Petrovic, Biljana

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  • Petrovic, Biljana (7)
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Author's Bibliography

New dinuclear palladium(II) complexes: Studies of the nucleophilic substitution reactions, DNA/BSA interactions and cytotoxic activity

Cocic, Dugan; Jovanovic, Snezana; Nišavić, Marija; Baskic, Dejan; Todorović, Danijela V.; Popovic, Suzana; Bugarcic, Zivadin D.; Petrovic, Biljana

(2017)

TY  - JOUR
AU  - Cocic, Dugan
AU  - Jovanovic, Snezana
AU  - Nišavić, Marija
AU  - Baskic, Dejan
AU  - Todorović, Danijela V.
AU  - Popovic, Suzana
AU  - Bugarcic, Zivadin D.
AU  - Petrovic, Biljana
PY  - 2017
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1758
AB  - Six new dinuclear Pd(II) complexes, [{Pd(2,2-bipy)Cl}(2)(mu-pz)](ClO4)2 (Pd1), [{Pd(dach)Cl}(2)(mu-pz)](ClO4)(2) (Pd2), [{Pd(en)Cl}(2)(mu-pz)] (ClO4)(2) (Pd3), [{Pd(2,2-bipy)Cl}(2)(mu-4,4-bipy)](ClO4)(2) (Pd4), [{Pd(dach)Cl}(2)(mu-4,4-bipy)] (ClO4)(2) (Pd5) and [{Pd(en)Cl-2(mu-4,4-bipy)](ClO4)(2) (Pd6) (where 2,2-bipy = 2,2-bipyridyl, pz = pyrazine, dach = trans-(+/-)-1,2-diaminocyclohexane, en = ethylenediamine, 4,4-bipy = 4,4-bipyridyl) have been synthesized and characterized by elemental microanalysis, IR, H-1 NMR and MALDI-TOF mass spectrometry. The pK(a) values of corresponding diaqua complexes were determined by spectrophotometric pH titration. Substitution reactions with thiourea (Tu), L-methionine (L-Met), L-cysteine (L-Cys), L-histidine (L-His) and guanosine-5-monophosphate (5-GMP) were studied under the pseudo-first order conditions at pH 7.2. Reactions of Pdl with Tu, L-Met and L-Cys were followed by decomposition of complexes, while structures of dinuclear complexes were preserved during the substitution with nitrogen donors. Interactions with calf-thymus DNA (CT DNA) were followed by absorption spectroscopy and fluorescence quenching measurements. All complexes can bind to CT-DNA exhibiting high intrinsic binding constants (K-b = 10(4)-10(5) M-1). Competitive studies with ethidium bromide (EB) have shown that complexes can displace DNA-bound EB. High values of binding constants towards bovine serum albumin protein (BSA) indicate good binding affinity. Finally, all complexes showed moderate to high cytotoxic activity against HeLa (human cervical epithelial carcinoma cell lines) and MDA-MB-231 (human breast epithelial carcinoma cell lines) tumor cell lines inducing apoptotic type cell death, whereas normal fibroblasts were significantly less sensitive. The impact on cell cycle of these cells was distinctive, where Pd4, Pd5 and Pd6 showed the most prominent effect arresting MDA-MB-231 (human lung fibroblast cell lines) cell in G1/S phase of cell cycle.
T2  - Journal of Inorganic Biochemistry
T1  - New dinuclear palladium(II) complexes: Studies of the nucleophilic substitution reactions, DNA/BSA interactions and cytotoxic activity
VL  - 175
SP  - 67
EP  - 79
DO  - 10.1016/j.jinorgbio.2017.07.009
ER  - 
@article{
author = "Cocic, Dugan and Jovanovic, Snezana and Nišavić, Marija and Baskic, Dejan and Todorović, Danijela V. and Popovic, Suzana and Bugarcic, Zivadin D. and Petrovic, Biljana",
year = "2017",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1758",
