TY  - CONF
AU  - Cvetković, S
AU  - Nastasijević, Branislav J.
AU  - Mitić-Ćulafić, D
AU  - Đukanović, S
AU  - Nikolić, B
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10057
AB  - Lipid oxidation that occurs in different types of food can cause alterations in nutritional qualities, flavour, texture and shelf life of foods. Furthermore, high temperature cooking of protein-rich food can lead to formation of heterocyclic aromatic amines capable of compromising the integrity of DNA molecules. To reduce these harmful effects, research has been focused on investigating plants as a source of potential natural food additives and preservatives. Thus, the aim of this study was to estimate antioxidant and antigenotoxic activities of 50% ethanolic-aqueous root and leaf extracts of the medicinal plant, Gentiana lutea . Antioxidative effect was investigated using the DPPH assay, while antigenotoxicity against the mutagen 2-amino-1-methyl-6-phenylimidazo[4,5- b ]pyridine (PhIP) was determined using Salmonella Typhimurium TA 1535 in the SOS/ umuC assay. Leaf extract showed high antioxidative effect with the ability to neutralize up to 87% of free radicals at 400 µg mL -1 . Antigenotoxicity testing revealed that both extracts exhibited remarkable genoprotective activity against PhIP-induced DNA damage, with the highest inhibition levels being 70% and 85% for root and leaf extracts, respectively. Results obtained are encouraging and suggest further research of G. lutea extracts as potential food preservatives and additives in improving food quality and human health.
C3  - IOP Conference Series: Earth and Environmental Science
T1  - Antioxidative properties and antigenotoxic potential of Gentiana lutea extracts against the heterocyclic aromatic amine 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine, PhIP
VL  - 854
IS  - 1
SP  - 012018
DO  - 10.1088/1755-1315/854/1/012018
ER  - 

@conference{
author = "Cvetković, S and Nastasijević, Branislav J. and Mitić-Ćulafić, D and Đukanović, S and Nikolić, B",
year = "2021",
abstract = "Lipid oxidation that occurs in different types of food can cause alterations in nutritional qualities, flavour, texture and shelf life of foods. Furthermore, high temperature cooking of protein-rich food can lead to formation of heterocyclic aromatic amines capable of compromising the integrity of DNA molecules. To reduce these harmful effects, research has been focused on investigating plants as a source of potential natural food additives and preservatives. Thus, the aim of this study was to estimate antioxidant and antigenotoxic activities of 50% ethanolic-aqueous root and leaf extracts of the medicinal plant, Gentiana lutea . Antioxidative effect was investigated using the DPPH assay, while antigenotoxicity against the mutagen 2-amino-1-methyl-6-phenylimidazo[4,5- b ]pyridine (PhIP) was determined using Salmonella Typhimurium TA 1535 in the SOS/ umuC assay. Leaf extract showed high antioxidative effect with the ability to neutralize up to 87% of free radicals at 400 µg mL -1 . Antigenotoxicity testing revealed that both extracts exhibited remarkable genoprotective activity against PhIP-induced DNA damage, with the highest inhibition levels being 70% and 85% for root and leaf extracts, respectively. Results obtained are encouraging and suggest further research of G. lutea extracts as potential food preservatives and additives in improving food quality and human health.",
journal = "IOP Conference Series: Earth and Environmental Science",
title = "Antioxidative properties and antigenotoxic potential of Gentiana lutea extracts against the heterocyclic aromatic amine 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine, PhIP",
volume = "854",
number = "1",
pages = "012018",
doi = "10.1088/1755-1315/854/1/012018"
}

Cvetković, S., Nastasijević, B. J., Mitić-Ćulafić, D., Đukanović, S.,& Nikolić, B.. (2021). Antioxidative properties and antigenotoxic potential of Gentiana lutea extracts against the heterocyclic aromatic amine 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine, PhIP. in IOP Conference Series: Earth and Environmental Science, 854(1), 012018.
https://doi.org/10.1088/1755-1315/854/1/012018

Cvetković S, Nastasijević BJ, Mitić-Ćulafić D, Đukanović S, Nikolić B. Antioxidative properties and antigenotoxic potential of Gentiana lutea extracts against the heterocyclic aromatic amine 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine, PhIP. in IOP Conference Series: Earth and Environmental Science. 2021;854(1):012018.
doi:10.1088/1755-1315/854/1/012018 .

Cvetković, S, Nastasijević, Branislav J., Mitić-Ćulafić, D, Đukanović, S, Nikolić, B, "Antioxidative properties and antigenotoxic potential of Gentiana lutea extracts against the heterocyclic aromatic amine 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine, PhIP" in IOP Conference Series: Earth and Environmental Science, 854, no. 1 (2021):012018,
https://doi.org/10.1088/1755-1315/854/1/012018 . .

