TY  - JOUR
AU  - Veljković, Veljko
AU  - Veljković, Nevena V.
AU  - Paessler, Slobodan
AU  - Goeijenbier, Marco
AU  - Perović, Vladimir R.
AU  - Glišić, Sanja
AU  - Muller, Claude P.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1300
AB  - Influenza A virus (IAV) subtypes against which little or no pre-existing immunity exists in humans represent a serious threat to global public health. Monitoring of IAV in animal hosts is essential for early and rapid detection of potential pandemic IAV strains to prevent their spread. Recently, the increased pandemic potential of the avian-like swine H1N1 IAV A/swine/Guangdong/104/2013 has been suggested. The virus is infectious in humans and the general population seems to lack neutralizing antibodies against this virus. Here we present an in silico analysis that shows a strong human propensity of this swine virus further confirming its pandemic potential. We suggest mutations which would further enhance its human propensity. We also propose conserved antigenic determinants which could serve as a component of a prepandemic vaccine. The bioinformatics tool, which can be used to further monitor the evolution of swine influenza viruses towards a pandemic virus, are described here and are made publically available (http://www.vin.bg.ac.rs/180/tools/iav_ mon.php;http://www.biomedprotection.com/iav_mon.php).
T2  - PLOS One
T1  - Predicted Enhanced Human Propensity of Current Avian-Like H1N1 Swine Influenza Virus from China
VL  - 11
IS  - 11
DO  - 10.1371/journal.pone.0165451
ER  - 

@article{
author = "Veljković, Veljko and Veljković, Nevena V. and Paessler, Slobodan and Goeijenbier, Marco and Perović, Vladimir R. and Glišić, Sanja and Muller, Claude P.",
year = "2016",
abstract = "Influenza A virus (IAV) subtypes against which little or no pre-existing immunity exists in humans represent a serious threat to global public health. Monitoring of IAV in animal hosts is essential for early and rapid detection of potential pandemic IAV strains to prevent their spread. Recently, the increased pandemic potential of the avian-like swine H1N1 IAV A/swine/Guangdong/104/2013 has been suggested. The virus is infectious in humans and the general population seems to lack neutralizing antibodies against this virus. Here we present an in silico analysis that shows a strong human propensity of this swine virus further confirming its pandemic potential. We suggest mutations which would further enhance its human propensity. We also propose conserved antigenic determinants which could serve as a component of a prepandemic vaccine. The bioinformatics tool, which can be used to further monitor the evolution of swine influenza viruses towards a pandemic virus, are described here and are made publically available (http://www.vin.bg.ac.rs/180/tools/iav_ mon.php;http://www.biomedprotection.com/iav_mon.php).",
journal = "PLOS One",
title = "Predicted Enhanced Human Propensity of Current Avian-Like H1N1 Swine Influenza Virus from China",
volume = "11",
number = "11",
doi = "10.1371/journal.pone.0165451"
}

Veljković, V., Veljković, N. V., Paessler, S., Goeijenbier, M., Perović, V. R., Glišić, S.,& Muller, C. P.. (2016). Predicted Enhanced Human Propensity of Current Avian-Like H1N1 Swine Influenza Virus from China. in PLOS One, 11(11).
https://doi.org/10.1371/journal.pone.0165451

Veljković V, Veljković NV, Paessler S, Goeijenbier M, Perović VR, Glišić S, Muller CP. Predicted Enhanced Human Propensity of Current Avian-Like H1N1 Swine Influenza Virus from China. in PLOS One. 2016;11(11).
doi:10.1371/journal.pone.0165451 .

Veljković, Veljko, Veljković, Nevena V., Paessler, Slobodan, Goeijenbier, Marco, Perović, Vladimir R., Glišić, Sanja, Muller, Claude P., "Predicted Enhanced Human Propensity of Current Avian-Like H1N1 Swine Influenza Virus from China" in PLOS One, 11, no. 11 (2016),
https://doi.org/10.1371/journal.pone.0165451 . .

