TY  - JOUR
AU  - Stojković, Ljiljana
AU  - Zec, Manja
AU  - Živković, Maja
AU  - Bundalo, Maja
AU  - Bošković, Maja
AU  - Glibetić, Marija
AU  - Stanković, Aleksandra
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9865
AB  - Cardiovascular disease (CVD) is associated with alterations in DNA methylation and polyunsaturated fatty acid (PUFA) profile, both modulated by dietary polyphenols. The present parallel, placebo-controlled study (part of the original clinical study registered as NCT02800967 at www.clinicaltrials.gov) aimed to determine the impact of 4-week daily consumption of polyphenol-rich Aronia melanocarpa juice (AMJ) treatment on Long Interspersed Nucleotide Element-1 (LINE-1) methylation in peripheral blood leukocytes and on plasma PUFAs, in subjects (n = 54, age range of 40.2 ± 6.7 years) at moderate CVD risk, including an increased body mass index, central obesity, high normal blood pressure, and/or dyslipidemia. The goal was also to examine whether factors known to affect DNA methylation (folate intake levels, MTHFR C677T gene variant, anthropometric and metabolic parameters) modulated the LINE-1 methylation levels upon the consumption of polyphenol-rich aronia juice. Experimental analysis of LINE-1 methylation was done by MethyLight method. MTHFR C677T genotypes were determined by the polymerase chain reaction–restriction fragment length polymorphism method, and folate intake was assessed by processing the data from the food frequency questionnaire. PUFAs were measured by gas–liquid chromatography, and serum lipid profile was determined by using Roche Diagnostics kits. The statistical analyses were performed using Statistica software package. In the comparison after vs. before the treatment period, in dyslipidemic women (n = 22), we observed significant decreases in LINE-1 methylation levels (97.54 ± 1.50 vs. 98.39 ± 0.86%, respectively; P = 0.01) and arachidonic acid/eicosapentaenoic acid ratio [29.17 ± 15.21 vs. 38.42 (25.96–89.58), respectively; P = 0.02]. The change (after vs. before treatment) in LINE-1 methylation directly correlated with the presence of MTHFR 677T allele, average daily folate intake, and the change in serum low-density lipoprotein cholesterol but inversely correlated with the change in serum triacylglycerols (R = 0.72, R2 = 0.52, adjusted R2 = 0.36, P = 0.03). The current results imply potential cardioprotective effects of habitual polyphenol-rich aronia juice consumption achieved through the modifications of DNA methylation pattern and PUFAs in subjects at CVD risk, which should be further confirmed. Hence, the precision nutrition-driven modulations of both DNA methylation and PUFA profile may become targets for new approaches in the prevention of CVD.
T2  - Frontiers in Nutrition
T1  - Polyphenol-Rich Aronia melanocarpa Juice Consumption Affects LINE-1 DNA Methylation in Peripheral Blood Leukocytes in Dyslipidemic Women
VL  - 8
DO  - 10.3389/fnut.2021.689055
ER  - 

