TY  - JOUR
AU  - Vignjevic, Sanja
AU  - Budec, Mirela
AU  - Marković, Dragana
AU  - Dikic, Dragoslava
AU  - Mitrovic, Olivera
AU  - Mojsilovic, Slavko
AU  - Vranješ-Đurić, Sanja
AU  - Koko, Vesna
AU  - Cokic, Bojana Beleslin
AU  - Cokic, Vladan
AU  - Jovcic, Gordana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5818
AB  - Psychological stress affects different physiological processes including haematopoiesis. However, erythropoietic effects of chronic psychological stress remain largely unknown. The adult spleen contains a distinct microenvironment favourable for rapid expansion of erythroid progenitors in response to stressful stimuli, and emerging evidence suggests that inappropriate activation of stress erythropoiesis may predispose to leukaemic transformation. We used a mouse model to study the influence of chronic psychological stress on erythropoiesis in the spleen and to investigate potential mediators of observed effects. Adult mice were subjected to 2hrs daily restraint stress for 7 or 14 consecutive days. Our results showed that chronic exposure to restraint stress decreased the concentration of haemoglobin in the blood, elevated circulating levels of erythropoietin and corticosterone, and resulted in markedly increased number of erythroid progenitors and precursors in the spleen. Western blot analysis revealed significantly decreased expression of both erythropoietin receptor and glucocorticoid receptor in the spleen of restrained mice. Furthermore, chronic stress enhanced the expression of stem cell factor receptor in the red pulp. Moreover, chronically stressed animals exhibited significantly increased expression of bone morphogenetic protein 4 (BMP4) in the red pulp as well as substantially enhanced mRNA expression levels of its receptors in the spleen. These findings demonstrate for the first time that chronic psychological stress activates BMP4-dependent extramedullary erythropoiesis and leads to the prolonged activation of stress erythropoiesis pathways. Prolonged activation of these pathways along with an excessive production of immature erythroid cells may predispose chronically stressed subjects to a higher risk of leukaemic transformation.
T2  - Journal of Cellular and Molecular Medicine
T1  - Chronic psychological stress activates BMP4-dependent extramedullary erythropoiesis
VL  - 18
IS  - 1
SP  - 91
EP  - 103
DO  - 10.1111/jcmm.12167
ER  - 

@article{
author = "Vignjevic, Sanja and Budec, Mirela and Marković, Dragana and Dikic, Dragoslava and Mitrovic, Olivera and Mojsilovic, Slavko and Vranješ-Đurić, Sanja and Koko, Vesna and Cokic, Bojana Beleslin and Cokic, Vladan and Jovcic, Gordana",
year = "2014",
abstract = "Psychological stress affects different physiological processes including haematopoiesis. However, erythropoietic effects of chronic psychological stress remain largely unknown. The adult spleen contains a distinct microenvironment favourable for rapid expansion of erythroid progenitors in response to stressful stimuli, and emerging evidence suggests that inappropriate activation of stress erythropoiesis may predispose to leukaemic transformation. We used a mouse model to study the influence of chronic psychological stress on erythropoiesis in the spleen and to investigate potential mediators of observed effects. Adult mice were subjected to 2hrs daily restraint stress for 7 or 14 consecutive days. Our results showed that chronic exposure to restraint stress decreased the concentration of haemoglobin in the blood, elevated circulating levels of erythropoietin and corticosterone, and resulted in markedly increased number of erythroid progenitors and precursors in the spleen. Western blot analysis revealed significantly decreased expression of both erythropoietin receptor and glucocorticoid receptor in the spleen of restrained mice. Furthermore, chronic stress enhanced the expression of stem cell factor receptor in the red pulp. Moreover, chronically stressed animals exhibited significantly increased expression of bone morphogenetic protein 4 (BMP4) in the red pulp as well as substantially enhanced mRNA expression levels of its receptors in the spleen. These findings demonstrate for the first time that chronic psychological stress activates BMP4-dependent extramedullary erythropoiesis and leads to the prolonged activation of stress erythropoiesis pathways. Prolonged activation of these pathways along with an excessive production of immature erythroid cells may predispose chronically stressed subjects to a higher risk of leukaemic transformation.",
journal = "Journal of Cellular and Molecular Medicine",
title = "Chronic psychological stress activates BMP4-dependent extramedullary erythropoiesis",
volume = "18",
number = "1",
pages = "91-103",
doi = "10.1111/jcmm.12167"
}

Vignjevic, S., Budec, M., Marković, D., Dikic, D., Mitrovic, O., Mojsilovic, S., Vranješ-Đurić, S., Koko, V., Cokic, B. B., Cokic, V.,& Jovcic, G.. (2014). Chronic psychological stress activates BMP4-dependent extramedullary erythropoiesis. in Journal of Cellular and Molecular Medicine, 18(1), 91-103.
https://doi.org/10.1111/jcmm.12167

Vignjevic S, Budec M, Marković D, Dikic D, Mitrovic O, Mojsilovic S, Vranješ-Đurić S, Koko V, Cokic BB, Cokic V, Jovcic G. Chronic psychological stress activates BMP4-dependent extramedullary erythropoiesis. in Journal of Cellular and Molecular Medicine. 2014;18(1):91-103.
doi:10.1111/jcmm.12167 .

