TY  - CONF
AU  - Guševac Stojanović, Ivana
AU  - Drakulić, Dunja R.
AU  - Stanojlović, Miloš R.
AU  - Grković, Ivana
AU  - Martinović, Jelena
AU  - Mitrović, Nataša Lj.
AU  - Zarić, Marina
AU  - Veljković, Filip M.
AU  - Horvat, Anica
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9210
AB  - Reduction  of  oxygen  and  glucose  supply  to  the  brain  due  to  diminished cerebral  blood  flow  leads  to  damage  of  tissue  which  in  experimental conditions  can  be  mimicked  by  permanent  ligation  of  common  carotid arteries  (2VO).  Besides  numerous  genomic  and  non-genomic  processes, cerebral  ischemia  enhances  expression  of  ecto-5'-nucleotidase  (eN),  a  main enzyme  in  the  central  nervous  system  that  produces  potent  neuromodulator and neuroprotector, adenosine. Since progesterone (P), a potent sex steroid, is recognized as neuroprotective, aim of this study was to examine whether repeated  low-dose  P  treatment  is  capable  to  induce  changes  in  activity  and protein expression of eN, at rat cortical membrane fraction following 2VO. Obtained  results  indicate  that  P  modulates  investigated  parameters  and through  stimulation  of  adenosine  generation  might  promote  cytoprotection in ischemic brain.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry
T1  - Progesterone upregulates activity and protein expression of efecto-5'-nucleotidase in ischemic brain of male wister rats
SP  - 455
EP  - 458
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9210
ER  - 

@conference{
author = "Guševac Stojanović, Ivana and Drakulić, Dunja R. and Stanojlović, Miloš R. and Grković, Ivana and Martinović, Jelena and Mitrović, Nataša Lj. and Zarić, Marina and Veljković, Filip M. and Horvat, Anica",
year = "2016",
abstract = "Reduction  of  oxygen  and  glucose  supply  to  the  brain  due  to  diminished cerebral  blood  flow  leads  to  damage  of  tissue  which  in  experimental conditions  can  be  mimicked  by  permanent  ligation  of  common  carotid arteries  (2VO).  Besides  numerous  genomic  and  non-genomic  processes, cerebral  ischemia  enhances  expression  of  ecto-5'-nucleotidase  (eN),  a  main enzyme  in  the  central  nervous  system  that  produces  potent  neuromodulator and neuroprotector, adenosine. Since progesterone (P), a potent sex steroid, is recognized as neuroprotective, aim of this study was to examine whether repeated  low-dose  P  treatment  is  capable  to  induce  changes  in  activity  and protein expression of eN, at rat cortical membrane fraction following 2VO. Obtained  results  indicate  that  P  modulates  investigated  parameters  and through  stimulation  of  adenosine  generation  might  promote  cytoprotection in ischemic brain.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry",
title = "Progesterone upregulates activity and protein expression of efecto-5'-nucleotidase in ischemic brain of male wister rats",
pages = "455-458",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9210"
}

Guševac Stojanović, I., Drakulić, D. R., Stanojlović, M. R., Grković, I., Martinović, J., Mitrović, N. Lj., Zarić, M., Veljković, F. M.,& Horvat, A.. (2016). Progesterone upregulates activity and protein expression of efecto-5'-nucleotidase in ischemic brain of male wister rats. in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 455-458.
https://hdl.handle.net/21.15107/rcub_vinar_9210

Guševac Stojanović I, Drakulić DR, Stanojlović MR, Grković I, Martinović J, Mitrović NL, Zarić M, Veljković FM, Horvat A. Progesterone upregulates activity and protein expression of efecto-5'-nucleotidase in ischemic brain of male wister rats. in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry. 2016;:455-458.
https://hdl.handle.net/21.15107/rcub_vinar_9210 .

Guševac Stojanović, Ivana, Drakulić, Dunja R., Stanojlović, Miloš R., Grković, Ivana, Martinović, Jelena, Mitrović, Nataša Lj., Zarić, Marina, Veljković, Filip M., Horvat, Anica, "Progesterone upregulates activity and protein expression of efecto-5'-nucleotidase in ischemic brain of male wister rats" in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry (2016):455-458,
https://hdl.handle.net/21.15107/rcub_vinar_9210 .

