TY  - JOUR
AU  - Joksić, Gordana
AU  - Filipović Tričković, Jelena G.
AU  - Mićić, Mileva
AU  - Joksić, Ivana
AU  - Valenta-Šobot, Ana
AU  - Demajo, Miroslav
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9729
AB  - Traditional methods in cell proliferation studies are based on immunohistochemical detection of proliferating cells in the target tissue. Since they are time consuming, optimization of novel, more efficient methods is important for large scale proliferation studies. In this study, we aimed to optimize the isolation of single epithelial rat forestomach cells for flow cytometry. As a marker of cellular proliferation we used the Ki-67 antibody to detect this nuclear protein expressed in proliferating cells. We also performed immunohistochemical detection of Ki-67 positive cells and propidium iodide staining to validate the results. 3-tert-butyl-4-hydroxyanisole was used as the positive control to ensure cellular proliferation. The results showed that isolation of epithelial cells with collagenase, trypsin and cell strainer ensures great cell viability (>95%) and the purity of the samples. Flow cytometry and immunostaining with the Ki-67 antibody indicated that 3-tert-butyl-4-hydroxyanisole treatment leads to a significant increase in proliferation. A significant positive correlation was observed between the results obtained by immunohistochemistry and flow cytometry, but the flow cytometric data had a smaller measurement error, suggesting the equal sensitivity and greater accuracy of this method. Propidium iodide staining showed that the percentage of cells in the G2+S phase of the cell cycle correlated positively with the percentage of Ki-67 positive cells assessed by flow cytometry, indicating that Ki-67 positive cells reflect an active dividing cell pool. We conclude that the isolation of forestomach epithelial cells described is a simple and reliable method for obtaining viable cells for use in flow cytometry. Compared to immunohistochemistry, flow cytometric detection of the Ki-67 antigen is equally sensitive, but much faster and provides more accurate results. © 2020, University of Zagreb, Facultty of Veterinary Medicine. All rights reserved.
T2  - Veterinarski Arhiv
T1  - Optimization of the method for isolation of epithelial cells from the non-glandular part of the rat stomach for flow cytometry
VL  - 90
IS  - 5
SP  - 517
EP  - 525
DO  - 10.24099/vet.arhiv.0956
ER  - 

@article{
author = "Joksić, Gordana and Filipović Tričković, Jelena G. and Mićić, Mileva and Joksić, Ivana and Valenta-Šobot, Ana and Demajo, Miroslav",
year = "2020",
abstract = "Traditional methods in cell proliferation studies are based on immunohistochemical detection of proliferating cells in the target tissue. Since they are time consuming, optimization of novel, more efficient methods is important for large scale proliferation studies. In this study, we aimed to optimize the isolation of single epithelial rat forestomach cells for flow cytometry. As a marker of cellular proliferation we used the Ki-67 antibody to detect this nuclear protein expressed in proliferating cells. We also performed immunohistochemical detection of Ki-67 positive cells and propidium iodide staining to validate the results. 3-tert-butyl-4-hydroxyanisole was used as the positive control to ensure cellular proliferation. The results showed that isolation of epithelial cells with collagenase, trypsin and cell strainer ensures great cell viability (>95%) and the purity of the samples. Flow cytometry and immunostaining with the Ki-67 antibody indicated that 3-tert-butyl-4-hydroxyanisole treatment leads to a significant increase in proliferation. A significant positive correlation was observed between the results obtained by immunohistochemistry and flow cytometry, but the flow cytometric data had a smaller measurement error, suggesting the equal sensitivity and greater accuracy of this method. Propidium iodide staining showed that the percentage of cells in the G2+S phase of the cell cycle correlated positively with the percentage of Ki-67 positive cells assessed by flow cytometry, indicating that Ki-67 positive cells reflect an active dividing cell pool. We conclude that the isolation of forestomach epithelial cells described is a simple and reliable method for obtaining viable cells for use in flow cytometry. Compared to immunohistochemistry, flow cytometric detection of the Ki-67 antigen is equally sensitive, but much faster and provides more accurate results. © 2020, University of Zagreb, Facultty of Veterinary Medicine. All rights reserved.",
journal = "Veterinarski Arhiv",
title = "Optimization of the method for isolation of epithelial cells from the non-glandular part of the rat stomach for flow cytometry",
volume = "90",
number = "5",
pages = "517-525",
doi = "10.24099/vet.arhiv.0956"
}

Joksić, G., Filipović Tričković, J. G., Mićić, M., Joksić, I., Valenta-Šobot, A.,& Demajo, M.. (2020). Optimization of the method for isolation of epithelial cells from the non-glandular part of the rat stomach for flow cytometry. in Veterinarski Arhiv, 90(5), 517-525.
https://doi.org/10.24099/vet.arhiv.0956

Joksić G, Filipović Tričković JG, Mićić M, Joksić I, Valenta-Šobot A, Demajo M. Optimization of the method for isolation of epithelial cells from the non-glandular part of the rat stomach for flow cytometry. in Veterinarski Arhiv. 2020;90(5):517-525.
doi:10.24099/vet.arhiv.0956 .

Joksić, Gordana, Filipović Tričković, Jelena G., Mićić, Mileva, Joksić, Ivana, Valenta-Šobot, Ana, Demajo, Miroslav, "Optimization of the method for isolation of epithelial cells from the non-glandular part of the rat stomach for flow cytometry" in Veterinarski Arhiv, 90, no. 5 (2020):517-525,
https://doi.org/10.24099/vet.arhiv.0956 . .

TY  - JOUR
AU  - Joksić, Gordana
AU  - Filipović Tričković, Jelena G.
AU  - Joksić, Ivana
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8516
AB  - Background: Obesity, diabetes, and associated diseases are increasing all over the world, and pose a great burden on public health. According to the latest reports, 440 million people are suffering from diabetes. Diabetes is caused by impaired ability to produce or respond to the hormone insulin consequently resulting in hyperglycemia. Methods: Data used for this review was obtained by using PUBMED/MEDLINE (1987-2018). The main data search terms were: Gentiana lutea, Gentiana lutea extract, Gentiana lutea constituents, obesity, diabetes mellitus, diabetic complications. Results: In the present review, we describe the potential of root powder of yellow gentian (Gentiana lutea) for the prevention of obesity and diabetes including complications related to this disease. Conclusion: Reasonably effective, low-cost alternatives could fulfill an important role for a large part of the human population and could be of great value for the food market. Even a modest reduction of morbidity and mortality with respect to this disease translates into millions of lives saved. © 2019 Bentham Science Publishers.
T2  - Current Pharmaceutical Design
T1  - Potential of Gentiana lutea for the Treatment of Obesity-associated Diseases
VL  - 25
IS  - 18
SP  - 2071
EP  - 2076
DO  - 10.2174/1381612825666190708215743
ER  - 

@article{
author = "Joksić, Gordana and Filipović Tričković, Jelena G. and Joksić, Ivana",
year = "2019",
abstract = "Background: Obesity, diabetes, and associated diseases are increasing all over the world, and pose a great burden on public health. According to the latest reports, 440 million people are suffering from diabetes. Diabetes is caused by impaired ability to produce or respond to the hormone insulin consequently resulting in hyperglycemia. Methods: Data used for this review was obtained by using PUBMED/MEDLINE (1987-2018). The main data search terms were: Gentiana lutea, Gentiana lutea extract, Gentiana lutea constituents, obesity, diabetes mellitus, diabetic complications. Results: In the present review, we describe the potential of root powder of yellow gentian (Gentiana lutea) for the prevention of obesity and diabetes including complications related to this disease. Conclusion: Reasonably effective, low-cost alternatives could fulfill an important role for a large part of the human population and could be of great value for the food market. Even a modest reduction of morbidity and mortality with respect to this disease translates into millions of lives saved. © 2019 Bentham Science Publishers.",
journal = "Current Pharmaceutical Design",
title = "Potential of Gentiana lutea for the Treatment of Obesity-associated Diseases",
volume = "25",
number = "18",
pages = "2071-2076",
doi = "10.2174/1381612825666190708215743"
}

Joksić, G., Filipović Tričković, J. G.,& Joksić, I.. (2019). Potential of Gentiana lutea for the Treatment of Obesity-associated Diseases. in Current Pharmaceutical Design, 25(18), 2071-2076.
https://doi.org/10.2174/1381612825666190708215743

Joksić G, Filipović Tričković JG, Joksić I. Potential of Gentiana lutea for the Treatment of Obesity-associated Diseases. in Current Pharmaceutical Design. 2019;25(18):2071-2076.
doi:10.2174/1381612825666190708215743 .

