Nikolić, Nadežda S.

Link to this page

Authority KeyName Variants
4d93a58f-abfb-4aca-b702-1076458799f2
  • Nikolić, Nadežda S. (23)

Author's Bibliography

Synthesis and biological evaluation of Tc-99m tricarbonyl complex of O,O -diethylethylenediamine-N,N -di-3-propanoate as potential tumour diagnostic agent

Lakić, Mladen; Sabo, Ljubica; Ristic, Slavica; Savic, Aleksandar; Petricevic, Sasa; Nikolić, Nadežda S.; Vukadinović, Aleksandar; Janković, Drina; Sabo, Tibor J.; Vranješ-Đurić, Sanja

(2016)

TY  - JOUR
AU  - Lakić, Mladen
AU  - Sabo, Ljubica
AU  - Ristic, Slavica
AU  - Savic, Aleksandar
AU  - Petricevic, Sasa
AU  - Nikolić, Nadežda S.
AU  - Vukadinović, Aleksandar
AU  - Janković, Drina
AU  - Sabo, Tibor J.
AU  - Vranješ-Đurić, Sanja
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/273
AB  - The extensive development of radiopharmaceuticals towards early tumour detection and treatment has increased the demand for new ligands with higher tumour selectivity. Research has been done on the potential of the novel O,O-diethylethylenediamine-N,N-di-3-propanoate (L) ligand as a radionuclide vehicle for tumour targeting. Under alkaline conditions, L hydrolyses and produces half ester ligand (L) and diacid ligand (L), with characteristic donor atom array N, N, O. Ligand L was successfully labelled with Tc-99m at pH=9 by coordination with the octahedral fac-[Tc-99m(CO)(3)(H2O)(3)](+) intermediate, forming the main radioproduct fac-[(TcL)-Tc-99m(CO)(3)] (Tc1). The Tc-99m complex showed a low lipophilic character (log P = 0.48) and low binding affinity to human serum albumin (2.51 +/- 0.48%). In vitro stability studies in saline and human plasma, as well as challenge studies with cysteine and histidine, revealed high stability of the complex during 24 h. Biodistribution studies of Tc1 in female C57BL/6 mice bearing B16/F1 melanoma metastases showed significant tumour uptake: 9.81 +/- 1.19% ID g(-1) in the liver, 5.87 +/- 0.54% ID g(-1) in the lungs and 3.17 +/- 0.33% ID g(-1) in the ovary at 30 min post-injection. Favourable physicochemical properties, satisfactory in vitro/in vivo stability and biodistribution profile in the experimental metastatic melanoma model indicate the possible application of the radiolabelled ligand in tumour diagnosis. Copyright (C) 2015 John Wiley and Sons, Ltd.
T2  - Applied Organometallic Chemistry
T1  - Synthesis and biological evaluation of Tc-99m tricarbonyl complex of O,O -diethylethylenediamine-N,N -di-3-propanoate as potential tumour diagnostic agent
VL  - 30
IS  - 2
SP  - 81
EP  - 88
DO  - 10.1002/aoc.3401
ER  - 
@article{
author = "Lakić, Mladen and Sabo, Ljubica and Ristic, Slavica and Savic, Aleksandar and Petricevic, Sasa and Nikolić, Nadežda S. and Vukadinović, Aleksandar and Janković, Drina and Sabo, Tibor J. and Vranješ-Đurić, Sanja",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/273",
abstract = "The extensive development of radiopharmaceuticals towards early tumour detection and treatment has increased the demand for new ligands with higher tumour selectivity. Research has been done on the potential of the novel O,O-diethylethylenediamine-N,N-di-3-propanoate (L) ligand as a radionuclide vehicle for tumour targeting. Under alkaline conditions, L hydrolyses and produces half ester ligand (L) and diacid ligand (L), with characteristic donor atom array N, N, O. Ligand L was successfully labelled with Tc-99m at pH=9 by coordination with the octahedral fac-[Tc-99m(CO)(3)(H2O)(3)](+) intermediate, forming the main radioproduct fac-[(TcL)-Tc-99m(CO)(3)] (Tc1). The Tc-99m complex showed a low lipophilic character (log P = 0.48) and low binding affinity to human serum albumin (2.51 +/- 0.48%). In vitro stability studies in saline and human plasma, as well as challenge studies with cysteine and histidine, revealed high stability of the complex during 24 h. Biodistribution studies of Tc1 in female C57BL/6 mice bearing B16/F1 melanoma metastases showed significant tumour uptake: 9.81 +/- 1.19% ID g(-1) in the liver, 5.87 +/- 0.54% ID g(-1) in the lungs and 3.17 +/- 0.33% ID g(-1) in the ovary at 30 min post-injection. Favourable physicochemical properties, satisfactory in vitro/in vivo stability and biodistribution profile in the experimental metastatic melanoma model indicate the possible application of the radiolabelled ligand in tumour diagnosis. Copyright (C) 2015 John Wiley and Sons, Ltd.",
journal = "Applied Organometallic Chemistry",
title = "Synthesis and biological evaluation of Tc-99m tricarbonyl complex of O,O -diethylethylenediamine-N,N -di-3-propanoate as potential tumour diagnostic agent",
volume = "30",
number = "2",
pages = "81-88",
doi = "10.1002/aoc.3401"
}
Lakić, M., Sabo, L., Ristic, S., Savic, A., Petricevic, S., Nikolić, N. S., Vukadinović, A., Janković, D., Sabo, T. J.,& Vranješ-Đurić, S. (2016). Synthesis and biological evaluation of Tc-99m tricarbonyl complex of O,O -diethylethylenediamine-N,N -di-3-propanoate as potential tumour diagnostic agent.
Applied Organometallic Chemistry, 30(2), 81-88.
https://doi.org/10.1002/aoc.3401
Lakić M, Sabo L, Ristic S, Savic A, Petricevic S, Nikolić NS, Vukadinović A, Janković D, Sabo TJ, Vranješ-Đurić S. Synthesis and biological evaluation of Tc-99m tricarbonyl complex of O,O -diethylethylenediamine-N,N -di-3-propanoate as potential tumour diagnostic agent. Applied Organometallic Chemistry. 2016;30(2):81-88
Lakić Mladen, Sabo Ljubica, Ristic Slavica, Savic Aleksandar, Petricevic Sasa, Nikolić Nadežda S., Vukadinović Aleksandar, Janković Drina, Sabo Tibor J., Vranješ-Đurić Sanja, "Synthesis and biological evaluation of Tc-99m tricarbonyl complex of O,O -diethylethylenediamine-N,N -di-3-propanoate as potential tumour diagnostic agent" Applied Organometallic Chemistry, 30, no. 2 (2016):81-88,
https://doi.org/10.1002/aoc.3401 .
5
5
6

Preparation and in vivo evaluation of multifunctional Y-90-labeled magnetic nanoparticles designed for cancer therapy

Radović, Magdalena; Calatayud, Maria Pilar; Fabian Goya, Gerardo; Ricardo Ibarra, Manuel; Antić, Bratislav; Spasojević, Vojislav; Nikolić, Nadežda S.; Janković, Drina; Mirković, Marija D.; Vranješ-Đurić, Sanja

(2015)

TY  - JOUR
AU  - Radović, Magdalena
AU  - Calatayud, Maria Pilar
AU  - Fabian Goya, Gerardo
AU  - Ricardo Ibarra, Manuel
AU  - Antić, Bratislav
AU  - Spasojević, Vojislav
AU  - Nikolić, Nadežda S.
AU  - Janković, Drina
AU  - Mirković, Marija D.
AU  - Vranješ-Đurić, Sanja
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/234
AB  - Two different types of magnetic nanoparticles (MNPs) were synthesized in order to compare their efficiency as radioactive vectors, Fe3O4-Naked (80 +/- 5 nm) and polyethylene glycol 600 diacid functionalized Fe3O4 (Fe3O4-PEG600) MNPs (46 +/- 0.6 nm). They were characterized based on the external morphology, size distribution, and colloidal and magnetic properties. The obtained specific power absorption value for Fe3O4-PEG600 MNPs was 200 W/g, indicated their potential in hyperthermia based cancer treatments. The labeling yield, in vitro stability and in vivo biodistribution profile of Y-90-MNPs were compared. Both types of MNPs were Y-90-labeled in reproducible high yield ( GT 97%). The stability of the obtained radioactive nanoparticles was evaluated in saline and human serum media in order to optimize the formulations for in vivo use. The biodistribution in Wistar rats showed different pharmacokinetic behaviors of nanoparticles: a large fraction of both injected MNPs ended in the liver (14.58%ID/g for Y-90-Fe3O4-Naked MNPs and 19.61%ID/g for Y-90-Fe3O4-PEG600 MNPs) whereas minor fractions attained in other organs. The main difference between the two types of MNPs was the higher accumulation of Y-90-Fe3O4-Naked MNPs in the lungs (12.14%ID/g vs. 2.00%ID/g for Y-90-Fe3O4-PEG600 MNPs) due to their in vivo agglomeration. The studied radiolabeled magnetic complexes such as Y-90-Fe3O4-PEG600 MNPs constitute a great promise for multiple diagnostic-therapeutic uses combining, for example, MRI-magnetic hyperthermia and regional radiotherapy. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 126-134, 2015.
T2  - Journal of Biomedical Materials Research. Part A
T1  - Preparation and in vivo evaluation of multifunctional Y-90-labeled magnetic nanoparticles designed for cancer therapy
VL  - 103
IS  - 1
SP  - 126
EP  - 134
DO  - 10.1002/jbm.a.35160
ER  - 
@article{
author = "Radović, Magdalena and Calatayud, Maria Pilar and Fabian Goya, Gerardo and Ricardo Ibarra, Manuel and Antić, Bratislav and Spasojević, Vojislav and Nikolić, Nadežda S. and Janković, Drina and Mirković, Marija D. and Vranješ-Đurić, Sanja",
year = "2015",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/234",
abstract = "Two different types of magnetic nanoparticles (MNPs) were synthesized in order to compare their efficiency as radioactive vectors, Fe3O4-Naked (80 +/- 5 nm) and polyethylene glycol 600 diacid functionalized Fe3O4 (Fe3O4-PEG600) MNPs (46 +/- 0.6 nm). They were characterized based on the external morphology, size distribution, and colloidal and magnetic properties. The obtained specific power absorption value for Fe3O4-PEG600 MNPs was 200 W/g, indicated their potential in hyperthermia based cancer treatments. The labeling yield, in vitro stability and in vivo biodistribution profile of Y-90-MNPs were compared. Both types of MNPs were Y-90-labeled in reproducible high yield ( GT 97%). The stability of the obtained radioactive nanoparticles was evaluated in saline and human serum media in order to optimize the formulations for in vivo use. The biodistribution in Wistar rats showed different pharmacokinetic behaviors of nanoparticles: a large fraction of both injected MNPs ended in the liver (14.58%ID/g for Y-90-Fe3O4-Naked MNPs and 19.61%ID/g for Y-90-Fe3O4-PEG600 MNPs) whereas minor fractions attained in other organs. The main difference between the two types of MNPs was the higher accumulation of Y-90-Fe3O4-Naked MNPs in the lungs (12.14%ID/g vs. 2.00%ID/g for Y-90-Fe3O4-PEG600 MNPs) due to their in vivo agglomeration. The studied radiolabeled magnetic complexes such as Y-90-Fe3O4-PEG600 MNPs constitute a great promise for multiple diagnostic-therapeutic uses combining, for example, MRI-magnetic hyperthermia and regional radiotherapy. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 126-134, 2015.",
journal = "Journal of Biomedical Materials Research. Part A",
title = "Preparation and in vivo evaluation of multifunctional Y-90-labeled magnetic nanoparticles designed for cancer therapy",
volume = "103",
number = "1",
pages = "126-134",
doi = "10.1002/jbm.a.35160"
}
Radović, M., Calatayud, M. P., Fabian Goya, G., Ricardo Ibarra, M., Antić, B., Spasojević, V., Nikolić, N. S., Janković, D., Mirković, M. D.,& Vranješ-Đurić, S. (2015). Preparation and in vivo evaluation of multifunctional Y-90-labeled magnetic nanoparticles designed for cancer therapy.
Journal of Biomedical Materials Research. Part A, 103(1), 126-134.
https://doi.org/10.1002/jbm.a.35160
Radović M, Calatayud MP, Fabian Goya G, Ricardo Ibarra M, Antić B, Spasojević V, Nikolić NS, Janković D, Mirković MD, Vranješ-Đurić S. Preparation and in vivo evaluation of multifunctional Y-90-labeled magnetic nanoparticles designed for cancer therapy. Journal of Biomedical Materials Research. Part A. 2015;103(1):126-134
Radović Magdalena, Calatayud Maria Pilar, Fabian Goya Gerardo, Ricardo Ibarra Manuel, Antić Bratislav, Spasojević Vojislav, Nikolić Nadežda S., Janković Drina, Mirković Marija D., Vranješ-Đurić Sanja, "Preparation and in vivo evaluation of multifunctional Y-90-labeled magnetic nanoparticles designed for cancer therapy" Journal of Biomedical Materials Research. Part A, 103, no. 1 (2015):126-134,
https://doi.org/10.1002/jbm.a.35160 .
44
31
37

Synthesis, characterization and crystal structure of Cu(II) complex with a diimine-dioxime ligand, [Cu-2(LH)(2)](ClO4)(2). Influence of the weak Cu center dot center dot center dot O(perchlorate) interaction on the structure of the Cu2N2O2 metallocycle

Mirković, Marija D.; Nikolić, Nadežda S.; Mijin, Dušan Ž.; Ivic, Milka Avramov; Kapor, Agneš; Tomić, Zoran D.

