TY  - JOUR
AU  - Glumac, Sofija
AU  - Davidović, Radoslav S.
AU  - Dožić, Branko
AU  - Hinić, Sasa
AU  - Pavlović, Ivan
AU  - Drakulić, Dunja R.
AU  - Todorović, Ana
AU  - Medojević Pavlović, Maja
AU  - Radojević-Škodrić, Sanja
AU  - Baralić, Ivana
AU  - Sopta, Jelena
AU  - Pejić, Snežana
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9887
AB  - Rhabdomyosarcoma (RMS) is a highly malignant cancer and is the most common soft tissue sarcoma in children and adolescents, but it is rare in adults (<1% of all adult malignancies). Altered expression and molecular abnormalities of cell-cycle-regulatory proteins are one of the most prominent features in RMS. Therefore, we evaluated the expression of cyclin-dependent kinase inhibitors p57 and p16, as well as p16 methylation status, along with clinicopathological characteristics and overall survival (OS) in RMS patients. This analysis was conducted on 23 pediatric and 44 adult patients. There was a male predominance in both groups and extremities were the most frequent tumor site. In adults, alveolar and pleomorphic types were almost equally represented. The majority of pediatric tumors were low grade, whereas, in adults, only one patient had a low-grade tumor. Seven pediatric (30.43%) and eight adult (18.18%) patients had a low p16 expression. The analysis of methylation status of the p16 promoter showed the presence of methylated allele only in one sample with pleomorphic histology. Six (26.1%) pediatric and 15 (34.1%) adult patients had low p57 expression, while in 17 (73.9%) pediatric and 29 (65.9%) adult patients it was assessed as high. Ninetyone percent of the pediatric patients and 32.6% of adults were alive at the end of the observational period. In adults, significant associations were found between OS and age (P = 0.020), gender (P = 0.027), tumor size (P < 0.001), lymph node status (P < 0.001), presence of metastases (P = 0.015), and p57 expression (P = 0.039). Stratification by histological type showed the correlation of low p57 expression (P = 0.030) and worse OS of patients with alveolar RMS. Univariate analysis identified age > 50 yrs. (HR 2.447), tumors > 5 cm (HR 21.31), involvement of regional lymph nodes (HR 3.96), the presence of metastases (HR 2.53), and low p57 expression (HR 2.11) as predictors of lower OS. Tumor size, regional lymph nodes involvement, and metastases were the independent predictors after multivariate analysis, while p57 did not predict OS in an independent way. In summary, although p57 was not confirmed to be an independent predictor of OS, our results indicate that its low expression may be the marker of aggressive phenotype and poor prognosis in adult RMS patients. Also, our findings suggest that epigenetic inactivation of p16 is not important in the pathogenesis of rhabdomyosarcoma.
T2  - Pathology - Research and Practice
T1  - Immunohistochemical expression of cyclin-dependent kinase inhibitors p16 and p57 in rhabdomyosarcoma
VL  - 225
SP  - 153558
DO  - 10.1016/j.prp.2021.153558
ER  - 

@article{
author = "Glumac, Sofija and Davidović, Radoslav S. and Dožić, Branko and Hinić, Sasa and Pavlović, Ivan and Drakulić, Dunja R. and Todorović, Ana and Medojević Pavlović, Maja and Radojević-Škodrić, Sanja and Baralić, Ivana and Sopta, Jelena and Pejić, Snežana",
year = "2021",
abstract = "Rhabdomyosarcoma (RMS) is a highly malignant cancer and is the most common soft tissue sarcoma in children and adolescents, but it is rare in adults (<1% of all adult malignancies). Altered expression and molecular abnormalities of cell-cycle-regulatory proteins are one of the most prominent features in RMS. Therefore, we evaluated the expression of cyclin-dependent kinase inhibitors p57 and p16, as well as p16 methylation status, along with clinicopathological characteristics and overall survival (OS) in RMS patients. This analysis was conducted on 23 pediatric and 44 adult patients. There was a male predominance in both groups and extremities were the most frequent tumor site. In adults, alveolar and pleomorphic types were almost equally represented. The majority of pediatric tumors were low grade, whereas, in adults, only one patient had a low-grade tumor. Seven pediatric (30.43%) and eight adult (18.18%) patients had a low p16 expression. The analysis of methylation status of the p16 promoter showed the presence of methylated allele only in one sample with pleomorphic histology. Six (26.1%) pediatric and 15 (34.1%) adult patients had low p57 expression, while in 17 (73.9%) pediatric and 29 (65.9%) adult patients it was assessed as high. Ninetyone percent of the pediatric patients and 32.6% of adults were alive at the end of the observational period. In adults, significant associations were found between OS and age (P = 0.020), gender (P = 0.027), tumor size (P < 0.001), lymph node status (P < 0.001), presence of metastases (P = 0.015), and p57 expression (P = 0.039). Stratification by histological type showed the correlation of low p57 expression (P = 0.030) and worse OS of patients with alveolar RMS. Univariate analysis identified age > 50 yrs. (HR 2.447), tumors > 5 cm (HR 21.31), involvement of regional lymph nodes (HR 3.96), the presence of metastases (HR 2.53), and low p57 expression (HR 2.11) as predictors of lower OS. Tumor size, regional lymph nodes involvement, and metastases were the independent predictors after multivariate analysis, while p57 did not predict OS in an independent way. In summary, although p57 was not confirmed to be an independent predictor of OS, our results indicate that its low expression may be the marker of aggressive phenotype and poor prognosis in adult RMS patients. Also, our findings suggest that epigenetic inactivation of p16 is not important in the pathogenesis of rhabdomyosarcoma.",
journal = "Pathology - Research and Practice",
title = "Immunohistochemical expression of cyclin-dependent kinase inhibitors p16 and p57 in rhabdomyosarcoma",
volume = "225",
pages = "153558",
doi = "10.1016/j.prp.2021.153558"
}

