TY  - JOUR
AU  - Kolaković, Ana
AU  - Bundalo, Maja
AU  - Đurić, Tamara
AU  - Končar, Igor
AU  - Stanković, Aleksandra
AU  - Živković, Maja
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13103
AB  - Background/Aim. The renin-angiotensin system (RAS) is linked to the development of atherosclerosis (As), including its initiation and progression. Besides the well-known angio-tensin-converting enzyme (ACE), two newer RAS family members are related to vascular remodeling - ACE2 as a homolog of ACE and collectrin [transmembrane protein 27 (TMEM27)] as a homolog of ACE2. Up to now, a limited number of studies have examined the expression of these RAS components in advanced carotid plaque (CP) tissue based on the sex of the patients and plaque phenotypes (PPs). There are two ultrasonographically defined PPs - the hypoechogenic plaque (HoP) and the hyperechogenic plaque (HerP) phenotype. The aim of the study was to investigate whether there was a correlation between the expression of RAS components in the CP and the sex and PPs of patients. Methods. We examined 74 patients with advanced CP who underwent carotid endarterectomy. The intraplaque expression of RAS components was determined with the real-time polymerase chain reaction, using the TaqMan® gene expression assays and Western blot. A two-way ANOVA followed by a post-hoc Tukey test was performed for the statistical analysis of results. Results. No interaction was recorded between the sex of the patients and PPs in influencing the relative expression of ACE and TMEM27 messenger RNA (mRNA) (p > 0.05). In 56.06% of plaque samples, no expression of ACE2 mRNA was detected. Among the plaques where ACE2 mRNA expression was detected, its expression level was higher in females with the HoP phenotype compared to females with the HerP phenotype (p < 0.001). In patients with the HoP phenotype, females had higher expression of ACE2 mRNA than males (p < 0.05). In the male study group, ACE protein levels were significantly lower in the HoP phenotype compared to the HerP phenotype (p < 0.001). Fe-males with the HoP phenotype had significantly higher ACE protein levels than males with the HoP phenotype (p < 0.0001). Conclusion. Our results revealed alterations in the expression levels of ACE and ACE2, at the mRNA and protein levels, in advanced carotid As. These alterations are impacted by sex and PP and may indicate a switch from the balanced RAS/ACE/ACE2 axis in the healthy blood vessel to the unbalanced axis in vascular remodeling due to As.
AB  - Renin-angiotenzin sistem (RAS) povezan je sa
razvojem ateroskleroze (As), uključujući njen nastanak i
progresiju. Pored dobro poznatog angiotenzinkonvertujućeg enzima (angiotensin-converting enzyme – ACE),
dva nova člana RAS familije povezana su sa
remodelovanjem zidova krvnih sudova – ACE2 kao
homolog ACE i kolektrin [transmembrane protein 27
(TMEM27)] kao homolog ACE2. Do sada je mali broj
studija ispitivao ekspresiju komponenti RAS sistema u tkivu
uznapredovalog karotidnog plaka (KP) u odnosu na pol
bolesnika i fenotip plaka (FP). Postoje dva tipa KP
definisana primenom ultrazvuka – fenotip hipoehogenog
plaka (HoP) i fenotip hiperehogenog plaka (HerP). Cilj rada
bio je da se ispita da li postoji korelacija između ekspresije
komponenti RAS u KP i pola i FP bolesnika. Metode.
Ispitano je 74 bolesnika sa uznapredovalim KP koji su bili
podvrgnuti operativnoj proceduri karotidne
endarterektomije. Ekspresija komponenti RAS sistema u
tkivu plaka utvrđena je lančanom reakcijom polimeraze u
realnom vremenu (real-time polymerase chain reaction)primenom eseja TaqMan® tehnologije i metode Western blota. Dvosmerna analiza varijanse i Tukey post-hoc test korišćen su za statističku obradu rezultata. Rezultati. Nije utvrđena
interakcija FP i pola bolesnika u uticaju na relativnu
ekspresiju informacione RNK (iRNK) za ACE i TMEM27
(p > 0,05). U 56,06% uzoraka plaka nije detektovana
ekspresija iRNK za ACE2. U plakovima u kojima je
detektovana ekspresija iRNK za ACE2, njen nivo bio je viši
kod žena sa HoP u poređenju sa ženama sa HerP (p <
0,001). U grupi bolesnika sa fenotipom HoP, žene su imale
viši nivo iRNK za ACE2 nego muškarci (p < 0,05). U grupi
muškaraca, nivoi ekspresije ACE proteina bili suznačajno
niži u fenotipu HoP u poređenju sa HerP (p < 0,001). Žene
sa fenotipom HoP imale su značajno viši nivo ACE
proteina u poređenju sa muškarcima sa HoP (p < 0,0001).
Zaključak. Naši rezultati pokazali su da postoje promene
nivoa ekspresije ACE i ACE2, na nivou proteina i iRNK u
uznapredovaloj karotidnoj As. Te promene zavise od pola i
FP i mogu ukazivati na to da balans ose RAS/ACE/ACE2
koji postoji u zdravom krvnom sudu postaje poremećen
tokom remodelovanja zida krvnog suda usled As.
T2  - Vojnosanitetski pregled
T1  - The expression of renin-angiotensin system components in human carotid plaque
T1  - Ekspresija komponenti renin-angiotenzin sistema u humanom karotidnom plaku
DO  - 10.2298/VSP221028014K
ER  - 

@article{
author = "Kolaković, Ana and Bundalo, Maja and Đurić, Tamara and Končar, Igor and Stanković, Aleksandra and Živković, Maja",
year = "2024",
abstract = "Background/Aim. The renin-angiotensin system (RAS) is linked to the development of atherosclerosis (As), including its initiation and progression. Besides the well-known angio-tensin-converting enzyme (ACE), two newer RAS family members are related to vascular remodeling - ACE2 as a homolog of ACE and collectrin [transmembrane protein 27 (TMEM27)] as a homolog of ACE2. Up to now, a limited number of studies have examined the expression of these RAS components in advanced carotid plaque (CP) tissue based on the sex of the patients and plaque phenotypes (PPs). There are two ultrasonographically defined PPs - the hypoechogenic plaque (HoP) and the hyperechogenic plaque (HerP) phenotype. The aim of the study was to investigate whether there was a correlation between the expression of RAS components in the CP and the sex and PPs of patients. Methods. We examined 74 patients with advanced CP who underwent carotid endarterectomy. The intraplaque expression of RAS components was determined with the real-time polymerase chain reaction, using the TaqMan® gene expression assays and Western blot. A two-way ANOVA followed by a post-hoc Tukey test was performed for the statistical analysis of results. Results. No interaction was recorded between the sex of the patients and PPs in influencing the relative expression of ACE and TMEM27 messenger RNA (mRNA) (p > 0.05). In 56.06% of plaque samples, no expression of ACE2 mRNA was detected. Among the plaques where ACE2 mRNA expression was detected, its expression level was higher in females with the HoP phenotype compared to females with the HerP phenotype (p < 0.001). In patients with the HoP phenotype, females had higher expression of ACE2 mRNA than males (p < 0.05). In the male study group, ACE protein levels were significantly lower in the HoP phenotype compared to the HerP phenotype (p < 0.001). Fe-males with the HoP phenotype had significantly higher ACE protein levels than males with the HoP phenotype (p < 0.0001). Conclusion. Our results revealed alterations in the expression levels of ACE and ACE2, at the mRNA and protein levels, in advanced carotid As. These alterations are impacted by sex and PP and may indicate a switch from the balanced RAS/ACE/ACE2 axis in the healthy blood vessel to the unbalanced axis in vascular remodeling due to As., Renin-angiotenzin sistem (RAS) povezan je sa
razvojem ateroskleroze (As), uključujući njen nastanak i
progresiju. Pored dobro poznatog angiotenzinkonvertujućeg enzima (angiotensin-converting enzyme – ACE),
dva nova člana RAS familije povezana su sa
remodelovanjem zidova krvnih sudova – ACE2 kao
homolog ACE i kolektrin [transmembrane protein 27
(TMEM27)] kao homolog ACE2. Do sada je mali broj
studija ispitivao ekspresiju komponenti RAS sistema u tkivu
uznapredovalog karotidnog plaka (KP) u odnosu na pol
bolesnika i fenotip plaka (FP). Postoje dva tipa KP
definisana primenom ultrazvuka – fenotip hipoehogenog
plaka (HoP) i fenotip hiperehogenog plaka (HerP). Cilj rada
bio je da se ispita da li postoji korelacija između ekspresije
komponenti RAS u KP i pola i FP bolesnika. Metode.
Ispitano je 74 bolesnika sa uznapredovalim KP koji su bili
podvrgnuti operativnoj proceduri karotidne
endarterektomije. Ekspresija komponenti RAS sistema u
tkivu plaka utvrđena je lančanom reakcijom polimeraze u
realnom vremenu (real-time polymerase chain reaction)primenom eseja TaqMan® tehnologije i metode Western blota. Dvosmerna analiza varijanse i Tukey post-hoc test korišćen su za statističku obradu rezultata. Rezultati. Nije utvrđena
interakcija FP i pola bolesnika u uticaju na relativnu
ekspresiju informacione RNK (iRNK) za ACE i TMEM27
(p > 0,05). U 56,06% uzoraka plaka nije detektovana
ekspresija iRNK za ACE2. U plakovima u kojima je
detektovana ekspresija iRNK za ACE2, njen nivo bio je viši
kod žena sa HoP u poređenju sa ženama sa HerP (p <
0,001). U grupi bolesnika sa fenotipom HoP, žene su imale
viši nivo iRNK za ACE2 nego muškarci (p < 0,05). U grupi
muškaraca, nivoi ekspresije ACE proteina bili suznačajno
niži u fenotipu HoP u poređenju sa HerP (p < 0,001). Žene
sa fenotipom HoP imale su značajno viši nivo ACE
proteina u poređenju sa muškarcima sa HoP (p < 0,0001).
Zaključak. Naši rezultati pokazali su da postoje promene
nivoa ekspresije ACE i ACE2, na nivou proteina i iRNK u
uznapredovaloj karotidnoj As. Te promene zavise od pola i
FP i mogu ukazivati na to da balans ose RAS/ACE/ACE2
koji postoji u zdravom krvnom sudu postaje poremećen
tokom remodelovanja zida krvnog suda usled As.",
journal = "Vojnosanitetski pregled",
title = "The expression of renin-angiotensin system components in human carotid plaque, Ekspresija komponenti renin-angiotenzin sistema u humanom karotidnom plaku",
doi = "10.2298/VSP221028014K"
}

Kolaković, A., Bundalo, M., Đurić, T., Končar, I., Stanković, A.,& Živković, M.. (2024). The expression of renin-angiotensin system components in human carotid plaque. in Vojnosanitetski pregled.
https://doi.org/10.2298/VSP221028014K

Kolaković A, Bundalo M, Đurić T, Končar I, Stanković A, Živković M. The expression of renin-angiotensin system components in human carotid plaque. in Vojnosanitetski pregled. 2024;.
doi:10.2298/VSP221028014K .

Kolaković, Ana, Bundalo, Maja, Đurić, Tamara, Končar, Igor, Stanković, Aleksandra, Živković, Maja, "The expression of renin-angiotensin system components in human carotid plaque" in Vojnosanitetski pregled (2024),
https://doi.org/10.2298/VSP221028014K . .

TY  - CONF
AU  - Kožik, Bojana
AU  - Todorović, Lidija
AU  - Božović, Ana
AU  - Kolaković, Ana
AU  - Vasiljević, Tijana
AU  - Đurić, Mladen
AU  - Đermanović, Aleksandar
AU  - Mandušić, Vesna
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13104
AB  - Introduction: Venous invasion has consistently been shown to be associated with poor prognosis in rectal carcinoma (RC), both when detected by pathology and radiology. Extramural venous invasion (EMVI) is characterized by the presence of tumor cells within veins outside the bowel wall and is strongly associated with poor survival and increased risk of local recurrence and distant metastases. Molecular basis of EMVI is still unexplored and genes that regulate tumor microenvironment interactions may have significant role in this process. ZEB1 is transcriptional factor that promote cancerogenesis by indirect regulation of epithelial-mesenchymal transition (EMT) process, while LOXL2 contributes to tumor invasion and metastasis due to its role in the stabilization of the extracellular matrix. Aim: This study aimed to compare the expression level of ZEB1 and LOXL2 genes in relation to EMVI status and other clinic-pathological parameters of RC patients. Methods: We conducted preliminary study on 21 untreated RC patients (9 EMVI+ and 11 EMVI- ) who underwent curative resection in 2016-2018 at Oncology Institute of Vojvodina. The presence of EMVI was assessed on standard hematoxylin and eosin-stained histolological sections of postoperative tumor specimen samples, from which RNA was isolated. Expression of ZEB1 and LOXL2 mRNA was measured using quantitative real-time PCR. Results: Comparative analysis revealed higher expression level of ZEB1 in EMVI positive samples and in patients in TNMIII stage, however the observed differences had no statistic significance (p=0.323 and p=0.197, respectively). Significant difference in LOXL2 expression according to the EMVI status was not detected (p=0.915), while we noted higher LOXL2 expression in late stages of disease, but without statistic significance (p=0.342). Relative expression of these two genes was not associated with metastases frequency and death outcome. Conclusion: Further analyses on larger number of samples with more potential molecular targets included are required and planed.
AB  - Uvod: Venska invazija je kontinuirano asocirana sa lošom prognozom kod obolelih od karcinoma rektuma (KR), bilo da je detektovana patološkim ili radiološkim metodama. Ekstramuralna venska invazija (EMVI) se karakteriše kao prisustvo tumorskih ćelija u venskim sudovima izvan zida debelog creva koje je značajno asocirano sa lošim preživljavanjem i povećanim rizikom za nastanak lokalnih recidiva i udaljenih metastaza. Molekularna osnova EMVI procesa nije dovoljno ispitana, a geni koji regulišu interakcije u tumorskoj mikrosredini mogu imati potencijalnu ulogu. ZEB1 je transkripcioni factor koji stimuliše kancerogenezu indirektnom regulacijom epitelnomezenhimske tranzicije (EMT), dok LOXL2 doprinosi procesu tumorske invazije ulogom u stabilizaciji ektracelularnog matriksa. Cilj: Cilj ove studije je uporediti relativnu ekspresije gena ZEB1 i LOXL2 u odnosu na EMVI status i druge kliničko-patološke parametre KR pacijenta. Metode: Ova preliminarna studija obuhvatila je 21 netretiranih KR pacijenata (9 EMVI+ i 11 EMVI-) koji su lečeni operativnim putem u periodu 2016-2018. god. u Institutu za onkologiju Vojvodina. Prisustvo EMVI je utvrđeno na standardno hematoksilinom i eozinom bojenim isečcima postoperativnog tumorskog tkiva iz kojih je izolovana RNK. Ekspresija ZEB1 i LOXL2 iRNA izmerena je kvantitativnom PCR metodom u realnom vremenu. Rezultati: Uporednom analizom uočena je povišena ekspresija ZEB1 kod EMVI+ uzoraka i kod obolelih u TNMIII stadijumu, ali uočene razlike nisu bile statistički značajne (p=0,323 i p=0,197, respektivno). Znčajna razlika u ekspresiji LOXL2 u odnosu na EMVI status nije detektovana (p=0,915), a zabeležena je i povećana ekspresija LOXL2 u kasnim stadijumima bolesti, ali bez statističke značajnosti (p=0,342). Relativna ekspresija ova dva gena nije značajno povezana sa pojavom metstaza i krajnjim ishodom bolesti. Zaključak: Dalje analize na većem broju uzoraka sa više uključenih molekularnih targeta u studiju su neophodne i planirane u budućnosti.
PB  - Belgrade : Serbian Medical Society Oncology Section
C3  - Anali kancerološke sekcije SLD : 60. Kancerološka nedelja : Knjiga apstrakata
T1  - Expression of ZEB1 and LOXL2 in rectal carcinoma and their correlation with extramural venous invasion (EMVI): preliminary study
T1  - Ekspresija ZEB1 i LOXL2 gena kod karcinoma rektuma i njihova korelacija sa ekstramuralnom venskom invazijom (EMVI): preliminarna studija
UR  - https://hdl.handle.net/21.15107/rcub_vinar_13104
ER  - 

