these genetic variants are likely to affect LGALS-3 expression levels. In order to reach a definitive conclusion on the role played by rs2274273 and rs17128183 in advanced CA, our results should be further validated.
T2  - Journal of Clinical Laboratory Analysis
T1  - Genetic Variants in the Vicinity of LGALS-3 Gene and LGALS-3 mRNA Expression in Advanced Carotid Atherosclerosis: An Exploratory Study
VL  - 30
IS  - 6
SP  - 1150
EP  - 1157
DO  - 10.1002/jcla.21996
ER  - 
@article{
author = "Đorđević, Ana D. and Živković, Maja and Stanković, Aleksandra and Životić, Ivan and Končar, Igor and Davidović, Lazar and Alavantić, Dragan and Đurić, Tamara",
year = "2016",
abstract = "BackgroundPrevious research has shown that there is an association between galectin-3 (gal-3) protein and cardiovascular pathology. The aim of this study was to investigate the effects of rs2274273 and rs17128183 on genetic susceptibility to advanced carotid atherosclerosis (CA) and its complications. The rs2274273 has been singled out as the lead SNP of the haplotype block containing LGALS-3 (gal-3 gene) associated with gal-3 circulating levels, while rs17128183 constitutes a potentially functional SNP of the same hap-block. We further sought to determine whether these genetic variants have an impact on the expression of LGALS-3 mRNA in human carotid atherosclerotic plaque tissue. MethodsThe study encompassed 300 control subjects and 485 patients with advanced CA who had undergone carotid endarterectomy. Rs2274273, rs17128183, and LGALS-3 relative mRNA expression was detected by means of real-time PCR (TaqMan((R)) technology). ResultsThere were no statistically significant associations of the investigated genetic variants with susceptibility to advanced CA, nor did we find any associations in terms of ultrasonographically defined plaque phenotypes. The relative expression of LGALS-3 mRNA proved to be significantly higher in carriers of the rare alleles (P=0.039) for both genetic variants. ConclusionOur exploratory results suggest that while rs2274273 and rs17128183 bear no association with the risk of advanced CA or CA-related complications, these genetic variants are likely to affect LGALS-3 expression levels. In order to reach a definitive conclusion on the role played by rs2274273 and rs17128183 in advanced CA, our results should be further validated.",
journal = "Journal of Clinical Laboratory Analysis",
title = "Genetic Variants in the Vicinity of LGALS-3 Gene and LGALS-3 mRNA Expression in Advanced Carotid Atherosclerosis: An Exploratory Study",
volume = "30",
number = "6",
pages = "1150-1157",
doi = "10.1002/jcla.21996"
}
Đorđević, A. D., Živković, M., Stanković, A., Životić, I., Končar, I., Davidović, L., Alavantić, D.,& Đurić, T.. (2016). Genetic Variants in the Vicinity of LGALS-3 Gene and LGALS-3 mRNA Expression in Advanced Carotid Atherosclerosis: An Exploratory Study. in Journal of Clinical Laboratory Analysis, 30(6), 1150-1157.
https://doi.org/10.1002/jcla.21996
Đorđević AD, Živković M, Stanković A, Životić I, Končar I, Davidović L, Alavantić D, Đurić T. Genetic Variants in the Vicinity of LGALS-3 Gene and LGALS-3 mRNA Expression in Advanced Carotid Atherosclerosis: An Exploratory Study. in Journal of Clinical Laboratory Analysis. 2016;30(6):1150-1157.
doi:10.1002/jcla.21996 .
Đorđević, Ana D., Živković, Maja, Stanković, Aleksandra, Životić, Ivan, Končar, Igor, Davidović, Lazar, Alavantić, Dragan, Đurić, Tamara, "Genetic Variants in the Vicinity of LGALS-3 Gene and LGALS-3 mRNA Expression in Advanced Carotid Atherosclerosis: An Exploratory Study" in Journal of Clinical Laboratory Analysis, 30, no. 6 (2016):1150-1157,
https://doi.org/10.1002/jcla.21996 . .
9
8
8

Transcriptome-wide based identification of miRs in congenital anomalies of the kidney and urinary tract (CAKUT) in children: the significant upregulation of tissue miR-144 expression

Jovanović, Ivan G.; Živković, Maja; Kostić, Mirjana M.; Krstić, Zoran; Đurić, Tamara; Kolić, Ivana; Alavantić, Dragan; Stanković, Aleksandra

(2016)

