TY  - JOUR
AU  - Pajović, Jelena D.
AU  - Dojčilović, Radovan
AU  - Kaščáková, Slávka
AU  - Réfrégiers, Matthieu
AU  - Božanić, Dušan K.
AU  - Đoković, Vladimir
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11022
AB  - Gold nanoparticles were functionalized by amino acid tryptophan and vitamin riboflavin - a resonance energy transfer (RET) pair of biomolecules. The presence of the gold nanoparticles resulted in 65% increase in RET efficiency. Because of enhanced RET efficiency, the photobleaching dynamics of the fluorescent molecules at the surface of the nanoparticles is different from that of molecules in solution. The observed effect was used for detection of the functionalized nanoparticles within biological material rich with autofluorescent species. Synchrotron radiation deep-ultraviolet fluorescence microscopy is used to study the photobleaching dynamics of the fluorescence centers within human hepatocellular carcinoma Huh7.5.1 cells incubated with the nanoparticles. The fluorescent centers were classified according to their photobleaching dynamics, which enabled the discrimination of the cell areas where the accumulation of the nanoparticles takes place, even though the particles were smaller than the spatial resolution of the images.
T2  - Colloids and Surfaces B: Biointerfaces
T1  - Enhanced resonance energy transfer in gold nanoparticles bifunctionalized by tryptophan and riboflavin and its application in fluorescence bioimaging
VL  - 227
SP  - 113340
DO  - 10.1016/j.colsurfb.2023.113340
ER  - 

@article{
author = "Pajović, Jelena D. and Dojčilović, Radovan and Kaščáková, Slávka and Réfrégiers, Matthieu and Božanić, Dušan K. and Đoković, Vladimir",
year = "2023",
abstract = "Gold nanoparticles were functionalized by amino acid tryptophan and vitamin riboflavin - a resonance energy transfer (RET) pair of biomolecules. The presence of the gold nanoparticles resulted in 65% increase in RET efficiency. Because of enhanced RET efficiency, the photobleaching dynamics of the fluorescent molecules at the surface of the nanoparticles is different from that of molecules in solution. The observed effect was used for detection of the functionalized nanoparticles within biological material rich with autofluorescent species. Synchrotron radiation deep-ultraviolet fluorescence microscopy is used to study the photobleaching dynamics of the fluorescence centers within human hepatocellular carcinoma Huh7.5.1 cells incubated with the nanoparticles. The fluorescent centers were classified according to their photobleaching dynamics, which enabled the discrimination of the cell areas where the accumulation of the nanoparticles takes place, even though the particles were smaller than the spatial resolution of the images.",
journal = "Colloids and Surfaces B: Biointerfaces",
title = "Enhanced resonance energy transfer in gold nanoparticles bifunctionalized by tryptophan and riboflavin and its application in fluorescence bioimaging",
volume = "227",
pages = "113340",
doi = "10.1016/j.colsurfb.2023.113340"
}

Pajović, J. D., Dojčilović, R., Kaščáková, S., Réfrégiers, M., Božanić, D. K.,& Đoković, V.. (2023). Enhanced resonance energy transfer in gold nanoparticles bifunctionalized by tryptophan and riboflavin and its application in fluorescence bioimaging. in Colloids and Surfaces B: Biointerfaces, 227, 113340.
https://doi.org/10.1016/j.colsurfb.2023.113340

Pajović JD, Dojčilović R, Kaščáková S, Réfrégiers M, Božanić DK, Đoković V. Enhanced resonance energy transfer in gold nanoparticles bifunctionalized by tryptophan and riboflavin and its application in fluorescence bioimaging. in Colloids and Surfaces B: Biointerfaces. 2023;227:113340.
doi:10.1016/j.colsurfb.2023.113340 .

Pajović, Jelena D., Dojčilović, Radovan, Kaščáková, Slávka, Réfrégiers, Matthieu, Božanić, Dušan K., Đoković, Vladimir, "Enhanced resonance energy transfer in gold nanoparticles bifunctionalized by tryptophan and riboflavin and its application in fluorescence bioimaging" in Colloids and Surfaces B: Biointerfaces, 227 (2023):113340,
https://doi.org/10.1016/j.colsurfb.2023.113340 . .