TY  - JOUR
AU  - Suljovrujić, Edin H.
AU  - Krstić, Maja
AU  - Rogić Miladinović, Zorana
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Stamboliev, Georgi
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11063
AB  - Hydrogels with oligo(ethylene glycol) (OEG), oligo(propylene glycol) (OPG), and for the first time with combined OEG/OPG pendant chains within the methacrylate (MA) network, were synthesized and the swelling behavior, thermal properties, microstructure, and genotoxicity were investigated. Prior to hydrogel fabrication, an optimized method was developed for oligo(propylene glycol) methacrylate (OPGMA), i.e., oligomer with an LCST below a temperature at which synthesis occurs, indicating that proper preparation and tuning of reaction conditions were required. PEG6MA and PPG5MA homopolymers, as well as P(EG6/PG5)MA copolymer hydrogel, were produced by gamma radiation-induced polymerization and crosslinking of OEG and OPG monomers from the monomer-solvent mixture by using different water/ethanol composition as a solvent and by exposing the reaction mixture to various radiation doses. The combination of OEG and OPG pendant chains within the same network was advantageous in that it allowed an easy tuning of the phase transition temperature. Thus, the volume phase transition (VPT) at temperatures above 70 °C observed in the case of PEG6MA, and in the case of PPG5MA at temperatures below 15 °C, could easily be tuned close to physiological temperatures for P(EG6/PG5)MA hydrogel. Finally, all obtained thermoresponsive hydrogels showed non-genotoxic and non-cytotoxic properties, which indicate promising potential for biomedical applications.
T2  - Reactive and Functional Polymers
T1  - Optimization of thermoresponsive hydrogels based on oligomers with lower critical solution temperature (LCST) far below/above physiological temperatures for biomedical applications
VL  - 189
SP  - 105612
DO  - 10.1016/j.reactfunctpolym.2023.105612
ER  - 

@article{
author = "Suljovrujić, Edin H. and Krstić, Maja and Rogić Miladinović, Zorana and Petrović, Sandra and Leskovac, Andreja and Stamboliev, Georgi",
year = "2023",
abstract = "Hydrogels with oligo(ethylene glycol) (OEG), oligo(propylene glycol) (OPG), and for the first time with combined OEG/OPG pendant chains within the methacrylate (MA) network, were synthesized and the swelling behavior, thermal properties, microstructure, and genotoxicity were investigated. Prior to hydrogel fabrication, an optimized method was developed for oligo(propylene glycol) methacrylate (OPGMA), i.e., oligomer with an LCST below a temperature at which synthesis occurs, indicating that proper preparation and tuning of reaction conditions were required. PEG6MA and PPG5MA homopolymers, as well as P(EG6/PG5)MA copolymer hydrogel, were produced by gamma radiation-induced polymerization and crosslinking of OEG and OPG monomers from the monomer-solvent mixture by using different water/ethanol composition as a solvent and by exposing the reaction mixture to various radiation doses. The combination of OEG and OPG pendant chains within the same network was advantageous in that it allowed an easy tuning of the phase transition temperature. Thus, the volume phase transition (VPT) at temperatures above 70 °C observed in the case of PEG6MA, and in the case of PPG5MA at temperatures below 15 °C, could easily be tuned close to physiological temperatures for P(EG6/PG5)MA hydrogel. Finally, all obtained thermoresponsive hydrogels showed non-genotoxic and non-cytotoxic properties, which indicate promising potential for biomedical applications.",
journal = "Reactive and Functional Polymers",
title = "Optimization of thermoresponsive hydrogels based on oligomers with lower critical solution temperature (LCST) far below/above physiological temperatures for biomedical applications",
volume = "189",
pages = "105612",
doi = "10.1016/j.reactfunctpolym.2023.105612"
}

Suljovrujić, E. H., Krstić, M., Rogić Miladinović, Z., Petrović, S., Leskovac, A.,& Stamboliev, G.. (2023). Optimization of thermoresponsive hydrogels based on oligomers with lower critical solution temperature (LCST) far below/above physiological temperatures for biomedical applications. in Reactive and Functional Polymers, 189, 105612.
https://doi.org/10.1016/j.reactfunctpolym.2023.105612

Suljovrujić EH, Krstić M, Rogić Miladinović Z, Petrović S, Leskovac A, Stamboliev G. Optimization of thermoresponsive hydrogels based on oligomers with lower critical solution temperature (LCST) far below/above physiological temperatures for biomedical applications. in Reactive and Functional Polymers. 2023;189:105612.
doi:10.1016/j.reactfunctpolym.2023.105612 .

Suljovrujić, Edin H., Krstić, Maja, Rogić Miladinović, Zorana, Petrović, Sandra, Leskovac, Andreja, Stamboliev, Georgi, "Optimization of thermoresponsive hydrogels based on oligomers with lower critical solution temperature (LCST) far below/above physiological temperatures for biomedical applications" in Reactive and Functional Polymers, 189 (2023):105612,
https://doi.org/10.1016/j.reactfunctpolym.2023.105612 . .

TY  - JOUR
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11388
AB  - While recognizing the gaps in pesticide regulations that impact consumer safety, public health concerns associated with pesticide contamination of foods are pointed out. The strategies and research directions proposed to prevent and/or reduce pesticide adverse effects on human health and the environment are discussed. Special attention is paid to organophosphate pesticides, as widely applied insecticides in agriculture, veterinary practices, and urban areas. Biotic and abiotic strategies for organophosphate pesticide degradation are discussed from a food safety perspective, indicating associated challenges and potential for further improvements. As food systems are endangered globally by unprecedented challenges, there is an urgent need to globally harmonize pesticide regulations and improve methodologies in the area of food safety to protect human health.
T2  - Foods
T1  - Pesticide Use and Degradation Strategies: Food Safety, Challenges and Perspectives
VL  - 12
IS  - 14
SP  - 2709
DO  - 10.3390/foods12142709
ER  - 

@article{
author = "Leskovac, Andreja and Petrović, Sandra",
year = "2023",
abstract = "While recognizing the gaps in pesticide regulations that impact consumer safety, public health concerns associated with pesticide contamination of foods are pointed out. The strategies and research directions proposed to prevent and/or reduce pesticide adverse effects on human health and the environment are discussed. Special attention is paid to organophosphate pesticides, as widely applied insecticides in agriculture, veterinary practices, and urban areas. Biotic and abiotic strategies for organophosphate pesticide degradation are discussed from a food safety perspective, indicating associated challenges and potential for further improvements. As food systems are endangered globally by unprecedented challenges, there is an urgent need to globally harmonize pesticide regulations and improve methodologies in the area of food safety to protect human health.",
journal = "Foods",
title = "Pesticide Use and Degradation Strategies: Food Safety, Challenges and Perspectives",
volume = "12",
number = "14",
pages = "2709",
doi = "10.3390/foods12142709"
}

Leskovac, A.,& Petrović, S.. (2023). Pesticide Use and Degradation Strategies: Food Safety, Challenges and Perspectives. in Foods, 12(14), 2709.
https://doi.org/10.3390/foods12142709

Leskovac A, Petrović S. Pesticide Use and Degradation Strategies: Food Safety, Challenges and Perspectives. in Foods. 2023;12(14):2709.
doi:10.3390/foods12142709 .

Leskovac, Andreja, Petrović, Sandra, "Pesticide Use and Degradation Strategies: Food Safety, Challenges and Perspectives" in Foods, 12, no. 14 (2023):2709,
https://doi.org/10.3390/foods12142709 . .

TY  - CHAP
AU  - Petrović, Sandra
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12050
AB  - Phospholipase D (PLD) enzyme is considered a critical regulator of numerous aspects of cell biology and signal transduction pathways. Deregulation of the expression and activity of PLDs has been linked to numerous diseases, including cancer. PLD isoforms and their hydrolysate phosphatidic acid are involved in multiple stages of tumorigenesis, angiogenesis, and metastasis. Numerous in vitro and in vivo studies performed thus far highlighted PLD inhibition as a promising therapeutic strategy against cancer. Considerable research efforts have been dedicated to structure-guided inhibitor design and development of specific potent and nontoxic PLD inhibitors, which may impede multiple aspects of cancer growth and invasion by acting either as a single agent or in combination with chemotherapy/radiotherapy. Based on the established knowledge of these subjects, the therapeutic potentials and limitations of PLD inhibitors application in targeted cancer therapy are discussed in this chapter.
PB  - Elsevier
T2  - Phospholipases in Physiology and Pathology
T1  - Phospholipase D inhibitors for targeted cancer therapy: Potentials and limitations
VL  - 5
SP  - 255
EP  - 265
DO  - 10.1016/B978-0-323-95699-4.00014-1
ER  - 

@inbook{
author = "Petrović, Sandra",
year = "2023",
abstract = "Phospholipase D (PLD) enzyme is considered a critical regulator of numerous aspects of cell biology and signal transduction pathways. Deregulation of the expression and activity of PLDs has been linked to numerous diseases, including cancer. PLD isoforms and their hydrolysate phosphatidic acid are involved in multiple stages of tumorigenesis, angiogenesis, and metastasis. Numerous in vitro and in vivo studies performed thus far highlighted PLD inhibition as a promising therapeutic strategy against cancer. Considerable research efforts have been dedicated to structure-guided inhibitor design and development of specific potent and nontoxic PLD inhibitors, which may impede multiple aspects of cancer growth and invasion by acting either as a single agent or in combination with chemotherapy/radiotherapy. Based on the established knowledge of these subjects, the therapeutic potentials and limitations of PLD inhibitors application in targeted cancer therapy are discussed in this chapter.",
publisher = "Elsevier",
journal = "Phospholipases in Physiology and Pathology",
booktitle = "Phospholipase D inhibitors for targeted cancer therapy: Potentials and limitations",
volume = "5",
pages = "255-265",
doi = "10.1016/B978-0-323-95699-4.00014-1"
}

Petrović, S.. (2023). Phospholipase D inhibitors for targeted cancer therapy: Potentials and limitations. in Phospholipases in Physiology and Pathology
Elsevier., 5, 255-265.
https://doi.org/10.1016/B978-0-323-95699-4.00014-1

Petrović S. Phospholipase D inhibitors for targeted cancer therapy: Potentials and limitations. in Phospholipases in Physiology and Pathology. 2023;5:255-265.
doi:10.1016/B978-0-323-95699-4.00014-1 .

Petrović, Sandra, "Phospholipase D inhibitors for targeted cancer therapy: Potentials and limitations" in Phospholipases in Physiology and Pathology, 5 (2023):255-265,
https://doi.org/10.1016/B978-0-323-95699-4.00014-1 . .