@conference{
author = "Kožik, Bojana and Todorović, Lidija and Božović, Ana and Kolaković, Ana and Vasiljević, Tijana and Đurić, Mladen and Đermanović, Aleksandar and Mandušić, Vesna",
year = "2023",
abstract = "Introduction: Venous invasion has consistently been shown to be associated with poor prognosis in rectal carcinoma (RC), both when detected by pathology and radiology. Extramural venous invasion (EMVI) is characterized by the presence of tumor cells within veins outside the bowel wall and is strongly associated with poor survival and increased risk of local recurrence and distant metastases. Molecular basis of EMVI is still unexplored and genes that regulate tumor microenvironment interactions may have significant role in this process. ZEB1 is transcriptional factor that promote cancerogenesis by indirect regulation of epithelial-mesenchymal transition (EMT) process, while LOXL2 contributes to tumor invasion and metastasis due to its role in the stabilization of the extracellular matrix. Aim: This study aimed to compare the expression level of ZEB1 and LOXL2 genes in relation to EMVI status and other clinic-pathological parameters of RC patients. Methods: We conducted preliminary study on 21 untreated RC patients (9 EMVI+ and 11 EMVI- ) who underwent curative resection in 2016-2018 at Oncology Institute of Vojvodina. The presence of EMVI was assessed on standard hematoxylin and eosin-stained histolological sections of postoperative tumor specimen samples, from which RNA was isolated. Expression of ZEB1 and LOXL2 mRNA was measured using quantitative real-time PCR. Results: Comparative analysis revealed higher expression level of ZEB1 in EMVI positive samples and in patients in TNMIII stage, however the observed differences had no statistic significance (p=0.323 and p=0.197, respectively). Significant difference in LOXL2 expression according to the EMVI status was not detected (p=0.915), while we noted higher LOXL2 expression in late stages of disease, but without statistic significance (p=0.342). Relative expression of these two genes was not associated with metastases frequency and death outcome. Conclusion: Further analyses on larger number of samples with more potential molecular targets included are required and planed., Uvod: Venska invazija je kontinuirano asocirana sa lošom prognozom kod obolelih od karcinoma rektuma (KR), bilo da je detektovana patološkim ili radiološkim metodama. Ekstramuralna venska invazija (EMVI) se karakteriše kao prisustvo tumorskih ćelija u venskim sudovima izvan zida debelog creva koje je značajno asocirano sa lošim preživljavanjem i povećanim rizikom za nastanak lokalnih recidiva i udaljenih metastaza. Molekularna osnova EMVI procesa nije dovoljno ispitana, a geni koji regulišu interakcije u tumorskoj mikrosredini mogu imati potencijalnu ulogu. ZEB1 je transkripcioni factor koji stimuliše kancerogenezu indirektnom regulacijom epitelnomezenhimske tranzicije (EMT), dok LOXL2 doprinosi procesu tumorske invazije ulogom u stabilizaciji ektracelularnog matriksa. Cilj: Cilj ove studije je uporediti relativnu ekspresije gena ZEB1 i LOXL2 u odnosu na EMVI status i druge kliničko-patološke parametre KR pacijenta. Metode: Ova preliminarna studija obuhvatila je 21 netretiranih KR pacijenata (9 EMVI+ i 11 EMVI-) koji su lečeni operativnim putem u periodu 2016-2018. god. u Institutu za onkologiju Vojvodina. Prisustvo EMVI je utvrđeno na standardno hematoksilinom i eozinom bojenim isečcima postoperativnog tumorskog tkiva iz kojih je izolovana RNK. Ekspresija ZEB1 i LOXL2 iRNA izmerena je kvantitativnom PCR metodom u realnom vremenu. Rezultati: Uporednom analizom uočena je povišena ekspresija ZEB1 kod EMVI+ uzoraka i kod obolelih u TNMIII stadijumu, ali uočene razlike nisu bile statistički značajne (p=0,323 i p=0,197, respektivno). Znčajna razlika u ekspresiji LOXL2 u odnosu na EMVI status nije detektovana (p=0,915), a zabeležena je i povećana ekspresija LOXL2 u kasnim stadijumima bolesti, ali bez statističke značajnosti (p=0,342). Relativna ekspresija ova dva gena nije značajno povezana sa pojavom metstaza i krajnjim ishodom bolesti. Zaključak: Dalje analize na većem broju uzoraka sa više uključenih molekularnih targeta u studiju su neophodne i planirane u budućnosti.",
publisher = "Belgrade : Serbian Medical Society Oncology Section",
journal = "Anali kancerološke sekcije SLD : 60. Kancerološka nedelja : Knjiga apstrakata",
title = "Expression of ZEB1 and LOXL2 in rectal carcinoma and their correlation with extramural venous invasion (EMVI): preliminary study, Ekspresija ZEB1 i LOXL2 gena kod karcinoma rektuma i njihova korelacija sa ekstramuralnom venskom invazijom (EMVI): preliminarna studija",
url = "https://hdl.handle.net/21.15107/rcub_vinar_13104"
}

Kožik, B., Todorović, L., Božović, A., Kolaković, A., Vasiljević, T., Đurić, M., Đermanović, A.,& Mandušić, V.. (2023). Expression of ZEB1 and LOXL2 in rectal carcinoma and their correlation with extramural venous invasion (EMVI): preliminary study. in Anali kancerološke sekcije SLD : 60. Kancerološka nedelja : Knjiga apstrakata
Belgrade : Serbian Medical Society Oncology Section..
https://hdl.handle.net/21.15107/rcub_vinar_13104

Kožik B, Todorović L, Božović A, Kolaković A, Vasiljević T, Đurić M, Đermanović A, Mandušić V. Expression of ZEB1 and LOXL2 in rectal carcinoma and their correlation with extramural venous invasion (EMVI): preliminary study. in Anali kancerološke sekcije SLD : 60. Kancerološka nedelja : Knjiga apstrakata. 2023;.
https://hdl.handle.net/21.15107/rcub_vinar_13104 .

Kožik, Bojana, Todorović, Lidija, Božović, Ana, Kolaković, Ana, Vasiljević, Tijana, Đurić, Mladen, Đermanović, Aleksandar, Mandušić, Vesna, "Expression of ZEB1 and LOXL2 in rectal carcinoma and their correlation with extramural venous invasion (EMVI): preliminary study" in Anali kancerološke sekcije SLD : 60. Kancerološka nedelja : Knjiga apstrakata (2023),
https://hdl.handle.net/21.15107/rcub_vinar_13104 .

TY  - CONF
AU  - Kolaković, Ana
AU  - Đurić, Tamara
AU  - Živković, Maja
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12700
PB  - Kragujevac : Srpsko biološko društvo „Stevan Jakovljević“
C3  - Prva Konferencija Srpskog Biološkog Društva „Stevan Jakovljević“ : Program i izvodi saopštenja
T1  - Varijante u genima familije sirtuina: potencijal za buduća istraživanja u kardiovaskularnim bolestima
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12700
ER  - 

@conference{
author = "Kolaković, Ana and Đurić, Tamara and Živković, Maja",
year = "2023",
publisher = "Kragujevac : Srpsko biološko društvo „Stevan Jakovljević“",
journal = "Prva Konferencija Srpskog Biološkog Društva „Stevan Jakovljević“ : Program i izvodi saopštenja",
title = "Varijante u genima familije sirtuina: potencijal za buduća istraživanja u kardiovaskularnim bolestima",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12700"
}

Kolaković, A., Đurić, T.,& Živković, M.. (2023). Varijante u genima familije sirtuina: potencijal za buduća istraživanja u kardiovaskularnim bolestima. in Prva Konferencija Srpskog Biološkog Društva „Stevan Jakovljević“ : Program i izvodi saopštenja
Kragujevac : Srpsko biološko društvo „Stevan Jakovljević“..
https://hdl.handle.net/21.15107/rcub_vinar_12700

Kolaković A, Đurić T, Živković M. Varijante u genima familije sirtuina: potencijal za buduća istraživanja u kardiovaskularnim bolestima. in Prva Konferencija Srpskog Biološkog Društva „Stevan Jakovljević“ : Program i izvodi saopštenja. 2023;.
https://hdl.handle.net/21.15107/rcub_vinar_12700 .

Kolaković, Ana, Đurić, Tamara, Živković, Maja, "Varijante u genima familije sirtuina: potencijal za buduća istraživanja u kardiovaskularnim bolestima" in Prva Konferencija Srpskog Biološkog Društva „Stevan Jakovljević“ : Program i izvodi saopštenja (2023),
https://hdl.handle.net/21.15107/rcub_vinar_12700 .

TY  - JOUR
AU  - Živković, Maja
AU  - Bubić, Maja
AU  - Kolaković, Ana
AU  - Dekleva, Milica
AU  - Stanković, Goran
AU  - Stanković, Aleksandra
AU  - Đurić, Tamara
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9842
AB  - Glutathione S-transferases (GSTs) are the family of enzymes involved in the second line of defense against oxidative stress (OS). The lack of GSTT1/GSTM1 enzyme quantity or activity, due to the presence of homozygous deletion compromises antioxidative defense resulting in OS. OS is the critical mechanism in the pathophysiology of atherosclerosis, coronary artery disease, and myocardial infarction (MI). The increase in reactive oxygen species together with the process of apoptosis plays a role in left ventricular remodeling (LVR) after MI. The associations of GSTT1 and GSTM1 gene polymorphisms with the risk of MI are inconsistent. The aim was to analyze the association of GSTT1/GSTM1 null genotypes with first MI and LVR 8 months after the MI. The study involved 330 controls and 438 consecutive patients with symptoms and signs of first MI. The subgroup of 150 MI patients was prospectively followed up for 6 months. Evidence of maladaptive LVR was obtained by 2D Doppler echocardiography 3-5 days and 6 months after the MI. A multiplex polymerase chain reaction was used to detect the deletion in GSTT1 and GSTM1 genes. GSTM1 null genotype was significantly and independently associated with first MI (adjusted OR = 1.45 95% CI 1.03-2.03, p = 0.03). Association of double null genotypes with maladaptive LVR in patients 6 months after the first MI was no longer significant after adjustment for factors that differed significantly between patients with and without maladaptive LVR. This study demonstrated the association of GSTM1 null genotypes with the risk of MI in the Serbian population.
T2  - Free Radical Research
T1  - The association of glutathione S-transferase T1 and M1 deletions with myocardial infarction
VL  - 55
IS  - 3
SP  - 267
EP  - 274
DO  - 10.1080/10715762.2021.1931166
ER  - 

@article{
author = "Živković, Maja and Bubić, Maja and Kolaković, Ana and Dekleva, Milica and Stanković, Goran and Stanković, Aleksandra and Đurić, Tamara",
year = "2021",
abstract = "Glutathione S-transferases (GSTs) are the family of enzymes involved in the second line of defense against oxidative stress (OS). The lack of GSTT1/GSTM1 enzyme quantity or activity, due to the presence of homozygous deletion compromises antioxidative defense resulting in OS. OS is the critical mechanism in the pathophysiology of atherosclerosis, coronary artery disease, and myocardial infarction (MI). The increase in reactive oxygen species together with the process of apoptosis plays a role in left ventricular remodeling (LVR) after MI. The associations of GSTT1 and GSTM1 gene polymorphisms with the risk of MI are inconsistent. The aim was to analyze the association of GSTT1/GSTM1 null genotypes with first MI and LVR 8 months after the MI. The study involved 330 controls and 438 consecutive patients with symptoms and signs of first MI. The subgroup of 150 MI patients was prospectively followed up for 6 months. Evidence of maladaptive LVR was obtained by 2D Doppler echocardiography 3-5 days and 6 months after the MI. A multiplex polymerase chain reaction was used to detect the deletion in GSTT1 and GSTM1 genes. GSTM1 null genotype was significantly and independently associated with first MI (adjusted OR = 1.45 95% CI 1.03-2.03, p = 0.03). Association of double null genotypes with maladaptive LVR in patients 6 months after the first MI was no longer significant after adjustment for factors that differed significantly between patients with and without maladaptive LVR. This study demonstrated the association of GSTM1 null genotypes with the risk of MI in the Serbian population.",
journal = "Free Radical Research",
title = "The association of glutathione S-transferase T1 and M1 deletions with myocardial infarction",
volume = "55",
number = "3",
pages = "267-274",
doi = "10.1080/10715762.2021.1931166"
}

Živković, M., Bubić, M., Kolaković, A., Dekleva, M., Stanković, G., Stanković, A.,& Đurić, T.. (2021). The association of glutathione S-transferase T1 and M1 deletions with myocardial infarction. in Free Radical Research, 55(3), 267-274.
https://doi.org/10.1080/10715762.2021.1931166

Živković M, Bubić M, Kolaković A, Dekleva M, Stanković G, Stanković A, Đurić T. The association of glutathione S-transferase T1 and M1 deletions with myocardial infarction. in Free Radical Research. 2021;55(3):267-274.
doi:10.1080/10715762.2021.1931166 .

Živković, Maja, Bubić, Maja, Kolaković, Ana, Dekleva, Milica, Stanković, Goran, Stanković, Aleksandra, Đurić, Tamara, "The association of glutathione S-transferase T1 and M1 deletions with myocardial infarction" in Free Radical Research, 55, no. 3 (2021):267-274,
https://doi.org/10.1080/10715762.2021.1931166 . .

