TY  - JOUR
AU  - Vrbjar, Norbert
AU  - Vlkovicova, Jana
AU  - Snurikova, Denisa
AU  - Kalocayova, Barbora
AU  - Zorad, Stefan
AU  - Ćulafić, Tijana
AU  - Tepavčević, Snežana
AU  - Tothova, Lubomira
AU  - Radosinska, Dominika
AU  - Kollarova, Marta
AU  - Radosinska, Jana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10930
AB  - The study aimed to characterize the consequences of a 15-week intake of 10% fructose on the kidney, with the focus on oxidative stress markers and properties of the Na,K-ATPase enzyme. Various antioxidants naturally occurring in common food were demonstrated to be protective against fructose-induced deterioration of kidneys. Therefore, we also aimed to observe the effect of 6-week quercetin administration (20 mg/kg/day) that was initiated following the 9-week period of higher fructose intake, by determining the concentration of sodium, potassium, creatinine, urea, and glucose in blood plasma and oxidative status directly in the renal tissue. Kinetic studies of renal Na,K-ATPase were utilized for a deeper insight into the molecular principles of expected changes in this enzyme activity under conditions of presumed fructose-induced renal injury. Fructose intake led to increase in body weight gain, plasma glucose and sodium levels, and deterioration of kidney properties, although some compensatory mechanisms were observable. Quercetin administration improved glycemic control in rats exposed to fructose overload. However, an increase in plasma creatinine, a decrease in GSH/GSSG ratio in renal tissue homogenate, and a controversial effect on renal Na,K-ATPase enzyme suggest that quercetin treatment may not be beneficial in the condition of pre-existing renal pathology.
T2  - Life
T1  - Alterations in Oxidative Stress Markers and Na,K-ATPase Enzyme Properties in Kidney after Fructose Intake and Quercetin Intervention in Rats
VL  - 13
IS  - 4
SP  - 931
DO  - 10.3390/life13040931
ER  - 

@article{
author = "Vrbjar, Norbert and Vlkovicova, Jana and Snurikova, Denisa and Kalocayova, Barbora and Zorad, Stefan and Ćulafić, Tijana and Tepavčević, Snežana and Tothova, Lubomira and Radosinska, Dominika and Kollarova, Marta and Radosinska, Jana",
year = "2023",
abstract = "The study aimed to characterize the consequences of a 15-week intake of 10% fructose on the kidney, with the focus on oxidative stress markers and properties of the Na,K-ATPase enzyme. Various antioxidants naturally occurring in common food were demonstrated to be protective against fructose-induced deterioration of kidneys. Therefore, we also aimed to observe the effect of 6-week quercetin administration (20 mg/kg/day) that was initiated following the 9-week period of higher fructose intake, by determining the concentration of sodium, potassium, creatinine, urea, and glucose in blood plasma and oxidative status directly in the renal tissue. Kinetic studies of renal Na,K-ATPase were utilized for a deeper insight into the molecular principles of expected changes in this enzyme activity under conditions of presumed fructose-induced renal injury. Fructose intake led to increase in body weight gain, plasma glucose and sodium levels, and deterioration of kidney properties, although some compensatory mechanisms were observable. Quercetin administration improved glycemic control in rats exposed to fructose overload. However, an increase in plasma creatinine, a decrease in GSH/GSSG ratio in renal tissue homogenate, and a controversial effect on renal Na,K-ATPase enzyme suggest that quercetin treatment may not be beneficial in the condition of pre-existing renal pathology.",
journal = "Life",
title = "Alterations in Oxidative Stress Markers and Na,K-ATPase Enzyme Properties in Kidney after Fructose Intake and Quercetin Intervention in Rats",
volume = "13",
number = "4",
pages = "931",
doi = "10.3390/life13040931"
}

Vrbjar, N., Vlkovicova, J., Snurikova, D., Kalocayova, B., Zorad, S., Ćulafić, T., Tepavčević, S., Tothova, L., Radosinska, D., Kollarova, M.,& Radosinska, J.. (2023). Alterations in Oxidative Stress Markers and Na,K-ATPase Enzyme Properties in Kidney after Fructose Intake and Quercetin Intervention in Rats. in Life, 13(4), 931.
https://doi.org/10.3390/life13040931

Vrbjar N, Vlkovicova J, Snurikova D, Kalocayova B, Zorad S, Ćulafić T, Tepavčević S, Tothova L, Radosinska D, Kollarova M, Radosinska J. Alterations in Oxidative Stress Markers and Na,K-ATPase Enzyme Properties in Kidney after Fructose Intake and Quercetin Intervention in Rats. in Life. 2023;13(4):931.
doi:10.3390/life13040931 .