TY  - JOUR
AU  - Grković, Ivana
AU  - Bjelobaba, Ivana
AU  - Mitrović, Nataša Lj.
AU  - Lavrnja, Irena
AU  - Drakulić, Dunja R.
AU  - Martinović, Jelena
AU  - Stanojlović, Miloš R.
AU  - Horvat, Anica
AU  - Nedeljković, Nadežda
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1289
AB  - Nucleoside triphosphate diphosphohydrolase3 (NTPDase3) is membrane-bound ecto-enzyme which hydrolyzes extracellular ATP, thus modulating the function of purinergic receptors and the pattern of purinergic signaling. Here we analyzed the developmental expression of NTPDase3 in female hypothalamus, cerebral cortex and hippocampal formation at different postnatal ages (PD7-PD90) by qRT-PCR and immunohistochemistry. In hypothalamus and hippocampus, a similar developmental profile was seen: NTPDase3 gene expression was stable during postnatal development and increased in adults. In the cortex, upregulation of NTPDase3 mRNA expression was seen at PD15 and further increase was evidenced in adults. Immunohistochemical analysis at PD7 revealed faint neuronal NTPDase3 localization in a dorsal hypothalamus. The immunoreactivity (ir) gradually increased in PD15 and PD20, in clusters of cells in the lateral, ventral and dorsomedial hypothalamus. Furthermore, in PD20 animals, NTPDase3-ir was detected on short fibers in the posterior hypothalamic area, while in PD30 the fibers appeared progressively longer and markedly varicose. In adults, the strongest NTPDase3-ir was observed in collections of cells in dorsomedial hypothalamic nucleus, dorsal and lateral hypothalamus and in several thalamic areas, whereas the varicose fibers traversed entire diencephalon, particularly paraventricular thalamic nucleus, ventromedial and dorsomedial hypothalamic nuclei, the arcuate nucleus and the prefornical part of the lateral hypothalamus. The presumably ascending NTPDase3-ir fibers were first observed in PD20; their density and the varicose appearance increased until the adulthood. Prominent enhancement of NTPDase3-ir in the hypothalamus coincides with age when animals acquire diurnal rhythms of sleeping and feeding, supporting the hypothesis that this enzyme may be involved in regulation of homeostatic functions. (C) 2016 Elsevier B.V. All rights reserved.
T2  - Journal of Chemical Neuroanatomy
T1  - Expression of ecto-nucleoside triphosphate diphosphohydrolase3 (NTPDase3) in the female rat brain during postnatal development
VL  - 77
SP  - 10
EP  - 18
DO  - 10.1016/j.jchemneu.2016.04.001
ER  - 

@article{
author = "Grković, Ivana and Bjelobaba, Ivana and Mitrović, Nataša Lj. and Lavrnja, Irena and Drakulić, Dunja R. and Martinović, Jelena and Stanojlović, Miloš R. and Horvat, Anica and Nedeljković, Nadežda",
year = "2016",
abstract = "Nucleoside triphosphate diphosphohydrolase3 (NTPDase3) is membrane-bound ecto-enzyme which hydrolyzes extracellular ATP, thus modulating the function of purinergic receptors and the pattern of purinergic signaling. Here we analyzed the developmental expression of NTPDase3 in female hypothalamus, cerebral cortex and hippocampal formation at different postnatal ages (PD7-PD90) by qRT-PCR and immunohistochemistry. In hypothalamus and hippocampus, a similar developmental profile was seen: NTPDase3 gene expression was stable during postnatal development and increased in adults. In the cortex, upregulation of NTPDase3 mRNA expression was seen at PD15 and further increase was evidenced in adults. Immunohistochemical analysis at PD7 revealed faint neuronal NTPDase3 localization in a dorsal hypothalamus. The immunoreactivity (ir) gradually increased in PD15 and PD20, in clusters of cells in the lateral, ventral and dorsomedial hypothalamus. Furthermore, in PD20 animals, NTPDase3-ir was detected on short fibers in the posterior hypothalamic area, while in PD30 the fibers appeared progressively longer and markedly varicose. In adults, the strongest NTPDase3-ir was observed in collections of cells in dorsomedial hypothalamic nucleus, dorsal and lateral hypothalamus and in several thalamic areas, whereas the varicose fibers traversed entire diencephalon, particularly paraventricular thalamic nucleus, ventromedial and dorsomedial hypothalamic nuclei, the arcuate nucleus and the prefornical part of the lateral hypothalamus. The presumably ascending NTPDase3-ir fibers were first observed in PD20; their density and the varicose appearance increased until the adulthood. Prominent enhancement of NTPDase3-ir in the hypothalamus coincides with age when animals acquire diurnal rhythms of sleeping and feeding, supporting the hypothesis that this enzyme may be involved in regulation of homeostatic functions. (C) 2016 Elsevier B.V. All rights reserved.",
journal = "Journal of Chemical Neuroanatomy",
title = "Expression of ecto-nucleoside triphosphate diphosphohydrolase3 (NTPDase3) in the female rat brain during postnatal development",
volume = "77",
pages = "10-18",
doi = "10.1016/j.jchemneu.2016.04.001"
}

