TY  - JOUR
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Petrović, Ivan M.
AU  - Valastro, Lucia M.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Ristić-Fira, Aleksandra
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4021
AB  - Background: Metastatic melanoma is one of the most aggressive tumours and is also very resistant to current therapeutic approaches. The aim of this investigation was the in vitro study of the anti-proliferative effects of fotemustine (FM; 100 and 250 mu M), bevacizumab (5 mu g/ml) and proton irradiation (12 and 16 Gy) on resistant HTB140 human melanoma cells. Methods: Viability was estimated by sulphorhodamine B assay, while cell proliferation was analyzed by 5-bromo-2-deoxyuridine assay. Cell cycle distribution and apoptosis were examined using flow cytometry. Results: Cell viability and proliferation were reduced after all applied treatments. The level of apoptosis significantly increased after treatment with FM, protons or a combination of all agents, while the apoptotic index ranged from 1.2 to 9.2. Proton irradiation, as well as combined treatment with bevacizumab and protons or 100 mu M FM, bevacizumab and protons, have reduced melanoma cell proliferation through the induction of G1 phase arrest. Single FM (250 mu M) or bevacizumab treatment and their combination, as well as the joint application of these 2 agents with protons, reduced cell proliferation and provoked G2 phase accumulation. Conclusion: The analyzed treatments reduced cell viability and proliferation, triggered G1 or G2 cell cycle phase accumulation and stimulated apoptotic cell death. Copyright (C) 2010 S. Karger AG, Basel
T2  - Chemotherapy
T1  - Anti-Tumour Activity of Fotemustine and Protons in Combination with Bevacizumab
VL  - 56
IS  - 3
SP  - 214
EP  - 222
DO  - 10.1159/000316333
ER  - 

@article{
author = "Korićanac, Lela and Žakula, Jelena and Petrović, Ivan M. and Valastro, Lucia M. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Ristić-Fira, Aleksandra",
year = "2010",
abstract = "Background: Metastatic melanoma is one of the most aggressive tumours and is also very resistant to current therapeutic approaches. The aim of this investigation was the in vitro study of the anti-proliferative effects of fotemustine (FM; 100 and 250 mu M), bevacizumab (5 mu g/ml) and proton irradiation (12 and 16 Gy) on resistant HTB140 human melanoma cells. Methods: Viability was estimated by sulphorhodamine B assay, while cell proliferation was analyzed by 5-bromo-2-deoxyuridine assay. Cell cycle distribution and apoptosis were examined using flow cytometry. Results: Cell viability and proliferation were reduced after all applied treatments. The level of apoptosis significantly increased after treatment with FM, protons or a combination of all agents, while the apoptotic index ranged from 1.2 to 9.2. Proton irradiation, as well as combined treatment with bevacizumab and protons or 100 mu M FM, bevacizumab and protons, have reduced melanoma cell proliferation through the induction of G1 phase arrest. Single FM (250 mu M) or bevacizumab treatment and their combination, as well as the joint application of these 2 agents with protons, reduced cell proliferation and provoked G2 phase accumulation. Conclusion: The analyzed treatments reduced cell viability and proliferation, triggered G1 or G2 cell cycle phase accumulation and stimulated apoptotic cell death. Copyright (C) 2010 S. Karger AG, Basel",
journal = "Chemotherapy",
title = "Anti-Tumour Activity of Fotemustine and Protons in Combination with Bevacizumab",
volume = "56",
number = "3",
pages = "214-222",
doi = "10.1159/000316333"
}

Korićanac, L., Žakula, J., Petrović, I. M., Valastro, L. M., Cirrone, G. A. P., Cuttone, G.,& Ristić-Fira, A.. (2010). Anti-Tumour Activity of Fotemustine and Protons in Combination with Bevacizumab. in Chemotherapy, 56(3), 214-222.
https://doi.org/10.1159/000316333

Korićanac L, Žakula J, Petrović IM, Valastro LM, Cirrone GAP, Cuttone G, Ristić-Fira A. Anti-Tumour Activity of Fotemustine and Protons in Combination with Bevacizumab. in Chemotherapy. 2010;56(3):214-222.
doi:10.1159/000316333 .

Korićanac, Lela, Žakula, Jelena, Petrović, Ivan M., Valastro, Lucia M., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Ristić-Fira, Aleksandra, "Anti-Tumour Activity of Fotemustine and Protons in Combination with Bevacizumab" in Chemotherapy, 56, no. 3 (2010):214-222,
https://doi.org/10.1159/000316333 . .

TY  - JOUR
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
AU  - Korićanac, Lela
AU  - Valastro, Lucia
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4101
AB  - Purpose: To analyse changes of cell inactivation and proliferation under therapeutic irradiation conditions along the proton spread out Bragg peak (SOBP) with particular emphasis on its distal declining edge. Materials and methods: HTB140 cells were irradiated at four positions: plateau, middle, distal end and distal declining edge of the 62 MeV proton SOBP. Doses ranged from 2-16 Gy. They were normalised in the middle of SOBP and delivered following the axial physical dose profile. Survival, proliferation and cell cycle were assessed seven days after irradiation. Results: Moving from proximal to distal irradiation position surviving fractions at 2 Gy (SF2) decreased from 0.88-0.59. Increased radiosensitivity of the cells was noticed for the doses below 4 Gy, resulting in two gradients of cell inactivation, stronger for lower and weaker for higher doses. Relative biological effectiveness (RBE) increased from 1.68-2.84 at the distal end of SOBP. A further rise of RBE reaching 7.14 was at its distal declining edge. Following the axial physical dose profile of SOBP the strongest inactivation was attained at its distal end and was comparable to that at its declining edge. Conclusions: Survival data confirmed very high radioresistance of HTB140 cells. An effect similar to low-dose hyper radiosensitivity (HRS) was observed for order of magnitude larger doses. Better response of cells to protons than to gamma-rays was illustrated by rather high RBE. Strong killing ability at the SOBP distal declining edge was the consequence of increasing proton linear energy transfer.
T2  - International Journal of Radiation Biology
T1  - Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak
VL  - 86
IS  - 9
SP  - 742
EP  - 751
DO  - 10.3109/09553002.2010.481322
ER  - 