TY  - JOUR
AU  - Stojković, Ljiljana S.
AU  - Jovanović, Ivan G.
AU  - Živković, Maja
AU  - Zec, Manja
AU  - Đurić, Tamara
AU  - Životić, Ivan
AU  - Kuveljić, Jovana
AU  - Kolaković, Ana
AU  - Kolić, Ivana
AU  - Đorđević, Ana
AU  - Glibetić, Marija
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8683
AB  - Foods and food products that contain polyphenols are proposed to modulate risk of cardiovascular disease. The aim of this three-arm, crossover, randomized, double-blind, placebo-controlled intervention study was to examine the impact of Aronia melanocarpa juice (AMJ), high-polyphenol (AMJ treatment, 1.17 g/100 mL polyphenols) and low-polyphenol (dAMJ treatment, 0.29 g/100 mL polyphenols) dose, on the transcriptome in peripheral blood mononuclear cells (PBMC) of 19 subjects at cardiovascular risk. Transcriptome data were obtained by microarray. Bioinformatic functional annotation analysis was performed on both the whole transcriptome datasets and the differentially expressed genes (DEGs). Expression of selected DEGs was validated by RT-qPCR. Administration of AMJ and dAMJ treatments during the two consecutive four-week treatment periods had additive effects on PBMC transcriptome profiles, with the most pronounced and specific effect noticed for AMJ in the last treatment period (TP3) of the trial. Between the high-dose and low-dose treatments in TP3, there was a multitude of overlapping DEGs and DEG-enriched biological processes and pathways, which primarily included immunomodulation and regulation of cell proliferation/death. Increased expression of TNF, IL1B, IL8, RGS1, OSM, and DUSP2 in TP3 was confirmed by RT-qPCR. The results suggest the immunomodulatory effects of prolonged habitual consumption of polyphenol-rich aronia juice in individuals at cardiovascular risk.
T2  - Nutrients
T1  - The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk
VL  - 12
IS  - 5
SP  - 1484
DO  - 10.3390/nu12051484
ER  - 

@article{
author = "Stojković, Ljiljana S. and Jovanović, Ivan G. and Živković, Maja and Zec, Manja and Đurić, Tamara and Životić, Ivan and Kuveljić, Jovana and Kolaković, Ana and Kolić, Ivana and Đorđević, Ana and Glibetić, Marija and Alavantić, Dragan and Stanković, Aleksandra",
year = "2020",
abstract = "Foods and food products that contain polyphenols are proposed to modulate risk of cardiovascular disease. The aim of this three-arm, crossover, randomized, double-blind, placebo-controlled intervention study was to examine the impact of Aronia melanocarpa juice (AMJ), high-polyphenol (AMJ treatment, 1.17 g/100 mL polyphenols) and low-polyphenol (dAMJ treatment, 0.29 g/100 mL polyphenols) dose, on the transcriptome in peripheral blood mononuclear cells (PBMC) of 19 subjects at cardiovascular risk. Transcriptome data were obtained by microarray. Bioinformatic functional annotation analysis was performed on both the whole transcriptome datasets and the differentially expressed genes (DEGs). Expression of selected DEGs was validated by RT-qPCR. Administration of AMJ and dAMJ treatments during the two consecutive four-week treatment periods had additive effects on PBMC transcriptome profiles, with the most pronounced and specific effect noticed for AMJ in the last treatment period (TP3) of the trial. Between the high-dose and low-dose treatments in TP3, there was a multitude of overlapping DEGs and DEG-enriched biological processes and pathways, which primarily included immunomodulation and regulation of cell proliferation/death. Increased expression of TNF, IL1B, IL8, RGS1, OSM, and DUSP2 in TP3 was confirmed by RT-qPCR. The results suggest the immunomodulatory effects of prolonged habitual consumption of polyphenol-rich aronia juice in individuals at cardiovascular risk.",
journal = "Nutrients",
title = "The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk",
volume = "12",
number = "5",
pages = "1484",
doi = "10.3390/nu12051484"
}

Stojković, L. S., Jovanović, I. G., Živković, M., Zec, M., Đurić, T., Životić, I., Kuveljić, J., Kolaković, A., Kolić, I., Đorđević, A., Glibetić, M., Alavantić, D.,& Stanković, A.. (2020). The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk. in Nutrients, 12(5), 1484.
https://doi.org/10.3390/nu12051484

Stojković LS, Jovanović IG, Živković M, Zec M, Đurić T, Životić I, Kuveljić J, Kolaković A, Kolić I, Đorđević A, Glibetić M, Alavantić D, Stanković A. The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk. in Nutrients. 2020;12(5):1484.
doi:10.3390/nu12051484 .

Stojković, Ljiljana S., Jovanović, Ivan G., Živković, Maja, Zec, Manja, Đurić, Tamara, Životić, Ivan, Kuveljić, Jovana, Kolaković, Ana, Kolić, Ivana, Đorđević, Ana, Glibetić, Marija, Alavantić, Dragan, Stanković, Aleksandra, "The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk" in Nutrients, 12, no. 5 (2020):1484,
https://doi.org/10.3390/nu12051484 . .

TY  - CONF
AU  - Kolaković, Ana
AU  - Živković, Maja
AU  - Đurić, Tamara
AU  - Končar, Igor
AU  - Stanković, Aleksandra
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S002191501830707X
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7952
C3  - Atherosclerosis
T1  - The expression of renin-angiotensin-system components (ACE, ACE2 and collectrin (TMEM27)) in the human carotid plaques depending on gender and plaque phenotype
VL  - 275
SP  - e135
DO  - 10.1016/j.atherosclerosis.2018.06.395
ER  - 

@conference{
author = "Kolaković, Ana and Živković, Maja and Đurić, Tamara and Končar, Igor and Stanković, Aleksandra",
year = "2018",
journal = "Atherosclerosis",
title = "The expression of renin-angiotensin-system components (ACE, ACE2 and collectrin (TMEM27)) in the human carotid plaques depending on gender and plaque phenotype",
volume = "275",
pages = "e135",
doi = "10.1016/j.atherosclerosis.2018.06.395"
}

Kolaković, A., Živković, M., Đurić, T., Končar, I.,& Stanković, A.. (2018). The expression of renin-angiotensin-system components (ACE, ACE2 and collectrin (TMEM27)) in the human carotid plaques depending on gender and plaque phenotype. in Atherosclerosis, 275, e135.
https://doi.org/10.1016/j.atherosclerosis.2018.06.395

Kolaković A, Živković M, Đurić T, Končar I, Stanković A. The expression of renin-angiotensin-system components (ACE, ACE2 and collectrin (TMEM27)) in the human carotid plaques depending on gender and plaque phenotype. in Atherosclerosis. 2018;275:e135.
doi:10.1016/j.atherosclerosis.2018.06.395 .

Kolaković, Ana, Živković, Maja, Đurić, Tamara, Končar, Igor, Stanković, Aleksandra, "The expression of renin-angiotensin-system components (ACE, ACE2 and collectrin (TMEM27)) in the human carotid plaques depending on gender and plaque phenotype" in Atherosclerosis, 275 (2018):e135,
https://doi.org/10.1016/j.atherosclerosis.2018.06.395 . .

TY  - JOUR
AU  - Živković, Maja
AU  - Kolaković, Ana
AU  - Stojković, Ljiljana S.
AU  - Dinčić, Evica
AU  - Kostic, Smiljana
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1016
AB  - The components of renin-angiotensin system, such as angiotensin-converting enzyme (ACE), angiotensin II and angiotensin II receptor type 1 and 2 (AT1R and AT2R), are expressed in the central nervous system and leukocytes and proposed to be involved in the inflammation and pathogenesis of multiple sclerosis (MS). ACE I/D, AT1R 1166A/C and AT2R-1332A/G are functional polymorphisms associated with phenotypes of diverse chronic inflammatory diseases. The aim of this study was to investigate the association between ACE I/D, AT1R 1166A/C and AT2R-1332A/G gene polymorphisms and MS in Serbian population. A total of 470 MS patients and 478 controls participated in the study. Allele-specific polymerase chain reaction (PCR) was performed for genotyping of the ACE polymorphism. The AT1R and AT2R genotyping was done by duplex PCR and restriction fragment length polymorphism analysis. Both ACE homozygotes, II and DD, were significantly overrepresented in MS patients, compared to controls (chi(2) test p = 0.03). Neither genotype nor allele frequencies of AT1R 1166A/C polymorphism were significantly different between patients and controls. Significant overrepresentation of AT2R-1332 AA genotype in female patients, compared to female controls, was detected (OR = 1.67, 95%CI = 1.13-2.49, chi(2) test p = 0.01), suggesting that this genotype could be a gender-specific genetic risk factor for MS. (C) 2016 Elsevier B.V. All rights reserved.
T2  - Journal of the Neurological Sciences
T1  - Renin-angiotensin system gene polymorphisms as risk factors for multiple sclerosis
VL  - 363
SP  - 29
EP  - 32
DO  - 10.1016/j.jns.2016.02.026
ER  - 

@article{
author = "Živković, Maja and Kolaković, Ana and Stojković, Ljiljana S. and Dinčić, Evica and Kostic, Smiljana and Alavantić, Dragan and Stanković, Aleksandra",
year = "2016",
abstract = "The components of renin-angiotensin system, such as angiotensin-converting enzyme (ACE), angiotensin II and angiotensin II receptor type 1 and 2 (AT1R and AT2R), are expressed in the central nervous system and leukocytes and proposed to be involved in the inflammation and pathogenesis of multiple sclerosis (MS). ACE I/D, AT1R 1166A/C and AT2R-1332A/G are functional polymorphisms associated with phenotypes of diverse chronic inflammatory diseases. The aim of this study was to investigate the association between ACE I/D, AT1R 1166A/C and AT2R-1332A/G gene polymorphisms and MS in Serbian population. A total of 470 MS patients and 478 controls participated in the study. Allele-specific polymerase chain reaction (PCR) was performed for genotyping of the ACE polymorphism. The AT1R and AT2R genotyping was done by duplex PCR and restriction fragment length polymorphism analysis. Both ACE homozygotes, II and DD, were significantly overrepresented in MS patients, compared to controls (chi(2) test p = 0.03). Neither genotype nor allele frequencies of AT1R 1166A/C polymorphism were significantly different between patients and controls. Significant overrepresentation of AT2R-1332 AA genotype in female patients, compared to female controls, was detected (OR = 1.67, 95%CI = 1.13-2.49, chi(2) test p = 0.01), suggesting that this genotype could be a gender-specific genetic risk factor for MS. (C) 2016 Elsevier B.V. All rights reserved.",
journal = "Journal of the Neurological Sciences",
title = "Renin-angiotensin system gene polymorphisms as risk factors for multiple sclerosis",
volume = "363",
pages = "29-32",
doi = "10.1016/j.jns.2016.02.026"
}

Živković, M., Kolaković, A., Stojković, L. S., Dinčić, E., Kostic, S., Alavantić, D.,& Stanković, A.. (2016). Renin-angiotensin system gene polymorphisms as risk factors for multiple sclerosis. in Journal of the Neurological Sciences, 363, 29-32.
https://doi.org/10.1016/j.jns.2016.02.026

Živković M, Kolaković A, Stojković LS, Dinčić E, Kostic S, Alavantić D, Stanković A. Renin-angiotensin system gene polymorphisms as risk factors for multiple sclerosis. in Journal of the Neurological Sciences. 2016;363:29-32.
doi:10.1016/j.jns.2016.02.026 .

Živković, Maja, Kolaković, Ana, Stojković, Ljiljana S., Dinčić, Evica, Kostic, Smiljana, Alavantić, Dragan, Stanković, Aleksandra, "Renin-angiotensin system gene polymorphisms as risk factors for multiple sclerosis" in Journal of the Neurological Sciences, 363 (2016):29-32,
https://doi.org/10.1016/j.jns.2016.02.026 . .

TY  - JOUR
AU  - Petrović, Nina
AU  - Kolaković, Ana
AU  - Stanković, Aleksandra
AU  - Lukić, Silvana
AU  - Sami, Ahmad
AU  - Živković, Maja
AU  - Mandušić, Vesna
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1023
AB  - BACKGROUND: Breast carcinoma is heterogeneous disease. Understanding the process of invasion and metastasis and the selection of the therapy for patients with breast carcinomas still remains difficult. MicroRNAs are powerful gene expression regulators. Because of inconsistent findings, we have analyzed potential difference in miR-155 levels in three breast cancer groups. OBJECTIVES: Our goals were to examine miR-155 expression levels in normal tissue, non-invasive and invasive breast carcinomas, and their association with standard clinical and pathological parameters and oncomiR-21, and to investigate the ability of miR-155 to separate invasive breast carcinomas with non-invasive component from pure invasive. METHODS: In the group of 40 breast tissue samples, relative expression levels of miR-155 were examined with stem-loop quantitative real-time PCR using TaqMan technology. RESULTS: The significant difference among four examined groups of the breast tissue was detected (p = 0.001). In the group of pure invasive tumors, patients with positive nodal status had significantly higher miR-155 levels (p = 0.046). CONCLUSION: Our results suggest that miR-155 might be involved in breast cancer pathogenesis and in tumor spreading to the lymph nodes, and that it might be used as biomarker for additional stratification of patients with invasive breast carcinomas with non-invasive component.
T2  - Cancer Biomarkers
T1  - miR-155 expression level changes might be associated with initial phases of breast cancer pathogenesis and lymph-node metastasis
VL  - 16
IS  - 3
SP  - 385
EP  - 394
DO  - 10.3233/CBM-160577
ER  - 

@article{
author = "Petrović, Nina and Kolaković, Ana and Stanković, Aleksandra and Lukić, Silvana and Sami, Ahmad and Živković, Maja and Mandušić, Vesna",
year = "2016",
abstract = "BACKGROUND: Breast carcinoma is heterogeneous disease. Understanding the process of invasion and metastasis and the selection of the therapy for patients with breast carcinomas still remains difficult. MicroRNAs are powerful gene expression regulators. Because of inconsistent findings, we have analyzed potential difference in miR-155 levels in three breast cancer groups. OBJECTIVES: Our goals were to examine miR-155 expression levels in normal tissue, non-invasive and invasive breast carcinomas, and their association with standard clinical and pathological parameters and oncomiR-21, and to investigate the ability of miR-155 to separate invasive breast carcinomas with non-invasive component from pure invasive. METHODS: In the group of 40 breast tissue samples, relative expression levels of miR-155 were examined with stem-loop quantitative real-time PCR using TaqMan technology. RESULTS: The significant difference among four examined groups of the breast tissue was detected (p = 0.001). In the group of pure invasive tumors, patients with positive nodal status had significantly higher miR-155 levels (p = 0.046). CONCLUSION: Our results suggest that miR-155 might be involved in breast cancer pathogenesis and in tumor spreading to the lymph nodes, and that it might be used as biomarker for additional stratification of patients with invasive breast carcinomas with non-invasive component.",
journal = "Cancer Biomarkers",
title = "miR-155 expression level changes might be associated with initial phases of breast cancer pathogenesis and lymph-node metastasis",
volume = "16",
number = "3",
pages = "385-394",
doi = "10.3233/CBM-160577"
}

Petrović, N., Kolaković, A., Stanković, A., Lukić, S., Sami, A., Živković, M.,& Mandušić, V.. (2016). miR-155 expression level changes might be associated with initial phases of breast cancer pathogenesis and lymph-node metastasis. in Cancer Biomarkers, 16(3), 385-394.
https://doi.org/10.3233/CBM-160577

Petrović N, Kolaković A, Stanković A, Lukić S, Sami A, Živković M, Mandušić V. miR-155 expression level changes might be associated with initial phases of breast cancer pathogenesis and lymph-node metastasis. in Cancer Biomarkers. 2016;16(3):385-394.
doi:10.3233/CBM-160577 .

Petrović, Nina, Kolaković, Ana, Stanković, Aleksandra, Lukić, Silvana, Sami, Ahmad, Živković, Maja, Mandušić, Vesna, "miR-155 expression level changes might be associated with initial phases of breast cancer pathogenesis and lymph-node metastasis" in Cancer Biomarkers, 16, no. 3 (2016):385-394,
https://doi.org/10.3233/CBM-160577 . .