Grković, I., Bjelobaba, I., Mitrović, N. Lj., Lavrnja, I., Drakulić, D. R., Martinović, J., Stanojlović, M. R., Horvat, A.,& Nedeljković, N.. (2016). Expression of ecto-nucleoside triphosphate diphosphohydrolase3 (NTPDase3) in the female rat brain during postnatal development. in Journal of Chemical Neuroanatomy, 77, 10-18.
https://doi.org/10.1016/j.jchemneu.2016.04.001

Grković I, Bjelobaba I, Mitrović NL, Lavrnja I, Drakulić DR, Martinović J, Stanojlović MR, Horvat A, Nedeljković N. Expression of ecto-nucleoside triphosphate diphosphohydrolase3 (NTPDase3) in the female rat brain during postnatal development. in Journal of Chemical Neuroanatomy. 2016;77:10-18.
doi:10.1016/j.jchemneu.2016.04.001 .

Grković, Ivana, Bjelobaba, Ivana, Mitrović, Nataša Lj., Lavrnja, Irena, Drakulić, Dunja R., Martinović, Jelena, Stanojlović, Miloš R., Horvat, Anica, Nedeljković, Nadežda, "Expression of ecto-nucleoside triphosphate diphosphohydrolase3 (NTPDase3) in the female rat brain during postnatal development" in Journal of Chemical Neuroanatomy, 77 (2016):10-18,
https://doi.org/10.1016/j.jchemneu.2016.04.001 . .

TY  - JOUR
AU  - Mitrović, Nataša Lj.
AU  - Zarić, Marina
AU  - Drakulić, Dunja R.
AU  - Martinović, Jelena
AU  - Stanojlović, Miloš R.
AU  - Sevigny, Jean
AU  - Horvat, Anica
AU  - Nedeljković, Nadežda
AU  - Grković, Ivana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1022
AB  - 17 beta-Estradiol (E2) crucially affects several processes in the hippocampus of both sexes. E2 acts upon estradiol receptors ER alpha and ER beta, influencing target gene expression and/or modulates intracellular signaling cascades. Another potent modulator of hippocampal function is nucleoside adenosine, the final product of ectonucleoti-dase cascade, enzymes which hydrolyze extracellular ATP to adenosine. The last and rate-limiting step of the hydrolysis is catalyzed by membrane-bound ecto-50-nucleotidase (eN). Previous findings obtained on adenosine metabolism in brain suggest that eN may be modulated by ovarian steroids. Therefore, the present study reports that the activity and protein abundance of membrane-bound eN fluctuates across the estrus cycle in the hippocampal synaptosomes of female rats. Further, we analyzed the role of E2 and its intracellular receptors on the expression of eN in ovariectomized females. We found that E2 upregulated eN activity and protein abundance in the hippocampal synaptosomes. Application of nonspecific ER antagonist, ICI 182,780 and selective ERa and ERb agonists, PPT and DPN, respectively, demonstrated the involvement of both receptor subtypes in observed actions. Selective ERa receptor agonist, PPT, induced upregulation of both the protein level and activity of eN, while application of selective ERb receptor agonist, DPN, increased only the activity of eN. In both cases, E2 entered into the intracellular compartment and activated ER(s), which was demonstrated by membrane impermeable E2-BSA conjugate. Together these results imply that E2-induced effects on connectivity and functional properties of the hippocampal synapses may be in part mediated through observed effect on eN. (C) 2016 IBRO. Published by Elsevier Ltd. All rights reserved.
PB  - Elsevier
T2  - Neuroscience
T1  - 17 beta-ESTRADIOL UPREGULATES ECTO-5 -NUCLEOTIDASE (CD73) IN HIPPOCAMPAL SYNAPTOSOMES OF FEMALE RATS THROUGH ACTION MEDIATED BY ESTROGEN RECEPTOR-alpha AND -beta
VL  - 324
SP  - 286
EP  - 296
DO  - 10.1016/j.neuroscience.2016.03.022
ER  - 