(2014)

TY  - JOUR
AU  - Mirković, Marija D.
AU  - Nikolić, Nadežda S.
AU  - Mijin, Dušan Ž.
AU  - Ivic, Milka Avramov
AU  - Kapor, Agneš
AU  - Tomić, Zoran D.
PY  - 2014
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/6018
AB  - The diimine dioxime ligand, 3,3-(1,4-butanediyl-dinitrilo)bis-2-pentanone, 2,2-dioxime (LH2), containing a N-4 donor set was prepared by the Schiff base condensation of 2-hydroxyimino-3-pentanone and 1,4-diaminobutane in two ways: in a protic and in an aprotic solvent. A higher yield of the (LH2) imine was obtained when the synthesis was performed using a protic solvent (C2H5OH) instead of aprotic benzene (78 and 30 %, respectively). The Cu(II) metal complex of diimine dioxime was synthesized in CH3OH from the perchlorate salt of LH2 in a 1:1 mole ratio. The isolated complex was characterized by the elemental analysis, IR spectroscopy and cyclic voltammetry. The structure of [Cu-2(LH)(2)](ClO4)(2) was determined by single-crystal X-ray diffraction analysis. Comparison with structurally related diimine dioxime Cu(II) complexes revealed the influence of a weak Cu center dot center dot center dot O(perchlorate) interaction on the geometry of the metallocycle.
T2  - Journal of the Serbian Chemical Society
T1  - Synthesis, characterization and crystal structure of Cu(II) complex with a diimine-dioxime ligand, [Cu-2(LH)(2)](ClO4)(2). Influence of the weak Cu center dot center dot center dot O(perchlorate) interaction on the structure of the Cu2N2O2 metallocycle
VL  - 79
IS  - 5
SP  - 545
EP  - 556
DO  - 10.2298/JSC130910120M
ER  - 
@article{
author = "Mirković, Marija D. and Nikolić, Nadežda S. and Mijin, Dušan Ž. and Ivic, Milka Avramov and Kapor, Agneš and Tomić, Zoran D.",
year = "2014",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/6018",
abstract = "The diimine dioxime ligand, 3,3-(1,4-butanediyl-dinitrilo)bis-2-pentanone, 2,2-dioxime (LH2), containing a N-4 donor set was prepared by the Schiff base condensation of 2-hydroxyimino-3-pentanone and 1,4-diaminobutane in two ways: in a protic and in an aprotic solvent. A higher yield of the (LH2) imine was obtained when the synthesis was performed using a protic solvent (C2H5OH) instead of aprotic benzene (78 and 30 %, respectively). The Cu(II) metal complex of diimine dioxime was synthesized in CH3OH from the perchlorate salt of LH2 in a 1:1 mole ratio. The isolated complex was characterized by the elemental analysis, IR spectroscopy and cyclic voltammetry. The structure of [Cu-2(LH)(2)](ClO4)(2) was determined by single-crystal X-ray diffraction analysis. Comparison with structurally related diimine dioxime Cu(II) complexes revealed the influence of a weak Cu center dot center dot center dot O(perchlorate) interaction on the geometry of the metallocycle.",
journal = "Journal of the Serbian Chemical Society",
title = "Synthesis, characterization and crystal structure of Cu(II) complex with a diimine-dioxime ligand, [Cu-2(LH)(2)](ClO4)(2). Influence of the weak Cu center dot center dot center dot O(perchlorate) interaction on the structure of the Cu2N2O2 metallocycle",
volume = "79",
number = "5",
pages = "545-556",
doi = "10.2298/JSC130910120M"
}
Mirković, M. D., Nikolić, N. S., Mijin, D. Ž., Ivic, M. A., Kapor, A.,& Tomić, Z. D. (2014). Synthesis, characterization and crystal structure of Cu(II) complex with a diimine-dioxime ligand, [Cu-2(LH)(2)](ClO4)(2). Influence of the weak Cu center dot center dot center dot O(perchlorate) interaction on the structure of the Cu2N2O2 metallocycle.
Journal of the Serbian Chemical Society, 79(5), 545-556.
https://doi.org/10.2298/JSC130910120M
Mirković MD, Nikolić NS, Mijin DŽ, Ivic MA, Kapor A, Tomić ZD. Synthesis, characterization and crystal structure of Cu(II) complex with a diimine-dioxime ligand, [Cu-2(LH)(2)](ClO4)(2). Influence of the weak Cu center dot center dot center dot O(perchlorate) interaction on the structure of the Cu2N2O2 metallocycle. Journal of the Serbian Chemical Society. 2014;79(5):545-556
Mirković Marija D., Nikolić Nadežda S., Mijin Dušan Ž., Ivic Milka Avramov, Kapor Agneš, Tomić Zoran D., "Synthesis, characterization and crystal structure of Cu(II) complex with a diimine-dioxime ligand, [Cu-2(LH)(2)](ClO4)(2). Influence of the weak Cu center dot center dot center dot O(perchlorate) interaction on the structure of the Cu2N2O2 metallocycle" Journal of the Serbian Chemical Society, 79, no. 5 (2014):545-556,
https://doi.org/10.2298/JSC130910120M .

Electrochemical Separation of 90-Yttrium in the Electrochemical Sr-90/Y-90 Generator and Its Use for Radiolabelling of Dota-Conjugated Somatostatin Analog [Dota(0), Tyr(3)] Octreotate

Petrović, Đorđe; Nikolić, Nadežda S.; Stanković, Dragana; Đokić, Divna Đ.

(2012)

TY  - JOUR
AU  - Petrović, Đorđe
AU  - Nikolić, Nadežda S.
AU  - Stanković, Dragana
AU  - Đokić, Divna Đ.
PY  - 2012
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5069
AB  - Radiopharmaceuticals based on Y-90 are widely used in the treatment of malignant deseases. In order to meet the requirements for their future application, a Sr-90/Y-90 generator was developed and 90Y eluted from this locally produced generator was used for the radiolabelling of the DOTA-conjugated somatostatin analog [DOTA(0),Tyr(3)] octreotate and the preparation of [Y-90-DOTA(0),Tyr(3)] octreotate (Y-90-DOTATATE) for peptide receptore radionuclide therapy. Sr-90/Y-90 generator was based on the electrochemical separation of Y-90 from Sr-90 in a two-cycle electrolysis procedure. Three electrode cells were used to perform both electrolyses. In both cycles, working electrodes were kept on constant potential. The pH of the solution was adjusted to 2.7 of the value before the electrolyses. The radionucliclic purity of the Y-90 solution was analysed by ITLC and extraction paper chromatography. The labelling of peptide (100 mu g DOTATATE) with (YCl3)-Y-90 was performed at 95 degrees C for 30 minutes. Radiochemical purity was determined by HPLC and chromatographic separation, using a solid SepPak C-18 column. Results obtained confirmed the efficiency of our electrochemical separation technique and quality control methods for Y-90. The achieved efficiency of the Sr-90/Y-90 generator above 96% of the theoretical value represents a good basis for the further development of this generator. The labelling of the DOTATATE with Y-90 exhibited a high efficiency, too: there was less than 1% of Y-90(3+) in the Y-90-DOTATATE.
T2  - Nuclear technology and radiation protection
T1  - Electrochemical Separation of 90-Yttrium in the Electrochemical Sr-90/Y-90 Generator and Its Use for Radiolabelling of Dota-Conjugated Somatostatin Analog [Dota(0), Tyr(3)] Octreotate
VL  - 27
IS  - 3
SP  - 260
EP  - 268
DO  - 10.2298/NTRP1203260P
ER  - 
@article{
author = "Petrović, Đorđe and Nikolić, Nadežda S. and Stanković, Dragana and Đokić, Divna Đ.",
year = "2012",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/5069",
abstract = "Radiopharmaceuticals based on Y-90 are widely used in the treatment of malignant deseases. In order to meet the requirements for their future application, a Sr-90/Y-90 generator was developed and 90Y eluted from this locally produced generator was used for the radiolabelling of the DOTA-conjugated somatostatin analog [DOTA(0),Tyr(3)] octreotate and the preparation of [Y-90-DOTA(0),Tyr(3)] octreotate (Y-90-DOTATATE) for peptide receptore radionuclide therapy. Sr-90/Y-90 generator was based on the electrochemical separation of Y-90 from Sr-90 in a two-cycle electrolysis procedure. Three electrode cells were used to perform both electrolyses. In both cycles, working electrodes were kept on constant potential. The pH of the solution was adjusted to 2.7 of the value before the electrolyses. The radionucliclic purity of the Y-90 solution was analysed by ITLC and extraction paper chromatography. The labelling of peptide (100 mu g DOTATATE) with (YCl3)-Y-90 was performed at 95 degrees C for 30 minutes. Radiochemical purity was determined by HPLC and chromatographic separation, using a solid SepPak C-18 column. Results obtained confirmed the efficiency of our electrochemical separation technique and quality control methods for Y-90. The achieved efficiency of the Sr-90/Y-90 generator above 96% of the theoretical value represents a good basis for the further development of this generator. The labelling of the DOTATATE with Y-90 exhibited a high efficiency, too: there was less than 1% of Y-90(3+) in the Y-90-DOTATATE.",
journal = "Nuclear technology and radiation protection",
title = "Electrochemical Separation of 90-Yttrium in the Electrochemical Sr-90/Y-90 Generator and Its Use for Radiolabelling of Dota-Conjugated Somatostatin Analog [Dota(0), Tyr(3)] Octreotate",
volume = "27",
number = "3",
pages = "260-268",
doi = "10.2298/NTRP1203260P"
}
Petrović, Đ., Nikolić, N. S., Stanković, D.,& Đokić, D. Đ. (2012). Electrochemical Separation of 90-Yttrium in the Electrochemical Sr-90/Y-90 Generator and Its Use for Radiolabelling of Dota-Conjugated Somatostatin Analog [Dota(0), Tyr(3)] Octreotate.
Nuclear technology and radiation protection, 27(3), 260-268.
https://doi.org/10.2298/NTRP1203260P
Petrović Đ, Nikolić NS, Stanković D, Đokić DĐ. Electrochemical Separation of 90-Yttrium in the Electrochemical Sr-90/Y-90 Generator and Its Use for Radiolabelling of Dota-Conjugated Somatostatin Analog [Dota(0), Tyr(3)] Octreotate. Nuclear technology and radiation protection. 2012;27(3):260-268
Petrović Đorđe, Nikolić Nadežda S., Stanković Dragana, Đokić Divna Đ., "Electrochemical Separation of 90-Yttrium in the Electrochemical Sr-90/Y-90 Generator and Its Use for Radiolabelling of Dota-Conjugated Somatostatin Analog [Dota(0), Tyr(3)] Octreotate" Nuclear technology and radiation protection, 27, no. 3 (2012):260-268,
https://doi.org/10.2298/NTRP1203260P .
1
4
4

Novel tetradentate diamine dioxime ligands: synthesis, characterization and in vivo behavior of their 99mTc-complexes

Mirković, Marija D.; Janković, Drina; Vranješ-Đurić, Sanja; Radović, Magdalena; Stanković, Dragana; Mijin, Dušan Ž.; Nikolić, Nadežda S.

(2012)

TY  - JOUR
AU  - Mirković, Marija D.
AU  - Janković, Drina
AU  - Vranješ-Đurić, Sanja
AU  - Radović, Magdalena
AU  - Stanković, Dragana
AU  - Mijin, Dušan Ž.
AU  - Nikolić, Nadežda S.
PY  - 2012
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4899
AB  - Two novel diamine dioxime ligands, 4,7-diaza-3,8-diethyldecane-2,9-dione bis oxime (3) and 4,9-diaza-3,10-diethyldodecane-2,11-dione bis oxime (5), were synthesized in order to develop new brain perfusion imaging agents, based on 99mTc(V)-complexes. The synthesis involved condensation of 2-hydroxyimino-3-pentanone with appropriate diamine in protic solvent which afforded the bis imine adducts. Subsequent reduction of imine functional groups yielded a diastereoisomeric mixture of 3 and 5. UVvisible, IR, 1H NMR, 13C NMR and elemental analysis were used to characterize the structures of the synthesized compounds. 99mTc-complexes of both diamine dioximes were prepared and radiolabeling conditions optimized to give the maximum yield. Physicochemical parameters of the labeled complexes as well as and their biodistribution in rats were investigated. Both compounds (3 and 5) formed 99mTc-complexes with a net charge of zero, determined by electrophoresis. The resultant lipophilic 99mTc-complexes of 3 and 5 were readily formed at pH similar to 9.0 within 10?min at room temperature with yields of 90% and 95%, respectively. The 99mTc-3 complex was found to be stable within 1 similar to h, while 99mTc-5 was stable for a few hours. A significant brain uptake of 99mTc-3 (2.1% injected dose) and 99mTc-5 (1.8% injected dose) complexes, 2?min after injection, is in accordance with their lipophilicity. The present study suggests that both ligands are promising candidates as new 99mTc-based brain-imaging agents. Copyright (c) 2012 John Wiley and Sons, Ltd.
T2  - Applied Organometallic Chemistry
T1  - Novel tetradentate diamine dioxime ligands: synthesis, characterization and in vivo behavior of their 99mTc-complexes
VL  - 26
IS  - 7
SP  - 347
EP  - 355
DO  - 10.1002/aoc.2870
ER  - 
@article{
author = "Mirković, Marija D. and Janković, Drina and Vranješ-Đurić, Sanja and Radović, Magdalena and Stanković, Dragana and Mijin, Dušan Ž. and Nikolić, Nadežda S.",
year = "2012",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/4899",
abstract = "Two novel diamine dioxime ligands, 4,7-diaza-3,8-diethyldecane-2,9-dione bis oxime (3) and 4,9-diaza-3,10-diethyldodecane-2,11-dione bis oxime (5), were synthesized in order to develop new brain perfusion imaging agents, based on 99mTc(V)-complexes. The synthesis involved condensation of 2-hydroxyimino-3-pentanone with appropriate diamine in protic solvent which afforded the bis imine adducts. Subsequent reduction of imine functional groups yielded a diastereoisomeric mixture of 3 and 5. UVvisible, IR, 1H NMR, 13C NMR and elemental analysis were used to characterize the structures of the synthesized compounds. 99mTc-complexes of both diamine dioximes were prepared and radiolabeling conditions optimized to give the maximum yield. Physicochemical parameters of the labeled complexes as well as and their biodistribution in rats were investigated. Both compounds (3 and 5) formed 99mTc-complexes with a net charge of zero, determined by electrophoresis. The resultant lipophilic 99mTc-complexes of 3 and 5 were readily formed at pH similar to 9.0 within 10?min at room temperature with yields of 90% and 95%, respectively. The 99mTc-3 complex was found to be stable within 1 similar to h, while 99mTc-5 was stable for a few hours. A significant brain uptake of 99mTc-3 (2.1% injected dose) and 99mTc-5 (1.8% injected dose) complexes, 2?min after injection, is in accordance with their lipophilicity. The present study suggests that both ligands are promising candidates as new 99mTc-based brain-imaging agents. Copyright (c) 2012 John Wiley and Sons, Ltd.",
journal = "Applied Organometallic Chemistry",
title = "Novel tetradentate diamine dioxime ligands: synthesis, characterization and in vivo behavior of their 99mTc-complexes",
volume = "26",
number = "7",
pages = "347-355",
doi = "10.1002/aoc.2870"
}
Mirković, M. D., Janković, D., Vranješ-Đurić, S., Radović, M., Stanković, D., Mijin, D. Ž.,& Nikolić, N. S. (2012). Novel tetradentate diamine dioxime ligands: synthesis, characterization and in vivo behavior of their 99mTc-complexes.
Applied Organometallic Chemistry, 26(7), 347-355.
https://doi.org/10.1002/aoc.2870
Mirković MD, Janković D, Vranješ-Đurić S, Radović M, Stanković D, Mijin DŽ, Nikolić NS. Novel tetradentate diamine dioxime ligands: synthesis, characterization and in vivo behavior of their 99mTc-complexes. Applied Organometallic Chemistry. 2012;26(7):347-355
Mirković Marija D., Janković Drina, Vranješ-Đurić Sanja, Radović Magdalena, Stanković Dragana, Mijin Dušan Ž., Nikolić Nadežda S., "Novel tetradentate diamine dioxime ligands: synthesis, characterization and in vivo behavior of their 99mTc-complexes" Applied Organometallic Chemistry, 26, no. 7 (2012):347-355,
https://doi.org/10.1002/aoc.2870 .
4
4
5

90Y-labeled tin fluoride colloid as a promising therapeutic agent: Preparation, characterization, and biological study in rats

Janković, Drina; Vranješ-Đurić, Sanja; Đokić, Divna Đ.; Marković, Mirjana; Ajdinovic, Boris; Jaukovic, Ljiljana; Nikolić, Nadežda S.