TY  - JOUR
AU  - Stanković, Aleksandra
AU  - Kolaković, Ana
AU  - Živković, Maja
AU  - Đurić, Tamara
AU  - Bundalo, Maja M.
AU  - Končar, Igor
AU  - Davidović, Lazar
AU  - Alavantić, Dragan
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1045
AB  - Background and Aims: The principal biologic effects of the renin-angiotensin system are mediated by activation of the AT1R receptor. The microRNA miR-155 regulates AT1R expression, with both its, and AT1Rs activity, linked to atherosclerosis. Target sites for miR-155 lie within the 3 UTR of the human AT1R gene, and include the AT1R A1166C polymorphism. Thus far, only levels of circulating miR-155 have been investigated with respect to A1166C genotypes. We hypothesized that the A1166C polymorphism could correlate with different, ultra-sonographically defined plaque phenotypes, as well as with an altered expression of AT1R mRNA and protein in human carotid plaques (CP), and altered expression of miR-155 in patients with advanced atherosclerosis. Methods: Our study cohort comprised 411 patients with advanced carotid atherosclerosis (298 hyperechoic; 113 hypoechoic plaques). PCR analyses identified A1166C genotypes; quantitative real-time PCR determined AT1R and miR-155 expression levels, with AT1R protein expression evaluated by western blot. Results: Genotypes containing the C allele bore a significant association with the hypoechoic plaque phenotype (adjusted OR 1.87, 95% CI 1.16-3.00, p = 0.01). The expression of AT1R mRNA and miR-155 were significantly up-regulated in the CPs of CC genotype carriers compared to the AA/AC genotypes (p = 0.032, p = 0.015, respectively). AT1R protein expression was also significantly higher for CC genotypes (p LT 0.01). Conclusion: Our results indicate that the AT1R A1166C polymorphism impacts an ultrasonographicallydefined human plaque phenotype, with intra-plaque AT1R and miR-155 expression altered in advanced carotid atherosclerosis. Validation and replication of these data should contribute to an improved personalized therapy with which to prevent carotid atherosclerosis. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
PB  - Elsevier
T2  - Atherosclerosis
T1  - Angiotensin receptor type 1 polymorphism A1166C is associated with altered AT1R and miR-155 expression in carotid plaque tissue and development of hypoechoic carotid plaques
VL  - 248
SP  - 132
EP  - 139
DO  - 10.1016/j.atherosclerosis.2016.02.032
ER  - 

@article{
author = "Stanković, Aleksandra and Kolaković, Ana and Živković, Maja and Đurić, Tamara and Bundalo, Maja M. and Končar, Igor and Davidović, Lazar and Alavantić, Dragan",
year = "2016",
abstract = "Background and Aims: The principal biologic effects of the renin-angiotensin system are mediated by activation of the AT1R receptor. The microRNA miR-155 regulates AT1R expression, with both its, and AT1Rs activity, linked to atherosclerosis. Target sites for miR-155 lie within the 3 UTR of the human AT1R gene, and include the AT1R A1166C polymorphism. Thus far, only levels of circulating miR-155 have been investigated with respect to A1166C genotypes. We hypothesized that the A1166C polymorphism could correlate with different, ultra-sonographically defined plaque phenotypes, as well as with an altered expression of AT1R mRNA and protein in human carotid plaques (CP), and altered expression of miR-155 in patients with advanced atherosclerosis. Methods: Our study cohort comprised 411 patients with advanced carotid atherosclerosis (298 hyperechoic; 113 hypoechoic plaques). PCR analyses identified A1166C genotypes; quantitative real-time PCR determined AT1R and miR-155 expression levels, with AT1R protein expression evaluated by western blot. Results: Genotypes containing the C allele bore a significant association with the hypoechoic plaque phenotype (adjusted OR 1.87, 95% CI 1.16-3.00, p = 0.01). The expression of AT1R mRNA and miR-155 were significantly up-regulated in the CPs of CC genotype carriers compared to the AA/AC genotypes (p = 0.032, p = 0.015, respectively). AT1R protein expression was also significantly higher for CC genotypes (p LT 0.01). Conclusion: Our results indicate that the AT1R A1166C polymorphism impacts an ultrasonographicallydefined human plaque phenotype, with intra-plaque AT1R and miR-155 expression altered in advanced carotid atherosclerosis. Validation and replication of these data should contribute to an improved personalized therapy with which to prevent carotid atherosclerosis. (C) 2016 Elsevier Ireland Ltd. All rights reserved.",
publisher = "Elsevier",
journal = "Atherosclerosis",
title = "Angiotensin receptor type 1 polymorphism A1166C is associated with altered AT1R and miR-155 expression in carotid plaque tissue and development of hypoechoic carotid plaques",
volume = "248",
pages = "132-139",
doi = "10.1016/j.atherosclerosis.2016.02.032"
}

Stanković, A., Kolaković, A., Živković, M., Đurić, T., Bundalo, M. M., Končar, I., Davidović, L.,& Alavantić, D.. (2016). Angiotensin receptor type 1 polymorphism A1166C is associated with altered AT1R and miR-155 expression in carotid plaque tissue and development of hypoechoic carotid plaques. in Atherosclerosis
Elsevier., 248, 132-139.
https://doi.org/10.1016/j.atherosclerosis.2016.02.032

Stanković A, Kolaković A, Živković M, Đurić T, Bundalo MM, Končar I, Davidović L, Alavantić D. Angiotensin receptor type 1 polymorphism A1166C is associated with altered AT1R and miR-155 expression in carotid plaque tissue and development of hypoechoic carotid plaques. in Atherosclerosis. 2016;248:132-139.
doi:10.1016/j.atherosclerosis.2016.02.032 .

Stanković, Aleksandra, Kolaković, Ana, Živković, Maja, Đurić, Tamara, Bundalo, Maja M., Končar, Igor, Davidović, Lazar, Alavantić, Dragan, "Angiotensin receptor type 1 polymorphism A1166C is associated with altered AT1R and miR-155 expression in carotid plaque tissue and development of hypoechoic carotid plaques" in Atherosclerosis, 248 (2016):132-139,
https://doi.org/10.1016/j.atherosclerosis.2016.02.032 . .

TY  - JOUR
AU  - Kolaković, Ana
AU  - Stanković, Aleksandra
AU  - Đurić, Tamara
AU  - Živković, Maja
AU  - Končar, Igor
AU  - Davidović, Lazar
AU  - Radak, Đorđe J.
AU  - Alavantić, Dragan
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1123
AB  - Background: The angiotensin II type 2 receptor (AT2R) - 1332 A/G polymorphism has been denoted as functional and associated with certain cardiovascular disease phenotypes. However, there are no studies considering the association of this gene polymorphism with carotid atherosclerosis (CA) and cerebrovascular events. Therefore, the aim of our study was to investigate a possible association of the AT2R - 1332 A/G polymorphism with the occurrence of carotid plaques (CPs) and history of cerebrovascular insult (CVI) in advanced CA. Methods: The study group included 381 controls and 509 patients with CA consecutively admitted for endarterectomy. Genotyping was determined by polymerase chain reaction-restriction fragment length polymorphism method. The association was analyzed separately for males and females because the AT2R gene is located on the X chromosome. Results: The AT2R - 1332 GG genotype was associated with the advanced CA in the female study group (recessive model of inheritance, AA+AG versus GG; adjusted odds ratio [OR] = 2.25; 95% confidence interval [CI] 1.17-4.33; P=.01). In the male subgroup of patients with CA, the significant overrepresentation of G/- hemizygote was detected in patients with CVI compared to male patients without this event (crude OR = 2.05, 95% CI 1.20-3.50, P=.008). Conclusions: This study suggests a gender-specific association between the AT2R -1332 A/G polymorphism and the occurrence of CP and the history of CVI in advanced CA, but further replication studies are needed. (C) 2016 National Stroke Association. Published by Elsevier Inc. All rights reserved.
T2  - Journal of Stroke and Cerebrovascular Diseases
T1  - Gender-Specific Association between Angiotensin II Type 2 Receptor-1332 A/G Gene Polymorphism and Advanced Carotid Atherosclerosis
VL  - 25
IS  - 7
SP  - 1622
EP  - 1630
DO  - 10.1016/j.jstrokecerebrovasdis.2016.03.011
ER  - 

@article{
author = "Kolaković, Ana and Stanković, Aleksandra and Đurić, Tamara and Živković, Maja and Končar, Igor and Davidović, Lazar and Radak, Đorđe J. and Alavantić, Dragan",
year = "2016",
abstract = "Background: The angiotensin II type 2 receptor (AT2R) - 1332 A/G polymorphism has been denoted as functional and associated with certain cardiovascular disease phenotypes. However, there are no studies considering the association of this gene polymorphism with carotid atherosclerosis (CA) and cerebrovascular events. Therefore, the aim of our study was to investigate a possible association of the AT2R - 1332 A/G polymorphism with the occurrence of carotid plaques (CPs) and history of cerebrovascular insult (CVI) in advanced CA. Methods: The study group included 381 controls and 509 patients with CA consecutively admitted for endarterectomy. Genotyping was determined by polymerase chain reaction-restriction fragment length polymorphism method. The association was analyzed separately for males and females because the AT2R gene is located on the X chromosome. Results: The AT2R - 1332 GG genotype was associated with the advanced CA in the female study group (recessive model of inheritance, AA+AG versus GG; adjusted odds ratio [OR] = 2.25; 95% confidence interval [CI] 1.17-4.33; P=.01). In the male subgroup of patients with CA, the significant overrepresentation of G/- hemizygote was detected in patients with CVI compared to male patients without this event (crude OR = 2.05, 95% CI 1.20-3.50, P=.008). Conclusions: This study suggests a gender-specific association between the AT2R -1332 A/G polymorphism and the occurrence of CP and the history of CVI in advanced CA, but further replication studies are needed. (C) 2016 National Stroke Association. Published by Elsevier Inc. All rights reserved.",
journal = "Journal of Stroke and Cerebrovascular Diseases",
title = "Gender-Specific Association between Angiotensin II Type 2 Receptor-1332 A/G Gene Polymorphism and Advanced Carotid Atherosclerosis",
volume = "25",
number = "7",
pages = "1622-1630",
doi = "10.1016/j.jstrokecerebrovasdis.2016.03.011"
}

Kolaković, A., Stanković, A., Đurić, T., Živković, M., Končar, I., Davidović, L., Radak, Đ. J.,& Alavantić, D.. (2016). Gender-Specific Association between Angiotensin II Type 2 Receptor-1332 A/G Gene Polymorphism and Advanced Carotid Atherosclerosis. in Journal of Stroke and Cerebrovascular Diseases, 25(7), 1622-1630.
https://doi.org/10.1016/j.jstrokecerebrovasdis.2016.03.011

Kolaković A, Stanković A, Đurić T, Živković M, Končar I, Davidović L, Radak ĐJ, Alavantić D. Gender-Specific Association between Angiotensin II Type 2 Receptor-1332 A/G Gene Polymorphism and Advanced Carotid Atherosclerosis. in Journal of Stroke and Cerebrovascular Diseases. 2016;25(7):1622-1630.
doi:10.1016/j.jstrokecerebrovasdis.2016.03.011 .

Kolaković, Ana, Stanković, Aleksandra, Đurić, Tamara, Živković, Maja, Končar, Igor, Davidović, Lazar, Radak, Đorđe J., Alavantić, Dragan, "Gender-Specific Association between Angiotensin II Type 2 Receptor-1332 A/G Gene Polymorphism and Advanced Carotid Atherosclerosis" in Journal of Stroke and Cerebrovascular Diseases, 25, no. 7 (2016):1622-1630,
https://doi.org/10.1016/j.jstrokecerebrovasdis.2016.03.011 . .

TY  - CONF
AU  - Živković, Maja
AU  - Kolaković, Ana
AU  - Đurić, Tamara
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7075
C3  - Atherosclerosis
T1  - The At1 Receptor Polymorphism Rs5186 and Mir-155 Expression in Human Carotid Plaques
VL  - 241
IS  - 1
SP  - E85
EP  - E85
DO  - 10.1016/j.atherosclerosis.2015.04.298
ER  - 

@conference{
author = "Živković, Maja and Kolaković, Ana and Đurić, Tamara and Alavantić, Dragan and Stanković, Aleksandra",
year = "2015",
journal = "Atherosclerosis",
title = "The At1 Receptor Polymorphism Rs5186 and Mir-155 Expression in Human Carotid Plaques",
volume = "241",
number = "1",
pages = "E85-E85",
doi = "10.1016/j.atherosclerosis.2015.04.298"
}

Živković, M., Kolaković, A., Đurić, T., Alavantić, D.,& Stanković, A.. (2015). The At1 Receptor Polymorphism Rs5186 and Mir-155 Expression in Human Carotid Plaques. in Atherosclerosis, 241(1), E85-E85.
https://doi.org/10.1016/j.atherosclerosis.2015.04.298

Živković M, Kolaković A, Đurić T, Alavantić D, Stanković A. The At1 Receptor Polymorphism Rs5186 and Mir-155 Expression in Human Carotid Plaques. in Atherosclerosis. 2015;241(1):E85-E85.
doi:10.1016/j.atherosclerosis.2015.04.298 .

Živković, Maja, Kolaković, Ana, Đurić, Tamara, Alavantić, Dragan, Stanković, Aleksandra, "The At1 Receptor Polymorphism Rs5186 and Mir-155 Expression in Human Carotid Plaques" in Atherosclerosis, 241, no. 1 (2015):E85-E85,
https://doi.org/10.1016/j.atherosclerosis.2015.04.298 . .

TY  - JOUR
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Đurić, Tamara
AU  - Končar, Igor
AU  - Kolaković, Ana
AU  - Đurđević, Vladimir
AU  - Davidović, Lazar
AU  - Alavantić, Dragan
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5869
AB  - Glutathione S-transferases (GSTs) carry out a wide range of functions in cells, such as detoxification of endogenous compounds, removal of reactive oxygen species, and even catalysis of reactions in metabolic pathways beyond detoxification. Based on previous research, GSTM1 and GSTT1 might modify the risk of atherosclerosis. The aim of our study was to analyze the possible association of GSTM1 and GSTT1 gene polymorphisms with the occurrence of carotid plaque (CP); and biochemical parameters of oxidative stress, lipid profile and inflammation, in 346 consecutive patients with advanced atherosclerosis that underwent endarterectomy. A multiplex polymerase chain reaction (PCR) method was used to detect the deletions in GSTM1 and GSTT1 genes in the genomic DNA in 346 patients and 330 controls. The adjusted OR for CP presence (adjusted for age, gender, smoking, hypertension, BMI, HDLC, TG) was 0.24, 95 %CI 0.08-0.7, p LT 0.01 for GSTT1 null and 1.13, 95 %CI 0.62-2.07, p = 0.7 for GSTM1 null genotype. We found significantly lower plasma lipoprotein (a) (Lp(a)) levels in GSTT1 null compared to wild-type genotype carriers in patient group (20.68 +/- A 26.02 mg/dl vs. 40.66 +/- A 42.89 mg/dl, mean +/- A SD, p = 0.04). The serum interleukin-6 (IL-6) values were significantly influenced by both GST polymorphisms in patients with CP. Our results, showing the significant reduction of GSTT1 deletions in patients with CP, suggest involvement of GSTs in carotid atherosclerosis. This study shows additional view of the possible role of GSTs in advanced chronic inflammatory disease of vascular system, but the confirmation in a larger studies in different populations are needed.
T2  - Molecular Biology Reports
T1  - Effects of glutathione S-transferase T1 and M1 deletions on advanced carotid atherosclerosis, oxidative, lipid and inflammatory parameters
VL  - 41
IS  - 2
SP  - 1157
EP  - 1164
DO  - 10.1007/s11033-013-2962-z
ER  - 

@article{
author = "Živković, Maja and Stanković, Aleksandra and Đurić, Tamara and Končar, Igor and Kolaković, Ana and Đurđević, Vladimir and Davidović, Lazar and Alavantić, Dragan",
year = "2014",
abstract = "Glutathione S-transferases (GSTs) carry out a wide range of functions in cells, such as detoxification of endogenous compounds, removal of reactive oxygen species, and even catalysis of reactions in metabolic pathways beyond detoxification. Based on previous research, GSTM1 and GSTT1 might modify the risk of atherosclerosis. The aim of our study was to analyze the possible association of GSTM1 and GSTT1 gene polymorphisms with the occurrence of carotid plaque (CP); and biochemical parameters of oxidative stress, lipid profile and inflammation, in 346 consecutive patients with advanced atherosclerosis that underwent endarterectomy. A multiplex polymerase chain reaction (PCR) method was used to detect the deletions in GSTM1 and GSTT1 genes in the genomic DNA in 346 patients and 330 controls. The adjusted OR for CP presence (adjusted for age, gender, smoking, hypertension, BMI, HDLC, TG) was 0.24, 95 %CI 0.08-0.7, p LT 0.01 for GSTT1 null and 1.13, 95 %CI 0.62-2.07, p = 0.7 for GSTM1 null genotype. We found significantly lower plasma lipoprotein (a) (Lp(a)) levels in GSTT1 null compared to wild-type genotype carriers in patient group (20.68 +/- A 26.02 mg/dl vs. 40.66 +/- A 42.89 mg/dl, mean +/- A SD, p = 0.04). The serum interleukin-6 (IL-6) values were significantly influenced by both GST polymorphisms in patients with CP. Our results, showing the significant reduction of GSTT1 deletions in patients with CP, suggest involvement of GSTs in carotid atherosclerosis. This study shows additional view of the possible role of GSTs in advanced chronic inflammatory disease of vascular system, but the confirmation in a larger studies in different populations are needed.",
journal = "Molecular Biology Reports",
title = "Effects of glutathione S-transferase T1 and M1 deletions on advanced carotid atherosclerosis, oxidative, lipid and inflammatory parameters",
volume = "41",
number = "2",
pages = "1157-1164",
doi = "10.1007/s11033-013-2962-z"
}

Živković, M., Stanković, A., Đurić, T., Končar, I., Kolaković, A., Đurđević, V., Davidović, L.,& Alavantić, D.. (2014). Effects of glutathione S-transferase T1 and M1 deletions on advanced carotid atherosclerosis, oxidative, lipid and inflammatory parameters. in Molecular Biology Reports, 41(2), 1157-1164.
https://doi.org/10.1007/s11033-013-2962-z

Živković M, Stanković A, Đurić T, Končar I, Kolaković A, Đurđević V, Davidović L, Alavantić D. Effects of glutathione S-transferase T1 and M1 deletions on advanced carotid atherosclerosis, oxidative, lipid and inflammatory parameters. in Molecular Biology Reports. 2014;41(2):1157-1164.
doi:10.1007/s11033-013-2962-z .