Glumac, S., Davidović, R. S., Dožić, B., Hinić, S., Pavlović, I., Drakulić, D. R., Todorović, A., Medojević Pavlović, M., Radojević-Škodrić, S., Baralić, I., Sopta, J.,& Pejić, S.. (2021). Immunohistochemical expression of cyclin-dependent kinase inhibitors p16 and p57 in rhabdomyosarcoma. in Pathology - Research and Practice, 225, 153558.
https://doi.org/10.1016/j.prp.2021.153558

Glumac S, Davidović RS, Dožić B, Hinić S, Pavlović I, Drakulić DR, Todorović A, Medojević Pavlović M, Radojević-Škodrić S, Baralić I, Sopta J, Pejić S. Immunohistochemical expression of cyclin-dependent kinase inhibitors p16 and p57 in rhabdomyosarcoma. in Pathology - Research and Practice. 2021;225:153558.
doi:10.1016/j.prp.2021.153558 .

Glumac, Sofija, Davidović, Radoslav S., Dožić, Branko, Hinić, Sasa, Pavlović, Ivan, Drakulić, Dunja R., Todorović, Ana, Medojević Pavlović, Maja, Radojević-Škodrić, Sanja, Baralić, Ivana, Sopta, Jelena, Pejić, Snežana, "Immunohistochemical expression of cyclin-dependent kinase inhibitors p16 and p57 in rhabdomyosarcoma" in Pathology - Research and Practice, 225 (2021):153558,
https://doi.org/10.1016/j.prp.2021.153558 . .

TY  - JOUR
AU  - Petrović, Violeta
AU  - Opačić-Galić, Vanja
AU  - Jokanović, Vukoman R.
AU  - Sopta, Jelena
AU  - Prokić, Bogomir Bolka
AU  - Živković, Slavoljub
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9159
AB  - The aim of the study was to evaluate inflammatory tissue reactions and the formation of calcified tissue after implantation of experimental nanostructured calcium silicate cement (CS) and hydroxyapatite with calcium silicate cement (HA-CS) into root canals of rabbits' teeth. The study was conducted on four rabbits of the genus Oryctolagus cuniculus. After instrumentation and irrigation, the root canals of the central incisors were dried and filled with CS, HA-CS and control material (MTA Angelus). The animals were sacrificed after 28 days. After histological preparation and hematoxylin-eosin staining, tissue samples were evaluated for the intensity and extension of inflammatory tissue reaction; continuity, morphology and thickness of the newly formed calcified tissue; and presence of giant cells, materials particles and microorganisms. Kruskal Wallis and Dunn's post hoc test were used for data analysis (α=0.05). There were no significant differences in the intensity of inflammatory reactions between CS, HA-CS and MTA control. HA-CS showed significantly better results than MTA and CS with respect to continuity of the newly formed calcified tissue (P=0.003 and P=0.010, respectively). Significant differences in thickness of the calcified tissue existed between CS and MTA (P=0.004) and between HA-CS and MTA (P=0.012). Application of CS and HA-CS resulted in minimal inflammatory tissue response, similar to the MTA control. CS and HA-CS were more efficient than MTA in supporting hard tissue formation. The best organized newly formed calcified tissue was seen after HA-CS application.
AB  - Cilj rada je bio da se ispitaju Kanali korena centralnih sekutića su posle instrumentacije, ispiranja i sušenja napunjeni materijalima CS, HA-CS i kontrolnim materijalom, mineral trioksid agregatom (MTA). Životinje su žrtvovane posle 28 dana. Posle histološke pripreme, uzorci tkiva su analizirani u pogledu inteziteta i raširenosti zapaljenske reakcije; kontinuiteta, morfologije i debljine novoformiranog kalcifi kovanog tkiva; prisustva džinovskih ćelija, čestica materijala i mikroorganizama. Dobijeni rezultati su statistički obrađeni (α = 0,05) Nisu uočene statistički značajne razlike u intezitetu zapaljenske reakcije između CS, HA-CS i MTA. U pogledu kontinuiteta novostvorenog kalcifi kovanog tkiva HA-CS je pokazao bolje rezultate u odnosu na MTA i CS (p=0,003 i p=0,010). Značajne razlike utvrđene su u pogledu debljine kalcifi kovanog tkiva između CS i MTA (p=0,004), kao i HA-CS i MTA (p=0,012). Aplikacija materijala CS i HA-CS je rezultirala minimalnom zapaljenskom reakcijom tkiva, slično kontrolnom materijalu (MTA). CS i HA-CS su bili efi kasniji u pogledu stimulacije formiranja kalcifi kovanog tkiva u odnosu na MTA. Najbolje organizovano novoformirano tkivo uočeno je posle aplikacije materijala HA-CS. zapaljenske reakcije u periradikularnom tkivu i formiranje kalcifi kovanog tkiva posle implantacije eksperimentalnih, nanostrukturnih cemenata na bazi kalcijum silikata (CS) i mešavine hidroksiapatita i kalcijum silikata (HA-CS) u kanale korena zuba kunića.
T2  - Acta Veterinaria, Beograd
T1  - Histological Evaluation of Periradicular Tissue Inflammatory Reactions and Calcified Tissue Formations after Implantation of Experimental Calcium Silicate and Hydroxyapatite Based Nanostructural Cements into Root Canals of Rabbits Teeth
T1  - Histološka analiza zapaljenskih reakcija u periradikularnom tkivu i formiranja kalcifikovanog tkiva posle implantacije eksperimentalnih nanostrukturnih cemenata na bazi kalcijum silikata i hidroksiapatita u kanale korena zuba kunića
VL  - 71
IS  - 1
SP  - 85
EP  - 97
DO  - 10.2478/acve-2021-0006
ER  - 