@article{
author = "Mitrović, Nataša Lj. and Zarić, Marina and Drakulić, Dunja R. and Martinović, Jelena and Stanojlović, Miloš R. and Sevigny, Jean and Horvat, Anica and Nedeljković, Nadežda and Grković, Ivana",
year = "2016",
abstract = "17 beta-Estradiol (E2) crucially affects several processes in the hippocampus of both sexes. E2 acts upon estradiol receptors ER alpha and ER beta, influencing target gene expression and/or modulates intracellular signaling cascades. Another potent modulator of hippocampal function is nucleoside adenosine, the final product of ectonucleoti-dase cascade, enzymes which hydrolyze extracellular ATP to adenosine. The last and rate-limiting step of the hydrolysis is catalyzed by membrane-bound ecto-50-nucleotidase (eN). Previous findings obtained on adenosine metabolism in brain suggest that eN may be modulated by ovarian steroids. Therefore, the present study reports that the activity and protein abundance of membrane-bound eN fluctuates across the estrus cycle in the hippocampal synaptosomes of female rats. Further, we analyzed the role of E2 and its intracellular receptors on the expression of eN in ovariectomized females. We found that E2 upregulated eN activity and protein abundance in the hippocampal synaptosomes. Application of nonspecific ER antagonist, ICI 182,780 and selective ERa and ERb agonists, PPT and DPN, respectively, demonstrated the involvement of both receptor subtypes in observed actions. Selective ERa receptor agonist, PPT, induced upregulation of both the protein level and activity of eN, while application of selective ERb receptor agonist, DPN, increased only the activity of eN. In both cases, E2 entered into the intracellular compartment and activated ER(s), which was demonstrated by membrane impermeable E2-BSA conjugate. Together these results imply that E2-induced effects on connectivity and functional properties of the hippocampal synapses may be in part mediated through observed effect on eN. (C) 2016 IBRO. Published by Elsevier Ltd. All rights reserved.",
publisher = "Elsevier",
journal = "Neuroscience",
title = "17 beta-ESTRADIOL UPREGULATES ECTO-5 -NUCLEOTIDASE (CD73) IN HIPPOCAMPAL SYNAPTOSOMES OF FEMALE RATS THROUGH ACTION MEDIATED BY ESTROGEN RECEPTOR-alpha AND -beta",
volume = "324",
pages = "286-296",
doi = "10.1016/j.neuroscience.2016.03.022"
}

Mitrović, N. Lj., Zarić, M., Drakulić, D. R., Martinović, J., Stanojlović, M. R., Sevigny, J., Horvat, A., Nedeljković, N.,& Grković, I.. (2016). 17 beta-ESTRADIOL UPREGULATES ECTO-5 -NUCLEOTIDASE (CD73) IN HIPPOCAMPAL SYNAPTOSOMES OF FEMALE RATS THROUGH ACTION MEDIATED BY ESTROGEN RECEPTOR-alpha AND -beta. in Neuroscience
Elsevier., 324, 286-296.
https://doi.org/10.1016/j.neuroscience.2016.03.022

Mitrović NL, Zarić M, Drakulić DR, Martinović J, Stanojlović MR, Sevigny J, Horvat A, Nedeljković N, Grković I. 17 beta-ESTRADIOL UPREGULATES ECTO-5 -NUCLEOTIDASE (CD73) IN HIPPOCAMPAL SYNAPTOSOMES OF FEMALE RATS THROUGH ACTION MEDIATED BY ESTROGEN RECEPTOR-alpha AND -beta. in Neuroscience. 2016;324:286-296.
doi:10.1016/j.neuroscience.2016.03.022 .