@article{
author = "Petrović, Ivan M. and Ristić-Fira, Aleksandra and Todorović, Danijela V. and Korićanac, Lela and Valastro, Lucia and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo",
year = "2010",
abstract = "Purpose: To analyse changes of cell inactivation and proliferation under therapeutic irradiation conditions along the proton spread out Bragg peak (SOBP) with particular emphasis on its distal declining edge. Materials and methods: HTB140 cells were irradiated at four positions: plateau, middle, distal end and distal declining edge of the 62 MeV proton SOBP. Doses ranged from 2-16 Gy. They were normalised in the middle of SOBP and delivered following the axial physical dose profile. Survival, proliferation and cell cycle were assessed seven days after irradiation. Results: Moving from proximal to distal irradiation position surviving fractions at 2 Gy (SF2) decreased from 0.88-0.59. Increased radiosensitivity of the cells was noticed for the doses below 4 Gy, resulting in two gradients of cell inactivation, stronger for lower and weaker for higher doses. Relative biological effectiveness (RBE) increased from 1.68-2.84 at the distal end of SOBP. A further rise of RBE reaching 7.14 was at its distal declining edge. Following the axial physical dose profile of SOBP the strongest inactivation was attained at its distal end and was comparable to that at its declining edge. Conclusions: Survival data confirmed very high radioresistance of HTB140 cells. An effect similar to low-dose hyper radiosensitivity (HRS) was observed for order of magnitude larger doses. Better response of cells to protons than to gamma-rays was illustrated by rather high RBE. Strong killing ability at the SOBP distal declining edge was the consequence of increasing proton linear energy transfer.",
journal = "International Journal of Radiation Biology",
title = "Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak",
volume = "86",
number = "9",
pages = "742-751",
doi = "10.3109/09553002.2010.481322"
}

Petrović, I. M., Ristić-Fira, A., Todorović, D. V., Korićanac, L., Valastro, L., Cirrone, G. A. P.,& Cuttone, G.. (2010). Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak. in International Journal of Radiation Biology, 86(9), 742-751.
https://doi.org/10.3109/09553002.2010.481322

Petrović IM, Ristić-Fira A, Todorović DV, Korićanac L, Valastro L, Cirrone GAP, Cuttone G. Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak. in International Journal of Radiation Biology. 2010;86(9):742-751.
doi:10.3109/09553002.2010.481322 .

Petrović, Ivan M., Ristić-Fira, Aleksandra, Todorović, Danijela V., Korićanac, Lela, Valastro, Lucia, Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, "Response of a radioresistant human melanoma cell line along the proton spread-out Bragg peak" in International Journal of Radiation Biology, 86, no. 9 (2010):742-751,
https://doi.org/10.3109/09553002.2010.481322 . .

TY  - JOUR
AU  - Ristić-Fira, Aleksandra
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Valastro, Lucia M.
AU  - Iannolo, Gioacchin
AU  - Privitera, Giuseppe
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3702
AB  - Background: Considering that HTB140 melanoma cells have shown a poor response to either protons or alkylating agents, the effects of a combined use of these agents have been analysed. Methods: Cells were irradiated in the middle of the therapeutic 62 MeV proton spread out Bragg peak (SOBP). Irradiation doses were 12 or 16 Gy and are those frequently used in proton therapy. Four days after irradiation cells were treated with fotemustine (FM) or dacarbazine (DTIC). Drug concentrations were 100 and 250 mu M, values close to those that produce 50% of growth inhibition. Cell viability, proliferation, survival and cell cycle distribution were assessed 7 days after irradiation that corresponds to more than six doubling times of HTB140 cells. In this way incubation periods providing the best single effects of drugs (3 days) and protons (7 days) coincided at the same time. Results: Single proton irradiations have reduced the number of cells to similar to 50%. FM caused stronger cell inactivation due to its high toxicity, while the effectiveness of DTIC, that was important at short term, almost vanished with the incubation of 7 days. Cellular mechanisms triggered by proton irradiation differently influenced the final effects of combined treatments. Combination of protons and FM did not improve cell inactivation level achieved by single treatments. A low efficiency of the single DTIC treatment was overcome when DTIC was introduced following proton irradiation, giving better inhibitory effects with respect to the single treatments. Most of the analysed cells were in G1/S phase, viable, active and able to replicate DNA. Conclusion: The obtained results are the consequence of a high resistance of HTB140 melanoma cells to protons and/or drugs. The inactivation level of the HTB140 human melanoma cells after protons, FM or DTIC treatments was not enhanced by their combined application.
T2  - Journal of Experimental and Clinical Cancer Research
T1  - Effects of fotemustine or dacarbasine on a melanoma cell line pretreated with therapeutic proton irradiation
VL  - 28
DO  - 10.1186/1756-9966-28-50
ER  - 

