TY  - JOUR
AU  - Gogoi, Roktim
AU  - Begum, Twahira
AU  - Sarma, Neelav
AU  - Tamang, Raghu
AU  - Chanda, Sanjoy K.
AU  - Lal, Mohan
AU  - Perveen, Kahkashan
AU  - Khan, Faheema
AU  - Marinković, Jelena
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13099
AB  - Pelargonium graveolens L., essential oil (EO) has various therapeutic properties, including aromatherapy. The present experiment was designed to investigate chemical makeup of the P. graveolens (Geranium) EO from Northeast India using gas chromatography-mass spectroscopy (GC-MS) and gas chromatography-flame ionization detection (GC-FID), and the pharmacological potential was determined using a range of assays along with Allium cepa assay for genotoxicity study. GC-MS analysis of the EO revealed the presence of 11 compounds with a total 97.43% identified area, with citronellol (32.84%), geraniol (23.62%), and citronellyl formate (12.14%) as the major compounds. The essential oil exhibited type-II anti-diabetic properties with an IC50 6.66 μL/mL and skin whitening potential IC50 of 1.89 μL/mL. P. graveolens EO (IC50 26.16 μL/mL) was found to possess comparatively weaker anti-inflammatory agent than the standard diclofenac (IC50 9.24 μL/mL) in the protease inhibitory test. The EO demonstrated moderate antioxidant activity with an IC50 value of 22.68 μL/mL than ascorbic acid IC50 12.53 μL/mL in the ABTS assay. The EO was found to moderately affect the mitotic index (15.32%) and induce chromosomal aberrations (11.40%) at a concentration of 1 μL/mL after 72 h of exposure. Based on the findings the Northeast Indian variety of P. graveolens EO exhibits promising type-II anti-diabetic and skin whitening properties, which could be further utilized in the formulation of anti-diabetic and skin whitening agents after validation through clinical trials.
T2  - Journal of Essential Oil Bearing Plants
T1  - Pelargonium graveolens L., (Geranium) essential oil from Northeast India: chemical composition, pharmacology and genotoxicity study
VL  - 27
IS  - 1
SP  - 135
EP  - 151
DO  - 10.1080/0972060X.2024.2311825
ER  - 

@article{
author = "Gogoi, Roktim and Begum, Twahira and Sarma, Neelav and Tamang, Raghu and Chanda, Sanjoy K. and Lal, Mohan and Perveen, Kahkashan and Khan, Faheema and Marinković, Jelena",
year = "2024",
abstract = "Pelargonium graveolens L., essential oil (EO) has various therapeutic properties, including aromatherapy. The present experiment was designed to investigate chemical makeup of the P. graveolens (Geranium) EO from Northeast India using gas chromatography-mass spectroscopy (GC-MS) and gas chromatography-flame ionization detection (GC-FID), and the pharmacological potential was determined using a range of assays along with Allium cepa assay for genotoxicity study. GC-MS analysis of the EO revealed the presence of 11 compounds with a total 97.43% identified area, with citronellol (32.84%), geraniol (23.62%), and citronellyl formate (12.14%) as the major compounds. The essential oil exhibited type-II anti-diabetic properties with an IC50 6.66 μL/mL and skin whitening potential IC50 of 1.89 μL/mL. P. graveolens EO (IC50 26.16 μL/mL) was found to possess comparatively weaker anti-inflammatory agent than the standard diclofenac (IC50 9.24 μL/mL) in the protease inhibitory test. The EO demonstrated moderate antioxidant activity with an IC50 value of 22.68 μL/mL than ascorbic acid IC50 12.53 μL/mL in the ABTS assay. The EO was found to moderately affect the mitotic index (15.32%) and induce chromosomal aberrations (11.40%) at a concentration of 1 μL/mL after 72 h of exposure. Based on the findings the Northeast Indian variety of P. graveolens EO exhibits promising type-II anti-diabetic and skin whitening properties, which could be further utilized in the formulation of anti-diabetic and skin whitening agents after validation through clinical trials.",
journal = "Journal of Essential Oil Bearing Plants",
title = "Pelargonium graveolens L., (Geranium) essential oil from Northeast India: chemical composition, pharmacology and genotoxicity study",
volume = "27",
number = "1",
pages = "135-151",
doi = "10.1080/0972060X.2024.2311825"
}

Gogoi, R., Begum, T., Sarma, N., Tamang, R., Chanda, S. K., Lal, M., Perveen, K., Khan, F.,& Marinković, J.. (2024). Pelargonium graveolens L., (Geranium) essential oil from Northeast India: chemical composition, pharmacology and genotoxicity study. in Journal of Essential Oil Bearing Plants, 27(1), 135-151.
https://doi.org/10.1080/0972060X.2024.2311825

Gogoi R, Begum T, Sarma N, Tamang R, Chanda SK, Lal M, Perveen K, Khan F, Marinković J. Pelargonium graveolens L., (Geranium) essential oil from Northeast India: chemical composition, pharmacology and genotoxicity study. in Journal of Essential Oil Bearing Plants. 2024;27(1):135-151.
doi:10.1080/0972060X.2024.2311825 .

Gogoi, Roktim, Begum, Twahira, Sarma, Neelav, Tamang, Raghu, Chanda, Sanjoy K., Lal, Mohan, Perveen, Kahkashan, Khan, Faheema, Marinković, Jelena, "Pelargonium graveolens L., (Geranium) essential oil from Northeast India: chemical composition, pharmacology and genotoxicity study" in Journal of Essential Oil Bearing Plants, 27, no. 1 (2024):135-151,
https://doi.org/10.1080/0972060X.2024.2311825 . .

TY  - JOUR
AU  - Rakašević, Dragana
AU  - Marinković, Jelena
AU  - Rakonjac, Bojan
AU  - Arce, Marion
AU  - Joksimović, Ena
AU  - Marković, Jovana
AU  - Kulić, Milan
AU  - Hadži-Mihailović, Miloš
AU  - Marković, Aleksa
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12444
AB  - Background: The long-term use of antifungal therapy in denture stomatitis (DS) treatment could be accompanied by antifungal-resistant strain onset, leading to compromised therapeutic procedure and disease reappearance. Photodynamic therapy (PDT) has shown the ability to eradicate oral infections and resistance strains. This prospective clinical study aimed to assess the PDT’s effectiveness compared to the conventional treatment on clinical and microbiological parameters in patients with DS without denture wear during the treatment and follow-ups. Methods: Forty-two patients diagnosed with DS were randomly assigned to one-session single PDT application (test group) or conventional antifungal therapy (control group). Clinical and microbiological parameters were assessed and analyzed before and at 3rd, 15th, and 30th day following the treatments. Microbiological samples were analyzed by a Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The data was statistically analyzed. Results: Prior to the treatment, Candida species, including C. albicans (100%), C. glabrata (33%), C. tropicalis (31%), C. krusei (31%) were isolated in all patients. Both treatment procedures demonstrated a statistically significant reduction in C. albicans at all follow-up time intervals (p < 0.05). However, PDT displayed a statistically significant reduction in C. krusei compared to the conventional treatment at all follow-up periods (p < 0.05). Clinical parameters improved considerably in the test group compared to the control group at the 3rd and 15th day of follow-up. Conclusion: One-session single PDT application demonstrated significant improvement in both clinical and microbiological outcomes in a short-term period, resulting in complete Candida spp. eradication compared to conventional antifungal therapy.
T2  - Photodiagnosis and Photodynamic Therapy
T1  - Evaluation of photodynamic therapy efficacy vs. conventional antifungal therapy in patients with poor-fitting dentures suffering from denture stomatitis. A prospective clinical study
VL  - 45
SP  - 103913
DO  - 10.1016/j.pdpdt.2023.103913
ER  - 

@article{
author = "Rakašević, Dragana and Marinković, Jelena and Rakonjac, Bojan and Arce, Marion and Joksimović, Ena and Marković, Jovana and Kulić, Milan and Hadži-Mihailović, Miloš and Marković, Aleksa",
year = "2024",
abstract = "Background: The long-term use of antifungal therapy in denture stomatitis (DS) treatment could be accompanied by antifungal-resistant strain onset, leading to compromised therapeutic procedure and disease reappearance. Photodynamic therapy (PDT) has shown the ability to eradicate oral infections and resistance strains. This prospective clinical study aimed to assess the PDT’s effectiveness compared to the conventional treatment on clinical and microbiological parameters in patients with DS without denture wear during the treatment and follow-ups. Methods: Forty-two patients diagnosed with DS were randomly assigned to one-session single PDT application (test group) or conventional antifungal therapy (control group). Clinical and microbiological parameters were assessed and analyzed before and at 3rd, 15th, and 30th day following the treatments. Microbiological samples were analyzed by a Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The data was statistically analyzed. Results: Prior to the treatment, Candida species, including C. albicans (100%), C. glabrata (33%), C. tropicalis (31%), C. krusei (31%) were isolated in all patients. Both treatment procedures demonstrated a statistically significant reduction in C. albicans at all follow-up time intervals (p < 0.05). However, PDT displayed a statistically significant reduction in C. krusei compared to the conventional treatment at all follow-up periods (p < 0.05). Clinical parameters improved considerably in the test group compared to the control group at the 3rd and 15th day of follow-up. Conclusion: One-session single PDT application demonstrated significant improvement in both clinical and microbiological outcomes in a short-term period, resulting in complete Candida spp. eradication compared to conventional antifungal therapy.",
journal = "Photodiagnosis and Photodynamic Therapy",
title = "Evaluation of photodynamic therapy efficacy vs. conventional antifungal therapy in patients with poor-fitting dentures suffering from denture stomatitis. A prospective clinical study",
volume = "45",
pages = "103913",
doi = "10.1016/j.pdpdt.2023.103913"
}

Rakašević, D., Marinković, J., Rakonjac, B., Arce, M., Joksimović, E., Marković, J., Kulić, M., Hadži-Mihailović, M.,& Marković, A.. (2024). Evaluation of photodynamic therapy efficacy vs. conventional antifungal therapy in patients with poor-fitting dentures suffering from denture stomatitis. A prospective clinical study. in Photodiagnosis and Photodynamic Therapy, 45, 103913.
https://doi.org/10.1016/j.pdpdt.2023.103913

Rakašević D, Marinković J, Rakonjac B, Arce M, Joksimović E, Marković J, Kulić M, Hadži-Mihailović M, Marković A. Evaluation of photodynamic therapy efficacy vs. conventional antifungal therapy in patients with poor-fitting dentures suffering from denture stomatitis. A prospective clinical study. in Photodiagnosis and Photodynamic Therapy. 2024;45:103913.
doi:10.1016/j.pdpdt.2023.103913 .

Rakašević, Dragana, Marinković, Jelena, Rakonjac, Bojan, Arce, Marion, Joksimović, Ena, Marković, Jovana, Kulić, Milan, Hadži-Mihailović, Miloš, Marković, Aleksa, "Evaluation of photodynamic therapy efficacy vs. conventional antifungal therapy in patients with poor-fitting dentures suffering from denture stomatitis. A prospective clinical study" in Photodiagnosis and Photodynamic Therapy, 45 (2024):103913,
https://doi.org/10.1016/j.pdpdt.2023.103913 . .