@article{
author = "Petrović, Violeta and Opačić-Galić, Vanja and Jokanović, Vukoman R. and Sopta, Jelena and Prokić, Bogomir Bolka and Živković, Slavoljub",
year = "2021",
abstract = "The aim of the study was to evaluate inflammatory tissue reactions and the formation of calcified tissue after implantation of experimental nanostructured calcium silicate cement (CS) and hydroxyapatite with calcium silicate cement (HA-CS) into root canals of rabbits' teeth. The study was conducted on four rabbits of the genus Oryctolagus cuniculus. After instrumentation and irrigation, the root canals of the central incisors were dried and filled with CS, HA-CS and control material (MTA Angelus). The animals were sacrificed after 28 days. After histological preparation and hematoxylin-eosin staining, tissue samples were evaluated for the intensity and extension of inflammatory tissue reaction; continuity, morphology and thickness of the newly formed calcified tissue; and presence of giant cells, materials particles and microorganisms. Kruskal Wallis and Dunn's post hoc test were used for data analysis (α=0.05). There were no significant differences in the intensity of inflammatory reactions between CS, HA-CS and MTA control. HA-CS showed significantly better results than MTA and CS with respect to continuity of the newly formed calcified tissue (P=0.003 and P=0.010, respectively). Significant differences in thickness of the calcified tissue existed between CS and MTA (P=0.004) and between HA-CS and MTA (P=0.012). Application of CS and HA-CS resulted in minimal inflammatory tissue response, similar to the MTA control. CS and HA-CS were more efficient than MTA in supporting hard tissue formation. The best organized newly formed calcified tissue was seen after HA-CS application., Cilj rada je bio da se ispitaju Kanali korena centralnih sekutića su posle instrumentacije, ispiranja i sušenja napunjeni materijalima CS, HA-CS i kontrolnim materijalom, mineral trioksid agregatom (MTA). Životinje su žrtvovane posle 28 dana. Posle histološke pripreme, uzorci tkiva su analizirani u pogledu inteziteta i raširenosti zapaljenske reakcije; kontinuiteta, morfologije i debljine novoformiranog kalcifi kovanog tkiva; prisustva džinovskih ćelija, čestica materijala i mikroorganizama. Dobijeni rezultati su statistički obrađeni (α = 0,05) Nisu uočene statistički značajne razlike u intezitetu zapaljenske reakcije između CS, HA-CS i MTA. U pogledu kontinuiteta novostvorenog kalcifi kovanog tkiva HA-CS je pokazao bolje rezultate u odnosu na MTA i CS (p=0,003 i p=0,010). Značajne razlike utvrđene su u pogledu debljine kalcifi kovanog tkiva između CS i MTA (p=0,004), kao i HA-CS i MTA (p=0,012). Aplikacija materijala CS i HA-CS je rezultirala minimalnom zapaljenskom reakcijom tkiva, slično kontrolnom materijalu (MTA). CS i HA-CS su bili efi kasniji u pogledu stimulacije formiranja kalcifi kovanog tkiva u odnosu na MTA. Najbolje organizovano novoformirano tkivo uočeno je posle aplikacije materijala HA-CS. zapaljenske reakcije u periradikularnom tkivu i formiranje kalcifi kovanog tkiva posle implantacije eksperimentalnih, nanostrukturnih cemenata na bazi kalcijum silikata (CS) i mešavine hidroksiapatita i kalcijum silikata (HA-CS) u kanale korena zuba kunića.",
journal = "Acta Veterinaria, Beograd",
title = "Histological Evaluation of Periradicular Tissue Inflammatory Reactions and Calcified Tissue Formations after Implantation of Experimental Calcium Silicate and Hydroxyapatite Based Nanostructural Cements into Root Canals of Rabbits Teeth, Histološka analiza zapaljenskih reakcija u periradikularnom tkivu i formiranja kalcifikovanog tkiva posle implantacije eksperimentalnih nanostrukturnih cemenata na bazi kalcijum silikata i hidroksiapatita u kanale korena zuba kunića",
volume = "71",
number = "1",
pages = "85-97",
doi = "10.2478/acve-2021-0006"
}

Petrović, V., Opačić-Galić, V., Jokanović, V. R., Sopta, J., Prokić, B. B.,& Živković, S.. (2021). Histological Evaluation of Periradicular Tissue Inflammatory Reactions and Calcified Tissue Formations after Implantation of Experimental Calcium Silicate and Hydroxyapatite Based Nanostructural Cements into Root Canals of Rabbits Teeth. in Acta Veterinaria, Beograd, 71(1), 85-97.
https://doi.org/10.2478/acve-2021-0006

Petrović V, Opačić-Galić V, Jokanović VR, Sopta J, Prokić BB, Živković S. Histological Evaluation of Periradicular Tissue Inflammatory Reactions and Calcified Tissue Formations after Implantation of Experimental Calcium Silicate and Hydroxyapatite Based Nanostructural Cements into Root Canals of Rabbits Teeth. in Acta Veterinaria, Beograd. 2021;71(1):85-97.
doi:10.2478/acve-2021-0006 .

