TY  - JOUR
AU  - Mladenovic, Dusan
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Radosavljevic, Tatjana
AU  - Rašić-Marković, Aleksandra
AU  - Hrnčić, Dragan
AU  - Macut, Djuro
AU  - Stanojlović, Olivera
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4696
AB  - Thioacetamide (TAA) exerts hepatotoxic, neurotoxic and carcinogenic effects. The aim of our study was to investigate the effects of TAA on lipid peroxidation and catalase activity in various rat brain regions. Male Wistar rats were divided into following groups: 1. control, saline-treated; 2. thioacetamide-treated groups, TAA(300) (300 mg/kg), TAA(600) (600 mg/kg) and TAA(900) (900 mg/kg). Daily dose of TAA (300 mg/kg) was administered intraperitoneally once (TAA(300)), twice (TAA(600)) and three times (TAA(900)) in consecutive days. Brain samples were collected 24 h after the last dose of TAA and malondialdehyde (MDA) level and catalase activity were determined in cortex, brainstem and hippocampus. MDA level was significantly increased while catalase activity was significantly lower in all brain regions in TAA(900) group in comparison with control group. In TAA(600) MDA level was increased in the brainstem and cortex when compared to control (p LT 0.01). The same dose of TAA(600) mg/kg induced a significant decline in catalase activity in the brainstem and cortex and an increase in its activity in the hippocampus when compared to control (p LT 0.01). In TAA(300) an increase in MDA level was evident only in the brainstem. Catalase activity was significantly higher in the cortex and hippocampus in TAA(300) group in comparison with control (p LT 0.01). Based on these results, it may be concluded that various rat brain regions have different sensitivity to TAA-induced lipid peroxidation with hippocampus being less sensitive than cerebral cortex and brainstem.
T2  - Medicinal Chemistry
T1  - Different Sensitivity of Various Brain Structures to Thioacetamide-Induced Lipid Peroxidation
VL  - 8
IS  - 1
SP  - 52
EP  - 58
DO  - 10.2174/157340612799278603
ER  - 

@article{
author = "Mladenovic, Dusan and Krstić, Danijela Z. and Čolović, Mirjana B. and Radosavljevic, Tatjana and Rašić-Marković, Aleksandra and Hrnčić, Dragan and Macut, Djuro and Stanojlović, Olivera",
year = "2012",
abstract = "Thioacetamide (TAA) exerts hepatotoxic, neurotoxic and carcinogenic effects. The aim of our study was to investigate the effects of TAA on lipid peroxidation and catalase activity in various rat brain regions. Male Wistar rats were divided into following groups: 1. control, saline-treated; 2. thioacetamide-treated groups, TAA(300) (300 mg/kg), TAA(600) (600 mg/kg) and TAA(900) (900 mg/kg). Daily dose of TAA (300 mg/kg) was administered intraperitoneally once (TAA(300)), twice (TAA(600)) and three times (TAA(900)) in consecutive days. Brain samples were collected 24 h after the last dose of TAA and malondialdehyde (MDA) level and catalase activity were determined in cortex, brainstem and hippocampus. MDA level was significantly increased while catalase activity was significantly lower in all brain regions in TAA(900) group in comparison with control group. In TAA(600) MDA level was increased in the brainstem and cortex when compared to control (p LT 0.01). The same dose of TAA(600) mg/kg induced a significant decline in catalase activity in the brainstem and cortex and an increase in its activity in the hippocampus when compared to control (p LT 0.01). In TAA(300) an increase in MDA level was evident only in the brainstem. Catalase activity was significantly higher in the cortex and hippocampus in TAA(300) group in comparison with control (p LT 0.01). Based on these results, it may be concluded that various rat brain regions have different sensitivity to TAA-induced lipid peroxidation with hippocampus being less sensitive than cerebral cortex and brainstem.",
journal = "Medicinal Chemistry",
title = "Different Sensitivity of Various Brain Structures to Thioacetamide-Induced Lipid Peroxidation",
volume = "8",
number = "1",
pages = "52-58",
doi = "10.2174/157340612799278603"
}

Mladenovic, D., Krstić, D. Z., Čolović, M. B., Radosavljevic, T., Rašić-Marković, A., Hrnčić, D., Macut, D.,& Stanojlović, O.. (2012). Different Sensitivity of Various Brain Structures to Thioacetamide-Induced Lipid Peroxidation. in Medicinal Chemistry, 8(1), 52-58.
https://doi.org/10.2174/157340612799278603

Mladenovic D, Krstić DZ, Čolović MB, Radosavljevic T, Rašić-Marković A, Hrnčić D, Macut D, Stanojlović O. Different Sensitivity of Various Brain Structures to Thioacetamide-Induced Lipid Peroxidation. in Medicinal Chemistry. 2012;8(1):52-58.
doi:10.2174/157340612799278603 .

Mladenovic, Dusan, Krstić, Danijela Z., Čolović, Mirjana B., Radosavljevic, Tatjana, Rašić-Marković, Aleksandra, Hrnčić, Dragan, Macut, Djuro, Stanojlović, Olivera, "Different Sensitivity of Various Brain Structures to Thioacetamide-Induced Lipid Peroxidation" in Medicinal Chemistry, 8, no. 1 (2012):52-58,
https://doi.org/10.2174/157340612799278603 . .