TY  - JOUR
AU  - Petrović, Jelena
AU  - Marinković, Jelena
AU  - Živković, Sanja
AU  - Radenković, Marina
AU  - Ciganović, Jovan
AU  - Marković, Dejan
AU  - Momčilović, Miloš
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11369
AB  - The purpose of this study is to evaluate the feasibility of using the originally developed Laser Induced Breakdown Spectroscopy (LIBS) laboratory setup for the qualitative analysis of human tooth tissues and tooth restorations. Plasma was induced by the infrared TEA (Transversally Excited Atmospheric) CO2 laser in the air under atmospheric pressure. Optical emission spectra with sharp and well-resolved spectral lines and a negligibly low background were obtained. The results of the qualitative analysis showed the presence of various elements in the tooth tissues (P, Ca, and Mg), as well as trace elements within restorations such as Zn, Sn, Cu, Ag, and Hg. The estimated temperatures of plasma for composite, dentin, and amalgam were 21,000 K, 16,200 K, and 13,300 K respectively. The electron density deduced from the Stark-broadening line profiles varies from 8.5 × 1017 cm− 3 for amalgam, 2 × 1018 cm− 3 for composite, and 1.9 × 1018 cm− 3 for dentin. The surface of the tooth tissue was imaged with a scanning electron microscope (SEM), showing that dentin contains pores, cavities and bubbles. The results indicated that the alternative and cost-effective LIBS system can be used as a useful tool for further research on laser-induced plasma in dentistry. These insights are useful in the field of laser dentistry, where lasers are commonly utilized to treat teeth. © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
T2  - Plasma Chemistry and Plasma Processing
T1  - The TEA CO2 Laser as a Promising tool for Elemental Analysis of Restored Teeth and Evaluation of Restoration Constituent Penetration into Dental Tissues
DO  - 10.1007/s11090-023-10348-5
ER  - 

@article{
author = "Petrović, Jelena and Marinković, Jelena and Živković, Sanja and Radenković, Marina and Ciganović, Jovan and Marković, Dejan and Momčilović, Miloš",
year = "2023",
abstract = "The purpose of this study is to evaluate the feasibility of using the originally developed Laser Induced Breakdown Spectroscopy (LIBS) laboratory setup for the qualitative analysis of human tooth tissues and tooth restorations. Plasma was induced by the infrared TEA (Transversally Excited Atmospheric) CO2 laser in the air under atmospheric pressure. Optical emission spectra with sharp and well-resolved spectral lines and a negligibly low background were obtained. The results of the qualitative analysis showed the presence of various elements in the tooth tissues (P, Ca, and Mg), as well as trace elements within restorations such as Zn, Sn, Cu, Ag, and Hg. The estimated temperatures of plasma for composite, dentin, and amalgam were 21,000 K, 16,200 K, and 13,300 K respectively. The electron density deduced from the Stark-broadening line profiles varies from 8.5 × 1017 cm− 3 for amalgam, 2 × 1018 cm− 3 for composite, and 1.9 × 1018 cm− 3 for dentin. The surface of the tooth tissue was imaged with a scanning electron microscope (SEM), showing that dentin contains pores, cavities and bubbles. The results indicated that the alternative and cost-effective LIBS system can be used as a useful tool for further research on laser-induced plasma in dentistry. These insights are useful in the field of laser dentistry, where lasers are commonly utilized to treat teeth. © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.",
journal = "Plasma Chemistry and Plasma Processing",
title = "The TEA CO2 Laser as a Promising tool for Elemental Analysis of Restored Teeth and Evaluation of Restoration Constituent Penetration into Dental Tissues",
doi = "10.1007/s11090-023-10348-5"
}

Petrović, J., Marinković, J., Živković, S., Radenković, M., Ciganović, J., Marković, D.,& Momčilović, M.. (2023). The TEA CO2 Laser as a Promising tool for Elemental Analysis of Restored Teeth and Evaluation of Restoration Constituent Penetration into Dental Tissues. in Plasma Chemistry and Plasma Processing.
https://doi.org/10.1007/s11090-023-10348-5

Petrović J, Marinković J, Živković S, Radenković M, Ciganović J, Marković D, Momčilović M. The TEA CO2 Laser as a Promising tool for Elemental Analysis of Restored Teeth and Evaluation of Restoration Constituent Penetration into Dental Tissues. in Plasma Chemistry and Plasma Processing. 2023;.
doi:10.1007/s11090-023-10348-5 .

Petrović, Jelena, Marinković, Jelena, Živković, Sanja, Radenković, Marina, Ciganović, Jovan, Marković, Dejan, Momčilović, Miloš, "The TEA CO2 Laser as a Promising tool for Elemental Analysis of Restored Teeth and Evaluation of Restoration Constituent Penetration into Dental Tissues" in Plasma Chemistry and Plasma Processing (2023),
https://doi.org/10.1007/s11090-023-10348-5 . .

TY  - CONF
AU  - Petrović, Jelena D.
AU  - Marinković, Jelena
AU  - Radenković, Marina
AU  - Živković, Sanja
AU  - Ciganović, Jovan
AU  - Momčilović, Miloš
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12438
AB  - У овом раду се испитује примена спектроскопије ласерски индуковане плазме (LIBS) за анализу елементног састава зуба и пломбе. Коришћењем CO2 ласера као извора побуђивања, добијени су емисиони спектри са оштрим спектралним линијама и ниском позадином који су омогућили идентификацију елементног састава ткива зуба и пломбе. Испитивани су и параметри плазме, температура и густина електрона, што је важно за разумевање процеса током LIBS анализе. Добијени резултати показују да LIBS техника са CO2 ласером може пружити корисне информације о елементном саставу зуба и пломбе, што је значајно за стоматологију, археологију и форензику [1].
AB  - This study investigates the application of laser-induced breakdown spectroscopy (LIBS) for analyzing the elemental composition of teeth and dental fillings. Using a CO2 laser as the excitation source, emission spectra with sharp spectral lines and low background were obtained, allowing for the identification of the elemental composition of tooth and filling tissue. Plasma parameters, including electron temperature and density, were also examined, which is important for understanding the process during LIBS analysis. The results show that the LIBS technique with a CO2 laser can provide useful information on the elemental composition of teeth and fillings, which is significant for dentistry, archaeology, and forensics [1].
PB  - Belgrade : Serbian Chemical Society
C3  - 59th Meeting of the Serbian Chemical Society : Book of Abstracts, Proceedings
T1  - Елементна анализа и дијагностика плазме хуманих зуба применом LIBS-a
T1  - Elemental analysis and plasma diagnostics of human teeth using LIBS
SP  - 118
EP  - 118
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12438
ER  - 

@conference{
author = "Petrović, Jelena D. and Marinković, Jelena and Radenković, Marina and Živković, Sanja and Ciganović, Jovan and Momčilović, Miloš",
year = "2023",
abstract = "У овом раду се испитује примена спектроскопије ласерски индуковане плазме (LIBS) за анализу елементног састава зуба и пломбе. Коришћењем CO2 ласера као извора побуђивања, добијени су емисиони спектри са оштрим спектралним линијама и ниском позадином који су омогућили идентификацију елементног састава ткива зуба и пломбе. Испитивани су и параметри плазме, температура и густина електрона, што је важно за разумевање процеса током LIBS анализе. Добијени резултати показују да LIBS техника са CO2 ласером може пружити корисне информације о елементном саставу зуба и пломбе, што је значајно за стоматологију, археологију и форензику [1]., This study investigates the application of laser-induced breakdown spectroscopy (LIBS) for analyzing the elemental composition of teeth and dental fillings. Using a CO2 laser as the excitation source, emission spectra with sharp spectral lines and low background were obtained, allowing for the identification of the elemental composition of tooth and filling tissue. Plasma parameters, including electron temperature and density, were also examined, which is important for understanding the process during LIBS analysis. The results show that the LIBS technique with a CO2 laser can provide useful information on the elemental composition of teeth and fillings, which is significant for dentistry, archaeology, and forensics [1].",
publisher = "Belgrade : Serbian Chemical Society",
journal = "59th Meeting of the Serbian Chemical Society : Book of Abstracts, Proceedings",
title = "Елементна анализа и дијагностика плазме хуманих зуба применом LIBS-a, Elemental analysis and plasma diagnostics of human teeth using LIBS",
pages = "118-118",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12438"
}

Petrović, J. D., Marinković, J., Radenković, M., Živković, S., Ciganović, J.,& Momčilović, M.. (2023). Елементна анализа и дијагностика плазме хуманих зуба применом LIBS-a. in 59th Meeting of the Serbian Chemical Society : Book of Abstracts, Proceedings
Belgrade : Serbian Chemical Society., 118-118.
https://hdl.handle.net/21.15107/rcub_vinar_12438

Petrović JD, Marinković J, Radenković M, Živković S, Ciganović J, Momčilović M. Елементна анализа и дијагностика плазме хуманих зуба применом LIBS-a. in 59th Meeting of the Serbian Chemical Society : Book of Abstracts, Proceedings. 2023;:118-118.
https://hdl.handle.net/21.15107/rcub_vinar_12438 .

Petrović, Jelena D., Marinković, Jelena, Radenković, Marina, Živković, Sanja, Ciganović, Jovan, Momčilović, Miloš, "Елементна анализа и дијагностика плазме хуманих зуба применом LIBS-a" in 59th Meeting of the Serbian Chemical Society : Book of Abstracts, Proceedings (2023):118-118,
https://hdl.handle.net/21.15107/rcub_vinar_12438 .

TY  - CONF
AU  - Nemoda, Milica
AU  - Nikolić, Biljana
AU  - Momčilović, Miloš
AU  - Živković, Sanja
AU  - Marković, Dejan
AU  - Živković, Lada
AU  - Marinković, Jelena
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13033
AB  - Cilj istraživanja bio je ispitivanje antimikrobnog potencijala monoterpena karvakrola i geraniola na bakterijskim izolatima Streptococcus spp. od značaja za etiologiju karijesa (S. mutans, S. sanguinis, S. parasanguinis, S. gordonii). Antimikrobni potencijal karvakrola i geraniola testiran je mikrodilucionim testom, kojim su određene minimalna inhibitorna koncentracija (MIC) i minimalna baktericidna koncentracija (MBC). Testirani monoterpeni ispoljili su značajan antimikrobni potencijal, MIC je bio u opsegu od 0.55 mg/mL do 4.39 mg/mL, a MBC od 2.20 mg/mL do 19.54 mg/mL.
AB  - The aim of our study was to investigate the antimicrobial potential of monoterpenes carvacrol and geraniol towards the Streptococcus spp. isolates relevant to tooth caries (S. mutans, S. sanguinis, S. parasanguinis, S gordonii). Microdilution test was used for the assessment of the antimicrobial potential of carvacrol and geraniol, and the minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) were determined. Tested monoterpenes achieved notable antimicrobial activity, with MICs ranging from 0.55 mg/mL to 4.39 mg/mL, and MBCs from 2.20 mg/mL to 19.54 mg/mL.
PB  - Belgrade : Serbian Chemical Society
C3  - 59th Meeting of the Serbian Chemical Society : Book of Abstracts, Proceedings
T1  - Potencijal monoterpena karvakrola i geraniola u inhibiciji oralnih patogena
T1  - Antibacterial potential of the monoterpenes carvacrol and geraniol against oral pathogens
SP  - 73
EP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_vinar_13033
ER  - 

@conference{
author = "Nemoda, Milica and Nikolić, Biljana and Momčilović, Miloš and Živković, Sanja and Marković, Dejan and Živković, Lada and Marinković, Jelena",
year = "2023",
abstract = "Cilj istraživanja bio je ispitivanje antimikrobnog potencijala monoterpena karvakrola i geraniola na bakterijskim izolatima Streptococcus spp. od značaja za etiologiju karijesa (S. mutans, S. sanguinis, S. parasanguinis, S. gordonii). Antimikrobni potencijal karvakrola i geraniola testiran je mikrodilucionim testom, kojim su određene minimalna inhibitorna koncentracija (MIC) i minimalna baktericidna koncentracija (MBC). Testirani monoterpeni ispoljili su značajan antimikrobni potencijal, MIC je bio u opsegu od 0.55 mg/mL do 4.39 mg/mL, a MBC od 2.20 mg/mL do 19.54 mg/mL., The aim of our study was to investigate the antimicrobial potential of monoterpenes carvacrol and geraniol towards the Streptococcus spp. isolates relevant to tooth caries (S. mutans, S. sanguinis, S. parasanguinis, S gordonii). Microdilution test was used for the assessment of the antimicrobial potential of carvacrol and geraniol, and the minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) were determined. Tested monoterpenes achieved notable antimicrobial activity, with MICs ranging from 0.55 mg/mL to 4.39 mg/mL, and MBCs from 2.20 mg/mL to 19.54 mg/mL.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "59th Meeting of the Serbian Chemical Society : Book of Abstracts, Proceedings",
title = "Potencijal monoterpena karvakrola i geraniola u inhibiciji oralnih patogena, Antibacterial potential of the monoterpenes carvacrol and geraniol against oral pathogens",
pages = "73-73",
url = "https://hdl.handle.net/21.15107/rcub_vinar_13033"
}

Nemoda, M., Nikolić, B., Momčilović, M., Živković, S., Marković, D., Živković, L.,& Marinković, J.. (2023). Potencijal monoterpena karvakrola i geraniola u inhibiciji oralnih patogena. in 59th Meeting of the Serbian Chemical Society : Book of Abstracts, Proceedings
Belgrade : Serbian Chemical Society., 73-73.
https://hdl.handle.net/21.15107/rcub_vinar_13033

Nemoda M, Nikolić B, Momčilović M, Živković S, Marković D, Živković L, Marinković J. Potencijal monoterpena karvakrola i geraniola u inhibiciji oralnih patogena. in 59th Meeting of the Serbian Chemical Society : Book of Abstracts, Proceedings. 2023;:73-73.
https://hdl.handle.net/21.15107/rcub_vinar_13033 .