@article{
author = "Ristić-Fira, Aleksandra and Korićanac, Lela and Žakula, Jelena and Valastro, Lucia M. and Iannolo, Gioacchin and Privitera, Giuseppe and Cuttone, Giacomo and Petrović, Ivan M.",
year = "2009",
abstract = "Background: Considering that HTB140 melanoma cells have shown a poor response to either protons or alkylating agents, the effects of a combined use of these agents have been analysed. Methods: Cells were irradiated in the middle of the therapeutic 62 MeV proton spread out Bragg peak (SOBP). Irradiation doses were 12 or 16 Gy and are those frequently used in proton therapy. Four days after irradiation cells were treated with fotemustine (FM) or dacarbazine (DTIC). Drug concentrations were 100 and 250 mu M, values close to those that produce 50% of growth inhibition. Cell viability, proliferation, survival and cell cycle distribution were assessed 7 days after irradiation that corresponds to more than six doubling times of HTB140 cells. In this way incubation periods providing the best single effects of drugs (3 days) and protons (7 days) coincided at the same time. Results: Single proton irradiations have reduced the number of cells to similar to 50%. FM caused stronger cell inactivation due to its high toxicity, while the effectiveness of DTIC, that was important at short term, almost vanished with the incubation of 7 days. Cellular mechanisms triggered by proton irradiation differently influenced the final effects of combined treatments. Combination of protons and FM did not improve cell inactivation level achieved by single treatments. A low efficiency of the single DTIC treatment was overcome when DTIC was introduced following proton irradiation, giving better inhibitory effects with respect to the single treatments. Most of the analysed cells were in G1/S phase, viable, active and able to replicate DNA. Conclusion: The obtained results are the consequence of a high resistance of HTB140 melanoma cells to protons and/or drugs. The inactivation level of the HTB140 human melanoma cells after protons, FM or DTIC treatments was not enhanced by their combined application.",
journal = "Journal of Experimental and Clinical Cancer Research",
title = "Effects of fotemustine or dacarbasine on a melanoma cell line pretreated with therapeutic proton irradiation",
volume = "28",
doi = "10.1186/1756-9966-28-50"
}

Ristić-Fira, A., Korićanac, L., Žakula, J., Valastro, L. M., Iannolo, G., Privitera, G., Cuttone, G.,& Petrović, I. M.. (2009). Effects of fotemustine or dacarbasine on a melanoma cell line pretreated with therapeutic proton irradiation. in Journal of Experimental and Clinical Cancer Research, 28.
https://doi.org/10.1186/1756-9966-28-50

Ristić-Fira A, Korićanac L, Žakula J, Valastro LM, Iannolo G, Privitera G, Cuttone G, Petrović IM. Effects of fotemustine or dacarbasine on a melanoma cell line pretreated with therapeutic proton irradiation. in Journal of Experimental and Clinical Cancer Research. 2009;28.
doi:10.1186/1756-9966-28-50 .

Ristić-Fira, Aleksandra, Korićanac, Lela, Žakula, Jelena, Valastro, Lucia M., Iannolo, Gioacchin, Privitera, Giuseppe, Cuttone, Giacomo, Petrović, Ivan M., "Effects of fotemustine or dacarbasine on a melanoma cell line pretreated with therapeutic proton irradiation" in Journal of Experimental and Clinical Cancer Research, 28 (2009),
https://doi.org/10.1186/1756-9966-28-50 . .

TY  - CONF
AU  - Korićanac, Lela
AU  - Petrović, Ivan M.
AU  - Požega, J. J.
AU  - Valastro, Lucia M.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Ristić-Fira, Aleksandra
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6760
C3  - FEBS Journal
T1  - Cell cycle distribution and induction apoptosis after joint treatment with fotemustine and protons
VL  - 275
SP  - 409
EP  - 409
UR  - https://hdl.handle.net/21.15107/rcub_vinar_6760
ER  - 

@conference{
author = "Korićanac, Lela and Petrović, Ivan M. and Požega, J. J. and Valastro, Lucia M. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Ristić-Fira, Aleksandra",
year = "2008",
journal = "FEBS Journal",
title = "Cell cycle distribution and induction apoptosis after joint treatment with fotemustine and protons",
volume = "275",
pages = "409-409",
url = "https://hdl.handle.net/21.15107/rcub_vinar_6760"
}

Korićanac, L., Petrović, I. M., Požega, J. J., Valastro, L. M., Cirrone, G. A. P., Cuttone, G.,& Ristić-Fira, A.. (2008). Cell cycle distribution and induction apoptosis after joint treatment with fotemustine and protons. in FEBS Journal, 275, 409-409.
https://hdl.handle.net/21.15107/rcub_vinar_6760

Korićanac L, Petrović IM, Požega JJ, Valastro LM, Cirrone GAP, Cuttone G, Ristić-Fira A. Cell cycle distribution and induction apoptosis after joint treatment with fotemustine and protons. in FEBS Journal. 2008;275:409-409.
https://hdl.handle.net/21.15107/rcub_vinar_6760 .

Korićanac, Lela, Petrović, Ivan M., Požega, J. J., Valastro, Lucia M., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Ristić-Fira, Aleksandra, "Cell cycle distribution and induction apoptosis after joint treatment with fotemustine and protons" in FEBS Journal, 275 (2008):409-409,
https://hdl.handle.net/21.15107/rcub_vinar_6760 .