TY  - JOUR
AU  - Pejić, Snežana
AU  - Todorović, Ana
AU  - Stojiljković, Vesna
AU  - Cvetković, Dragana
AU  - Lucic, Nenad
AU  - Radojicic, Ratko M.
AU  - Saičić, Zorica S.
AU  - Pajović, Snežana B.
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3520
AB  - Epidemiological and experimental data point to involvement of oxygen derived radicals in the pathogenesis of gynecological disorders, as well as in cancer development. The objective of the present study was to examine changes ill activities and levels of copper/zinc superoxide dismutase (CuZnSOD) and lipid hydroperoxides (LOOH) in blood and endometrial tissue of patients diagnosed With uterine myoma, endometrial polypus, hyperplasia simplex, hyperplasia complex and adenocarcinoma endometrii. The results of our study have shown decreased SOD activities and unchanged SOD protein level in blood of all examined patients in comparison to healthy Subjects. Decrease of both SOD activity and level was Found in endometrium of patients with hyperplasia simplex, hyperplasia complex and adenocarcinoma in comparison to women with polypus or myoma. LOOH level was elevated in both tissues of patients with hyperplasia or adenocarcinoma in comparison to healthy Subjects or patients with benign diagnosis. Our findings suggest that the decrease in SOD activity and level, as well as the increase in LOOH level, in patients with gynecological disorders. render these patients more Susceptible to oxidative damage caused by reactive oxygen species (ROS). An imbalance in ROS formation and SOD level may be important in the pathogenesis and/or perpetuation of tissue damage in gynecological patients. Since evidence suggests that SOD may be a therapy target for cancer treatment, our findings provide a basis for further research and options for clinical applications.
T2  - Anais de Academia Brasileira de Ciencias
T1  - Superoxide dismutase and lipid hydroperoxides in blood and endometrial tissue of patients with benign, hyperplastic and malignant endometrium
VL  - 80
IS  - 3
SP  - 515
EP  - 522
DO  - 10.1590/S0001-37652008000300011
ER  - 

@article{
author = "Pejić, Snežana and Todorović, Ana and Stojiljković, Vesna and Cvetković, Dragana and Lucic, Nenad and Radojicic, Ratko M. and Saičić, Zorica S. and Pajović, Snežana B.",
year = "2008",
abstract = "Epidemiological and experimental data point to involvement of oxygen derived radicals in the pathogenesis of gynecological disorders, as well as in cancer development. The objective of the present study was to examine changes ill activities and levels of copper/zinc superoxide dismutase (CuZnSOD) and lipid hydroperoxides (LOOH) in blood and endometrial tissue of patients diagnosed With uterine myoma, endometrial polypus, hyperplasia simplex, hyperplasia complex and adenocarcinoma endometrii. The results of our study have shown decreased SOD activities and unchanged SOD protein level in blood of all examined patients in comparison to healthy Subjects. Decrease of both SOD activity and level was Found in endometrium of patients with hyperplasia simplex, hyperplasia complex and adenocarcinoma in comparison to women with polypus or myoma. LOOH level was elevated in both tissues of patients with hyperplasia or adenocarcinoma in comparison to healthy Subjects or patients with benign diagnosis. Our findings suggest that the decrease in SOD activity and level, as well as the increase in LOOH level, in patients with gynecological disorders. render these patients more Susceptible to oxidative damage caused by reactive oxygen species (ROS). An imbalance in ROS formation and SOD level may be important in the pathogenesis and/or perpetuation of tissue damage in gynecological patients. Since evidence suggests that SOD may be a therapy target for cancer treatment, our findings provide a basis for further research and options for clinical applications.",
journal = "Anais de Academia Brasileira de Ciencias",
title = "Superoxide dismutase and lipid hydroperoxides in blood and endometrial tissue of patients with benign, hyperplastic and malignant endometrium",
volume = "80",
number = "3",
pages = "515-522",
doi = "10.1590/S0001-37652008000300011"
}

Pejić, S., Todorović, A., Stojiljković, V., Cvetković, D., Lucic, N., Radojicic, R. M., Saičić, Z. S.,& Pajović, S. B.. (2008). Superoxide dismutase and lipid hydroperoxides in blood and endometrial tissue of patients with benign, hyperplastic and malignant endometrium. in Anais de Academia Brasileira de Ciencias, 80(3), 515-522.
https://doi.org/10.1590/S0001-37652008000300011

Pejić S, Todorović A, Stojiljković V, Cvetković D, Lucic N, Radojicic RM, Saičić ZS, Pajović SB. Superoxide dismutase and lipid hydroperoxides in blood and endometrial tissue of patients with benign, hyperplastic and malignant endometrium. in Anais de Academia Brasileira de Ciencias. 2008;80(3):515-522.
doi:10.1590/S0001-37652008000300011 .

Pejić, Snežana, Todorović, Ana, Stojiljković, Vesna, Cvetković, Dragana, Lucic, Nenad, Radojicic, Ratko M., Saičić, Zorica S., Pajović, Snežana B., "Superoxide dismutase and lipid hydroperoxides in blood and endometrial tissue of patients with benign, hyperplastic and malignant endometrium" in Anais de Academia Brasileira de Ciencias, 80, no. 3 (2008):515-522,
https://doi.org/10.1590/S0001-37652008000300011 . .