Nemoda, Milica, Nikolić, Biljana, Momčilović, Miloš, Živković, Sanja, Marković, Dejan, Živković, Lada, Marinković, Jelena, "Potencijal monoterpena karvakrola i geraniola u inhibiciji oralnih patogena" in 59th Meeting of the Serbian Chemical Society : Book of Abstracts, Proceedings (2023):73-73,
https://hdl.handle.net/21.15107/rcub_vinar_13033 .

TY  - JOUR
AU  - Marinković, Jelena
AU  - Rakašević, Dragana
AU  - Nemoda, Milica
AU  - Nikolić, Biljana
AU  - Marković, Tatjana
AU  - Matijević, Stevo
AU  - Marković, Dejan
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11670
AB  - The possibility to utilize the antibacterial and antibiofilm potential of essential oils (EOs) in dentistry is recognized and best seen in the design of EO-based mouthwashes. The up-to-date results, obtained with the most commonly used formulation being consisted of thymol, eucalyptol, menthol, and methyl salicylate (Listerine), proved its efficacy against plaque and gingival inflammation. However, novel alcohol-free products remain to be further investigated. Commercially available mouthwash containing curcumin, clove oil, mentha oil, eucalyptol, thymol and tea tree oil, and the mouthwash based on ginger EO (a-zingiberene, b-bisabolene, b-sesquiphellandrene, curcumene), reduced plaque index and improved periodontal status of the patients. The plaque index was also reduced and overall healing was improved after the surgical removal of the third molar by mouthwash containing John wort oil. In addition, Cinnamomum zeylanicum EO-based mouthwash and mouthwash containing peppermint oil were efficient against stomatitis and xerostomia, respectively. In addition to beneficial effects achieved in the mouth, i.e. at the appropriate site of application, novel investigations pointed-out that EO-based mouthwashes could be recognized as efficient in the reduction of bacterial and viral aerosols. This has been shown for Listerine, and for several mouthwashes containing following active substances of plants' EO origin: a-farnesene, b-farnesene, farnesol, nerolidol, a-bulnesene, eremanthin, b-sesquiphellandrene, spiroether, cinnamylacetate, cinnamaldehyde, eugenol, menthol, and carvacrol. This finding could be of special interest, in order to be included in further clinical studies related to COVID -19 pandemic.
T2  - Balkan Journal of Dental Medicine
T1  - EO-based mouthwashes: Is there something that should be known?
VL  - 27
IS  - 1
SP  - 32
EP  - 37
DO  - 10.5937/bjdm2301032M
ER  - 

@article{
author = "Marinković, Jelena and Rakašević, Dragana and Nemoda, Milica and Nikolić, Biljana and Marković, Tatjana and Matijević, Stevo and Marković, Dejan",
year = "2023",
abstract = "The possibility to utilize the antibacterial and antibiofilm potential of essential oils (EOs) in dentistry is recognized and best seen in the design of EO-based mouthwashes. The up-to-date results, obtained with the most commonly used formulation being consisted of thymol, eucalyptol, menthol, and methyl salicylate (Listerine), proved its efficacy against plaque and gingival inflammation. However, novel alcohol-free products remain to be further investigated. Commercially available mouthwash containing curcumin, clove oil, mentha oil, eucalyptol, thymol and tea tree oil, and the mouthwash based on ginger EO (a-zingiberene, b-bisabolene, b-sesquiphellandrene, curcumene), reduced plaque index and improved periodontal status of the patients. The plaque index was also reduced and overall healing was improved after the surgical removal of the third molar by mouthwash containing John wort oil. In addition, Cinnamomum zeylanicum EO-based mouthwash and mouthwash containing peppermint oil were efficient against stomatitis and xerostomia, respectively. In addition to beneficial effects achieved in the mouth, i.e. at the appropriate site of application, novel investigations pointed-out that EO-based mouthwashes could be recognized as efficient in the reduction of bacterial and viral aerosols. This has been shown for Listerine, and for several mouthwashes containing following active substances of plants' EO origin: a-farnesene, b-farnesene, farnesol, nerolidol, a-bulnesene, eremanthin, b-sesquiphellandrene, spiroether, cinnamylacetate, cinnamaldehyde, eugenol, menthol, and carvacrol. This finding could be of special interest, in order to be included in further clinical studies related to COVID -19 pandemic.",
journal = "Balkan Journal of Dental Medicine",
title = "EO-based mouthwashes: Is there something that should be known?",
volume = "27",
number = "1",
pages = "32-37",
doi = "10.5937/bjdm2301032M"
}

Marinković, J., Rakašević, D., Nemoda, M., Nikolić, B., Marković, T., Matijević, S.,& Marković, D.. (2023). EO-based mouthwashes: Is there something that should be known?. in Balkan Journal of Dental Medicine, 27(1), 32-37.
https://doi.org/10.5937/bjdm2301032M

Marinković J, Rakašević D, Nemoda M, Nikolić B, Marković T, Matijević S, Marković D. EO-based mouthwashes: Is there something that should be known?. in Balkan Journal of Dental Medicine. 2023;27(1):32-37.
doi:10.5937/bjdm2301032M .

Marinković, Jelena, Rakašević, Dragana, Nemoda, Milica, Nikolić, Biljana, Marković, Tatjana, Matijević, Stevo, Marković, Dejan, "EO-based mouthwashes: Is there something that should be known?" in Balkan Journal of Dental Medicine, 27, no. 1 (2023):32-37,
https://doi.org/10.5937/bjdm2301032M . .

TY  - JOUR
AU  - Rakašević, Dragana
AU  - Marinković, Jelena
AU  - Marković, Jovan
AU  - Nikolić, Biljana
AU  - Marković, Dejan
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11673
AB  - Background  The study aimed at determining antibacterial efficiency of adjuvant photodynamic therapy (PDT) and high-power diode laser (DL) in the treatment of chronic periapical periodontitis (CPP) in young permanent teeth. Methods  Forty-four young permanent teeth with CPP were randomly divided into three groups (PDT, DL and control). Each tooth underwent standard chemo-mechanical treatment, while within tested groups was additionally treated by PDT or DL. Bacterial identification and quantification were provided by MALDI–TOF spectrometry and plate counting assay, performed after accessing the canal, following chemo-mechanical preparation, and after PDT or DL procedure where applicable. Results  Thirty-nine young permanent teeth with CCP (patients age 9.77 ± 1.43) completed the study. Before the treatments, 202 isolates belonging to 13 genera/species, including Streptococcus (36), Actinomyces (34), Peptostreptococcus micros (27), Veillonella (25) and Enterococcus faecalis (22), were recovered. Chemo-mechanical treatment reduced CFU count in the all three groups (p < 0.001), but complete eradication was not observed for any of isolated species. Adjuvant PDT and DL completely eradicated isolates of 8 and 6 bacterial genera/species, resulting in complete bacterial elimination from 53.8% and 30.8% of root canals, respectively. In the rest canals, total Δlog CFUs were 4.71 and 4.58. Conclusion  The results indicated that both PDT and DL could be performed as adjuvants to standard endodontic treatment of the young permanent teeth with CPP.
T2  - Photodiagnosis and Photodynamic Therapy
T1  - Antibacterial efficiency of adjuvant photodynamic therapy and high-power diode laser in the treatment of young permanent teeth with chronic periapical periodontitis. A prospective clinical study
VL  - 41
SP  - 103129
DO  - 10.1016/j.pdpdt.2022.103129
ER  - 

@article{
author = "Rakašević, Dragana and Marinković, Jelena and Marković, Jovan and Nikolić, Biljana and Marković, Dejan",
year = "2023",
abstract = "Background  The study aimed at determining antibacterial efficiency of adjuvant photodynamic therapy (PDT) and high-power diode laser (DL) in the treatment of chronic periapical periodontitis (CPP) in young permanent teeth. Methods  Forty-four young permanent teeth with CPP were randomly divided into three groups (PDT, DL and control). Each tooth underwent standard chemo-mechanical treatment, while within tested groups was additionally treated by PDT or DL. Bacterial identification and quantification were provided by MALDI–TOF spectrometry and plate counting assay, performed after accessing the canal, following chemo-mechanical preparation, and after PDT or DL procedure where applicable. Results  Thirty-nine young permanent teeth with CCP (patients age 9.77 ± 1.43) completed the study. Before the treatments, 202 isolates belonging to 13 genera/species, including Streptococcus (36), Actinomyces (34), Peptostreptococcus micros (27), Veillonella (25) and Enterococcus faecalis (22), were recovered. Chemo-mechanical treatment reduced CFU count in the all three groups (p < 0.001), but complete eradication was not observed for any of isolated species. Adjuvant PDT and DL completely eradicated isolates of 8 and 6 bacterial genera/species, resulting in complete bacterial elimination from 53.8% and 30.8% of root canals, respectively. In the rest canals, total Δlog CFUs were 4.71 and 4.58. Conclusion  The results indicated that both PDT and DL could be performed as adjuvants to standard endodontic treatment of the young permanent teeth with CPP.",
journal = "Photodiagnosis and Photodynamic Therapy",
title = "Antibacterial efficiency of adjuvant photodynamic therapy and high-power diode laser in the treatment of young permanent teeth with chronic periapical periodontitis. A prospective clinical study",
volume = "41",
pages = "103129",
doi = "10.1016/j.pdpdt.2022.103129"
}

Rakašević, D., Marinković, J., Marković, J., Nikolić, B.,& Marković, D.. (2023). Antibacterial efficiency of adjuvant photodynamic therapy and high-power diode laser in the treatment of young permanent teeth with chronic periapical periodontitis. A prospective clinical study. in Photodiagnosis and Photodynamic Therapy, 41, 103129.
https://doi.org/10.1016/j.pdpdt.2022.103129

Rakašević D, Marinković J, Marković J, Nikolić B, Marković D. Antibacterial efficiency of adjuvant photodynamic therapy and high-power diode laser in the treatment of young permanent teeth with chronic periapical periodontitis. A prospective clinical study. in Photodiagnosis and Photodynamic Therapy. 2023;41:103129.
doi:10.1016/j.pdpdt.2022.103129 .

Rakašević, Dragana, Marinković, Jelena, Marković, Jovan, Nikolić, Biljana, Marković, Dejan, "Antibacterial efficiency of adjuvant photodynamic therapy and high-power diode laser in the treatment of young permanent teeth with chronic periapical periodontitis. A prospective clinical study" in Photodiagnosis and Photodynamic Therapy, 41 (2023):103129,
https://doi.org/10.1016/j.pdpdt.2022.103129 . .

TY  - CONF
AU  - Ilić, Bojana
AU  - Petrović, Božana
AU  - Marinković, Jelena
AU  - Filipović Tričković, Jelena
AU  - Miletić Vukajlović, Jadranka
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12728
AB  - Objectives: The main purpose of orthodontic treatment (OT) is to accomplish an optimal occlusal relationship in order to obtain adequate oral function and aesthetic appearance. Under optimal economic conditions, demand for OT reaches at least 35%, but in higher socioeconomic areas in US more than 50% of children are receiving orthodontic care. Acceptance of OT in Europe, like in western population and Scandinavian countries, is at similar levels. Very desirable mechanical characteristics, such as shape memory effect and superelasticity, expanded the use of nitinol (NiTi) archwires in orthodontics significantly. The complex conditions present in the oral cavity, including biofilm formation on the exposed surfaces, substantially alter the surface and structural properties of the NiTi archwires compromising the safety of OT. The necessity for developing novel material coating that would decrease Ni release and improve biological properties is of a great importance. Materials-Methods: Copper doped titanium nitride films (TiN-Cu) on the surface of NiTi archwires were obtained by combination of the cathodic arc evaporation and DC magnetron sputtering. The physicochemical characterization was performed using energy-dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD). Ion release was assessed using inductively coupled plasma optical emission spectrometry (ICP-OES). The cytotoxicity of NiTi archwires, stainless steel (SS) archwires and TiN-Cu coated archwires was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyl tetrazolium bromide (MTT) test. In order to evaluate bacterial adhesion and biofilm formation, the following strains were used: Streptococcus mutans and Streptococcus mitis. Results: Physicochemical characterization revealed well-designed coatings with the presence of TiN phase with incorporated Cu. The release of Ni was the lowest regarding TiN-Cunanocoated archwires (p < 0.05) and increased in acidic conditions, while the release of Ti was constant. Contrary, the release of Cu was higher in neutral conditions and decreased during the observation time (p < 0.05). The cytotoxicity was the lowest in 28-day eluates of TiN-Cu-nanocoated archwires (p < 0.05). The coating inhibited the adhesion and growth of bacteria such as Streptococcus mitis and Streptococcus mutans (p < 0.05). Conclusion: Taking into account the results of cytotoxicity test and biofilm formation, TiN-Cunanocoated archwires may be considered as a good candidate for further clinical investigations.
C3  - BaSS : 27th  International Balkan Congress Congress - “Past, Present and Future of Dental implants and Gerodontology“ : Book of abstracts
T1  - Biological properties of nitinol archwires coated with titanium nitride- copper films
SP  - 87
EP  - 87
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12728
ER  - 