Petrović, Violeta, Opačić-Galić, Vanja, Jokanović, Vukoman R., Sopta, Jelena, Prokić, Bogomir Bolka, Živković, Slavoljub, "Histological Evaluation of Periradicular Tissue Inflammatory Reactions and Calcified Tissue Formations after Implantation of Experimental Calcium Silicate and Hydroxyapatite Based Nanostructural Cements into Root Canals of Rabbits Teeth" in Acta Veterinaria, Beograd, 71, no. 1 (2021):85-97,
https://doi.org/10.2478/acve-2021-0006 . .

TY  - JOUR
AU  - Jokanović, Vukoman R.
AU  - Čolović, Božana M.
AU  - Marković, Dejan
AU  - Petrović, Milan
AU  - Soldatovic, Ivan
AU  - Antonijević, Đorđe
AU  - Milosavljević, Petar
AU  - Sjerobabin, Nikola
AU  - Sopta, Jelena
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1604
AB  - This study examined the potential of a new porous calcium hydroxyapatite scaffold covered with poly (lactide-co-glycolide) (PLGA) as a bone substitute, identifying its advantages over Geistlich Bio-Oss (R), considered the gold standard, in in vivo biofunctionality investigations. Structural and morphological properties of the new scaffold were analyzed by scanning electron and atomic force microscopy. The biofunctionality assays were performed on New Zealand white rabbits using new scaffold for filling full-thickness defects of critical size. The evaluated parameters were: the presence of macrophages, giant cells, monoocytes, plasma cells, granulocytes, neo-angiogenesis, fibroplasia, and the percentage of mineralization. Parallel biofunctionality assays were performed using Geistlich Bio-Oss (R). The appearance of bone defects 12 weeks after the new scaffold implantation showed the presence of a small number of typical immune response cells. Furthermore, significantly reduced number of capillary buds, low intensity of fibroplasia and high degree of mineralization in a lamellar pattern indicated that the inflammation process has been almost completely overcome and that the new bone formed was in the final phase of remodeling. All biofunctionality assays proved the new scaffolds suitability as a bone substitute for applications in maxillofacial surgery. It showed numerous biological advantages over Geistlich Bio-Oss (R) which was reflected mainly as a lower number of giant cells surrounding implanted material and higher degree of mineralization in new formed bone.
T2  - Biomedical Engineering / Biomedizinische Technik
T1  - Extraordinary biological properties of a new calcium hydroxyapatite/poly(lactide-co-glycolide)-based scaffold confirmed by in vivo investigation
VL  - 62
IS  - 3
SP  - 295
EP  - 306
DO  - 10.1515/bmt-2015-0164
ER  - 

@article{
author = "Jokanović, Vukoman R. and Čolović, Božana M. and Marković, Dejan and Petrović, Milan and Soldatovic, Ivan and Antonijević, Đorđe and Milosavljević, Petar and Sjerobabin, Nikola and Sopta, Jelena",
year = "2017",
abstract = "This study examined the potential of a new porous calcium hydroxyapatite scaffold covered with poly (lactide-co-glycolide) (PLGA) as a bone substitute, identifying its advantages over Geistlich Bio-Oss (R), considered the gold standard, in in vivo biofunctionality investigations. Structural and morphological properties of the new scaffold were analyzed by scanning electron and atomic force microscopy. The biofunctionality assays were performed on New Zealand white rabbits using new scaffold for filling full-thickness defects of critical size. The evaluated parameters were: the presence of macrophages, giant cells, monoocytes, plasma cells, granulocytes, neo-angiogenesis, fibroplasia, and the percentage of mineralization. Parallel biofunctionality assays were performed using Geistlich Bio-Oss (R). The appearance of bone defects 12 weeks after the new scaffold implantation showed the presence of a small number of typical immune response cells. Furthermore, significantly reduced number of capillary buds, low intensity of fibroplasia and high degree of mineralization in a lamellar pattern indicated that the inflammation process has been almost completely overcome and that the new bone formed was in the final phase of remodeling. All biofunctionality assays proved the new scaffolds suitability as a bone substitute for applications in maxillofacial surgery. It showed numerous biological advantages over Geistlich Bio-Oss (R) which was reflected mainly as a lower number of giant cells surrounding implanted material and higher degree of mineralization in new formed bone.",
journal = "Biomedical Engineering / Biomedizinische Technik",
title = "Extraordinary biological properties of a new calcium hydroxyapatite/poly(lactide-co-glycolide)-based scaffold confirmed by in vivo investigation",
volume = "62",
number = "3",
pages = "295-306",
doi = "10.1515/bmt-2015-0164"
}

Jokanović, V. R., Čolović, B. M., Marković, D., Petrović, M., Soldatovic, I., Antonijević, Đ., Milosavljević, P., Sjerobabin, N.,& Sopta, J.. (2017). Extraordinary biological properties of a new calcium hydroxyapatite/poly(lactide-co-glycolide)-based scaffold confirmed by in vivo investigation. in Biomedical Engineering / Biomedizinische Technik, 62(3), 295-306.
https://doi.org/10.1515/bmt-2015-0164

Jokanović VR, Čolović BM, Marković D, Petrović M, Soldatovic I, Antonijević Đ, Milosavljević P, Sjerobabin N, Sopta J. Extraordinary biological properties of a new calcium hydroxyapatite/poly(lactide-co-glycolide)-based scaffold confirmed by in vivo investigation. in Biomedical Engineering / Biomedizinische Technik. 2017;62(3):295-306.
doi:10.1515/bmt-2015-0164 .