Joksić, Gordana, Filipović Tričković, Jelena G., Joksić, Ivana, "Potential of Gentiana lutea for the Treatment of Obesity-associated Diseases" in Current Pharmaceutical Design, 25, no. 18 (2019):2071-2076,
https://doi.org/10.2174/1381612825666190708215743 . .

TY  - JOUR
AU  - Filipović Tričković, Jelena G.
AU  - Mandušić, Vesna
AU  - Joksić, Ivana
AU  - Vujić, Dragana
AU  - Valenta-Šobot, Ana
AU  - Joksić, Gordana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1882
AB  - Fanconi anemia is rare inherited disease characterized by wide spectrum of congenital anomalies, progressive pancytopenia, and predisposition to hematological malignancies and solid tumors. Molecular genetic analysis of mutations in FANC genes is of a great importance for diagnosis confirmation, prenatal and carrier testing, as well as for prediction of chemotherapy outcome and disease complications. In this study we performed screening of frequently affected regions of FANCD2 gene for sequence variants in six unrelated FA-D2 patients in Serbia. This is the first molecular analysis of FANCD2 gene in Serbian FA-D2 patients. A total of 10 sequence variants were detected, one in homozygous, and nine in heterozygous state. Two variants were found within exons, and eight within introns, in deep intronic regions. In-silico analysis showed that among all detected variants one exon variant and three intron variants might have impact on splicing mechanism. Heterozygous variants found in intron 3, c. 206-246delG; exon 26, c. 2396 C GT A and intron 28, c. 2715+573 C GT T were not previously reported. In-silico analysis revealed that among them, two (intron 3, c. 206-246 delG and exon 26, c. 2396 C GT A) could be novel disease-causing mutations. Many variants were found in more than one patient, including those unreported, indicating their possible ethnic association. Great number of variants in some patients suggests their non-random emergence in Fanconi anemia pathway.
T2  - Genetika
T1  - Genotyping Fanconi Anemia Patients from Serbia Reveals Three Novel Fancd2 Variants
VL  - 49
IS  - 2
SP  - 559
EP  - 572
DO  - 10.2298/GENSR1702559T
ER  - 

@article{
author = "Filipović Tričković, Jelena G. and Mandušić, Vesna and Joksić, Ivana and Vujić, Dragana and Valenta-Šobot, Ana and Joksić, Gordana",
year = "2017",
abstract = "Fanconi anemia is rare inherited disease characterized by wide spectrum of congenital anomalies, progressive pancytopenia, and predisposition to hematological malignancies and solid tumors. Molecular genetic analysis of mutations in FANC genes is of a great importance for diagnosis confirmation, prenatal and carrier testing, as well as for prediction of chemotherapy outcome and disease complications. In this study we performed screening of frequently affected regions of FANCD2 gene for sequence variants in six unrelated FA-D2 patients in Serbia. This is the first molecular analysis of FANCD2 gene in Serbian FA-D2 patients. A total of 10 sequence variants were detected, one in homozygous, and nine in heterozygous state. Two variants were found within exons, and eight within introns, in deep intronic regions. In-silico analysis showed that among all detected variants one exon variant and three intron variants might have impact on splicing mechanism. Heterozygous variants found in intron 3, c. 206-246delG; exon 26, c. 2396 C GT A and intron 28, c. 2715+573 C GT T were not previously reported. In-silico analysis revealed that among them, two (intron 3, c. 206-246 delG and exon 26, c. 2396 C GT A) could be novel disease-causing mutations. Many variants were found in more than one patient, including those unreported, indicating their possible ethnic association. Great number of variants in some patients suggests their non-random emergence in Fanconi anemia pathway.",
journal = "Genetika",
title = "Genotyping Fanconi Anemia Patients from Serbia Reveals Three Novel Fancd2 Variants",
volume = "49",
number = "2",
pages = "559-572",
doi = "10.2298/GENSR1702559T"
}

Filipović Tričković, J. G., Mandušić, V., Joksić, I., Vujić, D., Valenta-Šobot, A.,& Joksić, G.. (2017). Genotyping Fanconi Anemia Patients from Serbia Reveals Three Novel Fancd2 Variants. in Genetika, 49(2), 559-572.
https://doi.org/10.2298/GENSR1702559T

Filipović Tričković JG, Mandušić V, Joksić I, Vujić D, Valenta-Šobot A, Joksić G. Genotyping Fanconi Anemia Patients from Serbia Reveals Three Novel Fancd2 Variants. in Genetika. 2017;49(2):559-572.
doi:10.2298/GENSR1702559T .

Filipović Tričković, Jelena G., Mandušić, Vesna, Joksić, Ivana, Vujić, Dragana, Valenta-Šobot, Ana, Joksić, Gordana, "Genotyping Fanconi Anemia Patients from Serbia Reveals Three Novel Fancd2 Variants" in Genetika, 49, no. 2 (2017):559-572,
https://doi.org/10.2298/GENSR1702559T . .

TY  - JOUR
AU  - Filipović, Jelena G.
AU  - Joksić, Gordana
AU  - Vujić, Dragana
AU  - Joksić, Ivana
AU  - Mrasek, Kristin
AU  - Weise, Anja
AU  - Liehr, Thomas
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1239
AB  - Background: Fanconi anemia (FA) is a chromosomal instability syndrome characterized by increased frequency of chromosomal breakages, chromosomal radial figures and accelerated telomere shortening. In this work we performed detailed molecular-cytogenetic characterization of breakpoints in primary lymphocytes of FA-D2 patients in different stages of the disease using fluorescent in situ hybridization. Results: We found that chromosomal breakpoints co-localize on the molecular level with common fragile sites, whereas their distribution pattern depends on the severity of the disease. Telomere quantitative fluorescent in situ hybridization revealed that telomere fusions and radial figures, especially radials which involve telomere sequences are the consequence of critically shortened telomeres that increase with the disease progression and could be considered as a predictive parameter during the course of the disease. Sex chromosomes in FA cells are also involved in radial formation indicating that specific X chromosome regions share homology with autosomes and also could serve as repair templates in resolving DNA damage. Conclusions: FA-D2 chromosomal breakpoints co-localize with common fragile sites, but their distribution pattern depends on the disease stage. Telomere fusions and radials figures which involve telomere sequences are the consequence of shortened telomeres, increase with disease progression and could be of predictive value.
T2  - Molecular Cytogenetics
T1  - First molecular-cytogenetic characterization of Fanconi anemia fragile sites in primary lymphocytes of FA-D2 patients in different stages of the disease
VL  - 9
DO  - 10.1186/s13039-016-0280-6
ER  - 