@conference{
author = "Ilić, Bojana and Petrović, Božana and Marinković, Jelena and Filipović Tričković, Jelena and Miletić Vukajlović, Jadranka",
year = "2023",
abstract = "Objectives: The main purpose of orthodontic treatment (OT) is to accomplish an optimal occlusal relationship in order to obtain adequate oral function and aesthetic appearance. Under optimal economic conditions, demand for OT reaches at least 35%, but in higher socioeconomic areas in US more than 50% of children are receiving orthodontic care. Acceptance of OT in Europe, like in western population and Scandinavian countries, is at similar levels. Very desirable mechanical characteristics, such as shape memory effect and superelasticity, expanded the use of nitinol (NiTi) archwires in orthodontics significantly. The complex conditions present in the oral cavity, including biofilm formation on the exposed surfaces, substantially alter the surface and structural properties of the NiTi archwires compromising the safety of OT. The necessity for developing novel material coating that would decrease Ni release and improve biological properties is of a great importance. Materials-Methods: Copper doped titanium nitride films (TiN-Cu) on the surface of NiTi archwires were obtained by combination of the cathodic arc evaporation and DC magnetron sputtering. The physicochemical characterization was performed using energy-dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD). Ion release was assessed using inductively coupled plasma optical emission spectrometry (ICP-OES). The cytotoxicity of NiTi archwires, stainless steel (SS) archwires and TiN-Cu coated archwires was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyl tetrazolium bromide (MTT) test. In order to evaluate bacterial adhesion and biofilm formation, the following strains were used: Streptococcus mutans and Streptococcus mitis. Results: Physicochemical characterization revealed well-designed coatings with the presence of TiN phase with incorporated Cu. The release of Ni was the lowest regarding TiN-Cunanocoated archwires (p < 0.05) and increased in acidic conditions, while the release of Ti was constant. Contrary, the release of Cu was higher in neutral conditions and decreased during the observation time (p < 0.05). The cytotoxicity was the lowest in 28-day eluates of TiN-Cu-nanocoated archwires (p < 0.05). The coating inhibited the adhesion and growth of bacteria such as Streptococcus mitis and Streptococcus mutans (p < 0.05). Conclusion: Taking into account the results of cytotoxicity test and biofilm formation, TiN-Cunanocoated archwires may be considered as a good candidate for further clinical investigations.",
journal = "BaSS : 27th  International Balkan Congress Congress - “Past, Present and Future of Dental implants and Gerodontology“ : Book of abstracts",
title = "Biological properties of nitinol archwires coated with titanium nitride- copper films",
pages = "87-87",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12728"
}

Ilić, B., Petrović, B., Marinković, J., Filipović Tričković, J.,& Miletić Vukajlović, J.. (2023). Biological properties of nitinol archwires coated with titanium nitride- copper films. in BaSS : 27th  International Balkan Congress Congress - “Past, Present and Future of Dental implants and Gerodontology“ : Book of abstracts, 87-87.
https://hdl.handle.net/21.15107/rcub_vinar_12728

Ilić B, Petrović B, Marinković J, Filipović Tričković J, Miletić Vukajlović J. Biological properties of nitinol archwires coated with titanium nitride- copper films. in BaSS : 27th  International Balkan Congress Congress - “Past, Present and Future of Dental implants and Gerodontology“ : Book of abstracts. 2023;:87-87.
https://hdl.handle.net/21.15107/rcub_vinar_12728 .

Ilić, Bojana, Petrović, Božana, Marinković, Jelena, Filipović Tričković, Jelena, Miletić Vukajlović, Jadranka, "Biological properties of nitinol archwires coated with titanium nitride- copper films" in BaSS : 27th  International Balkan Congress Congress - “Past, Present and Future of Dental implants and Gerodontology“ : Book of abstracts (2023):87-87,
https://hdl.handle.net/21.15107/rcub_vinar_12728 .

TY  - JOUR
AU  - Ilić, Bojana
AU  - Petrović, Božana
AU  - Marinković, Jelena
AU  - Miletić Vukajlović, Jadranka
AU  - Stevanović, Momir
AU  - Potočnik, Jelena
AU  - Jokanović, Vukoman
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11624
AB  - Background: The use of nitinol (NiTi) archwires in orthodontic treatment has increased significantly due to unique mechanical properties. The greatest obstacle for safe orthodontic treatment is chemically or microbiologically induced corrosion, resulting in nickel (Ni) release. The aim of this investigation was to enhance corrosion resistance and introduce antibacterial properties to NiTi archwires by coating them with copper (Cu) doper titanium nitride (TiN-Cu). Methods: NiTi archwires were coated with TiN-Cu using cathodic arc evaporation (CAE) and direct current magnetron sputtering (DC-MS). The morphology of the sample was analyzed via field emission scanning electron microscopy (FESEM) and chemical composition was assessed using energy-dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and Fourier transformed infrared spectroscopy (FTIR). Inductively coupled plasma optical emission spectrometry (ICP-OES) was used to estimate the ion release. The biocompatibility of samples was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyl tetrazolium bromide (MTT) assay. Antibacterial activity was tested against Streptococcus mutans and Streptococcus mitis. Results: Physicochemical characterization revealed well-designed coatings with the presence of TiN phase with incorporated Cu. TiN-Cu-nanocoated archwires showed a statistically lower Ni release (p < 0.05). Relative cell viability was the highest in 28-day eluates of TiN-Cu-nanocoated archwires (p < 0.05). The most remarkable decrease in Streptococcus mitis concentrations was observed in the case of TiN-Cu-coated archwires (p < 0.05). Conclusion: Taking into account biocompatibility and antibacterial tests, TiN-Cu-nanocoated archwires may be considered as a good candidate for further clinical investigations
T2  - Coatings
T1  - Investigation of Ion Release and Antibacterial Properties of TiN-Cu-Nanocoated Nitinol Archwires
VL  - 13
IS  - 9
SP  - 1587
DO  - 10.3390/coatings13091587
ER  - 

@article{
author = "Ilić, Bojana and Petrović, Božana and Marinković, Jelena and Miletić Vukajlović, Jadranka and Stevanović, Momir and Potočnik, Jelena and Jokanović, Vukoman",
year = "2023",
abstract = "Background: The use of nitinol (NiTi) archwires in orthodontic treatment has increased significantly due to unique mechanical properties. The greatest obstacle for safe orthodontic treatment is chemically or microbiologically induced corrosion, resulting in nickel (Ni) release. The aim of this investigation was to enhance corrosion resistance and introduce antibacterial properties to NiTi archwires by coating them with copper (Cu) doper titanium nitride (TiN-Cu). Methods: NiTi archwires were coated with TiN-Cu using cathodic arc evaporation (CAE) and direct current magnetron sputtering (DC-MS). The morphology of the sample was analyzed via field emission scanning electron microscopy (FESEM) and chemical composition was assessed using energy-dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD) and Fourier transformed infrared spectroscopy (FTIR). Inductively coupled plasma optical emission spectrometry (ICP-OES) was used to estimate the ion release. The biocompatibility of samples was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyl tetrazolium bromide (MTT) assay. Antibacterial activity was tested against Streptococcus mutans and Streptococcus mitis. Results: Physicochemical characterization revealed well-designed coatings with the presence of TiN phase with incorporated Cu. TiN-Cu-nanocoated archwires showed a statistically lower Ni release (p < 0.05). Relative cell viability was the highest in 28-day eluates of TiN-Cu-nanocoated archwires (p < 0.05). The most remarkable decrease in Streptococcus mitis concentrations was observed in the case of TiN-Cu-coated archwires (p < 0.05). Conclusion: Taking into account biocompatibility and antibacterial tests, TiN-Cu-nanocoated archwires may be considered as a good candidate for further clinical investigations",
journal = "Coatings",
title = "Investigation of Ion Release and Antibacterial Properties of TiN-Cu-Nanocoated Nitinol Archwires",
volume = "13",
number = "9",
pages = "1587",
doi = "10.3390/coatings13091587"
}

Ilić, B., Petrović, B., Marinković, J., Miletić Vukajlović, J., Stevanović, M., Potočnik, J.,& Jokanović, V.. (2023). Investigation of Ion Release and Antibacterial Properties of TiN-Cu-Nanocoated Nitinol Archwires. in Coatings, 13(9), 1587.
https://doi.org/10.3390/coatings13091587

Ilić B, Petrović B, Marinković J, Miletić Vukajlović J, Stevanović M, Potočnik J, Jokanović V. Investigation of Ion Release and Antibacterial Properties of TiN-Cu-Nanocoated Nitinol Archwires. in Coatings. 2023;13(9):1587.
doi:10.3390/coatings13091587 .

Ilić, Bojana, Petrović, Božana, Marinković, Jelena, Miletić Vukajlović, Jadranka, Stevanović, Momir, Potočnik, Jelena, Jokanović, Vukoman, "Investigation of Ion Release and Antibacterial Properties of TiN-Cu-Nanocoated Nitinol Archwires" in Coatings, 13, no. 9 (2023):1587,
https://doi.org/10.3390/coatings13091587 . .

TY  - JOUR
AU  - Choubey, Vikash Kumar
AU  - Gupta, Tanmita
AU  - Gogoi, Roktim
AU  - Tamang, Raghu
AU  - Sarma, Neelav
AU  - Begum, Twahira
AU  - Lal, Mohan
AU  - Dutta, Dipanka
AU  - Perveen, Kahkashan
AU  - Khan, Faheema
AU  - Marinković, Jelena
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12048
AB  - Ocimum americanum L. is a highly valued aromatic plant whose leaves are used not only in traditional cuisine but also in the food, cosmetic, and pharmaceutical industries. This study aimed to identify the chemical profile of essential oil extracted from the leaves of Ocimum americanum by GC-FID and GC/MS grown in acidic soil conditions in the NE region of India. Furthermore, the biological activities like antioxidant, anti-diabetic, anti-inflammatory, and anti-tyrosinase activity were analyzed, as per standard methods. GC and GC/MS analysis identified 16 compounds in which citral-a (48.25%) was found as a major compound, followed by carveol (41.64%). The antioxidant activity was performed using DPPH and ABTS methods; and IC50 value obtained was 8.85 μL/mL and 28.82 μL/mL, respectively. The leaf essential oil of O. americanum exhibited strong anti-diabetic and anti-inflammatory activities, whereas moderate anti-tyrosinase activities with IC50 values of 40.84, 12.81, and 182.74 μL/mL, respectively. Furthermore, the ant-microbial activity was evaluated for four bacterial and four fungal strains, revealing that O. americanum essential oil expresses antifungal activity against S. cereviaceae only with a MIC value of 5±0.47 μg/mL. These evidences clearly indicates that essential oil obtained from the leaf of O. americanum showed potential anti-inflammatory and anti-diabetic activity that can be used as an alternative in pharmaceuticals and food industries as a natural source.
T2  - Journal of Essential Oil Bearing Plants
T1  - Chemical compositions and different biological activity of leaf essential oil of                    Ocimum americanum                    L. grown in acidic soil conditions
SP  - 1
EP  - 13
DO  - 10.1080/0972060X.2023.2278751
ER  - 

@article{
author = "Choubey, Vikash Kumar and Gupta, Tanmita and Gogoi, Roktim and Tamang, Raghu and Sarma, Neelav and Begum, Twahira and Lal, Mohan and Dutta, Dipanka and Perveen, Kahkashan and Khan, Faheema and Marinković, Jelena",
year = "2023",
abstract = "Ocimum americanum L. is a highly valued aromatic plant whose leaves are used not only in traditional cuisine but also in the food, cosmetic, and pharmaceutical industries. This study aimed to identify the chemical profile of essential oil extracted from the leaves of Ocimum americanum by GC-FID and GC/MS grown in acidic soil conditions in the NE region of India. Furthermore, the biological activities like antioxidant, anti-diabetic, anti-inflammatory, and anti-tyrosinase activity were analyzed, as per standard methods. GC and GC/MS analysis identified 16 compounds in which citral-a (48.25%) was found as a major compound, followed by carveol (41.64%). The antioxidant activity was performed using DPPH and ABTS methods; and IC50 value obtained was 8.85 μL/mL and 28.82 μL/mL, respectively. The leaf essential oil of O. americanum exhibited strong anti-diabetic and anti-inflammatory activities, whereas moderate anti-tyrosinase activities with IC50 values of 40.84, 12.81, and 182.74 μL/mL, respectively. Furthermore, the ant-microbial activity was evaluated for four bacterial and four fungal strains, revealing that O. americanum essential oil expresses antifungal activity against S. cereviaceae only with a MIC value of 5±0.47 μg/mL. These evidences clearly indicates that essential oil obtained from the leaf of O. americanum showed potential anti-inflammatory and anti-diabetic activity that can be used as an alternative in pharmaceuticals and food industries as a natural source.",
journal = "Journal of Essential Oil Bearing Plants",
title = "Chemical compositions and different biological activity of leaf essential oil of                    Ocimum americanum                    L. grown in acidic soil conditions",
pages = "1-13",
doi = "10.1080/0972060X.2023.2278751"
}

Choubey, V. K., Gupta, T., Gogoi, R., Tamang, R., Sarma, N., Begum, T., Lal, M., Dutta, D., Perveen, K., Khan, F.,& Marinković, J.. (2023). Chemical compositions and different biological activity of leaf essential oil of                    Ocimum americanum                    L. grown in acidic soil conditions. in Journal of Essential Oil Bearing Plants, 1-13.
https://doi.org/10.1080/0972060X.2023.2278751

Choubey VK, Gupta T, Gogoi R, Tamang R, Sarma N, Begum T, Lal M, Dutta D, Perveen K, Khan F, Marinković J. Chemical compositions and different biological activity of leaf essential oil of                    Ocimum americanum                    L. grown in acidic soil conditions. in Journal of Essential Oil Bearing Plants. 2023;:1-13.
doi:10.1080/0972060X.2023.2278751 .