TY  - CHAP
AU  - Petrović, Sandra
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10458
AB  - Although an extensive legal framework regulates the development of organophosphate pesticides (OP), serious concerns have been raised considering human health risks resulting from consumption and acute or/and chronic exposure. Most pesticides and their residues are toxic to both the environment and humans, so international and national regulatory bodies have been established to control and enforce pesticide regulations and the maximum concentrations of residues in food and feed that are considered safe for humans. However, excessive pesticide application increases the risk of combined exposures to multiple residues that may lead to serious cumulative toxicity and adverse effects on human health. The health hazards can range from short-term to chronic conditions. For most of the OPs, toxicological data are collected from numerous studies covering a wide range of toxicological endpoints; however, the risk of health hazards due to pesticide exposure depends not only on how toxic the compounds are but also on the dose level at which harmful effects might be observed, and the frequency, timing and levels of exposure. This chapter presents the current state of knowledge and future perspectives regarding OPs use, regulations, routes of exposure, and the concomitant impacts on human health. © 2022 Nova Science Publishers, Inc.
T2  - Organophosphates: Detection, Exposure and Occurrence. Volume 1: Impact on Health and the Natural Environment
T1  - Organophosphate Pesticides and Human Health: Current Knowledge and Future Prospects
SP  - 1
EP  - 26
UR  - https://hdl.handle.net/21.15107/rcub_vinar_10458
ER  - 

@inbook{
author = "Petrović, Sandra",
year = "2022",
abstract = "Although an extensive legal framework regulates the development of organophosphate pesticides (OP), serious concerns have been raised considering human health risks resulting from consumption and acute or/and chronic exposure. Most pesticides and their residues are toxic to both the environment and humans, so international and national regulatory bodies have been established to control and enforce pesticide regulations and the maximum concentrations of residues in food and feed that are considered safe for humans. However, excessive pesticide application increases the risk of combined exposures to multiple residues that may lead to serious cumulative toxicity and adverse effects on human health. The health hazards can range from short-term to chronic conditions. For most of the OPs, toxicological data are collected from numerous studies covering a wide range of toxicological endpoints; however, the risk of health hazards due to pesticide exposure depends not only on how toxic the compounds are but also on the dose level at which harmful effects might be observed, and the frequency, timing and levels of exposure. This chapter presents the current state of knowledge and future perspectives regarding OPs use, regulations, routes of exposure, and the concomitant impacts on human health. © 2022 Nova Science Publishers, Inc.",
journal = "Organophosphates: Detection, Exposure and Occurrence. Volume 1: Impact on Health and the Natural Environment",
booktitle = "Organophosphate Pesticides and Human Health: Current Knowledge and Future Prospects",
pages = "1-26",
url = "https://hdl.handle.net/21.15107/rcub_vinar_10458"
}

Petrović, S.. (2022). Organophosphate Pesticides and Human Health: Current Knowledge and Future Prospects. in Organophosphates: Detection, Exposure and Occurrence. Volume 1: Impact on Health and the Natural Environment, 1-26.
https://hdl.handle.net/21.15107/rcub_vinar_10458

Petrović S. Organophosphate Pesticides and Human Health: Current Knowledge and Future Prospects. in Organophosphates: Detection, Exposure and Occurrence. Volume 1: Impact on Health and the Natural Environment. 2022;:1-26.
https://hdl.handle.net/21.15107/rcub_vinar_10458 .

Petrović, Sandra, "Organophosphate Pesticides and Human Health: Current Knowledge and Future Prospects" in Organophosphates: Detection, Exposure and Occurrence. Volume 1: Impact on Health and the Natural Environment (2022):1-26,
https://hdl.handle.net/21.15107/rcub_vinar_10458 .

TY  - CHAP
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10649
AB  - While recognizing the differences in the pathogenesis of different malignancies, the outstanding body of evidence has been accumulated in the past few decades, implicating the role of oxidative stress in the process of cancer initiation and progression. Cancer cells exhibit an altered energy metabolism and highly efficient redox systems that enable rapid cell proliferation and survival in various conditions. The adaptation/resistance of cancer cells to chronic oxidative stress through different mechanisms usually leads to drug resistance. The specific disruption of the redox capacity with either scavenging the excessive intracellular ROS or inducing ROS generation through exogenous oxidative insult represents a promising approach for cancer therapy. A considerable research effort has been dedicated to identifying the therapeutic agents with redox-modulation capacity that may impede cancer. Based on the established knowledge in these subjects, the natural and synthetic redox modulators currently used or under investigation for their potential application in the oxidative stress-targeted cancer therapy are reviewed.
T2  - Handbook of Oxidative Stress in Cancer: Mechanistic Aspects
T1  - Prospective Application of Natural and Synthetic Redox Modulators in Oxidative Stress-Targeted Cancer Therapy
SP  - 2101
EP  - 2121
DO  - 10.1007/978-981-15-9411-3_130
ER  - 

@inbook{
author = "Petrović, Sandra and Leskovac, Andreja",
year = "2022",
abstract = "While recognizing the differences in the pathogenesis of different malignancies, the outstanding body of evidence has been accumulated in the past few decades, implicating the role of oxidative stress in the process of cancer initiation and progression. Cancer cells exhibit an altered energy metabolism and highly efficient redox systems that enable rapid cell proliferation and survival in various conditions. The adaptation/resistance of cancer cells to chronic oxidative stress through different mechanisms usually leads to drug resistance. The specific disruption of the redox capacity with either scavenging the excessive intracellular ROS or inducing ROS generation through exogenous oxidative insult represents a promising approach for cancer therapy. A considerable research effort has been dedicated to identifying the therapeutic agents with redox-modulation capacity that may impede cancer. Based on the established knowledge in these subjects, the natural and synthetic redox modulators currently used or under investigation for their potential application in the oxidative stress-targeted cancer therapy are reviewed.",
journal = "Handbook of Oxidative Stress in Cancer: Mechanistic Aspects",
booktitle = "Prospective Application of Natural and Synthetic Redox Modulators in Oxidative Stress-Targeted Cancer Therapy",
pages = "2101-2121",
doi = "10.1007/978-981-15-9411-3_130"
}

Petrović, S.,& Leskovac, A.. (2022). Prospective Application of Natural and Synthetic Redox Modulators in Oxidative Stress-Targeted Cancer Therapy. in Handbook of Oxidative Stress in Cancer: Mechanistic Aspects, 2101-2121.
https://doi.org/10.1007/978-981-15-9411-3_130

Petrović S, Leskovac A. Prospective Application of Natural and Synthetic Redox Modulators in Oxidative Stress-Targeted Cancer Therapy. in Handbook of Oxidative Stress in Cancer: Mechanistic Aspects. 2022;:2101-2121.
doi:10.1007/978-981-15-9411-3_130 .

Petrović, Sandra, Leskovac, Andreja, "Prospective Application of Natural and Synthetic Redox Modulators in Oxidative Stress-Targeted Cancer Therapy" in Handbook of Oxidative Stress in Cancer: Mechanistic Aspects (2022):2101-2121,
https://doi.org/10.1007/978-981-15-9411-3_130 . .

TY  - CONF
AU  - Valenta Šobot, Ana
AU  - Filipović Tričković, Jelena
AU  - Drakulić, Dunja
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Momić, Tatjana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11582
AB  - Sweroside (Sw) is a secondary metabolite commonly found in plants belonging to the Gentianaceae family [1]. This iridoid compound is well-known for its anti-inflammatory [2], antidiabetic [3] and antitumor properties [4], which have been studied in pathological model systems. In transformed cell lines, Sw displays an antitumor effect by apoptosis activation [5]. Since healthy cells are also exposed to cancer therapy applied in vivo, our goal was to determine Sw’s cytotoxic concentration in primary human peripheral blood mononuclear cells (PBMCs) after 48 h of treatment with a range of concentration from 20 μM to 130 μM Sw in vitro, and to analyze whether the cytotoxic effect was due to activated apoptosis. According to the obtained results of the trypan blue dye exclusion test, 48 h of treatment with 50 μM Sw and higher concentrations led to a significant decrease in cell number. The DNA fragmentation assay indicated that following 50 μM Sw treatment, cells are dying in an apoptosis-like manner since the level of DNA fragments was 3.5 times higher than in the untreated control. The type of cell death was confirmed by immunoblot analysis of apoptosis- specific protein markers, which revealed the elevation of cleaved caspase-3 and PARP1 89 kDa fragments. Our findings showed that like in transformed cell lines, Sw in healthy cells can also activate apoptosis. A potential difference in sensitivity to Sw treatment between healthy and transformed cells could justify Sw treatment in anticancer therapy.
PB  - Belgrade : University of Belgrade, Faculty of Agriculture
C3  - 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts
T1  - Sweroside displays a cytotoxic effect by activating apoptosis in human peripheral blood mononuclear cells
SP  - 47
EP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11582
ER  - 

@conference{
author = "Valenta Šobot, Ana and Filipović Tričković, Jelena and Drakulić, Dunja and Leskovac, Andreja and Petrović, Sandra and Momić, Tatjana",
year = "2022",
abstract = "Sweroside (Sw) is a secondary metabolite commonly found in plants belonging to the Gentianaceae family [1]. This iridoid compound is well-known for its anti-inflammatory [2], antidiabetic [3] and antitumor properties [4], which have been studied in pathological model systems. In transformed cell lines, Sw displays an antitumor effect by apoptosis activation [5]. Since healthy cells are also exposed to cancer therapy applied in vivo, our goal was to determine Sw’s cytotoxic concentration in primary human peripheral blood mononuclear cells (PBMCs) after 48 h of treatment with a range of concentration from 20 μM to 130 μM Sw in vitro, and to analyze whether the cytotoxic effect was due to activated apoptosis. According to the obtained results of the trypan blue dye exclusion test, 48 h of treatment with 50 μM Sw and higher concentrations led to a significant decrease in cell number. The DNA fragmentation assay indicated that following 50 μM Sw treatment, cells are dying in an apoptosis-like manner since the level of DNA fragments was 3.5 times higher than in the untreated control. The type of cell death was confirmed by immunoblot analysis of apoptosis- specific protein markers, which revealed the elevation of cleaved caspase-3 and PARP1 89 kDa fragments. Our findings showed that like in transformed cell lines, Sw in healthy cells can also activate apoptosis. A potential difference in sensitivity to Sw treatment between healthy and transformed cells could justify Sw treatment in anticancer therapy.",
publisher = "Belgrade : University of Belgrade, Faculty of Agriculture",
journal = "1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts",
title = "Sweroside displays a cytotoxic effect by activating apoptosis in human peripheral blood mononuclear cells",
pages = "47-47",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11582"
}

Valenta Šobot, A., Filipović Tričković, J., Drakulić, D., Leskovac, A., Petrović, S.,& Momić, T.. (2022). Sweroside displays a cytotoxic effect by activating apoptosis in human peripheral blood mononuclear cells. in 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts
Belgrade : University of Belgrade, Faculty of Agriculture., 47-47.
https://hdl.handle.net/21.15107/rcub_vinar_11582

Valenta Šobot A, Filipović Tričković J, Drakulić D, Leskovac A, Petrović S, Momić T. Sweroside displays a cytotoxic effect by activating apoptosis in human peripheral blood mononuclear cells. in 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts. 2022;:47-47.
https://hdl.handle.net/21.15107/rcub_vinar_11582 .

Valenta Šobot, Ana, Filipović Tričković, Jelena, Drakulić, Dunja, Leskovac, Andreja, Petrović, Sandra, Momić, Tatjana, "Sweroside displays a cytotoxic effect by activating apoptosis in human peripheral blood mononuclear cells" in 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts (2022):47-47,
https://hdl.handle.net/21.15107/rcub_vinar_11582 .