abstract = "Six new dinuclear Pd(II) complexes, [{Pd(2,2-bipy)Cl}(2)(mu-pz)](ClO4)2 (Pd1), [{Pd(dach)Cl}(2)(mu-pz)](ClO4)(2) (Pd2), [{Pd(en)Cl}(2)(mu-pz)] (ClO4)(2) (Pd3), [{Pd(2,2-bipy)Cl}(2)(mu-4,4-bipy)](ClO4)(2) (Pd4), [{Pd(dach)Cl}(2)(mu-4,4-bipy)] (ClO4)(2) (Pd5) and [{Pd(en)Cl-2(mu-4,4-bipy)](ClO4)(2) (Pd6) (where 2,2-bipy = 2,2-bipyridyl, pz = pyrazine, dach = trans-(+/-)-1,2-diaminocyclohexane, en = ethylenediamine, 4,4-bipy = 4,4-bipyridyl) have been synthesized and characterized by elemental microanalysis, IR, H-1 NMR and MALDI-TOF mass spectrometry. The pK(a) values of corresponding diaqua complexes were determined by spectrophotometric pH titration. Substitution reactions with thiourea (Tu), L-methionine (L-Met), L-cysteine (L-Cys), L-histidine (L-His) and guanosine-5-monophosphate (5-GMP) were studied under the pseudo-first order conditions at pH 7.2. Reactions of Pdl with Tu, L-Met and L-Cys were followed by decomposition of complexes, while structures of dinuclear complexes were preserved during the substitution with nitrogen donors. Interactions with calf-thymus DNA (CT DNA) were followed by absorption spectroscopy and fluorescence quenching measurements. All complexes can bind to CT-DNA exhibiting high intrinsic binding constants (K-b = 10(4)-10(5) M-1). Competitive studies with ethidium bromide (EB) have shown that complexes can displace DNA-bound EB. High values of binding constants towards bovine serum albumin protein (BSA) indicate good binding affinity. Finally, all complexes showed moderate to high cytotoxic activity against HeLa (human cervical epithelial carcinoma cell lines) and MDA-MB-231 (human breast epithelial carcinoma cell lines) tumor cell lines inducing apoptotic type cell death, whereas normal fibroblasts were significantly less sensitive. The impact on cell cycle of these cells was distinctive, where Pd4, Pd5 and Pd6 showed the most prominent effect arresting MDA-MB-231 (human lung fibroblast cell lines) cell in G1/S phase of cell cycle.",
journal = "Journal of Inorganic Biochemistry",
title = "New dinuclear palladium(II) complexes: Studies of the nucleophilic substitution reactions, DNA/BSA interactions and cytotoxic activity",
volume = "175",
pages = "67-79",
doi = "10.1016/j.jinorgbio.2017.07.009"
}
Cocic, D., Jovanovic, S., Nišavić, M., Baskic, D., Todorović, D. V., Popovic, S., Bugarcic, Z. D.,& Petrovic, B. (2017). New dinuclear palladium(II) complexes: Studies of the nucleophilic substitution reactions, DNA/BSA interactions and cytotoxic activity.
Journal of Inorganic Biochemistry, 175, 67-79.
https://doi.org/10.1016/j.jinorgbio.2017.07.009
Cocic D, Jovanovic S, Nišavić M, Baskic D, Todorović DV, Popovic S, Bugarcic ZD, Petrovic B. New dinuclear palladium(II) complexes: Studies of the nucleophilic substitution reactions, DNA/BSA interactions and cytotoxic activity. Journal of Inorganic Biochemistry. 2017;175:67-79
Cocic Dugan, Jovanovic Snezana, Nišavić Marija, Baskic Dejan, Todorović Danijela V., Popovic Suzana, Bugarcic Zivadin D., Petrovic Biljana, "New dinuclear palladium(II) complexes: Studies of the nucleophilic substitution reactions, DNA/BSA interactions and cytotoxic activity" Journal of Inorganic Biochemistry, 175 (2017):67-79,
https://doi.org/10.1016/j.jinorgbio.2017.07.009 .
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New bimetallic palladium(II) and platinum(II) complexes: studies of the nucleophilic substitution reactions, interactions with CT-DNA, bovine serum albumin and cytotoxic activity