(2012)

TY  - JOUR
AU  - Janković, Drina
AU  - Vranješ-Đurić, Sanja
AU  - Đokić, Divna Đ.
AU  - Marković, Mirjana
AU  - Ajdinovic, Boris
AU  - Jaukovic, Ljiljana
AU  - Nikolić, Nadežda S.
PY  - 2012
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4800
AB  - In this study, tin fluoride colloid (SnF-c) was prepared, labeled with yttrium-90 (90Y), and characterized with respect to its physicochemical properties and biological behavior in an animal model. Particle size of SnF-c, at constant concentration of SnF2, was dependent on pH, concentration of sodium fluoride (NaF), temperature, and time. The particle size of SnF-c decreased with an increase in NaF concentration and a decrease in reaction mixture pH. Radiolabeling yield of 90YSnF-c at higher temperature increased and it was greater than 98% for the preparation at 95 degrees C. The 90YSnF-c demonstrated high in vitro stability both in human serum and human synovial fluid at 37 degrees C up to 7 days. In vivo distribution studies in healthy male Wistar rats of 90YSnF-c (particles LT 1 mu m), following intravenous administration, revealed that the localization takes place preferably in the liver. The 90YSnF-c (particles GT 1 mu m) was well retained in the synovial space for 96 h after intra-articular injection, whereas leakage of 90Y from the joint was 1.96% over this period. Because of high labeling yield and stability, 90YSnF-c might be a promising agent for radiosynovectomy or therapy of liver malignancies. (C) 2012 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 101:21942203, 2012
T2  - Journal of Pharmaceutical Sciences
T1  - 90Y-labeled tin fluoride colloid as a promising therapeutic agent: Preparation, characterization, and biological study in rats
VL  - 101
IS  - 6
SP  - 2194
EP  - 2203
DO  - 10.1002/jps.23114
ER  - 
@article{
author = "Janković, Drina and Vranješ-Đurić, Sanja and Đokić, Divna Đ. and Marković, Mirjana and Ajdinovic, Boris and Jaukovic, Ljiljana and Nikolić, Nadežda S.",
year = "2012",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/4800",
abstract = "In this study, tin fluoride colloid (SnF-c) was prepared, labeled with yttrium-90 (90Y), and characterized with respect to its physicochemical properties and biological behavior in an animal model. Particle size of SnF-c, at constant concentration of SnF2, was dependent on pH, concentration of sodium fluoride (NaF), temperature, and time. The particle size of SnF-c decreased with an increase in NaF concentration and a decrease in reaction mixture pH. Radiolabeling yield of 90YSnF-c at higher temperature increased and it was greater than 98% for the preparation at 95 degrees C. The 90YSnF-c demonstrated high in vitro stability both in human serum and human synovial fluid at 37 degrees C up to 7 days. In vivo distribution studies in healthy male Wistar rats of 90YSnF-c (particles LT 1 mu m), following intravenous administration, revealed that the localization takes place preferably in the liver. The 90YSnF-c (particles GT 1 mu m) was well retained in the synovial space for 96 h after intra-articular injection, whereas leakage of 90Y from the joint was 1.96% over this period. Because of high labeling yield and stability, 90YSnF-c might be a promising agent for radiosynovectomy or therapy of liver malignancies. (C) 2012 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 101:21942203, 2012",
journal = "Journal of Pharmaceutical Sciences",
title = "90Y-labeled tin fluoride colloid as a promising therapeutic agent: Preparation, characterization, and biological study in rats",
volume = "101",
number = "6",
pages = "2194-2203",
doi = "10.1002/jps.23114"
}
Janković, D., Vranješ-Đurić, S., Đokić, D. Đ., Marković, M., Ajdinovic, B., Jaukovic, L.,& Nikolić, N. S. (2012). 90Y-labeled tin fluoride colloid as a promising therapeutic agent: Preparation, characterization, and biological study in rats.
Journal of Pharmaceutical Sciences, 101(6), 2194-2203.
https://doi.org/10.1002/jps.23114
Janković D, Vranješ-Đurić S, Đokić DĐ, Marković M, Ajdinovic B, Jaukovic L, Nikolić NS. 90Y-labeled tin fluoride colloid as a promising therapeutic agent: Preparation, characterization, and biological study in rats. Journal of Pharmaceutical Sciences. 2012;101(6):2194-2203
Janković Drina, Vranješ-Đurić Sanja, Đokić Divna Đ., Marković Mirjana, Ajdinovic Boris, Jaukovic Ljiljana, Nikolić Nadežda S., "90Y-labeled tin fluoride colloid as a promising therapeutic agent: Preparation, characterization, and biological study in rats" Journal of Pharmaceutical Sciences, 101, no. 6 (2012):2194-2203,
https://doi.org/10.1002/jps.23114 .
8
8
9

Development and evaluation of Y-90-labeled albumin microspheres loaded with magnetite nanoparticles for possible applications in cancer therapy

Radović, Magdalena; Vranješ-Đurić, Sanja; Nikolić, Nadežda S.; Janković, Drina; Goya, Gerardo F.; Torres, Teobaldo E.; Calatayud, Maria Pilar; Bruvera, Ignacio J.; Ibarra, M. Ricardo; Spasojević, Vojislav; Jančar, Boštjan; Antić, Bratislav

(2012)

TY  - JOUR
AU  - Radović, Magdalena
AU  - Vranješ-Đurić, Sanja
AU  - Nikolić, Nadežda S.
AU  - Janković, Drina
AU  - Goya, Gerardo F.
AU  - Torres, Teobaldo E.
AU  - Calatayud, Maria Pilar
AU  - Bruvera, Ignacio J.
AU  - Ibarra, M. Ricardo
AU  - Spasojević, Vojislav
AU  - Jančar, Boštjan
AU  - Antić, Bratislav
PY  - 2012
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/5175
AB  - Radio labeled albumin microspheres with encapsulated citric acid-coated magnetite nanoparticles were developed as a targeting approach to localize both radioactivity and magnetic energy at the tumor site. We present in vitro and in vivo studies of yttrium-90 (Y-90)-labeled human scrum albumin magnetic microspheres (HSAMMS) as a multifunctional agent for possible applications in a bimodal radionuclide-hyperthermia cancer therapy. The HSAMMS were produced using a modified emulsification-heat stabilization technique and contained 11 nm magnetite nanoparticles coated with citric acid, distributed as inhomogeneous clusters within the albumin microspheres. The size, size distribution and the morphology of magnetite nanoparticles and HSAMMS were determined by FESEM, HRTEM and SEM/FIB dual beam. The average particle size of the complete HSAMMS was 20 mu m, and they exhibited superparamagnetic behavior at room temperature. The in vitro experiments (in saline and human serum) revealed the high stability of the labeled HSAMMS in saline and human serum after 72 h. Following the intravenous administration of the Y-90-HSAMMS in rats, 88.81% of the activity localizes in the lungs after 1 h, with 82.67% remaining after 72 h. These data on Y-90-HSAMMS provide good evidence for their potential use in bimodal radionuclide-hyperthermia cancer therapy.
T2  - Journal of Materials Chemistry
T1  - Development and evaluation of Y-90-labeled albumin microspheres loaded with magnetite nanoparticles for possible applications in cancer therapy
VL  - 22
IS  - 45
SP  - 24017
EP  - 24025
DO  - 10.1039/c2jm35593k
ER  - 
@article{
author = "Radović, Magdalena and Vranješ-Đurić, Sanja and Nikolić, Nadežda S. and Janković, Drina and Goya, Gerardo F. and Torres, Teobaldo E. and Calatayud, Maria Pilar and Bruvera, Ignacio J. and Ibarra, M. Ricardo and Spasojević, Vojislav and Jančar, Boštjan and Antić, Bratislav",
year = "2012",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/5175",
abstract = "Radio labeled albumin microspheres with encapsulated citric acid-coated magnetite nanoparticles were developed as a targeting approach to localize both radioactivity and magnetic energy at the tumor site. We present in vitro and in vivo studies of yttrium-90 (Y-90)-labeled human scrum albumin magnetic microspheres (HSAMMS) as a multifunctional agent for possible applications in a bimodal radionuclide-hyperthermia cancer therapy. The HSAMMS were produced using a modified emulsification-heat stabilization technique and contained 11 nm magnetite nanoparticles coated with citric acid, distributed as inhomogeneous clusters within the albumin microspheres. The size, size distribution and the morphology of magnetite nanoparticles and HSAMMS were determined by FESEM, HRTEM and SEM/FIB dual beam. The average particle size of the complete HSAMMS was 20 mu m, and they exhibited superparamagnetic behavior at room temperature. The in vitro experiments (in saline and human serum) revealed the high stability of the labeled HSAMMS in saline and human serum after 72 h. Following the intravenous administration of the Y-90-HSAMMS in rats, 88.81% of the activity localizes in the lungs after 1 h, with 82.67% remaining after 72 h. These data on Y-90-HSAMMS provide good evidence for their potential use in bimodal radionuclide-hyperthermia cancer therapy.",
journal = "Journal of Materials Chemistry",
title = "Development and evaluation of Y-90-labeled albumin microspheres loaded with magnetite nanoparticles for possible applications in cancer therapy",
volume = "22",
number = "45",
pages = "24017-24025",
doi = "10.1039/c2jm35593k"
}
Radović, M., Vranješ-Đurić, S., Nikolić, N. S., Janković, D., Goya, G. F., Torres, T. E., Calatayud, M. P., Bruvera, I. J., Ibarra, M. R., Spasojević, V., Jančar, B.,& Antić, B. (2012). Development and evaluation of Y-90-labeled albumin microspheres loaded with magnetite nanoparticles for possible applications in cancer therapy.
Journal of Materials Chemistry, 22(45), 24017-24025.
https://doi.org/10.1039/c2jm35593k
Radović M, Vranješ-Đurić S, Nikolić NS, Janković D, Goya GF, Torres TE, Calatayud MP, Bruvera IJ, Ibarra MR, Spasojević V, Jančar B, Antić B. Development and evaluation of Y-90-labeled albumin microspheres loaded with magnetite nanoparticles for possible applications in cancer therapy. Journal of Materials Chemistry. 2012;22(45):24017-24025
Radović Magdalena, Vranješ-Đurić Sanja, Nikolić Nadežda S., Janković Drina, Goya Gerardo F., Torres Teobaldo E., Calatayud Maria Pilar, Bruvera Ignacio J., Ibarra M. Ricardo, Spasojević Vojislav, Jančar Boštjan, Antić Bratislav, "Development and evaluation of Y-90-labeled albumin microspheres loaded with magnetite nanoparticles for possible applications in cancer therapy" Journal of Materials Chemistry, 22, no. 45 (2012):24017-24025,
https://doi.org/10.1039/c2jm35593k .
1
26
17
24

Benzimidazole derivatives as NSO ligands for the fac-[M(CO)(3)](+) (M = Re, Tc-99m)

Tsotakos, T.; Tsoukalas, C.; Patsis, G.; Panagiotopoulou, A.; Nikolić, Nadežda S.; Janković, Drina; Đokić, Divna Đ.; Raptopoulou, C. P.; Terzis, A.; Papagiannopoulou, D.; Pelecanou, M.; Papadopoulos, M.; Pirmettis, I.

(2011)

TY  - JOUR
AU  - Tsotakos, T.
AU  - Tsoukalas, C.
AU  - Patsis, G.
AU  - Panagiotopoulou, A.
AU  - Nikolić, Nadežda S.
AU  - Janković, Drina
AU  - Đokić, Divna Đ.
AU  - Raptopoulou, C. P.
AU  - Terzis, A.
AU  - Papagiannopoulou, D.
AU  - Pelecanou, M.
AU  - Papadopoulos, M.
AU  - Pirmettis, I.
PY  - 2011
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4546
AB  - Two rhenium(I) tricarbonyl complexes with the tridentate monoanionic NSO ligands, 4-(benzimidazol-2-yl)-3-thiabutanoic acid (complex 3) and [1-(11-carboxyundecanyl)-4-(benzimidazol-2-yl)]-3-thiabutanoic acid (complex 4) were synthesized and characterized by spectroscopic methods and elemental analysis. X-ray crystallographic analysis of complex 3 revealed a distorted octahedral geometry around rhenium defined by the three facially bound CO groups and the NSO donor atom set of the tridentate ligand. The analogous technetium-99m complexes (complexes 5 and 6) were also prepared quantitatively by reaction of the NSO ligands with the fac-[Tc-99m(H2O)(3)(CO)(3)](+) synthon and their identity was established by chromatographic comparison to their rhenium congeners. Biodistribution in mice of complex 6 bearing the fatty acid chain showed significant heart uptake (6.26 +/- 0.79% ID/g p.i.) at 1 min accompanied, however, with a heart: blood ratio below 1. (C) 2011 Elsevier B. V. All rights reserved.
T2  - Inorganica Chimica Acta
T1  - Benzimidazole derivatives as NSO ligands for the fac-[M(CO)(3)](+) (M = Re, Tc-99m)
VL  - 377
IS  - 1
SP  - 62
EP  - 68
DO  - 10.1016/j.ica.2011.07.035
ER  - 
@article{
author = "Tsotakos, T. and Tsoukalas, C. and Patsis, G. and Panagiotopoulou, A. and Nikolić, Nadežda S. and Janković, Drina and Đokić, Divna Đ. and Raptopoulou, C. P. and Terzis, A. and Papagiannopoulou, D. and Pelecanou, M. and Papadopoulos, M. and Pirmettis, I.",
year = "2011",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/4546",
abstract = "Two rhenium(I) tricarbonyl complexes with the tridentate monoanionic NSO ligands, 4-(benzimidazol-2-yl)-3-thiabutanoic acid (complex 3) and [1-(11-carboxyundecanyl)-4-(benzimidazol-2-yl)]-3-thiabutanoic acid (complex 4) were synthesized and characterized by spectroscopic methods and elemental analysis. X-ray crystallographic analysis of complex 3 revealed a distorted octahedral geometry around rhenium defined by the three facially bound CO groups and the NSO donor atom set of the tridentate ligand. The analogous technetium-99m complexes (complexes 5 and 6) were also prepared quantitatively by reaction of the NSO ligands with the fac-[Tc-99m(H2O)(3)(CO)(3)](+) synthon and their identity was established by chromatographic comparison to their rhenium congeners. Biodistribution in mice of complex 6 bearing the fatty acid chain showed significant heart uptake (6.26 +/- 0.79% ID/g p.i.) at 1 min accompanied, however, with a heart: blood ratio below 1. (C) 2011 Elsevier B. V. All rights reserved.",
journal = "Inorganica Chimica Acta",
title = "Benzimidazole derivatives as NSO ligands for the fac-[M(CO)(3)](+) (M = Re, Tc-99m)",
volume = "377",
number = "1",
pages = "62-68",
doi = "10.1016/j.ica.2011.07.035"
}
Tsotakos, T., Tsoukalas, C., Patsis, G., Panagiotopoulou, A., Nikolić, N. S., Janković, D., Đokić, D. Đ., Raptopoulou, C. P., Terzis, A., Papagiannopoulou, D., Pelecanou, M., Papadopoulos, M.,& Pirmettis, I. (2011). Benzimidazole derivatives as NSO ligands for the fac-[M(CO)(3)](+) (M = Re, Tc-99m).
Inorganica Chimica Acta, 377(1), 62-68.
https://doi.org/10.1016/j.ica.2011.07.035
Tsotakos T, Tsoukalas C, Patsis G, Panagiotopoulou A, Nikolić NS, Janković D, Đokić DĐ, Raptopoulou CP, Terzis A, Papagiannopoulou D, Pelecanou M, Papadopoulos M, Pirmettis I. Benzimidazole derivatives as NSO ligands for the fac-[M(CO)(3)](+) (M = Re, Tc-99m). Inorganica Chimica Acta. 2011;377(1):62-68
Tsotakos T., Tsoukalas C., Patsis G., Panagiotopoulou A., Nikolić Nadežda S., Janković Drina, Đokić Divna Đ., Raptopoulou C. P., Terzis A., Papagiannopoulou D., Pelecanou M., Papadopoulos M., Pirmettis I., "Benzimidazole derivatives as NSO ligands for the fac-[M(CO)(3)](+) (M = Re, Tc-99m)" Inorganica Chimica Acta, 377, no. 1 (2011):62-68,
https://doi.org/10.1016/j.ica.2011.07.035 .
5
6
5

Analysis of the yttrium-90-labelled human serum albumin microspheres

Đokić, Divna Đ.; Janković, Drina; Vranješ-Đurić, Sanja; Nikolić, Nadežda S.

