TY  - JOUR
AU  - Dorontić, Slađana
AU  - Bonasera, Aurelio
AU  - Scopelliti, Michelangelo
AU  - Marković, Olivera
AU  - Verbić, Tatjana
AU  - Sredojević, Dušan
AU  - Ciasca, Gabriele
AU  - Di Santo, Riccardo
AU  - Mead, James L.
AU  - Budimir, Milica
AU  - Bajuk-Bogdanović, Danica
AU  - Mojsin, Marija
AU  - Pejić, Jelena
AU  - Stevanović, Milena
AU  - Jovanović, Svetlana
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13322
AB  - The widespread usage of the herbicide glyphosate calls for urgent action, aiming at the development of new, simple, low-cost, and eco-friendly detection approaches. In the last decade, investigation of graphene quantum dots (GQDs) as potential optical probes for various pollutants rapidly grew, thanks to their easy-manipulative structure, remarkable photoluminescence (PL) in the visible part of the spectrum, good dispersibility, biocompatibility, and non-toxicity, as well. Herein, a fast, simple, and environmentally friendly method for GQDs structural modification is presented. GQDs raw powder was exposed to γ- rays at three different doses (100, 200, and 300 kGy) in air, without any solvent or reagents. Irradiation of dots under such affordable conditions led to the additional incorporation of oxygen-containing moieties in the GQD structure. For the first time, oxygen-rich GQDs irradiated at a 300 kGy dose were successfully applied as direct turn-off PL probe for glyphosate detection. The high coefficient of determination (R-squared (R2) = 0.99) and very low limit of detection (3.02 μmol L-1) reveal good linearity between the potential sensor and analyte, as well as sensitivity. Glyphosate was successfully detected in celery samples, with a recovery value of 107 ± 0.85%. To evaluate the biological safety of the proposed sensing probe, [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) and the hemolysis assays were performed. Obtained results show that irradiated and non-irradiated GQDs did not cause the death of MRC-5 cells, and hemolysis of erythrocytes. The obtained results demonstrate that GQDs irradiated in an air medium can be potentially applied for glyphosate detection.
T2  - Journal of Environmental Chemical Engineering
T1  - High-performing structural optimization of graphene quantum dots as glyphosate herbicide photoluminescent probes: real case studies and mechanism insights
VL  - 12
IS  - 4
SP  - 113193
DO  - 10.1016/j.jece.2024.113193
ER  - 

@article{
author = "Dorontić, Slađana and Bonasera, Aurelio and Scopelliti, Michelangelo and Marković, Olivera and Verbić, Tatjana and Sredojević, Dušan and Ciasca, Gabriele and Di Santo, Riccardo and Mead, James L. and Budimir, Milica and Bajuk-Bogdanović, Danica and Mojsin, Marija and Pejić, Jelena and Stevanović, Milena and Jovanović, Svetlana",
year = "2024",
abstract = "The widespread usage of the herbicide glyphosate calls for urgent action, aiming at the development of new, simple, low-cost, and eco-friendly detection approaches. In the last decade, investigation of graphene quantum dots (GQDs) as potential optical probes for various pollutants rapidly grew, thanks to their easy-manipulative structure, remarkable photoluminescence (PL) in the visible part of the spectrum, good dispersibility, biocompatibility, and non-toxicity, as well. Herein, a fast, simple, and environmentally friendly method for GQDs structural modification is presented. GQDs raw powder was exposed to γ- rays at three different doses (100, 200, and 300 kGy) in air, without any solvent or reagents. Irradiation of dots under such affordable conditions led to the additional incorporation of oxygen-containing moieties in the GQD structure. For the first time, oxygen-rich GQDs irradiated at a 300 kGy dose were successfully applied as direct turn-off PL probe for glyphosate detection. The high coefficient of determination (R-squared (R2) = 0.99) and very low limit of detection (3.02 μmol L-1) reveal good linearity between the potential sensor and analyte, as well as sensitivity. Glyphosate was successfully detected in celery samples, with a recovery value of 107 ± 0.85%. To evaluate the biological safety of the proposed sensing probe, [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) and the hemolysis assays were performed. Obtained results show that irradiated and non-irradiated GQDs did not cause the death of MRC-5 cells, and hemolysis of erythrocytes. The obtained results demonstrate that GQDs irradiated in an air medium can be potentially applied for glyphosate detection.",
journal = "Journal of Environmental Chemical Engineering",
title = "High-performing structural optimization of graphene quantum dots as glyphosate herbicide photoluminescent probes: real case studies and mechanism insights",
volume = "12",
number = "4",
pages = "113193",
doi = "10.1016/j.jece.2024.113193"
}

