TY  - JOUR
AU  - Čairović, Aleksandra
AU  - Maksimović, Vesna
AU  - Radović, Katarina
AU  - Đurišić, Slobodan
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1410
AB  - Introduction Increases in market prices of gold over the last 20 years have led to expansion of basic dental alloys, which, primarily due to their good mechanical properties and acceptable prices, have found their place in everyday dental practice. However, within the procedure of making dental prosthetic restorations, the alloys are melted and cast, which leads to changes in their physical, mechanical and biological properties. Objective The objective of the study was to test biocompatibility of a Ni-Cr dental alloy (WIRON 99) depending on the number of melting and casting processes. Methods The working method included the testing of cytotoxicity of the alloy obtained by casting after one, after four, and after eight successive processes of melting. Cytotoxicity of samples was tested by means of a 24-hour and a three-day cytotoxicity test, done on L929 fibroblasts. Results A repeatedly melted and cast alloy shows a reduced biocompatibility and causes specific responses of the tissues in the surrounding area. Since the cytotoxic effect is more significant in the extended contact with the culture cells, a three-day cytotoxicity test showed discrete changes which were the indicator of cell growth inhibition in the cell culture. Conclusion The obtained results confirm the working hypothesis that repeated alloy melting and casting will decrease biocompatibility of dental alloys and will lead to specific responses of the tissue in the surrounding area.
T2  - Srpski arhiv za celokupno lekarstvo
T1  - The effect of recasting on biological properties of Ni-Cr dental alloy
VL  - 144
IS  - 11-12
SP  - 574
EP  - 579
DO  - 10.2298/SARH1612574C
ER  - 

@article{
author = "Čairović, Aleksandra and Maksimović, Vesna and Radović, Katarina and Đurišić, Slobodan",
year = "2016",
abstract = "Introduction Increases in market prices of gold over the last 20 years have led to expansion of basic dental alloys, which, primarily due to their good mechanical properties and acceptable prices, have found their place in everyday dental practice. However, within the procedure of making dental prosthetic restorations, the alloys are melted and cast, which leads to changes in their physical, mechanical and biological properties. Objective The objective of the study was to test biocompatibility of a Ni-Cr dental alloy (WIRON 99) depending on the number of melting and casting processes. Methods The working method included the testing of cytotoxicity of the alloy obtained by casting after one, after four, and after eight successive processes of melting. Cytotoxicity of samples was tested by means of a 24-hour and a three-day cytotoxicity test, done on L929 fibroblasts. Results A repeatedly melted and cast alloy shows a reduced biocompatibility and causes specific responses of the tissues in the surrounding area. Since the cytotoxic effect is more significant in the extended contact with the culture cells, a three-day cytotoxicity test showed discrete changes which were the indicator of cell growth inhibition in the cell culture. Conclusion The obtained results confirm the working hypothesis that repeated alloy melting and casting will decrease biocompatibility of dental alloys and will lead to specific responses of the tissue in the surrounding area.",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "The effect of recasting on biological properties of Ni-Cr dental alloy",
volume = "144",
number = "11-12",
pages = "574-579",
doi = "10.2298/SARH1612574C"
}

Čairović, A., Maksimović, V., Radović, K.,& Đurišić, S.. (2016). The effect of recasting on biological properties of Ni-Cr dental alloy. in Srpski arhiv za celokupno lekarstvo, 144(11-12), 574-579.
https://doi.org/10.2298/SARH1612574C

Čairović A, Maksimović V, Radović K, Đurišić S. The effect of recasting on biological properties of Ni-Cr dental alloy. in Srpski arhiv za celokupno lekarstvo. 2016;144(11-12):574-579.
doi:10.2298/SARH1612574C .

Čairović, Aleksandra, Maksimović, Vesna, Radović, Katarina, Đurišić, Slobodan, "The effect of recasting on biological properties of Ni-Cr dental alloy" in Srpski arhiv za celokupno lekarstvo, 144, no. 11-12 (2016):574-579,
https://doi.org/10.2298/SARH1612574C . .