TY  - JOUR
AU  - Jovanović, Ivan G.
AU  - Živković, Maja
AU  - Kostić, Mirjana M.
AU  - Krstić, Zoran
AU  - Đurić, Tamara
AU  - Kolić, Ivana
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1182
AB  - Background: The genetic cause of most congenital anomalies of the kidney and urinary tract (CAKUT) cases remains unknown, therefore the novel approaches in searching for the common disease denominators are required. miRs regulate gene expression in humans and therefore have potentially therapeutic and biomarker properties. No studies thus far have attempted to explore the miRs in human CAKUT. We applied a new strategy to identify most specific miRs associated with CAKUT, in pediatric patients. Methods: Data from the whole genome expression, gathered from ureter tissue samples of 19 patients and 7 controls, were used for the bioinformatic prediction of miRs activity in CAKUT. We integrated microarray gene expression data and miR target predictions from multiple prediction algorithms using Co-inertia analysis (CIA) in conjunction with correspondence analysis and between group analysis, to produce a ranked list of miRs associated with CAKUT. The CIA included five different sequence based miR target prediction algorithms and the Co-expression Meta-analysis of miR Targets. For the experimental validation of expression of miRs identified by the CIA we used tissue from 36 CAKUT patients and 9 controls. The results of gene ontology (GO) analysis on co-expressed targets of miRs associated with CAKUT were used for the selection of putative biological processes relevant to CAKUT. Results: We identified 7 miRs with a potential role in CAKUT. The top ranked miRs from miRCos communities 4, 1 and 7 were chosen for experimental validation of expression in CAKUT tissue. The 5.7 fold increase of hsa-miR-144 expression in human tissue from CAKUT patients compared to controls (p = 0.005) was observed. From the GO we selected 7 biological processes that could contribute to CAKUT, which genes are potentially influenced by hsa-miR-144. The hsa-miR-200a, hsa-miR-183 and hsa-miR-375 werent differentially expressed in CAKUT. Conclusions: This study shows that integrative approach applied here was useful in identification of the miRs associated with CAKUT. The hsa-miR-144, first time identified in CAKUT, could be connected with biological processes crucial for normal development of kidney and urinary tract. Further functional analysis must follow to reveal the impact of hsa-miR-144 on CAKUT occurrence.
T2  - Journal of Translational Medicine
T1  - Transcriptome-wide based identification of miRs in congenital anomalies of the kidney and urinary tract (CAKUT) in children: the significant upregulation of tissue miR-144 expression
VL  - 14
IS  - 1
SP  - 193
DO  - 10.1186/s12967-016-0955-0
ER  - 
@article{
author = "Jovanović, Ivan G. and Živković, Maja and Kostić, Mirjana M. and Krstić, Zoran and Đurić, Tamara and Kolić, Ivana and Alavantić, Dragan and Stanković, Aleksandra",
year = "2016",
abstract = "Background: The genetic cause of most congenital anomalies of the kidney and urinary tract (CAKUT) cases remains unknown, therefore the novel approaches in searching for the common disease denominators are required. miRs regulate gene expression in humans and therefore have potentially therapeutic and biomarker properties. No studies thus far have attempted to explore the miRs in human CAKUT. We applied a new strategy to identify most specific miRs associated with CAKUT, in pediatric patients. Methods: Data from the whole genome expression, gathered from ureter tissue samples of 19 patients and 7 controls, were used for the bioinformatic prediction of miRs activity in CAKUT. We integrated microarray gene expression data and miR target predictions from multiple prediction algorithms using Co-inertia analysis (CIA) in conjunction with correspondence analysis and between group analysis, to produce a ranked list of miRs associated with CAKUT. The CIA included five different sequence based miR target prediction algorithms and the Co-expression Meta-analysis of miR Targets. For the experimental validation of expression of miRs identified by the CIA we used tissue from 36 CAKUT patients and 9 controls. The results of gene ontology (GO) analysis on co-expressed targets of miRs associated with CAKUT were used for the selection of putative biological processes relevant to CAKUT. Results: We identified 7 miRs with a potential role in CAKUT. The top ranked miRs from miRCos communities 4, 1 and 7 were chosen for experimental validation of expression in CAKUT tissue. The 5.7 fold increase of hsa-miR-144 expression in human tissue from CAKUT patients compared to controls (p = 0.005) was observed. From the GO we selected 7 biological processes that could contribute to CAKUT, which genes are potentially influenced by hsa-miR-144. The hsa-miR-200a, hsa-miR-183 and hsa-miR-375 werent differentially expressed in CAKUT. Conclusions: This study shows that integrative approach applied here was useful in identification of the miRs associated with CAKUT. The hsa-miR-144, first time identified in CAKUT, could be connected with biological processes crucial for normal development of kidney and urinary tract. Further functional analysis must follow to reveal the impact of hsa-miR-144 on CAKUT occurrence.",
journal = "Journal of Translational Medicine",
title = "Transcriptome-wide based identification of miRs in congenital anomalies of the kidney and urinary tract (CAKUT) in children: the significant upregulation of tissue miR-144 expression",
volume = "14",
number = "1",
pages = "193",
doi = "10.1186/s12967-016-0955-0"
}
Jovanović, I. G., Živković, M., Kostić, M. M., Krstić, Z., Đurić, T., Kolić, I., Alavantić, D.,& Stanković, A.. (2016). Transcriptome-wide based identification of miRs in congenital anomalies of the kidney and urinary tract (CAKUT) in children: the significant upregulation of tissue miR-144 expression. in Journal of Translational Medicine, 14(1), 193.
https://doi.org/10.1186/s12967-016-0955-0
Jovanović IG, Živković M, Kostić MM, Krstić Z, Đurić T, Kolić I, Alavantić D, Stanković A. Transcriptome-wide based identification of miRs in congenital anomalies of the kidney and urinary tract (CAKUT) in children: the significant upregulation of tissue miR-144 expression. in Journal of Translational Medicine. 2016;14(1):193.
doi:10.1186/s12967-016-0955-0 .
Jovanović, Ivan G., Živković, Maja, Kostić, Mirjana M., Krstić, Zoran, Đurić, Tamara, Kolić, Ivana, Alavantić, Dragan, Stanković, Aleksandra, "Transcriptome-wide based identification of miRs in congenital anomalies of the kidney and urinary tract (CAKUT) in children: the significant upregulation of tissue miR-144 expression" in Journal of Translational Medicine, 14, no. 1 (2016):193,
https://doi.org/10.1186/s12967-016-0955-0 . .
2
9
7
7

9p21 locus rs10757278 is associated with advanced carotid atherosclerosis in a gender-specific manner

Životić, Ivan; Đurić, Tamara; Stanković, Aleksandra; Đorđević, Ana D.; Končar, Igor; Davidović, Lazar; Alavantić, Dragan; Živković, Maja

(2016)