@article{
author = "Filipović, Jelena G. and Joksić, Gordana and Vujić, Dragana and Joksić, Ivana and Mrasek, Kristin and Weise, Anja and Liehr, Thomas",
year = "2016",
abstract = "Background: Fanconi anemia (FA) is a chromosomal instability syndrome characterized by increased frequency of chromosomal breakages, chromosomal radial figures and accelerated telomere shortening. In this work we performed detailed molecular-cytogenetic characterization of breakpoints in primary lymphocytes of FA-D2 patients in different stages of the disease using fluorescent in situ hybridization. Results: We found that chromosomal breakpoints co-localize on the molecular level with common fragile sites, whereas their distribution pattern depends on the severity of the disease. Telomere quantitative fluorescent in situ hybridization revealed that telomere fusions and radial figures, especially radials which involve telomere sequences are the consequence of critically shortened telomeres that increase with the disease progression and could be considered as a predictive parameter during the course of the disease. Sex chromosomes in FA cells are also involved in radial formation indicating that specific X chromosome regions share homology with autosomes and also could serve as repair templates in resolving DNA damage. Conclusions: FA-D2 chromosomal breakpoints co-localize with common fragile sites, but their distribution pattern depends on the disease stage. Telomere fusions and radials figures which involve telomere sequences are the consequence of shortened telomeres, increase with disease progression and could be of predictive value.",
journal = "Molecular Cytogenetics",
title = "First molecular-cytogenetic characterization of Fanconi anemia fragile sites in primary lymphocytes of FA-D2 patients in different stages of the disease",
volume = "9",
doi = "10.1186/s13039-016-0280-6"
}

Filipović, J. G., Joksić, G., Vujić, D., Joksić, I., Mrasek, K., Weise, A.,& Liehr, T.. (2016). First molecular-cytogenetic characterization of Fanconi anemia fragile sites in primary lymphocytes of FA-D2 patients in different stages of the disease. in Molecular Cytogenetics, 9.
https://doi.org/10.1186/s13039-016-0280-6

Filipović JG, Joksić G, Vujić D, Joksić I, Mrasek K, Weise A, Liehr T. First molecular-cytogenetic characterization of Fanconi anemia fragile sites in primary lymphocytes of FA-D2 patients in different stages of the disease. in Molecular Cytogenetics. 2016;9.
doi:10.1186/s13039-016-0280-6 .

Filipović, Jelena G., Joksić, Gordana, Vujić, Dragana, Joksić, Ivana, Mrasek, Kristin, Weise, Anja, Liehr, Thomas, "First molecular-cytogenetic characterization of Fanconi anemia fragile sites in primary lymphocytes of FA-D2 patients in different stages of the disease" in Molecular Cytogenetics, 9 (2016),
https://doi.org/10.1186/s13039-016-0280-6 . .

TY  - JOUR
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Joksić, Ivana
AU  - Vujić, Dragana
AU  - Valenta-Šobot, Ana
AU  - Filipović, Jelena G.
AU  - Joksić, Gordana
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/777
AB  - Fanconi anemia (FA) is a rare genetically heterogeneous disorder associated with bone marrow failure, birth defects and cancer susceptibility. Apart from the disease-causing mutations in FANC genes, the identification of specific DNA variations, such as single nucleotide polymorphisms (SNPs), in other candidate genes may lead to a better clinical description of this condition enabling individualized treatment with improvement of the prognosis. In this study, we have assessed 95 SNPs located in 52 key genes involved in base excision repair (BER), nucleotide excision repair (NER), mismatch repair (MMR), double strand break (DSB) repair and cell cycle control using a DNA repair chip (Asper Biotech, Estonia) which includes most of the common variants for the candidate genes. The SNP genotyping was performed in five FA-D2 patients and in one FA-A patient. The polymorphisms studied were synonymous (n=10), nonsynonymous (missense) (n=52) and in non-coding regions of the genome (introns and 5 and 3 untranslated regions (UTR)) (n=33). Polymorphisms found at the homozygous state are selected for further analysis. Our results have shown a significant inter-individual variability among patients in the type and the frequency of SNPs and also elucidate the need for further studies of polymorphisms located in ATM, APEX APE 1, XRCC1, ERCC2, MSH3, PARP4, NBS1, BARD1, CDKN1B, TP53 and TP53BP1 which may be of great importance for better clinical description of FA. In addition, the present report recommends the use of SNPs as predictive and prognostic genetic markers to individualize therapy of FA patients.
T2  - Genetika
T1  - Assessment of Single Nucleotide Polymorphisms in Screening 52 DNA Repair and Cell Cycle Control Genes in Fanconi Anemia Patients
VL  - 47
IS  - 2
SP  - 695
EP  - 710
DO  - 10.2298/GENSR1502695P
ER  - 

@article{
author = "Petrović, Sandra and Leskovac, Andreja and Joksić, Ivana and Vujić, Dragana and Valenta-Šobot, Ana and Filipović, Jelena G. and Joksić, Gordana",
year = "2015",
abstract = "Fanconi anemia (FA) is a rare genetically heterogeneous disorder associated with bone marrow failure, birth defects and cancer susceptibility. Apart from the disease-causing mutations in FANC genes, the identification of specific DNA variations, such as single nucleotide polymorphisms (SNPs), in other candidate genes may lead to a better clinical description of this condition enabling individualized treatment with improvement of the prognosis. In this study, we have assessed 95 SNPs located in 52 key genes involved in base excision repair (BER), nucleotide excision repair (NER), mismatch repair (MMR), double strand break (DSB) repair and cell cycle control using a DNA repair chip (Asper Biotech, Estonia) which includes most of the common variants for the candidate genes. The SNP genotyping was performed in five FA-D2 patients and in one FA-A patient. The polymorphisms studied were synonymous (n=10), nonsynonymous (missense) (n=52) and in non-coding regions of the genome (introns and 5 and 3 untranslated regions (UTR)) (n=33). Polymorphisms found at the homozygous state are selected for further analysis. Our results have shown a significant inter-individual variability among patients in the type and the frequency of SNPs and also elucidate the need for further studies of polymorphisms located in ATM, APEX APE 1, XRCC1, ERCC2, MSH3, PARP4, NBS1, BARD1, CDKN1B, TP53 and TP53BP1 which may be of great importance for better clinical description of FA. In addition, the present report recommends the use of SNPs as predictive and prognostic genetic markers to individualize therapy of FA patients.",
journal = "Genetika",
title = "Assessment of Single Nucleotide Polymorphisms in Screening 52 DNA Repair and Cell Cycle Control Genes in Fanconi Anemia Patients",
volume = "47",
number = "2",
pages = "695-710",
doi = "10.2298/GENSR1502695P"
}

Petrović, S., Leskovac, A., Joksić, I., Vujić, D., Valenta-Šobot, A., Filipović, J. G.,& Joksić, G.. (2015). Assessment of Single Nucleotide Polymorphisms in Screening 52 DNA Repair and Cell Cycle Control Genes in Fanconi Anemia Patients. in Genetika, 47(2), 695-710.
https://doi.org/10.2298/GENSR1502695P

Petrović S, Leskovac A, Joksić I, Vujić D, Valenta-Šobot A, Filipović JG, Joksić G. Assessment of Single Nucleotide Polymorphisms in Screening 52 DNA Repair and Cell Cycle Control Genes in Fanconi Anemia Patients. in Genetika. 2015;47(2):695-710.
doi:10.2298/GENSR1502695P .

Petrović, Sandra, Leskovac, Andreja, Joksić, Ivana, Vujić, Dragana, Valenta-Šobot, Ana, Filipović, Jelena G., Joksić, Gordana, "Assessment of Single Nucleotide Polymorphisms in Screening 52 DNA Repair and Cell Cycle Control Genes in Fanconi Anemia Patients" in Genetika, 47, no. 2 (2015):695-710,
https://doi.org/10.2298/GENSR1502695P . .

