@article{
author = "Avdeenko, Anatoly P. and Belova, Elena A. and Dašić, Predrag V. and Konovalova, Svetlana A. and Baklanova, Larisa V. and Krstić, Sanja S. and Milosavljević, Milutin M.",
year = "2017",
abstract = "Carotenoids are known for their lower environmental pollution, lower costs, shorter extraction time compared to regular extraction methods and higher extraction efficiency. Ultrasound has significant advantages in the extraction of a great number of biologically active compounds including carotenoids. Benefits of using ultrasound for extraction of various compounds are numerous and include effective mixing and micro-mixing, more efficient energy transfer, reduced thermal and concentration gradients, reduced temperature. The aim of this work was to investigate the influence of two-frequency ultrasound main parameters such as ultrasonic intensity, sonication time, and temperature. Application of two-frequency ultrasound (simultaneous influence of ultrasound with frequency of 20-46 kHz, intensity of 1.5-2.5 W/cm(2) and frequency of 1.0-2.0 MHz, intensity of 2.0- 3.0 W/cm(2)) raises the percentage of beta-carotene extraction from biomass of the fungus Blakeslea trispora from 90-92% up to 98-99% in comparison with one frequency ultrasound. The recommended oil temperature for the extraction process is 35 degrees C, because lower temperature leads to reduction of percentage of beta-carotene extraction, and higher temperature promotes unjustified increase of expenditure of energy.",
journal = "Hemijska industrija",
title = "Efficient two-frequency ultrasound extraction of beta-carotene from the fungus Blakeslea trispora",
volume = "71",
number = "4",
pages = "329-336",
doi = "10.2298/HEMIND151110043A"
}