TY  - JOUR
AU  - Veljković, Veljko
AU  - Glišić, Sanja
AU  - Muller, Claude P.
AU  - Scotch, Matthew
AU  - Branch, Donald R.
AU  - Perović, Vladimir R.
AU  - Senćanski, Milan V.
AU  - Veljković, Nevena V.
AU  - Colombatti, Alfonso
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/423
AB  - The worst Ebola virus (EV) outbreak in history has hit Liberia, Sierra Leone and Guinea hardest and the trend lines in this crisis are grave, and now represents a global public health threat concern. Limited therapeutic and/or prophylactic options are available for people suffering from Ebola virus disease (EVD) and further complicate the situation. Previous studies suggested that the EV glycoprotein (GP) is the main determinant causing structural damage of endothelial cells that triggers the hemorrhagic diathesis, but molecular mechanisms underlying this phenomenon remains elusive. Using the informational spectrum method (ISM), a virtual spectroscopy method for analysis of the protein-protein interactions, the interaction of GP with endothelial extracellular matrix (ECM) was investigated. Presented results of this in silico study suggest that Elastin Microfibril Interface Located Proteins (EMILINs) are involved in interaction between GP and ECM. This finding could contribute to a better understanding of EV/endothelium interaction and its role in pathogenesis, prevention and therapy of EVD.
T2  - Frontiers in Microbiology
T1  - In silico analysis suggests interaction between Ebola virus and the extracellular matrix
VL  - 6
DO  - 10.3389/fmicb.2015.00135
ER  - 

@article{
author = "Veljković, Veljko and Glišić, Sanja and Muller, Claude P. and Scotch, Matthew and Branch, Donald R. and Perović, Vladimir R. and Senćanski, Milan V. and Veljković, Nevena V. and Colombatti, Alfonso",
year = "2015",
abstract = "The worst Ebola virus (EV) outbreak in history has hit Liberia, Sierra Leone and Guinea hardest and the trend lines in this crisis are grave, and now represents a global public health threat concern. Limited therapeutic and/or prophylactic options are available for people suffering from Ebola virus disease (EVD) and further complicate the situation. Previous studies suggested that the EV glycoprotein (GP) is the main determinant causing structural damage of endothelial cells that triggers the hemorrhagic diathesis, but molecular mechanisms underlying this phenomenon remains elusive. Using the informational spectrum method (ISM), a virtual spectroscopy method for analysis of the protein-protein interactions, the interaction of GP with endothelial extracellular matrix (ECM) was investigated. Presented results of this in silico study suggest that Elastin Microfibril Interface Located Proteins (EMILINs) are involved in interaction between GP and ECM. This finding could contribute to a better understanding of EV/endothelium interaction and its role in pathogenesis, prevention and therapy of EVD.",
journal = "Frontiers in Microbiology",
title = "In silico analysis suggests interaction between Ebola virus and the extracellular matrix",
volume = "6",
doi = "10.3389/fmicb.2015.00135"
}

Veljković, V., Glišić, S., Muller, C. P., Scotch, M., Branch, D. R., Perović, V. R., Senćanski, M. V., Veljković, N. V.,& Colombatti, A.. (2015). In silico analysis suggests interaction between Ebola virus and the extracellular matrix. in Frontiers in Microbiology, 6.
https://doi.org/10.3389/fmicb.2015.00135

Veljković V, Glišić S, Muller CP, Scotch M, Branch DR, Perović VR, Senćanski MV, Veljković NV, Colombatti A. In silico analysis suggests interaction between Ebola virus and the extracellular matrix. in Frontiers in Microbiology. 2015;6.
doi:10.3389/fmicb.2015.00135 .

Veljković, Veljko, Glišić, Sanja, Muller, Claude P., Scotch, Matthew, Branch, Donald R., Perović, Vladimir R., Senćanski, Milan V., Veljković, Nevena V., Colombatti, Alfonso, "In silico analysis suggests interaction between Ebola virus and the extracellular matrix" in Frontiers in Microbiology, 6 (2015),
https://doi.org/10.3389/fmicb.2015.00135 . .

TY  - JOUR
AU  - Perović, Vladimir R.
AU  - Muller, Claude P.
AU  - Niman, Henry L.
AU  - Veljković, Nevena V.
AU  - Dietrich, Ursula
AU  - Tosic, Dusan D.
AU  - Glišić, Sanja
AU  - Veljković, Veljko
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5491
AB  - Years of endemic infections with highly pathogenic avian influenza (HPAI) A subtype H5N1 virus in poultry and high numbers of infections in humans provide ample opportunity in Egypt for H5N1-HPAIV to develop pandemic potential. In an effort to better understand the viral determinants that facilitate human infections of the Egyptian H5N1-HPAIVvirus, we developed a new phylogenetic algorithm based on a new distance measure derived from the informational spectrum method (ISM). This new approach, which describes functional aspects of the evolution of the hemagglutinin subunit 1 (HA1), revealed a growing group G2 of H5N1-HPAIV in Egypt after 2009 that acquired new informational spectrum (IS) properties suggestive of an increased human tropism and pandemic potential. While in 2006 all viruses in Egypt belonged to the G1 group, by 2011 these viruses were virtually replaced by G2 viruses. All of the G2 viruses displayed four characteristic mutations (D43N, S120(D,N), (S,L)129 Delta and I151T), three of which were previously reported to increase binding to the human receptor. Already in 2006-2008 G2 viruses were significantly (p LT 0.02) more often found in humans than expected from their overall prevalence and this further increased in 2009-2011 (p LT 0.007). Our approach also identified viruses that acquired additional mutations that we predict to further enhance their human tropism. The extensive evolution of Egyptian H5N1-HPAIV towards a preferential human tropism underlines an urgent need to closely monitor these viruses with respect to molecular determinants of virulence.
T2  - PLOS One
T1  - Novel Phylogenetic Algorithm to Monitor Human Tropism in Egyptian H5N1-HPAIV Reveals Evolution toward Efficient Human-to-Human Transmission
VL  - 8
IS  - 4
DO  - 10.1371/journal.pone.0061572
ER  - 

