TY  - JOUR
AU  - Vodnik, Vesna
AU  - Mojić, Marija
AU  - Stamenović, Una
AU  - Otoničar, Mojca
AU  - Ajdžanović, Vladimir
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Marković, Mirjana
AU  - Barudžija, Tanja
AU  - Filipović, Branko
AU  - Milošević, Verica
AU  - Šošić-Jurjević, Branka
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9749
AB  - Soy isoflavone genistein (Gen) exerts beneficial effects against prostate cancer cells in vitro and in vivo. However, its use as a chemoprevention/therapeutic agent is largely limited due to its low bioavailability. In this study we synthesized two variants of a new delivery system, genistein–gold nanoparticles conjugates Gen@AuNPs1 and Gen@AuNPs2, by an environmentally friendly method, using a dual role of Gen to reduce Au3+ and stabilize the formed AuNPs, with no additional component. The formation of Gen@AuNPs was confirmed via UV–Vis spectroscopy, FTIR, and Raman spectra measurements. The spherical shape and uniform size of Gen@AuNPs1 and Gen@AuNPs2 (10 ± 2 and 23 ± 3 nm, respectively), were determined by transmission electron microscopy. The nano-conjugates also varied in hydrodynamic diameter (65.0 ± 1.7 and 153.0 ± 2.2 nm) but had similar negative zeta potential (−35.0 ± 2.5 and −37.0 ± 1.6 mV), as measured by dynamic light scattering. The Gen loading was estimated to be 46 and 48%, for Gen@AuNPs1 and Gen@AuNPs2, respectively. The antiproliferative activities of GenAuNPs were confirmed by MTT test in vitro on three malignant prostate carcinoma cell lines (PC3, DU 145, and LNCaP), while selectivity toward malignant phenotype was confirmed using non-cancerous MRC-5 cells. Flow cytometric analysis showed that the inhibition on cell proliferation of more potent Gen@AuNPs1 nano-conjugate is comparable with the effects of free Gen. In conclusion, the obtained results, including physicochemical characterization of newly synthesized AuNPs loaded with Gen, cytotoxicity, and IC50 assessments, indicate their stability and bioactivity as an antioxidant and anti-prostate cancer agent, with low toxicity against human primary cells. © 2021 Elsevier B.V.
T2  - Materials Science and Engineering: C
T1  - Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines
VL  - 124
SP  - 112078
DO  - 10.1016/j.msec.2021.112078
ER  - 

@article{
author = "Vodnik, Vesna and Mojić, Marija and Stamenović, Una and Otoničar, Mojca and Ajdžanović, Vladimir and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Marković, Mirjana and Barudžija, Tanja and Filipović, Branko and Milošević, Verica and Šošić-Jurjević, Branka",
year = "2021",
abstract = "Soy isoflavone genistein (Gen) exerts beneficial effects against prostate cancer cells in vitro and in vivo. However, its use as a chemoprevention/therapeutic agent is largely limited due to its low bioavailability. In this study we synthesized two variants of a new delivery system, genistein–gold nanoparticles conjugates Gen@AuNPs1 and Gen@AuNPs2, by an environmentally friendly method, using a dual role of Gen to reduce Au3+ and stabilize the formed AuNPs, with no additional component. The formation of Gen@AuNPs was confirmed via UV–Vis spectroscopy, FTIR, and Raman spectra measurements. The spherical shape and uniform size of Gen@AuNPs1 and Gen@AuNPs2 (10 ± 2 and 23 ± 3 nm, respectively), were determined by transmission electron microscopy. The nano-conjugates also varied in hydrodynamic diameter (65.0 ± 1.7 and 153.0 ± 2.2 nm) but had similar negative zeta potential (−35.0 ± 2.5 and −37.0 ± 1.6 mV), as measured by dynamic light scattering. The Gen loading was estimated to be 46 and 48%, for Gen@AuNPs1 and Gen@AuNPs2, respectively. The antiproliferative activities of GenAuNPs were confirmed by MTT test in vitro on three malignant prostate carcinoma cell lines (PC3, DU 145, and LNCaP), while selectivity toward malignant phenotype was confirmed using non-cancerous MRC-5 cells. Flow cytometric analysis showed that the inhibition on cell proliferation of more potent Gen@AuNPs1 nano-conjugate is comparable with the effects of free Gen. In conclusion, the obtained results, including physicochemical characterization of newly synthesized AuNPs loaded with Gen, cytotoxicity, and IC50 assessments, indicate their stability and bioactivity as an antioxidant and anti-prostate cancer agent, with low toxicity against human primary cells. © 2021 Elsevier B.V.",
journal = "Materials Science and Engineering: C",
title = "Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines",
volume = "124",
pages = "112078",
doi = "10.1016/j.msec.2021.112078"
}