TY  - CONF
AU  - Valenta Šobot, Ana
AU  - Filipović Tričković, Jelena
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Momić, Tatjana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11583
AB  - Quercetin (Q) is one of the most common and well researched antioxidant flavonoids, which usually occurs in plant-based foods, and medicinal plants. It was shown that quercetin exerts many beneficial effects on human health, including prevention of cancer and heart diseases. Quercetin was found to be toxic toward various types of cancer cell, still, at higher concentrations it was also shown toxic toward normal human cells [1,2]. One of the approaches to overcome this shortcoming offers nanotechnology which enables the novel perspective of phytochemical usage in contemporary medicine [3]. The strategy of binding quercetin to the gold nanoparticles during their synthesis was used, which resulted in quercetin capped gold nanoparticles (NPQ) [4]. Trypan blue exclusion test [5] was used to evaluate peripheral blood mononuclear cells (PBMC) viability after their exposure to either NPQ or free Q during 24, 48 and 72 h, at 37 °C, in the range of quercetin concentrations from 5 to 50 μg/mL. A significant reduction in the cell count was observed in PBMC cultures treated with 10, 20, and 50 μg/mL of free Q, for all exposure times. The treatments of increasing concentrations and exposure times lowered the cells viability, resulting in 63% of the viable cells, following 72 h of the treatment with 50 μg/mL of free Q. Although NPQ treatments affected the cells viability in a concentration- and time-dependent manner the treatment with 50 μg/mL of NPQ for 72 h, had a milder effect on PBMC cultures than free Q, resulting in 81% of the viable cells (Figure 1). According to the obtained results, NPQ were shown less toxic toward PBMC than free Q.
PB  - Belgrade : University of Belgrade, Faculty of Agriculture
C3  - 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts
T1  - Nanotechnology approach for diminishing quercetin toxicity toward peripheral blood mononuclear cells
SP  - 64
EP  - 64
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11583
ER  - 

@conference{
author = "Valenta Šobot, Ana and Filipović Tričković, Jelena and Leskovac, Andreja and Petrović, Sandra and Momić, Tatjana",
year = "2022",
abstract = "Quercetin (Q) is one of the most common and well researched antioxidant flavonoids, which usually occurs in plant-based foods, and medicinal plants. It was shown that quercetin exerts many beneficial effects on human health, including prevention of cancer and heart diseases. Quercetin was found to be toxic toward various types of cancer cell, still, at higher concentrations it was also shown toxic toward normal human cells [1,2]. One of the approaches to overcome this shortcoming offers nanotechnology which enables the novel perspective of phytochemical usage in contemporary medicine [3]. The strategy of binding quercetin to the gold nanoparticles during their synthesis was used, which resulted in quercetin capped gold nanoparticles (NPQ) [4]. Trypan blue exclusion test [5] was used to evaluate peripheral blood mononuclear cells (PBMC) viability after their exposure to either NPQ or free Q during 24, 48 and 72 h, at 37 °C, in the range of quercetin concentrations from 5 to 50 μg/mL. A significant reduction in the cell count was observed in PBMC cultures treated with 10, 20, and 50 μg/mL of free Q, for all exposure times. The treatments of increasing concentrations and exposure times lowered the cells viability, resulting in 63% of the viable cells, following 72 h of the treatment with 50 μg/mL of free Q. Although NPQ treatments affected the cells viability in a concentration- and time-dependent manner the treatment with 50 μg/mL of NPQ for 72 h, had a milder effect on PBMC cultures than free Q, resulting in 81% of the viable cells (Figure 1). According to the obtained results, NPQ were shown less toxic toward PBMC than free Q.",
publisher = "Belgrade : University of Belgrade, Faculty of Agriculture",
journal = "1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts",
title = "Nanotechnology approach for diminishing quercetin toxicity toward peripheral blood mononuclear cells",
pages = "64-64",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11583"
}

Valenta Šobot, A., Filipović Tričković, J., Leskovac, A., Petrović, S.,& Momić, T.. (2022). Nanotechnology approach for diminishing quercetin toxicity toward peripheral blood mononuclear cells. in 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts
Belgrade : University of Belgrade, Faculty of Agriculture., 64-64.
https://hdl.handle.net/21.15107/rcub_vinar_11583

Valenta Šobot A, Filipović Tričković J, Leskovac A, Petrović S, Momić T. Nanotechnology approach for diminishing quercetin toxicity toward peripheral blood mononuclear cells. in 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts. 2022;:64-64.
https://hdl.handle.net/21.15107/rcub_vinar_11583 .

Valenta Šobot, Ana, Filipović Tričković, Jelena, Leskovac, Andreja, Petrović, Sandra, Momić, Tatjana, "Nanotechnology approach for diminishing quercetin toxicity toward peripheral blood mononuclear cells" in 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts (2022):64-64,
https://hdl.handle.net/21.15107/rcub_vinar_11583 .

TY  - CONF
AU  - Petrović, Sandra
AU  - Vujačić Nikezić, Ana V.
AU  - Čolović, Mirjana
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11689
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry
T1  - Effect of Chlorpyrifos-oxon on Membrane Damage and Cell Viability
SP  - 139
EP  - 142
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11689
ER  - 

@conference{
author = "Petrović, Sandra and Vujačić Nikezić, Ana V. and Čolović, Mirjana",
year = "2021",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry",
title = "Effect of Chlorpyrifos-oxon on Membrane Damage and Cell Viability",
pages = "139-142",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11689"
}

Petrović, S., Vujačić Nikezić, A. V.,& Čolović, M.. (2021). Effect of Chlorpyrifos-oxon on Membrane Damage and Cell Viability. in 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry
Belgrade : Vinča Institute of Nuclear Sciences., 139-142.
https://hdl.handle.net/21.15107/rcub_vinar_11689

Petrović S, Vujačić Nikezić AV, Čolović M. Effect of Chlorpyrifos-oxon on Membrane Damage and Cell Viability. in 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry. 2021;:139-142.
https://hdl.handle.net/21.15107/rcub_vinar_11689 .

Petrović, Sandra, Vujačić Nikezić, Ana V., Čolović, Mirjana, "Effect of Chlorpyrifos-oxon on Membrane Damage and Cell Viability" in 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry (2021):139-142,
https://hdl.handle.net/21.15107/rcub_vinar_11689 .

TY  - CONF
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12943
PB  - Belgrade : Vinča Institute of Nuclear Sciences - National Institute of the Republic of Serbia
T1  - Proceedings - 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia [is an online satellite event of] 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021
SP  - 1
EP  - 195
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12943
ER  - 

@conference{
editor = "Čolović, Mirjana, Petrović, Sandra",
year = "2021",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences - National Institute of the Republic of Serbia",
title = "Proceedings - 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia [is an online satellite event of] 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021",
pages = "1-195",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12943"
}

Čolović, M.,& Petrović, S.. (2021). Proceedings - 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia [is an online satellite event of] 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021. 
Belgrade : Vinča Institute of Nuclear Sciences - National Institute of the Republic of Serbia., 1-195.
https://hdl.handle.net/21.15107/rcub_vinar_12943

Čolović M, Petrović S. Proceedings - 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia [is an online satellite event of] 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021. 2021;:1-195.
https://hdl.handle.net/21.15107/rcub_vinar_12943 .

Čolović, Mirjana, Petrović, Sandra, "Proceedings - 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia [is an online satellite event of] 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021" (2021):1-195,
https://hdl.handle.net/21.15107/rcub_vinar_12943 .

TY  - CONF
AU  - Leskovac, Andreja
AU  - Čolović, Mirjana
AU  - Bondžić, Aleksandra
AU  - Petrović, Sandra
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11686
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry
T1  - N-Acetylcysteine as Regulator of the Cellular Homeostasis
SP  - 192
EP  - 195
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11686
ER  - 

@conference{
author = "Leskovac, Andreja and Čolović, Mirjana and Bondžić, Aleksandra and Petrović, Sandra",
year = "2021",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry",
title = "N-Acetylcysteine as Regulator of the Cellular Homeostasis",
pages = "192-195",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11686"
}

Leskovac, A., Čolović, M., Bondžić, A.,& Petrović, S.. (2021). N-Acetylcysteine as Regulator of the Cellular Homeostasis. in 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry
Belgrade : Vinča Institute of Nuclear Sciences., 192-195.
https://hdl.handle.net/21.15107/rcub_vinar_11686

Leskovac A, Čolović M, Bondžić A, Petrović S. N-Acetylcysteine as Regulator of the Cellular Homeostasis. in 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry. 2021;:192-195.
https://hdl.handle.net/21.15107/rcub_vinar_11686 .

Leskovac, Andreja, Čolović, Mirjana, Bondžić, Aleksandra, Petrović, Sandra, "N-Acetylcysteine as Regulator of the Cellular Homeostasis" in 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry (2021):192-195,
https://hdl.handle.net/21.15107/rcub_vinar_11686 .

TY  - JOUR
AU  - Stamenović, Una
AU  - Davidović, Slađana
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Stoiljković, Milovan
AU  - Vodnik, Vesna
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9874
AB  - Two silver–polyaniline/polyvinylpyrrolidone (Ag–PANI/PVP) nanocomposites were prepared using in situ integration of silver nanoparticles (AgNPs) during oxidative aniline polymerization, accelerated by the presence of PVP, which as well minimized the risk of particle agglomeration and macroscopic precipitation. Both nanocomposites have similar silver content (∼44 wt% Ag) but different morphological features of AgNPs (spheres/triangles) and polymers (granular/wrinkling pattern). Several spectroscopic, macroscopic, and analytical techniques, microscopy, and surface analysis methods have been used to analyze their physical and chemical properties. Investigation of their antimicrobial potential and possible application as an effective weapon against indicator microbial strains, E. coli, S. aureus, and C. albicans, has shown inhibition of microbial growth by more than 90%, even at low composite concentrations (5 ppm) and short contact time (4 h). A biosafety assessment of Ag–PANI/PVP that comprised testing the genotoxicity and redox modulating activity was performed using human peripheral blood cells as a model system. The obtained results have shown that the investigated Ag–PANI/PVP exhibited significant prooxidant and cytostatic effects (p < 0.05) with no apparent potential to induce DNA damage. Although precautions should be taken to protect human health, the significant antimicrobial efficiency of Ag–PANI/PVP makes it suitable for further studies and applications in non-medical areas, such as wastewater treatment.
T2  - New Journal of Chemistry
T1  - Antimicrobial and biological effects of polyaniline/polyvinylpyrrolidone nanocomposites loaded with silver nanospheres/triangles
VL  - 45
IS  - 28
SP  - 12711
EP  - 12720
DO  - 10.1039/D1NJ02729H
ER  - 

@article{
author = "Stamenović, Una and Davidović, Slađana and Petrović, Sandra and Leskovac, Andreja and Stoiljković, Milovan and Vodnik, Vesna",
year = "2021",
abstract = "Two silver–polyaniline/polyvinylpyrrolidone (Ag–PANI/PVP) nanocomposites were prepared using in situ integration of silver nanoparticles (AgNPs) during oxidative aniline polymerization, accelerated by the presence of PVP, which as well minimized the risk of particle agglomeration and macroscopic precipitation. Both nanocomposites have similar silver content (∼44 wt% Ag) but different morphological features of AgNPs (spheres/triangles) and polymers (granular/wrinkling pattern). Several spectroscopic, macroscopic, and analytical techniques, microscopy, and surface analysis methods have been used to analyze their physical and chemical properties. Investigation of their antimicrobial potential and possible application as an effective weapon against indicator microbial strains, E. coli, S. aureus, and C. albicans, has shown inhibition of microbial growth by more than 90%, even at low composite concentrations (5 ppm) and short contact time (4 h). A biosafety assessment of Ag–PANI/PVP that comprised testing the genotoxicity and redox modulating activity was performed using human peripheral blood cells as a model system. The obtained results have shown that the investigated Ag–PANI/PVP exhibited significant prooxidant and cytostatic effects (p < 0.05) with no apparent potential to induce DNA damage. Although precautions should be taken to protect human health, the significant antimicrobial efficiency of Ag–PANI/PVP makes it suitable for further studies and applications in non-medical areas, such as wastewater treatment.",
journal = "New Journal of Chemistry",
title = "Antimicrobial and biological effects of polyaniline/polyvinylpyrrolidone nanocomposites loaded with silver nanospheres/triangles",
volume = "45",
number = "28",
pages = "12711-12720",
doi = "10.1039/D1NJ02729H"
}

Stamenović, U., Davidović, S., Petrović, S., Leskovac, A., Stoiljković, M.,& Vodnik, V.. (2021). Antimicrobial and biological effects of polyaniline/polyvinylpyrrolidone nanocomposites loaded with silver nanospheres/triangles. in New Journal of Chemistry, 45(28), 12711-12720.
https://doi.org/10.1039/D1NJ02729H

Stamenović U, Davidović S, Petrović S, Leskovac A, Stoiljković M, Vodnik V. Antimicrobial and biological effects of polyaniline/polyvinylpyrrolidone nanocomposites loaded with silver nanospheres/triangles. in New Journal of Chemistry. 2021;45(28):12711-12720.
doi:10.1039/D1NJ02729H .

Stamenović, Una, Davidović, Slađana, Petrović, Sandra, Leskovac, Andreja, Stoiljković, Milovan, Vodnik, Vesna, "Antimicrobial and biological effects of polyaniline/polyvinylpyrrolidone nanocomposites loaded with silver nanospheres/triangles" in New Journal of Chemistry, 45, no. 28 (2021):12711-12720,
https://doi.org/10.1039/D1NJ02729H . .