TY  - JOUR
AU  - Životić, Ivan
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Đorđević, Ana D.
AU  - Končar, Igor
AU  - Davidović, Lazar
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1126
AB  - Single nucleotide polymorphisms from the chromosome locus 9p21 are reported to carry a risk for various cardiovascular diseases. One of the lead single nucleotide polymorphisms, rs10757278, was mostly investigated in association with coronary artery disease but rarely with carotid atherosclerosis. In this study, we aimed to analyze the association of rs10757278 A/G polymorphism with carotid plaque presence in advanced carotid atherosclerosis. The study included 803 participants, 486 patients with high-grade stenosis ( GT 70%) who were undergoing carotid endarterectomy and 317 controls from Serbian population. Genotypes were determined using the real-time polymerase chain reaction. According to the recessive model of inheritance, GG genotype was significantly and independently associated with carotid plaque in females only (odds ratio 2.42, CI = 1.20-4.90, P = 0.013). Odds ratio was adjusted for age, body mass index, hypertension, TC, LDLC, HDLC and TG, and P value was corrected for multiple comparisons. Our preliminary findings suggest a gender-specific association of rs10757278 polymorphism with carotid plaque. Further studies on larger sample and in genetically and environmentally similar populations are needed.
T2  - Experimental Biology and Medicine
T1  - 9p21 locus rs10757278 is associated with advanced carotid atherosclerosis in a gender-specific manner
VL  - 241
IS  - 11
SP  - 1210
EP  - 1216
DO  - 10.1177/1535370216636718
ER  - 
@article{
author = "Životić, Ivan and Đurić, Tamara and Stanković, Aleksandra and Đorđević, Ana D. and Končar, Igor and Davidović, Lazar and Alavantić, Dragan and Živković, Maja",
year = "2016",
abstract = "Single nucleotide polymorphisms from the chromosome locus 9p21 are reported to carry a risk for various cardiovascular diseases. One of the lead single nucleotide polymorphisms, rs10757278, was mostly investigated in association with coronary artery disease but rarely with carotid atherosclerosis. In this study, we aimed to analyze the association of rs10757278 A/G polymorphism with carotid plaque presence in advanced carotid atherosclerosis. The study included 803 participants, 486 patients with high-grade stenosis ( GT 70%) who were undergoing carotid endarterectomy and 317 controls from Serbian population. Genotypes were determined using the real-time polymerase chain reaction. According to the recessive model of inheritance, GG genotype was significantly and independently associated with carotid plaque in females only (odds ratio 2.42, CI = 1.20-4.90, P = 0.013). Odds ratio was adjusted for age, body mass index, hypertension, TC, LDLC, HDLC and TG, and P value was corrected for multiple comparisons. Our preliminary findings suggest a gender-specific association of rs10757278 polymorphism with carotid plaque. Further studies on larger sample and in genetically and environmentally similar populations are needed.",
journal = "Experimental Biology and Medicine",
title = "9p21 locus rs10757278 is associated with advanced carotid atherosclerosis in a gender-specific manner",
volume = "241",
number = "11",
pages = "1210-1216",
doi = "10.1177/1535370216636718"
}
Životić, I., Đurić, T., Stanković, A., Đorđević, A. D., Končar, I., Davidović, L., Alavantić, D.,& Živković, M.. (2016). 9p21 locus rs10757278 is associated with advanced carotid atherosclerosis in a gender-specific manner. in Experimental Biology and Medicine, 241(11), 1210-1216.
https://doi.org/10.1177/1535370216636718
Životić I, Đurić T, Stanković A, Đorđević AD, Končar I, Davidović L, Alavantić D, Živković M. 9p21 locus rs10757278 is associated with advanced carotid atherosclerosis in a gender-specific manner. in Experimental Biology and Medicine. 2016;241(11):1210-1216.
doi:10.1177/1535370216636718 .
Životić, Ivan, Đurić, Tamara, Stanković, Aleksandra, Đorđević, Ana D., Končar, Igor, Davidović, Lazar, Alavantić, Dragan, Živković, Maja, "9p21 locus rs10757278 is associated with advanced carotid atherosclerosis in a gender-specific manner" in Experimental Biology and Medicine, 241, no. 11 (2016):1210-1216,
https://doi.org/10.1177/1535370216636718 . .
1
3
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5

Rs2274273 from LGALS-3 locus and cardiac ventricular remodeling after first myocardial infarction: preliminary results

Đorđević, Ana D.; Đurić, Tamara; Živković, Maja; Životić, Ivan; Dekleva, Milica; Marković-Nikolić, Nataša; Alavantić, Dragan; Stanković, Aleksandra

(2016)

TY  - CONF
AU  - Đorđević, Ana D.
AU  - Đurić, Tamara
AU  - Živković, Maja
AU  - Životić, Ivan
AU  - Dekleva, Milica
AU  - Marković-Nikolić, Nataša
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1101
C3  - European Journal of Heart Failure
T1  - Rs2274273 from LGALS-3 locus and cardiac ventricular remodeling after first myocardial infarction: preliminary results
VL  - 18
IS  - SI
SP  - 283
EP  - 284
ER  - 
@conference{
author = "Đorđević, Ana D. and Đurić, Tamara and Živković, Maja and Životić, Ivan and Dekleva, Milica and Marković-Nikolić, Nataša and Alavantić, Dragan and Stanković, Aleksandra",
year = "2016",
journal = "European Journal of Heart Failure",
title = "Rs2274273 from LGALS-3 locus and cardiac ventricular remodeling after first myocardial infarction: preliminary results",
volume = "18",
number = "SI",
pages = "283-284"
}
Đorđević, A. D., Đurić, T., Živković, M., Životić, I., Dekleva, M., Marković-Nikolić, N., Alavantić, D.,& Stanković, A.. (2016). Rs2274273 from LGALS-3 locus and cardiac ventricular remodeling after first myocardial infarction: preliminary results. in European Journal of Heart Failure, 18(SI), 283-284.
Đorđević AD, Đurić T, Živković M, Životić I, Dekleva M, Marković-Nikolić N, Alavantić D, Stanković A. Rs2274273 from LGALS-3 locus and cardiac ventricular remodeling after first myocardial infarction: preliminary results. in European Journal of Heart Failure. 2016;18(SI):283-284..
Đorđević, Ana D., Đurić, Tamara, Živković, Maja, Životić, Ivan, Dekleva, Milica, Marković-Nikolić, Nataša, Alavantić, Dragan, Stanković, Aleksandra, "Rs2274273 from LGALS-3 locus and cardiac ventricular remodeling after first myocardial infarction: preliminary results" in European Journal of Heart Failure, 18, no. SI (2016):283-284.