Živković, Maja, Stanković, Aleksandra, Đurić, Tamara, Končar, Igor, Kolaković, Ana, Đurđević, Vladimir, Davidović, Lazar, Alavantić, Dragan, "Effects of glutathione S-transferase T1 and M1 deletions on advanced carotid atherosclerosis, oxidative, lipid and inflammatory parameters" in Molecular Biology Reports, 41, no. 2 (2014):1157-1164,
https://doi.org/10.1007/s11033-013-2962-z . .

TY  - JOUR
AU  - Ješić, Snežana
AU  - Jotić, Ana
AU  - Tomanović, Nada
AU  - Živković, Maja
AU  - Kolaković, Ana
AU  - Stanković, Aleksandra
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6051
AB  - Objectives: The objectives were to detect and compare the expression of toll-like receptors (TLRs) 2, 4 and nuclear factor kappa B in mucosal lesions of chronic otitis. Methods: Fifty-five tissue samples obtained from children and adults operated on for otitis were investigated by semiquantitative immunohistochemical methods using polyclonal antibodies for TLR 2, 4 and NFkB. Kruskal-Wallis, Mann-Whitney, and Kendalls tau rank correlation tests were used. Results: Stronger expression of TLR2, 4 was found in inflamed mucosa than in the control for children and adults (TLR2: H = 23.86, P LT .001; TLR4: H = 22.80, P LT .001) (TLR2: H = 17.53, P LT .001; TLR4: H = 11.99, P LT .001); in cholesteatoma perimatrix compared to tubotympanic lesions in children (TLR2: H = 11.06, P = .004; TLR4: H = 10.61, P = .005) and adults (TLR2: H = 10.73, P =.013; TLR4: H = 9.65, P = .021). No differences were found in NFkB expression (H = 0.042, P = .99). Significant correlations were found for all pairs of molecules in cholesteatoma and tubotympanic mucosa of adults (TLR2, 4: P = .002, P LT .001; TLR2-NfkB: P = .032, P = .021; TLR4-NFkB: P =.035, P = .0013), only TLR4-NFkB in tubotympanic otitis of children (P = .026). Conclusions: Toll-like receptors 2, 4 and NFkB mediate inflammation in cholesteatoma and mucosal lesions of tubotympanic otitis in children and adults. Significant correlations between all pairs of molecules in all samples were detected in adults, but only TLR4-NFkB in children.
T2  - Annals of Otology Rhinology and Laryngology
T1  - Expression of Toll-Like Receptors 2, 4 and Nuclear Factor Kappa B in Mucosal Lesions of Human Otitis: Pattern and Relationship in a Clinical Immunohistochemical Study
VL  - 123
IS  - 6
SP  - 434
EP  - 441
DO  - 10.1177/0003489414527229
ER  - 

@article{
author = "Ješić, Snežana and Jotić, Ana and Tomanović, Nada and Živković, Maja and Kolaković, Ana and Stanković, Aleksandra",
year = "2014",
abstract = "Objectives: The objectives were to detect and compare the expression of toll-like receptors (TLRs) 2, 4 and nuclear factor kappa B in mucosal lesions of chronic otitis. Methods: Fifty-five tissue samples obtained from children and adults operated on for otitis were investigated by semiquantitative immunohistochemical methods using polyclonal antibodies for TLR 2, 4 and NFkB. Kruskal-Wallis, Mann-Whitney, and Kendalls tau rank correlation tests were used. Results: Stronger expression of TLR2, 4 was found in inflamed mucosa than in the control for children and adults (TLR2: H = 23.86, P LT .001; TLR4: H = 22.80, P LT .001) (TLR2: H = 17.53, P LT .001; TLR4: H = 11.99, P LT .001); in cholesteatoma perimatrix compared to tubotympanic lesions in children (TLR2: H = 11.06, P = .004; TLR4: H = 10.61, P = .005) and adults (TLR2: H = 10.73, P =.013; TLR4: H = 9.65, P = .021). No differences were found in NFkB expression (H = 0.042, P = .99). Significant correlations were found for all pairs of molecules in cholesteatoma and tubotympanic mucosa of adults (TLR2, 4: P = .002, P LT .001; TLR2-NfkB: P = .032, P = .021; TLR4-NFkB: P =.035, P = .0013), only TLR4-NFkB in tubotympanic otitis of children (P = .026). Conclusions: Toll-like receptors 2, 4 and NFkB mediate inflammation in cholesteatoma and mucosal lesions of tubotympanic otitis in children and adults. Significant correlations between all pairs of molecules in all samples were detected in adults, but only TLR4-NFkB in children.",
journal = "Annals of Otology Rhinology and Laryngology",
title = "Expression of Toll-Like Receptors 2, 4 and Nuclear Factor Kappa B in Mucosal Lesions of Human Otitis: Pattern and Relationship in a Clinical Immunohistochemical Study",
volume = "123",
number = "6",
pages = "434-441",
doi = "10.1177/0003489414527229"
}

Ješić, S., Jotić, A., Tomanović, N., Živković, M., Kolaković, A.,& Stanković, A.. (2014). Expression of Toll-Like Receptors 2, 4 and Nuclear Factor Kappa B in Mucosal Lesions of Human Otitis: Pattern and Relationship in a Clinical Immunohistochemical Study. in Annals of Otology Rhinology and Laryngology, 123(6), 434-441.
https://doi.org/10.1177/0003489414527229

Ješić S, Jotić A, Tomanović N, Živković M, Kolaković A, Stanković A. Expression of Toll-Like Receptors 2, 4 and Nuclear Factor Kappa B in Mucosal Lesions of Human Otitis: Pattern and Relationship in a Clinical Immunohistochemical Study. in Annals of Otology Rhinology and Laryngology. 2014;123(6):434-441.
doi:10.1177/0003489414527229 .

Ješić, Snežana, Jotić, Ana, Tomanović, Nada, Živković, Maja, Kolaković, Ana, Stanković, Aleksandra, "Expression of Toll-Like Receptors 2, 4 and Nuclear Factor Kappa B in Mucosal Lesions of Human Otitis: Pattern and Relationship in a Clinical Immunohistochemical Study" in Annals of Otology Rhinology and Laryngology, 123, no. 6 (2014):434-441,
https://doi.org/10.1177/0003489414527229 . .

TY  - CONF
AU  - Jotić, Ana
AU  - Kuveljić, Jovana
AU  - Kolaković, Ana
AU  - Kolić, Ivana
AU  - Živković, Maja
AU  - Jesić, Snežana
AU  - Stanković, Aleksandra
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12387
AB  - Toll like receptors (TLRs) are the pattern recognition receptors, important for the innate immune system, the first line of defense against bacterial infections which are the main cause of otitis media (OM). Our aim was to investigate the association of TLR4 (Thr399Ile and Asp299Gly) and TLR2 (Arg753Gln) most common polymorphisms with OM prevalence as well as with morphologic changes of middle ear during chronic otitis media. We had 111 controls and 186 patients divided into three groups of COM: nonsuppurative OM, suppurative OM and cholesteatoma. Frequencies of the genotypes of the TLR 2 gene polymorphism were significantly different between men and women having nonsuppurative OM (p=0.03).
PB  - Belgrade : Serbian Genetic Society
C3  - V Congress of the Serbian Genetic Society : Book of abstracts
T1  - Toll like receptors 2 and 4 polymorphisms in chronic otitis media
SP  - 52
EP  - 52
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12387
ER  - 

@conference{
author = "Jotić, Ana and Kuveljić, Jovana and Kolaković, Ana and Kolić, Ivana and Živković, Maja and Jesić, Snežana and Stanković, Aleksandra",
year = "2014",
abstract = "Toll like receptors (TLRs) are the pattern recognition receptors, important for the innate immune system, the first line of defense against bacterial infections which are the main cause of otitis media (OM). Our aim was to investigate the association of TLR4 (Thr399Ile and Asp299Gly) and TLR2 (Arg753Gln) most common polymorphisms with OM prevalence as well as with morphologic changes of middle ear during chronic otitis media. We had 111 controls and 186 patients divided into three groups of COM: nonsuppurative OM, suppurative OM and cholesteatoma. Frequencies of the genotypes of the TLR 2 gene polymorphism were significantly different between men and women having nonsuppurative OM (p=0.03).",
publisher = "Belgrade : Serbian Genetic Society",
journal = "V Congress of the Serbian Genetic Society : Book of abstracts",
title = "Toll like receptors 2 and 4 polymorphisms in chronic otitis media",
pages = "52-52",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12387"
}

Jotić, A., Kuveljić, J., Kolaković, A., Kolić, I., Živković, M., Jesić, S.,& Stanković, A.. (2014). Toll like receptors 2 and 4 polymorphisms in chronic otitis media. in V Congress of the Serbian Genetic Society : Book of abstracts
Belgrade : Serbian Genetic Society., 52-52.
https://hdl.handle.net/21.15107/rcub_vinar_12387

Jotić A, Kuveljić J, Kolaković A, Kolić I, Živković M, Jesić S, Stanković A. Toll like receptors 2 and 4 polymorphisms in chronic otitis media. in V Congress of the Serbian Genetic Society : Book of abstracts. 2014;:52-52.
https://hdl.handle.net/21.15107/rcub_vinar_12387 .

Jotić, Ana, Kuveljić, Jovana, Kolaković, Ana, Kolić, Ivana, Živković, Maja, Jesić, Snežana, Stanković, Aleksandra, "Toll like receptors 2 and 4 polymorphisms in chronic otitis media" in V Congress of the Serbian Genetic Society : Book of abstracts (2014):52-52,
https://hdl.handle.net/21.15107/rcub_vinar_12387 .

TY  - THES
AU  - Kolaković, Ana P.
PY  - 2013-12-27
UR  - https://nardus.mpn.gov.rs/handle/123456789/2146
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=1119
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:7874/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024653746
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12701
AB  - Karotidna ateroskleroza (KA) je hronična bolest koja započinje aktivacijom i nakupljanjem inflamatornih ćelija koje se pune lipidima u zidu arterijskog krvnog suda. Aterosklerotski plak sadrži lipidno jezgro, fibroznu kapu, akumulira glatke mišićne ćelije (GMĆ) i proteine ekstraćelijskog matriksa (EĆM).Renin-angiotensin system (RAS) ima ključnu ulogu u aktivaciji inflamacije u zidu krvnog suda. Glavni molekuli RAS su: angiotenzin-konvertujući enzim (ACE), angiotenzin-konvertujući enzim-homolog (ACE2), angiotenzin II receptori tipa 1 (ATR1) i tipa 2 (ATR2), ACE2 homolog (TMEM 27) i regulatorna RNK (miR-155). Angiotenzin II (Ang II) je glavni efektorni molekul RAS koji se sintetiše aktivnošću ACE, a razgrađuje aktivnošću ACE2. Ang II ostvaruje svoje efekte vezujuću se za ATR1 i ATR2. Efekti Ang II imaju važnu ulogu u regulaciji vaskularne homeostaze i to su: vazokonstrikcija, migracija, proliferacija i hipertrofija GMĆ, povećana sinteza EĆM i povećana produkcija matriks-metaloproteinaza (MMPs). Ekspresija ATR1 regulisana je sa miR-155. Balans između aktivacije i represije RAS može biti odlučujući u patološkom remodelovanju zida krvnog suda i patogenezi KA. Zato je važno ispitati funkcionalnu aktivaciju RAS sistema na lokalnom (tkivnom) nivou u karotidama i to na nivou ekspresije gena koji kodiraju za komponente RAS, enzime i receptore. Ekspresija gena je regulisana preko prisustva/odsustva različitih alelnih varijanti funkcionalnih polimorfizama u genima za RAS.Genetičko-epidemiološka studija gena RAS je urađena po pacijenti - kontrole dizajnu, na tri gena i tri polimorfizma. Ova studija obuhvatila je 750 ispitanika oba pola iz populacije Srbije, 505 pacijenata i 246 kontrola iste etničke pripadnosti. Urađena je studija asocijacije polimorfizma I/D u genu za ACE, polimorfizma A1166C u genu za ATR1 i polimorfizma A/G -1332 u genu za ATR2 sa nastankom KA, kliničkim parametrima KA, fenotipom plaka (stabilni (SAP) i nestabilni (NAP)), kliničkim događajima (prolazni ishemijski događaj (TIA) i cerebrovaskularni insult (CVI)) u Srbiji. Isti polimorfizmi su asocirani i sa ekspresijom gena u tkivu plaku KA. Analiza ekspresije gena RAS na nivou iRNK je urađena po istom dizajnu, na šest gena...
AB  - The carotid atherosclerosis (CA) is chronic disease, which begins with activation inflammatory cells and accumulation lipid in these cells within blood vessel wall. Atherosclerotic plaque containing a lipid core, fibrous cap, accumulates smooth muscle cells (GMC) and proteins of the extracellular matrix (ECM).The renin-angiotensin system (RAS) has a key role in activation of inflammation within blood vessel wall. The main components of the system are: angiotensin I-converting enzyme (ACE), angiotensin converting enzyme homolog (ACE2), angiotensin II receptor type 1 (ATR1), angiotensin II receptor type 2 (ATR2), ACE2 homolog (TMEM27) and regulatory RNA (miR-155). Angiotensin II (Ang II) is the main effector molecule of the RAS, which is synthesized by the activity of ACE and is cleaved by ACE2. Ang II achieves its effects by binding to ATR1 and ATR2. These effects has an important role in the regulation of vascular homeostasis and include the following: vasoconstriction, vascular smooth muscle cells (SMC) migration, proliferation and hypertrophy, increased synthesis of extracellular matrix (ECM) and enhanced production of matrix metalloproteinases (MMPs). The expression of ATR1 is regulated by the miR-155. The balance between activation and repression of RAS can be decisive in pathological remodeling of the vessel wall and the pathogenesis CA. It is therefore important to examine the functional activation of RAS at the local (tissue) level in carotid vessel wall at level of expression genes encoding for components of the RAS, enzymes and receptors. The gene expression of components of RAS is regulated through the presence/absence of different allelic variants of functional polymorphisms in these genes.The genetic epidemiological study for RAS genes was performed by case-control design and consisted of three genes and three polymorphisms within these genes. This study included 750 subjects of both sexes from the Serbian population, 505 patients and 246 controls of the same ethnic background. It is done genetic association study the ACE I/D, ATR1 A1166C and ATR2 A/G -1332 polymorphisms with occurrence of CA, clinical parameters of CA, (phenotypes of plaques (stable (SP) and unstable (USP)), clinical events of disease (transient ischemic attack (TIA) and cerebrovascular insult (CVI)) in Serbia...
PB  - Универзитет у Београду, Биолошки факултет
T2  - Универзитет у Београду
T1  - Genetičko-epidemiološka analiza i analiza ekspresije gena renin-angiotenzin sistema (RAS) u karotidnoj aterosklerozi kod čoveka
T1  - Genetic-epidemiological analysis and analysis of gene expression of the renin-angiotensin system (RAS) in carotid atherosclerosis in humans
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12701
ER  - 