Jovanovic, Snezana; Obrencevic, Katarina; Bugarcic, Zivadin D.; Popović, Iva A.; Žakula, Jelena; Petrovic, Biljana

(2016)

TY  - JOUR
AU  - Jovanovic, Snezana
AU  - Obrencevic, Katarina
AU  - Bugarcic, Zivadin D.
AU  - Popović, Iva A.
AU  - Žakula, Jelena
AU  - Petrovic, Biljana
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1208
AB  - Two new dinuclear bimetallic complexes, [{PdCl(bipy)}{mu-(pyrazine)}{PtCl(bipy)}]Cl(ClO4) (1) (bipy is 2,2-=bipyridine) and [{PdCl(en)}{mu-(pyrazine)}{PtCl(en)}]Cl(ClO4) (2) (en is ethylenediamine), have been synthesized and characterized by elemental microanalysis, IR, H-1 NMR spectroscopy and MALDI-TOF mass spectrometry. The pK(a) values of the coordinated water molecules of the diaqua species were determined as well. Substitution reactions of complexes (1) and (2) with thiourea (Tu), L-methionine (L-Met), L-cysteine (L-Cys), L-histidine (L-His) and guanosine-5-monophosphate (5-GMP) were studied under the pseudo-first order conditions as a function of nucleophile concentration and temperature. The order of reactivity of nucleophiles was: Tu GT L-Met GT L-Cys GT L-His GT 5-GMP. Substitution reactions with Tu, L-Cys and L-His were followed by decomposition of bimetallic complexes to the corresponding substituted mononuclear complexes [Pd(N-N)(Nu)(2)] and [Pt(N-N)(Nu)(2)] (N-N = bipy, en), releasing the bridging ligand. However, the structures of starting bimetallic complexes were preserved during the reactions with L-Met and 5-GMP. The absorption spectroscopic study of interactions of calf-thymus DNA (CT-DNA) with complexes (1), (2) and [{PdCl(bipy)}{mu-(NH2(CH2)(6)H2N)} {PtCl(bipy)}] Cl(ClO4) (3), has shown that all the complexes exhibit high intrinsic binding constants (K-b = 10(4)-10(5) M-1). DNA-ethidium bromide (DNA-EB) fluorescence was quenched after addition of complexes (1), (2) or (3), indicating displacement of intercalating EB by complexes. All complexes have shown good binding affinity to bovine serum albumin protein (BSA). Chemosensitivity of A375 (human melanoma) and HeLa (human cervical cancer) cell lines toward complexes (1), (2) and (3) was analyzed by SRB assay. Complex (1) displayed significant inhibitory effect on the growth of both cell lines.
T2  - Dalton Transactions
T1  - New bimetallic palladium(II) and platinum(II) complexes: studies of the nucleophilic substitution reactions, interactions with CT-DNA, bovine serum albumin and cytotoxic activity
VL  - 45
IS  - 31
SP  - 12444
EP  - 12457
DO  - 10.1039/c6dt02226j
ER  - 
@article{
author = "Jovanovic, Snezana and Obrencevic, Katarina and Bugarcic, Zivadin D. and Popović, Iva A. and Žakula, Jelena and Petrovic, Biljana",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1208",
abstract = "Two new dinuclear bimetallic complexes, [{PdCl(bipy)}{mu-(pyrazine)}{PtCl(bipy)}]Cl(ClO4) (1) (bipy is 2,2-=bipyridine) and [{PdCl(en)}{mu-(pyrazine)}{PtCl(en)}]Cl(ClO4) (2) (en is ethylenediamine), have been synthesized and characterized by elemental microanalysis, IR, H-1 NMR spectroscopy and MALDI-TOF mass spectrometry. The pK(a) values of the coordinated water molecules of the diaqua species were determined as well. Substitution reactions of complexes (1) and (2) with thiourea (Tu), L-methionine (L-Met), L-cysteine (L-Cys), L-histidine (L-His) and guanosine-5-monophosphate (5-GMP) were studied under the pseudo-first order conditions as a function of nucleophile concentration and temperature. The order of reactivity of nucleophiles was: Tu GT L-Met GT L-Cys GT L-His GT 5-GMP. Substitution reactions with Tu, L-Cys and L-His were followed by decomposition of bimetallic complexes to the corresponding substituted mononuclear complexes [Pd(N-N)(Nu)(2)] and [Pt(N-N)(Nu)(2)] (N-N = bipy, en), releasing the bridging ligand. However, the structures of starting bimetallic complexes were preserved during the reactions with L-Met and 5-GMP. The absorption spectroscopic study of interactions of calf-thymus DNA (CT-DNA) with complexes (1), (2) and [{PdCl(bipy)}{mu-(NH2(CH2)(6)H2N)} {PtCl(bipy)}] Cl(ClO4) (3), has shown that all the complexes exhibit high intrinsic binding constants (K-b = 10(4)-10(5) M-1). DNA-ethidium bromide (DNA-EB) fluorescence was quenched after addition of complexes (1), (2) or (3), indicating displacement of intercalating EB by complexes. All complexes have shown good binding affinity to bovine serum albumin protein (BSA). Chemosensitivity of A375 (human melanoma) and HeLa (human cervical cancer) cell lines toward complexes (1), (2) and (3) was analyzed by SRB assay. Complex (1) displayed significant inhibitory effect on the growth of both cell lines.",
journal = "Dalton Transactions",
title = "New bimetallic palladium(II) and platinum(II) complexes: studies of the nucleophilic substitution reactions, interactions with CT-DNA, bovine serum albumin and cytotoxic activity",
volume = "45",
number = "31",
pages = "12444-12457",
doi = "10.1039/c6dt02226j"
}
Jovanovic, S., Obrencevic, K., Bugarcic, Z. D., Popović, I. A., Žakula, J.,& Petrovic, B. (2016). New bimetallic palladium(II) and platinum(II) complexes: studies of the nucleophilic substitution reactions, interactions with CT-DNA, bovine serum albumin and cytotoxic activity.
Dalton Transactions, 45(31), 12444-12457.
https://doi.org/10.1039/c6dt02226j
Jovanovic S, Obrencevic K, Bugarcic ZD, Popović IA, Žakula J, Petrovic B. New bimetallic palladium(II) and platinum(II) complexes: studies of the nucleophilic substitution reactions, interactions with CT-DNA, bovine serum albumin and cytotoxic activity. Dalton Transactions. 2016;45(31):12444-12457
Jovanovic Snezana, Obrencevic Katarina, Bugarcic Zivadin D., Popović Iva A., Žakula Jelena, Petrovic Biljana, "New bimetallic palladium(II) and platinum(II) complexes: studies of the nucleophilic substitution reactions, interactions with CT-DNA, bovine serum albumin and cytotoxic activity" Dalton Transactions, 45, no. 31 (2016):12444-12457,
https://doi.org/10.1039/c6dt02226j .
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Kinetics and mechanism of the substitution reactions of some monofunctional Pt(II) complexes with heterocyclic nitrogen donor molecules. Crystal structure of [Pt(bpma)(pzBr)]Cl-2 center dot 2H(2)O

Kosovic, Milica; Jovanovic, Snezana; Bogdanović, Goran A.; Giester, Gerald; Jacimovic, Zeljko; Bugarcic, Zivadin D.; Petrovic, Biljana

(2016)