Dorontić, S., Bonasera, A., Scopelliti, M., Marković, O., Verbić, T., Sredojević, D., Ciasca, G., Di Santo, R., Mead, J. L., Budimir, M., Bajuk-Bogdanović, D., Mojsin, M., Pejić, J., Stevanović, M.,& Jovanović, S.. (2024). High-performing structural optimization of graphene quantum dots as glyphosate herbicide photoluminescent probes: real case studies and mechanism insights. in Journal of Environmental Chemical Engineering, 12(4), 113193.
https://doi.org/10.1016/j.jece.2024.113193

Dorontić S, Bonasera A, Scopelliti M, Marković O, Verbić T, Sredojević D, Ciasca G, Di Santo R, Mead JL, Budimir M, Bajuk-Bogdanović D, Mojsin M, Pejić J, Stevanović M, Jovanović S. High-performing structural optimization of graphene quantum dots as glyphosate herbicide photoluminescent probes: real case studies and mechanism insights. in Journal of Environmental Chemical Engineering. 2024;12(4):113193.
doi:10.1016/j.jece.2024.113193 .

Dorontić, Slađana, Bonasera, Aurelio, Scopelliti, Michelangelo, Marković, Olivera, Verbić, Tatjana, Sredojević, Dušan, Ciasca, Gabriele, Di Santo, Riccardo, Mead, James L., Budimir, Milica, Bajuk-Bogdanović, Danica, Mojsin, Marija, Pejić, Jelena, Stevanović, Milena, Jovanović, Svetlana, "High-performing structural optimization of graphene quantum dots as glyphosate herbicide photoluminescent probes: real case studies and mechanism insights" in Journal of Environmental Chemical Engineering, 12, no. 4 (2024):113193,
https://doi.org/10.1016/j.jece.2024.113193 . .

TY  - JOUR
AU  - Milenković, Mila
AU  - Ciasca, Gabriele
AU  - Bonasera, Aurelio
AU  - Scopelliti, Michelangelo
AU  - Marković, Olivera
AU  - Verbić, Tatjana
AU  - Todorović Marković, Biljana
AU  - Jovanović, Svetlana
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12238
AB  - The widespread abuse of traditional antibiotics has led to a global rise in antibiotic-resistant bacteria, which give in return unprecedented health risks. Therefore, there is a large and urgent need for the development of new, smart antibacterial agents able to efficiently kill or inhibit bacterial growth. In this study, we investigated the antibacterial activity of S, N-doped Graphene Quantum Dots (GQDs) as a light-triggered antibacterial agent. Gamma irradiation was employed as a tool to achieve one-step modification of GQDs in the presence of L cysteine amino acid as a source of heteroatoms. X-ray Photoelectron Spectroscopy (XPS), nuclear magnetic resonance (NMR), and zeta potential measurements provided the necessary data to clarify the structure of modified dots and verify the introduction of both S- and N-atoms in GQDs structure, but also severe changes in the aromatic, sp2 domains. Namely, γ-irradiation caused a bonding of S atoms in 1.14 at.% mainly as thiol groups, and N in 1.81 at.% as amino groups, but sp2 contribution in GQD structure was lowered from 63.00 to 4.86 at.%, as measured in dots irradiated at a dose of 200 kGy. Fluorescence quenching measurements showed that L-cysteine-modified dots are able to bind to human serum albumin. The antibacterial activity of GQDs combined with 1 and 6 h of blue light (470 nm) irradiation was tested against 8 bacterial strains. GQD-cys-25 sample provided the best results, with minimum inhibitory concentration (MIC) as low as 125 μg/mL against S. aureus, E. faecalis, and E. coli after only 1 h of blue light exposure.
T2  - Journal of Photochemistry & Photobiology, B: Biology
T1  - Blue-light-driven photoactivity of L-cysteine-modified graphene quantum  dots and their antibacterial effects
VL  - 250
SP  - 112818
DO  - 10.1016/j.jphotobiol.2023.112818
ER  - 