TY  - JOUR
AU  - Vujić, Dragana
AU  - Petrović, Sandra
AU  - Lazić, Emilija
AU  - Kuzmanović, Miloš
AU  - Leskovac, Andreja
AU  - Joksić, Ivana
AU  - Mićić, Dragan
AU  - Jovanović, Ankica
AU  - Zečević, Željko
AU  - Guć-Šćekić, Marija
AU  - Ćirković, Sanja
AU  - Joksić, Gordana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5928
AB  - To investigate genetic subtypes of inherited bone marrow failure syndrome Fanconi anemia (FA) in Sebia. FA-D2 subtype was found to be the most frequent genetic subtype among investigated FA patients; specific observations of FA-D2 phenotype are pointed out. Several biological endpoints of FA cells in vitro such as radiation-induced level of lymphocyte micronuclei (radiosensitivity), base line and radiation induced level of the DNA double strand breaks (DSBs), leukocyte apoptosis, and telomere capping function were assessed. The results indicate that all FA-D2 patients display radioresistant in vitro response, which is seen as significantly reduced yield of radiation-induced micronuclei. On the contrary, FA-A patients display radiosensitive in vitro response seen as increased number of radiation-induced micronuclei (MN). A massive elimination of irradiated cells via apoptosis is found in both FA-A and FA-D2 subtypes. In FA-A subtype apoptosis positively relates with the yield of radiation-induced MN, whereas in FA-D2 subtype apoptosis relates with a high percentage of cells carrying dysfunctional telomeres. The present results unequivocally demonstrate that cytokinesis-block micronucleus (CBMN) assay and analyses of telomere capping function can be used to distinguish FA-D2 and FA-A complementation groups. Considering all biological endpoints were analyzed, it can be concluded that all FA patients are radiosensitive, regardless of their complementation group. Thus, using CBMN test and telomere capping function analysis can discriminate FA-A from FA-D2 complementation groups, which could be important for assessment the conditioning regimens prior to bone marrow transplantation.
T2  - Indian Journal of Pediatrics
T1  - Prevalence of FA-D2 Rare Complementation Group of Fanconi Anemia in Serbia
VL  - 81
IS  - 3
SP  - 260
EP  - 265
DO  - 10.1007/s12098-013-1284-4
ER  - 

@article{
author = "Vujić, Dragana and Petrović, Sandra and Lazić, Emilija and Kuzmanović, Miloš and Leskovac, Andreja and Joksić, Ivana and Mićić, Dragan and Jovanović, Ankica and Zečević, Željko and Guć-Šćekić, Marija and Ćirković, Sanja and Joksić, Gordana",
year = "2014",
abstract = "To investigate genetic subtypes of inherited bone marrow failure syndrome Fanconi anemia (FA) in Sebia. FA-D2 subtype was found to be the most frequent genetic subtype among investigated FA patients; specific observations of FA-D2 phenotype are pointed out. Several biological endpoints of FA cells in vitro such as radiation-induced level of lymphocyte micronuclei (radiosensitivity), base line and radiation induced level of the DNA double strand breaks (DSBs), leukocyte apoptosis, and telomere capping function were assessed. The results indicate that all FA-D2 patients display radioresistant in vitro response, which is seen as significantly reduced yield of radiation-induced micronuclei. On the contrary, FA-A patients display radiosensitive in vitro response seen as increased number of radiation-induced micronuclei (MN). A massive elimination of irradiated cells via apoptosis is found in both FA-A and FA-D2 subtypes. In FA-A subtype apoptosis positively relates with the yield of radiation-induced MN, whereas in FA-D2 subtype apoptosis relates with a high percentage of cells carrying dysfunctional telomeres. The present results unequivocally demonstrate that cytokinesis-block micronucleus (CBMN) assay and analyses of telomere capping function can be used to distinguish FA-D2 and FA-A complementation groups. Considering all biological endpoints were analyzed, it can be concluded that all FA patients are radiosensitive, regardless of their complementation group. Thus, using CBMN test and telomere capping function analysis can discriminate FA-A from FA-D2 complementation groups, which could be important for assessment the conditioning regimens prior to bone marrow transplantation.",
journal = "Indian Journal of Pediatrics",
title = "Prevalence of FA-D2 Rare Complementation Group of Fanconi Anemia in Serbia",
volume = "81",
number = "3",
pages = "260-265",
doi = "10.1007/s12098-013-1284-4"
}

Vujić, D., Petrović, S., Lazić, E., Kuzmanović, M., Leskovac, A., Joksić, I., Mićić, D., Jovanović, A., Zečević, Ž., Guć-Šćekić, M., Ćirković, S.,& Joksić, G.. (2014). Prevalence of FA-D2 Rare Complementation Group of Fanconi Anemia in Serbia. in Indian Journal of Pediatrics, 81(3), 260-265.
https://doi.org/10.1007/s12098-013-1284-4

Vujić D, Petrović S, Lazić E, Kuzmanović M, Leskovac A, Joksić I, Mićić D, Jovanović A, Zečević Ž, Guć-Šćekić M, Ćirković S, Joksić G. Prevalence of FA-D2 Rare Complementation Group of Fanconi Anemia in Serbia. in Indian Journal of Pediatrics. 2014;81(3):260-265.
doi:10.1007/s12098-013-1284-4 .

Vujić, Dragana, Petrović, Sandra, Lazić, Emilija, Kuzmanović, Miloš, Leskovac, Andreja, Joksić, Ivana, Mićić, Dragan, Jovanović, Ankica, Zečević, Željko, Guć-Šćekić, Marija, Ćirković, Sanja, Joksić, Gordana, "Prevalence of FA-D2 Rare Complementation Group of Fanconi Anemia in Serbia" in Indian Journal of Pediatrics, 81, no. 3 (2014):260-265,
https://doi.org/10.1007/s12098-013-1284-4 . .

TY  - JOUR
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Joksić, Ivana
AU  - Filipović, Jelena G.
AU  - Vujić, Dragana
AU  - Guć-Šćekić, Marija
AU  - Joksić, Gordana
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5606
AB  - Fanconi anemia (FA) is a rare cancer-prone genetic disease characterized by impaired oxygen metabolism and defects in DNA damage repair. Response of FA cells to ionizing radiation has been an issue intensively debated in the literature. To study in vitro radiosensitivity in patients suffering from FA and their parents (heterozygous carriers), we determined radiation-induced leukocyte apoptosis using flow cytometry. As TP53 gene is involved in the control of apoptosis, we studied its status in FA lymphocytes using dual colour fluorescence in situ hybridization (FISH). FA patients and female heterozygous carriers display radiosensitive response to ionizing radiation seen as abnormal elimination of cells via apoptosis. By employment of FISH, the TP53 allele loss in FA lymphocytes was not observed. In diseases related to oxidative stress, determination of radiation-induced apoptosis is the method of choice for testing the radiosensitivity.
T2  - Current Science
T1  - Leukocyte apoptosis as a predictor of radiosensitivity in Fanconi anemia
VL  - 105
IS  - 1
SP  - 56
EP  - 60
UR  - https://hdl.handle.net/21.15107/rcub_vinar_5606
ER  - 

@article{
author = "Petrović, Sandra and Leskovac, Andreja and Joksić, Ivana and Filipović, Jelena G. and Vujić, Dragana and Guć-Šćekić, Marija and Joksić, Gordana",
year = "2013",
abstract = "Fanconi anemia (FA) is a rare cancer-prone genetic disease characterized by impaired oxygen metabolism and defects in DNA damage repair. Response of FA cells to ionizing radiation has been an issue intensively debated in the literature. To study in vitro radiosensitivity in patients suffering from FA and their parents (heterozygous carriers), we determined radiation-induced leukocyte apoptosis using flow cytometry. As TP53 gene is involved in the control of apoptosis, we studied its status in FA lymphocytes using dual colour fluorescence in situ hybridization (FISH). FA patients and female heterozygous carriers display radiosensitive response to ionizing radiation seen as abnormal elimination of cells via apoptosis. By employment of FISH, the TP53 allele loss in FA lymphocytes was not observed. In diseases related to oxidative stress, determination of radiation-induced apoptosis is the method of choice for testing the radiosensitivity.",
journal = "Current Science",
title = "Leukocyte apoptosis as a predictor of radiosensitivity in Fanconi anemia",
volume = "105",
number = "1",
pages = "56-60",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5606"
}

Petrović, S., Leskovac, A., Joksić, I., Filipović, J. G., Vujić, D., Guć-Šćekić, M.,& Joksić, G.. (2013). Leukocyte apoptosis as a predictor of radiosensitivity in Fanconi anemia. in Current Science, 105(1), 56-60.
https://hdl.handle.net/21.15107/rcub_vinar_5606

Petrović S, Leskovac A, Joksić I, Filipović JG, Vujić D, Guć-Šćekić M, Joksić G. Leukocyte apoptosis as a predictor of radiosensitivity in Fanconi anemia. in Current Science. 2013;105(1):56-60.
https://hdl.handle.net/21.15107/rcub_vinar_5606 .