TY  - JOUR
AU  - Kosovic, Milica
AU  - Jovanovic, Snezana
AU  - Bogdanović, Goran A.
AU  - Giester, Gerald
AU  - Jacimovic, Zeljko
AU  - Bugarcic, Zivadin D.
AU  - Petrovic, Biljana
PY  - 2016
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/1253
AB  - Substitution reactions of [Pt(terpy)Cl](+) (terpy=2,2;6,2-terpyridine), [Pt(bpma)Cl](+) (bpma=bis(2-pyridylmethyl)amine), [Pt(dien)Cl](+) (dien=diethylenetriamine or 1,5-diamino-3-azapentane) and [Pt(tpdm)Cl](+) (tpdm=tripyridinedimethane) with nitrogen donor heterocyclic molecules, such as 3-amino-4-iodo-pyrazole (pzI), 5-amino-4-bromo-3-methyl-pyrazole (pzBr) and imidazole (Im), were studied in aqueous 0.10M NaClO4 in the presence of 10mM NaCl using variable-temperature UV-vis spectrophotometry. The second-order rate constants k(2) indicate decrease in reactivity in the order [Pt(terpy)Cl](+) GT [Pt(bpma)Cl](+) GT [Pt(tpdm)Cl](+) GT [Pt(dien)Cl](+). The most reactive nucleophile among the heterocyclic compounds is imidazole, while pzI shows slightly higher reactivity than pzBr. Activation parameters were also determined and the negative values for entropies of activation, S, support an associative mode of substitution for all substitution processes. Crystal structure of [Pt(bpma)(pzBr)]Cl(2)2H(2)O was determined by single-crystal X-ray analysis. The coordination geometry of the complex is distorted square-planar while the bond distance Pt-N2(pzBr) is longer than the other three Pt-N distances.
T2  - Journal of Coordination Chemistry
T1  - Kinetics and mechanism of the substitution reactions of some monofunctional Pt(II) complexes with heterocyclic nitrogen donor molecules. Crystal structure of [Pt(bpma)(pzBr)]Cl-2 center dot 2H(2)O
VL  - 69
IS  - 19
SP  - 2819
EP  - 2831
DO  - 10.1080/00958972.2016.1224336
ER  - 
@article{
author = "Kosovic, Milica and Jovanovic, Snezana and Bogdanović, Goran A. and Giester, Gerald and Jacimovic, Zeljko and Bugarcic, Zivadin D. and Petrovic, Biljana",
year = "2016",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/1253",
abstract = "Substitution reactions of [Pt(terpy)Cl](+) (terpy=2,2;6,2-terpyridine), [Pt(bpma)Cl](+) (bpma=bis(2-pyridylmethyl)amine), [Pt(dien)Cl](+) (dien=diethylenetriamine or 1,5-diamino-3-azapentane) and [Pt(tpdm)Cl](+) (tpdm=tripyridinedimethane) with nitrogen donor heterocyclic molecules, such as 3-amino-4-iodo-pyrazole (pzI), 5-amino-4-bromo-3-methyl-pyrazole (pzBr) and imidazole (Im), were studied in aqueous 0.10M NaClO4 in the presence of 10mM NaCl using variable-temperature UV-vis spectrophotometry. The second-order rate constants k(2) indicate decrease in reactivity in the order [Pt(terpy)Cl](+) GT [Pt(bpma)Cl](+) GT [Pt(tpdm)Cl](+) GT [Pt(dien)Cl](+). The most reactive nucleophile among the heterocyclic compounds is imidazole, while pzI shows slightly higher reactivity than pzBr. Activation parameters were also determined and the negative values for entropies of activation, S, support an associative mode of substitution for all substitution processes. Crystal structure of [Pt(bpma)(pzBr)]Cl(2)2H(2)O was determined by single-crystal X-ray analysis. The coordination geometry of the complex is distorted square-planar while the bond distance Pt-N2(pzBr) is longer than the other three Pt-N distances.",
journal = "Journal of Coordination Chemistry",
title = "Kinetics and mechanism of the substitution reactions of some monofunctional Pt(II) complexes with heterocyclic nitrogen donor molecules. Crystal structure of [Pt(bpma)(pzBr)]Cl-2 center dot 2H(2)O",
volume = "69",
number = "19",
pages = "2819-2831",
doi = "10.1080/00958972.2016.1224336"
}
Kosovic, M., Jovanovic, S., Bogdanović, G. A., Giester, G., Jacimovic, Z., Bugarcic, Z. D.,& Petrovic, B. (2016). Kinetics and mechanism of the substitution reactions of some monofunctional Pt(II) complexes with heterocyclic nitrogen donor molecules. Crystal structure of [Pt(bpma)(pzBr)]Cl-2 center dot 2H(2)O.
Journal of Coordination Chemistry, 69(19), 2819-2831.
https://doi.org/10.1080/00958972.2016.1224336
Kosovic M, Jovanovic S, Bogdanović GA, Giester G, Jacimovic Z, Bugarcic ZD, Petrovic B. Kinetics and mechanism of the substitution reactions of some monofunctional Pt(II) complexes with heterocyclic nitrogen donor molecules. Crystal structure of [Pt(bpma)(pzBr)]Cl-2 center dot 2H(2)O. Journal of Coordination Chemistry. 2016;69(19):2819-2831
Kosovic Milica, Jovanovic Snezana, Bogdanović Goran A., Giester Gerald, Jacimovic Zeljko, Bugarcic Zivadin D., Petrovic Biljana, "Kinetics and mechanism of the substitution reactions of some monofunctional Pt(II) complexes with heterocyclic nitrogen donor molecules. Crystal structure of [Pt(bpma)(pzBr)]Cl-2 center dot 2H(2)O" Journal of Coordination Chemistry, 69, no. 19 (2016):2819-2831,
https://doi.org/10.1080/00958972.2016.1224336 .
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Kinetics and mechanism of substitution reactions of the new bimetallic [{PdCl(bipy)}{mu-(NH2(CH2)(6)H2N)}{PtCl(biPY)}]Cl(ClO4) complex with important bio-molecules

Jovanovic, Snezana; Petrovic, Biljana; Petković, Marijana; Bugarcic, Zivadin D.

