TY  - JOUR
AU  - Dinčić, Marko
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Nešović Ostojić, Jelena
AU  - Kovačević, Sanjin
AU  - De Luka, Silvio R.
AU  - Đorđević, Drago M.
AU  - Ćirković, Saša
AU  - Brkić, Predrag
AU  - Todorović, Jasna
PY  - 2018
UR  - https://www.tandfonline.com/doi/full/10.1080/09553002.2018.1518611
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7957
AB  - Purpose: It is considered that exposure to static magnetic fields (SMF) may have both detrimental and therapeutic effect, but the mechanism of SMF influence on the living organisms is not well understood. Since the adenosine triphosphatases (ATPases) and acetylcholinesterase (AChE) are involved in both physiological and pathological processes, the modulation of Na+/K+-ATPase, ecto-ATPases and AChE activities, as well as oxidative stress responses were followed in synaptosomes isolated from rats after chronic exposure toward differently oriented SMF. Material and methods: Wistar albino rats were randomly divided into three experimental groups (six animals per group): Up and Down group - exposed to upward and downward oriented SMF, respectively, and Control group. After 50 days, the rats were sacrificed, and synaptosomes were isolated from the whole rat brain and used for testing the enzyme activities and oxidative stress parameters. Results: Chronic exposure to 1 mT SMF significantly increased ATPases, AChE activities, and malondialdehyde (MDA) level in both exposed groups, compared to control values. The significant decrease in synaptosomal catalase activity (1.48 ± 0.17 U/mg protein) induced by exposure to the downward oriented field, compared to those obtained for Control group (2.60 ± 0.29 U/mg protein), and Up group (2.72 ± 0.21 U/mg protein). Conclusions: It could be concluded that chronic exposure to differently oriented SMF increases ATPases and AChE activities in rat synaptosomes. Since brain ATPases and AChE have important roles in the pathogenesis of several neurological diseases, SMF influence on the activity of these enzymes may have potential therapeutic importance. © 2018, Copyright © 2018 Taylor & Francis Group, LLC.
T2  - International Journal of Radiation Biology
T1  - Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field
VL  - 94
IS  - 11
SP  - 1062
EP  - 1071
DO  - 10.1080/09553002.2018.1518611
ER  - 

@article{
author = "Dinčić, Marko and Krstić, Danijela Z. and Čolović, Mirjana B. and Nešović Ostojić, Jelena and Kovačević, Sanjin and De Luka, Silvio R. and Đorđević, Drago M. and Ćirković, Saša and Brkić, Predrag and Todorović, Jasna",
year = "2018",
abstract = "Purpose: It is considered that exposure to static magnetic fields (SMF) may have both detrimental and therapeutic effect, but the mechanism of SMF influence on the living organisms is not well understood. Since the adenosine triphosphatases (ATPases) and acetylcholinesterase (AChE) are involved in both physiological and pathological processes, the modulation of Na+/K+-ATPase, ecto-ATPases and AChE activities, as well as oxidative stress responses were followed in synaptosomes isolated from rats after chronic exposure toward differently oriented SMF. Material and methods: Wistar albino rats were randomly divided into three experimental groups (six animals per group): Up and Down group - exposed to upward and downward oriented SMF, respectively, and Control group. After 50 days, the rats were sacrificed, and synaptosomes were isolated from the whole rat brain and used for testing the enzyme activities and oxidative stress parameters. Results: Chronic exposure to 1 mT SMF significantly increased ATPases, AChE activities, and malondialdehyde (MDA) level in both exposed groups, compared to control values. The significant decrease in synaptosomal catalase activity (1.48 ± 0.17 U/mg protein) induced by exposure to the downward oriented field, compared to those obtained for Control group (2.60 ± 0.29 U/mg protein), and Up group (2.72 ± 0.21 U/mg protein). Conclusions: It could be concluded that chronic exposure to differently oriented SMF increases ATPases and AChE activities in rat synaptosomes. Since brain ATPases and AChE have important roles in the pathogenesis of several neurological diseases, SMF influence on the activity of these enzymes may have potential therapeutic importance. © 2018, Copyright © 2018 Taylor & Francis Group, LLC.",
journal = "International Journal of Radiation Biology",
title = "Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field",
volume = "94",
number = "11",
pages = "1062-1071",
doi = "10.1080/09553002.2018.1518611"
}

Dinčić, M., Krstić, D. Z., Čolović, M. B., Nešović Ostojić, J., Kovačević, S., De Luka, S. R., Đorđević, D. M., Ćirković, S., Brkić, P.,& Todorović, J.. (2018). Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field. in International Journal of Radiation Biology, 94(11), 1062-1071.
https://doi.org/10.1080/09553002.2018.1518611

Dinčić M, Krstić DZ, Čolović MB, Nešović Ostojić J, Kovačević S, De Luka SR, Đorđević DM, Ćirković S, Brkić P, Todorović J. Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field. in International Journal of Radiation Biology. 2018;94(11):1062-1071.
doi:10.1080/09553002.2018.1518611 .

