TY  - JOUR
AU  - Zmejkoski, Danica
AU  - Marković, Zoran M.
AU  - Zdravković, Nemanja M.
AU  - Trišić, Dijana
AU  - Budimir, Milica
AU  - Kuzman, Sanja
AU  - Kozyrovska, Natalia O.
AU  - Orlovska, Iryna V.
AU  - Bugárová, Nikol
AU  - Petrović, Đorđe
AU  - Kováčová, Mária
AU  - Kleinová, Angela
AU  - Špitalský, Zdeno
AU  - Pavlović, Vladimir B.
AU  - Todorović-Marković, Biljana
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9143
AB  - Therapy of bacterial urinary tract infections (UTIs) and catheter associated urinary tract infections (CAUTIs) is still a great challenge because of the resistance of bacteria to nowadays used antibiotics and encrustation of catheters. Bacterial cellulose (BC) as a biocompatible material with a high porosity allows incorporation of different materials in its three dimensional network structure. In this work a low molecular weight chitosan (Chi) polymer is incorporated in BC with different concentrations. Different characterization techniques are used to investigate structural and optical properties of these composites. Radical scavenging activity test shows moderate antioxidant activity of these biocompatible composites whereasin vitrorelease test shows that 13.3% of chitosan is released after 72 h. Antibacterial testing of BC-Chi composites conducted on Gram-positive and Gram-negative bacteria causing UTIs and CAUTIs (Escherichia coli,Pseudomonas aeruginosa,Klebsiella pneumoniae) and encrustation (Proteus mirabilis) show bactericidal effect. The morphology analysis of bacteria after the application of BC-Chi shows that they are flattened with a rough surface, with a tendency to agglomerate and with decreased length and width. All obtained results show that BC-Chi composites might be considered as potential biomedical agents in treatment of UTIs and CAUTIs and as a urinary catheter coating in encrustation prevention.
T2  - RSC Advances
T1  - Bactericidal and antioxidant bacterial cellulose hydrogels doped with chitosan as potential urinary tract infection biomedical agent
VL  - 11
IS  - 15
SP  - 8559
EP  - 8568
DO  - 10.1039/D0RA10782D
ER  - 

@article{
author = "Zmejkoski, Danica and Marković, Zoran M. and Zdravković, Nemanja M. and Trišić, Dijana and Budimir, Milica and Kuzman, Sanja and Kozyrovska, Natalia O. and Orlovska, Iryna V. and Bugárová, Nikol and Petrović, Đorđe and Kováčová, Mária and Kleinová, Angela and Špitalský, Zdeno and Pavlović, Vladimir B. and Todorović-Marković, Biljana",
year = "2021",
abstract = "Therapy of bacterial urinary tract infections (UTIs) and catheter associated urinary tract infections (CAUTIs) is still a great challenge because of the resistance of bacteria to nowadays used antibiotics and encrustation of catheters. Bacterial cellulose (BC) as a biocompatible material with a high porosity allows incorporation of different materials in its three dimensional network structure. In this work a low molecular weight chitosan (Chi) polymer is incorporated in BC with different concentrations. Different characterization techniques are used to investigate structural and optical properties of these composites. Radical scavenging activity test shows moderate antioxidant activity of these biocompatible composites whereasin vitrorelease test shows that 13.3% of chitosan is released after 72 h. Antibacterial testing of BC-Chi composites conducted on Gram-positive and Gram-negative bacteria causing UTIs and CAUTIs (Escherichia coli,Pseudomonas aeruginosa,Klebsiella pneumoniae) and encrustation (Proteus mirabilis) show bactericidal effect. The morphology analysis of bacteria after the application of BC-Chi shows that they are flattened with a rough surface, with a tendency to agglomerate and with decreased length and width. All obtained results show that BC-Chi composites might be considered as potential biomedical agents in treatment of UTIs and CAUTIs and as a urinary catheter coating in encrustation prevention.",
journal = "RSC Advances",
title = "Bactericidal and antioxidant bacterial cellulose hydrogels doped with chitosan as potential urinary tract infection biomedical agent",
volume = "11",
number = "15",
pages = "8559-8568",
doi = "10.1039/D0RA10782D"
}