TY  - JOUR
AU  - Ivković, Ivana
AU  - Bukvički, Danka
AU  - Novaković, Miroslav M.
AU  - Majstorović, Ivana
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Veljić, Milan
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10051
AB  - Liverworts are characterized by a high content of bioactive compounds reported to show antimicrobial, anticancer, and antioxidant properties. In this study, the biological effects of the methanol extract of the liverwort Pellia endiviifolia and its constituents, bis-bibenzyls perrottetin E, 10′-hydroxyperrottetin E, and 10,10′-dihydroxyperrottetin E, were investigated using human peripheral blood cells as a model system. The assessment of the investigated compounds comprised testing their genotoxicity, apoptotic potential, and redox modulating activities. The genotoxicity testing indicated that medium (25 µM) and high concentrations (100 µM) of the investigated compounds displayed genotoxic and antiproliferative effects in human lymphocytes as revealed by significant, concentration-dependent enhancement of the micronuclei incidence and decrease in the cytokinesis-block proliferation index compared to the control (P <.001). Analysis of leukocyte apoptosis showed a substantial potential of all investigated compounds to induce apoptosis, which was not concentration-dependent. The P endiviifolia extract and perrottetin E demonstrated considerable pro-apoptotic potential, even at the lowest concentration (1 µM) applied. Evaluation of the redox modulating effects, which comprised measuring erythrocyte catalase activity and the lymphocyte malondialdehyde level, showed that the investigated compounds did not induce oxidative stress in human peripheral blood cells (P >.05). The observed genotoxic, antiproliferative, and proapoptotic effects of the investigated compounds make them suitable for further comprehensive studies related to their possible applications as anticancer agents.
T2  - Natural Product Communications
T1  - Assessment of the Biological Effects of Pellia endiviifolia and its Constituents in Vitro
VL  - 16
IS  - 11
SP  - 1
DO  - 10.1177/1934578X211056422
ER  - 

@article{
author = "Ivković, Ivana and Bukvički, Danka and Novaković, Miroslav M. and Majstorović, Ivana and Leskovac, Andreja and Petrović, Sandra and Veljić, Milan",
year = "2021",
abstract = "Liverworts are characterized by a high content of bioactive compounds reported to show antimicrobial, anticancer, and antioxidant properties. In this study, the biological effects of the methanol extract of the liverwort Pellia endiviifolia and its constituents, bis-bibenzyls perrottetin E, 10′-hydroxyperrottetin E, and 10,10′-dihydroxyperrottetin E, were investigated using human peripheral blood cells as a model system. The assessment of the investigated compounds comprised testing their genotoxicity, apoptotic potential, and redox modulating activities. The genotoxicity testing indicated that medium (25 µM) and high concentrations (100 µM) of the investigated compounds displayed genotoxic and antiproliferative effects in human lymphocytes as revealed by significant, concentration-dependent enhancement of the micronuclei incidence and decrease in the cytokinesis-block proliferation index compared to the control (P <.001). Analysis of leukocyte apoptosis showed a substantial potential of all investigated compounds to induce apoptosis, which was not concentration-dependent. The P endiviifolia extract and perrottetin E demonstrated considerable pro-apoptotic potential, even at the lowest concentration (1 µM) applied. Evaluation of the redox modulating effects, which comprised measuring erythrocyte catalase activity and the lymphocyte malondialdehyde level, showed that the investigated compounds did not induce oxidative stress in human peripheral blood cells (P >.05). The observed genotoxic, antiproliferative, and proapoptotic effects of the investigated compounds make them suitable for further comprehensive studies related to their possible applications as anticancer agents.",
journal = "Natural Product Communications",
title = "Assessment of the Biological Effects of Pellia endiviifolia and its Constituents in Vitro",
volume = "16",
number = "11",
pages = "1",
doi = "10.1177/1934578X211056422"
}

Ivković, I., Bukvički, D., Novaković, M. M., Majstorović, I., Leskovac, A., Petrović, S.,& Veljić, M.. (2021). Assessment of the Biological Effects of Pellia endiviifolia and its Constituents in Vitro. in Natural Product Communications, 16(11), 1.
https://doi.org/10.1177/1934578X211056422

Ivković I, Bukvički D, Novaković MM, Majstorović I, Leskovac A, Petrović S, Veljić M. Assessment of the Biological Effects of Pellia endiviifolia and its Constituents in Vitro. in Natural Product Communications. 2021;16(11):1.
doi:10.1177/1934578X211056422 .

Ivković, Ivana, Bukvički, Danka, Novaković, Miroslav M., Majstorović, Ivana, Leskovac, Andreja, Petrović, Sandra, Veljić, Milan, "Assessment of the Biological Effects of Pellia endiviifolia and its Constituents in Vitro" in Natural Product Communications, 16, no. 11 (2021):1,
https://doi.org/10.1177/1934578X211056422 . .

TY  - CONF
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11581
PB  - Belgrade : Serbian Association for Cancer Research (SDIR)
C3  - SDIR-5 : 5th Congress of the Serbian Association for Cancer Research with international participation "Translational Potential of Cancer Research in Serbia" : Abstract Book
T1  - Ruthenium (II) complexes as promising candidates for cancer therapy
SP  - 74
EP  - 74
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11581
ER  - 

@conference{
author = "Leskovac, Andreja and Petrović, Sandra",
year = "2021",
publisher = "Belgrade : Serbian Association for Cancer Research (SDIR)",
journal = "SDIR-5 : 5th Congress of the Serbian Association for Cancer Research with international participation "Translational Potential of Cancer Research in Serbia" : Abstract Book",
title = "Ruthenium (II) complexes as promising candidates for cancer therapy",
pages = "74-74",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11581"
}

Leskovac, A.,& Petrović, S.. (2021). Ruthenium (II) complexes as promising candidates for cancer therapy. in SDIR-5 : 5th Congress of the Serbian Association for Cancer Research with international participation "Translational Potential of Cancer Research in Serbia" : Abstract Book
Belgrade : Serbian Association for Cancer Research (SDIR)., 74-74.
https://hdl.handle.net/21.15107/rcub_vinar_11581

Leskovac A, Petrović S. Ruthenium (II) complexes as promising candidates for cancer therapy. in SDIR-5 : 5th Congress of the Serbian Association for Cancer Research with international participation "Translational Potential of Cancer Research in Serbia" : Abstract Book. 2021;:74-74.
https://hdl.handle.net/21.15107/rcub_vinar_11581 .

Leskovac, Andreja, Petrović, Sandra, "Ruthenium (II) complexes as promising candidates for cancer therapy" in SDIR-5 : 5th Congress of the Serbian Association for Cancer Research with international participation "Translational Potential of Cancer Research in Serbia" : Abstract Book (2021):74-74,
https://hdl.handle.net/21.15107/rcub_vinar_11581 .

TY  - CONF
AU  - Petrović, Sandra
AU  - Bondžić, Aleksandra
AU  - Nastasijević, Branislav
AU  - Leskovac, Andreja
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11687
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry
T1  - Genotoxicity Testing of Acacia Honeys of Different Geographical Origin
SP  - 127
EP  - 130
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11687
ER  - 

@conference{
author = "Petrović, Sandra and Bondžić, Aleksandra and Nastasijević, Branislav and Leskovac, Andreja",
year = "2021",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry",
title = "Genotoxicity Testing of Acacia Honeys of Different Geographical Origin",
pages = "127-130",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11687"
}

Petrović, S., Bondžić, A., Nastasijević, B.,& Leskovac, A.. (2021). Genotoxicity Testing of Acacia Honeys of Different Geographical Origin. in 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry
Belgrade : Vinča Institute of Nuclear Sciences., 127-130.
https://hdl.handle.net/21.15107/rcub_vinar_11687

Petrović S, Bondžić A, Nastasijević B, Leskovac A. Genotoxicity Testing of Acacia Honeys of Different Geographical Origin. in 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry. 2021;:127-130.
https://hdl.handle.net/21.15107/rcub_vinar_11687 .

Petrović, Sandra, Bondžić, Aleksandra, Nastasijević, Branislav, Leskovac, Andreja, "Genotoxicity Testing of Acacia Honeys of Different Geographical Origin" in 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia, 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry (2021):127-130,
https://hdl.handle.net/21.15107/rcub_vinar_11687 .

TY  - JOUR
AU  - Laban, Bojana B.
AU  - Ralević, Uroš
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Vasić Anićijević, Dragana D.
AU  - Marković, Mirjana
AU  - Vasić, Vesna M.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8673
AB  - The simple green method for synthesis of stable L-Methionine (L-Met) capped silver (Ag@LM NPs) and gold (Au@LM NPs) nanoparticles (NPs) without adding any additional reduction agent or stabilizer was developed. Colloidal dispersions were characterized by UV–Vis spectrophotometry. The size and spherical shape of NPs were evaluated by transmission electron microscopy. Their surface covering was confirmed by atomic force microscopy, Fourier transform infrared spectroscopy, dynamic light scattering, and zeta potential measurements. Density functional theory calculations pointed that the preferential adsorption mode of L-Met on both Ag and Au surfaces was a vertical binding geometry via –NH2 group, while horizontal binding mode via [sbnd]S[sbnd] and –NH2 groups is also possible. The genotoxicity (evaluated by the micronucleus assay) of NPs, as well as their effects on some oxidative stress parameters (catalase activity, malondialdehyde level), were assessed in vitro using human peripheral blood cells as a model system. The influence of NPs on the morphology of lymphocyte cells studied using atomic force microscopy revealed that the membrane of cells remained unaffected after the treatment with NPs. When considering the effects of NPs on catalase activity and malondialdehyde level, neither particle type promoted oxidative stress. However, the treatment of lymphocytes with Ag@LM NPs induced a concentration-dependent enhancement of the micronuclei incidence and suppression of the cell proliferation while Au@LM NPs promoted cell proliferation, with no significant effects on micronuclei formation. The Ag@LM NPs were more prone to induce DNA damage than Au@LM NPs, which makes the latter type more suitable for further studies in nano-medicine. © 2019
T2  - Journal of Inorganic Biochemistry
T1  - Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles
VL  - 204
SP  - 110958
DO  - 10.1016/j.jinorgbio.2019.110958
ER  - 

@article{
author = "Laban, Bojana B. and Ralević, Uroš and Petrović, Sandra and Leskovac, Andreja and Vasić Anićijević, Dragana D. and Marković, Mirjana and Vasić, Vesna M.",
year = "2020",
abstract = "The simple green method for synthesis of stable L-Methionine (L-Met) capped silver (Ag@LM NPs) and gold (Au@LM NPs) nanoparticles (NPs) without adding any additional reduction agent or stabilizer was developed. Colloidal dispersions were characterized by UV–Vis spectrophotometry. The size and spherical shape of NPs were evaluated by transmission electron microscopy. Their surface covering was confirmed by atomic force microscopy, Fourier transform infrared spectroscopy, dynamic light scattering, and zeta potential measurements. Density functional theory calculations pointed that the preferential adsorption mode of L-Met on both Ag and Au surfaces was a vertical binding geometry via –NH2 group, while horizontal binding mode via [sbnd]S[sbnd] and –NH2 groups is also possible. The genotoxicity (evaluated by the micronucleus assay) of NPs, as well as their effects on some oxidative stress parameters (catalase activity, malondialdehyde level), were assessed in vitro using human peripheral blood cells as a model system. The influence of NPs on the morphology of lymphocyte cells studied using atomic force microscopy revealed that the membrane of cells remained unaffected after the treatment with NPs. When considering the effects of NPs on catalase activity and malondialdehyde level, neither particle type promoted oxidative stress. However, the treatment of lymphocytes with Ag@LM NPs induced a concentration-dependent enhancement of the micronuclei incidence and suppression of the cell proliferation while Au@LM NPs promoted cell proliferation, with no significant effects on micronuclei formation. The Ag@LM NPs were more prone to induce DNA damage than Au@LM NPs, which makes the latter type more suitable for further studies in nano-medicine. © 2019",
journal = "Journal of Inorganic Biochemistry",
title = "Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles",
volume = "204",
pages = "110958",
doi = "10.1016/j.jinorgbio.2019.110958"
}

Laban, B. B., Ralević, U., Petrović, S., Leskovac, A., Vasić Anićijević, D. D., Marković, M.,& Vasić, V. M.. (2020). Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles. in Journal of Inorganic Biochemistry, 204, 110958.
https://doi.org/10.1016/j.jinorgbio.2019.110958

Laban BB, Ralević U, Petrović S, Leskovac A, Vasić Anićijević DD, Marković M, Vasić VM. Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles. in Journal of Inorganic Biochemistry. 2020;204:110958.
doi:10.1016/j.jinorgbio.2019.110958 .