Dinčić, Marko, Krstić, Danijela Z., Čolović, Mirjana B., Nešović Ostojić, Jelena, Kovačević, Sanjin, De Luka, Silvio R., Đorđević, Drago M., Ćirković, Saša, Brkić, Predrag, Todorović, Jasna, "Modulation of rat synaptosomal ATPases and acetylcholinesterase activities induced by chronic exposure to the static magnetic field" in International Journal of Radiation Biology, 94, no. 11 (2018):1062-1071,
https://doi.org/10.1080/09553002.2018.1518611 . .

TY  - JOUR
AU  - Avramović, Nataša
AU  - Dragutinovic, V
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Trbovic, A.
AU  - De Luka, Silvio R.
AU  - Milovanović, I
AU  - Popovic, T.
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5239
AB  - Background: The omega 3 fatty acids play an important role in many physiological processes. Their effect is well documented in neurodegenerative diseases and inflammatory diseases. Also, aging as a biophysiological process could be influenced by eicosapentanoic acid (EPA) and docosahexanoic acid (DHA) components of fish oil. However there are not many studies showing the effect of PUFA (polyunsaturated FA) suplementation in eldery brain functions and the response to oxidative strees. The aim of this study was to investigate the effects of dietary omega-3 fatty acid supplementation on levels of lipid peroxidation and oxidant/antioxidant status of brain tissue in aged (24 months old) Wistar rats. Methods: Animals were divided in two groups. Control group (n=8) was fed with standard laboratory food and received water ad libitum. Treated group (n=8) was also fed with standard laboratory food, water ad libitum and received fish oil capsules (EPA+DHA) for 6 weeks. Daily dose was 30mg EPA and 45mg DHA (capsules: 200mg EPA and 300mg DHA; in-house method). At the end of treatment animals were sacrificed and brains were collected and frozen on -80 degrees C. The levels of lipid peroxidation (malondialdehyde - MDA), activity of catalase (CAT) and activity of superoxide dismutase (SOD) were examined in cerebral cortex. Catalase activity was determined by measuring the decrease in absorbance (H2O2 degradation) at 240 nm for 3 min and expressed as U/mg protein. Total SOD (superoxide dismutase) activity was performed at room temperature according to the method of Misra and Fridovich. The extent of lipid peroxidation (LPO) was estimated as the concentration of thiobarbituric acid reactive product malondialdehyde (MDA) by using the method of Aruoma et al. The incorporation of fatty acids in cellular membranes was confirmed by gas chromatography. Results: Our results showed that lipid peroxidation significantly decreased in treated animal group, where MDA concentration was 0.38 +/- 0.001 vs. 0.43 +/- 0.001 nM/ml (p LT 0.05) in control. However SOD activity increased significantly in treated animal group 1.57 +/- 0.24 vs. 4.12 +/- 0.15 U/gHb/L (p LT 0.01) in control. CAT activity decreased in treated group but not significantly. Conclusion: Incorporation of omega-3 fatty acids after their supplementation had beneficial effects on brain tissue. Omega-3 fatty acids increased activity of SOD and decreased lipid peroxidation. Changes in oxidative/antioxidative balance are a result of EPA and DHA effects on lipids and enzymes of antioxidative system. Hippokratia 2012; 16 (3): 214-245
T2  - Hippokratia
T1  - The effects of omega 3 fatty acid supplementation on brain tissue oxidative status in aged wistar rats
VL  - 16
IS  - 3
SP  - 241
EP  - 245
UR  - https://hdl.handle.net/21.15107/rcub_vinar_5239
ER  - 