TY  - CONF
AU  - Požega, J. J.
AU  - Ristić-Fira, Aleksandra
AU  - Korićanac, Lela
AU  - Valastro, Lucia M.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6761
C3  - FEBS Journal
T1  - Apoptotic ability of carbon ions on a resistant melanoma cell line
VL  - 275
SP  - 425
EP  - 425
UR  - https://hdl.handle.net/21.15107/rcub_vinar_6761
ER  - 

@conference{
author = "Požega, J. J. and Ristić-Fira, Aleksandra and Korićanac, Lela and Valastro, Lucia M. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Petrović, Ivan M.",
year = "2008",
journal = "FEBS Journal",
title = "Apoptotic ability of carbon ions on a resistant melanoma cell line",
volume = "275",
pages = "425-425",
url = "https://hdl.handle.net/21.15107/rcub_vinar_6761"
}

Požega, J. J., Ristić-Fira, A., Korićanac, L., Valastro, L. M., Cirrone, G. A. P., Cuttone, G.,& Petrović, I. M.. (2008). Apoptotic ability of carbon ions on a resistant melanoma cell line. in FEBS Journal, 275, 425-425.
https://hdl.handle.net/21.15107/rcub_vinar_6761

Požega JJ, Ristić-Fira A, Korićanac L, Valastro LM, Cirrone GAP, Cuttone G, Petrović IM. Apoptotic ability of carbon ions on a resistant melanoma cell line. in FEBS Journal. 2008;275:425-425.
https://hdl.handle.net/21.15107/rcub_vinar_6761 .

Požega, J. J., Ristić-Fira, Aleksandra, Korićanac, Lela, Valastro, Lucia M., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Petrović, Ivan M., "Apoptotic ability of carbon ions on a resistant melanoma cell line" in FEBS Journal, 275 (2008):425-425,
https://hdl.handle.net/21.15107/rcub_vinar_6761 .

TY  - JOUR
AU  - Ristić-Fira, Aleksandra
AU  - Petrović, Ivan M.
AU  - Korićanac, Lela
AU  - Valastro, Lucia M.
AU  - Privitera, Giuseppe
AU  - Cuttone, Giacomo
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3613
AB  - The correlation between time dependent viabilities, after applying two radiation qualities and two alkylating agents on HTB140 melanoma cells, has been studied. Irradiations were performed with gamma-rays and 62 MeV protons, close to the Bragg peak maximum, delivering doses of 8-24 Gy. Treatments with fotemustine (FM) and dacarbazine (DTIC) were carried out with concentrations of 0.05-2 mM. High radio-resistance of HTB140 cells revealed by a clonogenic assay was confirmed by microtetrasolium and sulforhodamine B, through the surviving fraction at 2 Gy (SF2), being 0.961-0.956 for gamma-rays and 0.931-0.887 for protons. A better efficiency of protons was illustrated by relative biological effectiveness at 2 Gy (RBE), ranging from 1.69 to 1.89. A kinetic study of concentration dependent cytotoxicity indicated that the best effect of the drugs, estimated as the concentration that produces 50% of growth inhibition (IC(50)), was obtained at 48 h, having values of 76 mu M for DTIC and 145 mu M for FM. The cytostatic ability of the drugs pointed out that the presence of DTIC at 24 h, compared to FM, was insufficient to produce an effect. Protons and FM demonstrated their pro apoptotic capacity. Cross-resistance between treatments applied to the HTB140 cells was observed, protons being the most efficient, while DTIC, FM and gamma-rays demonstrated a lower level of cell inactivation. (C) 2008 Associazione Italiana di Fisica Medica. Published by Elsevier Ltd. ALL rights reserved.
T2  - Physica Medica
T1  - Assessment of the inhibitory effects of different radiation qualities or chemotherapeutic agents on a human melanoma cell line
VL  - 24
IS  - 4
SP  - 187
EP  - 195
DO  - 10.1016/j.ejmp.2008.04.002
ER  - 

@article{
author = "Ristić-Fira, Aleksandra and Petrović, Ivan M. and Korićanac, Lela and Valastro, Lucia M. and Privitera, Giuseppe and Cuttone, Giacomo",
year = "2008",
abstract = "The correlation between time dependent viabilities, after applying two radiation qualities and two alkylating agents on HTB140 melanoma cells, has been studied. Irradiations were performed with gamma-rays and 62 MeV protons, close to the Bragg peak maximum, delivering doses of 8-24 Gy. Treatments with fotemustine (FM) and dacarbazine (DTIC) were carried out with concentrations of 0.05-2 mM. High radio-resistance of HTB140 cells revealed by a clonogenic assay was confirmed by microtetrasolium and sulforhodamine B, through the surviving fraction at 2 Gy (SF2), being 0.961-0.956 for gamma-rays and 0.931-0.887 for protons. A better efficiency of protons was illustrated by relative biological effectiveness at 2 Gy (RBE), ranging from 1.69 to 1.89. A kinetic study of concentration dependent cytotoxicity indicated that the best effect of the drugs, estimated as the concentration that produces 50% of growth inhibition (IC(50)), was obtained at 48 h, having values of 76 mu M for DTIC and 145 mu M for FM. The cytostatic ability of the drugs pointed out that the presence of DTIC at 24 h, compared to FM, was insufficient to produce an effect. Protons and FM demonstrated their pro apoptotic capacity. Cross-resistance between treatments applied to the HTB140 cells was observed, protons being the most efficient, while DTIC, FM and gamma-rays demonstrated a lower level of cell inactivation. (C) 2008 Associazione Italiana di Fisica Medica. Published by Elsevier Ltd. ALL rights reserved.",
journal = "Physica Medica",
title = "Assessment of the inhibitory effects of different radiation qualities or chemotherapeutic agents on a human melanoma cell line",
volume = "24",
number = "4",
pages = "187-195",
doi = "10.1016/j.ejmp.2008.04.002"
}

