TY  - JOUR
AU  - Preljević, Kenan
AU  - Pašić, Ivana
AU  - Vlaović, Milorad
AU  - Matić, Ivana Z.
AU  - Krivokapić, Slađana
AU  - Petrović, Nina
AU  - Stanojković, Tatjana
AU  - Živković, Vladimir
AU  - Perović, Svetlana
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12935
AB  - The essential oils of Thymus vulgaris (TVEO) and Thymus serpyllum (TSEO) show different biological activities. The aim of the study was to evaluate the biological activities of TVEO and TSEO from Montenegro. The main components of TVEO were p-cymene (29.52%), thymol (22.8%) and linalool (4.73%) while the main components of TSEO were p-cymene (19.04%), geraniol (11,09%), linalool (9.16%), geranyl acetate (6.49%) and borneol (5.24%). Antioxidant activity determined via DPPH for TVEO was 4.49 and FRAP 1130.27, while for TSEO it was estimated that DPPH was 4.88 μL/mL and FRAP was 701.25 μmol FRAP/L. Both essential oils were active against all tested bacteria, with the highest level of sensitivity of E. coli with MIC of 1.5625 μL/mL. Essential oils showed strong cytotoxic effects on human cancer cell lines, with IC50 values ranging from 0.20 to 0.24 μL/mL for TVEO and from 0.32 to 0.49 μL/mL for TSEO. TVEO caused apoptosis in cervical adenocarcinoma HeLa cells through activation of caspase-3 and caspase-8, while TSEO caused apoptosis through caspase-3. EOs decreased levels of oxidative stress in normal MRC-5 cells. HeLa cells treated with TVEO had reduced MMP2 expression levels, while cells treated with TSEO had lowered MMP2 and MMP9 levels. The treatment of HeLa cells with TVEO increased the levels of miR-16 and miR-34a, indicating potential tumor-suppressive properties. Our findings suggest that Thymus essential oils may be considered as good candidates for further investigation as cancer-chemopreventive and cancer-therapeutic agents.
T2  - Fitoterapia
T1  - Comparative analysis of chemical profiles, antioxidant, antibacterial, and anticancer effects of essential oils of two Thymus species from Montenegro
VL  - 174
SP  - 105871
DO  - 10.1016/j.fitote.2024.105871
ER  - 

@article{
author = "Preljević, Kenan and Pašić, Ivana and Vlaović, Milorad and Matić, Ivana Z. and Krivokapić, Slađana and Petrović, Nina and Stanojković, Tatjana and Živković, Vladimir and Perović, Svetlana",
year = "2024",
abstract = "The essential oils of Thymus vulgaris (TVEO) and Thymus serpyllum (TSEO) show different biological activities. The aim of the study was to evaluate the biological activities of TVEO and TSEO from Montenegro. The main components of TVEO were p-cymene (29.52%), thymol (22.8%) and linalool (4.73%) while the main components of TSEO were p-cymene (19.04%), geraniol (11,09%), linalool (9.16%), geranyl acetate (6.49%) and borneol (5.24%). Antioxidant activity determined via DPPH for TVEO was 4.49 and FRAP 1130.27, while for TSEO it was estimated that DPPH was 4.88 μL/mL and FRAP was 701.25 μmol FRAP/L. Both essential oils were active against all tested bacteria, with the highest level of sensitivity of E. coli with MIC of 1.5625 μL/mL. Essential oils showed strong cytotoxic effects on human cancer cell lines, with IC50 values ranging from 0.20 to 0.24 μL/mL for TVEO and from 0.32 to 0.49 μL/mL for TSEO. TVEO caused apoptosis in cervical adenocarcinoma HeLa cells through activation of caspase-3 and caspase-8, while TSEO caused apoptosis through caspase-3. EOs decreased levels of oxidative stress in normal MRC-5 cells. HeLa cells treated with TVEO had reduced MMP2 expression levels, while cells treated with TSEO had lowered MMP2 and MMP9 levels. The treatment of HeLa cells with TVEO increased the levels of miR-16 and miR-34a, indicating potential tumor-suppressive properties. Our findings suggest that Thymus essential oils may be considered as good candidates for further investigation as cancer-chemopreventive and cancer-therapeutic agents.",
journal = "Fitoterapia",
title = "Comparative analysis of chemical profiles, antioxidant, antibacterial, and anticancer effects of essential oils of two Thymus species from Montenegro",
volume = "174",
pages = "105871",
doi = "10.1016/j.fitote.2024.105871"
}

Preljević, K., Pašić, I., Vlaović, M., Matić, I. Z., Krivokapić, S., Petrović, N., Stanojković, T., Živković, V.,& Perović, S.. (2024). Comparative analysis of chemical profiles, antioxidant, antibacterial, and anticancer effects of essential oils of two Thymus species from Montenegro. in Fitoterapia, 174, 105871.
https://doi.org/10.1016/j.fitote.2024.105871

Preljević K, Pašić I, Vlaović M, Matić IZ, Krivokapić S, Petrović N, Stanojković T, Živković V, Perović S. Comparative analysis of chemical profiles, antioxidant, antibacterial, and anticancer effects of essential oils of two Thymus species from Montenegro. in Fitoterapia. 2024;174:105871.
doi:10.1016/j.fitote.2024.105871 .