Jokanović, Vukoman R., Čolović, Božana M., Marković, Dejan, Petrović, Milan, Soldatovic, Ivan, Antonijević, Đorđe, Milosavljević, Petar, Sjerobabin, Nikola, Sopta, Jelena, "Extraordinary biological properties of a new calcium hydroxyapatite/poly(lactide-co-glycolide)-based scaffold confirmed by in vivo investigation" in Biomedical Engineering / Biomedizinische Technik, 62, no. 3 (2017):295-306,
https://doi.org/10.1515/bmt-2015-0164 . .

TY  - JOUR
AU  - Jokanović, Vukoman R.
AU  - Čolović, Božana M.
AU  - Marković, Dejan
AU  - Petrović, Milan
AU  - Jokanović, Milan
AU  - Milosavljević, Petar
AU  - Sopta, Jelena
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1040
AB  - A synthetic bone substitute based on calcium hydroxyapatite (CHA) and poly(lactic-co-glycolic) acid (PLGA), described in this paper, was synthesized to fulfill specific requirements like biodegradability, satisfying mechanical properties, optimal porosity and nanotopology, osteoinductive and osteoconductive properties, and so forth. Structural and morphological properties of the new scaffold were analyzed by micro computed tomography and scanning electron microscopy, while its physicochemical properties were investigated by X-ray diffraction and infrared spectroscopy. In vivo biological investigations of the synthesized scaffold were conducted over the cutaneous irritation and biofunctionality assays on rabbits and the test of acute systemic toxicity on mice. The results showed that the scaffold is not irritant and that it does not exhibit any symptoms of acute toxicity. Biofunctionality assays which include evaluation of the presence of various cells of immune response, the presence of neoangiogenesis, percentage of mineralization of newly formed bone, and fibroplasia in the tissue indicated that the new scaffold is suitable for the application in maxillofacial and dental surgery as a bone substitute. Also, it showed significant advantages over commercial product Geistlich Bio-Oss(R) from the aspect of some parameters of immunological response.
T2  - Journal of Nanomaterials
T1  - In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA
DO  - 10.1155/2016/3948768
ER  - 

@article{
author = "Jokanović, Vukoman R. and Čolović, Božana M. and Marković, Dejan and Petrović, Milan and Jokanović, Milan and Milosavljević, Petar and Sopta, Jelena",
year = "2016",
abstract = "A synthetic bone substitute based on calcium hydroxyapatite (CHA) and poly(lactic-co-glycolic) acid (PLGA), described in this paper, was synthesized to fulfill specific requirements like biodegradability, satisfying mechanical properties, optimal porosity and nanotopology, osteoinductive and osteoconductive properties, and so forth. Structural and morphological properties of the new scaffold were analyzed by micro computed tomography and scanning electron microscopy, while its physicochemical properties were investigated by X-ray diffraction and infrared spectroscopy. In vivo biological investigations of the synthesized scaffold were conducted over the cutaneous irritation and biofunctionality assays on rabbits and the test of acute systemic toxicity on mice. The results showed that the scaffold is not irritant and that it does not exhibit any symptoms of acute toxicity. Biofunctionality assays which include evaluation of the presence of various cells of immune response, the presence of neoangiogenesis, percentage of mineralization of newly formed bone, and fibroplasia in the tissue indicated that the new scaffold is suitable for the application in maxillofacial and dental surgery as a bone substitute. Also, it showed significant advantages over commercial product Geistlich Bio-Oss(R) from the aspect of some parameters of immunological response.",
journal = "Journal of Nanomaterials",
title = "In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA",
doi = "10.1155/2016/3948768"
}

Jokanović, V. R., Čolović, B. M., Marković, D., Petrović, M., Jokanović, M., Milosavljević, P.,& Sopta, J.. (2016). In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA. in Journal of Nanomaterials.
https://doi.org/10.1155/2016/3948768

Jokanović VR, Čolović BM, Marković D, Petrović M, Jokanović M, Milosavljević P, Sopta J. In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA. in Journal of Nanomaterials. 2016;.
doi:10.1155/2016/3948768 .

Jokanović, Vukoman R., Čolović, Božana M., Marković, Dejan, Petrović, Milan, Jokanović, Milan, Milosavljević, Petar, Sopta, Jelena, "In Vivo Investigation of ALBO-OS Scaffold Based on Hydroxyapatite and PLGA" in Journal of Nanomaterials (2016),
https://doi.org/10.1155/2016/3948768 . .