Ristić-Fira, A., Petrović, I. M., Korićanac, L., Valastro, L. M., Privitera, G.,& Cuttone, G.. (2008). Assessment of the inhibitory effects of different radiation qualities or chemotherapeutic agents on a human melanoma cell line. in Physica Medica, 24(4), 187-195.
https://doi.org/10.1016/j.ejmp.2008.04.002

Ristić-Fira A, Petrović IM, Korićanac L, Valastro LM, Privitera G, Cuttone G. Assessment of the inhibitory effects of different radiation qualities or chemotherapeutic agents on a human melanoma cell line. in Physica Medica. 2008;24(4):187-195.
doi:10.1016/j.ejmp.2008.04.002 .

Ristić-Fira, Aleksandra, Petrović, Ivan M., Korićanac, Lela, Valastro, Lucia M., Privitera, Giuseppe, Cuttone, Giacomo, "Assessment of the inhibitory effects of different radiation qualities or chemotherapeutic agents on a human melanoma cell line" in Physica Medica, 24, no. 4 (2008):187-195,
https://doi.org/10.1016/j.ejmp.2008.04.002 . .

TY  - JOUR
AU  - Petrović, Ivan M.
AU  - Korićanac, Lela
AU  - Todorović, Danijela V.
AU  - Ristić-Fira, Aleksandra
AU  - Valastro, Lucia M.
AU  - Privitera, Giuseppe
AU  - Cuttone, Giacomo
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6647
AB  - Viability of human HTB140 melanoma cells after being exposed to fotemustine (FM) and dacarbazine (DTIC) as well as to proton irradiation was studied. Effects of 100 and 250 mu M drugs were assessed after incubation of 6, 24, 48, 72, and 96 h. Irradiations were performed with 62 MeV therapeutic protons, delivering to the cell monolayer single doses of 2, 4, 8, 12, and 16 Gy. Viability was evaluated 7 days after irradiation. Inactivation level was estimated using microtetrasolium (MTT) and sulforhodamine B (SRB) assays. Combined effects of each drug and protons, were carried out using the same drug concentrations. Proton doses applied were those used in therapy, that is, 12 and 16 Gy. With the increase of drug concentration or irradiation dose, level of cell inactivation reached approximately 60%, 48 h after drug treatment or 7 days after irradiation at 16 Gy. Considering the rate of drug concentrations used, as well as the level of doses applied, it appears that HTB140 cells are more resistant to proton irradiation than to alkylating agents tested. The combined treatment with FM or DTIC and protons did not show significant changes of cell viability as compared to the effects of single agents. Since the time point for measuring cumulative effects of drug and irradiation was 48 h post irradiation, it seems that the obtained level of viability could be attributed primarily to the effects of drugs.
T2  - Annals of the New York Academy of Sciences
T1  - Viability of a human melanoma cell after single and combined treatment with fotemustine, dacarbazine, and proton irradiation
VL  - 1095
SP  - 154
EP  - 164
DO  - 10.1196/annals.1397.019
ER  - 

@article{
author = "Petrović, Ivan M. and Korićanac, Lela and Todorović, Danijela V. and Ristić-Fira, Aleksandra and Valastro, Lucia M. and Privitera, Giuseppe and Cuttone, Giacomo",
year = "2007",
abstract = "Viability of human HTB140 melanoma cells after being exposed to fotemustine (FM) and dacarbazine (DTIC) as well as to proton irradiation was studied. Effects of 100 and 250 mu M drugs were assessed after incubation of 6, 24, 48, 72, and 96 h. Irradiations were performed with 62 MeV therapeutic protons, delivering to the cell monolayer single doses of 2, 4, 8, 12, and 16 Gy. Viability was evaluated 7 days after irradiation. Inactivation level was estimated using microtetrasolium (MTT) and sulforhodamine B (SRB) assays. Combined effects of each drug and protons, were carried out using the same drug concentrations. Proton doses applied were those used in therapy, that is, 12 and 16 Gy. With the increase of drug concentration or irradiation dose, level of cell inactivation reached approximately 60%, 48 h after drug treatment or 7 days after irradiation at 16 Gy. Considering the rate of drug concentrations used, as well as the level of doses applied, it appears that HTB140 cells are more resistant to proton irradiation than to alkylating agents tested. The combined treatment with FM or DTIC and protons did not show significant changes of cell viability as compared to the effects of single agents. Since the time point for measuring cumulative effects of drug and irradiation was 48 h post irradiation, it seems that the obtained level of viability could be attributed primarily to the effects of drugs.",
journal = "Annals of the New York Academy of Sciences",
title = "Viability of a human melanoma cell after single and combined treatment with fotemustine, dacarbazine, and proton irradiation",
volume = "1095",
pages = "154-164",
doi = "10.1196/annals.1397.019"
}

Petrović, I. M., Korićanac, L., Todorović, D. V., Ristić-Fira, A., Valastro, L. M., Privitera, G.,& Cuttone, G.. (2007). Viability of a human melanoma cell after single and combined treatment with fotemustine, dacarbazine, and proton irradiation. in Annals of the New York Academy of Sciences, 1095, 154-164.
https://doi.org/10.1196/annals.1397.019

Petrović IM, Korićanac L, Todorović DV, Ristić-Fira A, Valastro LM, Privitera G, Cuttone G. Viability of a human melanoma cell after single and combined treatment with fotemustine, dacarbazine, and proton irradiation. in Annals of the New York Academy of Sciences. 2007;1095:154-164.
doi:10.1196/annals.1397.019 .