Zmejkoski, D., Marković, Z. M., Zdravković, N. M., Trišić, D., Budimir, M., Kuzman, S., Kozyrovska, N. O., Orlovska, I. V., Bugárová, N., Petrović, Đ., Kováčová, M., Kleinová, A., Špitalský, Z., Pavlović, V. B.,& Todorović-Marković, B.. (2021). Bactericidal and antioxidant bacterial cellulose hydrogels doped with chitosan as potential urinary tract infection biomedical agent. in RSC Advances, 11(15), 8559-8568.
https://doi.org/10.1039/D0RA10782D

Zmejkoski D, Marković ZM, Zdravković NM, Trišić D, Budimir M, Kuzman S, Kozyrovska NO, Orlovska IV, Bugárová N, Petrović Đ, Kováčová M, Kleinová A, Špitalský Z, Pavlović VB, Todorović-Marković B. Bactericidal and antioxidant bacterial cellulose hydrogels doped with chitosan as potential urinary tract infection biomedical agent. in RSC Advances. 2021;11(15):8559-8568.
doi:10.1039/D0RA10782D .

Zmejkoski, Danica, Marković, Zoran M., Zdravković, Nemanja M., Trišić, Dijana, Budimir, Milica, Kuzman, Sanja, Kozyrovska, Natalia O., Orlovska, Iryna V., Bugárová, Nikol, Petrović, Đorđe, Kováčová, Mária, Kleinová, Angela, Špitalský, Zdeno, Pavlović, Vladimir B., Todorović-Marković, Biljana, "Bactericidal and antioxidant bacterial cellulose hydrogels doped with chitosan as potential urinary tract infection biomedical agent" in RSC Advances, 11, no. 15 (2021):8559-8568,
https://doi.org/10.1039/D0RA10782D . .

TY  - JOUR
AU  - Orlovska, Iryna
AU  - Podolich, Olga
AU  - Kukharenko, Olga
AU  - Zaets, Iryna
AU  - Reva, Oleg
AU  - Khirunenko, Ludmila
AU  - Zmejkoski, Danica
AU  - Rogalsky, Sergiy
AU  - Barh, Debmalya
AU  - Tiwari, Sandeep
AU  - Kumavath, Ranjith
AU  - Góes-Neto, Aristóteles
AU  - Azevedo, Vasco
AU  - Brenig, Bertram
AU  - Ghosh, Preetam
AU  - de Vera, Jean-Pierre
AU  - Kozyrovska, Natalia
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9849
AB  - Cellulose is a widespread macromolecule in terrestrial environments and a major architectural component of microbial biofilm. Therefore, cellulose might be considered a biosignature that indicates the presence of microbial life. We present, for the first time, characteristics of bacterial cellulose after long-term spaceflight and exposure to simuled Mars-like stressors. The pristine cellulose-based pellicle membranes from a kombucha microbial community (KMC) were exposed outside the International Space Station, and after their return to Earth, the samples were reactivated and cultured for 2.5 years to discern whether the KMC could be restored. Analyses of cellulose polymer integrity and mechanical properties of cellulose-based pellicle films, as well as the cellulose biosynthesis-related genes' structure and expression, were performed. We observed that (i) the cellulose polymer integrity was not significantly changed under Mars-like conditions; (ii) de novo cellulose production was 1.5 times decreased in exposed KMC samples; (iii) the dry cellulose yield from the reisolated Komagataeibacter oboediens was 1.7 times lower than by wild type; (iv) there was no significant change in mechanical properties of the de novo synthesized cellulose-based pellicles produced by the exposed KMCs and K. oboediens; and (v) the gene, encoding biosynthesis of cellulose (bcsA) of the K. oboediens, was downregulated, and no topological change or mutation was observed in any of the bcs operon genes, indicating that the decreased cellulose production by the space-exposed samples was probably due to epigenetic regulation. Our results suggest that the cellulose-based pellicle could be a good material with which to protect microbial communities during space journeys, and the cellulose produced by KMC members could be suitable in the fabrication of consumer goods for extraterrestrial locations.
T2  - Astrobiology
T1  - Bacterial Cellulose Retains Robustness but Its Synthesis Declines After Exposure to a Mars-like Environment Simulated Outside the International Space Station
VL  - 21
IS  - 6
SP  - 706
EP  - 717
DO  - 10.1089/ast.2020.2332
ER  - 