Gender-Specific Association between Angiotensin II Type 2 Receptor-1332 A/G Gene Polymorphism and Advanced Carotid Atherosclerosis

Kolaković, Ana; Stanković, Aleksandra; Đurić, Tamara; Živković, Maja; Končar, Igor; Davidović, Lazar; Radak, Đorđe J.; Alavantić, Dragan

(2016)

TY  - JOUR
AU  - Kolaković, Ana
AU  - Stanković, Aleksandra
AU  - Đurić, Tamara
AU  - Živković, Maja
AU  - Končar, Igor
AU  - Davidović, Lazar
AU  - Radak, Đorđe J.
AU  - Alavantić, Dragan
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1123
AB  - Background: The angiotensin II type 2 receptor (AT2R) - 1332 A/G polymorphism has been denoted as functional and associated with certain cardiovascular disease phenotypes. However, there are no studies considering the association of this gene polymorphism with carotid atherosclerosis (CA) and cerebrovascular events. Therefore, the aim of our study was to investigate a possible association of the AT2R - 1332 A/G polymorphism with the occurrence of carotid plaques (CPs) and history of cerebrovascular insult (CVI) in advanced CA. Methods: The study group included 381 controls and 509 patients with CA consecutively admitted for endarterectomy. Genotyping was determined by polymerase chain reaction-restriction fragment length polymorphism method. The association was analyzed separately for males and females because the AT2R gene is located on the X chromosome. Results: The AT2R - 1332 GG genotype was associated with the advanced CA in the female study group (recessive model of inheritance, AA+AG versus GG; adjusted odds ratio [OR] = 2.25; 95% confidence interval [CI] 1.17-4.33; P=.01). In the male subgroup of patients with CA, the significant overrepresentation of G/- hemizygote was detected in patients with CVI compared to male patients without this event (crude OR = 2.05, 95% CI 1.20-3.50, P=.008). Conclusions: This study suggests a gender-specific association between the AT2R -1332 A/G polymorphism and the occurrence of CP and the history of CVI in advanced CA, but further replication studies are needed. (C) 2016 National Stroke Association. Published by Elsevier Inc. All rights reserved.
T2  - Journal of Stroke and Cerebrovascular Diseases
T1  - Gender-Specific Association between Angiotensin II Type 2 Receptor-1332 A/G Gene Polymorphism and Advanced Carotid Atherosclerosis
VL  - 25
IS  - 7
SP  - 1622
EP  - 1630
DO  - 10.1016/j.jstrokecerebrovasdis.2016.03.011
ER  - 
@article{
author = "Kolaković, Ana and Stanković, Aleksandra and Đurić, Tamara and Živković, Maja and Končar, Igor and Davidović, Lazar and Radak, Đorđe J. and Alavantić, Dragan",
year = "2016",
abstract = "Background: The angiotensin II type 2 receptor (AT2R) - 1332 A/G polymorphism has been denoted as functional and associated with certain cardiovascular disease phenotypes. However, there are no studies considering the association of this gene polymorphism with carotid atherosclerosis (CA) and cerebrovascular events. Therefore, the aim of our study was to investigate a possible association of the AT2R - 1332 A/G polymorphism with the occurrence of carotid plaques (CPs) and history of cerebrovascular insult (CVI) in advanced CA. Methods: The study group included 381 controls and 509 patients with CA consecutively admitted for endarterectomy. Genotyping was determined by polymerase chain reaction-restriction fragment length polymorphism method. The association was analyzed separately for males and females because the AT2R gene is located on the X chromosome. Results: The AT2R - 1332 GG genotype was associated with the advanced CA in the female study group (recessive model of inheritance, AA+AG versus GG; adjusted odds ratio [OR] = 2.25; 95% confidence interval [CI] 1.17-4.33; P=.01). In the male subgroup of patients with CA, the significant overrepresentation of G/- hemizygote was detected in patients with CVI compared to male patients without this event (crude OR = 2.05, 95% CI 1.20-3.50, P=.008). Conclusions: This study suggests a gender-specific association between the AT2R -1332 A/G polymorphism and the occurrence of CP and the history of CVI in advanced CA, but further replication studies are needed. (C) 2016 National Stroke Association. Published by Elsevier Inc. All rights reserved.",
journal = "Journal of Stroke and Cerebrovascular Diseases",
title = "Gender-Specific Association between Angiotensin II Type 2 Receptor-1332 A/G Gene Polymorphism and Advanced Carotid Atherosclerosis",
volume = "25",
number = "7",
pages = "1622-1630",
doi = "10.1016/j.jstrokecerebrovasdis.2016.03.011"
}
Kolaković, A., Stanković, A., Đurić, T., Živković, M., Končar, I., Davidović, L., Radak, Đ. J.,& Alavantić, D.. (2016). Gender-Specific Association between Angiotensin II Type 2 Receptor-1332 A/G Gene Polymorphism and Advanced Carotid Atherosclerosis. in Journal of Stroke and Cerebrovascular Diseases, 25(7), 1622-1630.
https://doi.org/10.1016/j.jstrokecerebrovasdis.2016.03.011
Kolaković A, Stanković A, Đurić T, Živković M, Končar I, Davidović L, Radak ĐJ, Alavantić D. Gender-Specific Association between Angiotensin II Type 2 Receptor-1332 A/G Gene Polymorphism and Advanced Carotid Atherosclerosis. in Journal of Stroke and Cerebrovascular Diseases. 2016;25(7):1622-1630.
doi:10.1016/j.jstrokecerebrovasdis.2016.03.011 .
Kolaković, Ana, Stanković, Aleksandra, Đurić, Tamara, Živković, Maja, Končar, Igor, Davidović, Lazar, Radak, Đorđe J., Alavantić, Dragan, "Gender-Specific Association between Angiotensin II Type 2 Receptor-1332 A/G Gene Polymorphism and Advanced Carotid Atherosclerosis" in Journal of Stroke and Cerebrovascular Diseases, 25, no. 7 (2016):1622-1630,
https://doi.org/10.1016/j.jstrokecerebrovasdis.2016.03.011 . .
2
5
4
4

Fructose-rich diet induces gender-specific changes in expression of the renin-angiotensin system in rat heart and upregulates the ACE/AT1R axis in the male rat aorta

Bundalo, Maja M.; Živković, Maja; Romić, Snježana Đ.; Tepavčević, Snežana; Korićanac, Goran; Đurić, Tamara; Stanković, Aleksandra