@article{
author = "Avramović, Nataša and Dragutinovic, V and Krstić, Danijela Z. and Čolović, Mirjana B. and Trbovic, A. and De Luka, Silvio R. and Milovanović, I and Popovic, T.",
year = "2012",
abstract = "Background: The omega 3 fatty acids play an important role in many physiological processes. Their effect is well documented in neurodegenerative diseases and inflammatory diseases. Also, aging as a biophysiological process could be influenced by eicosapentanoic acid (EPA) and docosahexanoic acid (DHA) components of fish oil. However there are not many studies showing the effect of PUFA (polyunsaturated FA) suplementation in eldery brain functions and the response to oxidative strees. The aim of this study was to investigate the effects of dietary omega-3 fatty acid supplementation on levels of lipid peroxidation and oxidant/antioxidant status of brain tissue in aged (24 months old) Wistar rats. Methods: Animals were divided in two groups. Control group (n=8) was fed with standard laboratory food and received water ad libitum. Treated group (n=8) was also fed with standard laboratory food, water ad libitum and received fish oil capsules (EPA+DHA) for 6 weeks. Daily dose was 30mg EPA and 45mg DHA (capsules: 200mg EPA and 300mg DHA; in-house method). At the end of treatment animals were sacrificed and brains were collected and frozen on -80 degrees C. The levels of lipid peroxidation (malondialdehyde - MDA), activity of catalase (CAT) and activity of superoxide dismutase (SOD) were examined in cerebral cortex. Catalase activity was determined by measuring the decrease in absorbance (H2O2 degradation) at 240 nm for 3 min and expressed as U/mg protein. Total SOD (superoxide dismutase) activity was performed at room temperature according to the method of Misra and Fridovich. The extent of lipid peroxidation (LPO) was estimated as the concentration of thiobarbituric acid reactive product malondialdehyde (MDA) by using the method of Aruoma et al. The incorporation of fatty acids in cellular membranes was confirmed by gas chromatography. Results: Our results showed that lipid peroxidation significantly decreased in treated animal group, where MDA concentration was 0.38 +/- 0.001 vs. 0.43 +/- 0.001 nM/ml (p LT 0.05) in control. However SOD activity increased significantly in treated animal group 1.57 +/- 0.24 vs. 4.12 +/- 0.15 U/gHb/L (p LT 0.01) in control. CAT activity decreased in treated group but not significantly. Conclusion: Incorporation of omega-3 fatty acids after their supplementation had beneficial effects on brain tissue. Omega-3 fatty acids increased activity of SOD and decreased lipid peroxidation. Changes in oxidative/antioxidative balance are a result of EPA and DHA effects on lipids and enzymes of antioxidative system. Hippokratia 2012; 16 (3): 214-245",
journal = "Hippokratia",
title = "The effects of omega 3 fatty acid supplementation on brain tissue oxidative status in aged wistar rats",
volume = "16",
number = "3",
pages = "241-245",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5239"
}

Avramović, N., Dragutinovic, V., Krstić, D. Z., Čolović, M. B., Trbovic, A., De Luka, S. R., Milovanović, I.,& Popovic, T.. (2012). The effects of omega 3 fatty acid supplementation on brain tissue oxidative status in aged wistar rats. in Hippokratia, 16(3), 241-245.
https://hdl.handle.net/21.15107/rcub_vinar_5239

Avramović N, Dragutinovic V, Krstić DZ, Čolović MB, Trbovic A, De Luka SR, Milovanović I, Popovic T. The effects of omega 3 fatty acid supplementation on brain tissue oxidative status in aged wistar rats. in Hippokratia. 2012;16(3):241-245.
https://hdl.handle.net/21.15107/rcub_vinar_5239 .

Avramović, Nataša, Dragutinovic, V, Krstić, Danijela Z., Čolović, Mirjana B., Trbovic, A., De Luka, Silvio R., Milovanović, I, Popovic, T., "The effects of omega 3 fatty acid supplementation on brain tissue oxidative status in aged wistar rats" in Hippokratia, 16, no. 3 (2012):241-245,
https://hdl.handle.net/21.15107/rcub_vinar_5239 .

TY  - JOUR
AU  - Djordjevich, Drago M.
AU  - De Luka, Silvio R.
AU  - Milovanović, Ivan D.
AU  - Janković, Saša
AU  - Stefanovic, Srdjan
AU  - Veskovic-Moracanin, Slavica
AU  - Cirkovic, Sasa
AU  - Ilić, Anđelija Z.
AU  - Ristić-Đurović, Jasna L.
AU  - Trbovich, Alexander M.
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4920
AB  - Static magnetic fields (SMFs) are time independent fields whose intensity can be spatially dependent. This study investigates influence of subchronic continuous exposure to upward and downward directed SMF on hematological parameters and spleen cellularity in mice. The experiment is performed on the Northern hemisphere; consequently, the vertical component of geomagnetic field is directed downward. Male, Swiss-Webster, 6 weeks old mice were exposed to the vertically declining SMF. Mice were divided in three groups and continuously exposed or not exposed for 28 days to the SMF characterized by the averaged field of 16 mT and averaged field gradient of 10 mT/cm. Differently oriented SMF did not alter hemoglobin and hematocrit content among the groups. However, the groups exposed to the upward and downward fields had statistically significant higher levels of serum transferrin compared to the control. Moreover, spleen cellularity in animals in the downward group was significantly higher compared to the upward and control group. In addition, spleen lymphocytes in both of the exposed groups were significantly higher than in the control group. In contrast, spleen granulocytes in the exposed groups were significantly lower than in the control group. Significant decrease was also observed in brain and liver iron content with concomitant increase of iron in serum and spleen in exposed animals. Subchronic continuous exposure to 16 mT SMF caused lymphocyte and granulocyte redistribution between spleen and blood. This distribution is typical for stress induced hematological changes. These results suggest that observed changes were not due to an unspecific stress response, but that they were rather caused by specific adaptation to subchronic SMF exposure. (C) 2012 Elsevier Inc. All rights reserved.
T2  - Ecotoxicology and Environmental Safety
T1  - Hematological parameters changes in mice subchronically exposed to static magnetic fields of different orientations
VL  - 81
SP  - 98
EP  - 105
DO  - 10.1016/j.ecoenv.2012.04.025
ER  - 