@article{
author = "Perović, Vladimir R. and Muller, Claude P. and Niman, Henry L. and Veljković, Nevena V. and Dietrich, Ursula and Tosic, Dusan D. and Glišić, Sanja and Veljković, Veljko",
year = "2013",
abstract = "Years of endemic infections with highly pathogenic avian influenza (HPAI) A subtype H5N1 virus in poultry and high numbers of infections in humans provide ample opportunity in Egypt for H5N1-HPAIV to develop pandemic potential. In an effort to better understand the viral determinants that facilitate human infections of the Egyptian H5N1-HPAIVvirus, we developed a new phylogenetic algorithm based on a new distance measure derived from the informational spectrum method (ISM). This new approach, which describes functional aspects of the evolution of the hemagglutinin subunit 1 (HA1), revealed a growing group G2 of H5N1-HPAIV in Egypt after 2009 that acquired new informational spectrum (IS) properties suggestive of an increased human tropism and pandemic potential. While in 2006 all viruses in Egypt belonged to the G1 group, by 2011 these viruses were virtually replaced by G2 viruses. All of the G2 viruses displayed four characteristic mutations (D43N, S120(D,N), (S,L)129 Delta and I151T), three of which were previously reported to increase binding to the human receptor. Already in 2006-2008 G2 viruses were significantly (p LT 0.02) more often found in humans than expected from their overall prevalence and this further increased in 2009-2011 (p LT 0.007). Our approach also identified viruses that acquired additional mutations that we predict to further enhance their human tropism. The extensive evolution of Egyptian H5N1-HPAIV towards a preferential human tropism underlines an urgent need to closely monitor these viruses with respect to molecular determinants of virulence.",
journal = "PLOS One",
title = "Novel Phylogenetic Algorithm to Monitor Human Tropism in Egyptian H5N1-HPAIV Reveals Evolution toward Efficient Human-to-Human Transmission",
volume = "8",
number = "4",
doi = "10.1371/journal.pone.0061572"
}

Perović, V. R., Muller, C. P., Niman, H. L., Veljković, N. V., Dietrich, U., Tosic, D. D., Glišić, S.,& Veljković, V.. (2013). Novel Phylogenetic Algorithm to Monitor Human Tropism in Egyptian H5N1-HPAIV Reveals Evolution toward Efficient Human-to-Human Transmission. in PLOS One, 8(4).
https://doi.org/10.1371/journal.pone.0061572

Perović VR, Muller CP, Niman HL, Veljković NV, Dietrich U, Tosic DD, Glišić S, Veljković V. Novel Phylogenetic Algorithm to Monitor Human Tropism in Egyptian H5N1-HPAIV Reveals Evolution toward Efficient Human-to-Human Transmission. in PLOS One. 2013;8(4).
doi:10.1371/journal.pone.0061572 .

Perović, Vladimir R., Muller, Claude P., Niman, Henry L., Veljković, Nevena V., Dietrich, Ursula, Tosic, Dusan D., Glišić, Sanja, Veljković, Veljko, "Novel Phylogenetic Algorithm to Monitor Human Tropism in Egyptian H5N1-HPAIV Reveals Evolution toward Efficient Human-to-Human Transmission" in PLOS One, 8, no. 4 (2013),
https://doi.org/10.1371/journal.pone.0061572 . .