TY  - JOUR
AU  - Rašić-Marković, Aleksandra
AU  - Stanojlović, Olivera
AU  - Hrnčić, Dragan
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Šušić, Veselinka
AU  - Radosavljevic, Tatjana
AU  - Đurić, Dragan M.
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3709
AB  - Hyperhomocysteinemia is associated with various pathologies including cardiovascular disease, stroke, and cognitive dysfunctions. Systemic administration of homocysteine can trigger seizures in animals, and patients with homocystinuria suffer from epileptic seizures. Available data suggest that homocysteine can be harmful to human cells because of its metabolic conversion to homocysteine thiolactone, a reactive thioester. A number of reports have demonstrated a reduction of Na+/K+-ATPase activity in cerebral ischemia, epilepsy and neurodegeneration possibly associated with excitotoxic mechanisms. The aim of this study was to examine the in vivo effects of d,l-homocysteine and d,l-homocysteine thiolactone on Na+/K+- and Mg2+-ATPase activities in erythrocyte (RBC), brain cortex, hippocampus, and brain stem of adult male rats. Our results demonstrate a moderate inhibition of rat hippocampal Na+/K+-ATPase activity by d,l-homocysteine, which however expressed no effect on the activity of this enzyme in the cortex and brain stem. In contrast,d,l-homocysteine thiolactone strongly inhibited Na+/K+-ATPase activity in cortex, hippocampus and brain stem of rats. RBC Na+/K+-ATPase and Mg2+-ATPase activities were not affected by d,l-homocysteine, while d,l-homocysteine thiolactone inhibited only Na+/K+-ATPase activity. This study results show that homocysteine thiolactone significantly inhibits Na+/K+-ATPase activity in the cortex, hippocampus, and brain stem, which may contribute at least in part to the understanding of excitotoxic and convulsive properties of this substance.
T2  - Molecular and Cellular Biochemistry
T1  - The activity of erythrocyte and brain Na+/K+ and Mg2+-ATPases in rats subjected to acute homocysteine and homocysteine thiolactone administration
VL  - 327
IS  - 1-2
SP  - 39
EP  - 45
DO  - 10.1007/s11010-009-0040-6
ER  - 

@article{
author = "Rašić-Marković, Aleksandra and Stanojlović, Olivera and Hrnčić, Dragan and Krstić, Danijela Z. and Čolović, Mirjana B. and Šušić, Veselinka and Radosavljevic, Tatjana and Đurić, Dragan M.",
year = "2009",
abstract = "Hyperhomocysteinemia is associated with various pathologies including cardiovascular disease, stroke, and cognitive dysfunctions. Systemic administration of homocysteine can trigger seizures in animals, and patients with homocystinuria suffer from epileptic seizures. Available data suggest that homocysteine can be harmful to human cells because of its metabolic conversion to homocysteine thiolactone, a reactive thioester. A number of reports have demonstrated a reduction of Na+/K+-ATPase activity in cerebral ischemia, epilepsy and neurodegeneration possibly associated with excitotoxic mechanisms. The aim of this study was to examine the in vivo effects of d,l-homocysteine and d,l-homocysteine thiolactone on Na+/K+- and Mg2+-ATPase activities in erythrocyte (RBC), brain cortex, hippocampus, and brain stem of adult male rats. Our results demonstrate a moderate inhibition of rat hippocampal Na+/K+-ATPase activity by d,l-homocysteine, which however expressed no effect on the activity of this enzyme in the cortex and brain stem. In contrast,d,l-homocysteine thiolactone strongly inhibited Na+/K+-ATPase activity in cortex, hippocampus and brain stem of rats. RBC Na+/K+-ATPase and Mg2+-ATPase activities were not affected by d,l-homocysteine, while d,l-homocysteine thiolactone inhibited only Na+/K+-ATPase activity. This study results show that homocysteine thiolactone significantly inhibits Na+/K+-ATPase activity in the cortex, hippocampus, and brain stem, which may contribute at least in part to the understanding of excitotoxic and convulsive properties of this substance.",
journal = "Molecular and Cellular Biochemistry",
title = "The activity of erythrocyte and brain Na+/K+ and Mg2+-ATPases in rats subjected to acute homocysteine and homocysteine thiolactone administration",
volume = "327",
number = "1-2",
pages = "39-45",
doi = "10.1007/s11010-009-0040-6"
}

Rašić-Marković, A., Stanojlović, O., Hrnčić, D., Krstić, D. Z., Čolović, M. B., Šušić, V., Radosavljevic, T.,& Đurić, D. M.. (2009). The activity of erythrocyte and brain Na+/K+ and Mg2+-ATPases in rats subjected to acute homocysteine and homocysteine thiolactone administration. in Molecular and Cellular Biochemistry, 327(1-2), 39-45.
https://doi.org/10.1007/s11010-009-0040-6

Rašić-Marković A, Stanojlović O, Hrnčić D, Krstić DZ, Čolović MB, Šušić V, Radosavljevic T, Đurić DM. The activity of erythrocyte and brain Na+/K+ and Mg2+-ATPases in rats subjected to acute homocysteine and homocysteine thiolactone administration. in Molecular and Cellular Biochemistry. 2009;327(1-2):39-45.
doi:10.1007/s11010-009-0040-6 .

Rašić-Marković, Aleksandra, Stanojlović, Olivera, Hrnčić, Dragan, Krstić, Danijela Z., Čolović, Mirjana B., Šušić, Veselinka, Radosavljevic, Tatjana, Đurić, Dragan M., "The activity of erythrocyte and brain Na+/K+ and Mg2+-ATPases in rats subjected to acute homocysteine and homocysteine thiolactone administration" in Molecular and Cellular Biochemistry, 327, no. 1-2 (2009):39-45,
https://doi.org/10.1007/s11010-009-0040-6 . .