Vodnik, V., Mojić, M., Stamenović, U., Otoničar, M., Ajdžanović, V., Maksimović-Ivanić, D., Mijatović, S., Marković, M., Barudžija, T., Filipović, B., Milošević, V.,& Šošić-Jurjević, B.. (2021). Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines. in Materials Science and Engineering: C, 124, 112078.
https://doi.org/10.1016/j.msec.2021.112078

Vodnik V, Mojić M, Stamenović U, Otoničar M, Ajdžanović V, Maksimović-Ivanić D, Mijatović S, Marković M, Barudžija T, Filipović B, Milošević V, Šošić-Jurjević B. Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines. in Materials Science and Engineering: C. 2021;124:112078.
doi:10.1016/j.msec.2021.112078 .

Vodnik, Vesna, Mojić, Marija, Stamenović, Una, Otoničar, Mojca, Ajdžanović, Vladimir, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Marković, Mirjana, Barudžija, Tanja, Filipović, Branko, Milošević, Verica, Šošić-Jurjević, Branka, "Development of genistein-loaded gold nanoparticles and their antitumor potential against prostate cancer cell lines" in Materials Science and Engineering: C, 124 (2021):112078,
https://doi.org/10.1016/j.msec.2021.112078 . .

TY  - JOUR
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka
AU  - Ajdžanovic, Vladimir
AU  - Živanović, Jasmina
AU  - Isenović, Esma R.
AU  - Popovska-Percinic, Florina
AU  - Milošević, Verica
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/535
AB  - Thyroid C-cells produce calcitonin (CT), a hypocalcemic hormone, that acts as an inhibitor of bone resorption. In this study, we investigated the effects of tamoxifen (TAM) as a selective estrogen receptor modulator on thyroid C-cells, trabecular bone and biochemical markers of bone metabolism in an animal model of androgen deficiency, represented by middle-aged orchidectomized (Orx) rats. Fifteen-month-old male Wistar rats were divided into: Orx and sham-operated (SO) groups. Rats from one Orx group were injected subcutaneously with TAM citrate (Orx + TAM; 0.3 mgkg(-1) b.w.), while the rats from SO and a second Orx group received vehicle alone, once a day for 3 weeks. The peroxidase-antiperoxidase method was applied for localization of CT in C-cells. Thyroid C-cells were morphometrically and ultrastructurally analyzed. An ImageJ image-processing program was used to measure bone histomorphometric parameters. Blood serum samples were analyzed for CT, osteocalcin (OC), calcium (Ca2+) and phosphorus (P). Urinary Ca2+ concentrations were measured. TAM treatment significantly increased thyroid C-cell volume (V-c) and serum CT when compared with vehicle-treated Orx rats. Analysis of trabecular microarchitecture of the tibia showed that administration of TAM significantly increased cancellous bone area, trabecular thickness and trabecular number, whereas trabecular separation was significantly decreased compared with vehicle-treated Orx rats. Serum OC and urinary Ca2+ concentrations were significantly lower in comparison with the control Orx group. These results indicate that in our rat model of androgen deficiency, TAM stimulated calcitonin-producing thyroid C-cells and increased trabecular bone mass.
T2  - Journal of Anatomy
T1  - Tamoxifen stimulates calcitonin-producing thyroid C-cells and prevents trabecular bone loss in a rat model of androgen deficiency
VL  - 226
IS  - 5
SP  - 489
EP  - 496
DO  - 10.1111/joa.12298
ER  - 