(2010)

TY  - CONF
AU  - Đokić, Divna Đ.
AU  - Janković, Drina
AU  - Vranješ-Đurić, Sanja
AU  - Nikolić, Nadežda S.
PY  - 2010
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4083
C3  - Nuclear Medicine and Biology
T1  - Analysis of the yttrium-90-labelled human serum albumin microspheres
VL  - 37
IS  - 6
SP  - 696
EP  - 696
DO  - 10.1016/j.nucmedbio.2010.04.184
ER  - 
@conference{
author = "Đokić, Divna Đ. and Janković, Drina and Vranješ-Đurić, Sanja and Nikolić, Nadežda S.",
year = "2010",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/4083",
journal = "Nuclear Medicine and Biology",
title = "Analysis of the yttrium-90-labelled human serum albumin microspheres",
volume = "37",
number = "6",
pages = "696-696",
doi = "10.1016/j.nucmedbio.2010.04.184"
}
Đokić, D. Đ., Janković, D., Vranješ-Đurić, S.,& Nikolić, N. S. (2010). Analysis of the yttrium-90-labelled human serum albumin microspheres.
Nuclear Medicine and Biology, 37(6), 696-696.
https://doi.org/10.1016/j.nucmedbio.2010.04.184
Đokić DĐ, Janković D, Vranješ-Đurić S, Nikolić NS. Analysis of the yttrium-90-labelled human serum albumin microspheres. Nuclear Medicine and Biology. 2010;37(6):696-696
Đokić Divna Đ., Janković Drina, Vranješ-Đurić Sanja, Nikolić Nadežda S., "Analysis of the yttrium-90-labelled human serum albumin microspheres" Nuclear Medicine and Biology, 37, no. 6 (2010):696-696,
https://doi.org/10.1016/j.nucmedbio.2010.04.184 .

Modified 90Y-hydroxyapatite microparticles, possible agent for lung cancer therapy

Nikolić, Nadežda S.; Vranješ-Đurić, Sanja; Janković, Drina

(2010)

TY  - CONF
AU  - Nikolić, Nadežda S.
AU  - Vranješ-Đurić, Sanja
AU  - Janković, Drina
PY  - 2010
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4082
C3  - Nuclear Medicine and Biology
T1  - Modified 90Y-hydroxyapatite microparticles, possible agent for lung cancer therapy
VL  - 37
IS  - 6
SP  - 696
EP  - 696
DO  - 10.1016/j.nucmedbio.2010.04.167
ER  - 
@conference{
author = "Nikolić, Nadežda S. and Vranješ-Đurić, Sanja and Janković, Drina",
year = "2010",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/4082",
journal = "Nuclear Medicine and Biology",
title = "Modified 90Y-hydroxyapatite microparticles, possible agent for lung cancer therapy",
volume = "37",
number = "6",
pages = "696-696",
doi = "10.1016/j.nucmedbio.2010.04.167"
}
Nikolić, N. S., Vranješ-Đurić, S.,& Janković, D. (2010). Modified 90Y-hydroxyapatite microparticles, possible agent for lung cancer therapy.
Nuclear Medicine and Biology, 37(6), 696-696.
https://doi.org/10.1016/j.nucmedbio.2010.04.167
Nikolić NS, Vranješ-Đurić S, Janković D. Modified 90Y-hydroxyapatite microparticles, possible agent for lung cancer therapy. Nuclear Medicine and Biology. 2010;37(6):696-696
Nikolić Nadežda S., Vranješ-Đurić Sanja, Janković Drina, "Modified 90Y-hydroxyapatite microparticles, possible agent for lung cancer therapy" Nuclear Medicine and Biology, 37, no. 6 (2010):696-696,
https://doi.org/10.1016/j.nucmedbio.2010.04.167 .
1

Rhenium(I) and technetium-99m(I) fac-tricarbonyl complexes with 4-(imidazolin-2-yl)-3-thiabutanoic acid derivatives as tridentate ligands: Synthesis and structural characterization

Kyprianidou, P.; Tsoukalas, C.; Patsis, G.; Papagiannopoulou, D.; Nikolić, Nadežda S.; Janković, Drina; Đokić, Divna Đ.; Raptopoulou, C. P.; Pelecanou, M.; Papadopoulos, M.; Pirmettis, I.

(2009)

TY  - JOUR
AU  - Kyprianidou, P.
AU  - Tsoukalas, C.
AU  - Patsis, G.
AU  - Papagiannopoulou, D.
AU  - Nikolić, Nadežda S.
AU  - Janković, Drina
AU  - Đokić, Divna Đ.
AU  - Raptopoulou, C. P.
AU  - Pelecanou, M.
AU  - Papadopoulos, M.
AU  - Pirmettis, I.
PY  - 2009
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3811
AB  - Two rhenium(l) tricarbonyl complexes, with the monoanionic tridentate NSO type ligand, 4-(imidazolin-2-yl)-3-thiabutanoic acid and 4-(N-ethylimidazolin-2-yl)-3-thiabutanoic acid were synthesized and isolated in pure form. Both complexes were characterized by spectroscopic methods and elemental analysis. The solid-state structure of 4-(imidazolin-2-yl)-3-thiabutanoic acid and of both complexes was established by X-ray crystallography. The geometry about the rhenium is octahedral. The analogous technetium-99m complexes were also prepared quantitatively by the reaction of both ligands with the fac-[(99m)Tc(CO)(3)(H(2)O)(3)](+) synthon and their identity was established by chromatographic comparison to their rhenium congeners. (C) 2009 Elsevier Ltd. All rights reserved.
T2  - Polyhedron
T1  - Rhenium(I) and technetium-99m(I) fac-tricarbonyl complexes with 4-(imidazolin-2-yl)-3-thiabutanoic acid derivatives as tridentate ligands: Synthesis and structural characterization
VL  - 28
IS  - 15
SP  - 3171
EP  - 3176
DO  - 10.1016/j.poly.2009.04.005
ER  - 
@article{
author = "Kyprianidou, P. and Tsoukalas, C. and Patsis, G. and Papagiannopoulou, D. and Nikolić, Nadežda S. and Janković, Drina and Đokić, Divna Đ. and Raptopoulou, C. P. and Pelecanou, M. and Papadopoulos, M. and Pirmettis, I.",
year = "2009",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/3811",
abstract = "Two rhenium(l) tricarbonyl complexes, with the monoanionic tridentate NSO type ligand, 4-(imidazolin-2-yl)-3-thiabutanoic acid and 4-(N-ethylimidazolin-2-yl)-3-thiabutanoic acid were synthesized and isolated in pure form. Both complexes were characterized by spectroscopic methods and elemental analysis. The solid-state structure of 4-(imidazolin-2-yl)-3-thiabutanoic acid and of both complexes was established by X-ray crystallography. The geometry about the rhenium is octahedral. The analogous technetium-99m complexes were also prepared quantitatively by the reaction of both ligands with the fac-[(99m)Tc(CO)(3)(H(2)O)(3)](+) synthon and their identity was established by chromatographic comparison to their rhenium congeners. (C) 2009 Elsevier Ltd. All rights reserved.",
journal = "Polyhedron",
title = "Rhenium(I) and technetium-99m(I) fac-tricarbonyl complexes with 4-(imidazolin-2-yl)-3-thiabutanoic acid derivatives as tridentate ligands: Synthesis and structural characterization",
volume = "28",
number = "15",
pages = "3171-3176",
doi = "10.1016/j.poly.2009.04.005"
}
Kyprianidou, P., Tsoukalas, C., Patsis, G., Papagiannopoulou, D., Nikolić, N. S., Janković, D., Đokić, D. Đ., Raptopoulou, C. P., Pelecanou, M., Papadopoulos, M.,& Pirmettis, I. (2009). Rhenium(I) and technetium-99m(I) fac-tricarbonyl complexes with 4-(imidazolin-2-yl)-3-thiabutanoic acid derivatives as tridentate ligands: Synthesis and structural characterization.
Polyhedron, 28(15), 3171-3176.
https://doi.org/10.1016/j.poly.2009.04.005
Kyprianidou P, Tsoukalas C, Patsis G, Papagiannopoulou D, Nikolić NS, Janković D, Đokić DĐ, Raptopoulou CP, Pelecanou M, Papadopoulos M, Pirmettis I. Rhenium(I) and technetium-99m(I) fac-tricarbonyl complexes with 4-(imidazolin-2-yl)-3-thiabutanoic acid derivatives as tridentate ligands: Synthesis and structural characterization. Polyhedron. 2009;28(15):3171-3176
Kyprianidou P., Tsoukalas C., Patsis G., Papagiannopoulou D., Nikolić Nadežda S., Janković Drina, Đokić Divna Đ., Raptopoulou C. P., Pelecanou M., Papadopoulos M., Pirmettis I., "Rhenium(I) and technetium-99m(I) fac-tricarbonyl complexes with 4-(imidazolin-2-yl)-3-thiabutanoic acid derivatives as tridentate ligands: Synthesis and structural characterization" Polyhedron, 28, no. 15 (2009):3171-3176,
https://doi.org/10.1016/j.poly.2009.04.005 .
7
6
6

Preparation and In Vivo Evaluation of Y-90-Meso-Dimercaptosuccinic Acid (Y-90-DMSA) for Possible Therapeutic Use: Comparison with Tc-99m-DMSA

Đokić, Divna Đ.; Janković, Drina; Nikolić, Nadežda S.

(2009)

TY  - JOUR
AU  - Đokić, Divna Đ.
AU  - Janković, Drina
AU  - Nikolić, Nadežda S.
PY  - 2009
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3645
AB  - Introduction: The aim of this study was to find out if Y-90 could form a stabile complex with meso-2,3-dimercaptosuccinic acid (DMSA) and if Y-90-DMSA may have potential for tumor therapy in the palliative treatment of bone metastases. Methods: The preparing of Y-90-DMSA was carried out by varying experimental parameters, such as ligand concentration, pH, time, and temperature of the reaction, in order to maximize the labeling yield. Analysis of the complexes enclosed the radiochemical quality control (instant thin-layer chromatography, paper chromatography, and high-performance liquid chromatography), determination of pharmacokinetical parameters as well as biodistrbution study in health male Wistar rats. In vitro stability of the complexes was tested too. Results: Y-90-DMSA could be prepared in high yields ( GT 95%) under optimized conditions of reaction. Stability studies in saline and human serum in vitro showed no significant release of activity from the ligand over 24 hours and 10 days, respectively. The preliminary biodistribution results in rat at 2 hours indicated that Y-90-DMSA, at both pH levels, was significantly retained into bone. The uptake in the kidneys was lower for Y-90-DMSA at pH 8.0 then at pH 3.0. The retention in other organs was negligible. Conclusions: Y-90 complexes could be made with ease with DMSA. Y-90-DMSA was obtained in good yield and was found to be very stable. A promising biodistribution result of this complex pointed at potential in the palliative treatment of bone metastases.
T2  - Cancer Biotherapy and Radiopharmaceuticals
T1  - Preparation and In Vivo Evaluation of Y-90-Meso-Dimercaptosuccinic Acid (Y-90-DMSA) for Possible Therapeutic Use: Comparison with Tc-99m-DMSA
VL  - 24
IS  - 1
SP  - 129
EP  - 136
DO  - 10.1089/cbr.2008.0499
ER  - 
@article{
author = "Đokić, Divna Đ. and Janković, Drina and Nikolić, Nadežda S.",
year = "2009",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/3645",
abstract = "Introduction: The aim of this study was to find out if Y-90 could form a stabile complex with meso-2,3-dimercaptosuccinic acid (DMSA) and if Y-90-DMSA may have potential for tumor therapy in the palliative treatment of bone metastases. Methods: The preparing of Y-90-DMSA was carried out by varying experimental parameters, such as ligand concentration, pH, time, and temperature of the reaction, in order to maximize the labeling yield. Analysis of the complexes enclosed the radiochemical quality control (instant thin-layer chromatography, paper chromatography, and high-performance liquid chromatography), determination of pharmacokinetical parameters as well as biodistrbution study in health male Wistar rats. In vitro stability of the complexes was tested too. Results: Y-90-DMSA could be prepared in high yields ( GT 95%) under optimized conditions of reaction. Stability studies in saline and human serum in vitro showed no significant release of activity from the ligand over 24 hours and 10 days, respectively. The preliminary biodistribution results in rat at 2 hours indicated that Y-90-DMSA, at both pH levels, was significantly retained into bone. The uptake in the kidneys was lower for Y-90-DMSA at pH 8.0 then at pH 3.0. The retention in other organs was negligible. Conclusions: Y-90 complexes could be made with ease with DMSA. Y-90-DMSA was obtained in good yield and was found to be very stable. A promising biodistribution result of this complex pointed at potential in the palliative treatment of bone metastases.",
journal = "Cancer Biotherapy and Radiopharmaceuticals",
title = "Preparation and In Vivo Evaluation of Y-90-Meso-Dimercaptosuccinic Acid (Y-90-DMSA) for Possible Therapeutic Use: Comparison with Tc-99m-DMSA",
volume = "24",
number = "1",
pages = "129-136",
doi = "10.1089/cbr.2008.0499"
}
Đokić, D. Đ., Janković, D.,& Nikolić, N. S. (2009). Preparation and In Vivo Evaluation of Y-90-Meso-Dimercaptosuccinic Acid (Y-90-DMSA) for Possible Therapeutic Use: Comparison with Tc-99m-DMSA.
Cancer Biotherapy and Radiopharmaceuticals, 24(1), 129-136.
https://doi.org/10.1089/cbr.2008.0499
Đokić DĐ, Janković D, Nikolić NS. Preparation and In Vivo Evaluation of Y-90-Meso-Dimercaptosuccinic Acid (Y-90-DMSA) for Possible Therapeutic Use: Comparison with Tc-99m-DMSA. Cancer Biotherapy and Radiopharmaceuticals. 2009;24(1):129-136
Đokić Divna Đ., Janković Drina, Nikolić Nadežda S., "Preparation and In Vivo Evaluation of Y-90-Meso-Dimercaptosuccinic Acid (Y-90-DMSA) for Possible Therapeutic Use: Comparison with Tc-99m-DMSA" Cancer Biotherapy and Radiopharmaceuticals, 24, no. 1 (2009):129-136,
https://doi.org/10.1089/cbr.2008.0499 .
6
4
4

