TY  - JOUR
AU  - Ivanović, Vesna
AU  - Dedović-Tanić, Nasta
AU  - Milovanović, Zorka M.
AU  - Lukić, Silvana
AU  - Nikolić, Srđan
AU  - Baltić, Vladimir
AU  - Stojiljković, Bratislav
AU  - Demajo, Miroslav
AU  - Mandušić, Vesna
AU  - Dimitrijević, Bogomir B.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1327
AB  - We present herein a case report style article on a rare advanced triple-negative breast cancer (TNBC) patient with 6-month disease-free interval, and 10-month overall survival. Our results demonstrate that the poor clinical outcome of this patient was associated with pronounced, more than fivefold higher, overexpression of both cFOS and TGF-beta 1 proteins in its metastatic nodal tissue extracts, when compared with the values of the two non-TNBC controls (with zero disease-free interval and overall survival). This original observation suggests, for the first time, that both the cFOS and TGF-beta 1 may be considered as a pair of biomarkers for an early assessment of poor prognosis for TNBC patients. The possible clinical implication of this observation is discussed.
T2  - Personalized Medicine
T1  - Case with triple-negative breast cancer shows overexpression of both cFOS and TGF-beta 1 in node-positive tissue
VL  - 13
IS  - 6
SP  - 523
EP  - 530
DO  - 10.2217/pme-2016-0032
ER  - 

@article{
author = "Ivanović, Vesna and Dedović-Tanić, Nasta and Milovanović, Zorka M. and Lukić, Silvana and Nikolić, Srđan and Baltić, Vladimir and Stojiljković, Bratislav and Demajo, Miroslav and Mandušić, Vesna and Dimitrijević, Bogomir B.",
year = "2016",
abstract = "We present herein a case report style article on a rare advanced triple-negative breast cancer (TNBC) patient with 6-month disease-free interval, and 10-month overall survival. Our results demonstrate that the poor clinical outcome of this patient was associated with pronounced, more than fivefold higher, overexpression of both cFOS and TGF-beta 1 proteins in its metastatic nodal tissue extracts, when compared with the values of the two non-TNBC controls (with zero disease-free interval and overall survival). This original observation suggests, for the first time, that both the cFOS and TGF-beta 1 may be considered as a pair of biomarkers for an early assessment of poor prognosis for TNBC patients. The possible clinical implication of this observation is discussed.",
journal = "Personalized Medicine",
title = "Case with triple-negative breast cancer shows overexpression of both cFOS and TGF-beta 1 in node-positive tissue",
volume = "13",
number = "6",
pages = "523-530",
doi = "10.2217/pme-2016-0032"
}

Ivanović, V., Dedović-Tanić, N., Milovanović, Z. M., Lukić, S., Nikolić, S., Baltić, V., Stojiljković, B., Demajo, M., Mandušić, V.,& Dimitrijević, B. B.. (2016). Case with triple-negative breast cancer shows overexpression of both cFOS and TGF-beta 1 in node-positive tissue. in Personalized Medicine, 13(6), 523-530.
https://doi.org/10.2217/pme-2016-0032

Ivanović V, Dedović-Tanić N, Milovanović ZM, Lukić S, Nikolić S, Baltić V, Stojiljković B, Demajo M, Mandušić V, Dimitrijević BB. Case with triple-negative breast cancer shows overexpression of both cFOS and TGF-beta 1 in node-positive tissue. in Personalized Medicine. 2016;13(6):523-530.
doi:10.2217/pme-2016-0032 .

Ivanović, Vesna, Dedović-Tanić, Nasta, Milovanović, Zorka M., Lukić, Silvana, Nikolić, Srđan, Baltić, Vladimir, Stojiljković, Bratislav, Demajo, Miroslav, Mandušić, Vesna, Dimitrijević, Bogomir B., "Case with triple-negative breast cancer shows overexpression of both cFOS and TGF-beta 1 in node-positive tissue" in Personalized Medicine, 13, no. 6 (2016):523-530,
https://doi.org/10.2217/pme-2016-0032 . .

TY  - JOUR
AU  - Ivanović, Vesna
AU  - Dedović-Tanić, Nasta
AU  - Milovanović, Zorka M.
AU  - Lukić, Silvana
AU  - Nikolić, Srđan
AU  - Baltić, Vladimir
AU  - Stojiljković, Bratislav
AU  - Budisin, Nikola
AU  - Savovski, Kiril
AU  - Demajo, Miroslav
AU  - Dimitrijević, Bogomir B.
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2728
AB  - OBJECTIVE: To use cytoplasmic tissue extract as a new specimen source to quantify transforming growth factor beta 1 (TGF beta 1) protein in metastatic axillary lymph node tissue (ALNT) of breast cancer (BC) patients and to confirm the feasibility of this approach in a prospective pilot study on a subgroup of patients with invasive BC. STUDY DESIGN: The 6 selected malignant and autologous nonmalignant pairs of ALNT were fractionated, under special preanalytical, nonaggressive/nondenaturing conditions, to obtain respective cytoplasmic extracts for TGF beta 1 detection by the Quantikine (R and D Systems Inc., Minneapolis, Minnesota, U.S.A.) enzyme-linked immunosorbent assay kit. RESULTS: The data indicated a highly significant (r=0.973054) positive linear correlation between the TGF beta 1 concentration and total protein concentration in cytoplasmic extract of metastatic ALNT. The subsequent patients pilot study, performed strictly before any clinicopathologic factors were accessible, revealed significantly (p LT 0.01) elevated TGF beta 1 in malignant ALNT (median value: 1.05 ng/mg protein, range: 0.67-3.6 ng/mg protein, n=6) vs. autologous nonmalignant ALNT controls (median value: 0.48 ng/mg protein, range: 0.29-0.90 ng/mg protein, n=6). This elevation was correlated with the number of metastatic axillary lymph nodes with respect to the total and was consistent with an increase in size of tumor deposits in axillary lymph nodes. CONCLUSION: Our data provide for the first time suggestive evidence that the TGF beta 1 level in cytoplasmic extracts of metastatic ALNTs may be a promising bio-marker of invasiveness for BC patients. Confirmatory, large-scale studies are needed to evaluate possible implications of this putative biomarker in BC diagnosis and treatment. (Anal Quant Cytol Histol 2009;31:288-295)
T2  - Analytical and Quantitative Cytology and Histology
T1  - Quantification of Transforming Growth Factor Beta 1 Levels in Metastatic Axillary Lymph Node Tissue Extracts from Breast Cancer Patients A New Specimen Source
VL  - 31
IS  - 5
SP  - 288
EP  - 295
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2728
ER  - 