Choubey, Vikash Kumar, Gupta, Tanmita, Gogoi, Roktim, Tamang, Raghu, Sarma, Neelav, Begum, Twahira, Lal, Mohan, Dutta, Dipanka, Perveen, Kahkashan, Khan, Faheema, Marinković, Jelena, "Chemical compositions and different biological activity of leaf essential oil of                    Ocimum americanum                    L. grown in acidic soil conditions" in Journal of Essential Oil Bearing Plants (2023):1-13,
https://doi.org/10.1080/0972060X.2023.2278751 . .

TY  - CONF
AU  - Petrović, Jelena D.
AU  - Marinković, Jelena
AU  - Živković, Sanja
AU  - Marković, Dejan
AU  - Momčilović, Miloš
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12751
AB  - This paper evaluates the possibilities of the original LIBS (Laser Induced Breakdown Spectroscopes) laboratory setup for the elementary analysis of human tooth tissues and tooth restorations. For that reason, samples of the teeth restored with amalgam and composite fillings were analyzed. In addition, the possibility of heavy metal diffusion from restoring materials into healthy tooth tissues was also examined. Plasma was induced by the TEA CO2 laser in the air under atmospheric pressure. Optical emission spectra with sharp and well-decomposed spectral lines and a negligibly low background were obtained. The results of the elemental analysis showed the presence of various trace elements in the tooth tissues (P, Ca, Mg) as well as within restorations such as Zn, Cu, Ag, Hg. The analysis also showed that cement successfully blocked diffusion from restoring materials (amalgam and composite) into the dentine below. Obtained preliminary results led to the conclusion that this alternative and cost-effective LIBS system can find application as a useful tool for further research on laser-induced plasma in dentistry
PB  - Belgrade : Society of Physical Chemists of Serbia
C3  - PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Book of abstracts
T1  - Application of LIBS technique for dental analysis
SP  - 31
EP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12751
ER  - 

@conference{
author = "Petrović, Jelena D. and Marinković, Jelena and Živković, Sanja and Marković, Dejan and Momčilović, Miloš",
year = "2022",
abstract = "This paper evaluates the possibilities of the original LIBS (Laser Induced Breakdown Spectroscopes) laboratory setup for the elementary analysis of human tooth tissues and tooth restorations. For that reason, samples of the teeth restored with amalgam and composite fillings were analyzed. In addition, the possibility of heavy metal diffusion from restoring materials into healthy tooth tissues was also examined. Plasma was induced by the TEA CO2 laser in the air under atmospheric pressure. Optical emission spectra with sharp and well-decomposed spectral lines and a negligibly low background were obtained. The results of the elemental analysis showed the presence of various trace elements in the tooth tissues (P, Ca, Mg) as well as within restorations such as Zn, Cu, Ag, Hg. The analysis also showed that cement successfully blocked diffusion from restoring materials (amalgam and composite) into the dentine below. Obtained preliminary results led to the conclusion that this alternative and cost-effective LIBS system can find application as a useful tool for further research on laser-induced plasma in dentistry",
publisher = "Belgrade : Society of Physical Chemists of Serbia",
journal = "PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Book of abstracts",
title = "Application of LIBS technique for dental analysis",
pages = "31-31",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12751"
}

Petrović, J. D., Marinković, J., Živković, S., Marković, D.,& Momčilović, M.. (2022). Application of LIBS technique for dental analysis. in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Book of abstracts
Belgrade : Society of Physical Chemists of Serbia., 31-31.
https://hdl.handle.net/21.15107/rcub_vinar_12751

Petrović JD, Marinković J, Živković S, Marković D, Momčilović M. Application of LIBS technique for dental analysis. in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Book of abstracts. 2022;:31-31.
https://hdl.handle.net/21.15107/rcub_vinar_12751 .

Petrović, Jelena D., Marinković, Jelena, Živković, Sanja, Marković, Dejan, Momčilović, Miloš, "Application of LIBS technique for dental analysis" in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Book of abstracts (2022):31-31,
https://hdl.handle.net/21.15107/rcub_vinar_12751 .

TY  - JOUR
AU  - Marinković, Jelena
AU  - Marković, Tatjana
AU  - Nikolić, Biljana
AU  - Ćirić, Ana
AU  - Mitić-Ćulafić, Dragana
AU  - Dukanović, Stefana
AU  - Krstić, Aleksandar D.
AU  - Pavlica, Dušan
AU  - Vlajić, Tamara
AU  - Marković, Dejan
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10224
AB  - The aim of the study was to investigate the potency of Cinnamomum camphora cineoliferum and Melaleuca ericifolia essential oils (EOs) against facultative and obligate anaerobes isolated from teeth’ infected root canals. Biocompatibility of the EOs was also in the study focus. Chemical composition of the EOs was determined by GC/MS analysis. Their antibacterial potential and cytotoxicity were evaluated by microdilution and MTT assays, respectively. Statistical analysis was performed by Student’s t-test and one way ANOVA. Two the most abundant constituents of C. camphora cineoliferum EO were 1,8-cineole and sabinene, while in M. ericifolia EO the dominant were linalool and 1,8-cineole. MICs achieved against all tested strains were in the ranges 0.95±0.50 - 22.50±0.00 mg/mL and 0.65±0.00 - 23.35±7.80 mg/mL for the C. camphora cioneoliferum and M. ericifolia EOs, respectively. Statistical analysis showed that response to both oils has not differed between facultative and obligate anaerobes. Sensitivity pattern of the tested strains was different for the EOs and the positive control triple antibiotic paste (TAP): while E. faecalis and certain strains of S. salivarius were among the most sensitive to the oils, they were the least susceptible to the conventional antimicrobial TAP. Although the observed cytotoxicity of the EOs after 30 min of cell exposition was high (50.78-66.35% and up to 70% for C. camphora cioneoliferum and M. ericifolia EOs, respectively) it was lower than that of positive control (1.5% NaOCl, p<0.05). To conclude, both EOs showed promising antibacterial potential and achieved at least the same or even better biocompatibility than the conventional intracanal antimicrobials. Obtained results strongly encourage future research with an aim to formulate C. camphora cioneoliferum and M. ericifolia EO- based irrigants and medicaments, that could be used in endodontic practice.
T2  - Journal of Essential Oil-Bearing Plants
T1  - Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes
VL  - 25
IS  - 1
SP  - 111
EP  - 125
DO  - 10.1080/0972060X.2022.2040386
ER  - 

@article{
author = "Marinković, Jelena and Marković, Tatjana and Nikolić, Biljana and Ćirić, Ana and Mitić-Ćulafić, Dragana and Dukanović, Stefana and Krstić, Aleksandar D. and Pavlica, Dušan and Vlajić, Tamara and Marković, Dejan",
year = "2022",
abstract = "The aim of the study was to investigate the potency of Cinnamomum camphora cineoliferum and Melaleuca ericifolia essential oils (EOs) against facultative and obligate anaerobes isolated from teeth’ infected root canals. Biocompatibility of the EOs was also in the study focus. Chemical composition of the EOs was determined by GC/MS analysis. Their antibacterial potential and cytotoxicity were evaluated by microdilution and MTT assays, respectively. Statistical analysis was performed by Student’s t-test and one way ANOVA. Two the most abundant constituents of C. camphora cineoliferum EO were 1,8-cineole and sabinene, while in M. ericifolia EO the dominant were linalool and 1,8-cineole. MICs achieved against all tested strains were in the ranges 0.95±0.50 - 22.50±0.00 mg/mL and 0.65±0.00 - 23.35±7.80 mg/mL for the C. camphora cioneoliferum and M. ericifolia EOs, respectively. Statistical analysis showed that response to both oils has not differed between facultative and obligate anaerobes. Sensitivity pattern of the tested strains was different for the EOs and the positive control triple antibiotic paste (TAP): while E. faecalis and certain strains of S. salivarius were among the most sensitive to the oils, they were the least susceptible to the conventional antimicrobial TAP. Although the observed cytotoxicity of the EOs after 30 min of cell exposition was high (50.78-66.35% and up to 70% for C. camphora cioneoliferum and M. ericifolia EOs, respectively) it was lower than that of positive control (1.5% NaOCl, p<0.05). To conclude, both EOs showed promising antibacterial potential and achieved at least the same or even better biocompatibility than the conventional intracanal antimicrobials. Obtained results strongly encourage future research with an aim to formulate C. camphora cioneoliferum and M. ericifolia EO- based irrigants and medicaments, that could be used in endodontic practice.",
journal = "Journal of Essential Oil-Bearing Plants",
title = "Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes",
volume = "25",
number = "1",
pages = "111-125",
doi = "10.1080/0972060X.2022.2040386"
}

Marinković, J., Marković, T., Nikolić, B., Ćirić, A., Mitić-Ćulafić, D., Dukanović, S., Krstić, A. D., Pavlica, D., Vlajić, T.,& Marković, D.. (2022). Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes. in Journal of Essential Oil-Bearing Plants, 25(1), 111-125.
https://doi.org/10.1080/0972060X.2022.2040386

Marinković J, Marković T, Nikolić B, Ćirić A, Mitić-Ćulafić D, Dukanović S, Krstić AD, Pavlica D, Vlajić T, Marković D. Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes. in Journal of Essential Oil-Bearing Plants. 2022;25(1):111-125.
doi:10.1080/0972060X.2022.2040386 .

Marinković, Jelena, Marković, Tatjana, Nikolić, Biljana, Ćirić, Ana, Mitić-Ćulafić, Dragana, Dukanović, Stefana, Krstić, Aleksandar D., Pavlica, Dušan, Vlajić, Tamara, Marković, Dejan, "Biocompatibility and Antibacterial Potential of the Cinnamomum camphora cineoliferum (L.) J. Presl. and Melaleuca ericifolia Sm. Essential Oils Against Facultative and Obligate Endodontic Anaerobes" in Journal of Essential Oil-Bearing Plants, 25, no. 1 (2022):111-125,
https://doi.org/10.1080/0972060X.2022.2040386 . .