@article{
author = "Orlovska, Iryna and Podolich, Olga and Kukharenko, Olga and Zaets, Iryna and Reva, Oleg and Khirunenko, Ludmila and Zmejkoski, Danica and Rogalsky, Sergiy and Barh, Debmalya and Tiwari, Sandeep and Kumavath, Ranjith and Góes-Neto, Aristóteles and Azevedo, Vasco and Brenig, Bertram and Ghosh, Preetam and de Vera, Jean-Pierre and Kozyrovska, Natalia",
year = "2021",
abstract = "Cellulose is a widespread macromolecule in terrestrial environments and a major architectural component of microbial biofilm. Therefore, cellulose might be considered a biosignature that indicates the presence of microbial life. We present, for the first time, characteristics of bacterial cellulose after long-term spaceflight and exposure to simuled Mars-like stressors. The pristine cellulose-based pellicle membranes from a kombucha microbial community (KMC) were exposed outside the International Space Station, and after their return to Earth, the samples were reactivated and cultured for 2.5 years to discern whether the KMC could be restored. Analyses of cellulose polymer integrity and mechanical properties of cellulose-based pellicle films, as well as the cellulose biosynthesis-related genes' structure and expression, were performed. We observed that (i) the cellulose polymer integrity was not significantly changed under Mars-like conditions; (ii) de novo cellulose production was 1.5 times decreased in exposed KMC samples; (iii) the dry cellulose yield from the reisolated Komagataeibacter oboediens was 1.7 times lower than by wild type; (iv) there was no significant change in mechanical properties of the de novo synthesized cellulose-based pellicles produced by the exposed KMCs and K. oboediens; and (v) the gene, encoding biosynthesis of cellulose (bcsA) of the K. oboediens, was downregulated, and no topological change or mutation was observed in any of the bcs operon genes, indicating that the decreased cellulose production by the space-exposed samples was probably due to epigenetic regulation. Our results suggest that the cellulose-based pellicle could be a good material with which to protect microbial communities during space journeys, and the cellulose produced by KMC members could be suitable in the fabrication of consumer goods for extraterrestrial locations.",
journal = "Astrobiology",
title = "Bacterial Cellulose Retains Robustness but Its Synthesis Declines After Exposure to a Mars-like Environment Simulated Outside the International Space Station",
volume = "21",
number = "6",
pages = "706-717",
doi = "10.1089/ast.2020.2332"
}

Orlovska, I., Podolich, O., Kukharenko, O., Zaets, I., Reva, O., Khirunenko, L., Zmejkoski, D., Rogalsky, S., Barh, D., Tiwari, S., Kumavath, R., Góes-Neto, A., Azevedo, V., Brenig, B., Ghosh, P., de Vera, J.,& Kozyrovska, N.. (2021). Bacterial Cellulose Retains Robustness but Its Synthesis Declines After Exposure to a Mars-like Environment Simulated Outside the International Space Station. in Astrobiology, 21(6), 706-717.
https://doi.org/10.1089/ast.2020.2332

Orlovska I, Podolich O, Kukharenko O, Zaets I, Reva O, Khirunenko L, Zmejkoski D, Rogalsky S, Barh D, Tiwari S, Kumavath R, Góes-Neto A, Azevedo V, Brenig B, Ghosh P, de Vera J, Kozyrovska N. Bacterial Cellulose Retains Robustness but Its Synthesis Declines After Exposure to a Mars-like Environment Simulated Outside the International Space Station. in Astrobiology. 2021;21(6):706-717.
doi:10.1089/ast.2020.2332 .

Orlovska, Iryna, Podolich, Olga, Kukharenko, Olga, Zaets, Iryna, Reva, Oleg, Khirunenko, Ludmila, Zmejkoski, Danica, Rogalsky, Sergiy, Barh, Debmalya, Tiwari, Sandeep, Kumavath, Ranjith, Góes-Neto, Aristóteles, Azevedo, Vasco, Brenig, Bertram, Ghosh, Preetam, de Vera, Jean-Pierre, Kozyrovska, Natalia, "Bacterial Cellulose Retains Robustness but Its Synthesis Declines After Exposure to a Mars-like Environment Simulated Outside the International Space Station" in Astrobiology, 21, no. 6 (2021):706-717,
https://doi.org/10.1089/ast.2020.2332 . .