Petrović, Sandra, Leskovac, Andreja, Joksić, Ivana, Filipović, Jelena G., Vujić, Dragana, Guć-Šćekić, Marija, Joksić, Gordana, "Leukocyte apoptosis as a predictor of radiosensitivity in Fanconi anemia" in Current Science, 105, no. 1 (2013):56-60,
https://hdl.handle.net/21.15107/rcub_vinar_5606 .

TY  - JOUR
AU  - Joksić, Ivana
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Filipović, Jelena G.
AU  - Guć-Šćekić, Marija
AU  - Vujić, Dragana
AU  - Joksić, Gordana
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5798
AB  - Fanconi anemia (FA) is a rare genetically heterogeneous disease characterized by developmental abnormalities, progressive bone marrow failure, and cancer susceptibility. We examined spontaneous, diepoxybutane (DEB)-induced and radiation-induced sister chromatid exchanges (SCEs) in wholeblood lymphocyte cultures of bone marrow failure (BMF) patients including Fanconi anemia, mothers of affected individuals, and healthy controls. The baseline frequency of SCE in FA cells was similar to that observed in controls. However, in response to DEB SCE frequencies in FA patients and their mothers were significantly increased compared to both non-FA BMF families and healthy controls. In response to ionizing radiation, cells displayed increased frequency of SCE, but no differences between FA patients and non-FA BMF patients were seen. Our data confirm and expand previous findings by showing that SCE induced by DEB can be used as an adjunct diagnostic test not only for FA patients, but also for female heterozygous carriers, at least for complementation groups FANCA and FANCD2.
T2  - Genetika
T1  - Enhanced Frequency of Sister Chromatid Exchanges Induced By Diepoxybutane Is Specific Characteristic of Fanconi Anemia Cellular Phenotype
VL  - 45
IS  - 2
SP  - 393
EP  - 403
DO  - 10.2298/GENSR1302393J
ER  - 

@article{
author = "Joksić, Ivana and Petrović, Sandra and Leskovac, Andreja and Filipović, Jelena G. and Guć-Šćekić, Marija and Vujić, Dragana and Joksić, Gordana",
year = "2013",
abstract = "Fanconi anemia (FA) is a rare genetically heterogeneous disease characterized by developmental abnormalities, progressive bone marrow failure, and cancer susceptibility. We examined spontaneous, diepoxybutane (DEB)-induced and radiation-induced sister chromatid exchanges (SCEs) in wholeblood lymphocyte cultures of bone marrow failure (BMF) patients including Fanconi anemia, mothers of affected individuals, and healthy controls. The baseline frequency of SCE in FA cells was similar to that observed in controls. However, in response to DEB SCE frequencies in FA patients and their mothers were significantly increased compared to both non-FA BMF families and healthy controls. In response to ionizing radiation, cells displayed increased frequency of SCE, but no differences between FA patients and non-FA BMF patients were seen. Our data confirm and expand previous findings by showing that SCE induced by DEB can be used as an adjunct diagnostic test not only for FA patients, but also for female heterozygous carriers, at least for complementation groups FANCA and FANCD2.",
journal = "Genetika",
title = "Enhanced Frequency of Sister Chromatid Exchanges Induced By Diepoxybutane Is Specific Characteristic of Fanconi Anemia Cellular Phenotype",
volume = "45",
number = "2",
pages = "393-403",
doi = "10.2298/GENSR1302393J"
}

Joksić, I., Petrović, S., Leskovac, A., Filipović, J. G., Guć-Šćekić, M., Vujić, D.,& Joksić, G.. (2013). Enhanced Frequency of Sister Chromatid Exchanges Induced By Diepoxybutane Is Specific Characteristic of Fanconi Anemia Cellular Phenotype. in Genetika, 45(2), 393-403.
https://doi.org/10.2298/GENSR1302393J

Joksić I, Petrović S, Leskovac A, Filipović JG, Guć-Šćekić M, Vujić D, Joksić G. Enhanced Frequency of Sister Chromatid Exchanges Induced By Diepoxybutane Is Specific Characteristic of Fanconi Anemia Cellular Phenotype. in Genetika. 2013;45(2):393-403.
doi:10.2298/GENSR1302393J .

Joksić, Ivana, Petrović, Sandra, Leskovac, Andreja, Filipović, Jelena G., Guć-Šćekić, Marija, Vujić, Dragana, Joksić, Gordana, "Enhanced Frequency of Sister Chromatid Exchanges Induced By Diepoxybutane Is Specific Characteristic of Fanconi Anemia Cellular Phenotype" in Genetika, 45, no. 2 (2013):393-403,
https://doi.org/10.2298/GENSR1302393J . .

TY  - JOUR
AU  - Vujić, Dragana
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Joksić, Ivana
AU  - Filipović, Jelena G.
AU  - Valenta-Šobot, Ana
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5595
AB  - In this paper we present the data of lymphocyte radiosensitivity testing used for characterization of radiosensitive cellular phenotype and diagnostics of ataxia-telangiectasia disease. We point out the advantage of lymphocyte micronucleus test (CBMN) over other cellular tests for assessment of radiosensitivity: the first advantage of CBMN is that primary patient cells are used (less than 1 ml), the second one is that the results of testing are obtained within 3 days and there is no need for establishing a patient-derived cell line, which requires additional time and application of more expensive methods. The third advantage of CBMN method is that it gives information about proliferative ability of cells, which can recognize dysfunctional ataxia-telangiectasia mutated protein. The results are fast and accurate in diagnostics of ataxia-telagiectasia diseases.
T2  - Nuclear technology and radiation protection
T1  - Accurate Diagnostics of Ataxia-Telangiectasia Cellular Phenotype By Employing in Vitro Lymphocyte Radiosensitivity Testing
VL  - 28
IS  - 2
SP  - 221
EP  - 224
DO  - 10.2298/NTRP1302221V
ER  - 

@article{
author = "Vujić, Dragana and Petrović, Sandra and Leskovac, Andreja and Joksić, Ivana and Filipović, Jelena G. and Valenta-Šobot, Ana",
year = "2013",
abstract = "In this paper we present the data of lymphocyte radiosensitivity testing used for characterization of radiosensitive cellular phenotype and diagnostics of ataxia-telangiectasia disease. We point out the advantage of lymphocyte micronucleus test (CBMN) over other cellular tests for assessment of radiosensitivity: the first advantage of CBMN is that primary patient cells are used (less than 1 ml), the second one is that the results of testing are obtained within 3 days and there is no need for establishing a patient-derived cell line, which requires additional time and application of more expensive methods. The third advantage of CBMN method is that it gives information about proliferative ability of cells, which can recognize dysfunctional ataxia-telangiectasia mutated protein. The results are fast and accurate in diagnostics of ataxia-telagiectasia diseases.",
journal = "Nuclear technology and radiation protection",
title = "Accurate Diagnostics of Ataxia-Telangiectasia Cellular Phenotype By Employing in Vitro Lymphocyte Radiosensitivity Testing",
volume = "28",
number = "2",
pages = "221-224",
doi = "10.2298/NTRP1302221V"
}

Vujić, D., Petrović, S., Leskovac, A., Joksić, I., Filipović, J. G.,& Valenta-Šobot, A.. (2013). Accurate Diagnostics of Ataxia-Telangiectasia Cellular Phenotype By Employing in Vitro Lymphocyte Radiosensitivity Testing. in Nuclear technology and radiation protection, 28(2), 221-224.
https://doi.org/10.2298/NTRP1302221V

Vujić D, Petrović S, Leskovac A, Joksić I, Filipović JG, Valenta-Šobot A. Accurate Diagnostics of Ataxia-Telangiectasia Cellular Phenotype By Employing in Vitro Lymphocyte Radiosensitivity Testing. in Nuclear technology and radiation protection. 2013;28(2):221-224.
doi:10.2298/NTRP1302221V .