TY  - JOUR
AU  - Marinković, Jelena
AU  - Bošković, Marko
AU  - Tasić, Gvozden
AU  - Vasilijević, Bojana
AU  - Marković, Dejan
AU  - Marković, Tatjana
AU  - Nikolić, Biljana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10396
AB  - Cymbopogon martinii is an aromatic crop cultivated for the production of its essential oil (EO) which has different applications. Bearing in mind that C. martinii EO (CMEO) has already been proved for high antibacterial potential the aim of this study was to formulate stable CMEO nanoemulsions, being capable to combat Enterococcus faecalis biofilm, especially within infected tooth’s root canal. Ten emulsions with variable CMEO content (2.5–10 % v/v) and Tween 80 (T80, 2.5–15 % v/v) were formulated (designated as ECMEO:T80) and screened for volume-weighted mean diameter (D4,3) and stability, using dynamic light scattering measurements. Chemical composition of stable nanoemulsions was monitored by UV–VIS and FTIR, while their antibacterial activity was estimated in microdilution assay. Antibiofilm properties of selected nanoemulsions were tested in vitro (cristal violet assay) and ex vivo (within root canals of the extracted teeth). Results showed that formulations containing 10 % of T80 and CMEO volume ≤ 6 %, as well as those having constant 2.5 % of CMEO and variable T80 volume (≤ 10%), namely E2.5:2.5, E2.5:5, E2.5:7.5, E2.5:10, E4:10, and E6:10, were stable and nanosized (D4,3 <100 nm). FTIR and UV–VIS analyses confirmed successful encapsulation of the CMEO by T80 and showed that nanoemulsification did not affect CMEO composition. Evaluation of antibacterial potential showed that the most efficient were E6:10, E2.5:2.5 and E4:10 (MIC values 0.37–1.97 mg mL−1). Analysis of dependency of determined MICs on mean diameter and CMEO volume showed that antibacterial potential increased with both parameters. E6:10, E2.5:2.5 and E4:10 also reduced in vitro biofilm (inhibitory range 17.6–58.4 %), as well as intracanal biofilm (reduction ~2Log CFU). Presented results suggest E6:10, E4:10 and E2.5:2.5 as the good candidates for further research.
T2  - Industrial Crops and Products
T1  - Cymbopogon martinii essential oil nanoemulsions: Physico-chemical characterization, antibacterial and antibiofilm potential against Enterococcus faecalis
VL  - 187
SP  - 115478
DO  - 10.1016/j.indcrop.2022.115478
ER  - 

@article{
author = "Marinković, Jelena and Bošković, Marko and Tasić, Gvozden and Vasilijević, Bojana and Marković, Dejan and Marković, Tatjana and Nikolić, Biljana",
year = "2022",
abstract = "Cymbopogon martinii is an aromatic crop cultivated for the production of its essential oil (EO) which has different applications. Bearing in mind that C. martinii EO (CMEO) has already been proved for high antibacterial potential the aim of this study was to formulate stable CMEO nanoemulsions, being capable to combat Enterococcus faecalis biofilm, especially within infected tooth’s root canal. Ten emulsions with variable CMEO content (2.5–10 % v/v) and Tween 80 (T80, 2.5–15 % v/v) were formulated (designated as ECMEO:T80) and screened for volume-weighted mean diameter (D4,3) and stability, using dynamic light scattering measurements. Chemical composition of stable nanoemulsions was monitored by UV–VIS and FTIR, while their antibacterial activity was estimated in microdilution assay. Antibiofilm properties of selected nanoemulsions were tested in vitro (cristal violet assay) and ex vivo (within root canals of the extracted teeth). Results showed that formulations containing 10 % of T80 and CMEO volume ≤ 6 %, as well as those having constant 2.5 % of CMEO and variable T80 volume (≤ 10%), namely E2.5:2.5, E2.5:5, E2.5:7.5, E2.5:10, E4:10, and E6:10, were stable and nanosized (D4,3 <100 nm). FTIR and UV–VIS analyses confirmed successful encapsulation of the CMEO by T80 and showed that nanoemulsification did not affect CMEO composition. Evaluation of antibacterial potential showed that the most efficient were E6:10, E2.5:2.5 and E4:10 (MIC values 0.37–1.97 mg mL−1). Analysis of dependency of determined MICs on mean diameter and CMEO volume showed that antibacterial potential increased with both parameters. E6:10, E2.5:2.5 and E4:10 also reduced in vitro biofilm (inhibitory range 17.6–58.4 %), as well as intracanal biofilm (reduction ~2Log CFU). Presented results suggest E6:10, E4:10 and E2.5:2.5 as the good candidates for further research.",
journal = "Industrial Crops and Products",
title = "Cymbopogon martinii essential oil nanoemulsions: Physico-chemical characterization, antibacterial and antibiofilm potential against Enterococcus faecalis",
volume = "187",
pages = "115478",
doi = "10.1016/j.indcrop.2022.115478"
}

Marinković, J., Bošković, M., Tasić, G., Vasilijević, B., Marković, D., Marković, T.,& Nikolić, B.. (2022). Cymbopogon martinii essential oil nanoemulsions: Physico-chemical characterization, antibacterial and antibiofilm potential against Enterococcus faecalis. in Industrial Crops and Products, 187, 115478.
https://doi.org/10.1016/j.indcrop.2022.115478

Marinković J, Bošković M, Tasić G, Vasilijević B, Marković D, Marković T, Nikolić B. Cymbopogon martinii essential oil nanoemulsions: Physico-chemical characterization, antibacterial and antibiofilm potential against Enterococcus faecalis. in Industrial Crops and Products. 2022;187:115478.
doi:10.1016/j.indcrop.2022.115478 .

Marinković, Jelena, Bošković, Marko, Tasić, Gvozden, Vasilijević, Bojana, Marković, Dejan, Marković, Tatjana, Nikolić, Biljana, "Cymbopogon martinii essential oil nanoemulsions: Physico-chemical characterization, antibacterial and antibiofilm potential against Enterococcus faecalis" in Industrial Crops and Products, 187 (2022):115478,
https://doi.org/10.1016/j.indcrop.2022.115478 . .

TY  - JOUR
AU  - Marinković, Jelena
AU  - Nikolić, Biljana
AU  - Marković, Tatjana
AU  - Petrović, Božana
AU  - Pašalić, Snežana
AU  - Lal, Mohan
AU  - Marković, Dejan
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10515
AB  - To enhance endodontics, the search for new antibacterials that can improve infected tooth root canal treatment is ongoing. As potent antibacterial and antibiofilm agents, essential oils (EOs) have been suggested as novel endodontic materials. Several studies indicate that EO-based irrigants and medicaments show promising reductive potential against the most important intracanal pathogen, Enterococcus faecalis, and notably contribute to intracanal biofilm eradication. In terms of additional benefits that EO-based endodontic materials can provide, their antioxidant and anti-inflammatory potential are also important, but they have only scarcely been explored in research. Investigations into the benefits of EO-based endodontic materials together with their biocompatibility are needed. The results presented in this review strongly encourage further research on this topic.
T2  - Future Microbiology
T1  - Essential oils as adjuvants in endodontic therapy: myth or reality?
VL  - 17
IS  - 18
SP  - 1487
EP  - 1499
DO  - 10.2217/fmb-2022-0115
ER  - 

@article{
author = "Marinković, Jelena and Nikolić, Biljana and Marković, Tatjana and Petrović, Božana and Pašalić, Snežana and Lal, Mohan and Marković, Dejan",
year = "2022",
abstract = "To enhance endodontics, the search for new antibacterials that can improve infected tooth root canal treatment is ongoing. As potent antibacterial and antibiofilm agents, essential oils (EOs) have been suggested as novel endodontic materials. Several studies indicate that EO-based irrigants and medicaments show promising reductive potential against the most important intracanal pathogen, Enterococcus faecalis, and notably contribute to intracanal biofilm eradication. In terms of additional benefits that EO-based endodontic materials can provide, their antioxidant and anti-inflammatory potential are also important, but they have only scarcely been explored in research. Investigations into the benefits of EO-based endodontic materials together with their biocompatibility are needed. The results presented in this review strongly encourage further research on this topic.",
journal = "Future Microbiology",
title = "Essential oils as adjuvants in endodontic therapy: myth or reality?",
volume = "17",
number = "18",
pages = "1487-1499",
doi = "10.2217/fmb-2022-0115"
}

Marinković, J., Nikolić, B., Marković, T., Petrović, B., Pašalić, S., Lal, M.,& Marković, D.. (2022). Essential oils as adjuvants in endodontic therapy: myth or reality?. in Future Microbiology, 17(18), 1487-1499.
https://doi.org/10.2217/fmb-2022-0115

Marinković J, Nikolić B, Marković T, Petrović B, Pašalić S, Lal M, Marković D. Essential oils as adjuvants in endodontic therapy: myth or reality?. in Future Microbiology. 2022;17(18):1487-1499.
doi:10.2217/fmb-2022-0115 .

Marinković, Jelena, Nikolić, Biljana, Marković, Tatjana, Petrović, Božana, Pašalić, Snežana, Lal, Mohan, Marković, Dejan, "Essential oils as adjuvants in endodontic therapy: myth or reality?" in Future Microbiology, 17, no. 18 (2022):1487-1499,
https://doi.org/10.2217/fmb-2022-0115 . .

TY  - JOUR
AU  - Marinković, Jelena
AU  - Nikolić, Biljana
AU  - Marković, Tatjana
AU  - Radunović, Milena
AU  - Ilić, Jugoslav
AU  - Bošković, Marko
AU  - Ćirić, Ana
AU  - Marković, Dejan
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9906
AB  - Aim: The objective was to formulate and characterize the nanoemulsion based on Cymbopogon citratus oil, intended for use in infected teeth root canal therapy. The investigation of the antioxidant and antibiofilm potential toward Enterococcus faecalis was aimed as well. Materials & methods: Characterization of oil (by GC/MS analysis) and nanoemulsion (by dynamic light scattering instrument), and determination of antibacterial (by microdilution assay), antibiofilm (by crystal violet assay) and antioxidant properties (by 2,2-diphenyl-1-picryl-hydrazyl-hydrate and thiobarbituric acid assay methods) were provided. Antibiofilm efficacy of irrigation procedure including nanoemulsion was screened on extracted teeth (by CFU-counting assay). Results: Notable antibacterial and antibiofilm activity, both against forming and preformed biofilms of oil, was observed. Irrigation involved nanoemulsion showed remarkable antibiofilm potential. Both substances induced some antioxidant activity. Conclusion: Results encourage further research with the aim of application of the nanoemulsion in dental practice.
T2  - Future Microbiology
T1  - Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm
VL  - 16
IS  - 12
SP  - 907
EP  - 918
DO  - 10.2217/fmb-2021-0081
ER  - 

@article{
author = "Marinković, Jelena and Nikolić, Biljana and Marković, Tatjana and Radunović, Milena and Ilić, Jugoslav and Bošković, Marko and Ćirić, Ana and Marković, Dejan",
year = "2021",
abstract = "Aim: The objective was to formulate and characterize the nanoemulsion based on Cymbopogon citratus oil, intended for use in infected teeth root canal therapy. The investigation of the antioxidant and antibiofilm potential toward Enterococcus faecalis was aimed as well. Materials & methods: Characterization of oil (by GC/MS analysis) and nanoemulsion (by dynamic light scattering instrument), and determination of antibacterial (by microdilution assay), antibiofilm (by crystal violet assay) and antioxidant properties (by 2,2-diphenyl-1-picryl-hydrazyl-hydrate and thiobarbituric acid assay methods) were provided. Antibiofilm efficacy of irrigation procedure including nanoemulsion was screened on extracted teeth (by CFU-counting assay). Results: Notable antibacterial and antibiofilm activity, both against forming and preformed biofilms of oil, was observed. Irrigation involved nanoemulsion showed remarkable antibiofilm potential. Both substances induced some antioxidant activity. Conclusion: Results encourage further research with the aim of application of the nanoemulsion in dental practice.",
journal = "Future Microbiology",
title = "Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm",
volume = "16",
number = "12",
pages = "907-918",
doi = "10.2217/fmb-2021-0081"
}

Marinković, J., Nikolić, B., Marković, T., Radunović, M., Ilić, J., Bošković, M., Ćirić, A.,& Marković, D.. (2021). Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm. in Future Microbiology, 16(12), 907-918.
https://doi.org/10.2217/fmb-2021-0081

Marinković J, Nikolić B, Marković T, Radunović M, Ilić J, Bošković M, Ćirić A, Marković D. Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm. in Future Microbiology. 2021;16(12):907-918.
doi:10.2217/fmb-2021-0081 .

Marinković, Jelena, Nikolić, Biljana, Marković, Tatjana, Radunović, Milena, Ilić, Jugoslav, Bošković, Marko, Ćirić, Ana, Marković, Dejan, "Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm" in Future Microbiology, 16, no. 12 (2021):907-918,
https://doi.org/10.2217/fmb-2021-0081 . .