@article{
author = "Stojković, Ljiljana and Zec, Manja and Živković, Maja and Bundalo, Maja and Bošković, Maja and Glibetić, Marija and Stanković, Aleksandra",
year = "2021",
abstract = "Cardiovascular disease (CVD) is associated with alterations in DNA methylation and polyunsaturated fatty acid (PUFA) profile, both modulated by dietary polyphenols. The present parallel, placebo-controlled study (part of the original clinical study registered as NCT02800967 at www.clinicaltrials.gov) aimed to determine the impact of 4-week daily consumption of polyphenol-rich Aronia melanocarpa juice (AMJ) treatment on Long Interspersed Nucleotide Element-1 (LINE-1) methylation in peripheral blood leukocytes and on plasma PUFAs, in subjects (n = 54, age range of 40.2 ± 6.7 years) at moderate CVD risk, including an increased body mass index, central obesity, high normal blood pressure, and/or dyslipidemia. The goal was also to examine whether factors known to affect DNA methylation (folate intake levels, MTHFR C677T gene variant, anthropometric and metabolic parameters) modulated the LINE-1 methylation levels upon the consumption of polyphenol-rich aronia juice. Experimental analysis of LINE-1 methylation was done by MethyLight method. MTHFR C677T genotypes were determined by the polymerase chain reaction–restriction fragment length polymorphism method, and folate intake was assessed by processing the data from the food frequency questionnaire. PUFAs were measured by gas–liquid chromatography, and serum lipid profile was determined by using Roche Diagnostics kits. The statistical analyses were performed using Statistica software package. In the comparison after vs. before the treatment period, in dyslipidemic women (n = 22), we observed significant decreases in LINE-1 methylation levels (97.54 ± 1.50 vs. 98.39 ± 0.86%, respectively; P = 0.01) and arachidonic acid/eicosapentaenoic acid ratio [29.17 ± 15.21 vs. 38.42 (25.96–89.58), respectively; P = 0.02]. The change (after vs. before treatment) in LINE-1 methylation directly correlated with the presence of MTHFR 677T allele, average daily folate intake, and the change in serum low-density lipoprotein cholesterol but inversely correlated with the change in serum triacylglycerols (R = 0.72, R2 = 0.52, adjusted R2 = 0.36, P = 0.03). The current results imply potential cardioprotective effects of habitual polyphenol-rich aronia juice consumption achieved through the modifications of DNA methylation pattern and PUFAs in subjects at CVD risk, which should be further confirmed. Hence, the precision nutrition-driven modulations of both DNA methylation and PUFA profile may become targets for new approaches in the prevention of CVD.",
journal = "Frontiers in Nutrition",
title = "Polyphenol-Rich Aronia melanocarpa Juice Consumption Affects LINE-1 DNA Methylation in Peripheral Blood Leukocytes in Dyslipidemic Women",
volume = "8",
doi = "10.3389/fnut.2021.689055"
}

Stojković, L., Zec, M., Živković, M., Bundalo, M., Bošković, M., Glibetić, M.,& Stanković, A.. (2021). Polyphenol-Rich Aronia melanocarpa Juice Consumption Affects LINE-1 DNA Methylation in Peripheral Blood Leukocytes in Dyslipidemic Women. in Frontiers in Nutrition, 8.
https://doi.org/10.3389/fnut.2021.689055

Stojković L, Zec M, Živković M, Bundalo M, Bošković M, Glibetić M, Stanković A. Polyphenol-Rich Aronia melanocarpa Juice Consumption Affects LINE-1 DNA Methylation in Peripheral Blood Leukocytes in Dyslipidemic Women. in Frontiers in Nutrition. 2021;8.
doi:10.3389/fnut.2021.689055 .

Stojković, Ljiljana, Zec, Manja, Živković, Maja, Bundalo, Maja, Bošković, Maja, Glibetić, Marija, Stanković, Aleksandra, "Polyphenol-Rich Aronia melanocarpa Juice Consumption Affects LINE-1 DNA Methylation in Peripheral Blood Leukocytes in Dyslipidemic Women" in Frontiers in Nutrition, 8 (2021),
https://doi.org/10.3389/fnut.2021.689055 . .

TY  - JOUR
AU  - Stojković, Ljiljana S.
AU  - Jovanović, Ivan G.
AU  - Živković, Maja
AU  - Zec, Manja
AU  - Đurić, Tamara
AU  - Životić, Ivan
AU  - Kuveljić, Jovana
AU  - Kolaković, Ana
AU  - Kolić, Ivana
AU  - Đorđević, Ana
AU  - Glibetić, Marija
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8683
AB  - Foods and food products that contain polyphenols are proposed to modulate risk of cardiovascular disease. The aim of this three-arm, crossover, randomized, double-blind, placebo-controlled intervention study was to examine the impact of Aronia melanocarpa juice (AMJ), high-polyphenol (AMJ treatment, 1.17 g/100 mL polyphenols) and low-polyphenol (dAMJ treatment, 0.29 g/100 mL polyphenols) dose, on the transcriptome in peripheral blood mononuclear cells (PBMC) of 19 subjects at cardiovascular risk. Transcriptome data were obtained by microarray. Bioinformatic functional annotation analysis was performed on both the whole transcriptome datasets and the differentially expressed genes (DEGs). Expression of selected DEGs was validated by RT-qPCR. Administration of AMJ and dAMJ treatments during the two consecutive four-week treatment periods had additive effects on PBMC transcriptome profiles, with the most pronounced and specific effect noticed for AMJ in the last treatment period (TP3) of the trial. Between the high-dose and low-dose treatments in TP3, there was a multitude of overlapping DEGs and DEG-enriched biological processes and pathways, which primarily included immunomodulation and regulation of cell proliferation/death. Increased expression of TNF, IL1B, IL8, RGS1, OSM, and DUSP2 in TP3 was confirmed by RT-qPCR. The results suggest the immunomodulatory effects of prolonged habitual consumption of polyphenol-rich aronia juice in individuals at cardiovascular risk.
T2  - Nutrients
T1  - The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk
VL  - 12
IS  - 5
SP  - 1484
DO  - 10.3390/nu12051484
ER  - 