Vujić, Dragana, Petrović, Sandra, Leskovac, Andreja, Joksić, Ivana, Filipović, Jelena G., Valenta-Šobot, Ana, "Accurate Diagnostics of Ataxia-Telangiectasia Cellular Phenotype By Employing in Vitro Lymphocyte Radiosensitivity Testing" in Nuclear technology and radiation protection, 28, no. 2 (2013):221-224,
https://doi.org/10.2298/NTRP1302221V . .

TY  - JOUR
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Valenta-Šobot, Ana
AU  - Joksić, Ivana
AU  - Baltic, M.
AU  - Aleksić, L.
AU  - Joksić, Gordana
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4650
AB  - Background: The aim of this study was to evaluate the effects of two nutraceuticals Amazon Megamin and Immunarc forte in radiorecovery of human lymphocytes after exposure to ionizing radiation in vitro. Material and Methods: The incidences of micronuclei, cell proliferation, apoptosis and lipid peroxidation products were examined in cultured human peripheral blood lymphocytes before and after ionizing radiation in a present of nutraceuticals in vitro. Results: Results showed that Amazon Megamin and Immunarc forte possess antioxidant properties; they act by eliminating the toxic metabolites, scavenging the free radicals and decreasing lipid peroxidation. Conclusion: The obtained results indicated that the studied nutraceuticals can help in prevention of the development of injurious caused by ionizing irradiation and, therefore, they encourage studies on their radioprotective properties.
T2  - European Review for Medical and Pharmacological Sciences
T1  - The radiorecovery potential of nutraceuticals in cellular defense after ionizing radiation in vitro
VL  - 15
IS  - 12
SP  - 1421
EP  - 1427
UR  - https://hdl.handle.net/21.15107/rcub_vinar_4650
ER  - 

@article{
author = "Leskovac, Andreja and Petrović, Sandra and Valenta-Šobot, Ana and Joksić, Ivana and Baltic, M. and Aleksić, L. and Joksić, Gordana",
year = "2011",
abstract = "Background: The aim of this study was to evaluate the effects of two nutraceuticals Amazon Megamin and Immunarc forte in radiorecovery of human lymphocytes after exposure to ionizing radiation in vitro. Material and Methods: The incidences of micronuclei, cell proliferation, apoptosis and lipid peroxidation products were examined in cultured human peripheral blood lymphocytes before and after ionizing radiation in a present of nutraceuticals in vitro. Results: Results showed that Amazon Megamin and Immunarc forte possess antioxidant properties; they act by eliminating the toxic metabolites, scavenging the free radicals and decreasing lipid peroxidation. Conclusion: The obtained results indicated that the studied nutraceuticals can help in prevention of the development of injurious caused by ionizing irradiation and, therefore, they encourage studies on their radioprotective properties.",
journal = "European Review for Medical and Pharmacological Sciences",
title = "The radiorecovery potential of nutraceuticals in cellular defense after ionizing radiation in vitro",
volume = "15",
number = "12",
pages = "1421-1427",
url = "https://hdl.handle.net/21.15107/rcub_vinar_4650"
}

Leskovac, A., Petrović, S., Valenta-Šobot, A., Joksić, I., Baltic, M., Aleksić, L.,& Joksić, G.. (2011). The radiorecovery potential of nutraceuticals in cellular defense after ionizing radiation in vitro. in European Review for Medical and Pharmacological Sciences, 15(12), 1421-1427.
https://hdl.handle.net/21.15107/rcub_vinar_4650

Leskovac A, Petrović S, Valenta-Šobot A, Joksić I, Baltic M, Aleksić L, Joksić G. The radiorecovery potential of nutraceuticals in cellular defense after ionizing radiation in vitro. in European Review for Medical and Pharmacological Sciences. 2011;15(12):1421-1427.
https://hdl.handle.net/21.15107/rcub_vinar_4650 .

Leskovac, Andreja, Petrović, Sandra, Valenta-Šobot, Ana, Joksić, Ivana, Baltic, M., Aleksić, L., Joksić, Gordana, "The radiorecovery potential of nutraceuticals in cellular defense after ionizing radiation in vitro" in European Review for Medical and Pharmacological Sciences, 15, no. 12 (2011):1421-1427,
https://hdl.handle.net/21.15107/rcub_vinar_4650 .

TY  - JOUR
AU  - Leskovac, Andreja
AU  - Vujić, Dragana
AU  - Guć-Šćekić, Marija
AU  - Petrović, Sandra
AU  - Joksić, Ivana
AU  - Slijepcevic, Predrag
AU  - Joksić, Gordana
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4000
AB  - Among patients with bone marrow failure (BMF) syndrome, some are happened to have underlying Fanconi anemia (FA), a genetically heterogeneous disease, which is characterized by progressive pancytopenia and cancer susceptibility. Due to heterogeneous nature of the disease, a single genetic test, as in vitro response to DNA cross-linking agents, usually is not enough to make correct diagnosis. The aim of this study was to evaluate whether measuring repair kinetics of radiation-induced DNA double-strand breaks (DSBs) can distinguish Fanconi anemia from other BMF patients. An early step in repair of DSBs is phosphorylation of the histone H2AX, generating gamma-H2AX histone, which extends over mega base-pair regions of DNA from the break site and is visualised as foci (gamma-H2AX foci) with specific antibodies. The primary fibroblasts, established from FA patients, were exposed to gamma-rays, a dose of 2 Gy (Co-60), incubated for up to 24 hours under repair-permissive conditions, and assayed for the level of gamma-H2AX foci and apoptosis at different recovery times after the treatment. Cell lines originating from FA patients displayed a significant delay in the repair of radiation-induced DNA DSBs relative to non-FA bone marrow failure (non-FA BMF) and control cell lines. The delay is especially evident at recovery time of 24 hours, and is seen as about 8-fold increase of residual gamma-H2AX foci compared to self-state before irradiation. The delay in repair kinetics of FA cells represents the unique feature of FA cellular phenotype, which should be exploited to distinguish FA cellular phenotype.
T2  - Tohoku Journal of Experimental Medicine
T1  - Fanconi Anemia Is Characterized by Delayed Repair Kinetics of DNA Double-Strand Breaks
VL  - 221
IS  - 1
SP  - 69
EP  - 76
DO  - 10.1620/tjem.221.69
ER  - 

@article{
author = "Leskovac, Andreja and Vujić, Dragana and Guć-Šćekić, Marija and Petrović, Sandra and Joksić, Ivana and Slijepcevic, Predrag and Joksić, Gordana",
year = "2010",
abstract = "Among patients with bone marrow failure (BMF) syndrome, some are happened to have underlying Fanconi anemia (FA), a genetically heterogeneous disease, which is characterized by progressive pancytopenia and cancer susceptibility. Due to heterogeneous nature of the disease, a single genetic test, as in vitro response to DNA cross-linking agents, usually is not enough to make correct diagnosis. The aim of this study was to evaluate whether measuring repair kinetics of radiation-induced DNA double-strand breaks (DSBs) can distinguish Fanconi anemia from other BMF patients. An early step in repair of DSBs is phosphorylation of the histone H2AX, generating gamma-H2AX histone, which extends over mega base-pair regions of DNA from the break site and is visualised as foci (gamma-H2AX foci) with specific antibodies. The primary fibroblasts, established from FA patients, were exposed to gamma-rays, a dose of 2 Gy (Co-60), incubated for up to 24 hours under repair-permissive conditions, and assayed for the level of gamma-H2AX foci and apoptosis at different recovery times after the treatment. Cell lines originating from FA patients displayed a significant delay in the repair of radiation-induced DNA DSBs relative to non-FA bone marrow failure (non-FA BMF) and control cell lines. The delay is especially evident at recovery time of 24 hours, and is seen as about 8-fold increase of residual gamma-H2AX foci compared to self-state before irradiation. The delay in repair kinetics of FA cells represents the unique feature of FA cellular phenotype, which should be exploited to distinguish FA cellular phenotype.",
journal = "Tohoku Journal of Experimental Medicine",
title = "Fanconi Anemia Is Characterized by Delayed Repair Kinetics of DNA Double-Strand Breaks",
volume = "221",
number = "1",
pages = "69-76",
doi = "10.1620/tjem.221.69"
}