TY  - JOUR
AU  - Marinković, Jelena
AU  - Marković, Tatjana
AU  - Nikolić, Biljana
AU  - Soldatović, Ivan A.
AU  - Ivanov, Marija
AU  - Ćirić, Ana
AU  - Soković, Marina D.
AU  - Marković, Dejan
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9826
AB  - The study aimed to investigate the potency of Leptospermum petersonii, Eucalyptus citriodora, Pelargonium graveolens, and Pelargonium roseum essential oils (EOs) against Streptococcus sanguinis and Streptococcus salivarius dental isolates. To examine the variation in sensitivity among strains of different origin, isolates from symptomatic and asymptomatic root canal infections, as well as the reference, strains S. sanguinis ATCC 10556 and S. salivarius ATCC 9222 were used in the study. EOs were chemically characterised (GC/MS). Their antibacterial and antibiofilm properties were detected by microdilution and CV assay respectively. Statistical analysis was performed by One way ANOVA. Examined bacterial strains, regardless clinical or ATCC, exhibited the highest sensitivity to L. petersonii and E. citriodora EOs (MIC ranged 0.53±0.00 - 5.88±1.27 mg/ml and 0.08±0.03 - 9.37±1.25 mg/ml respectively), while two other EOs were less efficient. Comparison of MIC values showed that sensitivity of the isolates recovered from symptomatic infections was the least, while the susceptibility of asymptomatic isolates and especially of reference strains were higher. EO inhibition of biofilm formation differed between clinical isolates of S. salivarius and their reference strain. Inhibition was higher in the case of S. salivarius ATCC 9222 (84 %-89 %) in comparison to that of clinical isolates (24 %-75 %). Regardless of the strains tested, E. citriodora exhibited the highest antibiofilm potential. To conclude: L. petersonii induced notable antibacterial potential, while E. citriodora EO proved promising antibacterial and antibiofilm activity. Due to the variable sensitivity of the isolates to EOs, it is suggested to screen activity on a large number of strains, especially from symptomatic infections.
T2  - Journal of Essential Oil Bearing Plants
T1  - Antibacterial and Antibiofilm Potential of Leptospermum petersonii F.M.Bailey, Eucalyptus citriodora Hook., Pelargonium graveolens L’Hér. and Pelargonium roseum (Andrews) DC. Essential Oils Against Selected Dental Isolates
VL  - 24
IS  - 2
SP  - 304
EP  - 316
DO  - 10.1080/0972060X.2021.1899059
ER  - 

@article{
author = "Marinković, Jelena and Marković, Tatjana and Nikolić, Biljana and Soldatović, Ivan A. and Ivanov, Marija and Ćirić, Ana and Soković, Marina D. and Marković, Dejan",
year = "2021",
abstract = "The study aimed to investigate the potency of Leptospermum petersonii, Eucalyptus citriodora, Pelargonium graveolens, and Pelargonium roseum essential oils (EOs) against Streptococcus sanguinis and Streptococcus salivarius dental isolates. To examine the variation in sensitivity among strains of different origin, isolates from symptomatic and asymptomatic root canal infections, as well as the reference, strains S. sanguinis ATCC 10556 and S. salivarius ATCC 9222 were used in the study. EOs were chemically characterised (GC/MS). Their antibacterial and antibiofilm properties were detected by microdilution and CV assay respectively. Statistical analysis was performed by One way ANOVA. Examined bacterial strains, regardless clinical or ATCC, exhibited the highest sensitivity to L. petersonii and E. citriodora EOs (MIC ranged 0.53±0.00 - 5.88±1.27 mg/ml and 0.08±0.03 - 9.37±1.25 mg/ml respectively), while two other EOs were less efficient. Comparison of MIC values showed that sensitivity of the isolates recovered from symptomatic infections was the least, while the susceptibility of asymptomatic isolates and especially of reference strains were higher. EO inhibition of biofilm formation differed between clinical isolates of S. salivarius and their reference strain. Inhibition was higher in the case of S. salivarius ATCC 9222 (84 %-89 %) in comparison to that of clinical isolates (24 %-75 %). Regardless of the strains tested, E. citriodora exhibited the highest antibiofilm potential. To conclude: L. petersonii induced notable antibacterial potential, while E. citriodora EO proved promising antibacterial and antibiofilm activity. Due to the variable sensitivity of the isolates to EOs, it is suggested to screen activity on a large number of strains, especially from symptomatic infections.",
journal = "Journal of Essential Oil Bearing Plants",
title = "Antibacterial and Antibiofilm Potential of Leptospermum petersonii F.M.Bailey, Eucalyptus citriodora Hook., Pelargonium graveolens L’Hér. and Pelargonium roseum (Andrews) DC. Essential Oils Against Selected Dental Isolates",
volume = "24",
number = "2",
pages = "304-316",
doi = "10.1080/0972060X.2021.1899059"
}

Marinković, J., Marković, T., Nikolić, B., Soldatović, I. A., Ivanov, M., Ćirić, A., Soković, M. D.,& Marković, D.. (2021). Antibacterial and Antibiofilm Potential of Leptospermum petersonii F.M.Bailey, Eucalyptus citriodora Hook., Pelargonium graveolens L’Hér. and Pelargonium roseum (Andrews) DC. Essential Oils Against Selected Dental Isolates. in Journal of Essential Oil Bearing Plants, 24(2), 304-316.
https://doi.org/10.1080/0972060X.2021.1899059

Marinković J, Marković T, Nikolić B, Soldatović IA, Ivanov M, Ćirić A, Soković MD, Marković D. Antibacterial and Antibiofilm Potential of Leptospermum petersonii F.M.Bailey, Eucalyptus citriodora Hook., Pelargonium graveolens L’Hér. and Pelargonium roseum (Andrews) DC. Essential Oils Against Selected Dental Isolates. in Journal of Essential Oil Bearing Plants. 2021;24(2):304-316.
doi:10.1080/0972060X.2021.1899059 .

Marinković, Jelena, Marković, Tatjana, Nikolić, Biljana, Soldatović, Ivan A., Ivanov, Marija, Ćirić, Ana, Soković, Marina D., Marković, Dejan, "Antibacterial and Antibiofilm Potential of Leptospermum petersonii F.M.Bailey, Eucalyptus citriodora Hook., Pelargonium graveolens L’Hér. and Pelargonium roseum (Andrews) DC. Essential Oils Against Selected Dental Isolates" in Journal of Essential Oil Bearing Plants, 24, no. 2 (2021):304-316,
https://doi.org/10.1080/0972060X.2021.1899059 . .

TY  - JOUR
AU  - Marinković, Jelena
AU  - Mitić Ćulafić, Dragana
AU  - Nikolić, Biljana
AU  - Đukanović, Stefana
AU  - Marković, Tatjana
AU  - Tasić, Gvozden
AU  - Ćirić, Ana
AU  - Marković, Dejan
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9081
AB  - OBJECTIVE The objective was to determine the antibiofilm efficacy of Cymbopogon martinii and Thymus zygis essential oils and to estimate the disruption potential of oil-based endodontic irrigants on the multispecies biofilm formed in the root canals of extracted teeth. DESIGN The essential oils were characterized (GC-MS), while their antibacterial and cytotoxic properties were detected by microdilution and MTT assays. Particles sizes and polydispersity indices were determined for the irrigants. The isolates from root canals of pediatric patients were identified (MALDI-TOF). The multispecies biofilms were formed from Streptococcus mitis, Streptococcus sanguinis and Enterococcus faecalis. Antibiofilm properties of the essential oils and irrigants were determined by crystal violet and plate counting assay. Statistical analysis was performed by one-way ANOVA. RESULTS The screening of biofilm biomass revealed a disruption potential of C. martinii essential oil. The plate counting assay showed the efficacy of both oils in diminishing cell viability: high in biofilms (reduction of log10CFU was 2.75-2.87) and moderate in the planktons formed above. The essential oil-based irrigants showed the same antibiofilm activity as in the control (1.5 % sodium hypochlorite) for C. martini and almost 2-fold higher for T. zygis. Successive irrigations with 1.5 % sodium hypochlorite, saline and an oil-based irrigant was more efficient for C. martini than for the control (reductions of log10CFU was 1.69 and 1.14, respectively). CONCLUSIONS Notable activities achieved by essential oils and oil-based irrigants, particularly regarding C. martini, against the biofilm consisting of S. mitis, S. sanguinis, and E. faecalis, are expected to encourage further research in endodontics.
T2  - Archives of Oral Biology
T1  - Antimicrobial potential of irrigants based on essential oils of Cymbopogon martinii and Thymus zygis towards in vitro multispecies biofilm cultured in ex vivo root canals.
VL  - 117
SP  - 104842
DO  - 10.1016/j.archoralbio.2020.104842
ER  - 

@article{
author = "Marinković, Jelena and Mitić Ćulafić, Dragana and Nikolić, Biljana and Đukanović, Stefana and Marković, Tatjana and Tasić, Gvozden and Ćirić, Ana and Marković, Dejan",
year = "2020",
abstract = "OBJECTIVE The objective was to determine the antibiofilm efficacy of Cymbopogon martinii and Thymus zygis essential oils and to estimate the disruption potential of oil-based endodontic irrigants on the multispecies biofilm formed in the root canals of extracted teeth. DESIGN The essential oils were characterized (GC-MS), while their antibacterial and cytotoxic properties were detected by microdilution and MTT assays. Particles sizes and polydispersity indices were determined for the irrigants. The isolates from root canals of pediatric patients were identified (MALDI-TOF). The multispecies biofilms were formed from Streptococcus mitis, Streptococcus sanguinis and Enterococcus faecalis. Antibiofilm properties of the essential oils and irrigants were determined by crystal violet and plate counting assay. Statistical analysis was performed by one-way ANOVA. RESULTS The screening of biofilm biomass revealed a disruption potential of C. martinii essential oil. The plate counting assay showed the efficacy of both oils in diminishing cell viability: high in biofilms (reduction of log10CFU was 2.75-2.87) and moderate in the planktons formed above. The essential oil-based irrigants showed the same antibiofilm activity as in the control (1.5 % sodium hypochlorite) for C. martini and almost 2-fold higher for T. zygis. Successive irrigations with 1.5 % sodium hypochlorite, saline and an oil-based irrigant was more efficient for C. martini than for the control (reductions of log10CFU was 1.69 and 1.14, respectively). CONCLUSIONS Notable activities achieved by essential oils and oil-based irrigants, particularly regarding C. martini, against the biofilm consisting of S. mitis, S. sanguinis, and E. faecalis, are expected to encourage further research in endodontics.",
journal = "Archives of Oral Biology",
title = "Antimicrobial potential of irrigants based on essential oils of Cymbopogon martinii and Thymus zygis towards in vitro multispecies biofilm cultured in ex vivo root canals.",
volume = "117",
pages = "104842",
doi = "10.1016/j.archoralbio.2020.104842"
}

Marinković, J., Mitić Ćulafić, D., Nikolić, B., Đukanović, S., Marković, T., Tasić, G., Ćirić, A.,& Marković, D.. (2020). Antimicrobial potential of irrigants based on essential oils of Cymbopogon martinii and Thymus zygis towards in vitro multispecies biofilm cultured in ex vivo root canals.. in Archives of Oral Biology, 117, 104842.
https://doi.org/10.1016/j.archoralbio.2020.104842

Marinković J, Mitić Ćulafić D, Nikolić B, Đukanović S, Marković T, Tasić G, Ćirić A, Marković D. Antimicrobial potential of irrigants based on essential oils of Cymbopogon martinii and Thymus zygis towards in vitro multispecies biofilm cultured in ex vivo root canals.. in Archives of Oral Biology. 2020;117:104842.
doi:10.1016/j.archoralbio.2020.104842 .

Marinković, Jelena, Mitić Ćulafić, Dragana, Nikolić, Biljana, Đukanović, Stefana, Marković, Tatjana, Tasić, Gvozden, Ćirić, Ana, Marković, Dejan, "Antimicrobial potential of irrigants based on essential oils of Cymbopogon martinii and Thymus zygis towards in vitro multispecies biofilm cultured in ex vivo root canals." in Archives of Oral Biology, 117 (2020):104842,
https://doi.org/10.1016/j.archoralbio.2020.104842 . .