@phdthesis{
author = "Kolaković, Ana P.",
year = "2013-12-27",
abstract = "Karotidna ateroskleroza (KA) je hronična bolest koja započinje aktivacijom i nakupljanjem inflamatornih ćelija koje se pune lipidima u zidu arterijskog krvnog suda. Aterosklerotski plak sadrži lipidno jezgro, fibroznu kapu, akumulira glatke mišićne ćelije (GMĆ) i proteine ekstraćelijskog matriksa (EĆM).Renin-angiotensin system (RAS) ima ključnu ulogu u aktivaciji inflamacije u zidu krvnog suda. Glavni molekuli RAS su: angiotenzin-konvertujući enzim (ACE), angiotenzin-konvertujući enzim-homolog (ACE2), angiotenzin II receptori tipa 1 (ATR1) i tipa 2 (ATR2), ACE2 homolog (TMEM 27) i regulatorna RNK (miR-155). Angiotenzin II (Ang II) je glavni efektorni molekul RAS koji se sintetiše aktivnošću ACE, a razgrađuje aktivnošću ACE2. Ang II ostvaruje svoje efekte vezujuću se za ATR1 i ATR2. Efekti Ang II imaju važnu ulogu u regulaciji vaskularne homeostaze i to su: vazokonstrikcija, migracija, proliferacija i hipertrofija GMĆ, povećana sinteza EĆM i povećana produkcija matriks-metaloproteinaza (MMPs). Ekspresija ATR1 regulisana je sa miR-155. Balans između aktivacije i represije RAS može biti odlučujući u patološkom remodelovanju zida krvnog suda i patogenezi KA. Zato je važno ispitati funkcionalnu aktivaciju RAS sistema na lokalnom (tkivnom) nivou u karotidama i to na nivou ekspresije gena koji kodiraju za komponente RAS, enzime i receptore. Ekspresija gena je regulisana preko prisustva/odsustva različitih alelnih varijanti funkcionalnih polimorfizama u genima za RAS.Genetičko-epidemiološka studija gena RAS je urađena po pacijenti - kontrole dizajnu, na tri gena i tri polimorfizma. Ova studija obuhvatila je 750 ispitanika oba pola iz populacije Srbije, 505 pacijenata i 246 kontrola iste etničke pripadnosti. Urađena je studija asocijacije polimorfizma I/D u genu za ACE, polimorfizma A1166C u genu za ATR1 i polimorfizma A/G -1332 u genu za ATR2 sa nastankom KA, kliničkim parametrima KA, fenotipom plaka (stabilni (SAP) i nestabilni (NAP)), kliničkim događajima (prolazni ishemijski događaj (TIA) i cerebrovaskularni insult (CVI)) u Srbiji. Isti polimorfizmi su asocirani i sa ekspresijom gena u tkivu plaku KA. Analiza ekspresije gena RAS na nivou iRNK je urađena po istom dizajnu, na šest gena..., The carotid atherosclerosis (CA) is chronic disease, which begins with activation inflammatory cells and accumulation lipid in these cells within blood vessel wall. Atherosclerotic plaque containing a lipid core, fibrous cap, accumulates smooth muscle cells (GMC) and proteins of the extracellular matrix (ECM).The renin-angiotensin system (RAS) has a key role in activation of inflammation within blood vessel wall. The main components of the system are: angiotensin I-converting enzyme (ACE), angiotensin converting enzyme homolog (ACE2), angiotensin II receptor type 1 (ATR1), angiotensin II receptor type 2 (ATR2), ACE2 homolog (TMEM27) and regulatory RNA (miR-155). Angiotensin II (Ang II) is the main effector molecule of the RAS, which is synthesized by the activity of ACE and is cleaved by ACE2. Ang II achieves its effects by binding to ATR1 and ATR2. These effects has an important role in the regulation of vascular homeostasis and include the following: vasoconstriction, vascular smooth muscle cells (SMC) migration, proliferation and hypertrophy, increased synthesis of extracellular matrix (ECM) and enhanced production of matrix metalloproteinases (MMPs). The expression of ATR1 is regulated by the miR-155. The balance between activation and repression of RAS can be decisive in pathological remodeling of the vessel wall and the pathogenesis CA. It is therefore important to examine the functional activation of RAS at the local (tissue) level in carotid vessel wall at level of expression genes encoding for components of the RAS, enzymes and receptors. The gene expression of components of RAS is regulated through the presence/absence of different allelic variants of functional polymorphisms in these genes.The genetic epidemiological study for RAS genes was performed by case-control design and consisted of three genes and three polymorphisms within these genes. This study included 750 subjects of both sexes from the Serbian population, 505 patients and 246 controls of the same ethnic background. It is done genetic association study the ACE I/D, ATR1 A1166C and ATR2 A/G -1332 polymorphisms with occurrence of CA, clinical parameters of CA, (phenotypes of plaques (stable (SP) and unstable (USP)), clinical events of disease (transient ischemic attack (TIA) and cerebrovascular insult (CVI)) in Serbia...",
publisher = "Универзитет у Београду, Биолошки факултет",
journal = "Универзитет у Београду",
title = "Genetičko-epidemiološka analiza i analiza ekspresije gena renin-angiotenzin sistema (RAS) u karotidnoj aterosklerozi kod čoveka, Genetic-epidemiological analysis and analysis of gene expression of the renin-angiotensin system (RAS) in carotid atherosclerosis in humans",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12701"
}

Kolaković, A. P.. (2013-12-27). Genetičko-epidemiološka analiza i analiza ekspresije gena renin-angiotenzin sistema (RAS) u karotidnoj aterosklerozi kod čoveka. in Универзитет у Београду
Универзитет у Београду, Биолошки факултет..
https://hdl.handle.net/21.15107/rcub_vinar_12701

Kolaković AP. Genetičko-epidemiološka analiza i analiza ekspresije gena renin-angiotenzin sistema (RAS) u karotidnoj aterosklerozi kod čoveka. in Универзитет у Београду. 2013;.
https://hdl.handle.net/21.15107/rcub_vinar_12701 .

Kolaković, Ana P., "Genetičko-epidemiološka analiza i analiza ekspresije gena renin-angiotenzin sistema (RAS) u karotidnoj aterosklerozi kod čoveka" in Универзитет у Београду (2013-12-27),
https://hdl.handle.net/21.15107/rcub_vinar_12701 .

TY  - CONF
AU  - Kolaković, Ana
AU  - Živković, Maja
AU  - Končar, Igor
AU  - Jovanović, Ivan G.
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5821
C3  - Cerebrovascular Diseases
T1  - The angiotensin II type 2 receptor-1332 A/G gene polymorphism (rs1403543) is associated with stroke in males patients with carotid atherosclerosis
VL  - 35
SP  - 536
EP  - 536
UR  - https://hdl.handle.net/21.15107/rcub_vinar_5821
ER  - 

@conference{
author = "Kolaković, Ana and Živković, Maja and Končar, Igor and Jovanović, Ivan G. and Đurić, Tamara and Stanković, Aleksandra",
year = "2013",
journal = "Cerebrovascular Diseases",
title = "The angiotensin II type 2 receptor-1332 A/G gene polymorphism (rs1403543) is associated with stroke in males patients with carotid atherosclerosis",
volume = "35",
pages = "536-536",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5821"
}

Kolaković, A., Živković, M., Končar, I., Jovanović, I. G., Đurić, T.,& Stanković, A.. (2013). The angiotensin II type 2 receptor-1332 A/G gene polymorphism (rs1403543) is associated with stroke in males patients with carotid atherosclerosis. in Cerebrovascular Diseases, 35, 536-536.
https://hdl.handle.net/21.15107/rcub_vinar_5821

Kolaković A, Živković M, Končar I, Jovanović IG, Đurić T, Stanković A. The angiotensin II type 2 receptor-1332 A/G gene polymorphism (rs1403543) is associated with stroke in males patients with carotid atherosclerosis. in Cerebrovascular Diseases. 2013;35:536-536.
https://hdl.handle.net/21.15107/rcub_vinar_5821 .

Kolaković, Ana, Živković, Maja, Končar, Igor, Jovanović, Ivan G., Đurić, Tamara, Stanković, Aleksandra, "The angiotensin II type 2 receptor-1332 A/G gene polymorphism (rs1403543) is associated with stroke in males patients with carotid atherosclerosis" in Cerebrovascular Diseases, 35 (2013):536-536,
https://hdl.handle.net/21.15107/rcub_vinar_5821 .

TY  - JOUR
AU  - Kolaković, Ana
AU  - Živković, Maja
AU  - Radak, Đorđe J.
AU  - Đurić, Tamara
AU  - Končar, Igor
AU  - Davidović, Lazar
AU  - Dinčić, Dragan
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4719
AB  - Introduction: The ACE I/D polymorphism was mostly investigated in association with intima-media thickness, rarely with severe atherosclerotic phenotype. Materials and methods: We investigated the association of I/D polymorphism with severe carotid atherosclerosis (CA) (stenosis GT 70%) in asymptomatic and symptomatic patients undergoing carotid endarterectomy. The 504 patients subjected to endarterectomy and 492 healthy controls from a population in Serbia were investigated as a case-control study. Results: The univariate logistic regression analysis revealed ACE DD as a significant risk factor for severe CA (odds ratio [OR] = 1.3, 95% confidence interval [CI] 1.0-1.7, p = 0.04). After adjustment for the common risk factors (age, hypertension, smoking, and HDL) ACE was no longer significant. However, we found a significant independent influence of DD genotype on plaque presence in a normotensive subgroup of patients (OR 1.8, CI 1.2-3.0, p = 0.01, corrected for multiple testing). In symptomatic patients D allele carriers were significantly more frequent compared with asymptomatic patients (OR 1.6 CI 1.0-2.6, p = 0.05). Conclusions: Our data suggests that ACE I/D is not an independent risk factor for severe CA. On the other hand, a significant independent genetic influence of ACE I/D appeared in normotensive and symptomatic patients with severe CA. This should be considered in further research toward resolving the complex genetic background of severe CA phenotype.
T2  - Journal of the Renin-Angiotensin-Aldosterone System
T1  - The association of ACE I/D gene polymorphism with severe carotid atherosclerosis in patients undergoing carotid endarterectomy
VL  - 13
IS  - 1
SP  - 141
EP  - 147
DO  - 10.1177/1470320311423271
ER  - 

@article{
author = "Kolaković, Ana and Živković, Maja and Radak, Đorđe J. and Đurić, Tamara and Končar, Igor and Davidović, Lazar and Dinčić, Dragan and Alavantić, Dragan and Stanković, Aleksandra",
year = "2012",
abstract = "Introduction: The ACE I/D polymorphism was mostly investigated in association with intima-media thickness, rarely with severe atherosclerotic phenotype. Materials and methods: We investigated the association of I/D polymorphism with severe carotid atherosclerosis (CA) (stenosis GT 70%) in asymptomatic and symptomatic patients undergoing carotid endarterectomy. The 504 patients subjected to endarterectomy and 492 healthy controls from a population in Serbia were investigated as a case-control study. Results: The univariate logistic regression analysis revealed ACE DD as a significant risk factor for severe CA (odds ratio [OR] = 1.3, 95% confidence interval [CI] 1.0-1.7, p = 0.04). After adjustment for the common risk factors (age, hypertension, smoking, and HDL) ACE was no longer significant. However, we found a significant independent influence of DD genotype on plaque presence in a normotensive subgroup of patients (OR 1.8, CI 1.2-3.0, p = 0.01, corrected for multiple testing). In symptomatic patients D allele carriers were significantly more frequent compared with asymptomatic patients (OR 1.6 CI 1.0-2.6, p = 0.05). Conclusions: Our data suggests that ACE I/D is not an independent risk factor for severe CA. On the other hand, a significant independent genetic influence of ACE I/D appeared in normotensive and symptomatic patients with severe CA. This should be considered in further research toward resolving the complex genetic background of severe CA phenotype.",
journal = "Journal of the Renin-Angiotensin-Aldosterone System",
title = "The association of ACE I/D gene polymorphism with severe carotid atherosclerosis in patients undergoing carotid endarterectomy",
volume = "13",
number = "1",
pages = "141-147",
doi = "10.1177/1470320311423271"
}

Kolaković, A., Živković, M., Radak, Đ. J., Đurić, T., Končar, I., Davidović, L., Dinčić, D., Alavantić, D.,& Stanković, A.. (2012). The association of ACE I/D gene polymorphism with severe carotid atherosclerosis in patients undergoing carotid endarterectomy. in Journal of the Renin-Angiotensin-Aldosterone System, 13(1), 141-147.
https://doi.org/10.1177/1470320311423271

Kolaković A, Živković M, Radak ĐJ, Đurić T, Končar I, Davidović L, Dinčić D, Alavantić D, Stanković A. The association of ACE I/D gene polymorphism with severe carotid atherosclerosis in patients undergoing carotid endarterectomy. in Journal of the Renin-Angiotensin-Aldosterone System. 2012;13(1):141-147.
doi:10.1177/1470320311423271 .

Kolaković, Ana, Živković, Maja, Radak, Đorđe J., Đurić, Tamara, Končar, Igor, Davidović, Lazar, Dinčić, Dragan, Alavantić, Dragan, Stanković, Aleksandra, "The association of ACE I/D gene polymorphism with severe carotid atherosclerosis in patients undergoing carotid endarterectomy" in Journal of the Renin-Angiotensin-Aldosterone System, 13, no. 1 (2012):141-147,
https://doi.org/10.1177/1470320311423271 . .