@article{
author = "Djordjevich, Drago M. and De Luka, Silvio R. and Milovanović, Ivan D. and Janković, Saša and Stefanovic, Srdjan and Veskovic-Moracanin, Slavica and Cirkovic, Sasa and Ilić, Anđelija Z. and Ristić-Đurović, Jasna L. and Trbovich, Alexander M.",
year = "2012",
abstract = "Static magnetic fields (SMFs) are time independent fields whose intensity can be spatially dependent. This study investigates influence of subchronic continuous exposure to upward and downward directed SMF on hematological parameters and spleen cellularity in mice. The experiment is performed on the Northern hemisphere; consequently, the vertical component of geomagnetic field is directed downward. Male, Swiss-Webster, 6 weeks old mice were exposed to the vertically declining SMF. Mice were divided in three groups and continuously exposed or not exposed for 28 days to the SMF characterized by the averaged field of 16 mT and averaged field gradient of 10 mT/cm. Differently oriented SMF did not alter hemoglobin and hematocrit content among the groups. However, the groups exposed to the upward and downward fields had statistically significant higher levels of serum transferrin compared to the control. Moreover, spleen cellularity in animals in the downward group was significantly higher compared to the upward and control group. In addition, spleen lymphocytes in both of the exposed groups were significantly higher than in the control group. In contrast, spleen granulocytes in the exposed groups were significantly lower than in the control group. Significant decrease was also observed in brain and liver iron content with concomitant increase of iron in serum and spleen in exposed animals. Subchronic continuous exposure to 16 mT SMF caused lymphocyte and granulocyte redistribution between spleen and blood. This distribution is typical for stress induced hematological changes. These results suggest that observed changes were not due to an unspecific stress response, but that they were rather caused by specific adaptation to subchronic SMF exposure. (C) 2012 Elsevier Inc. All rights reserved.",
journal = "Ecotoxicology and Environmental Safety",
title = "Hematological parameters changes in mice subchronically exposed to static magnetic fields of different orientations",
volume = "81",
pages = "98-105",
doi = "10.1016/j.ecoenv.2012.04.025"
}

Djordjevich, D. M., De Luka, S. R., Milovanović, I. D., Janković, S., Stefanovic, S., Veskovic-Moracanin, S., Cirkovic, S., Ilić, A. Z., Ristić-Đurović, J. L.,& Trbovich, A. M.. (2012). Hematological parameters changes in mice subchronically exposed to static magnetic fields of different orientations. in Ecotoxicology and Environmental Safety, 81, 98-105.
https://doi.org/10.1016/j.ecoenv.2012.04.025

Djordjevich DM, De Luka SR, Milovanović ID, Janković S, Stefanovic S, Veskovic-Moracanin S, Cirkovic S, Ilić AZ, Ristić-Đurović JL, Trbovich AM. Hematological parameters changes in mice subchronically exposed to static magnetic fields of different orientations. in Ecotoxicology and Environmental Safety. 2012;81:98-105.
doi:10.1016/j.ecoenv.2012.04.025 .

Djordjevich, Drago M., De Luka, Silvio R., Milovanović, Ivan D., Janković, Saša, Stefanovic, Srdjan, Veskovic-Moracanin, Slavica, Cirkovic, Sasa, Ilić, Anđelija Z., Ristić-Đurović, Jasna L., Trbovich, Alexander M., "Hematological parameters changes in mice subchronically exposed to static magnetic fields of different orientations" in Ecotoxicology and Environmental Safety, 81 (2012):98-105,
https://doi.org/10.1016/j.ecoenv.2012.04.025 . .