@article{
author = "Stojković, Ljiljana S. and Jovanović, Ivan G. and Živković, Maja and Zec, Manja and Đurić, Tamara and Životić, Ivan and Kuveljić, Jovana and Kolaković, Ana and Kolić, Ivana and Đorđević, Ana and Glibetić, Marija and Alavantić, Dragan and Stanković, Aleksandra",
year = "2020",
abstract = "Foods and food products that contain polyphenols are proposed to modulate risk of cardiovascular disease. The aim of this three-arm, crossover, randomized, double-blind, placebo-controlled intervention study was to examine the impact of Aronia melanocarpa juice (AMJ), high-polyphenol (AMJ treatment, 1.17 g/100 mL polyphenols) and low-polyphenol (dAMJ treatment, 0.29 g/100 mL polyphenols) dose, on the transcriptome in peripheral blood mononuclear cells (PBMC) of 19 subjects at cardiovascular risk. Transcriptome data were obtained by microarray. Bioinformatic functional annotation analysis was performed on both the whole transcriptome datasets and the differentially expressed genes (DEGs). Expression of selected DEGs was validated by RT-qPCR. Administration of AMJ and dAMJ treatments during the two consecutive four-week treatment periods had additive effects on PBMC transcriptome profiles, with the most pronounced and specific effect noticed for AMJ in the last treatment period (TP3) of the trial. Between the high-dose and low-dose treatments in TP3, there was a multitude of overlapping DEGs and DEG-enriched biological processes and pathways, which primarily included immunomodulation and regulation of cell proliferation/death. Increased expression of TNF, IL1B, IL8, RGS1, OSM, and DUSP2 in TP3 was confirmed by RT-qPCR. The results suggest the immunomodulatory effects of prolonged habitual consumption of polyphenol-rich aronia juice in individuals at cardiovascular risk.",
journal = "Nutrients",
title = "The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk",
volume = "12",
number = "5",
pages = "1484",
doi = "10.3390/nu12051484"
}

Stojković, L. S., Jovanović, I. G., Živković, M., Zec, M., Đurić, T., Životić, I., Kuveljić, J., Kolaković, A., Kolić, I., Đorđević, A., Glibetić, M., Alavantić, D.,& Stanković, A.. (2020). The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk. in Nutrients, 12(5), 1484.
https://doi.org/10.3390/nu12051484

Stojković LS, Jovanović IG, Živković M, Zec M, Đurić T, Životić I, Kuveljić J, Kolaković A, Kolić I, Đorđević A, Glibetić M, Alavantić D, Stanković A. The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk. in Nutrients. 2020;12(5):1484.
doi:10.3390/nu12051484 .

Stojković, Ljiljana S., Jovanović, Ivan G., Živković, Maja, Zec, Manja, Đurić, Tamara, Životić, Ivan, Kuveljić, Jovana, Kolaković, Ana, Kolić, Ivana, Đorđević, Ana, Glibetić, Marija, Alavantić, Dragan, Stanković, Aleksandra, "The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk" in Nutrients, 12, no. 5 (2020):1484,
https://doi.org/10.3390/nu12051484 . .