Laban, Bojana B., Ralević, Uroš, Petrović, Sandra, Leskovac, Andreja, Vasić Anićijević, Dragana D., Marković, Mirjana, Vasić, Vesna M., "Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles" in Journal of Inorganic Biochemistry, 204 (2020):110958,
https://doi.org/10.1016/j.jinorgbio.2019.110958 . .

TY  - JOUR
AU  - Bondžić, Aleksandra M.
AU  - Lazarević-Pašti, Tamara
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Čolović, Mirjana B.
AU  - Parac-Vogt, Tatjana N.
AU  - Janjić, Goran V.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9028
AB  - Acetylcholinesterase (AChE) inhibitors are important in the treatment of neurodegenerative diseases. Two inhibitors,12-tungstosilicic acid (WSiA) and 12-tungstophosphoric acid (WPA), which have polyoxometalate(POM) type structure, have been shown to inhibit AChE activity in nM concentration. Circular dichroism andtryptophan fluorescence spectroscopy demonstrated that the AChE inhibition was not accompanied by significantchanges in the secondary structure of the enzyme. The molecular docking approach has revealed a newallosteric binding site, termed β-allosteric site (β-AS), which is considered responsible for the inhibition of AChEby POMs. To the best of our knowledge, this is the first study reporting a new allosteric site that is consideredresponsible for AChE inhibition by voluminous and negatively charged molecules such as POMs. The selectedPOMs were further subjected to genotoxicity testing using human peripheral blood cells as a model system. Itwas shown that WSiA and WPA induced a mild cytostatic but not genotoxic effects in human lymphocytes, whichindicates their potential to be used as medicinal drugs. The identification of non-toxic compounds capable ofbinding to an allosteric site that so far has not been considered responsible for enzyme inhibition could befundamental for the development of new drug design strategies and the discovery of more efficient AChEmodulators.
T2  - European Journal of Pharmaceutical Sciences
T1  - A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition
VL  - 151
SP  - 105376
DO  - 10.1016/j.ejps.2020.105376
ER  - 

@article{
author = "Bondžić, Aleksandra M. and Lazarević-Pašti, Tamara and Leskovac, Andreja and Petrović, Sandra and Čolović, Mirjana B. and Parac-Vogt, Tatjana N. and Janjić, Goran V.",
year = "2020",
abstract = "Acetylcholinesterase (AChE) inhibitors are important in the treatment of neurodegenerative diseases. Two inhibitors,12-tungstosilicic acid (WSiA) and 12-tungstophosphoric acid (WPA), which have polyoxometalate(POM) type structure, have been shown to inhibit AChE activity in nM concentration. Circular dichroism andtryptophan fluorescence spectroscopy demonstrated that the AChE inhibition was not accompanied by significantchanges in the secondary structure of the enzyme. The molecular docking approach has revealed a newallosteric binding site, termed β-allosteric site (β-AS), which is considered responsible for the inhibition of AChEby POMs. To the best of our knowledge, this is the first study reporting a new allosteric site that is consideredresponsible for AChE inhibition by voluminous and negatively charged molecules such as POMs. The selectedPOMs were further subjected to genotoxicity testing using human peripheral blood cells as a model system. Itwas shown that WSiA and WPA induced a mild cytostatic but not genotoxic effects in human lymphocytes, whichindicates their potential to be used as medicinal drugs. The identification of non-toxic compounds capable ofbinding to an allosteric site that so far has not been considered responsible for enzyme inhibition could befundamental for the development of new drug design strategies and the discovery of more efficient AChEmodulators.",
journal = "European Journal of Pharmaceutical Sciences",
title = "A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition",
volume = "151",
pages = "105376",
doi = "10.1016/j.ejps.2020.105376"
}

Bondžić, A. M., Lazarević-Pašti, T., Leskovac, A., Petrović, S., Čolović, M. B., Parac-Vogt, T. N.,& Janjić, G. V.. (2020). A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition. in European Journal of Pharmaceutical Sciences, 151, 105376.
https://doi.org/10.1016/j.ejps.2020.105376

Bondžić AM, Lazarević-Pašti T, Leskovac A, Petrović S, Čolović MB, Parac-Vogt TN, Janjić GV. A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition. in European Journal of Pharmaceutical Sciences. 2020;151:105376.
doi:10.1016/j.ejps.2020.105376 .

Bondžić, Aleksandra M., Lazarević-Pašti, Tamara, Leskovac, Andreja, Petrović, Sandra, Čolović, Mirjana B., Parac-Vogt, Tatjana N., Janjić, Goran V., "A new acetylcholinesterase allosteric site responsible for binding voluminous negatively charged molecules – the role in the mechanism of AChE inhibition" in European Journal of Pharmaceutical Sciences, 151 (2020):105376,
https://doi.org/10.1016/j.ejps.2020.105376 . .

TY  - JOUR
AU  - Savić, Jasmina
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Lazarević-Pašti, Tamara
AU  - Nastasijević, Branislav J.
AU  - Tanović, Brankica B.
AU  - Gašić, Slavica M.
AU  - Vasić, Vesna M.
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0308814618313670
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7821
AB  - UV-C irradiation is widely used in the food industry. However, the health effects from dietary exposure to the irradiated pesticide residues retained in foodstuffs are underestimated. In this study, technical chlorpyrifos (TCPF) and its oil in water (EW) and emulsifiable concentrate (EC) formulations were irradiated by UV-C, and their photodegradation products were subjected to toxicity assessment, including determination of acetylcholinesterase (AChE) activity, genotoxicity and oxidative stress using human blood cells as a model system. Toxicity studies were performed using the chlorpyrifos concentrations in the range of those proposed as the maximum residue levels in plant commodities. TCPF, EW and EC photodegradation products induced DNA damage and oxidative stress, and their genotoxicity did not decrease as a function of irradiation time. Irradiated TCPF and EC are more potent AChE inhibitors than irradiated EW. Accordingly, the application of UV-C irradiation must be considered when processing the plants previously treated with chlorpyrifos formulations. © 2018 Elsevier Ltd
T2  - Food Chemistry
T1  - UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations
VL  - 271
SP  - 469
EP  - 478
DO  - 10.1016/j.foodchem.2018.07.207
ER  - 

@article{
author = "Savić, Jasmina and Petrović, Sandra and Leskovac, Andreja and Lazarević-Pašti, Tamara and Nastasijević, Branislav J. and Tanović, Brankica B. and Gašić, Slavica M. and Vasić, Vesna M.",
year = "2019",
abstract = "UV-C irradiation is widely used in the food industry. However, the health effects from dietary exposure to the irradiated pesticide residues retained in foodstuffs are underestimated. In this study, technical chlorpyrifos (TCPF) and its oil in water (EW) and emulsifiable concentrate (EC) formulations were irradiated by UV-C, and their photodegradation products were subjected to toxicity assessment, including determination of acetylcholinesterase (AChE) activity, genotoxicity and oxidative stress using human blood cells as a model system. Toxicity studies were performed using the chlorpyrifos concentrations in the range of those proposed as the maximum residue levels in plant commodities. TCPF, EW and EC photodegradation products induced DNA damage and oxidative stress, and their genotoxicity did not decrease as a function of irradiation time. Irradiated TCPF and EC are more potent AChE inhibitors than irradiated EW. Accordingly, the application of UV-C irradiation must be considered when processing the plants previously treated with chlorpyrifos formulations. © 2018 Elsevier Ltd",
journal = "Food Chemistry",
title = "UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations",
volume = "271",
pages = "469-478",
doi = "10.1016/j.foodchem.2018.07.207"
}

Savić, J., Petrović, S., Leskovac, A., Lazarević-Pašti, T., Nastasijević, B. J., Tanović, B. B., Gašić, S. M.,& Vasić, V. M.. (2019). UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations. in Food Chemistry, 271, 469-478.
https://doi.org/10.1016/j.foodchem.2018.07.207

Savić J, Petrović S, Leskovac A, Lazarević-Pašti T, Nastasijević BJ, Tanović BB, Gašić SM, Vasić VM. UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations. in Food Chemistry. 2019;271:469-478.
doi:10.1016/j.foodchem.2018.07.207 .

Savić, Jasmina, Petrović, Sandra, Leskovac, Andreja, Lazarević-Pašti, Tamara, Nastasijević, Branislav J., Tanović, Brankica B., Gašić, Slavica M., Vasić, Vesna M., "UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations" in Food Chemistry, 271 (2019):469-478,
https://doi.org/10.1016/j.foodchem.2018.07.207 . .

TY  - DATA
AU  - Savić, Jasmina
AU  - Petrović, Sandra
AU  - Leskovac, Andreja
AU  - Lazarević-Pašti, Tamara
AU  - Nastasijević, Branislav J.
AU  - Tanović, Brankica B.
AU  - Gašić, Slavica M.
AU  - Vasić, Vesna M.
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0308814618313670
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7831
AB  - Supplementary data 1: Table 1S. The chromatographic gradient profile; Table 2S. CPF concentration decrease (corresponding initial CPF concentration decrease was set as 0%) for all three forms of CPF depending on irradiation time; Table 3S. CPF and CPO concentrations determined chromatographically for TCPF, EW and EC formulations, as the function of irradiation time; % of CPO comparing to initial CPF concentration in all three forms of CPF; 
Supplementary data 2: Material safety data sheet according to 1907/2006/EC, Article 31/version 1; 
Supplementary data 3: Material safety data sheet according to 1907/2006/EC, Article 31/version 4
T2  - Food Chemistry
T1  - Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations
VL  - 271
SP  - 469
EP  - 478
DO  - 10.1016/j.foodchem.2018.07.207
ER  - 

@misc{
author = "Savić, Jasmina and Petrović, Sandra and Leskovac, Andreja and Lazarević-Pašti, Tamara and Nastasijević, Branislav J. and Tanović, Brankica B. and Gašić, Slavica M. and Vasić, Vesna M.",
year = "2019",
abstract = "Supplementary data 1: Table 1S. The chromatographic gradient profile; Table 2S. CPF concentration decrease (corresponding initial CPF concentration decrease was set as 0%) for all three forms of CPF depending on irradiation time; Table 3S. CPF and CPO concentrations determined chromatographically for TCPF, EW and EC formulations, as the function of irradiation time; % of CPO comparing to initial CPF concentration in all three forms of CPF; 
Supplementary data 2: Material safety data sheet according to 1907/2006/EC, Article 31/version 1; 
Supplementary data 3: Material safety data sheet according to 1907/2006/EC, Article 31/version 4",
journal = "Food Chemistry",
title = "Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations",
volume = "271",
pages = "469-478",
doi = "10.1016/j.foodchem.2018.07.207"
}

Savić, J., Petrović, S., Leskovac, A., Lazarević-Pašti, T., Nastasijević, B. J., Tanović, B. B., Gašić, S. M.,& Vasić, V. M.. (2019). Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations. in Food Chemistry, 271, 469-478.
https://doi.org/10.1016/j.foodchem.2018.07.207

Savić J, Petrović S, Leskovac A, Lazarević-Pašti T, Nastasijević BJ, Tanović BB, Gašić SM, Vasić VM. Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations. in Food Chemistry. 2019;271:469-478.
doi:10.1016/j.foodchem.2018.07.207 .

Savić, Jasmina, Petrović, Sandra, Leskovac, Andreja, Lazarević-Pašti, Tamara, Nastasijević, Branislav J., Tanović, Brankica B., Gašić, Slavica M., Vasić, Vesna M., "Supplementary data: UV-C light irradiation enhances toxic effects of chlorpyrifos and its formulations" in Food Chemistry, 271 (2019):469-478,
https://doi.org/10.1016/j.foodchem.2018.07.207 . .