TY  - JOUR
AU  - Živković, Maja
AU  - Kolaković, Ana
AU  - Radak, Đorđe J.
AU  - Dinčić, Dragan
AU  - Radak, Sandra
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4823
AB  - Atherosclerosis is known as an inflammatory disease in which a recruitment of leukocytes to the endothelium wall represents a preliminary step of the initiation and the development of disease. The P-selectin glycoprotein ligand (PSGL-1) seems to be the major molecule mediating leukocyte-endothelium interactions and leukocyte rolling on stimulated endothelium. There are limited number of studies reporting on association of Met62Ile SNP in PSGL-1 gene and the risk for atherosclerosis. The aim of this study was to analyze possible association of this polymorphism with an advanced carotid atherosclerosis and biochemical markers of inflammation and haemostasis. The 275 patients consecutively admitted for carotid endarterectomy with stenosis GT 70% and 256 controls of the same ethnic origin were included in the study. The Met62Ile genotypes were determined by PCR RFLP. The Ile/Ile homozygotes had significantly higher CRP compared to the other genotypes in patients. Female patients had Ile allele dose-dependent association with the carotid plaque presence (Met/Met vs. Met/Ile vs. Ile/Ile; OR 1, OR 2.02, CI 1.0-4.08, OR 4.08, CI 1.0-16.81, respectively, p = 0.04). Our results suggest the impact of PSGL-1 Met62Ile polymorphism on inflammation in advanced atherosclerosis. We observed the sex-differential association of Met62Ile with advanced carotid atherosclerosis. Studies in larger and different populations should validate and further examine the suggested role of genetic variations in PSGL-1 with atherosclerosis and thrombosis.
T2  - Molecular Biology Reports
T1  - The sex-specific association of Met62Ile gene polymorphism in P-selectin glycoprotein ligand (PSGL-1) with carotid plaque presence: preliminary study
VL  - 39
IS  - 6
SP  - 6479
EP  - 6485
DO  - 10.1007/s11033-012-1475-5
ER  - 

@article{
author = "Živković, Maja and Kolaković, Ana and Radak, Đorđe J. and Dinčić, Dragan and Radak, Sandra and Đurić, Tamara and Stanković, Aleksandra",
year = "2012",
abstract = "Atherosclerosis is known as an inflammatory disease in which a recruitment of leukocytes to the endothelium wall represents a preliminary step of the initiation and the development of disease. The P-selectin glycoprotein ligand (PSGL-1) seems to be the major molecule mediating leukocyte-endothelium interactions and leukocyte rolling on stimulated endothelium. There are limited number of studies reporting on association of Met62Ile SNP in PSGL-1 gene and the risk for atherosclerosis. The aim of this study was to analyze possible association of this polymorphism with an advanced carotid atherosclerosis and biochemical markers of inflammation and haemostasis. The 275 patients consecutively admitted for carotid endarterectomy with stenosis GT 70% and 256 controls of the same ethnic origin were included in the study. The Met62Ile genotypes were determined by PCR RFLP. The Ile/Ile homozygotes had significantly higher CRP compared to the other genotypes in patients. Female patients had Ile allele dose-dependent association with the carotid plaque presence (Met/Met vs. Met/Ile vs. Ile/Ile; OR 1, OR 2.02, CI 1.0-4.08, OR 4.08, CI 1.0-16.81, respectively, p = 0.04). Our results suggest the impact of PSGL-1 Met62Ile polymorphism on inflammation in advanced atherosclerosis. We observed the sex-differential association of Met62Ile with advanced carotid atherosclerosis. Studies in larger and different populations should validate and further examine the suggested role of genetic variations in PSGL-1 with atherosclerosis and thrombosis.",
journal = "Molecular Biology Reports",
title = "The sex-specific association of Met62Ile gene polymorphism in P-selectin glycoprotein ligand (PSGL-1) with carotid plaque presence: preliminary study",
volume = "39",
number = "6",
pages = "6479-6485",
doi = "10.1007/s11033-012-1475-5"
}

Živković, M., Kolaković, A., Radak, Đ. J., Dinčić, D., Radak, S., Đurić, T.,& Stanković, A.. (2012). The sex-specific association of Met62Ile gene polymorphism in P-selectin glycoprotein ligand (PSGL-1) with carotid plaque presence: preliminary study. in Molecular Biology Reports, 39(6), 6479-6485.
https://doi.org/10.1007/s11033-012-1475-5

Živković M, Kolaković A, Radak ĐJ, Dinčić D, Radak S, Đurić T, Stanković A. The sex-specific association of Met62Ile gene polymorphism in P-selectin glycoprotein ligand (PSGL-1) with carotid plaque presence: preliminary study. in Molecular Biology Reports. 2012;39(6):6479-6485.
doi:10.1007/s11033-012-1475-5 .

Živković, Maja, Kolaković, Ana, Radak, Đorđe J., Dinčić, Dragan, Radak, Sandra, Đurić, Tamara, Stanković, Aleksandra, "The sex-specific association of Met62Ile gene polymorphism in P-selectin glycoprotein ligand (PSGL-1) with carotid plaque presence: preliminary study" in Molecular Biology Reports, 39, no. 6 (2012):6479-6485,
https://doi.org/10.1007/s11033-012-1475-5 . .

TY  - CONF
AU  - Milovanović, Branislav
AU  - Bojić, Tijana
AU  - Kolaković, Ana
AU  - Alavantić, Dragan
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12698
AB  - INTRODUCTION: Polymorphisms of renin-angiotensin system (RAS) in hypertensive patients may be associated with
ECG parameters (QT, QRS complex, QRS axis, pNN50, rms SD). QT interval prolongation on 12-lead ECG is a predictor
of sudden cardiac death due to ventricular arrhythmias (1). Hypertension is the state where RAS activity is modulated,
and this can remodel the cardiac ion channels, resulting in prolongation (QT interval) and redirection of ventricular
depolarization (QRS axis) and repolarization. Cardiac ion channels can be affected by such factors as the strech of the
myocites by high blood pressure (2). To our knowledge no investigation has been done on the association of RAS
genetic polymorphisms with the ECG parameters QT interval, QRS axis and time domain indexes of heart rate variability
pNN50 and rmsSD. AIM. The aim of the study was to investigate the association of RAS genetic polymorphisms (ACE
I/D, ATR1 1166 A/C, ATR2 1332 A/G) with ECG parameters (QT, QRS axis, pNN50 and rmsSD).
METHODS. The study was conducted on 36 Serbian hypertensive untreated patients (age (mean±SD)=51.6±9.9
years, 17 female and 19 male) who were ECG monitored (Schiller multiparameter monitor) and taken whole blood
sample for genetic analysis. DNA was extracted on the basis of phenol-chlorophorm method while genotypisation was
done by PCR-restriction fragment lenght polymorphism method (3,4). Statistical analysis was performed using
statistical package of SPSS program (SPSS 17.0).
RESULTS. The results are summarized in Table 1. CONCLUSIONS: Our data are in line with the data in the literature that ACE D allele carriers intensify left ventricular
hypertrophy, myocardial fibrosis and hypertension (5, 6). These changes contribute to electrophysiologic remodeling of
myocardium resulting in change of parameters like QT interval and QRS axis. Parameters of short term heart rate
variability showed tendency to be associated to RAS polymorphisms. Further investigations are needed to bring us
definite conclusions.
PB  - Belgrade : Serbian neurocardiological society
C3  - NEUROCARD 2012 : 4th International Symposium on Neurocardiology : 3rd International Symposium on Noninvasive Electrocardiology : Program and the book of abstracts
T1  - The associations of genetic polymorphisms of reninangiotensin system with ECG - parameters of short-term analysis (Schiller multiparameter monitor): the preliminary study
SP  - 142
EP  - 142
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12698
ER  - 

@conference{
author = "Milovanović, Branislav and Bojić, Tijana and Kolaković, Ana and Alavantić, Dragan",
year = "2012",
abstract = "INTRODUCTION: Polymorphisms of renin-angiotensin system (RAS) in hypertensive patients may be associated with
ECG parameters (QT, QRS complex, QRS axis, pNN50, rms SD). QT interval prolongation on 12-lead ECG is a predictor
of sudden cardiac death due to ventricular arrhythmias (1). Hypertension is the state where RAS activity is modulated,
and this can remodel the cardiac ion channels, resulting in prolongation (QT interval) and redirection of ventricular
depolarization (QRS axis) and repolarization. Cardiac ion channels can be affected by such factors as the strech of the
myocites by high blood pressure (2). To our knowledge no investigation has been done on the association of RAS
genetic polymorphisms with the ECG parameters QT interval, QRS axis and time domain indexes of heart rate variability
pNN50 and rmsSD. AIM. The aim of the study was to investigate the association of RAS genetic polymorphisms (ACE
I/D, ATR1 1166 A/C, ATR2 1332 A/G) with ECG parameters (QT, QRS axis, pNN50 and rmsSD).
METHODS. The study was conducted on 36 Serbian hypertensive untreated patients (age (mean±SD)=51.6±9.9
years, 17 female and 19 male) who were ECG monitored (Schiller multiparameter monitor) and taken whole blood
sample for genetic analysis. DNA was extracted on the basis of phenol-chlorophorm method while genotypisation was
done by PCR-restriction fragment lenght polymorphism method (3,4). Statistical analysis was performed using
statistical package of SPSS program (SPSS 17.0).
RESULTS. The results are summarized in Table 1. CONCLUSIONS: Our data are in line with the data in the literature that ACE D allele carriers intensify left ventricular
hypertrophy, myocardial fibrosis and hypertension (5, 6). These changes contribute to electrophysiologic remodeling of
myocardium resulting in change of parameters like QT interval and QRS axis. Parameters of short term heart rate
variability showed tendency to be associated to RAS polymorphisms. Further investigations are needed to bring us
definite conclusions.",
publisher = "Belgrade : Serbian neurocardiological society",
journal = "NEUROCARD 2012 : 4th International Symposium on Neurocardiology : 3rd International Symposium on Noninvasive Electrocardiology : Program and the book of abstracts",
title = "The associations of genetic polymorphisms of reninangiotensin system with ECG - parameters of short-term analysis (Schiller multiparameter monitor): the preliminary study",
pages = "142-142",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12698"
}

Milovanović, B., Bojić, T., Kolaković, A.,& Alavantić, D.. (2012). The associations of genetic polymorphisms of reninangiotensin system with ECG - parameters of short-term analysis (Schiller multiparameter monitor): the preliminary study. in NEUROCARD 2012 : 4th International Symposium on Neurocardiology : 3rd International Symposium on Noninvasive Electrocardiology : Program and the book of abstracts
Belgrade : Serbian neurocardiological society., 142-142.
https://hdl.handle.net/21.15107/rcub_vinar_12698

Milovanović B, Bojić T, Kolaković A, Alavantić D. The associations of genetic polymorphisms of reninangiotensin system with ECG - parameters of short-term analysis (Schiller multiparameter monitor): the preliminary study. in NEUROCARD 2012 : 4th International Symposium on Neurocardiology : 3rd International Symposium on Noninvasive Electrocardiology : Program and the book of abstracts. 2012;:142-142.
https://hdl.handle.net/21.15107/rcub_vinar_12698 .

Milovanović, Branislav, Bojić, Tijana, Kolaković, Ana, Alavantić, Dragan, "The associations of genetic polymorphisms of reninangiotensin system with ECG - parameters of short-term analysis (Schiller multiparameter monitor): the preliminary study" in NEUROCARD 2012 : 4th International Symposium on Neurocardiology : 3rd International Symposium on Noninvasive Electrocardiology : Program and the book of abstracts (2012):142-142,
https://hdl.handle.net/21.15107/rcub_vinar_12698 .

TY  - CONF
AU  - Kolaković, Ana
AU  - Živković, Maja
AU  - Radak, Đorđe J.
AU  - Končar, Igor
AU  - Đurić, Tamara
AU  - Davidović, L.
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6939
C3  - Atherosclerosis Supplements
T1  - Association of Angiotensin Ii Type 1 Receptor+1166 A/C Gene Polymorphism with Human Carotid Plaque Vulnerability
VL  - 12
IS  - 1
SP  - 111
EP  - 111
DO  - 10.1016/S1567-5688(11)70526-8
ER  - 

@conference{
author = "Kolaković, Ana and Živković, Maja and Radak, Đorđe J. and Končar, Igor and Đurić, Tamara and Davidović, L. and Alavantić, Dragan and Stanković, Aleksandra",
year = "2011",
journal = "Atherosclerosis Supplements",
title = "Association of Angiotensin Ii Type 1 Receptor+1166 A/C Gene Polymorphism with Human Carotid Plaque Vulnerability",
volume = "12",
number = "1",
pages = "111-111",
doi = "10.1016/S1567-5688(11)70526-8"
}

Kolaković, A., Živković, M., Radak, Đ. J., Končar, I., Đurić, T., Davidović, L., Alavantić, D.,& Stanković, A.. (2011). Association of Angiotensin Ii Type 1 Receptor+1166 A/C Gene Polymorphism with Human Carotid Plaque Vulnerability. in Atherosclerosis Supplements, 12(1), 111-111.
https://doi.org/10.1016/S1567-5688(11)70526-8

Kolaković A, Živković M, Radak ĐJ, Končar I, Đurić T, Davidović L, Alavantić D, Stanković A. Association of Angiotensin Ii Type 1 Receptor+1166 A/C Gene Polymorphism with Human Carotid Plaque Vulnerability. in Atherosclerosis Supplements. 2011;12(1):111-111.
doi:10.1016/S1567-5688(11)70526-8 .

Kolaković, Ana, Živković, Maja, Radak, Đorđe J., Končar, Igor, Đurić, Tamara, Davidović, L., Alavantić, Dragan, Stanković, Aleksandra, "Association of Angiotensin Ii Type 1 Receptor+1166 A/C Gene Polymorphism with Human Carotid Plaque Vulnerability" in Atherosclerosis Supplements, 12, no. 1 (2011):111-111,
https://doi.org/10.1016/S1567-5688(11)70526-8 . .

TY  - CONF
AU  - Kolaković, Ana
AU  - Končar, Igor
AU  - Stančić, O.
AU  - Davidović, L.
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
AU  - Živković, Maja
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6940
C3  - Atherosclerosis Supplements
T1  - The M62i Gene Polymorphism in P-Selectin Glycoprotein Ligand (Psgl-1) with Carotid Atherosclerosis: a Preliminar Study
VL  - 12
IS  - 1
SP  - 126
EP  - 126
DO  - 10.1016/S1567-5688(11)70600-6
ER  - 

@conference{
author = "Kolaković, Ana and Končar, Igor and Stančić, O. and Davidović, L. and Alavantić, Dragan and Stanković, Aleksandra and Živković, Maja",
year = "2011",
journal = "Atherosclerosis Supplements",
title = "The M62i Gene Polymorphism in P-Selectin Glycoprotein Ligand (Psgl-1) with Carotid Atherosclerosis: a Preliminar Study",
volume = "12",
number = "1",
pages = "126-126",
doi = "10.1016/S1567-5688(11)70600-6"
}

Kolaković, A., Končar, I., Stančić, O., Davidović, L., Alavantić, D., Stanković, A.,& Živković, M.. (2011). The M62i Gene Polymorphism in P-Selectin Glycoprotein Ligand (Psgl-1) with Carotid Atherosclerosis: a Preliminar Study. in Atherosclerosis Supplements, 12(1), 126-126.
https://doi.org/10.1016/S1567-5688(11)70600-6

Kolaković A, Končar I, Stančić O, Davidović L, Alavantić D, Stanković A, Živković M. The M62i Gene Polymorphism in P-Selectin Glycoprotein Ligand (Psgl-1) with Carotid Atherosclerosis: a Preliminar Study. in Atherosclerosis Supplements. 2011;12(1):126-126.
doi:10.1016/S1567-5688(11)70600-6 .

Kolaković, Ana, Končar, Igor, Stančić, O., Davidović, L., Alavantić, Dragan, Stanković, Aleksandra, Živković, Maja, "The M62i Gene Polymorphism in P-Selectin Glycoprotein Ligand (Psgl-1) with Carotid Atherosclerosis: a Preliminar Study" in Atherosclerosis Supplements, 12, no. 1 (2011):126-126,
https://doi.org/10.1016/S1567-5688(11)70600-6 . .

TY  - CONF
AU  - Živković, Maja
AU  - Popović, Milan
AU  - Stojković, Ljiljana S.
AU  - Radak, Đorđe J.
AU  - Končar, Igor
AU  - Davidovic, L.
AU  - Kolaković, Ana
AU  - Stanković, Aleksandra
AU  - Alavantić, Dragan
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6941
C3  - Atherosclerosis Supplements
T1  - The Association of Cxcl16 A181v Gene Polymorphism with Carotid Atherosclerosis in Patients Subjected to Endaterectomy
VL  - 12
IS  - 1
SP  - 153
EP  - 153
DO  - 10.1016/S1567-5688(11)70729-2
ER  - 

@conference{
author = "Živković, Maja and Popović, Milan and Stojković, Ljiljana S. and Radak, Đorđe J. and Končar, Igor and Davidovic, L. and Kolaković, Ana and Stanković, Aleksandra and Alavantić, Dragan",
year = "2011",
journal = "Atherosclerosis Supplements",
title = "The Association of Cxcl16 A181v Gene Polymorphism with Carotid Atherosclerosis in Patients Subjected to Endaterectomy",
volume = "12",
number = "1",
pages = "153-153",
doi = "10.1016/S1567-5688(11)70729-2"
}

Živković, M., Popović, M., Stojković, L. S., Radak, Đ. J., Končar, I., Davidovic, L., Kolaković, A., Stanković, A.,& Alavantić, D.. (2011). The Association of Cxcl16 A181v Gene Polymorphism with Carotid Atherosclerosis in Patients Subjected to Endaterectomy. in Atherosclerosis Supplements, 12(1), 153-153.
https://doi.org/10.1016/S1567-5688(11)70729-2

Živković M, Popović M, Stojković LS, Radak ĐJ, Končar I, Davidovic L, Kolaković A, Stanković A, Alavantić D. The Association of Cxcl16 A181v Gene Polymorphism with Carotid Atherosclerosis in Patients Subjected to Endaterectomy. in Atherosclerosis Supplements. 2011;12(1):153-153.
doi:10.1016/S1567-5688(11)70729-2 .