(2016)

TY  - JOUR
AU  - Bundalo, Maja M.
AU  - Živković, Maja
AU  - Romić, Snježana Đ.
AU  - Tepavčević, Snežana
AU  - Korićanac, Goran
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1132
AB  - Introduction: The cardiovascular renin-angiotensin system (RAS) could be affected by gender and dietary regime. We hypothesized that male rats will be more susceptible to activation of RAS in the heart and aorta, as a response to a fructose-rich diet (FRD). Materials and methods: Both male and female Wistar rats were given a 10% (w/v) fructose solution for 9 weeks. We measured the biochemical parameters, blood pressure (BP) and heart rate. We used Western blot and real-time polymerase chain reaction (PCR) to quantify protein and gene expression. Results: In the male rats, the FRD elevated BP and expression of cardiac angiotensin-converting enzyme (ACE), while the expression of angiotensin-converting enzyme 2 (ACE2) and angiotensin II Type 2 receptor (AT(2)R) were significantly decreased. In female rats, there were no changes in cardiac RAS expression due to FRD. Furthermore, the ACE/AT(1)R axis was overexpressed in the FRD male rats aortae, while only AT(1)R was upregulated in the FRD female rats aortae. ACE2 expression remained unchanged in the aortae of both genders receiving the FRD. Conclusions: The FRD induced gender-specific changes in the expression of the RAS in the heart and aortae of male rats. Further investigations are required in order to get a comprehensive understanding of the underlying mechanisms of gender-specific fructose-induced cardiovascular pathologies.
T2  - Journal of the Renin-Angiotensin-Aldosterone System
T1  - Fructose-rich diet induces gender-specific changes in expression of the renin-angiotensin system in rat heart and upregulates the ACE/AT1R axis in the male rat aorta
VL  - 17
IS  - 2
DO  - 10.1177/1470320316642915
ER  - 
@article{
author = "Bundalo, Maja M. and Živković, Maja and Romić, Snježana Đ. and Tepavčević, Snežana and Korićanac, Goran and Đurić, Tamara and Stanković, Aleksandra",
year = "2016",
abstract = "Introduction: The cardiovascular renin-angiotensin system (RAS) could be affected by gender and dietary regime. We hypothesized that male rats will be more susceptible to activation of RAS in the heart and aorta, as a response to a fructose-rich diet (FRD). Materials and methods: Both male and female Wistar rats were given a 10% (w/v) fructose solution for 9 weeks. We measured the biochemical parameters, blood pressure (BP) and heart rate. We used Western blot and real-time polymerase chain reaction (PCR) to quantify protein and gene expression. Results: In the male rats, the FRD elevated BP and expression of cardiac angiotensin-converting enzyme (ACE), while the expression of angiotensin-converting enzyme 2 (ACE2) and angiotensin II Type 2 receptor (AT(2)R) were significantly decreased. In female rats, there were no changes in cardiac RAS expression due to FRD. Furthermore, the ACE/AT(1)R axis was overexpressed in the FRD male rats aortae, while only AT(1)R was upregulated in the FRD female rats aortae. ACE2 expression remained unchanged in the aortae of both genders receiving the FRD. Conclusions: The FRD induced gender-specific changes in the expression of the RAS in the heart and aortae of male rats. Further investigations are required in order to get a comprehensive understanding of the underlying mechanisms of gender-specific fructose-induced cardiovascular pathologies.",
journal = "Journal of the Renin-Angiotensin-Aldosterone System",
title = "Fructose-rich diet induces gender-specific changes in expression of the renin-angiotensin system in rat heart and upregulates the ACE/AT1R axis in the male rat aorta",
volume = "17",
number = "2",
doi = "10.1177/1470320316642915"
}
Bundalo, M. M., Živković, M., Romić, S. Đ., Tepavčević, S., Korićanac, G., Đurić, T.,& Stanković, A.. (2016). Fructose-rich diet induces gender-specific changes in expression of the renin-angiotensin system in rat heart and upregulates the ACE/AT1R axis in the male rat aorta. in Journal of the Renin-Angiotensin-Aldosterone System, 17(2).
https://doi.org/10.1177/1470320316642915
Bundalo MM, Živković M, Romić SĐ, Tepavčević S, Korićanac G, Đurić T, Stanković A. Fructose-rich diet induces gender-specific changes in expression of the renin-angiotensin system in rat heart and upregulates the ACE/AT1R axis in the male rat aorta. in Journal of the Renin-Angiotensin-Aldosterone System. 2016;17(2).
doi:10.1177/1470320316642915 .
Bundalo, Maja M., Živković, Maja, Romić, Snježana Đ., Tepavčević, Snežana, Korićanac, Goran, Đurić, Tamara, Stanković, Aleksandra, "Fructose-rich diet induces gender-specific changes in expression of the renin-angiotensin system in rat heart and upregulates the ACE/AT1R axis in the male rat aorta" in Journal of the Renin-Angiotensin-Aldosterone System, 17, no. 2 (2016),
https://doi.org/10.1177/1470320316642915 . .
19
17
18

Angiotensin receptor type 1 polymorphism A1166C is associated with altered AT1R and miR-155 expression in carotid plaque tissue and development of hypoechoic carotid plaques

Stanković, Aleksandra; Kolaković, Ana; Živković, Maja; Đurić, Tamara; Bundalo, Maja M.; Končar, Igor; Davidović, Lazar; Alavantić, Dragan

(Elsevier, 2016)