TY  - JOUR
AU  - Sopta, Jelena
AU  - Lujić, Nenad
AU  - Kovačević, Relja
AU  - Davidović, Radoslav S.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1429
AB  - Aim of the study is to determine the possible roles of p53, cyclin D1, B-catenin and Ki-67 in the increase in risk of fractures in patients with giant cell tumor of bone. The study included a total of 164 patients with giant cell tumor of bone (GCTB), 21 (12.8%) with and 143 (87.2%) without fracture. The samples were analyzed immunohistochemically for expression of Ki-67, p53, cyclin D1 and beta-catenin. According to the immunohistochemical expression of p53 and Ki-67 in mononuclear stromal cells, as well as of cyclin D1 in multinuclear giant cells, there was no significant association with immunopositivity and risk of fractures. However, our research revealed that patients with cytoplasmic expression of beta-catenin in stromal cells had three times more frequent occurrence of pathological fractures, which was highly statistically significant (chi(2) = 7.065; p = 0.008). Moreover, a highly statistically significant correlation between the nuclear expression of beta-catenin in giant cells and the incidence of pathological fractures was also found (chi(2) = 8.824; p = 0.003). The study showed that beta-catenin expression highly correlates with the incidence of pathological fractures in patients with GCTB. Taking into account that beta-catenin is closely linked to activation of the Wnt signaling pathway in GCTB pathogenesis, one could postulate that activation of the Wnt pathway is one of the contributing factors to locally destructive behavior of this tumor, as well as to the incidence of pathological fractures.
T2  - Polish Journal of Pathology
T1  - Significance of Beta-Catenin Expression for the Incidence of Pathological Fractures in Giant Cell Tumors of Bone
VL  - 67
IS  - 4
SP  - 345
EP  - 350
DO  - 10.5114/PJP.2016.65866
ER  - 

@article{
author = "Sopta, Jelena and Lujić, Nenad and Kovačević, Relja and Davidović, Radoslav S.",
year = "2016",
abstract = "Aim of the study is to determine the possible roles of p53, cyclin D1, B-catenin and Ki-67 in the increase in risk of fractures in patients with giant cell tumor of bone. The study included a total of 164 patients with giant cell tumor of bone (GCTB), 21 (12.8%) with and 143 (87.2%) without fracture. The samples were analyzed immunohistochemically for expression of Ki-67, p53, cyclin D1 and beta-catenin. According to the immunohistochemical expression of p53 and Ki-67 in mononuclear stromal cells, as well as of cyclin D1 in multinuclear giant cells, there was no significant association with immunopositivity and risk of fractures. However, our research revealed that patients with cytoplasmic expression of beta-catenin in stromal cells had three times more frequent occurrence of pathological fractures, which was highly statistically significant (chi(2) = 7.065; p = 0.008). Moreover, a highly statistically significant correlation between the nuclear expression of beta-catenin in giant cells and the incidence of pathological fractures was also found (chi(2) = 8.824; p = 0.003). The study showed that beta-catenin expression highly correlates with the incidence of pathological fractures in patients with GCTB. Taking into account that beta-catenin is closely linked to activation of the Wnt signaling pathway in GCTB pathogenesis, one could postulate that activation of the Wnt pathway is one of the contributing factors to locally destructive behavior of this tumor, as well as to the incidence of pathological fractures.",
journal = "Polish Journal of Pathology",
title = "Significance of Beta-Catenin Expression for the Incidence of Pathological Fractures in Giant Cell Tumors of Bone",
volume = "67",
number = "4",
pages = "345-350",
doi = "10.5114/PJP.2016.65866"
}

Sopta, J., Lujić, N., Kovačević, R.,& Davidović, R. S.. (2016). Significance of Beta-Catenin Expression for the Incidence of Pathological Fractures in Giant Cell Tumors of Bone. in Polish Journal of Pathology, 67(4), 345-350.
https://doi.org/10.5114/PJP.2016.65866

Sopta J, Lujić N, Kovačević R, Davidović RS. Significance of Beta-Catenin Expression for the Incidence of Pathological Fractures in Giant Cell Tumors of Bone. in Polish Journal of Pathology. 2016;67(4):345-350.
doi:10.5114/PJP.2016.65866 .

Sopta, Jelena, Lujić, Nenad, Kovačević, Relja, Davidović, Radoslav S., "Significance of Beta-Catenin Expression for the Incidence of Pathological Fractures in Giant Cell Tumors of Bone" in Polish Journal of Pathology, 67, no. 4 (2016):345-350,
https://doi.org/10.5114/PJP.2016.65866 . .

TY  - JOUR
AU  - Lujić, Nenad
AU  - Sopta, Jelena
AU  - Kovačević, Relja
AU  - Stevanović, Vladan
AU  - Davidović, Radoslav S.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1298
AB  - To determine various clinical, radiographic, and pathological parameters which may indicate an increased risk of Giant cell tumour of bone (GCTB) recurrence after surgical therapy. The study included a total of 164 GCTB samples; 118 (72 %) primary tumours, and 46 (28 %) recurrences; which were analyzed on immunohistochemistry for expression of Ki67, p53, cyclin D1, and beta-catenin. Among 13 analyzed clinical, radiological, and histological variables, which presented possible predictive factors for the incidence of GCTB relapse, univariate logistic regression (ULR) extract three highly statistically significant parameters: 1) lesion localization, 2) nuclear p53 expression in mononuclear cells, and 3) nuclear cyclin D1 expression in giant multinuclear cells. The multivariate logistic regression (MLR), revealing that p53 expression in mononuclear cells was the most significant predictive factor (HR = 6,181 p LT 0,001), the positivity of which indicated six times higher probability for recurrence in GCTB. The expression of cyclin D1 in giant cells, containing less than 15 nuclei, was also statistically significant (HR = 8,398, p = 0,038) for predicting the recurrence, and demonstrated eight times more frequent recurrence in positive tumours. This study confirmed independent predicting factors for GCTB reccurence: p53 expression in mononuclear tumour cells and cyclin D1 expression in giant multinuclear cells. Results are new addition to generally known parameters, such as: localization of lesion, number of surgical interventions, clear destruction of cortex with the presence of extracompartmental lesion, and histological criteria for malignancy and can help in further research and treatment of GCTB.
T2  - International Orthopaedics
T1  - Recurrence of giant cell tumour of bone: role of p53, cyclin D1, beta-catenin and Ki67
VL  - 40
IS  - 11
SP  - 2393
EP  - 2399
DO  - 10.1007/s00264-016-3292-2
ER  - 