Preljević, Kenan, Pašić, Ivana, Vlaović, Milorad, Matić, Ivana Z., Krivokapić, Slađana, Petrović, Nina, Stanojković, Tatjana, Živković, Vladimir, Perović, Svetlana, "Comparative analysis of chemical profiles, antioxidant, antibacterial, and anticancer effects of essential oils of two Thymus species from Montenegro" in Fitoterapia, 174 (2024):105871,
https://doi.org/10.1016/j.fitote.2024.105871 . .

TY  - CONF
AU  - Mališić, Emina
AU  - Petrović, Nina
AU  - Nikitović, Marina
AU  - Stanojković, Tatjana
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12415
AB  - Background: Radiotherapy (RT) for prostate cancer (PCa) is associated with a spectrum of side effects (toxicity) in the surrounding normal tissues. TGFB1 is a key cytokine associated with inflammation and fibrosis, but its role in acute toxicity is unclear. The presence of T allele at −509 bp of the promoter region of TGFB1 gene is related to higher concentrations of TGFB1 than C allele. We aimed to investigate association between C-509T polymorphism (rs1800469) and RT-induced acute toxicity. Patients and methods: Eighty six patients who had a localized or locally advanced PCa were treated with radical (72 Gy)(n=49) or postoperative/salvage (66 Gy)(n=37) RT without previous hormonal therapy. TGFB1 C-509T was determined by PCR-RFLP analysis on DNA from PBMC. The differences in the distribution of genotypes between patients with or without acute genitourinary (GU) or gastrointestinal (GI) toxicity were tested by χ2 and Fisher’s exact test. P values ≤0.05 were considered statistically significant. The genotype-specific associations with toxicity were estimated as odds ratios (OR) with 95% confidence intervals (CIs) for dominant, recessive, codominant and overdominant genetic model. Results: Heterozygote carriers of TGFB1 C-509T had statistically significant lower rate of acute GU and GI toxicity then homozygotes (CC plus TT) (p=0.048; p=0.047). Additionally, the OR indicated lower risk for acute toxicity development in heterozygote than homozygote patients (OR (95%CI) were: 0.12 (0.01- 1.11) for GU and 0.19 (0.03- 1.02) for GI). Conclusion: The obtained data indicate that CT genotype of TGFB1 C-509T could be potential biomarkers of lower risk for acute RT-induced toxicity.
PB  - Belgrade : Serbian Association for Cancer Research (SDIR)
C3  - SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts
T1  - Association between TGFB1 C-509T polymorphism and acute toxicity after radiotherapy for prostate cancer
SP  - 60
EP  - 60
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12415
ER  - 

@conference{
author = "Mališić, Emina and Petrović, Nina and Nikitović, Marina and Stanojković, Tatjana",
year = "2021",
abstract = "Background: Radiotherapy (RT) for prostate cancer (PCa) is associated with a spectrum of side effects (toxicity) in the surrounding normal tissues. TGFB1 is a key cytokine associated with inflammation and fibrosis, but its role in acute toxicity is unclear. The presence of T allele at −509 bp of the promoter region of TGFB1 gene is related to higher concentrations of TGFB1 than C allele. We aimed to investigate association between C-509T polymorphism (rs1800469) and RT-induced acute toxicity. Patients and methods: Eighty six patients who had a localized or locally advanced PCa were treated with radical (72 Gy)(n=49) or postoperative/salvage (66 Gy)(n=37) RT without previous hormonal therapy. TGFB1 C-509T was determined by PCR-RFLP analysis on DNA from PBMC. The differences in the distribution of genotypes between patients with or without acute genitourinary (GU) or gastrointestinal (GI) toxicity were tested by χ2 and Fisher’s exact test. P values ≤0.05 were considered statistically significant. The genotype-specific associations with toxicity were estimated as odds ratios (OR) with 95% confidence intervals (CIs) for dominant, recessive, codominant and overdominant genetic model. Results: Heterozygote carriers of TGFB1 C-509T had statistically significant lower rate of acute GU and GI toxicity then homozygotes (CC plus TT) (p=0.048; p=0.047). Additionally, the OR indicated lower risk for acute toxicity development in heterozygote than homozygote patients (OR (95%CI) were: 0.12 (0.01- 1.11) for GU and 0.19 (0.03- 1.02) for GI). Conclusion: The obtained data indicate that CT genotype of TGFB1 C-509T could be potential biomarkers of lower risk for acute RT-induced toxicity.",
publisher = "Belgrade : Serbian Association for Cancer Research (SDIR)",
journal = "SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts",
title = "Association between TGFB1 C-509T polymorphism and acute toxicity after radiotherapy for prostate cancer",
pages = "60-60",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12415"
}

Mališić, E., Petrović, N., Nikitović, M.,& Stanojković, T.. (2021). Association between TGFB1 C-509T polymorphism and acute toxicity after radiotherapy for prostate cancer. in SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts
Belgrade : Serbian Association for Cancer Research (SDIR)., 60-60.
https://hdl.handle.net/21.15107/rcub_vinar_12415

Mališić E, Petrović N, Nikitović M, Stanojković T. Association between TGFB1 C-509T polymorphism and acute toxicity after radiotherapy for prostate cancer. in SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts. 2021;:60-60.
https://hdl.handle.net/21.15107/rcub_vinar_12415 .

Mališić, Emina, Petrović, Nina, Nikitović, Marina, Stanojković, Tatjana, "Association between TGFB1 C-509T polymorphism and acute toxicity after radiotherapy for prostate cancer" in SDIR- 5 : 5th Congress of the serbian association for cancer research : Book of abstracts (2021):60-60,
https://hdl.handle.net/21.15107/rcub_vinar_12415 .