TY  - JOUR
AU  - Stanković, Aleksandra
AU  - Kolaković, Ana
AU  - Živković, Maja
AU  - Đurić, Tamara
AU  - Bundalo, Maja M.
AU  - Končar, Igor
AU  - Davidović, Lazar
AU  - Alavantić, Dragan
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1045
AB  - Background and Aims: The principal biologic effects of the renin-angiotensin system are mediated by activation of the AT1R receptor. The microRNA miR-155 regulates AT1R expression, with both its, and AT1Rs activity, linked to atherosclerosis. Target sites for miR-155 lie within the 3 UTR of the human AT1R gene, and include the AT1R A1166C polymorphism. Thus far, only levels of circulating miR-155 have been investigated with respect to A1166C genotypes. We hypothesized that the A1166C polymorphism could correlate with different, ultra-sonographically defined plaque phenotypes, as well as with an altered expression of AT1R mRNA and protein in human carotid plaques (CP), and altered expression of miR-155 in patients with advanced atherosclerosis. Methods: Our study cohort comprised 411 patients with advanced carotid atherosclerosis (298 hyperechoic; 113 hypoechoic plaques). PCR analyses identified A1166C genotypes; quantitative real-time PCR determined AT1R and miR-155 expression levels, with AT1R protein expression evaluated by western blot. Results: Genotypes containing the C allele bore a significant association with the hypoechoic plaque phenotype (adjusted OR 1.87, 95% CI 1.16-3.00, p = 0.01). The expression of AT1R mRNA and miR-155 were significantly up-regulated in the CPs of CC genotype carriers compared to the AA/AC genotypes (p = 0.032, p = 0.015, respectively). AT1R protein expression was also significantly higher for CC genotypes (p LT 0.01). Conclusion: Our results indicate that the AT1R A1166C polymorphism impacts an ultrasonographicallydefined human plaque phenotype, with intra-plaque AT1R and miR-155 expression altered in advanced carotid atherosclerosis. Validation and replication of these data should contribute to an improved personalized therapy with which to prevent carotid atherosclerosis. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
PB  - Elsevier
T2  - Atherosclerosis
T1  - Angiotensin receptor type 1 polymorphism A1166C is associated with altered AT1R and miR-155 expression in carotid plaque tissue and development of hypoechoic carotid plaques
VL  - 248
SP  - 132
EP  - 139
DO  - 10.1016/j.atherosclerosis.2016.02.032
ER  - 
@article{
author = "Stanković, Aleksandra and Kolaković, Ana and Živković, Maja and Đurić, Tamara and Bundalo, Maja M. and Končar, Igor and Davidović, Lazar and Alavantić, Dragan",
year = "2016",
abstract = "Background and Aims: The principal biologic effects of the renin-angiotensin system are mediated by activation of the AT1R receptor. The microRNA miR-155 regulates AT1R expression, with both its, and AT1Rs activity, linked to atherosclerosis. Target sites for miR-155 lie within the 3 UTR of the human AT1R gene, and include the AT1R A1166C polymorphism. Thus far, only levels of circulating miR-155 have been investigated with respect to A1166C genotypes. We hypothesized that the A1166C polymorphism could correlate with different, ultra-sonographically defined plaque phenotypes, as well as with an altered expression of AT1R mRNA and protein in human carotid plaques (CP), and altered expression of miR-155 in patients with advanced atherosclerosis. Methods: Our study cohort comprised 411 patients with advanced carotid atherosclerosis (298 hyperechoic; 113 hypoechoic plaques). PCR analyses identified A1166C genotypes; quantitative real-time PCR determined AT1R and miR-155 expression levels, with AT1R protein expression evaluated by western blot. Results: Genotypes containing the C allele bore a significant association with the hypoechoic plaque phenotype (adjusted OR 1.87, 95% CI 1.16-3.00, p = 0.01). The expression of AT1R mRNA and miR-155 were significantly up-regulated in the CPs of CC genotype carriers compared to the AA/AC genotypes (p = 0.032, p = 0.015, respectively). AT1R protein expression was also significantly higher for CC genotypes (p LT 0.01). Conclusion: Our results indicate that the AT1R A1166C polymorphism impacts an ultrasonographicallydefined human plaque phenotype, with intra-plaque AT1R and miR-155 expression altered in advanced carotid atherosclerosis. Validation and replication of these data should contribute to an improved personalized therapy with which to prevent carotid atherosclerosis. (C) 2016 Elsevier Ireland Ltd. All rights reserved.",
publisher = "Elsevier",
journal = "Atherosclerosis",
title = "Angiotensin receptor type 1 polymorphism A1166C is associated with altered AT1R and miR-155 expression in carotid plaque tissue and development of hypoechoic carotid plaques",
volume = "248",
pages = "132-139",
doi = "10.1016/j.atherosclerosis.2016.02.032"
}
Stanković, A., Kolaković, A., Živković, M., Đurić, T., Bundalo, M. M., Končar, I., Davidović, L.,& Alavantić, D.. (2016). Angiotensin receptor type 1 polymorphism A1166C is associated with altered AT1R and miR-155 expression in carotid plaque tissue and development of hypoechoic carotid plaques. in Atherosclerosis
Elsevier., 248, 132-139.
https://doi.org/10.1016/j.atherosclerosis.2016.02.032
Stanković A, Kolaković A, Živković M, Đurić T, Bundalo MM, Končar I, Davidović L, Alavantić D. Angiotensin receptor type 1 polymorphism A1166C is associated with altered AT1R and miR-155 expression in carotid plaque tissue and development of hypoechoic carotid plaques. in Atherosclerosis. 2016;248:132-139.
doi:10.1016/j.atherosclerosis.2016.02.032 .
Stanković, Aleksandra, Kolaković, Ana, Živković, Maja, Đurić, Tamara, Bundalo, Maja M., Končar, Igor, Davidović, Lazar, Alavantić, Dragan, "Angiotensin receptor type 1 polymorphism A1166C is associated with altered AT1R and miR-155 expression in carotid plaque tissue and development of hypoechoic carotid plaques" in Atherosclerosis, 248 (2016):132-139,
https://doi.org/10.1016/j.atherosclerosis.2016.02.032 . .
1
9
5
7

CXCL16 Haplotypes in Patients with Human Carotid Atherosclerosis: Preliminary Results

Živković, Maja; Đurić, Tamara; Stojković, Ljiljana S.; Jovanović, Ivan G.; Končar, Igor; Davidović, Lazar; Veljković, Nevena V.; Alavantić, Dragan; Stanković, Aleksandra

(2015)