@article{
author = "Lujić, Nenad and Sopta, Jelena and Kovačević, Relja and Stevanović, Vladan and Davidović, Radoslav S.",
year = "2016",
abstract = "To determine various clinical, radiographic, and pathological parameters which may indicate an increased risk of Giant cell tumour of bone (GCTB) recurrence after surgical therapy. The study included a total of 164 GCTB samples; 118 (72 %) primary tumours, and 46 (28 %) recurrences; which were analyzed on immunohistochemistry for expression of Ki67, p53, cyclin D1, and beta-catenin. Among 13 analyzed clinical, radiological, and histological variables, which presented possible predictive factors for the incidence of GCTB relapse, univariate logistic regression (ULR) extract three highly statistically significant parameters: 1) lesion localization, 2) nuclear p53 expression in mononuclear cells, and 3) nuclear cyclin D1 expression in giant multinuclear cells. The multivariate logistic regression (MLR), revealing that p53 expression in mononuclear cells was the most significant predictive factor (HR = 6,181 p LT 0,001), the positivity of which indicated six times higher probability for recurrence in GCTB. The expression of cyclin D1 in giant cells, containing less than 15 nuclei, was also statistically significant (HR = 8,398, p = 0,038) for predicting the recurrence, and demonstrated eight times more frequent recurrence in positive tumours. This study confirmed independent predicting factors for GCTB reccurence: p53 expression in mononuclear tumour cells and cyclin D1 expression in giant multinuclear cells. Results are new addition to generally known parameters, such as: localization of lesion, number of surgical interventions, clear destruction of cortex with the presence of extracompartmental lesion, and histological criteria for malignancy and can help in further research and treatment of GCTB.",
journal = "International Orthopaedics",
title = "Recurrence of giant cell tumour of bone: role of p53, cyclin D1, beta-catenin and Ki67",
volume = "40",
number = "11",
pages = "2393-2399",
doi = "10.1007/s00264-016-3292-2"
}

Lujić, N., Sopta, J., Kovačević, R., Stevanović, V.,& Davidović, R. S.. (2016). Recurrence of giant cell tumour of bone: role of p53, cyclin D1, beta-catenin and Ki67. in International Orthopaedics, 40(11), 2393-2399.
https://doi.org/10.1007/s00264-016-3292-2

Lujić N, Sopta J, Kovačević R, Stevanović V, Davidović RS. Recurrence of giant cell tumour of bone: role of p53, cyclin D1, beta-catenin and Ki67. in International Orthopaedics. 2016;40(11):2393-2399.
doi:10.1007/s00264-016-3292-2 .

Lujić, Nenad, Sopta, Jelena, Kovačević, Relja, Stevanović, Vladan, Davidović, Radoslav S., "Recurrence of giant cell tumour of bone: role of p53, cyclin D1, beta-catenin and Ki67" in International Orthopaedics, 40, no. 11 (2016):2393-2399,
https://doi.org/10.1007/s00264-016-3292-2 . .

TY  - JOUR
AU  - Davidović, Radoslav S.
AU  - Sopta, Jelena
AU  - Mandušić, Vesna
AU  - Krajnović, Milena M.
AU  - Stanojevic, Maja
AU  - Tulic, Goran
AU  - Dimitrijević, Bogomir B.
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5646
AB  - Liposarcoma represents the most frequent group of soft tissue sarcomas. The group can be divided into three different classes: (1) differentiated/undifferentiated (WDLPS/DDLPS), (2) myxoid/round cell (MLPS/RCLPS) and (3) pleomorphic liposarcoma (PLS). It has become apparent that p53-p14 and Rb-p16 pathways play important roles in the pathogenesis of various sarcoma types. Molecular studies of the genes involved in these two pathways showed wide variations between the liposarcoma subtypes or even within the same subtype. We sought to examine mutational status of p53 and methylation status of p16(INK4a)/p14(ARF) genes in primary and recurrent liposarcoma tumors. There were twelve myxoid (12/18, 66.7 %) and six pleomorphic liposarcoma (6/18, 33.3 %) samples. Immunohistochemical analysis revealed that p53 protein was overexpressed in 3/12 MLPS (25 %) and 6/6 PLS (100 %). Mutational analysis showed that 2/11 MLPS (18.2 %) and 2/6 PLS (33.3 %) contained mutated p53 gene. On the other hand, 3/18 samples (16.7 %) had methylated p16 promoter. However, the frequencies of the p14(ARF) gene methylation were 83.3 % (10/12) and 50 % (3/6) in myxoid and pleomorphic group, respectively. Overall, 15 out of 18 (83.3 %) samples had either p53 gene mutation or methylated p14(ARF) promoter. The results from the current study suggest significant impact of the p14(ARF) gene methylation on the pathogenesis and progression of myxoid and to a lesser extent pleomorphic liposarcoma. Despite the limited number of samples, our study points to necessity of further investigation of p53-p14 and Rb-p16 pathways in liposarcoma.
T2  - Medical Oncology
T1  - p14(ARF) methylation is a common event in the pathogenesis and progression of myxoid and pleomorphic liposarcoma
VL  - 30
IS  - 3
DO  - 10.1007/s12032-013-0682-9
ER  - 