(2015)

TY  - JOUR
AU  - Jovanovic, Snezana
AU  - Petrovic, Biljana
AU  - Petković, Marijana
AU  - Bugarcic, Zivadin D.
PY  - 2015
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/841
AB  - The new dinuclear bimetallic complex, [{PdCl(bipy)}{mu-(NH2(CH2)(6)H2N)}{PtCl(biPY)}]Cl(ClO4) (bipy is 2,2-bipyridine), has been prepared and characterized by elemental microanalysis, IR, H-1 NMR spectroscopy and MALDI-TOF mass spectrometry. Substitution reactions of the studied complex with selected biologically important ligands such as: thiourea (Tu), L-methionine (L-Met), L-cysteine (L-Cys), L-histidine (L-His) and guanosine-5-monophosphate (5-GMP), were studied under the pseudo-first order conditions as a function of concentration and temperature using stopped-flow and UV-Vis spectrophotometry. The reactions were monitored in 0.1 M NaClO4 at pH 5.0, in the presence of 40 mM NaCI. All fast reactions were monitored by stopped-flow at three temperatures (288 K, 298 K, 308 K) to determine the activation parameters, while the reactions studied by UV-Vis spectrophotometry were tested only at 298 K. Observed order of reactivity of the used ligands is: Tu GT L-Met GT L-Cys GT L-His GT 5-GMP. Substitution reactions of the investigated bimetallic complex with Tu, L-Cys and L-His were followed by degradation to the corresponding substituted mononuclear complexes of palladium (II) and platinum (II), [Pd(bipy)(Nu)(2)] and [Pt(bipy)(Nu)(2)] (where Nu = Tu, L-Cys, L-His), by releasing of the bridge ligand,1,6-diaminohexane. In contrast, during the substitution reactions with L-Met and 5-GMP, the structure of starting bimetallic complex was preserved and the process of degradation can be halted. The proposed pathways of the substitution reactions of [{PdCl(bipy)}{mu-(NH2(CH2)(6)H2N)}{PtCl(biPY)}]Cl(ClO4) complex with all selected ligands were confirmed by H-1 NMR spectroscopy at 295 K. Additionally, the two pK(a) values of studied diaqua complex, [(Pd(H2O)(bipy)}(mu-(NH2(CH2)(6)H2N)}(Pt(H2O)(biPY)}](4+), were determined by spectrophotometric pH titration. The large negative values for the entropy of activation, Delta S-not equal, support an associative substitution mechanism. (C) 2015 Elsevier Ltd. All rights reserved.
T2  - Polyhedron
T1  - Kinetics and mechanism of substitution reactions of the new bimetallic [{PdCl(bipy)}{mu-(NH2(CH2)(6)H2N)}{PtCl(biPY)}]Cl(ClO4) complex with important bio-molecules
VL  - 101
SP  - 206
EP  - 214
DO  - 10.1016/j.poly.2015.09.021
ER  - 
@article{
author = "Jovanovic, Snezana and Petrovic, Biljana and Petković, Marijana and Bugarcic, Zivadin D.",
year = "2015",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/841",
abstract = "The new dinuclear bimetallic complex, [{PdCl(bipy)}{mu-(NH2(CH2)(6)H2N)}{PtCl(biPY)}]Cl(ClO4) (bipy is 2,2-bipyridine), has been prepared and characterized by elemental microanalysis, IR, H-1 NMR spectroscopy and MALDI-TOF mass spectrometry. Substitution reactions of the studied complex with selected biologically important ligands such as: thiourea (Tu), L-methionine (L-Met), L-cysteine (L-Cys), L-histidine (L-His) and guanosine-5-monophosphate (5-GMP), were studied under the pseudo-first order conditions as a function of concentration and temperature using stopped-flow and UV-Vis spectrophotometry. The reactions were monitored in 0.1 M NaClO4 at pH 5.0, in the presence of 40 mM NaCI. All fast reactions were monitored by stopped-flow at three temperatures (288 K, 298 K, 308 K) to determine the activation parameters, while the reactions studied by UV-Vis spectrophotometry were tested only at 298 K. Observed order of reactivity of the used ligands is: Tu GT L-Met GT L-Cys GT L-His GT 5-GMP. Substitution reactions of the investigated bimetallic complex with Tu, L-Cys and L-His were followed by degradation to the corresponding substituted mononuclear complexes of palladium (II) and platinum (II), [Pd(bipy)(Nu)(2)] and [Pt(bipy)(Nu)(2)] (where Nu = Tu, L-Cys, L-His), by releasing of the bridge ligand,1,6-diaminohexane. In contrast, during the substitution reactions with L-Met and 5-GMP, the structure of starting bimetallic complex was preserved and the process of degradation can be halted. The proposed pathways of the substitution reactions of [{PdCl(bipy)}{mu-(NH2(CH2)(6)H2N)}{PtCl(biPY)}]Cl(ClO4) complex with all selected ligands were confirmed by H-1 NMR spectroscopy at 295 K. Additionally, the two pK(a) values of studied diaqua complex, [(Pd(H2O)(bipy)}(mu-(NH2(CH2)(6)H2N)}(Pt(H2O)(biPY)}](4+), were determined by spectrophotometric pH titration. The large negative values for the entropy of activation, Delta S-not equal, support an associative substitution mechanism. (C) 2015 Elsevier Ltd. All rights reserved.",
journal = "Polyhedron",
title = "Kinetics and mechanism of substitution reactions of the new bimetallic [{PdCl(bipy)}{mu-(NH2(CH2)(6)H2N)}{PtCl(biPY)}]Cl(ClO4) complex with important bio-molecules",
volume = "101",
pages = "206-214",
doi = "10.1016/j.poly.2015.09.021"
}
Jovanovic, S., Petrovic, B., Petković, M.,& Bugarcic, Z. D. (2015). Kinetics and mechanism of substitution reactions of the new bimetallic [{PdCl(bipy)}{mu-(NH2(CH2)(6)H2N)}{PtCl(biPY)}]Cl(ClO4) complex with important bio-molecules.
Polyhedron, 101, 206-214.
https://doi.org/10.1016/j.poly.2015.09.021
Jovanovic S, Petrovic B, Petković M, Bugarcic ZD. Kinetics and mechanism of substitution reactions of the new bimetallic [{PdCl(bipy)}{mu-(NH2(CH2)(6)H2N)}{PtCl(biPY)}]Cl(ClO4) complex with important bio-molecules. Polyhedron. 2015;101:206-214
Jovanovic Snezana, Petrovic Biljana, Petković Marijana, Bugarcic Zivadin D., "Kinetics and mechanism of substitution reactions of the new bimetallic [{PdCl(bipy)}{mu-(NH2(CH2)(6)H2N)}{PtCl(biPY)}]Cl(ClO4) complex with important bio-molecules" Polyhedron, 101 (2015):206-214,
https://doi.org/10.1016/j.poly.2015.09.021 .
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Laser desorption and ionization time-of-flight versus matrix-assisted laser desorption and ionization time-of-flight mass spectrometry of Pt(II) and Ru(III) metal complexes