Leskovac, A., Vujić, D., Guć-Šćekić, M., Petrović, S., Joksić, I., Slijepcevic, P.,& Joksić, G.. (2010). Fanconi Anemia Is Characterized by Delayed Repair Kinetics of DNA Double-Strand Breaks. in Tohoku Journal of Experimental Medicine, 221(1), 69-76.
https://doi.org/10.1620/tjem.221.69

Leskovac A, Vujić D, Guć-Šćekić M, Petrović S, Joksić I, Slijepcevic P, Joksić G. Fanconi Anemia Is Characterized by Delayed Repair Kinetics of DNA Double-Strand Breaks. in Tohoku Journal of Experimental Medicine. 2010;221(1):69-76.
doi:10.1620/tjem.221.69 .

Leskovac, Andreja, Vujić, Dragana, Guć-Šćekić, Marija, Petrović, Sandra, Joksić, Ivana, Slijepcevic, Predrag, Joksić, Gordana, "Fanconi Anemia Is Characterized by Delayed Repair Kinetics of DNA Double-Strand Breaks" in Tohoku Journal of Experimental Medicine, 221, no. 1 (2010):69-76,
https://doi.org/10.1620/tjem.221.69 . .

TY  - JOUR
AU  - Kolarski, Milenko
AU  - Joksić, Gordana
AU  - Beres, Maja
AU  - Krstić, Aleksandar
AU  - Joksić, Ivana
AU  - Dobrojevic, Boris
AU  - Nikic, Slavko
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3625
AB  - We report the first case of Pallister-Killian syndrome diagnosed prenatally in Western Balkan region where one of the ultrasound markers was intrauterine growth restriction. During routine ultrasound control of the pregnancy at 21st gestation week (second pregnancy of the 25 year old woman) symmetrical intrauterine growth restriction (IUGR), short long bones, ventriculomegaly and oligoamnion were noted. Amniotic fluid was examined cytogenetically. Fetal karyotype obtained by GTG banding of amniocytes revealed mosaic female karyotype 46,XX/47,XX,+mar (F-like). C-banding indicated that F-like marker does not belong to F, E or G chromosomal group. Employing targeted FISH with arm-specific probe for chromosome 12, tetrasomy 12p was confirmed. Fetal lymphocytes revealed normal female karyotype. This case showed that i(12p) could be found in pregnancy of young woman, not only in those of advanced age, as usually reported in the literature. This case also showed that intrauterine growth restriction could be one of the ultrasound markers associated with Pallister-Killian syndrome.
T2  - Archives of Gynecology and Obstetrics
T1  - Prenatal diagnosis of Pallister-Killian syndrome in young woman: ultrasound indicators and confirmation by FISH
VL  - 279
IS  - 3
SP  - 377
EP  - 379
DO  - 10.1007/s00404-008-0704-6
ER  - 

@article{
author = "Kolarski, Milenko and Joksić, Gordana and Beres, Maja and Krstić, Aleksandar and Joksić, Ivana and Dobrojevic, Boris and Nikic, Slavko",
year = "2009",
abstract = "We report the first case of Pallister-Killian syndrome diagnosed prenatally in Western Balkan region where one of the ultrasound markers was intrauterine growth restriction. During routine ultrasound control of the pregnancy at 21st gestation week (second pregnancy of the 25 year old woman) symmetrical intrauterine growth restriction (IUGR), short long bones, ventriculomegaly and oligoamnion were noted. Amniotic fluid was examined cytogenetically. Fetal karyotype obtained by GTG banding of amniocytes revealed mosaic female karyotype 46,XX/47,XX,+mar (F-like). C-banding indicated that F-like marker does not belong to F, E or G chromosomal group. Employing targeted FISH with arm-specific probe for chromosome 12, tetrasomy 12p was confirmed. Fetal lymphocytes revealed normal female karyotype. This case showed that i(12p) could be found in pregnancy of young woman, not only in those of advanced age, as usually reported in the literature. This case also showed that intrauterine growth restriction could be one of the ultrasound markers associated with Pallister-Killian syndrome.",
journal = "Archives of Gynecology and Obstetrics",
title = "Prenatal diagnosis of Pallister-Killian syndrome in young woman: ultrasound indicators and confirmation by FISH",
volume = "279",
number = "3",
pages = "377-379",
doi = "10.1007/s00404-008-0704-6"
}

Kolarski, M., Joksić, G., Beres, M., Krstić, A., Joksić, I., Dobrojevic, B.,& Nikic, S.. (2009). Prenatal diagnosis of Pallister-Killian syndrome in young woman: ultrasound indicators and confirmation by FISH. in Archives of Gynecology and Obstetrics, 279(3), 377-379.
https://doi.org/10.1007/s00404-008-0704-6

Kolarski M, Joksić G, Beres M, Krstić A, Joksić I, Dobrojevic B, Nikic S. Prenatal diagnosis of Pallister-Killian syndrome in young woman: ultrasound indicators and confirmation by FISH. in Archives of Gynecology and Obstetrics. 2009;279(3):377-379.
doi:10.1007/s00404-008-0704-6 .

Kolarski, Milenko, Joksić, Gordana, Beres, Maja, Krstić, Aleksandar, Joksić, Ivana, Dobrojevic, Boris, Nikic, Slavko, "Prenatal diagnosis of Pallister-Killian syndrome in young woman: ultrasound indicators and confirmation by FISH" in Archives of Gynecology and Obstetrics, 279, no. 3 (2009):377-379,
https://doi.org/10.1007/s00404-008-0704-6 . .

TY  - JOUR
AU  - Joksić, Gordana
AU  - Petrović, Sandra
AU  - Joksić, Ivana
AU  - Leskovac, Andreja
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3728
AB  - The aim of this study was to investigate radioprotective properties of Echinacea purpurea tablets in vivo. We analysed lymphocyte chromosome aberrations (CA), micronuclei (MN), apoptosis of leukocytes and haematological parameters in a group of radiation workers who were identified as carrying dicentric chromosomes in their lymphocytes. All radiation workers were taking two 275 mg Echinacea tablets b.i.d., according to a pharmacists recommendation. All parameters were analysed before and after the two-week treatment. At the end of the treatment lymphocyte CA frequency dropped significantly, and the number of apoptotic cells increased. The inverse lymphocyte-to-granulocyte ratio at the beginning of the study changed to normal at its end. In conclusion, biological effects observed after administration of Echinacea purpurea preparation suggest that it may be beneficial for the prevention of adverse health effects in workers exposed to ionising radiation.
T2  - Arhiv za higijenu rada i toksikologiju
T1  - Biological Effects of Echinacea Purpurea on Human Blood Cells
VL  - 60
IS  - 2
SP  - 165
EP  - 172
DO  - 10.2478/10004-1254-60-2009-1920
ER  - 