@article{
author = "Davidović, Radoslav S. and Sopta, Jelena and Mandušić, Vesna and Krajnović, Milena M. and Stanojevic, Maja and Tulic, Goran and Dimitrijević, Bogomir B.",
year = "2013",
abstract = "Liposarcoma represents the most frequent group of soft tissue sarcomas. The group can be divided into three different classes: (1) differentiated/undifferentiated (WDLPS/DDLPS), (2) myxoid/round cell (MLPS/RCLPS) and (3) pleomorphic liposarcoma (PLS). It has become apparent that p53-p14 and Rb-p16 pathways play important roles in the pathogenesis of various sarcoma types. Molecular studies of the genes involved in these two pathways showed wide variations between the liposarcoma subtypes or even within the same subtype. We sought to examine mutational status of p53 and methylation status of p16(INK4a)/p14(ARF) genes in primary and recurrent liposarcoma tumors. There were twelve myxoid (12/18, 66.7 %) and six pleomorphic liposarcoma (6/18, 33.3 %) samples. Immunohistochemical analysis revealed that p53 protein was overexpressed in 3/12 MLPS (25 %) and 6/6 PLS (100 %). Mutational analysis showed that 2/11 MLPS (18.2 %) and 2/6 PLS (33.3 %) contained mutated p53 gene. On the other hand, 3/18 samples (16.7 %) had methylated p16 promoter. However, the frequencies of the p14(ARF) gene methylation were 83.3 % (10/12) and 50 % (3/6) in myxoid and pleomorphic group, respectively. Overall, 15 out of 18 (83.3 %) samples had either p53 gene mutation or methylated p14(ARF) promoter. The results from the current study suggest significant impact of the p14(ARF) gene methylation on the pathogenesis and progression of myxoid and to a lesser extent pleomorphic liposarcoma. Despite the limited number of samples, our study points to necessity of further investigation of p53-p14 and Rb-p16 pathways in liposarcoma.",
journal = "Medical Oncology",
title = "p14(ARF) methylation is a common event in the pathogenesis and progression of myxoid and pleomorphic liposarcoma",
volume = "30",
number = "3",
doi = "10.1007/s12032-013-0682-9"
}

Davidović, R. S., Sopta, J., Mandušić, V., Krajnović, M. M., Stanojevic, M., Tulic, G.,& Dimitrijević, B. B.. (2013). p14(ARF) methylation is a common event in the pathogenesis and progression of myxoid and pleomorphic liposarcoma. in Medical Oncology, 30(3).
https://doi.org/10.1007/s12032-013-0682-9

Davidović RS, Sopta J, Mandušić V, Krajnović MM, Stanojevic M, Tulic G, Dimitrijević BB. p14(ARF) methylation is a common event in the pathogenesis and progression of myxoid and pleomorphic liposarcoma. in Medical Oncology. 2013;30(3).
doi:10.1007/s12032-013-0682-9 .

Davidović, Radoslav S., Sopta, Jelena, Mandušić, Vesna, Krajnović, Milena M., Stanojevic, Maja, Tulic, Goran, Dimitrijević, Bogomir B., "p14(ARF) methylation is a common event in the pathogenesis and progression of myxoid and pleomorphic liposarcoma" in Medical Oncology, 30, no. 3 (2013),
https://doi.org/10.1007/s12032-013-0682-9 . .

TY  - CONF
AU  - Sopta, Josipa
AU  - Davidović, Radoslav S.
AU  - Kovačević, Relja Z.
AU  - Lujić, Nenad
AU  - Ristić, Dalibor, V
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2742
C3  - European Journal of Cancer
T1  - p14ARF methylation is a common event in the pathogenesis and progression of mixoid and pleomorphic liposarcoma
VL  - 49
SP  - S15
EP  - S16
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2742
ER  - 

@conference{
author = "Sopta, Josipa and Davidović, Radoslav S. and Kovačević, Relja Z. and Lujić, Nenad and Ristić, Dalibor, V",
year = "2013",
journal = "European Journal of Cancer",
title = "p14ARF methylation is a common event in the pathogenesis and progression of mixoid and pleomorphic liposarcoma",
volume = "49",
pages = "S15-S16",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2742"
}

Sopta, J., Davidović, R. S., Kovačević, R. Z., Lujić, N.,& Ristić, D. V.. (2013). p14ARF methylation is a common event in the pathogenesis and progression of mixoid and pleomorphic liposarcoma. in European Journal of Cancer, 49, S15-S16.
https://hdl.handle.net/21.15107/rcub_vinar_2742

Sopta J, Davidović RS, Kovačević RZ, Lujić N, Ristić DV. p14ARF methylation is a common event in the pathogenesis and progression of mixoid and pleomorphic liposarcoma. in European Journal of Cancer. 2013;49:S15-S16.
https://hdl.handle.net/21.15107/rcub_vinar_2742 .

Sopta, Josipa, Davidović, Radoslav S., Kovačević, Relja Z., Lujić, Nenad, Ristić, Dalibor, V, "p14ARF methylation is a common event in the pathogenesis and progression of mixoid and pleomorphic liposarcoma" in European Journal of Cancer, 49 (2013):S15-S16,
https://hdl.handle.net/21.15107/rcub_vinar_2742 .