Petrović, Ivan M., Korićanac, Lela, Todorović, Danijela V., Ristić-Fira, Aleksandra, Valastro, Lucia M., Privitera, Giuseppe, Cuttone, Giacomo, "Viability of a human melanoma cell after single and combined treatment with fotemustine, dacarbazine, and proton irradiation" in Annals of the New York Academy of Sciences, 1095 (2007):154-164,
https://doi.org/10.1196/annals.1397.019 . .

TY  - CONF
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
AU  - Korićanac, Lela
AU  - Petrović, Ivan M.
AU  - Valastro, Lucia M.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Raffaele, Luigi
AU  - Cuttone, Giacomo
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6648
AB  - Effects of single irradiation with gamma rays and protons on human HTB140 melanoma cell growth were compared. Exponentially growing cells were irradiated close to the Bragg peak maximum of the unmodulated 62 MeV protons, as well as with Co-60 gamma rays. Applied doses ranged from 8 to 24 Gy. Viability of cells and proliferation capacity were assessed 7 days after irradiation. Induction of apoptosis and cell cycle phase redistribution were observed 6 and 48 h after irradiation. Significant inhibitory effects of both irradiation qualities were detected 7 days after irradiation. Important reduction of HTB140 cell viability was observed after irradiation with protons. Almost linear and highly significant (P LT 0.001) decrease of cell proliferation was observed 7 days after irradiation with gamma rays and protons, as compared to nonirradiated controls. Protons induced apoptosis, both 6 and 48 h after irradiation. With the increase of post-irradiation incubation time, number of apoptotic cells decreased. Exposure of HTB140 cells to gamma rays did not provoke apoptotic cell death. Important number of cells in G1-S phase, detected by the cell cycle phase redistribution analyses, suggested high metabolic activity of irradiated melanoma cells within the first 48 h. Both irradiation qualities caused modest G2-M arrest 6 and 48 h after irradiation, thus supporting results that illustrated high radioresistance of HTB140 cells.
C3  - Annals of the New York Academy of Sciences
T1  - Response of a human melanoma cell line to low and high ionizing radiation
VL  - 1095
SP  - 165
EP  - 174
DO  - 10.1196/annals.1397.020
ER  - 

@conference{
author = "Ristić-Fira, Aleksandra and Todorović, Danijela V. and Korićanac, Lela and Petrović, Ivan M. and Valastro, Lucia M. and Cirrone, Giuseppe Antonio Pablo and Raffaele, Luigi and Cuttone, Giacomo",
year = "2007",
abstract = "Effects of single irradiation with gamma rays and protons on human HTB140 melanoma cell growth were compared. Exponentially growing cells were irradiated close to the Bragg peak maximum of the unmodulated 62 MeV protons, as well as with Co-60 gamma rays. Applied doses ranged from 8 to 24 Gy. Viability of cells and proliferation capacity were assessed 7 days after irradiation. Induction of apoptosis and cell cycle phase redistribution were observed 6 and 48 h after irradiation. Significant inhibitory effects of both irradiation qualities were detected 7 days after irradiation. Important reduction of HTB140 cell viability was observed after irradiation with protons. Almost linear and highly significant (P LT 0.001) decrease of cell proliferation was observed 7 days after irradiation with gamma rays and protons, as compared to nonirradiated controls. Protons induced apoptosis, both 6 and 48 h after irradiation. With the increase of post-irradiation incubation time, number of apoptotic cells decreased. Exposure of HTB140 cells to gamma rays did not provoke apoptotic cell death. Important number of cells in G1-S phase, detected by the cell cycle phase redistribution analyses, suggested high metabolic activity of irradiated melanoma cells within the first 48 h. Both irradiation qualities caused modest G2-M arrest 6 and 48 h after irradiation, thus supporting results that illustrated high radioresistance of HTB140 cells.",
journal = "Annals of the New York Academy of Sciences",
title = "Response of a human melanoma cell line to low and high ionizing radiation",
volume = "1095",
pages = "165-174",
doi = "10.1196/annals.1397.020"
}

Ristić-Fira, A., Todorović, D. V., Korićanac, L., Petrović, I. M., Valastro, L. M., Cirrone, G. A. P., Raffaele, L.,& Cuttone, G.. (2007). Response of a human melanoma cell line to low and high ionizing radiation. in Annals of the New York Academy of Sciences, 1095, 165-174.
https://doi.org/10.1196/annals.1397.020

Ristić-Fira A, Todorović DV, Korićanac L, Petrović IM, Valastro LM, Cirrone GAP, Raffaele L, Cuttone G. Response of a human melanoma cell line to low and high ionizing radiation. in Annals of the New York Academy of Sciences. 2007;1095:165-174.
doi:10.1196/annals.1397.020 .