TY  - JOUR
AU  - Zec, Manja
AU  - Stojković, Ljiljana S.
AU  - Zeković, Milica
AU  - Pokimica, Biljana
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Glibetić, Marija
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8883
AB  - Polymorphisms in FADS genes are associated with plasma long-chain polyunsaturated fatty acids (LC-PUFA) and modulate omega-6/omega-3 balance. We hypothesized that the FADS2 gene variants will be associated with lower product-to-precursor ratio in the fatty acid metabolic pathways. Thus, we explored FADS2 rs174593, rs174616, and rs174576 effects on plasma phospholipid fatty acid profile, markers of desaturase activities, and risk factors in a sample of apparently healthy Serbian adults. Food and nutrient intake data were compiled through 24 h recalls. Plasma phospholipid fatty acid content was assessed by gas-chromatography. Estimated desaturase activities were calculated as conversion rates towards LC-PUFA in omega-6 pathway. During the selection of FADS2 polymorphisms, we accounted for their positional and functional aspect. Genotyping was performed by Real-Time PCR. Multivariable-adjusted general linear and hierarchical regression models were applied. Study subjects (mean age = 40 ± 7 years, 70% who were overweight) had a median dietary omega-6/omega-3 ratio of 16.29. Alternative allele frequencies were 33%, 36%, and 51% for rs174593, rs174576, and rs174616, respectively. Addition of FADS2 alternative alleles was associated with lower plasma arachidonic acid (AA, C20:4 n-6, P < .001) and estimated desaturase-5 activity (P < .001), irrespective of gender, age, daily polyunsaturated/saturated fatty acid intake, and obesity. The rs174576 association with AA withstood multiple testing and additional adjustments for other variants (multivariable-adjusted β = −1.14 [95% CI: −2.25, −0.43]). None of the variants was associated with dietary intake, serum lipids, or obesity. We observed inverse associations between FADS2 variants and plasma AA but not omega-3 fatty acids in Serbian subjects, with rs174576 exhibiting the strongest relation.
T2  - Nutrition Research
T1  - FADS2 polymorphisms are associated with plasma arachidonic acid and estimated desaturase-5 activity in a cross-sectional study
VL  - 83
SP  - 49
EP  - 62
DO  - 10.1016/j.nutres.2020.08.010
ER  - 

@article{
author = "Zec, Manja and Stojković, Ljiljana S. and Zeković, Milica and Pokimica, Biljana and Živković, Maja and Stanković, Aleksandra and Glibetić, Marija",
year = "2020",
abstract = "Polymorphisms in FADS genes are associated with plasma long-chain polyunsaturated fatty acids (LC-PUFA) and modulate omega-6/omega-3 balance. We hypothesized that the FADS2 gene variants will be associated with lower product-to-precursor ratio in the fatty acid metabolic pathways. Thus, we explored FADS2 rs174593, rs174616, and rs174576 effects on plasma phospholipid fatty acid profile, markers of desaturase activities, and risk factors in a sample of apparently healthy Serbian adults. Food and nutrient intake data were compiled through 24 h recalls. Plasma phospholipid fatty acid content was assessed by gas-chromatography. Estimated desaturase activities were calculated as conversion rates towards LC-PUFA in omega-6 pathway. During the selection of FADS2 polymorphisms, we accounted for their positional and functional aspect. Genotyping was performed by Real-Time PCR. Multivariable-adjusted general linear and hierarchical regression models were applied. Study subjects (mean age = 40 ± 7 years, 70% who were overweight) had a median dietary omega-6/omega-3 ratio of 16.29. Alternative allele frequencies were 33%, 36%, and 51% for rs174593, rs174576, and rs174616, respectively. Addition of FADS2 alternative alleles was associated with lower plasma arachidonic acid (AA, C20:4 n-6, P < .001) and estimated desaturase-5 activity (P < .001), irrespective of gender, age, daily polyunsaturated/saturated fatty acid intake, and obesity. The rs174576 association with AA withstood multiple testing and additional adjustments for other variants (multivariable-adjusted β = −1.14 [95% CI: −2.25, −0.43]). None of the variants was associated with dietary intake, serum lipids, or obesity. We observed inverse associations between FADS2 variants and plasma AA but not omega-3 fatty acids in Serbian subjects, with rs174576 exhibiting the strongest relation.",
journal = "Nutrition Research",
title = "FADS2 polymorphisms are associated with plasma arachidonic acid and estimated desaturase-5 activity in a cross-sectional study",
volume = "83",
pages = "49-62",
doi = "10.1016/j.nutres.2020.08.010"
}