Damnjanovic, Bojana; Kamceva, Tina; Petrovic, Biljana; Bugarcic, Zivadin D.; Petković, Marijana

(2011)

TY  - JOUR
AU  - Damnjanovic, Bojana
AU  - Kamceva, Tina
AU  - Petrovic, Biljana
AU  - Bugarcic, Zivadin D.
AU  - Petković, Marijana
PY  - 2011
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4237
AB  - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been recently established as a powerful, soft ionization technique for the analysis of both transition metal complexes, which are used as metallo-drugs in the therapy of various types of tumors, and biomolecules. Since some metal complexes absorb light in the UV range, it should be possible to analyse them without additional matrices, i.e. using LDI-TOF MS. In this study, the matrix-free approach was tested for the analysis of [PtCl(2)(dach)] (dichloride(1,2-diamincyclohexane) platinum(II)), [RuCl(2)(en)(2)]Cl (dichloridobis(ethylenediamine) ruthenium(III) chloride) and [RuCl(2)(bipy)(2)]Cl (bis(bipyridine)dichloridoruthenium(III) chloride) and the detection limit for these compounds was determined. In summary, the LDI-TOF mass spectra of [PtCl(2)(dach)] and [RuCl(2)(en)(2)]Cl are rather simple, whereas in the presence of 2,5-DHB as a matrix, additional peaks are generated. On the other hand, the standard MALDI-TOF mass spectrum of [RuCl(2)(bipy)(2)]Cl exhibits only one peak arising from the complex, in contrast to six peaks detectable in the LDI-TOF mass spectrum. The detection limit in the MALDI-TOF MS analysis of [PtCl(2)(dach)] and [RuCl(2)(bipy)(2)]Cl complexes was lower than that determined in LDI-TOF MS. Taking all into account, in this paper, we have demonstrated some advantages and drawbacks of the matrix-free LDI-TOF mass spectrometric analysis of transition metal complexes.
T2  - Analytical Methods
T1  - Laser desorption and ionization time-of-flight versus matrix-assisted laser desorption and ionization time-of-flight mass spectrometry of Pt(II) and Ru(III) metal complexes
VL  - 3
IS  - 2
SP  - 400
EP  - 407
DO  - 10.1039/c0ay00517g
ER  - 
@article{
author = "Damnjanovic, Bojana and Kamceva, Tina and Petrovic, Biljana and Bugarcic, Zivadin D. and Petković, Marijana",
year = "2011",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/4237",
abstract = "Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been recently established as a powerful, soft ionization technique for the analysis of both transition metal complexes, which are used as metallo-drugs in the therapy of various types of tumors, and biomolecules. Since some metal complexes absorb light in the UV range, it should be possible to analyse them without additional matrices, i.e. using LDI-TOF MS. In this study, the matrix-free approach was tested for the analysis of [PtCl(2)(dach)] (dichloride(1,2-diamincyclohexane) platinum(II)), [RuCl(2)(en)(2)]Cl (dichloridobis(ethylenediamine) ruthenium(III) chloride) and [RuCl(2)(bipy)(2)]Cl (bis(bipyridine)dichloridoruthenium(III) chloride) and the detection limit for these compounds was determined. In summary, the LDI-TOF mass spectra of [PtCl(2)(dach)] and [RuCl(2)(en)(2)]Cl are rather simple, whereas in the presence of 2,5-DHB as a matrix, additional peaks are generated. On the other hand, the standard MALDI-TOF mass spectrum of [RuCl(2)(bipy)(2)]Cl exhibits only one peak arising from the complex, in contrast to six peaks detectable in the LDI-TOF mass spectrum. The detection limit in the MALDI-TOF MS analysis of [PtCl(2)(dach)] and [RuCl(2)(bipy)(2)]Cl complexes was lower than that determined in LDI-TOF MS. Taking all into account, in this paper, we have demonstrated some advantages and drawbacks of the matrix-free LDI-TOF mass spectrometric analysis of transition metal complexes.",
journal = "Analytical Methods",
title = "Laser desorption and ionization time-of-flight versus matrix-assisted laser desorption and ionization time-of-flight mass spectrometry of Pt(II) and Ru(III) metal complexes",
volume = "3",
number = "2",
pages = "400-407",
doi = "10.1039/c0ay00517g"
}
Damnjanovic, B., Kamceva, T., Petrovic, B., Bugarcic, Z. D.,& Petković, M. (2011). Laser desorption and ionization time-of-flight versus matrix-assisted laser desorption and ionization time-of-flight mass spectrometry of Pt(II) and Ru(III) metal complexes.
Analytical Methods, 3(2), 400-407.
https://doi.org/10.1039/c0ay00517g
Damnjanovic B, Kamceva T, Petrovic B, Bugarcic ZD, Petković M. Laser desorption and ionization time-of-flight versus matrix-assisted laser desorption and ionization time-of-flight mass spectrometry of Pt(II) and Ru(III) metal complexes. Analytical Methods. 2011;3(2):400-407
Damnjanovic Bojana, Kamceva Tina, Petrovic Biljana, Bugarcic Zivadin D., Petković Marijana, "Laser desorption and ionization time-of-flight versus matrix-assisted laser desorption and ionization time-of-flight mass spectrometry of Pt(II) and Ru(III) metal complexes" Analytical Methods, 3, no. 2 (2011):400-407,
https://doi.org/10.1039/c0ay00517g .
12
13
12