Ristić-Fira, Aleksandra, Todorović, Danijela V., Korićanac, Lela, Petrović, Ivan M., Valastro, Lucia M., Cirrone, Giuseppe Antonio Pablo, Raffaele, Luigi, Cuttone, Giacomo, "Response of a human melanoma cell line to low and high ionizing radiation" in Annals of the New York Academy of Sciences, 1095 (2007):165-174,
https://doi.org/10.1196/annals.1397.020 . .

TY  - JOUR
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
AU  - Valastro, Lucia M.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
PY  - 2006
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3017
AB  - Purpose: To measure the ability of protons and gamma-rays to effect cell viability and cell survival of human HTB140 melanoma cells. Materials and methods: Exponentially growing HTB140 cells were irradiated close to the Bragg peak maximum of the 62 MeV protons or with Co-60 gamma-rays with single doses, ranging from 8-24 Gy. Cell viability using the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay was evaluated at 6 h, 24 h, 48 h or 7 days after irradiation and clonogenic survival was assessed at 7 days after irradiation. Cell cycle phase redistribution and the level of apoptosis were evaluated at 6 h and 48 h after irradiation. Results: The study of cell viability as a function of time (cell survival progression) and cell survival, using a clonal assay, demonstrated the considerably stronger inactivation effect of protons compared to gamma-rays with a relative biological effectiveness (RBE) of similar to 1.64. Cell cycle phase distribution and apoptosis levels with time enabled us to investigate the development and the character of the damage induced by irradiation. Due to the high radio-resistance of HTB140 cells, cell cycle phase redistribution exhibited only a modest cell accumulation in G2/M phase. Protons but not gamma-rays induced apoptosis. Conclusions: It appears that protons reduce the number of HTB140 cells by apoptosis as well as by severe DNA damage, while gamma-rays eliminate viable cells primarily by the production of irreparable DNA damage. Protons have an increased RBE relative to gamma-rays.
T2  - International Journal of Radiation Biology
T1  - Radiobiological analysis of human melanoma cells on the 62 MeV CATANA proton beam
VL  - 82
IS  - 4
SP  - 251
EP  - 265
DO  - 10.1080/09553000600669859
ER  - 

@article{
author = "Petrović, Ivan M. and Ristić-Fira, Aleksandra and Todorović, Danijela V. and Valastro, Lucia M. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo",
year = "2006",
abstract = "Purpose: To measure the ability of protons and gamma-rays to effect cell viability and cell survival of human HTB140 melanoma cells. Materials and methods: Exponentially growing HTB140 cells were irradiated close to the Bragg peak maximum of the 62 MeV protons or with Co-60 gamma-rays with single doses, ranging from 8-24 Gy. Cell viability using the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay was evaluated at 6 h, 24 h, 48 h or 7 days after irradiation and clonogenic survival was assessed at 7 days after irradiation. Cell cycle phase redistribution and the level of apoptosis were evaluated at 6 h and 48 h after irradiation. Results: The study of cell viability as a function of time (cell survival progression) and cell survival, using a clonal assay, demonstrated the considerably stronger inactivation effect of protons compared to gamma-rays with a relative biological effectiveness (RBE) of similar to 1.64. Cell cycle phase distribution and apoptosis levels with time enabled us to investigate the development and the character of the damage induced by irradiation. Due to the high radio-resistance of HTB140 cells, cell cycle phase redistribution exhibited only a modest cell accumulation in G2/M phase. Protons but not gamma-rays induced apoptosis. Conclusions: It appears that protons reduce the number of HTB140 cells by apoptosis as well as by severe DNA damage, while gamma-rays eliminate viable cells primarily by the production of irreparable DNA damage. Protons have an increased RBE relative to gamma-rays.",
journal = "International Journal of Radiation Biology",
title = "Radiobiological analysis of human melanoma cells on the 62 MeV CATANA proton beam",
volume = "82",
number = "4",
pages = "251-265",
doi = "10.1080/09553000600669859"
}

Petrović, I. M., Ristić-Fira, A., Todorović, D. V., Valastro, L. M., Cirrone, G. A. P.,& Cuttone, G.. (2006). Radiobiological analysis of human melanoma cells on the 62 MeV CATANA proton beam. in International Journal of Radiation Biology, 82(4), 251-265.
https://doi.org/10.1080/09553000600669859

Petrović IM, Ristić-Fira A, Todorović DV, Valastro LM, Cirrone GAP, Cuttone G. Radiobiological analysis of human melanoma cells on the 62 MeV CATANA proton beam. in International Journal of Radiation Biology. 2006;82(4):251-265.
doi:10.1080/09553000600669859 .

Petrović, Ivan M., Ristić-Fira, Aleksandra, Todorović, Danijela V., Valastro, Lucia M., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, "Radiobiological analysis of human melanoma cells on the 62 MeV CATANA proton beam" in International Journal of Radiation Biology, 82, no. 4 (2006):251-265,
https://doi.org/10.1080/09553000600669859 . .