Vrbjar, Norbert, Vlkovicova, Jana, Snurikova, Denisa, Kalocayova, Barbora, Zorad, Stefan, Ćulafić, Tijana, Tepavčević, Snežana, Tothova, Lubomira, Radosinska, Dominika, Kollarova, Marta, Radosinska, Jana, "Alterations in Oxidative Stress Markers and Na,K-ATPase Enzyme Properties in Kidney after Fructose Intake and Quercetin Intervention in Rats" in Life, 13, no. 4 (2023):931,
https://doi.org/10.3390/life13040931 . .

TY  - JOUR
AU  - Romić, Snježana Đ.
AU  - Krskova, Katarina
AU  - Olszanecki, Rafal
AU  - Balazova, Lucia
AU  - Lory, Viktoria
AU  - Korićanac, Goran
AU  - Slamkova, Miroslava
AU  - Zorad, Stefan
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1764
AB  - Fatty acid (FA) uptake and/or intramuscular triglyceride (TG) accumulation in skeletal muscle are increased in obesity, type 2 diabetes and aging. FA translocase (FAT/CD36) translocation, lipin-1 subcellular localization and nuclear factor kappa B (NF-kappa B) p65 protein content in quadriceps muscle of young and old obese Zuckerfa/fa rats and their lean controls were analyzed by immunoblot to define obesity- and aging-related alterations in FA uptake, their subsequent metabolic fate and potential to activate pro-inflammatory signaling. As expected, obesity increased FAT/CD36 content in plasma membrane in quadriceps muscle of fa/fa rats. Aging increased cytosolic lipin-1 content in both, obese rats and their lean controls. Also, old obese rats had decreased level of nuclear extract lipin-lcompared to that in old lean rats. Neither obesity nor age altered NF-kappa B p65 protein content in cytosol and nuclear extract of quadriceps muscle suggesting that obesity/aging-induced changes in FA handling are not accompanied by NF-kappa B-mediated inflammation. Increase in plasma membrane FAT/CD36 content in obese rats and failure in lipin-1 export to nucleus with progression of obesity, implying an increase in FA uptake and their different channeling into lipid intermediates synthesis pathway in old fa/fa rats versus FA usage in lean rats of the same age.
T2  - General Physiology and Biophysics
T1  - Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats
VL  - 36
IS  - 4
SP  - 399
EP  - 406
DO  - 10.4149/gpb_2017010
ER  - 

@article{
author = "Romić, Snježana Đ. and Krskova, Katarina and Olszanecki, Rafal and Balazova, Lucia and Lory, Viktoria and Korićanac, Goran and Slamkova, Miroslava and Zorad, Stefan",
year = "2017",
abstract = "Fatty acid (FA) uptake and/or intramuscular triglyceride (TG) accumulation in skeletal muscle are increased in obesity, type 2 diabetes and aging. FA translocase (FAT/CD36) translocation, lipin-1 subcellular localization and nuclear factor kappa B (NF-kappa B) p65 protein content in quadriceps muscle of young and old obese Zuckerfa/fa rats and their lean controls were analyzed by immunoblot to define obesity- and aging-related alterations in FA uptake, their subsequent metabolic fate and potential to activate pro-inflammatory signaling. As expected, obesity increased FAT/CD36 content in plasma membrane in quadriceps muscle of fa/fa rats. Aging increased cytosolic lipin-1 content in both, obese rats and their lean controls. Also, old obese rats had decreased level of nuclear extract lipin-lcompared to that in old lean rats. Neither obesity nor age altered NF-kappa B p65 protein content in cytosol and nuclear extract of quadriceps muscle suggesting that obesity/aging-induced changes in FA handling are not accompanied by NF-kappa B-mediated inflammation. Increase in plasma membrane FAT/CD36 content in obese rats and failure in lipin-1 export to nucleus with progression of obesity, implying an increase in FA uptake and their different channeling into lipid intermediates synthesis pathway in old fa/fa rats versus FA usage in lean rats of the same age.",
journal = "General Physiology and Biophysics",
title = "Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats",
volume = "36",
number = "4",
pages = "399-406",
doi = "10.4149/gpb_2017010"
}

Romić, S. Đ., Krskova, K., Olszanecki, R., Balazova, L., Lory, V., Korićanac, G., Slamkova, M.,& Zorad, S.. (2017). Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats. in General Physiology and Biophysics, 36(4), 399-406.
https://doi.org/10.4149/gpb_2017010

Romić SĐ, Krskova K, Olszanecki R, Balazova L, Lory V, Korićanac G, Slamkova M, Zorad S. Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats. in General Physiology and Biophysics. 2017;36(4):399-406.
doi:10.4149/gpb_2017010 .

Romić, Snježana Đ., Krskova, Katarina, Olszanecki, Rafal, Balazova, Lucia, Lory, Viktoria, Korićanac, Goran, Slamkova, Miroslava, Zorad, Stefan, "Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats" in General Physiology and Biophysics, 36, no. 4 (2017):399-406,
https://doi.org/10.4149/gpb_2017010 . .