@article{
author = "Filipović, Branko and Šošić-Jurjević, Branka and Ajdžanovic, Vladimir and Živanović, Jasmina and Isenović, Esma R. and Popovska-Percinic, Florina and Milošević, Verica",
year = "2015",
abstract = "Thyroid C-cells produce calcitonin (CT), a hypocalcemic hormone, that acts as an inhibitor of bone resorption. In this study, we investigated the effects of tamoxifen (TAM) as a selective estrogen receptor modulator on thyroid C-cells, trabecular bone and biochemical markers of bone metabolism in an animal model of androgen deficiency, represented by middle-aged orchidectomized (Orx) rats. Fifteen-month-old male Wistar rats were divided into: Orx and sham-operated (SO) groups. Rats from one Orx group were injected subcutaneously with TAM citrate (Orx + TAM; 0.3 mgkg(-1) b.w.), while the rats from SO and a second Orx group received vehicle alone, once a day for 3 weeks. The peroxidase-antiperoxidase method was applied for localization of CT in C-cells. Thyroid C-cells were morphometrically and ultrastructurally analyzed. An ImageJ image-processing program was used to measure bone histomorphometric parameters. Blood serum samples were analyzed for CT, osteocalcin (OC), calcium (Ca2+) and phosphorus (P). Urinary Ca2+ concentrations were measured. TAM treatment significantly increased thyroid C-cell volume (V-c) and serum CT when compared with vehicle-treated Orx rats. Analysis of trabecular microarchitecture of the tibia showed that administration of TAM significantly increased cancellous bone area, trabecular thickness and trabecular number, whereas trabecular separation was significantly decreased compared with vehicle-treated Orx rats. Serum OC and urinary Ca2+ concentrations were significantly lower in comparison with the control Orx group. These results indicate that in our rat model of androgen deficiency, TAM stimulated calcitonin-producing thyroid C-cells and increased trabecular bone mass.",
journal = "Journal of Anatomy",
title = "Tamoxifen stimulates calcitonin-producing thyroid C-cells and prevents trabecular bone loss in a rat model of androgen deficiency",
volume = "226",
number = "5",
pages = "489-496",
doi = "10.1111/joa.12298"
}

Filipović, B., Šošić-Jurjević, B., Ajdžanovic, V., Živanović, J., Isenović, E. R., Popovska-Percinic, F.,& Milošević, V.. (2015). Tamoxifen stimulates calcitonin-producing thyroid C-cells and prevents trabecular bone loss in a rat model of androgen deficiency. in Journal of Anatomy, 226(5), 489-496.
https://doi.org/10.1111/joa.12298

Filipović B, Šošić-Jurjević B, Ajdžanovic V, Živanović J, Isenović ER, Popovska-Percinic F, Milošević V. Tamoxifen stimulates calcitonin-producing thyroid C-cells and prevents trabecular bone loss in a rat model of androgen deficiency. in Journal of Anatomy. 2015;226(5):489-496.
doi:10.1111/joa.12298 .

Filipović, Branko, Šošić-Jurjević, Branka, Ajdžanovic, Vladimir, Živanović, Jasmina, Isenović, Esma R., Popovska-Percinic, Florina, Milošević, Verica, "Tamoxifen stimulates calcitonin-producing thyroid C-cells and prevents trabecular bone loss in a rat model of androgen deficiency" in Journal of Anatomy, 226, no. 5 (2015):489-496,
https://doi.org/10.1111/joa.12298 . .