Zec, M., Stojković, L. S., Zeković, M., Pokimica, B., Živković, M., Stanković, A.,& Glibetić, M.. (2020). FADS2 polymorphisms are associated with plasma arachidonic acid and estimated desaturase-5 activity in a cross-sectional study. in Nutrition Research, 83, 49-62.
https://doi.org/10.1016/j.nutres.2020.08.010

Zec M, Stojković LS, Zeković M, Pokimica B, Živković M, Stanković A, Glibetić M. FADS2 polymorphisms are associated with plasma arachidonic acid and estimated desaturase-5 activity in a cross-sectional study. in Nutrition Research. 2020;83:49-62.
doi:10.1016/j.nutres.2020.08.010 .

Zec, Manja, Stojković, Ljiljana S., Zeković, Milica, Pokimica, Biljana, Živković, Maja, Stanković, Aleksandra, Glibetić, Marija, "FADS2 polymorphisms are associated with plasma arachidonic acid and estimated desaturase-5 activity in a cross-sectional study" in Nutrition Research, 83 (2020):49-62,
https://doi.org/10.1016/j.nutres.2020.08.010 . .

TY  - JOUR
AU  - Zec, Manja M.
AU  - Krga, Irena S.
AU  - Takić, Marija M.
AU  - Debeljak-Martačić, Jasmina D.
AU  - Korićanac, Goran
AU  - Ranković, Slavica G.
AU  - Popović, Tamara B.
AU  - Pantelić, Marija
AU  - Glibetić, Maria D.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9731
AB  - Increased dietary, blood, and tissue n-6/n-3 fatty acid ratios are associated with obesity and metabolic syndrome. Due to Westernized dietary patterns, the increasing n-6/n-3 ratio is of growing concern worldwide, and dietary strategies aimed at its lowering are of public health importance. Walnuts are rich in dietary fats, and their consumption promotes cardiometabolic health. This study aimed to examine the effect of 6-week walnut consumption on tissue-specific n-6/n-3 ratio and fatty acid metabolic conversion in fructose-fed rats with a cluster of metabolic disorders. Male Wistar rats were fed a standard diet with or without 10% fructose in drinking water for 9 weeks. Diets of half of the animals were then supplemented with walnuts (2.4 g/day) for 6 weeks, upon which fatty acid profiles were determined in plasma, liver, adipose tissue, and kidney total lipids. Results showed that walnuts induced significant decreases in the n-6/n-3 content of total lipid pool in plasma and examined tissues, irrespective of metabolic burden. Walnut intervention decreased plasma and liver palmitoleic/palmitic, arachidonic/linoleic, and docosahexaenoic/alpha-linolenic acid ratios. It also modulated individual fatty acid levels by reducing arachidonic and palmitic acid and increasing alpha-linolenic, eicosapentaenoic, and docosapentaenoic acid in plasma and most tissues. Our study demonstrated that 6-week consumption of walnuts favorably modulated n-6/n-3 plasma and tissue ratio in male Wistar rats regardless of high-fructose feeding, underscoring the promising potential of walnuts in both prevention and treatment of the metabolic syndrome.
PB  - Amer Chemical Soc, Washington
T2  - ACS Omega
T1  - Walnut Consumption Induces Tissue-Specific Omega-6/Omega-3 Decrease in High-Fructose-Fed Wistar Rats
VL  - 5
IS  - 43
SP  - 28136
EP  - 28145
DO  - 10.1021/acsomega.0c03784
ER  - 