TY  - JOUR
AU  - Bondžić, Aleksandra M.
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Vasić Anićijević, Dragana D.
AU  - Luce, Marco
AU  - Massai, Lara
AU  - Generosi, Amanda
AU  - Paci, Barbara
AU  - Cricenti, Antonio
AU  - Messori, Luigi
AU  - Vasić, Vesna M.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8747
AB  - Citrate-capped gold nanoparticles (AuNPs) were functionalized with three distinct antitumor gold(III) complexes, e.g., [Au(N,N)(OH)2][PF6], where (N,N)=2,2′-bipyridine; [Au(C,N)(AcO)2], where (C,N)=deprotonated 6-(1,1-dimethylbenzyl)-pyridine; [Au(C,N,N)(OH)][PF6], where (C,N,N)=deprotonated 6-(1,1-dimethylbenzyl)-2,2′-bipyridine, to assess the chance of tracking their subcellular distribution by atomic force microscopy (AFM), and surface enhanced Raman spectroscopy (SERS) techniques. An extensive physicochemical characterization of the formed conjugates was, thus, carried out by applying a variety of methods (density functional theory—DFT, UV/Vis spectrophotometry, AFM, Raman spectroscopy, and SERS). The resulting gold(III) complexes/AuNPs conjugates turned out to be pretty stable. Interestingly, they exhibited a dramatically increased resonance intensity in the Raman spectra induced by AuNPs. For testing the use of the functionalized AuNPs for biosensing, their distribution in the nuclear, cytosolic, and membrane cell fractions obtained from human lymphocytes was investigated by AFM and SERS. The conjugates were detected in the membrane and nuclear cell fractions but not in the cytosol. The AFM method confirmed that conjugates induced changes in the morphology and nanostructure of the membrane and nuclear fractions. The obtained results point out that the conjugates formed between AuNPs and gold(III) complexes may be used as a tool for tracking metallodrug distribution in the different cell fractions.
T2  - International Journal of Molecular Sciences
T1  - Conjugates of Gold Nanoparticles and Antitumor Gold(III) Complexes as a Tool for Their AFM and SERS Detection in Biological Tissue
VL  - 20
IS  - 24
SP  - 6306
DO  - 10.3390/ijms20246306
ER  - 

@article{
author = "Bondžić, Aleksandra M. and Leskovac, Andreja and Petrović, Sandra and Vasić Anićijević, Dragana D. and Luce, Marco and Massai, Lara and Generosi, Amanda and Paci, Barbara and Cricenti, Antonio and Messori, Luigi and Vasić, Vesna M.",
year = "2019",
abstract = "Citrate-capped gold nanoparticles (AuNPs) were functionalized with three distinct antitumor gold(III) complexes, e.g., [Au(N,N)(OH)2][PF6], where (N,N)=2,2′-bipyridine; [Au(C,N)(AcO)2], where (C,N)=deprotonated 6-(1,1-dimethylbenzyl)-pyridine; [Au(C,N,N)(OH)][PF6], where (C,N,N)=deprotonated 6-(1,1-dimethylbenzyl)-2,2′-bipyridine, to assess the chance of tracking their subcellular distribution by atomic force microscopy (AFM), and surface enhanced Raman spectroscopy (SERS) techniques. An extensive physicochemical characterization of the formed conjugates was, thus, carried out by applying a variety of methods (density functional theory—DFT, UV/Vis spectrophotometry, AFM, Raman spectroscopy, and SERS). The resulting gold(III) complexes/AuNPs conjugates turned out to be pretty stable. Interestingly, they exhibited a dramatically increased resonance intensity in the Raman spectra induced by AuNPs. For testing the use of the functionalized AuNPs for biosensing, their distribution in the nuclear, cytosolic, and membrane cell fractions obtained from human lymphocytes was investigated by AFM and SERS. The conjugates were detected in the membrane and nuclear cell fractions but not in the cytosol. The AFM method confirmed that conjugates induced changes in the morphology and nanostructure of the membrane and nuclear fractions. The obtained results point out that the conjugates formed between AuNPs and gold(III) complexes may be used as a tool for tracking metallodrug distribution in the different cell fractions.",
journal = "International Journal of Molecular Sciences",
title = "Conjugates of Gold Nanoparticles and Antitumor Gold(III) Complexes as a Tool for Their AFM and SERS Detection in Biological Tissue",
volume = "20",
number = "24",
pages = "6306",
doi = "10.3390/ijms20246306"
}

Bondžić, A. M., Leskovac, A., Petrović, S., Vasić Anićijević, D. D., Luce, M., Massai, L., Generosi, A., Paci, B., Cricenti, A., Messori, L.,& Vasić, V. M.. (2019). Conjugates of Gold Nanoparticles and Antitumor Gold(III) Complexes as a Tool for Their AFM and SERS Detection in Biological Tissue. in International Journal of Molecular Sciences, 20(24), 6306.
https://doi.org/10.3390/ijms20246306

Bondžić AM, Leskovac A, Petrović S, Vasić Anićijević DD, Luce M, Massai L, Generosi A, Paci B, Cricenti A, Messori L, Vasić VM. Conjugates of Gold Nanoparticles and Antitumor Gold(III) Complexes as a Tool for Their AFM and SERS Detection in Biological Tissue. in International Journal of Molecular Sciences. 2019;20(24):6306.
doi:10.3390/ijms20246306 .

Bondžić, Aleksandra M., Leskovac, Andreja, Petrović, Sandra, Vasić Anićijević, Dragana D., Luce, Marco, Massai, Lara, Generosi, Amanda, Paci, Barbara, Cricenti, Antonio, Messori, Luigi, Vasić, Vesna M., "Conjugates of Gold Nanoparticles and Antitumor Gold(III) Complexes as a Tool for Their AFM and SERS Detection in Biological Tissue" in International Journal of Molecular Sciences, 20, no. 24 (2019):6306,
https://doi.org/10.3390/ijms20246306 . .

TY  - JOUR
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Lazarević-Pašti, Tamara
AU  - Krstić, Milena P.
AU  - Vasić, Vesna M.
PY  - 2018
UR  - http://link.springer.com/10.1007/s00775-018-1560-x
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7765
AB  - In recent years, the search for effective anticancer compounds based on transition metal complexes has been the focus of medical investigations. The synergy between the ruthenium(II) and N-alkylphenothiazine counter-ions (chlorpromazine hydrochloride, thioridazine hydrochloride and trifluoperazine dihydrochloride, respectively) through the formation of three different complexes (1–3) was investigated. We explored whether the selected counter-ions and complexes might affect redox homeostasis and genome integrity of normal human blood cells, and induce an inhibition of Na+/K+-ATPase and AChE at pharmacologically relevant doses. Our results have shown that counter-ions and complexes did not affect the activity of Na+/K+-ATPase, while AChE activity was inhibited in a dose-dependent manner. All investigated compounds disturbed the viability and redox homeostasis of lymphocytes. Complexes 1 and 2 displayed potent cytotoxic and prooxidant action while complex 3 behaved as a weaker genotoxic inducer. Still, the tested complexes appeared to be less genotoxic and more cytostatic than the corresponding counter-ions. The effects of selected complexes were also tested in PC12 and U2OS cancer cells with special attention being given to the ability of phenothiazines to affect dopamine D2 receptors. Using the confocal laser scanning microscopy, we observed that all the complexes reduced cell viability. Although all investigated complexes have been bound to the dopamine receptor D2-eGFP, only complex 3 reduced its surface density and increased its lateral mobility in investigated cell lines. Albeit the role of alternative targets for complex 3 cannot be ruled out, its effects should be further examined as potential treatment strategy against cancer cells that overexpress D2.
T2  - JBIC Journal of Biological Inorganic Chemistry
T1  - Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents
VL  - 23
IS  - 5
SP  - 689
EP  - 704
DO  - 10.1007/s00775-018-1560-x
ER  - 

@article{
author = "Leskovac, Andreja and Petrović, Sandra and Lazarević-Pašti, Tamara and Krstić, Milena P. and Vasić, Vesna M.",
year = "2018",
abstract = "In recent years, the search for effective anticancer compounds based on transition metal complexes has been the focus of medical investigations. The synergy between the ruthenium(II) and N-alkylphenothiazine counter-ions (chlorpromazine hydrochloride, thioridazine hydrochloride and trifluoperazine dihydrochloride, respectively) through the formation of three different complexes (1–3) was investigated. We explored whether the selected counter-ions and complexes might affect redox homeostasis and genome integrity of normal human blood cells, and induce an inhibition of Na+/K+-ATPase and AChE at pharmacologically relevant doses. Our results have shown that counter-ions and complexes did not affect the activity of Na+/K+-ATPase, while AChE activity was inhibited in a dose-dependent manner. All investigated compounds disturbed the viability and redox homeostasis of lymphocytes. Complexes 1 and 2 displayed potent cytotoxic and prooxidant action while complex 3 behaved as a weaker genotoxic inducer. Still, the tested complexes appeared to be less genotoxic and more cytostatic than the corresponding counter-ions. The effects of selected complexes were also tested in PC12 and U2OS cancer cells with special attention being given to the ability of phenothiazines to affect dopamine D2 receptors. Using the confocal laser scanning microscopy, we observed that all the complexes reduced cell viability. Although all investigated complexes have been bound to the dopamine receptor D2-eGFP, only complex 3 reduced its surface density and increased its lateral mobility in investigated cell lines. Albeit the role of alternative targets for complex 3 cannot be ruled out, its effects should be further examined as potential treatment strategy against cancer cells that overexpress D2.",
journal = "JBIC Journal of Biological Inorganic Chemistry",
title = "Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents",
volume = "23",
number = "5",
pages = "689-704",
doi = "10.1007/s00775-018-1560-x"
}

Leskovac, A., Petrović, S., Lazarević-Pašti, T., Krstić, M. P.,& Vasić, V. M.. (2018). Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents. in JBIC Journal of Biological Inorganic Chemistry, 23(5), 689-704.
https://doi.org/10.1007/s00775-018-1560-x

Leskovac A, Petrović S, Lazarević-Pašti T, Krstić MP, Vasić VM. Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents. in JBIC Journal of Biological Inorganic Chemistry. 2018;23(5):689-704.
doi:10.1007/s00775-018-1560-x .

Leskovac, Andreja, Petrović, Sandra, Lazarević-Pašti, Tamara, Krstić, Milena P., Vasić, Vesna M., "Ruthenium(II)-N-alkyl phenothiazine complexes as potential anticancer agents" in JBIC Journal of Biological Inorganic Chemistry, 23, no. 5 (2018):689-704,
https://doi.org/10.1007/s00775-018-1560-x . .