TY  - JOUR
AU  - Marinković, Jelena
AU  - Marković, Tatjana
AU  - Brkić, Snežana
AU  - Radunović, Milena
AU  - Soldatović, Ivan
AU  - Ćirić, Ana
AU  - Marković, Dejan
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10808
AB  - Background/Aim: Understanding the composition of bacteria in infected root canals is important for ameliorating the treatment strategies that lead to the elimination of pathogens and infection control, but also prevent reinfection. Aim of this study was to investigate microbial composition of primary infected root canals with apical periodontitis of young permanent teeth, originating form school children in Serbia, and its association with clinical symptoms. Material and Methods: To determine the bacterial composition of infected root canals in children, 35 endodontic samples were obtained. The identification of cultured bacteria was performed by MALDI-TOF MS analysis. The presence or absence of clinical symptoms were recorded. Results: Facultative anaerobes were 2,2 times more frequent than obligate anaerobes. The most common facultative anaerobes belonged to following genera, Streptococcus (58 isolates), Actinomyces (10) and Enterococcus (8), while predominant obligate anaerobes, belonged to genera Veillonella (15), Prevotella (9) and Fusobacterium (8). The most common clinical isolates recovered from infected root canals with symptomatic apical periodontitis were Veillonella parvula (10) and Fusobacterium nucleatum (7), while from the asymptomatic ones, they were Streptococcus mitis/Streptococcus oralis (5). Prevalence of Parvimonas micra, Prevotella buccae and Streptococcus constellatus within the root canals might be associated to clinical symptoms. Conclusions: Species of genera Streptococcus and Veillonella were the most common isolates from primary infected root canals with apical periodontitis in Serbian school children. Facultative anaerobes were predominant over obligate anaerobes. The prevalence of obligate anaerobes was much higher in symptomatic compared to asymptomatic root canal infections. No specific bacterial strain might be associated to a single examined clinical symptom (pain, tenderness to percussion or swelling), but majority of the strains are associated to all of the examined three symptoms.
T2  - Balkan Journal of Dental Medicine
T1  - Microbiological analysis of primary infected root canals with symptomatic and asymptomatic apical periodontitis of young permanent teeth
VL  - 24
IS  - 3
SP  - 170
EP  - 177
DO  - 10.2478/bjdm-2020-0027
ER  - 

@article{
author = "Marinković, Jelena and Marković, Tatjana and Brkić, Snežana and Radunović, Milena and Soldatović, Ivan and Ćirić, Ana and Marković, Dejan",
year = "2020",
abstract = "Background/Aim: Understanding the composition of bacteria in infected root canals is important for ameliorating the treatment strategies that lead to the elimination of pathogens and infection control, but also prevent reinfection. Aim of this study was to investigate microbial composition of primary infected root canals with apical periodontitis of young permanent teeth, originating form school children in Serbia, and its association with clinical symptoms. Material and Methods: To determine the bacterial composition of infected root canals in children, 35 endodontic samples were obtained. The identification of cultured bacteria was performed by MALDI-TOF MS analysis. The presence or absence of clinical symptoms were recorded. Results: Facultative anaerobes were 2,2 times more frequent than obligate anaerobes. The most common facultative anaerobes belonged to following genera, Streptococcus (58 isolates), Actinomyces (10) and Enterococcus (8), while predominant obligate anaerobes, belonged to genera Veillonella (15), Prevotella (9) and Fusobacterium (8). The most common clinical isolates recovered from infected root canals with symptomatic apical periodontitis were Veillonella parvula (10) and Fusobacterium nucleatum (7), while from the asymptomatic ones, they were Streptococcus mitis/Streptococcus oralis (5). Prevalence of Parvimonas micra, Prevotella buccae and Streptococcus constellatus within the root canals might be associated to clinical symptoms. Conclusions: Species of genera Streptococcus and Veillonella were the most common isolates from primary infected root canals with apical periodontitis in Serbian school children. Facultative anaerobes were predominant over obligate anaerobes. The prevalence of obligate anaerobes was much higher in symptomatic compared to asymptomatic root canal infections. No specific bacterial strain might be associated to a single examined clinical symptom (pain, tenderness to percussion or swelling), but majority of the strains are associated to all of the examined three symptoms.",
journal = "Balkan Journal of Dental Medicine",
title = "Microbiological analysis of primary infected root canals with symptomatic and asymptomatic apical periodontitis of young permanent teeth",
volume = "24",
number = "3",
pages = "170-177",
doi = "10.2478/bjdm-2020-0027"
}

Marinković, J., Marković, T., Brkić, S., Radunović, M., Soldatović, I., Ćirić, A.,& Marković, D.. (2020). Microbiological analysis of primary infected root canals with symptomatic and asymptomatic apical periodontitis of young permanent teeth. in Balkan Journal of Dental Medicine, 24(3), 170-177.
https://doi.org/10.2478/bjdm-2020-0027

Marinković J, Marković T, Brkić S, Radunović M, Soldatović I, Ćirić A, Marković D. Microbiological analysis of primary infected root canals with symptomatic and asymptomatic apical periodontitis of young permanent teeth. in Balkan Journal of Dental Medicine. 2020;24(3):170-177.
doi:10.2478/bjdm-2020-0027 .

Marinković, Jelena, Marković, Tatjana, Brkić, Snežana, Radunović, Milena, Soldatović, Ivan, Ćirić, Ana, Marković, Dejan, "Microbiological analysis of primary infected root canals with symptomatic and asymptomatic apical periodontitis of young permanent teeth" in Balkan Journal of Dental Medicine, 24, no. 3 (2020):170-177,
https://doi.org/10.2478/bjdm-2020-0027 . .

TY  - JOUR
AU  - Bogojević, Jelena
AU  - Nikolić, Miloš
AU  - Marković, Tatjana
AU  - Ćirić, Ana
AU  - Marković, Dejan
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11675
AB  - Twenty one essential oils (EOs) documented their significant antimicrobial effect with regard to our pre-set criterion of the Minimal Inhibitory Concentration (MIC ≤ 200 μL / mL) of EOs towards Enterococcus faecalis (ATCC 29212 and or clinical isolates); the best effect MIC 0.4 μL / mL (approx. 0.26 μg / mL) achieved Satureja horvatii L. EO, while the EOs with the lowest antimicrobial efficacy were Rosmarinus officinalis L. and Achilea milefolium L., both with MIC s160.0 μg / mL. Analysis of the MIC values within the groups revealed that ATCC strain of E. faecalis is generally lower, ranging from 0.26 to 156 μg / mL, in comparison to those for clinical isolateswhich ranged from 10 to 160 μg / mL. Twelve 12 components that are common in EOs whith MIC s ≤ 200 μg / mL in testings towards both, the clinical and referent strains are given in descending order according to number of oils they are present in: trans-β-caryophyllene (13) > myrcene (8) > α-pinene (8) > linalool (7) > p-cymene (7) > borneol (7) > geraniol (6) > camphene (6) > limonene (5) > 1,8- cineol (5) > γ -terpinene (5) > α- terpinene (4). Comparison of EO constituents reviled that only, geraniol and 1,8-cineol, contributed with ≥ 10 % to more than one EO (MIC 0.3-200 μg / mL) efficient against both E. faecalis strains. Thirteen components in 11 EOs with MIC ≤ 200 μg / mL towards ATCC 29212 were representative based on their contents in EOs: eugenol 82.9 % > thymol 63.7 % > hexadecanoic acid 47.8 % > menthol 46.6 % > cis-b-ocimene 44.2 % > geranial 42.1 % > trans-β-caryophyllene 40.8 % > citronellal 36.7 % > α-pinene 31.2 % > neral 30.5 % > α-eudesmol 22.4 % > citronellol 13.1 % > menthone 11.3 %. Following seven components, representative in 10 EOs with MIC ≤ 200 μg / mL towards clinical isolates, are presented in order of their contribution to EOs: phenylethyl alcohol 57.7 % > geranial 32.9 % > neral 22.2 % > p- cymene 20 % > carvacrol 14 % > α-pinene 11.5 % > linalool 11.4 %. Out of 21 highly efficient EOs selected in this study, six EOs proved to be the most efficient (MIC ≤ 30 μg / mL ); three oils in control of E.faecalis ATCC strain (Satureja horvatii, Mentha pulegium and Rosmarinus officinalis) and other three in control of E. faecalis clinical isolates (Leptospermum petersonii, Thymus algeriensis, Thymus serpyllum). Thymol is a major component in three out of the six aforementioned most efficient EOs. The aim of our study was to investigate differences in efficacy of selected EOs that proved to possess great antimicrobial activity, towards the referent strain ATCC 29212 and clinical isolates of E. faecalis on, and to estimate which of their constituents might contribute to desired activity, as 'markers compunds'.
T2  - Lekovite sirovine
T1  - Analysis of chemical composition of the most efficient essential oils towards Enterococcus faecalis referent strain ATCC 29212 and clinical isolates
IS  - 36
SP  - 3
EP  - 25
DO  - 10.5937/leksir1636003B
ER  - 

@article{
author = "Bogojević, Jelena and Nikolić, Miloš and Marković, Tatjana and Ćirić, Ana and Marković, Dejan",
year = "2016",
abstract = "Twenty one essential oils (EOs) documented their significant antimicrobial effect with regard to our pre-set criterion of the Minimal Inhibitory Concentration (MIC ≤ 200 μL / mL) of EOs towards Enterococcus faecalis (ATCC 29212 and or clinical isolates); the best effect MIC 0.4 μL / mL (approx. 0.26 μg / mL) achieved Satureja horvatii L. EO, while the EOs with the lowest antimicrobial efficacy were Rosmarinus officinalis L. and Achilea milefolium L., both with MIC s160.0 μg / mL. Analysis of the MIC values within the groups revealed that ATCC strain of E. faecalis is generally lower, ranging from 0.26 to 156 μg / mL, in comparison to those for clinical isolateswhich ranged from 10 to 160 μg / mL. Twelve 12 components that are common in EOs whith MIC s ≤ 200 μg / mL in testings towards both, the clinical and referent strains are given in descending order according to number of oils they are present in: trans-β-caryophyllene (13) > myrcene (8) > α-pinene (8) > linalool (7) > p-cymene (7) > borneol (7) > geraniol (6) > camphene (6) > limonene (5) > 1,8- cineol (5) > γ -terpinene (5) > α- terpinene (4). Comparison of EO constituents reviled that only, geraniol and 1,8-cineol, contributed with ≥ 10 % to more than one EO (MIC 0.3-200 μg / mL) efficient against both E. faecalis strains. Thirteen components in 11 EOs with MIC ≤ 200 μg / mL towards ATCC 29212 were representative based on their contents in EOs: eugenol 82.9 % > thymol 63.7 % > hexadecanoic acid 47.8 % > menthol 46.6 % > cis-b-ocimene 44.2 % > geranial 42.1 % > trans-β-caryophyllene 40.8 % > citronellal 36.7 % > α-pinene 31.2 % > neral 30.5 % > α-eudesmol 22.4 % > citronellol 13.1 % > menthone 11.3 %. Following seven components, representative in 10 EOs with MIC ≤ 200 μg / mL towards clinical isolates, are presented in order of their contribution to EOs: phenylethyl alcohol 57.7 % > geranial 32.9 % > neral 22.2 % > p- cymene 20 % > carvacrol 14 % > α-pinene 11.5 % > linalool 11.4 %. Out of 21 highly efficient EOs selected in this study, six EOs proved to be the most efficient (MIC ≤ 30 μg / mL ); three oils in control of E.faecalis ATCC strain (Satureja horvatii, Mentha pulegium and Rosmarinus officinalis) and other three in control of E. faecalis clinical isolates (Leptospermum petersonii, Thymus algeriensis, Thymus serpyllum). Thymol is a major component in three out of the six aforementioned most efficient EOs. The aim of our study was to investigate differences in efficacy of selected EOs that proved to possess great antimicrobial activity, towards the referent strain ATCC 29212 and clinical isolates of E. faecalis on, and to estimate which of their constituents might contribute to desired activity, as 'markers compunds'.",
journal = "Lekovite sirovine",
title = "Analysis of chemical composition of the most efficient essential oils towards Enterococcus faecalis referent strain ATCC 29212 and clinical isolates",
number = "36",
pages = "3-25",
doi = "10.5937/leksir1636003B"
}

Bogojević, J., Nikolić, M., Marković, T., Ćirić, A.,& Marković, D.. (2016). Analysis of chemical composition of the most efficient essential oils towards Enterococcus faecalis referent strain ATCC 29212 and clinical isolates. in Lekovite sirovine(36), 3-25.
https://doi.org/10.5937/leksir1636003B

Bogojević J, Nikolić M, Marković T, Ćirić A, Marković D. Analysis of chemical composition of the most efficient essential oils towards Enterococcus faecalis referent strain ATCC 29212 and clinical isolates. in Lekovite sirovine. 2016;(36):3-25.
doi:10.5937/leksir1636003B .

Bogojević, Jelena, Nikolić, Miloš, Marković, Tatjana, Ćirić, Ana, Marković, Dejan, "Analysis of chemical composition of the most efficient essential oils towards Enterococcus faecalis referent strain ATCC 29212 and clinical isolates" in Lekovite sirovine, no. 36 (2016):3-25,
https://doi.org/10.5937/leksir1636003B . .