TY  - JOUR
AU  - Živković, Maja
AU  - Đurić, Tamara
AU  - Stojković, Ljiljana S.
AU  - Jovanović, Ivan G.
AU  - Končar, Igor
AU  - Davidović, Lazar
AU  - Veljković, Nevena V.
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/481
AB  - Aim: Chemokine CXC ligand 16 (CXCL16) has chemoattractive, adhesive and scavenging properties and may play a role in the formation of atherosclerotic lesions. However, studies of CXCL16 polymorphisms in patients with atherosclerosis are scarce. The missense polymorphisms I123T and A181V are potentially important factors in the regulation of presentation and shedding of the CXCL16 chemokine domain. The aim of this study was to analyze the association between I123T and A181V polymorphism haplotypes and the accumulation of carotid plaque as well as the effect of the haplotype on the CXCL16 mRNA expression in carotid plaques in patients with advanced atherosclerosis. Additionally, we performed a bioinformatic prediction analysis of the impact of CXCL16 protein sequence variation on CXCL16-CXCR6 interactions and analyzed the soluble CXCL16 plasma levels according to the CXCL16 haplotype. Methods: This study evaluated a total of 733 participants, including 283 controls and 450 patients with carotid atherosclerosis (CA) undergoing endarterectomy. Analyses of the polymorphisms and the gene expression were performed using real-time PCR. The soluble CXCL16 levels were measured with ELISA. Results: The missense allele haplotype, T123V181, was found to be significantly and independently associated with the occurrence of CA plaque (OR=1.27; 1.02-1.57, p=0.03). This haplotype was predicted to significantly change the CXCL16-CXCR6 interaction, compared to I123A181. Neither the CXCL16 mRNA expression in the human plaques nor the soluble CXCL16 plasma levels differed according to the haplotype. Conclusions: These results indicate that the CXCL16 T123V181 haplotype is a moderate genetic risk factor for the development of carotid plaque. Further functional and replication studies are needed to clarify the mechanisms by which this combination of alleles promotes advanced CA and validate its impact on disease progression.
T2  - Journal of Atherosclerosis and Thrombosis
T1  - CXCL16 Haplotypes in Patients with Human Carotid Atherosclerosis: Preliminary Results
VL  - 22
IS  - 1
SP  - 10
EP  - 20
DO  - 10.5551/jat.24299
ER  - 
@article{
author = "Živković, Maja and Đurić, Tamara and Stojković, Ljiljana S. and Jovanović, Ivan G. and Končar, Igor and Davidović, Lazar and Veljković, Nevena V. and Alavantić, Dragan and Stanković, Aleksandra",
year = "2015",
abstract = "Aim: Chemokine CXC ligand 16 (CXCL16) has chemoattractive, adhesive and scavenging properties and may play a role in the formation of atherosclerotic lesions. However, studies of CXCL16 polymorphisms in patients with atherosclerosis are scarce. The missense polymorphisms I123T and A181V are potentially important factors in the regulation of presentation and shedding of the CXCL16 chemokine domain. The aim of this study was to analyze the association between I123T and A181V polymorphism haplotypes and the accumulation of carotid plaque as well as the effect of the haplotype on the CXCL16 mRNA expression in carotid plaques in patients with advanced atherosclerosis. Additionally, we performed a bioinformatic prediction analysis of the impact of CXCL16 protein sequence variation on CXCL16-CXCR6 interactions and analyzed the soluble CXCL16 plasma levels according to the CXCL16 haplotype. Methods: This study evaluated a total of 733 participants, including 283 controls and 450 patients with carotid atherosclerosis (CA) undergoing endarterectomy. Analyses of the polymorphisms and the gene expression were performed using real-time PCR. The soluble CXCL16 levels were measured with ELISA. Results: The missense allele haplotype, T123V181, was found to be significantly and independently associated with the occurrence of CA plaque (OR=1.27; 1.02-1.57, p=0.03). This haplotype was predicted to significantly change the CXCL16-CXCR6 interaction, compared to I123A181. Neither the CXCL16 mRNA expression in the human plaques nor the soluble CXCL16 plasma levels differed according to the haplotype. Conclusions: These results indicate that the CXCL16 T123V181 haplotype is a moderate genetic risk factor for the development of carotid plaque. Further functional and replication studies are needed to clarify the mechanisms by which this combination of alleles promotes advanced CA and validate its impact on disease progression.",
journal = "Journal of Atherosclerosis and Thrombosis",
title = "CXCL16 Haplotypes in Patients with Human Carotid Atherosclerosis: Preliminary Results",
volume = "22",
number = "1",
pages = "10-20",
doi = "10.5551/jat.24299"
}
Živković, M., Đurić, T., Stojković, L. S., Jovanović, I. G., Končar, I., Davidović, L., Veljković, N. V., Alavantić, D.,& Stanković, A.. (2015). CXCL16 Haplotypes in Patients with Human Carotid Atherosclerosis: Preliminary Results. in Journal of Atherosclerosis and Thrombosis, 22(1), 10-20.
https://doi.org/10.5551/jat.24299
Živković M, Đurić T, Stojković LS, Jovanović IG, Končar I, Davidović L, Veljković NV, Alavantić D, Stanković A. CXCL16 Haplotypes in Patients with Human Carotid Atherosclerosis: Preliminary Results. in Journal of Atherosclerosis and Thrombosis. 2015;22(1):10-20.
doi:10.5551/jat.24299 .
Živković, Maja, Đurić, Tamara, Stojković, Ljiljana S., Jovanović, Ivan G., Končar, Igor, Davidović, Lazar, Veljković, Nevena V., Alavantić, Dragan, Stanković, Aleksandra, "CXCL16 Haplotypes in Patients with Human Carotid Atherosclerosis: Preliminary Results" in Journal of Atherosclerosis and Thrombosis, 22, no. 1 (2015):10-20,
https://doi.org/10.5551/jat.24299 . .
11
9
8

CXCL16 in Vascular Pathology Research: from Macro Effects to microRNAs

Jovanović, Ivan G.; Živković, Maja; Đurić, Tamara; Popović, Milan; Alavantić, Dragan; Stanković, Aleksandra

(2015)