@article{
author = "Zec, Manja M. and Krga, Irena S. and Takić, Marija M. and Debeljak-Martačić, Jasmina D. and Korićanac, Goran and Ranković, Slavica G. and Popović, Tamara B. and Pantelić, Marija and Glibetić, Maria D.",
year = "2020",
abstract = "Increased dietary, blood, and tissue n-6/n-3 fatty acid ratios are associated with obesity and metabolic syndrome. Due to Westernized dietary patterns, the increasing n-6/n-3 ratio is of growing concern worldwide, and dietary strategies aimed at its lowering are of public health importance. Walnuts are rich in dietary fats, and their consumption promotes cardiometabolic health. This study aimed to examine the effect of 6-week walnut consumption on tissue-specific n-6/n-3 ratio and fatty acid metabolic conversion in fructose-fed rats with a cluster of metabolic disorders. Male Wistar rats were fed a standard diet with or without 10% fructose in drinking water for 9 weeks. Diets of half of the animals were then supplemented with walnuts (2.4 g/day) for 6 weeks, upon which fatty acid profiles were determined in plasma, liver, adipose tissue, and kidney total lipids. Results showed that walnuts induced significant decreases in the n-6/n-3 content of total lipid pool in plasma and examined tissues, irrespective of metabolic burden. Walnut intervention decreased plasma and liver palmitoleic/palmitic, arachidonic/linoleic, and docosahexaenoic/alpha-linolenic acid ratios. It also modulated individual fatty acid levels by reducing arachidonic and palmitic acid and increasing alpha-linolenic, eicosapentaenoic, and docosapentaenoic acid in plasma and most tissues. Our study demonstrated that 6-week consumption of walnuts favorably modulated n-6/n-3 plasma and tissue ratio in male Wistar rats regardless of high-fructose feeding, underscoring the promising potential of walnuts in both prevention and treatment of the metabolic syndrome.",
publisher = "Amer Chemical Soc, Washington",
journal = "ACS Omega",
title = "Walnut Consumption Induces Tissue-Specific Omega-6/Omega-3 Decrease in High-Fructose-Fed Wistar Rats",
volume = "5",
number = "43",
pages = "28136-28145",
doi = "10.1021/acsomega.0c03784"
}

Zec, M. M., Krga, I. S., Takić, M. M., Debeljak-Martačić, J. D., Korićanac, G., Ranković, S. G., Popović, T. B., Pantelić, M.,& Glibetić, M. D.. (2020). Walnut Consumption Induces Tissue-Specific Omega-6/Omega-3 Decrease in High-Fructose-Fed Wistar Rats. in ACS Omega
Amer Chemical Soc, Washington., 5(43), 28136-28145.
https://doi.org/10.1021/acsomega.0c03784

Zec MM, Krga IS, Takić MM, Debeljak-Martačić JD, Korićanac G, Ranković SG, Popović TB, Pantelić M, Glibetić MD. Walnut Consumption Induces Tissue-Specific Omega-6/Omega-3 Decrease in High-Fructose-Fed Wistar Rats. in ACS Omega. 2020;5(43):28136-28145.
doi:10.1021/acsomega.0c03784 .

Zec, Manja M., Krga, Irena S., Takić, Marija M., Debeljak-Martačić, Jasmina D., Korićanac, Goran, Ranković, Slavica G., Popović, Tamara B., Pantelić, Marija, Glibetić, Maria D., "Walnut Consumption Induces Tissue-Specific Omega-6/Omega-3 Decrease in High-Fructose-Fed Wistar Rats" in ACS Omega, 5, no. 43 (2020):28136-28145,
https://doi.org/10.1021/acsomega.0c03784 . .