TY  - CONF
AU  - Orlovska, Iryna
AU  - Zmejkoski, Danica
AU  - Khirunenko, L.
AU  - Kozyrovska, N.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13008
AB  - Biofilm-forming multi-drug resistant pathogens may account for 10% of all hospital acquired infections and can occur in burns, post-surgical wounds, pressure injuries and diabetic foot wounds. Hydrogels – natural polymers or composite materials – represent both a bandage and a matrix for therapeutics in a wound healing. Novel composite based on bacterial cellulose and synthetic analog of lignin - DHP (dehydrogenate polymer) has been designed with the aim to suppress pathogenic agents in incurable wounds. The FT-IR spectra of the BC/DHP membrane established an appearance of characteristic for DHP absorption bands in the composite in the 1600–1510 cm−1 spectral range. A clear position of these bands in BC/DHP showed that DHP had been incorporated into the BC polymeric matrix. In addition, disappearance of the bands at the 3630–3650 cm−1 spectral range assigned to the cellulose free OH vibration, strengthening the bands assigned to intermolecular interactions and an appearance of a new band at 3293 cm−1 could serve as a proof of the interaction of BC molecules and DHP. Micrographs obtained from a scanning electron microscopy proved the dose-dependent interaction of DHP with BC, which resulted in a decrease of the pore number in the cellulose membrane. The antimicrobial activity of composites BC/DHP were tested the in vitro experiments, using Pseudomonas aeruginosa (УМВ-100) (MTT-test) and showed that BC/DHP inhibited P. aeruginosa. BC/DHP composite will be promising hydrogel for a healing of infected incurable wounds.
C3  - Integrative Biology & Medicine : Program and Abstracts
T1  - A composite of bacterial cellulose and lignin for a healing of incurable wounds
SP  - 53
EP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_vinar_13008
ER  - 

@conference{
author = "Orlovska, Iryna and Zmejkoski, Danica and Khirunenko, L. and Kozyrovska, N.",
year = "2017",
abstract = "Biofilm-forming multi-drug resistant pathogens may account for 10% of all hospital acquired infections and can occur in burns, post-surgical wounds, pressure injuries and diabetic foot wounds. Hydrogels – natural polymers or composite materials – represent both a bandage and a matrix for therapeutics in a wound healing. Novel composite based on bacterial cellulose and synthetic analog of lignin - DHP (dehydrogenate polymer) has been designed with the aim to suppress pathogenic agents in incurable wounds. The FT-IR spectra of the BC/DHP membrane established an appearance of characteristic for DHP absorption bands in the composite in the 1600–1510 cm−1 spectral range. A clear position of these bands in BC/DHP showed that DHP had been incorporated into the BC polymeric matrix. In addition, disappearance of the bands at the 3630–3650 cm−1 spectral range assigned to the cellulose free OH vibration, strengthening the bands assigned to intermolecular interactions and an appearance of a new band at 3293 cm−1 could serve as a proof of the interaction of BC molecules and DHP. Micrographs obtained from a scanning electron microscopy proved the dose-dependent interaction of DHP with BC, which resulted in a decrease of the pore number in the cellulose membrane. The antimicrobial activity of composites BC/DHP were tested the in vitro experiments, using Pseudomonas aeruginosa (УМВ-100) (MTT-test) and showed that BC/DHP inhibited P. aeruginosa. BC/DHP composite will be promising hydrogel for a healing of infected incurable wounds.",
journal = "Integrative Biology & Medicine : Program and Abstracts",
title = "A composite of bacterial cellulose and lignin for a healing of incurable wounds",
pages = "53-53",
url = "https://hdl.handle.net/21.15107/rcub_vinar_13008"
}

Orlovska, I., Zmejkoski, D., Khirunenko, L.,& Kozyrovska, N.. (2017). A composite of bacterial cellulose and lignin for a healing of incurable wounds. in Integrative Biology & Medicine : Program and Abstracts, 53-53.
https://hdl.handle.net/21.15107/rcub_vinar_13008

Orlovska I, Zmejkoski D, Khirunenko L, Kozyrovska N. A composite of bacterial cellulose and lignin for a healing of incurable wounds. in Integrative Biology & Medicine : Program and Abstracts. 2017;:53-53.
https://hdl.handle.net/21.15107/rcub_vinar_13008 .

Orlovska, Iryna, Zmejkoski, Danica, Khirunenko, L., Kozyrovska, N., "A composite of bacterial cellulose and lignin for a healing of incurable wounds" in Integrative Biology & Medicine : Program and Abstracts (2017):53-53,
https://hdl.handle.net/21.15107/rcub_vinar_13008 .