@article{
author = "Ivanović, Vesna and Dedović-Tanić, Nasta and Milovanović, Zorka M. and Lukić, Silvana and Nikolić, Srđan and Baltić, Vladimir and Stojiljković, Bratislav and Budisin, Nikola and Savovski, Kiril and Demajo, Miroslav and Dimitrijević, Bogomir B.",
year = "2009",
abstract = "OBJECTIVE: To use cytoplasmic tissue extract as a new specimen source to quantify transforming growth factor beta 1 (TGF beta 1) protein in metastatic axillary lymph node tissue (ALNT) of breast cancer (BC) patients and to confirm the feasibility of this approach in a prospective pilot study on a subgroup of patients with invasive BC. STUDY DESIGN: The 6 selected malignant and autologous nonmalignant pairs of ALNT were fractionated, under special preanalytical, nonaggressive/nondenaturing conditions, to obtain respective cytoplasmic extracts for TGF beta 1 detection by the Quantikine (R and D Systems Inc., Minneapolis, Minnesota, U.S.A.) enzyme-linked immunosorbent assay kit. RESULTS: The data indicated a highly significant (r=0.973054) positive linear correlation between the TGF beta 1 concentration and total protein concentration in cytoplasmic extract of metastatic ALNT. The subsequent patients pilot study, performed strictly before any clinicopathologic factors were accessible, revealed significantly (p LT 0.01) elevated TGF beta 1 in malignant ALNT (median value: 1.05 ng/mg protein, range: 0.67-3.6 ng/mg protein, n=6) vs. autologous nonmalignant ALNT controls (median value: 0.48 ng/mg protein, range: 0.29-0.90 ng/mg protein, n=6). This elevation was correlated with the number of metastatic axillary lymph nodes with respect to the total and was consistent with an increase in size of tumor deposits in axillary lymph nodes. CONCLUSION: Our data provide for the first time suggestive evidence that the TGF beta 1 level in cytoplasmic extracts of metastatic ALNTs may be a promising bio-marker of invasiveness for BC patients. Confirmatory, large-scale studies are needed to evaluate possible implications of this putative biomarker in BC diagnosis and treatment. (Anal Quant Cytol Histol 2009;31:288-295)",
journal = "Analytical and Quantitative Cytology and Histology",
title = "Quantification of Transforming Growth Factor Beta 1 Levels in Metastatic Axillary Lymph Node Tissue Extracts from Breast Cancer Patients A New Specimen Source",
volume = "31",
number = "5",
pages = "288-295",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2728"
}

Ivanović, V., Dedović-Tanić, N., Milovanović, Z. M., Lukić, S., Nikolić, S., Baltić, V., Stojiljković, B., Budisin, N., Savovski, K., Demajo, M.,& Dimitrijević, B. B.. (2009). Quantification of Transforming Growth Factor Beta 1 Levels in Metastatic Axillary Lymph Node Tissue Extracts from Breast Cancer Patients A New Specimen Source. in Analytical and Quantitative Cytology and Histology, 31(5), 288-295.
https://hdl.handle.net/21.15107/rcub_vinar_2728

Ivanović V, Dedović-Tanić N, Milovanović ZM, Lukić S, Nikolić S, Baltić V, Stojiljković B, Budisin N, Savovski K, Demajo M, Dimitrijević BB. Quantification of Transforming Growth Factor Beta 1 Levels in Metastatic Axillary Lymph Node Tissue Extracts from Breast Cancer Patients A New Specimen Source. in Analytical and Quantitative Cytology and Histology. 2009;31(5):288-295.
https://hdl.handle.net/21.15107/rcub_vinar_2728 .

Ivanović, Vesna, Dedović-Tanić, Nasta, Milovanović, Zorka M., Lukić, Silvana, Nikolić, Srđan, Baltić, Vladimir, Stojiljković, Bratislav, Budisin, Nikola, Savovski, Kiril, Demajo, Miroslav, Dimitrijević, Bogomir B., "Quantification of Transforming Growth Factor Beta 1 Levels in Metastatic Axillary Lymph Node Tissue Extracts from Breast Cancer Patients A New Specimen Source" in Analytical and Quantitative Cytology and Histology, 31, no. 5 (2009):288-295,
https://hdl.handle.net/21.15107/rcub_vinar_2728 .