TY  - JOUR
AU  - Karadzic, Ivana
AU  - Vučić, Vesna
AU  - Jokanović, Vukoman R.
AU  - Debeljak-Martačić, Jasmina
AU  - Marković, Dejan
AU  - Petrović, Snježana
AU  - Glibetić, Marija
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/235
AB  - The aim of this study was to examine the differential capacity of isolated dental pulp stem cells (SHED) cultured onto four different scaffold materials. The differential potential of isolated SHED was examined on the following scaffolds: porous hydroxyapatite (pHAP) alone or combined with three polymers [polylactic-co-glycolic acid (PLGA), alginate, and ethylene vinylacetate / ethylene vinylversatate (EVA/EVV)]. SHED were isolated by outgrowth method and characterized by the flow cytometry. Viability of cells grown with scaffolds was assessed by MTT and LDH assays. No significant cytotoxic effect of any of the tested materials was shown. Staining with alizarin red and estimated alkaline phosphatase activity to identify differentiation, demonstrated osteoblastic phenotype of SHED and newly deposited and mineralized extra cellular matrix (ECM) in presence of all tested scaffolds. The developed ECM seen at scanning electronic micrographs additionally confirmed the osteogenic differentiation and biocompatibility between cells and materials. In summary, all studied biomaterials are suitable carriers for proliferation and osteoblastic differentiation of dental pulp mesenchymal stem cells in vitro. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 350-357, 2015.
T2  - Journal of Biomedical Materials Research. Part A
T1  - Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells
VL  - 103
IS  - 1
SP  - 350
EP  - 357
DO  - 10.1002/jbm.a.35180
ER  - 

@article{
author = "Karadzic, Ivana and Vučić, Vesna and Jokanović, Vukoman R. and Debeljak-Martačić, Jasmina and Marković, Dejan and Petrović, Snježana and Glibetić, Marija",
year = "2015",
abstract = "The aim of this study was to examine the differential capacity of isolated dental pulp stem cells (SHED) cultured onto four different scaffold materials. The differential potential of isolated SHED was examined on the following scaffolds: porous hydroxyapatite (pHAP) alone or combined with three polymers [polylactic-co-glycolic acid (PLGA), alginate, and ethylene vinylacetate / ethylene vinylversatate (EVA/EVV)]. SHED were isolated by outgrowth method and characterized by the flow cytometry. Viability of cells grown with scaffolds was assessed by MTT and LDH assays. No significant cytotoxic effect of any of the tested materials was shown. Staining with alizarin red and estimated alkaline phosphatase activity to identify differentiation, demonstrated osteoblastic phenotype of SHED and newly deposited and mineralized extra cellular matrix (ECM) in presence of all tested scaffolds. The developed ECM seen at scanning electronic micrographs additionally confirmed the osteogenic differentiation and biocompatibility between cells and materials. In summary, all studied biomaterials are suitable carriers for proliferation and osteoblastic differentiation of dental pulp mesenchymal stem cells in vitro. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 350-357, 2015.",
journal = "Journal of Biomedical Materials Research. Part A",
title = "Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells",
volume = "103",
number = "1",
pages = "350-357",
doi = "10.1002/jbm.a.35180"
}

Karadzic, I., Vučić, V., Jokanović, V. R., Debeljak-Martačić, J., Marković, D., Petrović, S.,& Glibetić, M.. (2015). Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells. in Journal of Biomedical Materials Research. Part A, 103(1), 350-357.
https://doi.org/10.1002/jbm.a.35180

Karadzic I, Vučić V, Jokanović VR, Debeljak-Martačić J, Marković D, Petrović S, Glibetić M. Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells. in Journal of Biomedical Materials Research. Part A. 2015;103(1):350-357.
doi:10.1002/jbm.a.35180 .

Karadzic, Ivana, Vučić, Vesna, Jokanović, Vukoman R., Debeljak-Martačić, Jasmina, Marković, Dejan, Petrović, Snježana, Glibetić, Marija, "Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells" in Journal of Biomedical Materials Research. Part A, 103, no. 1 (2015):350-357,
https://doi.org/10.1002/jbm.a.35180 . .