Technetium-99m in Production and Use

Vučina, Jurij L.; Nikolić, Nadežda S.; Petrović, Đokica

(2009)

TY  - JOUR
AU  - Vučina, Jurij L.
AU  - Nikolić, Nadežda S.
AU  - Petrović, Đokica
PY  - 2009
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3687
AB  - Several types of generators have been developed for the production of (99m)Tc. Due to its excelent performances, the chromatographic type, based on the fission-produced (99)Mo sorbed in alumina, is predominant. Technetium-99m is obtained in the form of sodium pertechnetate-(99m)Tc. However, due to the known disadvantages of the production of (n, f)(99)Mo, attempts are made to avoid uranium fission. The technologies based on (n, gamma)(99)Mo (sublimation, extraction, gel) are, with the exception of the gel generator, of limited importance. Certain nuclear reactions in cyclotrons can produce (99)Mo (or directly (99m)Tc) but the obtained results are still not satisfying. Technetium-99m is used in the form of radiopharmaceuticals which are prepared by addition of (99m)Tc-eluate to the inactive components comprised in the cold kits. The chromatographic (n, f)(99)Mo/(99m)Tc generator and several (99m)Tc-radiopharmaceuticals have been developed and are regularly produced in the Vinca Institute of Nuclear Sciences (Laboratory for Radioisotopes).
T2  - Nuclear technology and radiation protection
T1  - Technetium-99m in Production and Use
VL  - 24
IS  - 1
SP  - 68
EP  - 73
DO  - 10.2298/NTRP0901068V
ER  - 
@article{
author = "Vučina, Jurij L. and Nikolić, Nadežda S. and Petrović, Đokica",
year = "2009",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/3687",
abstract = "Several types of generators have been developed for the production of (99m)Tc. Due to its excelent performances, the chromatographic type, based on the fission-produced (99)Mo sorbed in alumina, is predominant. Technetium-99m is obtained in the form of sodium pertechnetate-(99m)Tc. However, due to the known disadvantages of the production of (n, f)(99)Mo, attempts are made to avoid uranium fission. The technologies based on (n, gamma)(99)Mo (sublimation, extraction, gel) are, with the exception of the gel generator, of limited importance. Certain nuclear reactions in cyclotrons can produce (99)Mo (or directly (99m)Tc) but the obtained results are still not satisfying. Technetium-99m is used in the form of radiopharmaceuticals which are prepared by addition of (99m)Tc-eluate to the inactive components comprised in the cold kits. The chromatographic (n, f)(99)Mo/(99m)Tc generator and several (99m)Tc-radiopharmaceuticals have been developed and are regularly produced in the Vinca Institute of Nuclear Sciences (Laboratory for Radioisotopes).",
journal = "Nuclear technology and radiation protection",
title = "Technetium-99m in Production and Use",
volume = "24",
number = "1",
pages = "68-73",
doi = "10.2298/NTRP0901068V"
}
Vučina, J. L., Nikolić, N. S.,& Petrović, Đ. (2009). Technetium-99m in Production and Use.
Nuclear technology and radiation protection, 24(1), 68-73.
https://doi.org/10.2298/NTRP0901068V
Vučina JL, Nikolić NS, Petrović Đ. Technetium-99m in Production and Use. Nuclear technology and radiation protection. 2009;24(1):68-73
Vučina Jurij L., Nikolić Nadežda S., Petrović Đokica, "Technetium-99m in Production and Use" Nuclear technology and radiation protection, 24, no. 1 (2009):68-73,
https://doi.org/10.2298/NTRP0901068V .
2
2
2

Opposite effects of nanocrystalline fullerene (C-60) on tumour cell growth in vitro and in vivo and a possible role of immunosupression in the cancer-promoting activity of C-60

Zogovic, Nevena S.; Nikolić, Nadežda S.; Vranješ-Đurić, Sanja; Harhaji, Ljubica M.; Vucicevic, Ljubica M.; Janjetović, Kristina D.; Misirkić, Maja S.; Todorović-Marković, Biljana; Marković, Zoran M.; Milonjić, Slobodan K.; Trajković, Vladimir S.

(2009)

TY  - JOUR
AU  - Zogovic, Nevena S.
AU  - Nikolić, Nadežda S.
AU  - Vranješ-Đurić, Sanja
AU  - Harhaji, Ljubica M.
AU  - Vucicevic, Ljubica M.
AU  - Janjetović, Kristina D.
AU  - Misirkić, Maja S.
AU  - Todorović-Marković, Biljana
AU  - Marković, Zoran M.
AU  - Milonjić, Slobodan K.
AU  - Trajković, Vladimir S.
PY  - 2009
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3830
AB  - In the present study, we compared the effects of nanocrystalline fullerene suspension (nanoC(60)) on tumour cell growth in vitro and in vivo. NanoC(60) suspension was prepared by solvent exchange using tetrahydrofuran to dissolve C-60. In vitro, nanoC(60) caused oxidative stress, mitochondrial depolarization and caspase activation, leading to apoptotic and necrotic death in mouse B16 melanoma cells. Bio-distribution studies demonstrated that intraperitoneally injected radiolabeled (I-125) nanoC(60) readily accumulated in the tumour tissue of mice subcutaneously inoculated with B16 cells. However, intraperitoneal administration of nanoC(60) over the course of two weeks starting from melanoma cell implantation not only failed to reduce, but significantly augmented turnout growth. The tumour-promoting effect of nanoC(60) was accompanied by a significant increase in splenocyte production of the immunoregulatory free radical nitric oxide (NO), as well as by a reduction in splenocyte proliferative responses to T- and B-cell mitogens ConcanavalinA and bacterial lipopolysaccharide, respectively. A negative correlation between NO production and splenocyte proliferation indicated a possible role of NO in reducing the proliferation of splenocytes from nanoC(60)-injected mice. These data demonstrate that nanoC(60), in contrast to its potent anticancer activity in vitro, can potentiate tumour growth in vivo, possibly by causing NO-dependent suppression of anticancer immune response. (C) 2009 Elsevier Ltd. All rights reserved.
T2  - Biomaterials
T1  - Opposite effects of nanocrystalline fullerene (C-60) on tumour cell growth in vitro and in vivo and a possible role of immunosupression in the cancer-promoting activity of C-60
VL  - 30
IS  - 36
SP  - 6940
EP  - 6946
DO  - 10.1016/j.biomaterials.2009.09.007
ER  - 
@article{
author = "Zogovic, Nevena S. and Nikolić, Nadežda S. and Vranješ-Đurić, Sanja and Harhaji, Ljubica M. and Vucicevic, Ljubica M. and Janjetović, Kristina D. and Misirkić, Maja S. and Todorović-Marković, Biljana and Marković, Zoran M. and Milonjić, Slobodan K. and Trajković, Vladimir S.",
year = "2009",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/3830",
abstract = "In the present study, we compared the effects of nanocrystalline fullerene suspension (nanoC(60)) on tumour cell growth in vitro and in vivo. NanoC(60) suspension was prepared by solvent exchange using tetrahydrofuran to dissolve C-60. In vitro, nanoC(60) caused oxidative stress, mitochondrial depolarization and caspase activation, leading to apoptotic and necrotic death in mouse B16 melanoma cells. Bio-distribution studies demonstrated that intraperitoneally injected radiolabeled (I-125) nanoC(60) readily accumulated in the tumour tissue of mice subcutaneously inoculated with B16 cells. However, intraperitoneal administration of nanoC(60) over the course of two weeks starting from melanoma cell implantation not only failed to reduce, but significantly augmented turnout growth. The tumour-promoting effect of nanoC(60) was accompanied by a significant increase in splenocyte production of the immunoregulatory free radical nitric oxide (NO), as well as by a reduction in splenocyte proliferative responses to T- and B-cell mitogens ConcanavalinA and bacterial lipopolysaccharide, respectively. A negative correlation between NO production and splenocyte proliferation indicated a possible role of NO in reducing the proliferation of splenocytes from nanoC(60)-injected mice. These data demonstrate that nanoC(60), in contrast to its potent anticancer activity in vitro, can potentiate tumour growth in vivo, possibly by causing NO-dependent suppression of anticancer immune response. (C) 2009 Elsevier Ltd. All rights reserved.",
journal = "Biomaterials",
title = "Opposite effects of nanocrystalline fullerene (C-60) on tumour cell growth in vitro and in vivo and a possible role of immunosupression in the cancer-promoting activity of C-60",
volume = "30",
number = "36",
pages = "6940-6946",
doi = "10.1016/j.biomaterials.2009.09.007"
}
Zogovic, N. S., Nikolić, N. S., Vranješ-Đurić, S., Harhaji, L. M., Vucicevic, L. M., Janjetović, K. D., Misirkić, M. S., Todorović-Marković, B., Marković, Z. M., Milonjić, S. K.,& Trajković, V. S. (2009). Opposite effects of nanocrystalline fullerene (C-60) on tumour cell growth in vitro and in vivo and a possible role of immunosupression in the cancer-promoting activity of C-60.
Biomaterials, 30(36), 6940-6946.
https://doi.org/10.1016/j.biomaterials.2009.09.007
Zogovic NS, Nikolić NS, Vranješ-Đurić S, Harhaji LM, Vucicevic LM, Janjetović KD, Misirkić MS, Todorović-Marković B, Marković ZM, Milonjić SK, Trajković VS. Opposite effects of nanocrystalline fullerene (C-60) on tumour cell growth in vitro and in vivo and a possible role of immunosupression in the cancer-promoting activity of C-60. Biomaterials. 2009;30(36):6940-6946
Zogovic Nevena S., Nikolić Nadežda S., Vranješ-Đurić Sanja, Harhaji Ljubica M., Vucicevic Ljubica M., Janjetović Kristina D., Misirkić Maja S., Todorović-Marković Biljana, Marković Zoran M., Milonjić Slobodan K., Trajković Vladimir S., "Opposite effects of nanocrystalline fullerene (C-60) on tumour cell growth in vitro and in vivo and a possible role of immunosupression in the cancer-promoting activity of C-60" Biomaterials, 30, no. 36 (2009):6940-6946,
https://doi.org/10.1016/j.biomaterials.2009.09.007 .
37
36
40

Preparation and biodistribution of radiolabeled fullerene C-60 nanocrystals

Nikolić, Nadežda S.; Vranješ-Đurić, Sanja; Janković, Drina; Dokic, Divna; Mirković, Marija D.; Bibić, Nataša M.; Trajković, Vladimir S.

(2009)

TY  - JOUR
AU  - Nikolić, Nadežda S.
AU  - Vranješ-Đurić, Sanja
AU  - Janković, Drina
AU  - Dokic, Divna
AU  - Mirković, Marija D.
AU  - Bibić, Nataša M.
AU  - Trajković, Vladimir S.
PY  - 2009
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3777
AB  - The present study describes for the first time a procedure for the radiolabeling of fullerene (C-60) nanocrystals (nanoC(60)) with Na I-125, as well as the biodistribution of radiolabeled nanoC(60) (I-125-nanoC(60)). The solvent exchange method with tetrahydrofuran was used to make colloidal water suspensions of radiolabeled nanoC(60) particles. The radiolabeling procedure with the addition of Na I-125 to tetrahydrofuran during dissolution of C-60 gave a higher radiochemical yield of radiolabeled nanoC(60) particles in comparison to the second option, in which Na I-125 was added after C-60 was dissolved. Using photon correlation spectroscopy and transmission electron microscopy, I-125-nanoC(60) particles were found to have a crystalline structure and a mean diameter of 200-250 nm. The I-125-nanoC(60) had a particularly high affinity for human serum albumin, displaying 95% binding efficiency after 1 h. Biodistribution studies of I-125-nanoC(60) in rats indicated significant differences in tissue accumulation of I-125-nanoC(60) and the radioactive tracer Na I-125. The higher accumulation of radiolabeled nanoC(60) was observed in liver and spleen, while accumulation in thyroid, stomach, lungs and intestines was significantly lower in comparison to Na I-125. In addition to being useful for testing the biological distribution of nanoC(60), the described radiolabeling procedure might have possible applications in cancer radiotherapy.
T2  - Nanotechnology
T1  - Preparation and biodistribution of radiolabeled fullerene C-60 nanocrystals
VL  - 20
IS  - 38
DO  - 10.1088/0957-4484/20/38/385102
ER  - 
@article{
author = "Nikolić, Nadežda S. and Vranješ-Đurić, Sanja and Janković, Drina and Dokic, Divna and Mirković, Marija D. and Bibić, Nataša M. and Trajković, Vladimir S.",
year = "2009",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/3777",
abstract = "The present study describes for the first time a procedure for the radiolabeling of fullerene (C-60) nanocrystals (nanoC(60)) with Na I-125, as well as the biodistribution of radiolabeled nanoC(60) (I-125-nanoC(60)). The solvent exchange method with tetrahydrofuran was used to make colloidal water suspensions of radiolabeled nanoC(60) particles. The radiolabeling procedure with the addition of Na I-125 to tetrahydrofuran during dissolution of C-60 gave a higher radiochemical yield of radiolabeled nanoC(60) particles in comparison to the second option, in which Na I-125 was added after C-60 was dissolved. Using photon correlation spectroscopy and transmission electron microscopy, I-125-nanoC(60) particles were found to have a crystalline structure and a mean diameter of 200-250 nm. The I-125-nanoC(60) had a particularly high affinity for human serum albumin, displaying 95% binding efficiency after 1 h. Biodistribution studies of I-125-nanoC(60) in rats indicated significant differences in tissue accumulation of I-125-nanoC(60) and the radioactive tracer Na I-125. The higher accumulation of radiolabeled nanoC(60) was observed in liver and spleen, while accumulation in thyroid, stomach, lungs and intestines was significantly lower in comparison to Na I-125. In addition to being useful for testing the biological distribution of nanoC(60), the described radiolabeling procedure might have possible applications in cancer radiotherapy.",
journal = "Nanotechnology",
title = "Preparation and biodistribution of radiolabeled fullerene C-60 nanocrystals",
volume = "20",
number = "38",
doi = "10.1088/0957-4484/20/38/385102"
}
Nikolić, N. S., Vranješ-Đurić, S., Janković, D., Dokic, D., Mirković, M. D., Bibić, N. M.,& Trajković, V. S. (2009). Preparation and biodistribution of radiolabeled fullerene C-60 nanocrystals.
Nanotechnology, 20(38).
https://doi.org/10.1088/0957-4484/20/38/385102
Nikolić NS, Vranješ-Đurić S, Janković D, Dokic D, Mirković MD, Bibić NM, Trajković VS. Preparation and biodistribution of radiolabeled fullerene C-60 nanocrystals. Nanotechnology. 2009;20(38)
Nikolić Nadežda S., Vranješ-Đurić Sanja, Janković Drina, Dokic Divna, Mirković Marija D., Bibić Nataša M., Trajković Vladimir S., "Preparation and biodistribution of radiolabeled fullerene C-60 nanocrystals" Nanotechnology, 20, no. 38 (2009),
https://doi.org/10.1088/0957-4484/20/38/385102 .
32
27
35