Comparison of MALDI-TOF mass spectra of [PdCl(dien)]Cl and [Ru(en)(2)Cl-2]Cl acquired with different matrices

Damnjanovic, Bojana; Petrovic, Biljana; Dimitric-Markovic, Jasmina; Petković, Marijana

(2011)

TY  - JOUR
AU  - Damnjanovic, Bojana
AU  - Petrovic, Biljana
AU  - Dimitric-Markovic, Jasmina
AU  - Petković, Marijana
PY  - 2011
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/4637
AB  - In this work, the matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass spectra of two cationic complexes, i.e., [PdCl(dien)]Cl and [Ru(en)(7)Cl-2]Cl, acquired under different conditions were analyzed. The spectra were recorded with three matrices with or without trifluoroacetic acid (TFA), i.e., two traditional matrices, i.e., 2,5-dihydroxybenzoic acid and alpha-cyano-hydroxycinnamic acid, and one flavonoid, quercetin. The spectra acquired with quercetin appeared to be the simplest, whereas in the spectra obtained with other matrices, peaks arising either from the addition of matrix molecules or from the fragmentation products were detectable. Addition of TFA did not complicate the spectra of the Pd(II) and Ru(III) complexes when the traditional matrices were used. On the other hand, the spectra of Pd complex were simpler, whereas the addition of TFA in the case of the Ru complex resulted in a higher number of peaks, some of which could not be identified. Taken together, the results of this study once more emphasize the differences arising in the MALDI-TOF mass spectra of transition metal complexes in dependence on the applied matrix.
T2  - Journal of the Serbian Chemical Society
T1  - Comparison of MALDI-TOF mass spectra of [PdCl(dien)]Cl and [Ru(en)(2)Cl-2]Cl acquired with different matrices
VL  - 76
IS  - 12
SP  - 1687
EP  - 1701
DO  - 10.2298/JSC110201145D
ER  - 
@article{
author = "Damnjanovic, Bojana and Petrovic, Biljana and Dimitric-Markovic, Jasmina and Petković, Marijana",
year = "2011",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/4637",
abstract = "In this work, the matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass spectra of two cationic complexes, i.e., [PdCl(dien)]Cl and [Ru(en)(7)Cl-2]Cl, acquired under different conditions were analyzed. The spectra were recorded with three matrices with or without trifluoroacetic acid (TFA), i.e., two traditional matrices, i.e., 2,5-dihydroxybenzoic acid and alpha-cyano-hydroxycinnamic acid, and one flavonoid, quercetin. The spectra acquired with quercetin appeared to be the simplest, whereas in the spectra obtained with other matrices, peaks arising either from the addition of matrix molecules or from the fragmentation products were detectable. Addition of TFA did not complicate the spectra of the Pd(II) and Ru(III) complexes when the traditional matrices were used. On the other hand, the spectra of Pd complex were simpler, whereas the addition of TFA in the case of the Ru complex resulted in a higher number of peaks, some of which could not be identified. Taken together, the results of this study once more emphasize the differences arising in the MALDI-TOF mass spectra of transition metal complexes in dependence on the applied matrix.",
journal = "Journal of the Serbian Chemical Society",
title = "Comparison of MALDI-TOF mass spectra of [PdCl(dien)]Cl and [Ru(en)(2)Cl-2]Cl acquired with different matrices",
volume = "76",
number = "12",
pages = "1687-1701",
doi = "10.2298/JSC110201145D"
}
Damnjanovic, B., Petrovic, B., Dimitric-Markovic, J.,& Petković, M. (2011). Comparison of MALDI-TOF mass spectra of [PdCl(dien)]Cl and [Ru(en)(2)Cl-2]Cl acquired with different matrices.
Journal of the Serbian Chemical Society, 76(12), 1687-1701.
https://doi.org/10.2298/JSC110201145D
Damnjanovic B, Petrovic B, Dimitric-Markovic J, Petković M. Comparison of MALDI-TOF mass spectra of [PdCl(dien)]Cl and [Ru(en)(2)Cl-2]Cl acquired with different matrices. Journal of the Serbian Chemical Society. 2011;76(12):1687-1701
Damnjanovic Bojana, Petrovic Biljana, Dimitric-Markovic Jasmina, Petković Marijana, "Comparison of MALDI-TOF mass spectra of [PdCl(dien)]Cl and [Ru(en)(2)Cl-2]Cl acquired with different matrices" Journal of the Serbian Chemical Society, 76, no. 12 (2011):1687-1701,
https://doi.org/10.2298/JSC110201145D .
5
5
5