TY  - JOUR
AU  - Veljković, Veljko
AU  - Veljković, Nevena V.
AU  - Muller, Claude P.
AU  - Mueller, Sybille
AU  - Glišić, Sanja
AU  - Perović, Vladimir R.
AU  - Koehler, Heinz
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3695
AB  - Background: Epidemics caused by highly pathogenic avian influenza virus (HPAIV) are a continuing threat to human health and to the worlds economy. The development of approaches, which help to understand the significance of structural changes resulting from the alarming mutational propensity for human-to-human transmission of HPAIV, is of particularly interest. Here we compare informational and structural properties of the hemagglutinin (HA) of H5N1 virus and human influenza virus subtypes, which are important for the receptor/virus interaction. Results: Presented results revealed that HA proteins encode highly conserved information that differ between influenza virus subtypes H5N1, H1N1, H3N2, H7N7 and defined an HA domain which may modulate interaction with receptor. We also found that about one third of H5N1 viruses which are isolated during the 2006/07 influenza outbreak in Egypt possibly evolve towards receptor usage similar to that of seasonal H1N1. Conclusion: The presented results may help to better understand the interaction of influenza virus with its receptor(s) and to identify new therapeutic targets for drug development.
T2  - BMC Structural Biology
T1  - Characterization of conserved properties of hemagglutinin of H5N1 and human influenza viruses: possible consequences for therapy and infection control
VL  - 9
DO  - 10.1186/1472-6807-9-21
ER  - 

@article{
author = "Veljković, Veljko and Veljković, Nevena V. and Muller, Claude P. and Mueller, Sybille and Glišić, Sanja and Perović, Vladimir R. and Koehler, Heinz",
year = "2009",
abstract = "Background: Epidemics caused by highly pathogenic avian influenza virus (HPAIV) are a continuing threat to human health and to the worlds economy. The development of approaches, which help to understand the significance of structural changes resulting from the alarming mutational propensity for human-to-human transmission of HPAIV, is of particularly interest. Here we compare informational and structural properties of the hemagglutinin (HA) of H5N1 virus and human influenza virus subtypes, which are important for the receptor/virus interaction. Results: Presented results revealed that HA proteins encode highly conserved information that differ between influenza virus subtypes H5N1, H1N1, H3N2, H7N7 and defined an HA domain which may modulate interaction with receptor. We also found that about one third of H5N1 viruses which are isolated during the 2006/07 influenza outbreak in Egypt possibly evolve towards receptor usage similar to that of seasonal H1N1. Conclusion: The presented results may help to better understand the interaction of influenza virus with its receptor(s) and to identify new therapeutic targets for drug development.",
journal = "BMC Structural Biology",
title = "Characterization of conserved properties of hemagglutinin of H5N1 and human influenza viruses: possible consequences for therapy and infection control",
volume = "9",
doi = "10.1186/1472-6807-9-21"
}

Veljković, V., Veljković, N. V., Muller, C. P., Mueller, S., Glišić, S., Perović, V. R.,& Koehler, H.. (2009). Characterization of conserved properties of hemagglutinin of H5N1 and human influenza viruses: possible consequences for therapy and infection control. in BMC Structural Biology, 9.
https://doi.org/10.1186/1472-6807-9-21

Veljković V, Veljković NV, Muller CP, Mueller S, Glišić S, Perović VR, Koehler H. Characterization of conserved properties of hemagglutinin of H5N1 and human influenza viruses: possible consequences for therapy and infection control. in BMC Structural Biology. 2009;9.
doi:10.1186/1472-6807-9-21 .

Veljković, Veljko, Veljković, Nevena V., Muller, Claude P., Mueller, Sybille, Glišić, Sanja, Perović, Vladimir R., Koehler, Heinz, "Characterization of conserved properties of hemagglutinin of H5N1 and human influenza viruses: possible consequences for therapy and infection control" in BMC Structural Biology, 9 (2009),
https://doi.org/10.1186/1472-6807-9-21 . .