Particle size analysis: (90)Y and (99m)Tc-labelled colloids

Janković, Drina; Maksin, Tatjana N.; Đokić, Divna Đ.; Milonjić, Slobodan K.; Nikolić, Nadežda S.; Mirković, Marija D.; Vranješ-Đurić, Sanja

(2008)

TY  - JOUR
AU  - Janković, Drina
AU  - Maksin, Tatjana N.
AU  - Đokić, Divna Đ.
AU  - Milonjić, Slobodan K.
AU  - Nikolić, Nadežda S.
AU  - Mirković, Marija D.
AU  - Vranješ-Đurić, Sanja
PY  - 2008
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3578
AB  - Colloidal particle size is an important characteristic to consider when choosing a radiopharmaceutical for diagnosis and therapeutic purposes in nuclear medicine. Photon correlation spectroscopy (PCS) and transmission electron microscopy (TEM) were used to determine the particle-size distribution of (90)Y- and (99m)Tc-labelled antimony trisulfide (Sb(2)S(3)) and tin colloids (Sn-colloid). (90)Y-Sb(2)S(3) and (99m)Tc-Sb(2)S(3) were found to have a diameter of 28.92 +/- 0.14 and 35.61 +/- 0.11 nm, respectively, by PCS. By TEM, (90)Y-Sb(2)S(3) particles were measured to be 14.33 +/- 0.09 nm. (90)Y-labelled Sn colloid were found to exist with a d(v(max1)) of 805 nm and a d(v(max2)) of 2590 nm, by PCS, whereas (99m)Tc-Sn colloid was shown to have more than 80% of radioactive particles of approximately 910 nm by PCS. For (90)Y-labelled Sb(2)S(3) and Sn colloid, a comparison of TEM and PCS indicates that these techniques found significantly different mean diameters. TEM has an excellent resolution necessary for radiocolloid particle-sizing analysis, and it is a desirable size-measuring technique because it is more reliable than PCS.
T2  - Journal of Microscopy, Oxford
T1  - Particle size analysis: (90)Y and (99m)Tc-labelled colloids
VL  - 232
IS  - 3
SP  - 601
EP  - 604
DO  - 10.1111/j.1365-2818.2008.02124.x
ER  - 
@article{
author = "Janković, Drina and Maksin, Tatjana N. and Đokić, Divna Đ. and Milonjić, Slobodan K. and Nikolić, Nadežda S. and Mirković, Marija D. and Vranješ-Đurić, Sanja",
year = "2008",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/3578",
abstract = "Colloidal particle size is an important characteristic to consider when choosing a radiopharmaceutical for diagnosis and therapeutic purposes in nuclear medicine. Photon correlation spectroscopy (PCS) and transmission electron microscopy (TEM) were used to determine the particle-size distribution of (90)Y- and (99m)Tc-labelled antimony trisulfide (Sb(2)S(3)) and tin colloids (Sn-colloid). (90)Y-Sb(2)S(3) and (99m)Tc-Sb(2)S(3) were found to have a diameter of 28.92 +/- 0.14 and 35.61 +/- 0.11 nm, respectively, by PCS. By TEM, (90)Y-Sb(2)S(3) particles were measured to be 14.33 +/- 0.09 nm. (90)Y-labelled Sn colloid were found to exist with a d(v(max1)) of 805 nm and a d(v(max2)) of 2590 nm, by PCS, whereas (99m)Tc-Sn colloid was shown to have more than 80% of radioactive particles of approximately 910 nm by PCS. For (90)Y-labelled Sb(2)S(3) and Sn colloid, a comparison of TEM and PCS indicates that these techniques found significantly different mean diameters. TEM has an excellent resolution necessary for radiocolloid particle-sizing analysis, and it is a desirable size-measuring technique because it is more reliable than PCS.",
journal = "Journal of Microscopy, Oxford",
title = "Particle size analysis: (90)Y and (99m)Tc-labelled colloids",
volume = "232",
number = "3",
pages = "601-604",
doi = "10.1111/j.1365-2818.2008.02124.x"
}
Janković, D., Maksin, T. N., Đokić, D. Đ., Milonjić, S. K., Nikolić, N. S., Mirković, M. D.,& Vranješ-Đurić, S. (2008). Particle size analysis: (90)Y and (99m)Tc-labelled colloids.
Journal of Microscopy, Oxford, 232(3), 601-604.
https://doi.org/10.1111/j.1365-2818.2008.02124.x
Janković D, Maksin TN, Đokić DĐ, Milonjić SK, Nikolić NS, Mirković MD, Vranješ-Đurić S. Particle size analysis: (90)Y and (99m)Tc-labelled colloids. Journal of Microscopy, Oxford. 2008;232(3):601-604
Janković Drina, Maksin Tatjana N., Đokić Divna Đ., Milonjić Slobodan K., Nikolić Nadežda S., Mirković Marija D., Vranješ-Đurić Sanja, "Particle size analysis: (90)Y and (99m)Tc-labelled colloids" Journal of Microscopy, Oxford, 232, no. 3 (2008):601-604,
https://doi.org/10.1111/j.1365-2818.2008.02124.x .
12
10
13

Labeling, characterization, and in vivo localization of a new Y-90-based phosphonate chelate 2,3-dicarboxypropane-1,1-diphosphonic acid for the treatment of bone metastases: Comparison with Tc-99m-DPD complex

Đokić, Divna Đ.; Janković, Drina; Nikolić, Nadežda S.

(2008)

TY  - JOUR
AU  - Đokić, Divna Đ.
AU  - Janković, Drina
AU  - Nikolić, Nadežda S.
PY  - 2008
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3240
AB  - The goal of this investigation was to examine the possibilities for yttrium-90-labeling of the 2,3-dicarboxypropane-1,1-diphosphonic acid (DPD), which is currently labeled with technetium-99m and as a Tc-99m-DPD clinically used as bone imaging agent. Analysis of the complex enclosed the radiochemical quality control methods, biodistribution studies, as well as the determination of pharmacokinetic parameters. The biological behavior of complexes Y-90-DPD, Tc-99m-DPD and Y-90-labeled DPD-kit formulation [Y-90-(Sn)-DPD] in animal model was compared. The labeling conditions were standardized to give the maximum yield, which ranged between 93% and 98%. The examined Y-90 complex could be easily prepared, with an outstanding yield and was also found to be very stable for at least 10 h after Y-90-labeling. Protein binding value was 4.6 +/- 0.7% for Y-90-DPD complex and the complex possess a hydrophilic character. The satisfactory results of Y-90- DPD biodistribution in healthy test animals were obtained; the uptake in the bone was 11-13% ID/g after 24 h depending on the pH value during the preparation. With high skeletal uptake, a minimum uptake in soft tissues and rapid blood clearance the Y-90-DPD complex proved to be an excellent candidate for targeting tumor therapy. (C) 2008 Elsevier Ltd. All rights reserved.
T2  - Bioorganic and Medicinal Chemistry
T1  - Labeling, characterization, and in vivo localization of a new Y-90-based phosphonate chelate 2,3-dicarboxypropane-1,1-diphosphonic acid for the treatment of bone metastases: Comparison with Tc-99m-DPD complex
VL  - 16
IS  - 8
SP  - 4457
EP  - 4465
DO  - 10.1016/j.bmc.2008.02.062
ER  - 
@article{
author = "Đokić, Divna Đ. and Janković, Drina and Nikolić, Nadežda S.",
year = "2008",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/3240",
abstract = "The goal of this investigation was to examine the possibilities for yttrium-90-labeling of the 2,3-dicarboxypropane-1,1-diphosphonic acid (DPD), which is currently labeled with technetium-99m and as a Tc-99m-DPD clinically used as bone imaging agent. Analysis of the complex enclosed the radiochemical quality control methods, biodistribution studies, as well as the determination of pharmacokinetic parameters. The biological behavior of complexes Y-90-DPD, Tc-99m-DPD and Y-90-labeled DPD-kit formulation [Y-90-(Sn)-DPD] in animal model was compared. The labeling conditions were standardized to give the maximum yield, which ranged between 93% and 98%. The examined Y-90 complex could be easily prepared, with an outstanding yield and was also found to be very stable for at least 10 h after Y-90-labeling. Protein binding value was 4.6 +/- 0.7% for Y-90-DPD complex and the complex possess a hydrophilic character. The satisfactory results of Y-90- DPD biodistribution in healthy test animals were obtained; the uptake in the bone was 11-13% ID/g after 24 h depending on the pH value during the preparation. With high skeletal uptake, a minimum uptake in soft tissues and rapid blood clearance the Y-90-DPD complex proved to be an excellent candidate for targeting tumor therapy. (C) 2008 Elsevier Ltd. All rights reserved.",
journal = "Bioorganic and Medicinal Chemistry",
title = "Labeling, characterization, and in vivo localization of a new Y-90-based phosphonate chelate 2,3-dicarboxypropane-1,1-diphosphonic acid for the treatment of bone metastases: Comparison with Tc-99m-DPD complex",
volume = "16",
number = "8",
pages = "4457-4465",
doi = "10.1016/j.bmc.2008.02.062"
}
Đokić, D. Đ., Janković, D.,& Nikolić, N. S. (2008). Labeling, characterization, and in vivo localization of a new Y-90-based phosphonate chelate 2,3-dicarboxypropane-1,1-diphosphonic acid for the treatment of bone metastases: Comparison with Tc-99m-DPD complex.
Bioorganic and Medicinal Chemistry, 16(8), 4457-4465.
https://doi.org/10.1016/j.bmc.2008.02.062
Đokić DĐ, Janković D, Nikolić NS. Labeling, characterization, and in vivo localization of a new Y-90-based phosphonate chelate 2,3-dicarboxypropane-1,1-diphosphonic acid for the treatment of bone metastases: Comparison with Tc-99m-DPD complex. Bioorganic and Medicinal Chemistry. 2008;16(8):4457-4465
Đokić Divna Đ., Janković Drina, Nikolić Nadežda S., "Labeling, characterization, and in vivo localization of a new Y-90-based phosphonate chelate 2,3-dicarboxypropane-1,1-diphosphonic acid for the treatment of bone metastases: Comparison with Tc-99m-DPD complex" Bioorganic and Medicinal Chemistry, 16, no. 8 (2008):4457-4465,
https://doi.org/10.1016/j.bmc.2008.02.062 .
12
12
10

Modulation of tumor necrosis factor-mediated cell death by fullerenes

Harhaji, Ljubica M.; Isaković, Aleksandra J.; Vucicevic, Ljubica; Janjetović, Kristina D.; Misirkić, Maja; Marković, Zoran M.; Todorović-Marković, Biljana; Nikolić, Nadežda S.; Vranješ-Đurić, Sanja; Nikolić, Zoran M.; Trajković, Vladimir S.

(2008)

TY  - JOUR
AU  - Harhaji, Ljubica M.
AU  - Isaković, Aleksandra J.
AU  - Vucicevic, Ljubica
AU  - Janjetović, Kristina D.
AU  - Misirkić, Maja
AU  - Marković, Zoran M.
AU  - Todorović-Marković, Biljana
AU  - Nikolić, Nadežda S.
AU  - Vranješ-Đurić, Sanja
AU  - Nikolić, Zoran M.
AU  - Trajković, Vladimir S.
PY  - 2008
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3445
AB  - Purpose. The fullerene (C-60/C-70 mixture-C-60/70) nanocrystalline suspension prepared by solvent exchange method using tetrahydrofyran (THF/nC(60/70)) and polyhydroxylated C-60/70 [C-60/70(OH)(n)] were compared for their ability to modulate cytotoxicity of the proinflammatory cytokine tumor necrosis factor (TNT). Materials and Methods. TNF-induced cytotoxicity was assessed in L929 fibrosarcoma cells by crystal violet assay. The type of cell death (apoptosis/necrosis), production of reactive oxygen species, mitochondrial depolarization and caspase activation were determined by flow cytometry using the appropriate reporter dyes. Results. THF/nC(60/70) augmented, while C-60/70(OH)(n) reduced the cytotoxicity of TNF. The numbers of cells undergoing apoptosis/necrosis, as well as of those displaying the activation of apoptosis-inducing enzymes of caspase family, were respectively increased or reduced by THF/nC(60/70) or C-60/70(OH)(n). The antioxidant N-acetylcysteine and mitochondrial permeability transition inhibitor cyclosporin A each partly blocked the cytotoxic action of TNF, indicating the involvement of oxidative stress and mitochondrial dysfunction in the TNF cytotoxicity. Accordingly, THF/nC(60/70) or C-60/70(OH)(n) potentiated or suppressed, respectively, TNF-triggered oxidative stress and mitochondrial depolarization. Conclusion. The ability of different fullerene preparations to modulate TNF-induced oxidative stress and subsequent cell death suggests their potential value in the TNF-based cancer therapy or prevention of TNF-dependent tissue damage.
T2  - Pharmaceutical Research
T1  - Modulation of tumor necrosis factor-mediated cell death by fullerenes
VL  - 25
IS  - 6
SP  - 1365
EP  - 1376
DO  - 10.1007/s11095-007-9486-y
ER  - 
@article{
author = "Harhaji, Ljubica M. and Isaković, Aleksandra J. and Vucicevic, Ljubica and Janjetović, Kristina D. and Misirkić, Maja and Marković, Zoran M. and Todorović-Marković, Biljana and Nikolić, Nadežda S. and Vranješ-Đurić, Sanja and Nikolić, Zoran M. and Trajković, Vladimir S.",
year = "2008",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/3445",
abstract = "Purpose. The fullerene (C-60/C-70 mixture-C-60/70) nanocrystalline suspension prepared by solvent exchange method using tetrahydrofyran (THF/nC(60/70)) and polyhydroxylated C-60/70 [C-60/70(OH)(n)] were compared for their ability to modulate cytotoxicity of the proinflammatory cytokine tumor necrosis factor (TNT). Materials and Methods. TNF-induced cytotoxicity was assessed in L929 fibrosarcoma cells by crystal violet assay. The type of cell death (apoptosis/necrosis), production of reactive oxygen species, mitochondrial depolarization and caspase activation were determined by flow cytometry using the appropriate reporter dyes. Results. THF/nC(60/70) augmented, while C-60/70(OH)(n) reduced the cytotoxicity of TNF. The numbers of cells undergoing apoptosis/necrosis, as well as of those displaying the activation of apoptosis-inducing enzymes of caspase family, were respectively increased or reduced by THF/nC(60/70) or C-60/70(OH)(n). The antioxidant N-acetylcysteine and mitochondrial permeability transition inhibitor cyclosporin A each partly blocked the cytotoxic action of TNF, indicating the involvement of oxidative stress and mitochondrial dysfunction in the TNF cytotoxicity. Accordingly, THF/nC(60/70) or C-60/70(OH)(n) potentiated or suppressed, respectively, TNF-triggered oxidative stress and mitochondrial depolarization. Conclusion. The ability of different fullerene preparations to modulate TNF-induced oxidative stress and subsequent cell death suggests their potential value in the TNF-based cancer therapy or prevention of TNF-dependent tissue damage.",
journal = "Pharmaceutical Research",
title = "Modulation of tumor necrosis factor-mediated cell death by fullerenes",
volume = "25",
number = "6",
pages = "1365-1376",
doi = "10.1007/s11095-007-9486-y"
}
Harhaji, L. M., Isaković, A. J., Vucicevic, L., Janjetović, K. D., Misirkić, M., Marković, Z. M., Todorović-Marković, B., Nikolić, N. S., Vranješ-Đurić, S., Nikolić, Z. M.,& Trajković, V. S. (2008). Modulation of tumor necrosis factor-mediated cell death by fullerenes.
Pharmaceutical Research, 25(6), 1365-1376.
https://doi.org/10.1007/s11095-007-9486-y
Harhaji LM, Isaković AJ, Vucicevic L, Janjetović KD, Misirkić M, Marković ZM, Todorović-Marković B, Nikolić NS, Vranješ-Đurić S, Nikolić ZM, Trajković VS. Modulation of tumor necrosis factor-mediated cell death by fullerenes. Pharmaceutical Research. 2008;25(6):1365-1376
Harhaji Ljubica M., Isaković Aleksandra J., Vucicevic Ljubica, Janjetović Kristina D., Misirkić Maja, Marković Zoran M., Todorović-Marković Biljana, Nikolić Nadežda S., Vranješ-Đurić Sanja, Nikolić Zoran M., Trajković Vladimir S., "Modulation of tumor necrosis factor-mediated cell death by fullerenes" Pharmaceutical Research, 25, no. 6 (2008):1365-1376,
https://doi.org/10.1007/s11095-007-9486-y .
19
18
23