TY  - CONF
AU  - Petrović, Ivan M.
AU  - Todorović, Danijela V.
AU  - Valastro, Lucia M.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Ristić-Fira, Aleksandra
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9506
AB  - The aim of this study was to determine the initial inactivation of cells induced by high-energy proton beam designed for the treatment of eye melanoma. Exponentially growing HTB140 cells were exposed to an unmodulated 62 MeV proton beam delivered over the single dose range from 8 Gy to 24 Gy. Position of samples was in the zone of the Bragg peak, having high LET values. Surviving fractions were evaluated at 6, 24 and 48 h post-irradiation. The survival curves exhibited a well-known shoulder, decreasing for doses higher than 8 Gy. Therefore, a significant dose dependent early cell inactivation after single delivery of 16 Gy to 24 Gy to the cell monolayer was observed. With the increase of the post-irradiation incubation time, a better killing effect, as the consequence of clonogenic survival, was detected.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
T1  - Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: I. survival of HTB140 melanoma cells
VL  - 1
SP  - 426
EP  - 428
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9506
ER  - 

@conference{
author = "Petrović, Ivan M. and Todorović, Danijela V. and Valastro, Lucia M. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Ristić-Fira, Aleksandra",
year = "2004",
abstract = "The aim of this study was to determine the initial inactivation of cells induced by high-energy proton beam designed for the treatment of eye melanoma. Exponentially growing HTB140 cells were exposed to an unmodulated 62 MeV proton beam delivered over the single dose range from 8 Gy to 24 Gy. Position of samples was in the zone of the Bragg peak, having high LET values. Surviving fractions were evaluated at 6, 24 and 48 h post-irradiation. The survival curves exhibited a well-known shoulder, decreasing for doses higher than 8 Gy. Therefore, a significant dose dependent early cell inactivation after single delivery of 16 Gy to 24 Gy to the cell monolayer was observed. With the increase of the post-irradiation incubation time, a better killing effect, as the consequence of clonogenic survival, was detected.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry",
title = "Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: I. survival of HTB140 melanoma cells",
volume = "1",
pages = "426-428",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9506"
}

Petrović, I. M., Todorović, D. V., Valastro, L. M., Cirrone, G. A. P., Cuttone, G.,& Ristić-Fira, A.. (2004). Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: I. survival of HTB140 melanoma cells. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
Society of Physical Chemists of Serbia., 1, 426-428.
https://hdl.handle.net/21.15107/rcub_vinar_9506

Petrović IM, Todorović DV, Valastro LM, Cirrone GAP, Cuttone G, Ristić-Fira A. Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: I. survival of HTB140 melanoma cells. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry. 2004;1:426-428.
https://hdl.handle.net/21.15107/rcub_vinar_9506 .

Petrović, Ivan M., Todorović, Danijela V., Valastro, Lucia M., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Ristić-Fira, Aleksandra, "Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: I. survival of HTB140 melanoma cells" in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry, 1 (2004):426-428,
https://hdl.handle.net/21.15107/rcub_vinar_9506 .

TY  - CONF
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Valastro, Lucia M.
AU  - Petrović, Ivan M.
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9507
AB  - The objective of this study was to determine whether apoptosis and cell cycle redistribution were influenced by high-LET irradiation. Exponentially growing HTB140 cells were exposed to an unmodulated 62 MeV proton beam, within the Bragg peak, delivered over the single dose range from 8 Gy to 24 Gy. At 6 h post-irradiation, there was a low level of early apoptosis. At 48 h irradiated cells were more damaged, showing the increase in number of apoptotic nuclei. The dose dependent cell cycle phase distribution was detected at 48 h post-irradiation. The cell population exhibited phase redistribution toward G2/M phase.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
T1  - Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: II. facs analyses of HTB140 melanoma cells
VL  - 1
SP  - 429
EP  - 431
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9507
ER  - 

@conference{
author = "Ristić-Fira, Aleksandra and Todorović, Danijela V. and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Valastro, Lucia M. and Petrović, Ivan M.",
year = "2004",
abstract = "The objective of this study was to determine whether apoptosis and cell cycle redistribution were influenced by high-LET irradiation. Exponentially growing HTB140 cells were exposed to an unmodulated 62 MeV proton beam, within the Bragg peak, delivered over the single dose range from 8 Gy to 24 Gy. At 6 h post-irradiation, there was a low level of early apoptosis. At 48 h irradiated cells were more damaged, showing the increase in number of apoptotic nuclei. The dose dependent cell cycle phase distribution was detected at 48 h post-irradiation. The cell population exhibited phase redistribution toward G2/M phase.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry",
title = "Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: II. facs analyses of HTB140 melanoma cells",
volume = "1",
pages = "429-431",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9507"
}

Ristić-Fira, A., Todorović, D. V., Cirrone, G. A. P., Cuttone, G., Valastro, L. M.,& Petrović, I. M.. (2004). Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: II. facs analyses of HTB140 melanoma cells. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
Society of Physical Chemists of Serbia., 1, 429-431.
https://hdl.handle.net/21.15107/rcub_vinar_9507

Ristić-Fira A, Todorović DV, Cirrone GAP, Cuttone G, Valastro LM, Petrović IM. Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: II. facs analyses of HTB140 melanoma cells. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry. 2004;1:429-431.
https://hdl.handle.net/21.15107/rcub_vinar_9507 .

Ristić-Fira, Aleksandra, Todorović, Danijela V., Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Valastro, Lucia M., Petrović, Ivan M., "Radiobiological studies on the 62 MeV therapeutic proton beam at lns catania: II. facs analyses of HTB140 melanoma cells" in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry, 1 (2004):429-431,
https://hdl.handle.net/21.15107/rcub_vinar_9507 .