TY  - JOUR
AU  - Bogdanović, Una
AU  - Dimitrijević, Suzana I.
AU  - Škapin, Srečo Davor
AU  - Popović, Maja
AU  - Rakočević, Zlatko Lj.
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Stoiljković, Milovan
AU  - Vodnik, Vesna
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0928493117326449
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7799
AB  - Copper nanoparticles (Cu NPs) have proven to own excellent antimicrobial efficacy, but the problems of easy oxidation and aggregation limit their practical application. Here, nanocomposite based on polyaniline (PANI) and Cu NPs solved this problem and brought additional physicochemical properties that are markedly advantageous for antimicrobial applications. Current work exploits this potential, to examine its time- and concentration-dependent antimicrobial activity, employing E. coli, S. aureus, and C. albicans as a model microbial species. Regarding the presence of polaronic charge carriers in the fibrous polyaniline network, effects of Cu NPs’ size and their partially oxidized surfaces (the data were confirmed by HRTEM, FESEM, XRD, Raman and XPS analysis), as well as rapid copper ions release, Cu-PANI nanocomposite showed efficient bactericidal and fungicidal activities at the concentrations ≤1 ppm, within the incubation time of 2 h. Beside the quantitative analysis, the high levels of cellular disruption for all tested microbes were evidenced by atomic force microscopy. Moreover, the minimum inhibitory and bactericidal concentrations of the Cu-PANI nanocomposite were lower than those reported for other nanocomposites. Using such low concentrations is recognized as a good way to avoid its toxicity toward the environment. For this purpose, Cu-PANI nanocomposite is tested for its genotoxicity and influence on the oxidative status of the human cells in vitro.
T2  - Materials Science and Engineering: C
T1  - Copper-polyaniline nanocomposite: Role of physicochemical properties on the antimicrobial activity and genotoxicity evaluation
VL  - 93
SP  - 49
EP  - 60
DO  - 10.1016/j.msec.2018.07.067
ER  - 

@article{
author = "Bogdanović, Una and Dimitrijević, Suzana I. and Škapin, Srečo Davor and Popović, Maja and Rakočević, Zlatko Lj. and Leskovac, Andreja and Petrović, Sandra and Stoiljković, Milovan and Vodnik, Vesna",
year = "2018",
abstract = "Copper nanoparticles (Cu NPs) have proven to own excellent antimicrobial efficacy, but the problems of easy oxidation and aggregation limit their practical application. Here, nanocomposite based on polyaniline (PANI) and Cu NPs solved this problem and brought additional physicochemical properties that are markedly advantageous for antimicrobial applications. Current work exploits this potential, to examine its time- and concentration-dependent antimicrobial activity, employing E. coli, S. aureus, and C. albicans as a model microbial species. Regarding the presence of polaronic charge carriers in the fibrous polyaniline network, effects of Cu NPs’ size and their partially oxidized surfaces (the data were confirmed by HRTEM, FESEM, XRD, Raman and XPS analysis), as well as rapid copper ions release, Cu-PANI nanocomposite showed efficient bactericidal and fungicidal activities at the concentrations ≤1 ppm, within the incubation time of 2 h. Beside the quantitative analysis, the high levels of cellular disruption for all tested microbes were evidenced by atomic force microscopy. Moreover, the minimum inhibitory and bactericidal concentrations of the Cu-PANI nanocomposite were lower than those reported for other nanocomposites. Using such low concentrations is recognized as a good way to avoid its toxicity toward the environment. For this purpose, Cu-PANI nanocomposite is tested for its genotoxicity and influence on the oxidative status of the human cells in vitro.",
journal = "Materials Science and Engineering: C",
title = "Copper-polyaniline nanocomposite: Role of physicochemical properties on the antimicrobial activity and genotoxicity evaluation",
volume = "93",
pages = "49-60",
doi = "10.1016/j.msec.2018.07.067"
}

Bogdanović, U., Dimitrijević, S. I., Škapin, S. D., Popović, M., Rakočević, Z. Lj., Leskovac, A., Petrović, S., Stoiljković, M.,& Vodnik, V.. (2018). Copper-polyaniline nanocomposite: Role of physicochemical properties on the antimicrobial activity and genotoxicity evaluation. in Materials Science and Engineering: C, 93, 49-60.
https://doi.org/10.1016/j.msec.2018.07.067

Bogdanović U, Dimitrijević SI, Škapin SD, Popović M, Rakočević ZL, Leskovac A, Petrović S, Stoiljković M, Vodnik V. Copper-polyaniline nanocomposite: Role of physicochemical properties on the antimicrobial activity and genotoxicity evaluation. in Materials Science and Engineering: C. 2018;93:49-60.
doi:10.1016/j.msec.2018.07.067 .

Bogdanović, Una, Dimitrijević, Suzana I., Škapin, Srečo Davor, Popović, Maja, Rakočević, Zlatko Lj., Leskovac, Andreja, Petrović, Sandra, Stoiljković, Milovan, Vodnik, Vesna, "Copper-polyaniline nanocomposite: Role of physicochemical properties on the antimicrobial activity and genotoxicity evaluation" in Materials Science and Engineering: C, 93 (2018):49-60,
https://doi.org/10.1016/j.msec.2018.07.067 . .

TY  - JOUR
AU  - Bondžić, Aleksandra M.
AU  - Čolović, Mirjana B.
AU  - Janjić, Goran V.
AU  - Zarić, Božidarka
AU  - Petrović, Sandra
AU  - Krstić, Danijela Z.
AU  - Marzo, Tiziano
AU  - Messori, Luigi
AU  - Vasić, Vesna M.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1648
AB  - The in vitro effects of oxo-bridged binuclear gold(III) complexes, i.e., [(bipy2Me)(2)Au-2(mu-O)(2)][PF6](2) (Auoxo6), Au-2[(bipydmb-H)(2)(mu-O)][PF6] (Au(2)bipyC) and [Au-2(phen(2Me))(2)(mu-O)(2)](PF6)(2) (Au(2)phen) on Na/K-ATPase, purified from the porcine cerebral cortex, were investigated. All three studied gold complexes inhibited the enzyme activity in a concentration-dependent manner achieving IC50 values in the low micromolar range. Kinetic analysis suggested an uncompetitive mode of inhibition for Auoxo6 and Au(2)bipyC, and a mixed type one for Au(2)phen. Docking studies indicated that the inhibitory actions of all tested complexes are related to E2-P enzyme conformation binding to ion channel and intracellular part between N and P sub-domain. In addition, Au(2)phen was able to inhibit the enzyme by interacting with its extracellular part as well. Toxic effects of the gold(III) complexes were evaluated in vitro by following lactate dehydrogenase activity in rat brain synaptosomes and incidence of micronuclei and cytokinesis-block proliferation index in cultivated human lymphocytes. All investigated complexes turned out to induce cytogenetic damage consisting of a significant decrease in cell proliferation and an increase in micronuclei in a dose-dependent manner. On the other hand, lactate dehydrogenase activity, an indicator of membrane integrity/viability, was not affected by Auoxo6 and Au(2)bipyC, while Au(2)phen slightly modified its activity.
T2  - Journal of Biological Inorganic Chemistry
T1  - The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach
VL  - 22
IS  - 6
SP  - 819
EP  - 832
DO  - 10.1007/s00775-017-1460-5
ER  - 

@article{
author = "Bondžić, Aleksandra M. and Čolović, Mirjana B. and Janjić, Goran V. and Zarić, Božidarka and Petrović, Sandra and Krstić, Danijela Z. and Marzo, Tiziano and Messori, Luigi and Vasić, Vesna M.",
year = "2017",
abstract = "The in vitro effects of oxo-bridged binuclear gold(III) complexes, i.e., [(bipy2Me)(2)Au-2(mu-O)(2)][PF6](2) (Auoxo6), Au-2[(bipydmb-H)(2)(mu-O)][PF6] (Au(2)bipyC) and [Au-2(phen(2Me))(2)(mu-O)(2)](PF6)(2) (Au(2)phen) on Na/K-ATPase, purified from the porcine cerebral cortex, were investigated. All three studied gold complexes inhibited the enzyme activity in a concentration-dependent manner achieving IC50 values in the low micromolar range. Kinetic analysis suggested an uncompetitive mode of inhibition for Auoxo6 and Au(2)bipyC, and a mixed type one for Au(2)phen. Docking studies indicated that the inhibitory actions of all tested complexes are related to E2-P enzyme conformation binding to ion channel and intracellular part between N and P sub-domain. In addition, Au(2)phen was able to inhibit the enzyme by interacting with its extracellular part as well. Toxic effects of the gold(III) complexes were evaluated in vitro by following lactate dehydrogenase activity in rat brain synaptosomes and incidence of micronuclei and cytokinesis-block proliferation index in cultivated human lymphocytes. All investigated complexes turned out to induce cytogenetic damage consisting of a significant decrease in cell proliferation and an increase in micronuclei in a dose-dependent manner. On the other hand, lactate dehydrogenase activity, an indicator of membrane integrity/viability, was not affected by Auoxo6 and Au(2)bipyC, while Au(2)phen slightly modified its activity.",
journal = "Journal of Biological Inorganic Chemistry",
title = "The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach",
volume = "22",
number = "6",
pages = "819-832",
doi = "10.1007/s00775-017-1460-5"
}

Bondžić, A. M., Čolović, M. B., Janjić, G. V., Zarić, B., Petrović, S., Krstić, D. Z., Marzo, T., Messori, L.,& Vasić, V. M.. (2017). The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach. in Journal of Biological Inorganic Chemistry, 22(6), 819-832.
https://doi.org/10.1007/s00775-017-1460-5

Bondžić AM, Čolović MB, Janjić GV, Zarić B, Petrović S, Krstić DZ, Marzo T, Messori L, Vasić VM. The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach. in Journal of Biological Inorganic Chemistry. 2017;22(6):819-832.
doi:10.1007/s00775-017-1460-5 .

Bondžić, Aleksandra M., Čolović, Mirjana B., Janjić, Goran V., Zarić, Božidarka, Petrović, Sandra, Krstić, Danijela Z., Marzo, Tiziano, Messori, Luigi, Vasić, Vesna M., "The influence of oxo-bridged binuclear gold(III) complexes on Na/K-ATPase activity: a joint experimental and theoretical approach" in Journal of Biological Inorganic Chemistry, 22, no. 6 (2017):819-832,
https://doi.org/10.1007/s00775-017-1460-5 . .

TY  - JOUR
AU  - Lazarević-Pašti, Tamara
AU  - Leskovac, Andreja
AU  - Momić, Tatjana
AU  - Petrović, Sandra
AU  - Vasić, Vesna M.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1796
AB  - Background: Acetylcholinesterase (AChE) is involved in the termination of impulse transmission by rapid hydrolysis of the neurotransmitter acetylcholine in numerous cholinergic pathways in the central and peripheral nervous systems. The enzyme inactivation leads to acetylcholine accumulation, hyperstimulation of nicotinic and muscarinic receptors, and disrupted neurotransmission. Hence, acetylcholinesterase inhibitors, interacting with the enzyme as their primary target, are applied as relevant drugs for different neurodegenerative diseases (such as Alzheimers and Parkinsons) as well as toxins. At the same time, there are increasing evidence that in non-neuronal context, AChE is involved in the regulation of cell proliferation, differentiation, apoptosis and cell-cell interaction. An irregular expression of AChE has been found in different types of tumors, suggesting the involvement of AChE in the regulation of tumor development. Having all this in mind, there is a possibility that some AChE inhibitors could be used as anti-cancer agents. Objective: This contribution will discuss a broad range of possible application of different AChE inhibitors as drugs, from well-known anti-Alzheimers disease drugs to their use in cancer treatment in future. Emphasis will be put on various known AChE inhibitors classes, whose application as drugs could be controversy, as well as on newly investigated natural products, which can also modulate AChE activity. Conclusion: It is not clear a patient treated for neurodegenerative condition prone to increased risk for some types of cancer and vice versa. This is necessary to keep in mind during rational drug design process for all therapies, which are based on AChE as a target molecule.
T2  - Current Medicinal Chemistry
T1  - Modulators of Acetylcholinesterase Activity: From Alzheimers Disease to Anti-Cancer Drugs
VL  - 24
IS  - 30
SP  - 3283
EP  - 3309
DO  - 10.2174/0929867324666170705123509
ER  - 

@article{
author = "Lazarević-Pašti, Tamara and Leskovac, Andreja and Momić, Tatjana and Petrović, Sandra and Vasić, Vesna M.",
year = "2017",
abstract = "Background: Acetylcholinesterase (AChE) is involved in the termination of impulse transmission by rapid hydrolysis of the neurotransmitter acetylcholine in numerous cholinergic pathways in the central and peripheral nervous systems. The enzyme inactivation leads to acetylcholine accumulation, hyperstimulation of nicotinic and muscarinic receptors, and disrupted neurotransmission. Hence, acetylcholinesterase inhibitors, interacting with the enzyme as their primary target, are applied as relevant drugs for different neurodegenerative diseases (such as Alzheimers and Parkinsons) as well as toxins. At the same time, there are increasing evidence that in non-neuronal context, AChE is involved in the regulation of cell proliferation, differentiation, apoptosis and cell-cell interaction. An irregular expression of AChE has been found in different types of tumors, suggesting the involvement of AChE in the regulation of tumor development. Having all this in mind, there is a possibility that some AChE inhibitors could be used as anti-cancer agents. Objective: This contribution will discuss a broad range of possible application of different AChE inhibitors as drugs, from well-known anti-Alzheimers disease drugs to their use in cancer treatment in future. Emphasis will be put on various known AChE inhibitors classes, whose application as drugs could be controversy, as well as on newly investigated natural products, which can also modulate AChE activity. Conclusion: It is not clear a patient treated for neurodegenerative condition prone to increased risk for some types of cancer and vice versa. This is necessary to keep in mind during rational drug design process for all therapies, which are based on AChE as a target molecule.",
journal = "Current Medicinal Chemistry",
title = "Modulators of Acetylcholinesterase Activity: From Alzheimers Disease to Anti-Cancer Drugs",
volume = "24",
number = "30",
pages = "3283-3309",
doi = "10.2174/0929867324666170705123509"
}

Lazarević-Pašti, T., Leskovac, A., Momić, T., Petrović, S.,& Vasić, V. M.. (2017). Modulators of Acetylcholinesterase Activity: From Alzheimers Disease to Anti-Cancer Drugs. in Current Medicinal Chemistry, 24(30), 3283-3309.
https://doi.org/10.2174/0929867324666170705123509

Lazarević-Pašti T, Leskovac A, Momić T, Petrović S, Vasić VM. Modulators of Acetylcholinesterase Activity: From Alzheimers Disease to Anti-Cancer Drugs. in Current Medicinal Chemistry. 2017;24(30):3283-3309.
doi:10.2174/0929867324666170705123509 .