The mechanism of cell-damaging reactive oxygen generation by colloidal fullerenes

Marković, Zoran M.; Todorović-Marković, Biljana; Kleut, Duška; Nikolić, Nadežda S.; Vranješ-Đurić, Sanja; Misirkić, Maja; Vucicevic, Ljubica; Janjetović, Kristina D.; Isaković, Aleksandra J.; Harhaji, Ljubica M.; Babić-Stojić Branka S.; Dramićanin, Miroslav; Trajković, Vladimir S.

(2007)

TY  - JOUR
AU  - Marković, Zoran M.
AU  - Todorović-Marković, Biljana
AU  - Kleut, Duška
AU  - Nikolić, Nadežda S.
AU  - Vranješ-Đurić, Sanja
AU  - Misirkić, Maja
AU  - Vucicevic, Ljubica
AU  - Janjetović, Kristina D.
AU  - Isaković, Aleksandra J.
AU  - Harhaji, Ljubica M.
AU  - Babić-Stojić Branka S.
AU  - Dramićanin, Miroslav
AU  - Trajković, Vladimir S.
PY  - 2007
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3322
AB  - Because of the ability to induce cell death in certain conditions, the fullerenes (C-60) are potential anticancer and toxic agents. The colloidal suspension of crystalline C-60 (nano-C-60, nC(60)) is extremely toxic, but the mechanisms of its cytotoxicity are not completely understood. By combining experimental analysis and mathematical modelling, we investigate the requirements for the reactive oxygen species (ROS)-mediated cytotoxicity of different nC(60) suspensions, prepared by solvent exchange method in tetrahydrofuran (THF/nC(60)) and ethanol (EtOH/nC(60)), or by extended mixing in water (aqu/nC(60)). With regard to their capacity to generate ROS and cause mitochondrial depolarization followed by necrotic cell death, the nC(60) suspensions are ranked in the following order: THF/nC(60) GT EtOH/nC(60) GT aqu/nC(60). Mathematical modelling of singlet oxygen (O-1(2)) generation indicates that the O-1(2)-quenching power (THF/nC(60) LT EtOH/nC(60) LT aqU/nC(60)) of the solvent intercalated in the fullerene crystals determines their ability to produce ROS and cause cell damage. These data could. have important implications for toxicology and biomedical application of colloidal fullerenes. (C) 2007 Elsevier Ltd. All rights reserved.
T2  - Biomaterials
T1  - The mechanism of cell-damaging reactive oxygen generation by colloidal fullerenes
VL  - 28
IS  - 36
SP  - 5437
EP  - 5448
DO  - 10.1016/j.biomaterials.2007.09.002
ER  - 
@article{
author = "Marković, Zoran M. and Todorović-Marković, Biljana and Kleut, Duška and Nikolić, Nadežda S. and Vranješ-Đurić, Sanja and Misirkić, Maja and Vucicevic, Ljubica and Janjetović, Kristina D. and Isaković, Aleksandra J. and Harhaji, Ljubica M. and Babić-Stojić Branka S. and Dramićanin, Miroslav and Trajković, Vladimir S.",
year = "2007",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/3322",
abstract = "Because of the ability to induce cell death in certain conditions, the fullerenes (C-60) are potential anticancer and toxic agents. The colloidal suspension of crystalline C-60 (nano-C-60, nC(60)) is extremely toxic, but the mechanisms of its cytotoxicity are not completely understood. By combining experimental analysis and mathematical modelling, we investigate the requirements for the reactive oxygen species (ROS)-mediated cytotoxicity of different nC(60) suspensions, prepared by solvent exchange method in tetrahydrofuran (THF/nC(60)) and ethanol (EtOH/nC(60)), or by extended mixing in water (aqu/nC(60)). With regard to their capacity to generate ROS and cause mitochondrial depolarization followed by necrotic cell death, the nC(60) suspensions are ranked in the following order: THF/nC(60) GT EtOH/nC(60) GT aqu/nC(60). Mathematical modelling of singlet oxygen (O-1(2)) generation indicates that the O-1(2)-quenching power (THF/nC(60) LT EtOH/nC(60) LT aqU/nC(60)) of the solvent intercalated in the fullerene crystals determines their ability to produce ROS and cause cell damage. These data could. have important implications for toxicology and biomedical application of colloidal fullerenes. (C) 2007 Elsevier Ltd. All rights reserved.",
journal = "Biomaterials",
title = "The mechanism of cell-damaging reactive oxygen generation by colloidal fullerenes",
volume = "28",
number = "36",
pages = "5437-5448",
doi = "10.1016/j.biomaterials.2007.09.002"
}
Marković, Z. M., Todorović-Marković, B., Kleut, D., Nikolić, N. S., Vranješ-Đurić, S., Misirkić, M., Vucicevic, L., Janjetović, K. D., Isaković, A. J., Harhaji, L. M., Babić-Stojić Branka S., Dramićanin, M.,& Trajković, V. S. (2007). The mechanism of cell-damaging reactive oxygen generation by colloidal fullerenes.
Biomaterials, 28(36), 5437-5448.
https://doi.org/10.1016/j.biomaterials.2007.09.002
Marković ZM, Todorović-Marković B, Kleut D, Nikolić NS, Vranješ-Đurić S, Misirkić M, Vucicevic L, Janjetović KD, Isaković AJ, Harhaji LM, Babić-Stojić Branka S., Dramićanin M, Trajković VS. The mechanism of cell-damaging reactive oxygen generation by colloidal fullerenes. Biomaterials. 2007;28(36):5437-5448
Marković Zoran M., Todorović-Marković Biljana, Kleut Duška, Nikolić Nadežda S., Vranješ-Đurić Sanja, Misirkić Maja, Vucicevic Ljubica, Janjetović Kristina D., Isaković Aleksandra J., Harhaji Ljubica M., Babić-Stojić Branka S., Dramićanin Miroslav, Trajković Vladimir S., "The mechanism of cell-damaging reactive oxygen generation by colloidal fullerenes" Biomaterials, 28, no. 36 (2007):5437-5448,
https://doi.org/10.1016/j.biomaterials.2007.09.002 .
99
94
103

Inactivation of nanocrystalline C-60 cytotoxicity by gamma-irradiation

Isaković, Aleksandra J.; Marković, Zoran M.; Nikolić, Nadežda S.; Todorović-Marković, Biljana; Vranješ-Đurić, Sanja; Harhaji, Ljubica M.; Raičević, Nevena; Romčević, Nebojša Ž.; Vasiljević-Radović, Dana; Dramićanin, Miroslav; Trajković, Vladimir S.

(2006)

TY  - JOUR
AU  - Isaković, Aleksandra J.
AU  - Marković, Zoran M.
AU  - Nikolić, Nadežda S.
AU  - Todorović-Marković, Biljana
AU  - Vranješ-Đurić, Sanja
AU  - Harhaji, Ljubica M.
AU  - Raičević, Nevena
AU  - Romčević, Nebojša Ž.
AU  - Vasiljević-Radović, Dana
AU  - Dramićanin, Miroslav
AU  - Trajković, Vladimir S.
PY  - 2006
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3076
AB  - We investigated the effect of gamma-irradiation on the cytotoxicity of pure C-60 solubilized in water by using tetrahydrofuran (THF/n-C-60 or THF/n-C-60). In contrast to THF/n-C-60, its gamma-irradiated counterpart failed to generate oxygen radicals and cause extracellular signal-regulated kinase (ERK)-dependent necrotic cell death in various types of mammalian cells. Moreover, gamma-irradiated THF/n-C-60 protected cells from the oxidative stress induced by native THF/n-C-60 or hydrogen peroxide. The observed biological effects were associated with of THF and subsequent derivatization of the n-C-60 surface. These results for the first time demonstrate gamma-irradiation-mediated changes in the physico-chemical properties of THF-prepared nanocrystalline C-60, resulting in a complete loss of its cytotoxic effect and its conversion to a cytoprotective agent. (c) 2006 Elsevier Ltd. All rights reserved.
T2  - Biomaterials
T1  - Inactivation of nanocrystalline C-60 cytotoxicity by gamma-irradiation
VL  - 27
IS  - 29
SP  - 5049
EP  - 5058
DO  - 10.1016/j.biomaterials.2006.05.047
ER  - 
@article{
author = "Isaković, Aleksandra J. and Marković, Zoran M. and Nikolić, Nadežda S. and Todorović-Marković, Biljana and Vranješ-Đurić, Sanja and Harhaji, Ljubica M. and Raičević, Nevena and Romčević, Nebojša Ž. and Vasiljević-Radović, Dana and Dramićanin, Miroslav and Trajković, Vladimir S.",
year = "2006",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/3076",
abstract = "We investigated the effect of gamma-irradiation on the cytotoxicity of pure C-60 solubilized in water by using tetrahydrofuran (THF/n-C-60 or THF/n-C-60). In contrast to THF/n-C-60, its gamma-irradiated counterpart failed to generate oxygen radicals and cause extracellular signal-regulated kinase (ERK)-dependent necrotic cell death in various types of mammalian cells. Moreover, gamma-irradiated THF/n-C-60 protected cells from the oxidative stress induced by native THF/n-C-60 or hydrogen peroxide. The observed biological effects were associated with of THF and subsequent derivatization of the n-C-60 surface. These results for the first time demonstrate gamma-irradiation-mediated changes in the physico-chemical properties of THF-prepared nanocrystalline C-60, resulting in a complete loss of its cytotoxic effect and its conversion to a cytoprotective agent. (c) 2006 Elsevier Ltd. All rights reserved.",
journal = "Biomaterials",
title = "Inactivation of nanocrystalline C-60 cytotoxicity by gamma-irradiation",
volume = "27",
number = "29",
pages = "5049-5058",
doi = "10.1016/j.biomaterials.2006.05.047"
}
Isaković, A. J., Marković, Z. M., Nikolić, N. S., Todorović-Marković, B., Vranješ-Đurić, S., Harhaji, L. M., Raičević, N., Romčević, N. Ž., Vasiljević-Radović, D., Dramićanin, M.,& Trajković, V. S. (2006). Inactivation of nanocrystalline C-60 cytotoxicity by gamma-irradiation.
Biomaterials, 27(29), 5049-5058.
https://doi.org/10.1016/j.biomaterials.2006.05.047
Isaković AJ, Marković ZM, Nikolić NS, Todorović-Marković B, Vranješ-Đurić S, Harhaji LM, Raičević N, Romčević NŽ, Vasiljević-Radović D, Dramićanin M, Trajković VS. Inactivation of nanocrystalline C-60 cytotoxicity by gamma-irradiation. Biomaterials. 2006;27(29):5049-5058
Isaković Aleksandra J., Marković Zoran M., Nikolić Nadežda S., Todorović-Marković Biljana, Vranješ-Đurić Sanja, Harhaji Ljubica M., Raičević Nevena, Romčević Nebojša Ž., Vasiljević-Radović Dana, Dramićanin Miroslav, Trajković Vladimir S., "Inactivation of nanocrystalline C-60 cytotoxicity by gamma-irradiation" Biomaterials, 27, no. 29 (2006):5049-5058,
https://doi.org/10.1016/j.biomaterials.2006.05.047 .
61
56
63

Distinct cytotoxic mechanisms of pristine versus hydroxylated fullerene

Isaković, Aleksandra J.; Marković, Zoran M.; Todorović-Marković, Biljana; Nikolić, Nadežda S.; Vranješ-Đurić, Sanja; Mirković, Marija D.; Dramićanin, Miroslav; Harhaji, Ljubica M.; Raičević, Nevena; Nikolić, Zoran M.; Trajković, Vladimir S.