Živković, Maja, Popović, Milan, Stojković, Ljiljana S., Radak, Đorđe J., Končar, Igor, Davidovic, L., Kolaković, Ana, Stanković, Aleksandra, Alavantić, Dragan, "The Association of Cxcl16 A181v Gene Polymorphism with Carotid Atherosclerosis in Patients Subjected to Endaterectomy" in Atherosclerosis Supplements, 12, no. 1 (2011):153-153,
https://doi.org/10.1016/S1567-5688(11)70729-2 . .

TY  - JOUR
AU  - Đurić, Tamara
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Kolaković, Ana
AU  - Jekić, Đole
AU  - Selaković, Vesna
AU  - Alavantić, Dragan
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4129
AB  - Matrix metalloproteinases (MMPs) are involved in the remodeling of the extracellular matrix (ECM) in the arterial wall during atherogenesis. Collagens are the most abundant proteins in the ECM. MMP-8 is expressed by cells associated with the development of the atherosclerotic plaque. It cleaves collagen type I three times more potently than two other interstitial collagenases MMP-1 and MMP-13. The aim of this study was to investigate whether plasma MMP-8 values are associated with occurrence of carotid plaque (CP) and possible correlations with clinical and biochemical parameters in carotid atherosclerosis (CA) patients. Total plasma MMP-8 levels were quantified by ELISA in 63 patients with ultrasonographic evidence of CP presence and 12 controls. Plasma MMP-8 values were significantly higher in patients with CA compared with controls (median 23.36 ng/ml vs. 13.02 ng/ml, P LT 0.001) but they did not differ significantly according to gender, smoking and hypertensive status, associated diseases, and use of statins. Statistically significant positive correlations were observed between MMP-8 plasma values and C reactive protein (r=0.41, P=0.001), urea (r=0.50, P LT 0.001), aspartate transaminase (r=0.48, P=0.001), and creatinine levels (r=0.38, P=0.006). These results suggest association of MMP-8 plasma levels with occurrence of CP and correlation with certain biochemical markers. J. Clin. Lab. Anal. 24:246-251, 2010. (C) 2010 Wiley-Liss, Inc.
T2  - Journal of Clinical Laboratory Analysis
T1  - Plasma Levels of Matrix Metalloproteinase-8 in Patients With Carotid Atherosclerosis
VL  - 24
IS  - 4
SP  - 246
EP  - 251
DO  - 10.1002/jcla.20393
ER  - 

@article{
author = "Đurić, Tamara and Živković, Maja and Stanković, Aleksandra and Kolaković, Ana and Jekić, Đole and Selaković, Vesna and Alavantić, Dragan",
year = "2010",
abstract = "Matrix metalloproteinases (MMPs) are involved in the remodeling of the extracellular matrix (ECM) in the arterial wall during atherogenesis. Collagens are the most abundant proteins in the ECM. MMP-8 is expressed by cells associated with the development of the atherosclerotic plaque. It cleaves collagen type I three times more potently than two other interstitial collagenases MMP-1 and MMP-13. The aim of this study was to investigate whether plasma MMP-8 values are associated with occurrence of carotid plaque (CP) and possible correlations with clinical and biochemical parameters in carotid atherosclerosis (CA) patients. Total plasma MMP-8 levels were quantified by ELISA in 63 patients with ultrasonographic evidence of CP presence and 12 controls. Plasma MMP-8 values were significantly higher in patients with CA compared with controls (median 23.36 ng/ml vs. 13.02 ng/ml, P LT 0.001) but they did not differ significantly according to gender, smoking and hypertensive status, associated diseases, and use of statins. Statistically significant positive correlations were observed between MMP-8 plasma values and C reactive protein (r=0.41, P=0.001), urea (r=0.50, P LT 0.001), aspartate transaminase (r=0.48, P=0.001), and creatinine levels (r=0.38, P=0.006). These results suggest association of MMP-8 plasma levels with occurrence of CP and correlation with certain biochemical markers. J. Clin. Lab. Anal. 24:246-251, 2010. (C) 2010 Wiley-Liss, Inc.",
journal = "Journal of Clinical Laboratory Analysis",
title = "Plasma Levels of Matrix Metalloproteinase-8 in Patients With Carotid Atherosclerosis",
volume = "24",
number = "4",
pages = "246-251",
doi = "10.1002/jcla.20393"
}

Đurić, T., Živković, M., Stanković, A., Kolaković, A., Jekić, Đ., Selaković, V.,& Alavantić, D.. (2010). Plasma Levels of Matrix Metalloproteinase-8 in Patients With Carotid Atherosclerosis. in Journal of Clinical Laboratory Analysis, 24(4), 246-251.
https://doi.org/10.1002/jcla.20393

Đurić T, Živković M, Stanković A, Kolaković A, Jekić Đ, Selaković V, Alavantić D. Plasma Levels of Matrix Metalloproteinase-8 in Patients With Carotid Atherosclerosis. in Journal of Clinical Laboratory Analysis. 2010;24(4):246-251.
doi:10.1002/jcla.20393 .

Đurić, Tamara, Živković, Maja, Stanković, Aleksandra, Kolaković, Ana, Jekić, Đole, Selaković, Vesna, Alavantić, Dragan, "Plasma Levels of Matrix Metalloproteinase-8 in Patients With Carotid Atherosclerosis" in Journal of Clinical Laboratory Analysis, 24, no. 4 (2010):246-251,
https://doi.org/10.1002/jcla.20393 . .

TY  - CONF
AU  - Kolaković, Ana P.
AU  - Živković, Maja
AU  - Dinčić, Evica
AU  - Popović, Smiljana
AU  - Raičević, Ranko
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2010
PY  - 10 Supplement
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12791
AB  - Multiple  sclerosis  (MS)  is  a  complex  inflammatory,demyelinating disease of central nervous system (CNS). All the essen-tial components of the renin-angiotensin system (RAS) are presentedin  the  mammalian  brain.  The  angiotensin  II  (Ang  II),  biologicallyactive octapeptide is not only a vasoconstrictor, but also a pro-inflam-matory factor. Many of the classical and of the hypothetical functionsof brain Ang II are mediated by stimulation of AT1 receptors (AT1R).Brain AT2 receptors (AT2R) are highly expressed during development.In the adults, AT2R are restricted to areas predominantly involved inthe process of sensory information. The AT2R1332 A/G polymorph-ism was proposed to influence AT2R protein expression, and is themost studied polymorphism in this gene, in other diseases. Recently,the  striking  appearance  of  the  RAS  in  MS  brain  was  described.However, the role of AT2R remains to be clarified. Thus, the aim ofour  study  was  to  establish  if  there  is  an  association  between  AT2R1332 A/G gene polymorphism and predisposition of MS Methods:Subjected  group  consisted  of  122  female  and  70  malepatients with MS and 75 female and 50 male controls from populationof Serbia. Genotyping was done by PCR and restriction digestion withEcoRI enzyme.Results:The  genotype  and  allele  frequencies  for  AT2R1332A/Ggene  polymorphism  are  analyzed  separately  in  females  and  males,since this gene is located on X chromosome. We detected significantoverrepresentation of1332A/G AA genotype (OR 1.6, 95% CI:1.0–2.7, p<0.05) in female patients with MS compared to female controls.In hemizygous males we didn’t found any difference between patientsand controls.Conclusion:The  role  of  RAS  genes  in  MS  was  neglected  untilrecently.  Than,  it  was  shown  that  the  role  of  RAS  in  the  CNS  isbeyond  the  regulation  of  cardiovascular  function.  Until  now  AT2R(1332A/G)  gene  polymorphism  was widely studied  and associatedwith hypertension and other vascular disease. Until now, there wereno studies concerning role of Ang II receptor polymorphisms in MS.This study  suggest  possible  role  of  AT2R in  MS.  Further  studies areneeded to elucidate this result.
C3  - Multiple Sclerosis Journal
T1  - Angiotensin II receptor type 2 (AT2R) -1332 A/G gene polymorphism as a risk factor for multiple sclerosis
VL  - 16
SP  - S80
EP  - S81
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12791
ER  - 

@conference{
author = "Kolaković, Ana P. and Živković, Maja and Dinčić, Evica and Popović, Smiljana and Raičević, Ranko and Alavantić, Dragan and Stanković, Aleksandra",
year = "2010, 10 Supplement",
abstract = "Multiple  sclerosis  (MS)  is  a  complex  inflammatory,demyelinating disease of central nervous system (CNS). All the essen-tial components of the renin-angiotensin system (RAS) are presentedin  the  mammalian  brain.  The  angiotensin  II  (Ang  II),  biologicallyactive octapeptide is not only a vasoconstrictor, but also a pro-inflam-matory factor. Many of the classical and of the hypothetical functionsof brain Ang II are mediated by stimulation of AT1 receptors (AT1R).Brain AT2 receptors (AT2R) are highly expressed during development.In the adults, AT2R are restricted to areas predominantly involved inthe process of sensory information. The AT2R1332 A/G polymorph-ism was proposed to influence AT2R protein expression, and is themost studied polymorphism in this gene, in other diseases. Recently,the  striking  appearance  of  the  RAS  in  MS  brain  was  described.However, the role of AT2R remains to be clarified. Thus, the aim ofour  study  was  to  establish  if  there  is  an  association  between  AT2R1332 A/G gene polymorphism and predisposition of MS Methods:Subjected  group  consisted  of  122  female  and  70  malepatients with MS and 75 female and 50 male controls from populationof Serbia. Genotyping was done by PCR and restriction digestion withEcoRI enzyme.Results:The  genotype  and  allele  frequencies  for  AT2R1332A/Ggene  polymorphism  are  analyzed  separately  in  females  and  males,since this gene is located on X chromosome. We detected significantoverrepresentation of1332A/G AA genotype (OR 1.6, 95% CI:1.0–2.7, p<0.05) in female patients with MS compared to female controls.In hemizygous males we didn’t found any difference between patientsand controls.Conclusion:The  role  of  RAS  genes  in  MS  was  neglected  untilrecently.  Than,  it  was  shown  that  the  role  of  RAS  in  the  CNS  isbeyond  the  regulation  of  cardiovascular  function.  Until  now  AT2R(1332A/G)  gene  polymorphism  was widely studied  and associatedwith hypertension and other vascular disease. Until now, there wereno studies concerning role of Ang II receptor polymorphisms in MS.This study  suggest  possible  role  of  AT2R in  MS.  Further  studies areneeded to elucidate this result.",
journal = "Multiple Sclerosis Journal",
title = "Angiotensin II receptor type 2 (AT2R) -1332 A/G gene polymorphism as a risk factor for multiple sclerosis",
volume = "16",
pages = "S80-S81",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12791"
}

Kolaković, A. P., Živković, M., Dinčić, E., Popović, S., Raičević, R., Alavantić, D.,& Stanković, A.. (2010). Angiotensin II receptor type 2 (AT2R) -1332 A/G gene polymorphism as a risk factor for multiple sclerosis. in Multiple Sclerosis Journal, 16, S80-S81.
https://hdl.handle.net/21.15107/rcub_vinar_12791

Kolaković AP, Živković M, Dinčić E, Popović S, Raičević R, Alavantić D, Stanković A. Angiotensin II receptor type 2 (AT2R) -1332 A/G gene polymorphism as a risk factor for multiple sclerosis. in Multiple Sclerosis Journal. 2010;16:S80-S81.
https://hdl.handle.net/21.15107/rcub_vinar_12791 .

Kolaković, Ana P., Živković, Maja, Dinčić, Evica, Popović, Smiljana, Raičević, Ranko, Alavantić, Dragan, Stanković, Aleksandra, "Angiotensin II receptor type 2 (AT2R) -1332 A/G gene polymorphism as a risk factor for multiple sclerosis" in Multiple Sclerosis Journal, 16 (2010):S80-S81,
https://hdl.handle.net/21.15107/rcub_vinar_12791 .

TY  - CONF
AU  - Stanković, A.
AU  - Đurđević, V.
AU  - Kolaković, Ana
AU  - Stojković, Lj.
AU  - Živković, M.
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12699
AB  - The effects of environmental exposures might be transmitted to cellular level through mechanisms that could vary between individuals. Gene polymorphism in GSTM1/GSTT1, both deletions in the gene, showed the significant influence in genetic association studies of air pollution. In order to analyze the association of GSTT1/GSTM1 polymorphism with respiratory and cardiovascular disease in regard to air- pollution, for the first time we detected the presence of GSTM1/GSTT1 deletion in two different environmental risk factors' populations, Belgrade and Obrenovac. The groups consisted of 71 (Belgrade)/89 (Obrenovac) participants. Genotyping was performed by multiplex PCR. The frequencies of “null” genotypes for GSTM1/GSTT1 were not significantly different between the two investigated groups (47.89%/44.94%, 15.49%/17.98%, respectively). In conclusion, the frequencies of detected “null” genotypes in investigated two population subsets of Serbian origin was similar and in range with the frequencies in other Caucasian populations.
PB  - NILU - Norwegian Institute for Air Research
C3  - 2nd International WeBIOPATR Workshop : "Particulate Matter-Research and Management" : Proceedings
T1  - The importance of population susceptibility to air pollution: gene candidate approach
SP  - 38
EP  - 42
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12699
ER  - 

@conference{
author = "Stanković, A. and Đurđević, V. and Kolaković, Ana and Stojković, Lj. and Živković, M.",
year = "2009",
abstract = "The effects of environmental exposures might be transmitted to cellular level through mechanisms that could vary between individuals. Gene polymorphism in GSTM1/GSTT1, both deletions in the gene, showed the significant influence in genetic association studies of air pollution. In order to analyze the association of GSTT1/GSTM1 polymorphism with respiratory and cardiovascular disease in regard to air- pollution, for the first time we detected the presence of GSTM1/GSTT1 deletion in two different environmental risk factors' populations, Belgrade and Obrenovac. The groups consisted of 71 (Belgrade)/89 (Obrenovac) participants. Genotyping was performed by multiplex PCR. The frequencies of “null” genotypes for GSTM1/GSTT1 were not significantly different between the two investigated groups (47.89%/44.94%, 15.49%/17.98%, respectively). In conclusion, the frequencies of detected “null” genotypes in investigated two population subsets of Serbian origin was similar and in range with the frequencies in other Caucasian populations.",
publisher = "NILU - Norwegian Institute for Air Research",
journal = "2nd International WeBIOPATR Workshop : "Particulate Matter-Research and Management" : Proceedings",
title = "The importance of population susceptibility to air pollution: gene candidate approach",
pages = "38-42",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12699"
}

Stanković, A., Đurđević, V., Kolaković, A., Stojković, Lj.,& Živković, M.. (2009). The importance of population susceptibility to air pollution: gene candidate approach. in 2nd International WeBIOPATR Workshop : "Particulate Matter-Research and Management" : Proceedings
NILU - Norwegian Institute for Air Research., 38-42.
https://hdl.handle.net/21.15107/rcub_vinar_12699

Stanković A, Đurđević V, Kolaković A, Stojković L, Živković M. The importance of population susceptibility to air pollution: gene candidate approach. in 2nd International WeBIOPATR Workshop : "Particulate Matter-Research and Management" : Proceedings. 2009;:38-42.
https://hdl.handle.net/21.15107/rcub_vinar_12699 .

Stanković, A., Đurđević, V., Kolaković, Ana, Stojković, Lj., Živković, M., "The importance of population susceptibility to air pollution: gene candidate approach" in 2nd International WeBIOPATR Workshop : "Particulate Matter-Research and Management" : Proceedings (2009):38-42,
https://hdl.handle.net/21.15107/rcub_vinar_12699 .