Vignjevic, Sanja, Budec, Mirela, Marković, Dragana, Dikic, Dragoslava, Mitrovic, Olivera, Mojsilovic, Slavko, Vranješ-Đurić, Sanja, Koko, Vesna, Cokic, Bojana Beleslin, Cokic, Vladan, Jovcic, Gordana, "Chronic psychological stress activates BMP4-dependent extramedullary erythropoiesis" in Journal of Cellular and Molecular Medicine, 18, no. 1 (2014):91-103,
https://doi.org/10.1111/jcmm.12167 . .

TY  - JOUR
AU  - Dikic, Dragoslava
AU  - Budec, Mirela
AU  - Vranješ-Đurić, Sanja
AU  - Koko, Vesna
AU  - Vignjevic, Sanja
AU  - Mitrovic, Olivera
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4457
AB  - Aims: The present study was designed to investigate a possible role of endogenous nitric oxide (NO) in the adrenal response to an acute alcohol administration in female rats. To this end, N(omega)-nitro-l-arginine-methyl ester (l-NAME), a competitive inhibitor of all isoforms of NO synthase, was used. Methods: Adult female Wistar rats showing diestrus Day 1 were treated with: (a) ethanol (2 or 4 g/kg, intraperitoneally); (b) l-NAME (30 or 50 mg/kg, subcutaneously) followed by either ethanol or saline 3 h later. Untreated and saline-injected rats were used as controls. The animals were killed 30 min after last injection. Adrenal cortex was analyzed morphometrically, and plasma levels of adrenocorticotropic hormone (ACTH) and serum concentrations of corticosterone were determined. Results: Acute ethanol treatment enhanced the levels of ACTH and corticosterone in a dose-dependent manner. Stereological analysis revealed that acute alcohol administration induced a significant increase in absolute volume of the cortex and the zona fasciculata (ZF). In addition, ethanol at a dose of 4 g/kg increased volume density and length of the capillaries in the ZF. However, other stereological parameters were unaffected by alcohol exposure. Pretreatment with both doses of l-NAME had no effect on ethanol-induced changes. Conclusion: Obtained findings indicate that acute ethanol treatment stimulates the activity of the adrenal cortex and that this effect is not mediated by endogenous NO in female rats under these experimental conditions.
T2  - Alcohol and Alcoholism
T1  - The Acute Effect of Ethanol on Adrenal Cortex in Female Rats-Possible Role of Nitric Oxide
VL  - 46
IS  - 5
SP  - 523
EP  - 528
DO  - 10.1093/alcalc/agr054
ER  - 

@article{
author = "Dikic, Dragoslava and Budec, Mirela and Vranješ-Đurić, Sanja and Koko, Vesna and Vignjevic, Sanja and Mitrovic, Olivera",
year = "2011",
abstract = "Aims: The present study was designed to investigate a possible role of endogenous nitric oxide (NO) in the adrenal response to an acute alcohol administration in female rats. To this end, N(omega)-nitro-l-arginine-methyl ester (l-NAME), a competitive inhibitor of all isoforms of NO synthase, was used. Methods: Adult female Wistar rats showing diestrus Day 1 were treated with: (a) ethanol (2 or 4 g/kg, intraperitoneally); (b) l-NAME (30 or 50 mg/kg, subcutaneously) followed by either ethanol or saline 3 h later. Untreated and saline-injected rats were used as controls. The animals were killed 30 min after last injection. Adrenal cortex was analyzed morphometrically, and plasma levels of adrenocorticotropic hormone (ACTH) and serum concentrations of corticosterone were determined. Results: Acute ethanol treatment enhanced the levels of ACTH and corticosterone in a dose-dependent manner. Stereological analysis revealed that acute alcohol administration induced a significant increase in absolute volume of the cortex and the zona fasciculata (ZF). In addition, ethanol at a dose of 4 g/kg increased volume density and length of the capillaries in the ZF. However, other stereological parameters were unaffected by alcohol exposure. Pretreatment with both doses of l-NAME had no effect on ethanol-induced changes. Conclusion: Obtained findings indicate that acute ethanol treatment stimulates the activity of the adrenal cortex and that this effect is not mediated by endogenous NO in female rats under these experimental conditions.",
journal = "Alcohol and Alcoholism",
title = "The Acute Effect of Ethanol on Adrenal Cortex in Female Rats-Possible Role of Nitric Oxide",
volume = "46",
number = "5",
pages = "523-528",
doi = "10.1093/alcalc/agr054"
}

Dikic, D., Budec, M., Vranješ-Đurić, S., Koko, V., Vignjevic, S.,& Mitrovic, O.. (2011). The Acute Effect of Ethanol on Adrenal Cortex in Female Rats-Possible Role of Nitric Oxide. in Alcohol and Alcoholism, 46(5), 523-528.
https://doi.org/10.1093/alcalc/agr054

Dikic D, Budec M, Vranješ-Đurić S, Koko V, Vignjevic S, Mitrovic O. The Acute Effect of Ethanol on Adrenal Cortex in Female Rats-Possible Role of Nitric Oxide. in Alcohol and Alcoholism. 2011;46(5):523-528.
doi:10.1093/alcalc/agr054 .

Dikic, Dragoslava, Budec, Mirela, Vranješ-Đurić, Sanja, Koko, Vesna, Vignjevic, Sanja, Mitrovic, Olivera, "The Acute Effect of Ethanol on Adrenal Cortex in Female Rats-Possible Role of Nitric Oxide" in Alcohol and Alcoholism, 46, no. 5 (2011):523-528,
https://doi.org/10.1093/alcalc/agr054 . .