TY  - JOUR
AU  - Petrovic, S.
AU  - Takic, M.
AU  - Arsic, A.
AU  - Vučić, Vesna
AU  - Drakulić, Dunja R.
AU  - Milošević, Maja
AU  - Glibetić, Marija
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6095
AB  - The effects of 8-days treatment with 17 alpha-estradiol (33.3 mu g/kg) and progesterone (1.7 mg/kg) on plasma lipids and fatty acid composition of plasma phospholipids were examined in intact (INT) and bilaterally common carotid arteries occluded (BCO) male Wistar rats. Significant decrease of triglyceride level was found in BCO rats after the estradiol treatment. Both hormones elevated proportion of 18:1n-7 fatty acid in INT, but they failed to have such an effect in BCO. Estradiol increased 22:5n-3 and total n-3 polyunsaturated fatty acids (PUFA) in intact, and decreased 18:2n-6 in BCO rats. Significantly lower level of total n-3 was found in progesterone-treated than in estradiol-treated BCO rats. Given that n-3 PUFA have many beneficial effects on cell and tissue function, while n-6 PUFA have mostly the opposite effects, estradiol, rather than progesterone, was seen to improve plasma lipids and phospholipids FA profiles in INT and BCO animals. Estradiol significantly elevated the estimated activity of Delta 9-desaturases and progesterone of Delta 5-desaturase in BCO group, with no effects in INT rats.
T2  - Physiological Research
T1  - Effect of Sex Hormones on Plasma Phospholipid Fatty Acid Composition in Intact Rats and Rats With Bilaterally Occluded Carotid Arteries
VL  - 63
IS  - 3
SP  - 331
EP  - 339
UR  - https://hdl.handle.net/21.15107/rcub_vinar_6095
ER  - 

@article{
author = "Petrovic, S. and Takic, M. and Arsic, A. and Vučić, Vesna and Drakulić, Dunja R. and Milošević, Maja and Glibetić, Marija",
year = "2014",
abstract = "The effects of 8-days treatment with 17 alpha-estradiol (33.3 mu g/kg) and progesterone (1.7 mg/kg) on plasma lipids and fatty acid composition of plasma phospholipids were examined in intact (INT) and bilaterally common carotid arteries occluded (BCO) male Wistar rats. Significant decrease of triglyceride level was found in BCO rats after the estradiol treatment. Both hormones elevated proportion of 18:1n-7 fatty acid in INT, but they failed to have such an effect in BCO. Estradiol increased 22:5n-3 and total n-3 polyunsaturated fatty acids (PUFA) in intact, and decreased 18:2n-6 in BCO rats. Significantly lower level of total n-3 was found in progesterone-treated than in estradiol-treated BCO rats. Given that n-3 PUFA have many beneficial effects on cell and tissue function, while n-6 PUFA have mostly the opposite effects, estradiol, rather than progesterone, was seen to improve plasma lipids and phospholipids FA profiles in INT and BCO animals. Estradiol significantly elevated the estimated activity of Delta 9-desaturases and progesterone of Delta 5-desaturase in BCO group, with no effects in INT rats.",
journal = "Physiological Research",
title = "Effect of Sex Hormones on Plasma Phospholipid Fatty Acid Composition in Intact Rats and Rats With Bilaterally Occluded Carotid Arteries",
volume = "63",
number = "3",
pages = "331-339",
url = "https://hdl.handle.net/21.15107/rcub_vinar_6095"
}

Petrovic, S., Takic, M., Arsic, A., Vučić, V., Drakulić, D. R., Milošević, M.,& Glibetić, M.. (2014). Effect of Sex Hormones on Plasma Phospholipid Fatty Acid Composition in Intact Rats and Rats With Bilaterally Occluded Carotid Arteries. in Physiological Research, 63(3), 331-339.
https://hdl.handle.net/21.15107/rcub_vinar_6095

Petrovic S, Takic M, Arsic A, Vučić V, Drakulić DR, Milošević M, Glibetić M. Effect of Sex Hormones on Plasma Phospholipid Fatty Acid Composition in Intact Rats and Rats With Bilaterally Occluded Carotid Arteries. in Physiological Research. 2014;63(3):331-339.
https://hdl.handle.net/21.15107/rcub_vinar_6095 .

Petrovic, S., Takic, M., Arsic, A., Vučić, Vesna, Drakulić, Dunja R., Milošević, Maja, Glibetić, Marija, "Effect of Sex Hormones on Plasma Phospholipid Fatty Acid Composition in Intact Rats and Rats With Bilaterally Occluded Carotid Arteries" in Physiological Research, 63, no. 3 (2014):331-339,
https://hdl.handle.net/21.15107/rcub_vinar_6095 .