TY  - JOUR
AU  - Dojčilović, Radovan
AU  - Pajović, Jelena D.
AU  - Božanić, Dušan K.
AU  - Jović, Nataša G.
AU  - Pavlović, Vera P.
AU  - Pavlović, Vladimir B.
AU  - Lenhardt Acković, Lea
AU  - Zeković, Ivana Lj.
AU  - Dramićanin, Miroslav
AU  - Kaščakova, Slavka
AU  - Refregiers, Matthieu
AU  - Rašić, Goran
AU  - Vlahović, Branislav
AU  - Đoković, Vladimir
PY  - 2018
UR  - http://stacks.iop.org/2053-1583/5/i=4/a=045019?key=crossref.f455f07bdfb3469c8077963ff0f38f95
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7909
AB  - The interaction of partially reduced graphene oxide (prGO) and Huh7.5.1 liver cancer cells was investigated by means of DUV fluorescence bioimaging. The prGO sample was obtained by the reduction (to a certain extent) of the initially prepared graphene oxide (GO) nanosheets with hydrazine. The fluorescence of the GO nanosheets increases with time of the reduction due to a change in ratio of the sp2 and sp3 carbon sites and the prGO sample was extracted from the dispersion after 6 min, when the intensity of the fluorescence reached its maximum. The reduction process was left to proceed further to saturation until highly reduced graphene oxide (denoted here as rGO) was obtained. GO, prGO and rGO samples were investigated by structural (scanning electron microscopy (SEM), scanning transmission electron microscopy coupled with energy dispersive spectrometry (STEM-EDS)) and spectroscopic (UV-vis, photoluminescence (PL), Raman) methods. After that, Huh7.5.1 cells were incubated with GO, prGO and rGO nanosheets and used in bioimaging studies, which were performed on DISCO beamline of synchrotron SOLEIL. It was found that the prGO significantly enhanced the fluorescence of the cells and increased the intensity of the signal by ~2.5 times. Time-lapse fluorescence microscopy experiments showed that fluorescence dynamics strongly depends on the type of nanosheets used. The obtained prGO nanostructure can be easily conjugated with aromatic ring containing drugs, which opens a possibility for its applications in fluorescence microscopy monitored drug delivery. © 2018 IOP Publishing Ltd.
T2  - 2D Materials
T1  - DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells
VL  - 5
IS  - 4
SP  - 045019
DO  - 10.1088/2053-1583/aad72b
ER  - 

@article{
author = "Dojčilović, Radovan and Pajović, Jelena D. and Božanić, Dušan K. and Jović, Nataša G. and Pavlović, Vera P. and Pavlović, Vladimir B. and Lenhardt Acković, Lea and Zeković, Ivana Lj. and Dramićanin, Miroslav and Kaščakova, Slavka and Refregiers, Matthieu and Rašić, Goran and Vlahović, Branislav and Đoković, Vladimir",
year = "2018",
abstract = "The interaction of partially reduced graphene oxide (prGO) and Huh7.5.1 liver cancer cells was investigated by means of DUV fluorescence bioimaging. The prGO sample was obtained by the reduction (to a certain extent) of the initially prepared graphene oxide (GO) nanosheets with hydrazine. The fluorescence of the GO nanosheets increases with time of the reduction due to a change in ratio of the sp2 and sp3 carbon sites and the prGO sample was extracted from the dispersion after 6 min, when the intensity of the fluorescence reached its maximum. The reduction process was left to proceed further to saturation until highly reduced graphene oxide (denoted here as rGO) was obtained. GO, prGO and rGO samples were investigated by structural (scanning electron microscopy (SEM), scanning transmission electron microscopy coupled with energy dispersive spectrometry (STEM-EDS)) and spectroscopic (UV-vis, photoluminescence (PL), Raman) methods. After that, Huh7.5.1 cells were incubated with GO, prGO and rGO nanosheets and used in bioimaging studies, which were performed on DISCO beamline of synchrotron SOLEIL. It was found that the prGO significantly enhanced the fluorescence of the cells and increased the intensity of the signal by ~2.5 times. Time-lapse fluorescence microscopy experiments showed that fluorescence dynamics strongly depends on the type of nanosheets used. The obtained prGO nanostructure can be easily conjugated with aromatic ring containing drugs, which opens a possibility for its applications in fluorescence microscopy monitored drug delivery. © 2018 IOP Publishing Ltd.",
journal = "2D Materials",
title = "DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells",
volume = "5",
number = "4",
pages = "045019",
doi = "10.1088/2053-1583/aad72b"
}

Dojčilović, R., Pajović, J. D., Božanić, D. K., Jović, N. G., Pavlović, V. P., Pavlović, V. B., Lenhardt Acković, L., Zeković, I. Lj., Dramićanin, M., Kaščakova, S., Refregiers, M., Rašić, G., Vlahović, B.,& Đoković, V.. (2018). DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells. in 2D Materials, 5(4), 045019.
https://doi.org/10.1088/2053-1583/aad72b

Dojčilović R, Pajović JD, Božanić DK, Jović NG, Pavlović VP, Pavlović VB, Lenhardt Acković L, Zeković IL, Dramićanin M, Kaščakova S, Refregiers M, Rašić G, Vlahović B, Đoković V. DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells. in 2D Materials. 2018;5(4):045019.
doi:10.1088/2053-1583/aad72b .

Dojčilović, Radovan, Pajović, Jelena D., Božanić, Dušan K., Jović, Nataša G., Pavlović, Vera P., Pavlović, Vladimir B., Lenhardt Acković, Lea, Zeković, Ivana Lj., Dramićanin, Miroslav, Kaščakova, Slavka, Refregiers, Matthieu, Rašić, Goran, Vlahović, Branislav, Đoković, Vladimir, "DUV fluorescence bioimaging study of the interaction of partially reduced graphene oxide and liver cancer cells" in 2D Materials, 5, no. 4 (2018):045019,
https://doi.org/10.1088/2053-1583/aad72b . .