Flavonoids as matrices for MALDI-TOF mass spectrometric analysis of transition metal complexes

Petković, Marijana; Petrovic, Biljana; Savić, Jasmina; Bugarcic, Zivadin D.; Dimitric-Markovic, Jasmina; Momić, Tatjana; Vasić, Vesna M.

(2010)

TY  - JOUR
AU  - Petković, Marijana
AU  - Petrovic, Biljana
AU  - Savić, Jasmina
AU  - Bugarcic, Zivadin D.
AU  - Dimitric-Markovic, Jasmina
AU  - Momić, Tatjana
AU  - Vasić, Vesna M.
PY  - 2010
UR  - http://vinar.vin.bg.ac.rs/handle/123456789/3909
AB  - Matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a suitable method for the analysis of inorganic and organic compounds and biomolecules This makes. MALDI-TOF MS convenient for monitoring the interaction of metallo-drugs with biomolecules. Results presented in this manuscript demonstrate that flavonoids such as apigenin, kaempferol and luteolin are suitable for MALDI-TOF MS analysis of Pt(II), Pd(II), Pt(IV) and Ru(III) complexes, giving different signal-to-noise ratios of the analyte peak. The MALDI-TOF mass spectra of inorganic complexes acquired with these flavonoid matrices are easy to interpret and have some advantages over the application of other commonly used matrices: a low number of matrix peaks are detectable and the coordinative metal-ligand bond is, in most cases, preserved. On the other hand, flavonoids do not act as typical matrices, as their excess is not required for the acquisition of MALDI-TOF mass spectra of inorganic complexes. (C) 2009 Elsevier B.V. All rights reserved.
T2  - International Journal of Mass Spectrometry
T1  - Flavonoids as matrices for MALDI-TOF mass spectrometric analysis of transition metal complexes
VL  - 290
IS  - 1
SP  - 39
EP  - 46
DO  - 10.1016/j.ijms.2009.12.001
ER  - 
@article{
author = "Petković, Marijana and Petrovic, Biljana and Savić, Jasmina and Bugarcic, Zivadin D. and Dimitric-Markovic, Jasmina and Momić, Tatjana and Vasić, Vesna M.",
year = "2010",
url = "http://vinar.vin.bg.ac.rs/handle/123456789/3909",
abstract = "Matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a suitable method for the analysis of inorganic and organic compounds and biomolecules This makes. MALDI-TOF MS convenient for monitoring the interaction of metallo-drugs with biomolecules. Results presented in this manuscript demonstrate that flavonoids such as apigenin, kaempferol and luteolin are suitable for MALDI-TOF MS analysis of Pt(II), Pd(II), Pt(IV) and Ru(III) complexes, giving different signal-to-noise ratios of the analyte peak. The MALDI-TOF mass spectra of inorganic complexes acquired with these flavonoid matrices are easy to interpret and have some advantages over the application of other commonly used matrices: a low number of matrix peaks are detectable and the coordinative metal-ligand bond is, in most cases, preserved. On the other hand, flavonoids do not act as typical matrices, as their excess is not required for the acquisition of MALDI-TOF mass spectra of inorganic complexes. (C) 2009 Elsevier B.V. All rights reserved.",
journal = "International Journal of Mass Spectrometry",
title = "Flavonoids as matrices for MALDI-TOF mass spectrometric analysis of transition metal complexes",
volume = "290",
number = "1",
pages = "39-46",
doi = "10.1016/j.ijms.2009.12.001"
}
Petković, M., Petrovic, B., Savić, J., Bugarcic, Z. D., Dimitric-Markovic, J., Momić, T.,& Vasić, V. M. (2010). Flavonoids as matrices for MALDI-TOF mass spectrometric analysis of transition metal complexes.
International Journal of Mass Spectrometry, 290(1), 39-46.
https://doi.org/10.1016/j.ijms.2009.12.001
Petković M, Petrovic B, Savić J, Bugarcic ZD, Dimitric-Markovic J, Momić T, Vasić VM. Flavonoids as matrices for MALDI-TOF mass spectrometric analysis of transition metal complexes. International Journal of Mass Spectrometry. 2010;290(1):39-46
Petković Marijana, Petrovic Biljana, Savić Jasmina, Bugarcic Zivadin D., Dimitric-Markovic Jasmina, Momić Tatjana, Vasić Vesna M., "Flavonoids as matrices for MALDI-TOF mass spectrometric analysis of transition metal complexes" International Journal of Mass Spectrometry, 290, no. 1 (2010):39-46,
https://doi.org/10.1016/j.ijms.2009.12.001 .
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