@article{
author = "Joksić, Gordana and Petrović, Sandra and Joksić, Ivana and Leskovac, Andreja",
year = "2009",
abstract = "The aim of this study was to investigate radioprotective properties of Echinacea purpurea tablets in vivo. We analysed lymphocyte chromosome aberrations (CA), micronuclei (MN), apoptosis of leukocytes and haematological parameters in a group of radiation workers who were identified as carrying dicentric chromosomes in their lymphocytes. All radiation workers were taking two 275 mg Echinacea tablets b.i.d., according to a pharmacists recommendation. All parameters were analysed before and after the two-week treatment. At the end of the treatment lymphocyte CA frequency dropped significantly, and the number of apoptotic cells increased. The inverse lymphocyte-to-granulocyte ratio at the beginning of the study changed to normal at its end. In conclusion, biological effects observed after administration of Echinacea purpurea preparation suggest that it may be beneficial for the prevention of adverse health effects in workers exposed to ionising radiation.",
journal = "Arhiv za higijenu rada i toksikologiju",
title = "Biological Effects of Echinacea Purpurea on Human Blood Cells",
volume = "60",
number = "2",
pages = "165-172",
doi = "10.2478/10004-1254-60-2009-1920"
}

Joksić, G., Petrović, S., Joksić, I.,& Leskovac, A.. (2009). Biological Effects of Echinacea Purpurea on Human Blood Cells. in Arhiv za higijenu rada i toksikologiju, 60(2), 165-172.
https://doi.org/10.2478/10004-1254-60-2009-1920

Joksić G, Petrović S, Joksić I, Leskovac A. Biological Effects of Echinacea Purpurea on Human Blood Cells. in Arhiv za higijenu rada i toksikologiju. 2009;60(2):165-172.
doi:10.2478/10004-1254-60-2009-1920 .

Joksić, Gordana, Petrović, Sandra, Joksić, Ivana, Leskovac, Andreja, "Biological Effects of Echinacea Purpurea on Human Blood Cells" in Arhiv za higijenu rada i toksikologiju, 60, no. 2 (2009):165-172,
https://doi.org/10.2478/10004-1254-60-2009-1920 . .

TY  - JOUR
AU  - Joksić, Ivana
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Joksić, Gordana
PY  - 2006
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3047
AB  - JOKSIC I., LESKOVAC, A., PETROVIC, S. and JOKSI, G. Vitamin B12 Reduces Ribavirin-Induced Genotoxicity in Phytohemaglutinin-Stimulated Human Lymphocytes. Toboku J. Exp. Med., 2006, 209 (4), 347-354 - Ribavirin, an N-glycosyl nucleoside (1-,6-D-ribofuranosyl-1, 2, 4-triazole-3 carboxamide), is a synthetic purine nucleoside analogue with a broad spectrum of antiviral activity, however, its high toxicity poses a major disadvantage of its use as a therapeutic. Various studies have shown that vitamin B 12 plays a significant role in maintaining the stability of the human genome. We therefore investigated the potential beneficial effect of vitamin B 12 in reducing ribavirin-induced genotoxicity. To test this, we used the cytokinesis-block micronucleus (CBMN) assay. Human blood cells were treated in vitro with increasing doses of ribavirin (0.05, 0.17, 0.32, 0.47 and 0.65,umol/ml) for three different periods of time (2, 4 and 17 hrs). Duplicate cultures were supplemented with 50 mu l of vitamin B 12 during the drug treatment (final concentration of 13.5 mu g/ml). Micronuclei formation and cell proliferation potential were then scored in both sets of samples and the corresponding controls. The results showed that supplementation with vitamin B 12 lowered the frequency of micronuclei (Z = 2.02, p LT 0.04) and recovered the proliferation potential of the treated cells for each treatment period, except for the conditions with the highest concentration of ribavirin and the shortest time. These observations underscore the unique beneficial effects of vitamin B12 in reducing genotoxicity, particularly by recovering the proliferation potential of treated cells, as demonstrated by the decrease in mononucleated cells and enhancement of binucleated and polynucleated cells. The mechanism by which vitamin B 12 reduces ribavirin-induced genotoxicity is related to de novo synthesis of nucleotides, and is worthy of further investigation. ribavirin; reduced genotoxicity; vitamin B 12; human lymphocytes (c) 2006 Tohoku University Medical Press.
T2  - Tohoku Journal of Experimental Medicine
T1  - Vitamin B12 reduces ribavirin-induced genotoxicity in phytohemaglutinin-stimulated human lymphocytes
VL  - 209
IS  - 4
SP  - 347
EP  - 354
DO  - 10.1620/tjem.209.347
ER  - 

@article{
author = "Joksić, Ivana and Leskovac, Andreja and Petrović, Sandra and Joksić, Gordana",
year = "2006",
abstract = "JOKSIC I., LESKOVAC, A., PETROVIC, S. and JOKSI, G. Vitamin B12 Reduces Ribavirin-Induced Genotoxicity in Phytohemaglutinin-Stimulated Human Lymphocytes. Toboku J. Exp. Med., 2006, 209 (4), 347-354 - Ribavirin, an N-glycosyl nucleoside (1-,6-D-ribofuranosyl-1, 2, 4-triazole-3 carboxamide), is a synthetic purine nucleoside analogue with a broad spectrum of antiviral activity, however, its high toxicity poses a major disadvantage of its use as a therapeutic. Various studies have shown that vitamin B 12 plays a significant role in maintaining the stability of the human genome. We therefore investigated the potential beneficial effect of vitamin B 12 in reducing ribavirin-induced genotoxicity. To test this, we used the cytokinesis-block micronucleus (CBMN) assay. Human blood cells were treated in vitro with increasing doses of ribavirin (0.05, 0.17, 0.32, 0.47 and 0.65,umol/ml) for three different periods of time (2, 4 and 17 hrs). Duplicate cultures were supplemented with 50 mu l of vitamin B 12 during the drug treatment (final concentration of 13.5 mu g/ml). Micronuclei formation and cell proliferation potential were then scored in both sets of samples and the corresponding controls. The results showed that supplementation with vitamin B 12 lowered the frequency of micronuclei (Z = 2.02, p LT 0.04) and recovered the proliferation potential of the treated cells for each treatment period, except for the conditions with the highest concentration of ribavirin and the shortest time. These observations underscore the unique beneficial effects of vitamin B12 in reducing genotoxicity, particularly by recovering the proliferation potential of treated cells, as demonstrated by the decrease in mononucleated cells and enhancement of binucleated and polynucleated cells. The mechanism by which vitamin B 12 reduces ribavirin-induced genotoxicity is related to de novo synthesis of nucleotides, and is worthy of further investigation. ribavirin; reduced genotoxicity; vitamin B 12; human lymphocytes (c) 2006 Tohoku University Medical Press.",
journal = "Tohoku Journal of Experimental Medicine",
title = "Vitamin B12 reduces ribavirin-induced genotoxicity in phytohemaglutinin-stimulated human lymphocytes",
volume = "209",
number = "4",
pages = "347-354",
doi = "10.1620/tjem.209.347"
}

Joksić, I., Leskovac, A., Petrović, S.,& Joksić, G.. (2006). Vitamin B12 reduces ribavirin-induced genotoxicity in phytohemaglutinin-stimulated human lymphocytes. in Tohoku Journal of Experimental Medicine, 209(4), 347-354.
https://doi.org/10.1620/tjem.209.347

Joksić I, Leskovac A, Petrović S, Joksić G. Vitamin B12 reduces ribavirin-induced genotoxicity in phytohemaglutinin-stimulated human lymphocytes. in Tohoku Journal of Experimental Medicine. 2006;209(4):347-354.
doi:10.1620/tjem.209.347 .

Joksić, Ivana, Leskovac, Andreja, Petrović, Sandra, Joksić, Gordana, "Vitamin B12 reduces ribavirin-induced genotoxicity in phytohemaglutinin-stimulated human lymphocytes" in Tohoku Journal of Experimental Medicine, 209, no. 4 (2006):347-354,
https://doi.org/10.1620/tjem.209.347 . .