(2006)

TY  - JOUR
AU  - Isaković, Aleksandra J.
AU  - Marković, Zoran M.
AU  - Todorović-Marković, Biljana
AU  - Nikolić, Nadežda S.
AU  - Vranješ-Đurić, Sanja
AU  - Mirković, Marija D.
AU  - Dramićanin, Miroslav
AU  - Harhaji, Ljubica M.
AU  - Raičević, Nevena
AU  - Nikolić, Zoran M.
AU  - Trajković, Vladimir S.
PY  - 2006
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3002
AB  - The mechanisms underlying the cytotoxic action of pure fullerene suspension (nano-C-60) and water-soluble polyhydroxylated fullerene [C-60(OH)(n)] were investigated. Crystal violet assay for cell viability demonstrated that nano-C-60 was at least three orders of magnitude more toxic than C-60(OH)(n) to mouse L929 fibrosarcoma, rat C6 glioma, and U251 human glioma cell lines. Flow cytometry analysis of cells stained with propidium iodide (PI), PI/annexin V-fluorescein isothiocyanate, or the redox-sensitive dye dihydrorhodamine revealed that nano-C-60 caused rapid (observable after few hours), reactive oxygen species (ROS)-associated necrosis characterized by cell membrane damage without DNA fragmentation. In contrast, C-60(OH)(n) caused delayed, ROS-independent cell death with characteristics of apoptosis, including DNA fragmentation and loss of cell membrane asymmetry in the absence of increased permeability. Accordingly, the antioxidant N-acetylcysteine protected the cell lines from nano-C-60 toxicity, but not C-60(OH)(n) toxicity, while the pan-caspase inhibitor z-VAD-fmk blocked C-60(OH)(n)-induced apoptosis, but not nano-C-60-mediated necrosis. Finally, C-60(OH)(n) antagonized, while nano-C-60 synergized with, the cytotoxic action of oxidative stress-inducing agents hydrogen peroxide and peroxynitrite donor 3-morpholinosydnonimine. Therefore, unlike polyhydroxylated C-60 that exerts mainly antioxidant/cytoprotective and only mild ROS-independent pro-apoptotic activity, pure crystalline C-60 seems to be endowed with strong pro-oxidant capacity responsible for the rapid necrotic cell death.
T2  - Toxicological Sciences
T1  - Distinct cytotoxic mechanisms of pristine versus hydroxylated fullerene
VL  - 91
IS  - 1
SP  - 173
EP  - 183
DO  - 10.1093/toxsci/kfj127
ER  - 
@article{
author = "Isaković, Aleksandra J. and Marković, Zoran M. and Todorović-Marković, Biljana and Nikolić, Nadežda S. and Vranješ-Đurić, Sanja and Mirković, Marija D. and Dramićanin, Miroslav and Harhaji, Ljubica M. and Raičević, Nevena and Nikolić, Zoran M. and Trajković, Vladimir S.",
year = "2006",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/3002",
abstract = "The mechanisms underlying the cytotoxic action of pure fullerene suspension (nano-C-60) and water-soluble polyhydroxylated fullerene [C-60(OH)(n)] were investigated. Crystal violet assay for cell viability demonstrated that nano-C-60 was at least three orders of magnitude more toxic than C-60(OH)(n) to mouse L929 fibrosarcoma, rat C6 glioma, and U251 human glioma cell lines. Flow cytometry analysis of cells stained with propidium iodide (PI), PI/annexin V-fluorescein isothiocyanate, or the redox-sensitive dye dihydrorhodamine revealed that nano-C-60 caused rapid (observable after few hours), reactive oxygen species (ROS)-associated necrosis characterized by cell membrane damage without DNA fragmentation. In contrast, C-60(OH)(n) caused delayed, ROS-independent cell death with characteristics of apoptosis, including DNA fragmentation and loss of cell membrane asymmetry in the absence of increased permeability. Accordingly, the antioxidant N-acetylcysteine protected the cell lines from nano-C-60 toxicity, but not C-60(OH)(n) toxicity, while the pan-caspase inhibitor z-VAD-fmk blocked C-60(OH)(n)-induced apoptosis, but not nano-C-60-mediated necrosis. Finally, C-60(OH)(n) antagonized, while nano-C-60 synergized with, the cytotoxic action of oxidative stress-inducing agents hydrogen peroxide and peroxynitrite donor 3-morpholinosydnonimine. Therefore, unlike polyhydroxylated C-60 that exerts mainly antioxidant/cytoprotective and only mild ROS-independent pro-apoptotic activity, pure crystalline C-60 seems to be endowed with strong pro-oxidant capacity responsible for the rapid necrotic cell death.",
journal = "Toxicological Sciences",
title = "Distinct cytotoxic mechanisms of pristine versus hydroxylated fullerene",
volume = "91",
number = "1",
pages = "173-183",
doi = "10.1093/toxsci/kfj127"
}
Isaković, A. J., Marković, Z. M., Todorović-Marković, B., Nikolić, N. S., Vranješ-Đurić, S., Mirković, M. D., Dramićanin, M., Harhaji, L. M., Raičević, N., Nikolić, Z. M.,& Trajković, V. S. (2006). Distinct cytotoxic mechanisms of pristine versus hydroxylated fullerene.
Toxicological Sciences, 91(1), 173-183.
https://doi.org/10.1093/toxsci/kfj127
Isaković AJ, Marković ZM, Todorović-Marković B, Nikolić NS, Vranješ-Đurić S, Mirković MD, Dramićanin M, Harhaji LM, Raičević N, Nikolić ZM, Trajković VS. Distinct cytotoxic mechanisms of pristine versus hydroxylated fullerene. Toxicological Sciences. 2006;91(1):173-183
Isaković Aleksandra J., Marković Zoran M., Todorović-Marković Biljana, Nikolić Nadežda S., Vranješ-Đurić Sanja, Mirković Marija D., Dramićanin Miroslav, Harhaji Ljubica M., Raičević Nevena, Nikolić Zoran M., Trajković Vladimir S., "Distinct cytotoxic mechanisms of pristine versus hydroxylated fullerene" Toxicological Sciences, 91, no. 1 (2006):173-183,
https://doi.org/10.1093/toxsci/kfj127 .
1
233
223
248

Chiral ligand-exchange chromatography as the screening method for proposed modifications in exametazime synthesis to enhance diastereoselectivity

Nikolić, Nadežda S.; Veselinović, Dragan S.; Vladimirov, Sote; Karljiković-Rajić, Katarina; Lingeman, H

(2004)

TY  - JOUR
AU  - Nikolić, Nadežda S.
AU  - Veselinović, Dragan S.
AU  - Vladimirov, Sote
AU  - Karljiković-Rajić, Katarina
AU  - Lingeman, H
PY  - 2004
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/2714
AB  - Tc-99m(V)-d,l-HM-PAO complex is well-known radiopharmaceutical for regional cerebral blood flow imaging. The proposed modifications in exametazime, hexamethylpropyleneamine oxime (HM-PAO) (4,8-diaza-3,6,6,9-tetramethylundecane-2,10-dione bisoxime) synthesis, for reduction of intermediary reactant diiminebisoxime (DI) (4,8-diaza-3,6,6,9-tetramethylundecane-3,8-diene-2,10-dione bisoxime) concerned two reductants (NaBH4 and KBH4), two solvents (ethanol and 2-propanol), and three mole ratios of reactant/reductants (1:1, 1:1.5, and 1:2). The simultaneous analysis of diastereo-enantiomeric HM-PAO content, as well as the content of starting DI, in different reduction mixtures were pet-formed using chiral ligand-exchange chromatography (CLEC). The separation of the samples of investigated reduction mixtures, obtained in the second step of HM-PAO synthesis, has been accomplished by using an achiral sorbent (RP- 18) and a chiral mobile phase (CMP) containing copper(II) complex with N,N-ditnethyl-L-phenylalanine (L-DM-PhA) as initial complex for CLEC. With 12 different reduction conditions, the obtained ratios of diastereoisomers d,l-HM-PAO: mesa-HM-PAO varied from 69.2:30.8 to 15.9:84.1, in comparison to the reduction in routine synthesis of HM-PAO which gives an equal mixture of diastereoisomers. The ternary mixed complexes formation recorded spectrophotometrically on addition of HM-PAO or DI to the mobile phase with binary complex Cu(L-DM-PhA)(2), due to the evidence of bathochromic shift of 46 nm for lambda(max) with significant difference in absorptivity contributes to separation mechanism. (C) 2003 Elsevier B.V. All rights reserved.
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Chiral ligand-exchange chromatography as the screening method for proposed modifications in exametazime synthesis to enhance diastereoselectivity
VL  - 34
IS  - 2
SP  - 285
EP  - 293
DO  - 10.1016/S0731-7085(03)00551-X
ER  - 
@article{
author = "Nikolić, Nadežda S. and Veselinović, Dragan S. and Vladimirov, Sote and Karljiković-Rajić, Katarina and Lingeman, H",
year = "2004",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/2714",
abstract = "Tc-99m(V)-d,l-HM-PAO complex is well-known radiopharmaceutical for regional cerebral blood flow imaging. The proposed modifications in exametazime, hexamethylpropyleneamine oxime (HM-PAO) (4,8-diaza-3,6,6,9-tetramethylundecane-2,10-dione bisoxime) synthesis, for reduction of intermediary reactant diiminebisoxime (DI) (4,8-diaza-3,6,6,9-tetramethylundecane-3,8-diene-2,10-dione bisoxime) concerned two reductants (NaBH4 and KBH4), two solvents (ethanol and 2-propanol), and three mole ratios of reactant/reductants (1:1, 1:1.5, and 1:2). The simultaneous analysis of diastereo-enantiomeric HM-PAO content, as well as the content of starting DI, in different reduction mixtures were pet-formed using chiral ligand-exchange chromatography (CLEC). The separation of the samples of investigated reduction mixtures, obtained in the second step of HM-PAO synthesis, has been accomplished by using an achiral sorbent (RP- 18) and a chiral mobile phase (CMP) containing copper(II) complex with N,N-ditnethyl-L-phenylalanine (L-DM-PhA) as initial complex for CLEC. With 12 different reduction conditions, the obtained ratios of diastereoisomers d,l-HM-PAO: mesa-HM-PAO varied from 69.2:30.8 to 15.9:84.1, in comparison to the reduction in routine synthesis of HM-PAO which gives an equal mixture of diastereoisomers. The ternary mixed complexes formation recorded spectrophotometrically on addition of HM-PAO or DI to the mobile phase with binary complex Cu(L-DM-PhA)(2), due to the evidence of bathochromic shift of 46 nm for lambda(max) with significant difference in absorptivity contributes to separation mechanism. (C) 2003 Elsevier B.V. All rights reserved.",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Chiral ligand-exchange chromatography as the screening method for proposed modifications in exametazime synthesis to enhance diastereoselectivity",
volume = "34",
number = "2",
pages = "285-293",
doi = "10.1016/S0731-7085(03)00551-X"
}
Nikolić, N. S., Veselinović, D. S., Vladimirov, S., Karljiković-Rajić, K.,& Lingeman, H. (2004). Chiral ligand-exchange chromatography as the screening method for proposed modifications in exametazime synthesis to enhance diastereoselectivity.
Journal of Pharmaceutical and Biomedical Analysis, 34(2), 285-293.
https://doi.org/10.1016/S0731-7085(03)00551-X
Nikolić NS, Veselinović DS, Vladimirov S, Karljiković-Rajić K, Lingeman H. Chiral ligand-exchange chromatography as the screening method for proposed modifications in exametazime synthesis to enhance diastereoselectivity. Journal of Pharmaceutical and Biomedical Analysis. 2004;34(2):285-293
Nikolić Nadežda S., Veselinović Dragan S., Vladimirov Sote, Karljiković-Rajić Katarina, Lingeman H, "Chiral ligand-exchange chromatography as the screening method for proposed modifications in exametazime synthesis to enhance diastereoselectivity" Journal of Pharmaceutical and Biomedical Analysis, 34, no. 2 (2004):285-293,
https://doi.org/10.1016/S0731-7085(03)00551-X .
2
2
2

Chiral ligand-exchange chromatography for diastereo-enantio separation of exametazime

Nikolić, Nadežda S.; Veselinović, Dragan S.; Vučina, Jurij L.; Lingeman, H; Karljiković-Rajić, Katarina

(2003)

TY  - JOUR
AU  - Nikolić, Nadežda S.
AU  - Veselinović, Dragan S.
AU  - Vučina, Jurij L.
AU  - Lingeman, H
AU  - Karljiković-Rajić, Katarina
PY  - 2003
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/2661
AB  - The diastereo-enantio separation of isomeric mixtures of exametazime (HM-PAO) by liquid chromatography is described using an achiral sorbent (RP-18). A chiral eluent with the initial complex of Cu(II) and the optically active selector N,N-dimethyl-l-phenylalanine (l-DM-PhA), based on the ligand-exchange principle, has been applied. The separation is based on the presence of the immobilized binary complex Cu(l-DM-PhA)(2) and formation of mixed ternary complex. The optimal mole ratio of Cu(II):l-DM-PhA is 1:4, the pH should be between 4.1 and 4.2 and up to 0.8 mM of triethylamine is added for column presaturation with the initial complex. The elution order has been defined using isolated l-HM-PAO via l-HM-PAO L(+)tartrate and tneso-HM-PAO obtained by repeated recrystallization from the isomeric mixture of HM-PAO. Complete resolution between all isomers (R-S from 2.14 to 3.91) and partial resolution for meso(EE)/l-HM-PAO (R-S = 0.83) has been obtained. This means that the proposed chiral ligand-exchange chromatography (CLEC) can be used for determination of the isomeric purity of HM-PAO. This as an alternative method for resolution measurements with chiral columns. (C) 2003 Elsevier B.V. All rights reserved.
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Chiral ligand-exchange chromatography for diastereo-enantio separation of exametazime
VL  - 32
IS  - 6
SP  - 1159
EP  - 1166
DO  - 10.1016/S0731-7085(03)00230-9
ER  - 
@article{
author = "Nikolić, Nadežda S. and Veselinović, Dragan S. and Vučina, Jurij L. and Lingeman, H and Karljiković-Rajić, Katarina",
year = "2003",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/2661",
abstract = "The diastereo-enantio separation of isomeric mixtures of exametazime (HM-PAO) by liquid chromatography is described using an achiral sorbent (RP-18). A chiral eluent with the initial complex of Cu(II) and the optically active selector N,N-dimethyl-l-phenylalanine (l-DM-PhA), based on the ligand-exchange principle, has been applied. The separation is based on the presence of the immobilized binary complex Cu(l-DM-PhA)(2) and formation of mixed ternary complex. The optimal mole ratio of Cu(II):l-DM-PhA is 1:4, the pH should be between 4.1 and 4.2 and up to 0.8 mM of triethylamine is added for column presaturation with the initial complex. The elution order has been defined using isolated l-HM-PAO via l-HM-PAO L(+)tartrate and tneso-HM-PAO obtained by repeated recrystallization from the isomeric mixture of HM-PAO. Complete resolution between all isomers (R-S from 2.14 to 3.91) and partial resolution for meso(EE)/l-HM-PAO (R-S = 0.83) has been obtained. This means that the proposed chiral ligand-exchange chromatography (CLEC) can be used for determination of the isomeric purity of HM-PAO. This as an alternative method for resolution measurements with chiral columns. (C) 2003 Elsevier B.V. All rights reserved.",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Chiral ligand-exchange chromatography for diastereo-enantio separation of exametazime",
volume = "32",
number = "6",
pages = "1159-1166",
doi = "10.1016/S0731-7085(03)00230-9"
}
Nikolić, N. S., Veselinović, D. S., Vučina, J. L., Lingeman, H.,& Karljiković-Rajić, K. (2003). Chiral ligand-exchange chromatography for diastereo-enantio separation of exametazime.
Journal of Pharmaceutical and Biomedical Analysis, 32(6), 1159-1166.
https://doi.org/10.1016/S0731-7085(03)00230-9
Nikolić NS, Veselinović DS, Vučina JL, Lingeman H, Karljiković-Rajić K. Chiral ligand-exchange chromatography for diastereo-enantio separation of exametazime. Journal of Pharmaceutical and Biomedical Analysis. 2003;32(6):1159-1166
Nikolić Nadežda S., Veselinović Dragan S., Vučina Jurij L., Lingeman H, Karljiković-Rajić Katarina, "Chiral ligand-exchange chromatography for diastereo-enantio separation of exametazime" Journal of Pharmaceutical and Biomedical Analysis, 32, no. 6 (2003):1159-1166,
https://doi.org/10.1016/S0731-7085(03)00230-9 .
7
9
7