TY  - JOUR
AU  - Figueroa, Fabian A.
AU  - Abdala-Díaz, Roberto T.
AU  - Pérez, Claudia
AU  - Casas-Arrojo, Virginia
AU  - Nešić, Aleksandra
AU  - Tapia, Cecilia
AU  - Durán, Carla
AU  - Valdes, Oscar
AU  - Parra, Carolina
AU  - Bravo-Arrepol, Gastón
AU  - Soto, Luis
AU  - Becerra, José
AU  - Cabrera-Barjas, Gustavo
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10388
AB  - Codium bernabei is a green alga that grows on Chilean coasts. The composition of its structural polysaccharides is still unknown. Hence, the aim of this work is to isolate and characterize the hot water extracted polysaccharide fractions. For this purpose, the water extracts were further precipitated in alcohol (TPs) and acid media (APs), respectively. Both fractions were characterized using different physicochemical techniques such as GC-MS, GPC, FTIR, TGA, and SEM. It is confirmed that the extracted fractions are mainly made of sulfated galactan unit, with a degree of sulfation of 19.3% (TPs) and 17.4% (ATs) and a protein content of 3.5% in APs and 15.6% in TPs. Other neutral sugars such as xylose, glucose, galactose, fucose, mannose, and arabinose were found in a molar ratio (0.05:0.6:1.0:0.02:0.14:0.11) for TPs and (0.05:0.31:1.0:0.03:0.1:0.13) for ATs. The molecular weight of the polysaccharide samples was lower than 20 kDa. Both polysaccharides were thermally stable (Tonset > 190 °C) and showed antioxidant activity according to the ABTS•+ and DPPH tests, where TPs fractions had higher scavenging activity (35%) compared to the APs fractions. The PT and APTTS assays were used to measure the anticoagulant activity of the polysaccharide fractions. In general, the PT activity of the TPs and APs was not different from normal plasma values. The exception was the TPs treatment at 1000 µg mL−1 concentration. The APTTS test revealed that clotting time for both polysaccharides was prolonged regarding normal values at 1000 µg mL−1. Finally, the antitumor test in colorectal carcinoma (HTC-116) cell line, breast cancer (MCF-7) and human leukemia (HL-60) cell lines showed the cytotoxic effect of TPs and APs. Those results suggest the potential biotechnological application of sulfate galactan polysaccharides isolated from a Chilean marine resource.
T2  - Marine Drugs
T1  - Sulfated Polysaccharide Extracted from the Green Algae Codium bernabei: Physicochemical Characterization and Antioxidant, Anticoagulant and Antitumor Activity
VL  - 20
IS  - 7
SP  - 458
DO  - 10.3390/md20070458
ER  - 

@article{
author = "Figueroa, Fabian A. and Abdala-Díaz, Roberto T. and Pérez, Claudia and Casas-Arrojo, Virginia and Nešić, Aleksandra and Tapia, Cecilia and Durán, Carla and Valdes, Oscar and Parra, Carolina and Bravo-Arrepol, Gastón and Soto, Luis and Becerra, José and Cabrera-Barjas, Gustavo",
year = "2022",
abstract = "Codium bernabei is a green alga that grows on Chilean coasts. The composition of its structural polysaccharides is still unknown. Hence, the aim of this work is to isolate and characterize the hot water extracted polysaccharide fractions. For this purpose, the water extracts were further precipitated in alcohol (TPs) and acid media (APs), respectively. Both fractions were characterized using different physicochemical techniques such as GC-MS, GPC, FTIR, TGA, and SEM. It is confirmed that the extracted fractions are mainly made of sulfated galactan unit, with a degree of sulfation of 19.3% (TPs) and 17.4% (ATs) and a protein content of 3.5% in APs and 15.6% in TPs. Other neutral sugars such as xylose, glucose, galactose, fucose, mannose, and arabinose were found in a molar ratio (0.05:0.6:1.0:0.02:0.14:0.11) for TPs and (0.05:0.31:1.0:0.03:0.1:0.13) for ATs. The molecular weight of the polysaccharide samples was lower than 20 kDa. Both polysaccharides were thermally stable (Tonset > 190 °C) and showed antioxidant activity according to the ABTS•+ and DPPH tests, where TPs fractions had higher scavenging activity (35%) compared to the APs fractions. The PT and APTTS assays were used to measure the anticoagulant activity of the polysaccharide fractions. In general, the PT activity of the TPs and APs was not different from normal plasma values. The exception was the TPs treatment at 1000 µg mL−1 concentration. The APTTS test revealed that clotting time for both polysaccharides was prolonged regarding normal values at 1000 µg mL−1. Finally, the antitumor test in colorectal carcinoma (HTC-116) cell line, breast cancer (MCF-7) and human leukemia (HL-60) cell lines showed the cytotoxic effect of TPs and APs. Those results suggest the potential biotechnological application of sulfate galactan polysaccharides isolated from a Chilean marine resource.",
journal = "Marine Drugs",
title = "Sulfated Polysaccharide Extracted from the Green Algae Codium bernabei: Physicochemical Characterization and Antioxidant, Anticoagulant and Antitumor Activity",
volume = "20",
number = "7",
pages = "458",
doi = "10.3390/md20070458"
}

Figueroa, F. A., Abdala-Díaz, R. T., Pérez, C., Casas-Arrojo, V., Nešić, A., Tapia, C., Durán, C., Valdes, O., Parra, C., Bravo-Arrepol, G., Soto, L., Becerra, J.,& Cabrera-Barjas, G.. (2022). Sulfated Polysaccharide Extracted from the Green Algae Codium bernabei: Physicochemical Characterization and Antioxidant, Anticoagulant and Antitumor Activity. in Marine Drugs, 20(7), 458.
https://doi.org/10.3390/md20070458

Figueroa FA, Abdala-Díaz RT, Pérez C, Casas-Arrojo V, Nešić A, Tapia C, Durán C, Valdes O, Parra C, Bravo-Arrepol G, Soto L, Becerra J, Cabrera-Barjas G. Sulfated Polysaccharide Extracted from the Green Algae Codium bernabei: Physicochemical Characterization and Antioxidant, Anticoagulant and Antitumor Activity. in Marine Drugs. 2022;20(7):458.
doi:10.3390/md20070458 .

Figueroa, Fabian A., Abdala-Díaz, Roberto T., Pérez, Claudia, Casas-Arrojo, Virginia, Nešić, Aleksandra, Tapia, Cecilia, Durán, Carla, Valdes, Oscar, Parra, Carolina, Bravo-Arrepol, Gastón, Soto, Luis, Becerra, José, Cabrera-Barjas, Gustavo, "Sulfated Polysaccharide Extracted from the Green Algae Codium bernabei: Physicochemical Characterization and Antioxidant, Anticoagulant and Antitumor Activity" in Marine Drugs, 20, no. 7 (2022):458,
https://doi.org/10.3390/md20070458 . .