TY  - JOUR
AU  - Jovanović, Ivan G.
AU  - Živković, Maja
AU  - Đurić, Tamara
AU  - Popović, Milan
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/818
AB  - Chemokines and their receptors have become significant factors in atherosclerosis research. CXCL16 is a multifunctional agent located on a separate locus to all other known chemokines and binds only to its unique receptor named CXCR6. As a scavenger receptor, adhesion molecule, and chemokine, it quickly became an interesting target in atherosclerosis research as all its functions have a role in vascular pathology. The investigation of the role of CXCL16 in atherosclerosis, although shown in in vitro studies, animal knockout models, and CXCL16 gene polymorphisms, haplotypes, and circulating levels, still shows puzzling results. Genetic and epigenetic studies have just scratched the surface of research necessary for a better assessment of the significance and perspective of this marker in plaque development and progression. In this review, we will summarize current knowledge about CXCL16 in atherosclerosis. Additionally, we will point out the importance of bioinformatics tools for the detection of potentially new CXCL16 regulatory networks through microRNA activity. This review aims to provide a better understanding of the underlying mechanisms, define more specific biomarkers, and discover new therapeutic targets.
T2  - Journal of Atherosclerosis and Thrombosis
T1  - CXCL16 in Vascular Pathology Research: from Macro Effects to microRNAs
VL  - 22
IS  - 10
SP  - 1012
EP  - 1024
DO  - 10.5551/jat.29942
ER  - 
@article{
author = "Jovanović, Ivan G. and Živković, Maja and Đurić, Tamara and Popović, Milan and Alavantić, Dragan and Stanković, Aleksandra",
year = "2015",
abstract = "Chemokines and their receptors have become significant factors in atherosclerosis research. CXCL16 is a multifunctional agent located on a separate locus to all other known chemokines and binds only to its unique receptor named CXCR6. As a scavenger receptor, adhesion molecule, and chemokine, it quickly became an interesting target in atherosclerosis research as all its functions have a role in vascular pathology. The investigation of the role of CXCL16 in atherosclerosis, although shown in in vitro studies, animal knockout models, and CXCL16 gene polymorphisms, haplotypes, and circulating levels, still shows puzzling results. Genetic and epigenetic studies have just scratched the surface of research necessary for a better assessment of the significance and perspective of this marker in plaque development and progression. In this review, we will summarize current knowledge about CXCL16 in atherosclerosis. Additionally, we will point out the importance of bioinformatics tools for the detection of potentially new CXCL16 regulatory networks through microRNA activity. This review aims to provide a better understanding of the underlying mechanisms, define more specific biomarkers, and discover new therapeutic targets.",
journal = "Journal of Atherosclerosis and Thrombosis",
title = "CXCL16 in Vascular Pathology Research: from Macro Effects to microRNAs",
volume = "22",
number = "10",
pages = "1012-1024",
doi = "10.5551/jat.29942"
}
Jovanović, I. G., Živković, M., Đurić, T., Popović, M., Alavantić, D.,& Stanković, A.. (2015). CXCL16 in Vascular Pathology Research: from Macro Effects to microRNAs. in Journal of Atherosclerosis and Thrombosis, 22(10), 1012-1024.
https://doi.org/10.5551/jat.29942
Jovanović IG, Živković M, Đurić T, Popović M, Alavantić D, Stanković A. CXCL16 in Vascular Pathology Research: from Macro Effects to microRNAs. in Journal of Atherosclerosis and Thrombosis. 2015;22(10):1012-1024.
doi:10.5551/jat.29942 .
Jovanović, Ivan G., Živković, Maja, Đurić, Tamara, Popović, Milan, Alavantić, Dragan, Stanković, Aleksandra, "CXCL16 in Vascular Pathology Research: from Macro Effects to microRNAs" in Journal of Atherosclerosis and Thrombosis, 22, no. 10 (2015):1012-1024,
https://doi.org/10.5551/jat.29942 . .
2
13
13
11

The At1 Receptor Polymorphism Rs5186 and Mir-155 Expression in Human Carotid Plaques

Živković, Maja; Kolaković, Ana; Đurić, Tamara; Alavantić, Dragan; Stanković, Aleksandra

(2015)

TY  - CONF
AU  - Živković, Maja
AU  - Kolaković, Ana
AU  - Đurić, Tamara
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7075
C3  - Atherosclerosis
T1  - The At1 Receptor Polymorphism Rs5186 and Mir-155 Expression in Human Carotid Plaques
VL  - 241
IS  - 1
SP  - E85
EP  - E85
DO  - 10.1016/j.atherosclerosis.2015.04.298
ER  - 
@conference{
author = "Živković, Maja and Kolaković, Ana and Đurić, Tamara and Alavantić, Dragan and Stanković, Aleksandra",
year = "2015",
journal = "Atherosclerosis",
title = "The At1 Receptor Polymorphism Rs5186 and Mir-155 Expression in Human Carotid Plaques",
volume = "241",
number = "1",
pages = "E85-E85",
doi = "10.1016/j.atherosclerosis.2015.04.298"
}
Živković, M., Kolaković, A., Đurić, T., Alavantić, D.,& Stanković, A.. (2015). The At1 Receptor Polymorphism Rs5186 and Mir-155 Expression in Human Carotid Plaques. in Atherosclerosis, 241(1), E85-E85.
https://doi.org/10.1016/j.atherosclerosis.2015.04.298
Živković M, Kolaković A, Đurić T, Alavantić D, Stanković A. The At1 Receptor Polymorphism Rs5186 and Mir-155 Expression in Human Carotid Plaques. in Atherosclerosis. 2015;241(1):E85-E85.
doi:10.1016/j.atherosclerosis.2015.04.298 .
Živković, Maja, Kolaković, Ana, Đurić, Tamara, Alavantić, Dragan, Stanković, Aleksandra, "The At1 Receptor Polymorphism Rs5186 and Mir-155 Expression in Human Carotid Plaques" in Atherosclerosis, 241, no. 1 (2015):E85-E85,
https://doi.org/10.1016/j.atherosclerosis.2015.04.298 . .