TY  - JOUR
AU  - Veljković, Veljko
AU  - Niman, Henry L.
AU  - Glišić, Sanja
AU  - Veljković, Nevena V.
AU  - Perović, Vladimir R.
AU  - Muller, Claude P.
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3801
AB  - Background: The novel A/H1N1 influenza virus, which recently emerged in North America is most closely related to North American H1N1/N2 swine viruses. Until the beginning of 2009, North American swine H1N1/N2 viruses have only sporadically infected humans as dead-end hosts. In 2009 the A/H1N1 virus acquired the capacity to spread efficiently by human to human transmission. The novel A/H1N1 influenza virus has struck thousands of people in more than 70 countries and killed more than 140, representing a public health emergency of international concern. Here we have studied properties of hemagglutinin of A/H1N1 which may modulate virus/receptor interaction. Results: Analyses by ISM bioinformatics platform of the HA1 protein of North American swine H1N1/N2 viruses and the new A/H1N1 showed that both groups of viruses differed in conserved characteristics that reflect a distinct propensity of these viruses to undergo a specific interaction with swine or human host proteins or receptors. Swine H1N1/N2 viruses that sporadically infected humans featured both the swine and the human interaction pattern. Substitutions F71S, T128S, E302K, M314L in HA1 of swine H1N1 viruses from North America are identified as critical for the human interaction pattern of A/H1N1 and residues D94, D196 and D274 are predicted to be hotspots for polymorphisms which could increase infectivity of A/H1N1 virus. At least one of these residues has already emerged in the A/H1N1 isolates from Spain, Italy and USA. The domain 286-326 was identified to be involved in virus/receptor interaction. Conclusion: Our results (i) contribute to better understanding of the origin of the novel A/H1N1 influenza virus, (ii) provide a tool for monitoring its molecular evolution (iii) predicts hotspots associated with enhanced infectivity in humans and (iv) identify therapeutic and diagnostic targets for prevention and treatment of A/H1N1 infection.
T2  - BMC Structural Biology
T1  - Identification of hemagglutinin structural domain and polymorphisms which may modulate swine H1N1 interactions with human receptor
VL  - 9
DO  - 10.1186/1472-6807-9-62
ER  - 

@article{
author = "Veljković, Veljko and Niman, Henry L. and Glišić, Sanja and Veljković, Nevena V. and Perović, Vladimir R. and Muller, Claude P.",
year = "2009",
abstract = "Background: The novel A/H1N1 influenza virus, which recently emerged in North America is most closely related to North American H1N1/N2 swine viruses. Until the beginning of 2009, North American swine H1N1/N2 viruses have only sporadically infected humans as dead-end hosts. In 2009 the A/H1N1 virus acquired the capacity to spread efficiently by human to human transmission. The novel A/H1N1 influenza virus has struck thousands of people in more than 70 countries and killed more than 140, representing a public health emergency of international concern. Here we have studied properties of hemagglutinin of A/H1N1 which may modulate virus/receptor interaction. Results: Analyses by ISM bioinformatics platform of the HA1 protein of North American swine H1N1/N2 viruses and the new A/H1N1 showed that both groups of viruses differed in conserved characteristics that reflect a distinct propensity of these viruses to undergo a specific interaction with swine or human host proteins or receptors. Swine H1N1/N2 viruses that sporadically infected humans featured both the swine and the human interaction pattern. Substitutions F71S, T128S, E302K, M314L in HA1 of swine H1N1 viruses from North America are identified as critical for the human interaction pattern of A/H1N1 and residues D94, D196 and D274 are predicted to be hotspots for polymorphisms which could increase infectivity of A/H1N1 virus. At least one of these residues has already emerged in the A/H1N1 isolates from Spain, Italy and USA. The domain 286-326 was identified to be involved in virus/receptor interaction. Conclusion: Our results (i) contribute to better understanding of the origin of the novel A/H1N1 influenza virus, (ii) provide a tool for monitoring its molecular evolution (iii) predicts hotspots associated with enhanced infectivity in humans and (iv) identify therapeutic and diagnostic targets for prevention and treatment of A/H1N1 infection.",
journal = "BMC Structural Biology",
title = "Identification of hemagglutinin structural domain and polymorphisms which may modulate swine H1N1 interactions with human receptor",
volume = "9",
doi = "10.1186/1472-6807-9-62"
}

Veljković, V., Niman, H. L., Glišić, S., Veljković, N. V., Perović, V. R.,& Muller, C. P.. (2009). Identification of hemagglutinin structural domain and polymorphisms which may modulate swine H1N1 interactions with human receptor. in BMC Structural Biology, 9.
https://doi.org/10.1186/1472-6807-9-62

Veljković V, Niman HL, Glišić S, Veljković NV, Perović VR, Muller CP. Identification of hemagglutinin structural domain and polymorphisms which may modulate swine H1N1 interactions with human receptor. in BMC Structural Biology. 2009;9.
doi:10.1186/1472-6807-9-62 .

Veljković, Veljko, Niman, Henry L., Glišić, Sanja, Veljković, Nevena V., Perović, Vladimir R., Muller, Claude P., "Identification of hemagglutinin structural domain and polymorphisms which may modulate swine H1N1 interactions with human receptor" in BMC Structural Biology, 9 (2009),
https://doi.org/10.1186/1472-6807-9-62 . .