Lazarević-Pašti, Tamara, Leskovac, Andreja, Momić, Tatjana, Petrović, Sandra, Vasić, Vesna M., "Modulators of Acetylcholinesterase Activity: From Alzheimers Disease to Anti-Cancer Drugs" in Current Medicinal Chemistry, 24, no. 30 (2017):3283-3309,
https://doi.org/10.2174/0929867324666170705123509 . .

TY  - JOUR
AU  - Petrović, Sandra
AU  - Vasić, Vesna M.
AU  - Mitrović, Tatjana
AU  - Lazović, Saša
AU  - Leskovac, Andreja
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1506
AB  - Undecylprodigiosin pigment (UPP) is reported to display cytotoxic activity towards various types of tumours. Nevertheless, its efficacy in modifying the cellular response to ionising radiation is still unknown. In this study, the radiomodulating effects of UPP were investigated. The effects of UPP were assessed in vitro by treating cultures of human peripheral blood with UPP and ionising radiation using two treatment regimens, the UPP pre-irradiation treatment and UPP post-irradiation treatment. The activity of UPP was investigated evaluating its effects on the radiation-induced micronuclei formation, cell proliferation, and induction of apoptosis. The redox modulating effects of UPP were examined measuring the catalase activity and the level of malondialdehyde, as a measure of oxidative stress. The results showed that UPP effects on cellular response to ionising radiation depend on its concentration and the timing of its administration. At low concentration, the UPP displayed radioprotective effects in. gamma-irradiated human lymphocytes while at higher concentrations, it acted as a radiosensitiser enhancing either mitotic catastrophe or apoptosis depending on the treatment regimen. The UPP modified redox processes in cells, particularly when it was employed prior to gamma-irradiation. Our data highlight the importance of further research of the potential of UPP to sensitize tumour cells to radiation therapy by inhibiting pathways that lead to treatment resistance.
T2  - Arhiv za higijenu rada i toksikologiju
T1  - The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro
VL  - 68
IS  - 1
SP  - 1
EP  - 8
DO  - 10.1515/aiht-2017-68-2897
ER  - 

@article{
author = "Petrović, Sandra and Vasić, Vesna M. and Mitrović, Tatjana and Lazović, Saša and Leskovac, Andreja",
year = "2017",
abstract = "Undecylprodigiosin pigment (UPP) is reported to display cytotoxic activity towards various types of tumours. Nevertheless, its efficacy in modifying the cellular response to ionising radiation is still unknown. In this study, the radiomodulating effects of UPP were investigated. The effects of UPP were assessed in vitro by treating cultures of human peripheral blood with UPP and ionising radiation using two treatment regimens, the UPP pre-irradiation treatment and UPP post-irradiation treatment. The activity of UPP was investigated evaluating its effects on the radiation-induced micronuclei formation, cell proliferation, and induction of apoptosis. The redox modulating effects of UPP were examined measuring the catalase activity and the level of malondialdehyde, as a measure of oxidative stress. The results showed that UPP effects on cellular response to ionising radiation depend on its concentration and the timing of its administration. At low concentration, the UPP displayed radioprotective effects in. gamma-irradiated human lymphocytes while at higher concentrations, it acted as a radiosensitiser enhancing either mitotic catastrophe or apoptosis depending on the treatment regimen. The UPP modified redox processes in cells, particularly when it was employed prior to gamma-irradiation. Our data highlight the importance of further research of the potential of UPP to sensitize tumour cells to radiation therapy by inhibiting pathways that lead to treatment resistance.",
journal = "Arhiv za higijenu rada i toksikologiju",
title = "The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro",
volume = "68",
number = "1",
pages = "1-8",
doi = "10.1515/aiht-2017-68-2897"
}

Petrović, S., Vasić, V. M., Mitrović, T., Lazović, S.,& Leskovac, A.. (2017). The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro. in Arhiv za higijenu rada i toksikologiju, 68(1), 1-8.
https://doi.org/10.1515/aiht-2017-68-2897

Petrović S, Vasić VM, Mitrović T, Lazović S, Leskovac A. The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro. in Arhiv za higijenu rada i toksikologiju. 2017;68(1):1-8.
doi:10.1515/aiht-2017-68-2897 .

Petrović, Sandra, Vasić, Vesna M., Mitrović, Tatjana, Lazović, Saša, Leskovac, Andreja, "The impact of concentration and administration time on the radiomodulating properties of undecylprodigiosin in vitro" in Arhiv za higijenu rada i toksikologiju, 68, no. 1 (2017):1-8,
https://doi.org/10.1515/aiht-2017-68-2897 . .

TY  - JOUR
AU  - Lazović, Saša
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Senerović, Lidija
AU  - Krivokapić, Nevena
AU  - Mitrović, Tatjana
AU  - Božović, Nikola
AU  - Vasić, Vesna M.
AU  - Nikodinović-Runić, Jasmina
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1370
AB  - It is known that some bacterial species are more resilient to different kinds of irradiation due to the naturally developed protective mechanisms and compounds such as pigments. On the other hand, reasoned tissue engineering using plasma remains a critical task and requires very precise control of plasma parameters in order to mitigate its potential detrimental effects. Here we isolated a natural protective agent, microbially produced undecylprodigiosin ((52)-4-methoxy-5-[(5-undecy1-1H-pyrrol2-yl)methylenel-1H,5H-2,2-bipyrrole), and investigated its effects on human blood cells independently and in combination with plasma. Two apprOaches were applied; the first, undecylprodigiosin (UP pigment) was added to the blood cultures, which then were exposed to plasma (pre-treatment); and the second- the blood cultures were exposed to plasma and then treated with pigment (post-treatment). The interactions of plasma and UP pigment with blood cells were investigated by conducting a series of biological tests providing the information regarding their genotoxicity, cytotoxicity and redox modulating activities. The exposure of cells to plasma induced oxidative stress as well as certain genotoxic and cytotoxic effects seen as elevated micronuclei incidence, decreased cell proliferation and enhanced apoptosis. In blood cultures treated with UP pigment alone, we found that both cytotoxic and protective effects could be induced depending on the concentration used. The highest UP pigment concentration increased lipid peroxidation and the incidence of micronuclei by more than 70% with maximal suppression of cell proliferation. On the contrary, we found that the lowest UP pigment concentration displayed protective effects. In combined treatments with plasma and UP pigment, we found that UP pigment could provide spatial shielding to plasma exposure. In the pre-treatment approach, the incidence of micronuclei was reduced by 35.52% compared to control while malondialdehyde level decreased by 36% indicating a significant mitigation of membrane damage induced by plasma. These results open perspectives for utilizing UP pigment for protection against overexposures in the field of plasma medicine. (C) 2016 Elsevier GmbH. All rights reserved.
T2  - Experimental and Toxicologic Pathology
T1  - Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro
VL  - 69
IS  - 1
SP  - 55
EP  - 62
DO  - 10.1016/j.etp.2016.11.003
ER  - 

@article{
author = "Lazović, Saša and Leskovac, Andreja and Petrović, Sandra and Senerović, Lidija and Krivokapić, Nevena and Mitrović, Tatjana and Božović, Nikola and Vasić, Vesna M. and Nikodinović-Runić, Jasmina",
year = "2017",
abstract = "It is known that some bacterial species are more resilient to different kinds of irradiation due to the naturally developed protective mechanisms and compounds such as pigments. On the other hand, reasoned tissue engineering using plasma remains a critical task and requires very precise control of plasma parameters in order to mitigate its potential detrimental effects. Here we isolated a natural protective agent, microbially produced undecylprodigiosin ((52)-4-methoxy-5-[(5-undecy1-1H-pyrrol2-yl)methylenel-1H,5H-2,2-bipyrrole), and investigated its effects on human blood cells independently and in combination with plasma. Two apprOaches were applied; the first, undecylprodigiosin (UP pigment) was added to the blood cultures, which then were exposed to plasma (pre-treatment); and the second- the blood cultures were exposed to plasma and then treated with pigment (post-treatment). The interactions of plasma and UP pigment with blood cells were investigated by conducting a series of biological tests providing the information regarding their genotoxicity, cytotoxicity and redox modulating activities. The exposure of cells to plasma induced oxidative stress as well as certain genotoxic and cytotoxic effects seen as elevated micronuclei incidence, decreased cell proliferation and enhanced apoptosis. In blood cultures treated with UP pigment alone, we found that both cytotoxic and protective effects could be induced depending on the concentration used. The highest UP pigment concentration increased lipid peroxidation and the incidence of micronuclei by more than 70% with maximal suppression of cell proliferation. On the contrary, we found that the lowest UP pigment concentration displayed protective effects. In combined treatments with plasma and UP pigment, we found that UP pigment could provide spatial shielding to plasma exposure. In the pre-treatment approach, the incidence of micronuclei was reduced by 35.52% compared to control while malondialdehyde level decreased by 36% indicating a significant mitigation of membrane damage induced by plasma. These results open perspectives for utilizing UP pigment for protection against overexposures in the field of plasma medicine. (C) 2016 Elsevier GmbH. All rights reserved.",
journal = "Experimental and Toxicologic Pathology",
title = "Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro",
volume = "69",
number = "1",
pages = "55-62",
doi = "10.1016/j.etp.2016.11.003"
}

Lazović, S., Leskovac, A., Petrović, S., Senerović, L., Krivokapić, N., Mitrović, T., Božović, N., Vasić, V. M.,& Nikodinović-Runić, J.. (2017). Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro. in Experimental and Toxicologic Pathology, 69(1), 55-62.
https://doi.org/10.1016/j.etp.2016.11.003

Lazović S, Leskovac A, Petrović S, Senerović L, Krivokapić N, Mitrović T, Božović N, Vasić VM, Nikodinović-Runić J. Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro. in Experimental and Toxicologic Pathology. 2017;69(1):55-62.
doi:10.1016/j.etp.2016.11.003 .

Lazović, Saša, Leskovac, Andreja, Petrović, Sandra, Senerović, Lidija, Krivokapić, Nevena, Mitrović, Tatjana, Božović, Nikola, Vasić, Vesna M., Nikodinović-Runić, Jasmina, "Biological effects of bacterial pigment undecylprodigiosin on human blood cells treated with atmospheric gas plasma in vitro" in Experimental and Toxicologic Pathology, 69, no. 1 (2017):55-62,
https://doi.org/10.1016/j.etp.2016.11.003 . .