@article{
author = "Milenković, Mila and Ciasca, Gabriele and Bonasera, Aurelio and Scopelliti, Michelangelo and Marković, Olivera and Verbić, Tatjana and Todorović Marković, Biljana and Jovanović, Svetlana",
year = "2024",
abstract = "The widespread abuse of traditional antibiotics has led to a global rise in antibiotic-resistant bacteria, which give in return unprecedented health risks. Therefore, there is a large and urgent need for the development of new, smart antibacterial agents able to efficiently kill or inhibit bacterial growth. In this study, we investigated the antibacterial activity of S, N-doped Graphene Quantum Dots (GQDs) as a light-triggered antibacterial agent. Gamma irradiation was employed as a tool to achieve one-step modification of GQDs in the presence of L cysteine amino acid as a source of heteroatoms. X-ray Photoelectron Spectroscopy (XPS), nuclear magnetic resonance (NMR), and zeta potential measurements provided the necessary data to clarify the structure of modified dots and verify the introduction of both S- and N-atoms in GQDs structure, but also severe changes in the aromatic, sp2 domains. Namely, γ-irradiation caused a bonding of S atoms in 1.14 at.% mainly as thiol groups, and N in 1.81 at.% as amino groups, but sp2 contribution in GQD structure was lowered from 63.00 to 4.86 at.%, as measured in dots irradiated at a dose of 200 kGy. Fluorescence quenching measurements showed that L-cysteine-modified dots are able to bind to human serum albumin. The antibacterial activity of GQDs combined with 1 and 6 h of blue light (470 nm) irradiation was tested against 8 bacterial strains. GQD-cys-25 sample provided the best results, with minimum inhibitory concentration (MIC) as low as 125 μg/mL against S. aureus, E. faecalis, and E. coli after only 1 h of blue light exposure.",
journal = "Journal of Photochemistry & Photobiology, B: Biology",
title = "Blue-light-driven photoactivity of L-cysteine-modified graphene quantum  dots and their antibacterial effects",
volume = "250",
pages = "112818",
doi = "10.1016/j.jphotobiol.2023.112818"
}

Milenković, M., Ciasca, G., Bonasera, A., Scopelliti, M., Marković, O., Verbić, T., Todorović Marković, B.,& Jovanović, S.. (2024). Blue-light-driven photoactivity of L-cysteine-modified graphene quantum  dots and their antibacterial effects. in Journal of Photochemistry & Photobiology, B: Biology, 250, 112818.
https://doi.org/10.1016/j.jphotobiol.2023.112818

Milenković M, Ciasca G, Bonasera A, Scopelliti M, Marković O, Verbić T, Todorović Marković B, Jovanović S. Blue-light-driven photoactivity of L-cysteine-modified graphene quantum  dots and their antibacterial effects. in Journal of Photochemistry & Photobiology, B: Biology. 2024;250:112818.
doi:10.1016/j.jphotobiol.2023.112818 .

Milenković, Mila, Ciasca, Gabriele, Bonasera, Aurelio, Scopelliti, Michelangelo, Marković, Olivera, Verbić, Tatjana, Todorović Marković, Biljana, Jovanović, Svetlana, "Blue-light-driven photoactivity of L-cysteine-modified graphene quantum  dots and their antibacterial effects" in Journal of Photochemistry & Photobiology, B: Biology, 250 (2024):112818,
https://doi.org/10.1016/j.jphotobiol.2023.112818 . .