TY  - JOUR
AU  - Čairović, Aleksandra
AU  - Djordjevic, Ivan
AU  - Bulatovic, Mirna
AU  - Mojic, Marija
AU  - Momčilović, Miljana
AU  - Stošić-Grujičić, Stanislava
AU  - Maksimović, Vesna
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Stamenkovic, Dragoslav
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5620
AB  - The aim of this study was to evaluate the effect of recasting of commercially available Ni-Cr (Wiron 99) and Co-Cr (Dentalit C) dental alloys on physiology of microenvironmental cells. The viability of fibrosarcoma (L929) cells, human embrional fibroblasts (MRC-5) and isolated peripheral blood mononuclear cells (PBMC) was measured by MTT and acidic phosphatase tests. Presence of dying cells was estimated by Annexin/PI staining while the production of intracellular nitric oxide (NO), reactive oxygen (ROS) and nitrogen (RNS) species was determined by DAF-FM diacetate and DHR staining. Recasting of Ni-Cr alloy intensified its cytotoxicity manifested through enhanced free radicals production, induction of cell death and permamently diminished cell proliferation. On the other hand, after initial toxic effect cells adapted to the presence of Co-Cr alloys. Independently of recasting, Co-Cr alloys are more compatible with microenvironment then Ni-Cr alloy. Oppositely, recasting of Ni-Cr alloy promoted its toxicity.
T2  - Digest Journal of Nanomaterials and Biostructures
T1  - In Vitro Assessment of Ni-Cr and Co-Cr Dental Alloys Upon Recasting: Cellular Compatibility
VL  - 8
IS  - 2
SP  - 877
EP  - U461
UR  - https://hdl.handle.net/21.15107/rcub_vinar_5620
ER  - 

@article{
author = "Čairović, Aleksandra and Djordjevic, Ivan and Bulatovic, Mirna and Mojic, Marija and Momčilović, Miljana and Stošić-Grujičić, Stanislava and Maksimović, Vesna and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Stamenkovic, Dragoslav",
year = "2013",
abstract = "The aim of this study was to evaluate the effect of recasting of commercially available Ni-Cr (Wiron 99) and Co-Cr (Dentalit C) dental alloys on physiology of microenvironmental cells. The viability of fibrosarcoma (L929) cells, human embrional fibroblasts (MRC-5) and isolated peripheral blood mononuclear cells (PBMC) was measured by MTT and acidic phosphatase tests. Presence of dying cells was estimated by Annexin/PI staining while the production of intracellular nitric oxide (NO), reactive oxygen (ROS) and nitrogen (RNS) species was determined by DAF-FM diacetate and DHR staining. Recasting of Ni-Cr alloy intensified its cytotoxicity manifested through enhanced free radicals production, induction of cell death and permamently diminished cell proliferation. On the other hand, after initial toxic effect cells adapted to the presence of Co-Cr alloys. Independently of recasting, Co-Cr alloys are more compatible with microenvironment then Ni-Cr alloy. Oppositely, recasting of Ni-Cr alloy promoted its toxicity.",
journal = "Digest Journal of Nanomaterials and Biostructures",
title = "In Vitro Assessment of Ni-Cr and Co-Cr Dental Alloys Upon Recasting: Cellular Compatibility",
volume = "8",
number = "2",
pages = "877-U461",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5620"
}

Čairović, A., Djordjevic, I., Bulatovic, M., Mojic, M., Momčilović, M., Stošić-Grujičić, S., Maksimović, V., Maksimović-Ivanić, D., Mijatović, S.,& Stamenkovic, D.. (2013). In Vitro Assessment of Ni-Cr and Co-Cr Dental Alloys Upon Recasting: Cellular Compatibility. in Digest Journal of Nanomaterials and Biostructures, 8(2), 877-U461.
https://hdl.handle.net/21.15107/rcub_vinar_5620

Čairović A, Djordjevic I, Bulatovic M, Mojic M, Momčilović M, Stošić-Grujičić S, Maksimović V, Maksimović-Ivanić D, Mijatović S, Stamenkovic D. In Vitro Assessment of Ni-Cr and Co-Cr Dental Alloys Upon Recasting: Cellular Compatibility. in Digest Journal of Nanomaterials and Biostructures. 2013;8(2):877-U461.
https://hdl.handle.net/21.15107/rcub_vinar_5620 .

Čairović, Aleksandra, Djordjevic, Ivan, Bulatovic, Mirna, Mojic, Marija, Momčilović, Miljana, Stošić-Grujičić, Stanislava, Maksimović, Vesna, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Stamenkovic, Dragoslav, "In Vitro Assessment of Ni-Cr and Co-Cr Dental Alloys Upon Recasting: Cellular Compatibility" in Digest Journal of Nanomaterials and Biostructures, 8, no. 2 (2013):877-U461,
https://hdl.handle.net/21.15107/rcub_vinar_5620 .