Mitrović, Nataša Lj., Zarić, Marina, Drakulić, Dunja R., Martinović, Jelena, Stanojlović, Miloš R., Sevigny, Jean, Horvat, Anica, Nedeljković, Nadežda, Grković, Ivana, "17 beta-ESTRADIOL UPREGULATES ECTO-5 -NUCLEOTIDASE (CD73) IN HIPPOCAMPAL SYNAPTOSOMES OF FEMALE RATS THROUGH ACTION MEDIATED BY ESTROGEN RECEPTOR-alpha AND -beta" in Neuroscience, 324 (2016):286-296,
https://doi.org/10.1016/j.neuroscience.2016.03.022 . .

TY  - JOUR
AU  - Stanojlović, Miloš R.
AU  - Guševac, Ivana
AU  - Grković, Ivana
AU  - Mitrović, Nataša Lj.
AU  - Martinović, Jelena
AU  - Horvat, Anica
AU  - Drakulić, Dunja R.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1156
AB  - Although a substantial number of pre-clinical and experimental studies have investigated effects of 17 beta-estradiol, its precise molecular mechanism of action in the early state of chronic cerebral hypoperfusion remains controversial. The present study attempted to verify whether post-ischemic estradiol treatment (33.3 mu g/kg for seven consecutive days) affects previously reported number of hippocampal apoptotic cells and amount of DNA fragmentation characteristic for apoptosis as well as the expression of key elements within synaptosomal Akt and Erk signal transduction pathways (NF-kappa B, Bax, Bcl-2, cytochrome C, caspase 3, and PARP). Additionally, alterations of aforementioned molecules linked to protection in various neurodegenerative disorders were monitored in the cytosolic, mitochondrial, and nuclear fractions associating investigated kinases and NF-kappa B with gene expression of their downstream effectors-Bcl-2, Bax, and caspase 3. The results revealed that an initial increase in the number of apoptotic cells and amount of DNA fragmentation induced by chronic cerebral hypoperfusion was significantly reduced by 17 beta-estradiol. In synaptic regions, an altered profile with respect to the protein expression of Bcl-2 and phosphorylated Akt was detected, although the level of other examined proteins was not modified. In other investigated sub-cellular fractions, 17 beta-estradiol elicited phosphorylation and translocation of Akt and Erk along with modulation of the expression of their subsequent effectors. Our findings support the concept that repeated post-ischemic 17 beta-estradiol treatment attenuates neurodegeneration induced by chronic cerebral hypoperfusion in hippocampus through the activation of investigated kinases and regulation of their downstream molecules in sub-cellular manner indicating a time window and regime of its administration as a valid therapeutic intervention.
T2  - Cellular and Molecular Neurobiology
T1  - Repeated Estradiol Treatment Attenuates Chronic Cerebral Hypoperfusion-Induced Neurodegeneration in Rat Hippocampus
VL  - 36
IS  - 6
SP  - 989
EP  - 999
DO  - 10.1007/s10571-015-0289-0
ER  - 

@article{
author = "Stanojlović, Miloš R. and Guševac, Ivana and Grković, Ivana and Mitrović, Nataša Lj. and Martinović, Jelena and Horvat, Anica and Drakulić, Dunja R.",
year = "2016",
abstract = "Although a substantial number of pre-clinical and experimental studies have investigated effects of 17 beta-estradiol, its precise molecular mechanism of action in the early state of chronic cerebral hypoperfusion remains controversial. The present study attempted to verify whether post-ischemic estradiol treatment (33.3 mu g/kg for seven consecutive days) affects previously reported number of hippocampal apoptotic cells and amount of DNA fragmentation characteristic for apoptosis as well as the expression of key elements within synaptosomal Akt and Erk signal transduction pathways (NF-kappa B, Bax, Bcl-2, cytochrome C, caspase 3, and PARP). Additionally, alterations of aforementioned molecules linked to protection in various neurodegenerative disorders were monitored in the cytosolic, mitochondrial, and nuclear fractions associating investigated kinases and NF-kappa B with gene expression of their downstream effectors-Bcl-2, Bax, and caspase 3. The results revealed that an initial increase in the number of apoptotic cells and amount of DNA fragmentation induced by chronic cerebral hypoperfusion was significantly reduced by 17 beta-estradiol. In synaptic regions, an altered profile with respect to the protein expression of Bcl-2 and phosphorylated Akt was detected, although the level of other examined proteins was not modified. In other investigated sub-cellular fractions, 17 beta-estradiol elicited phosphorylation and translocation of Akt and Erk along with modulation of the expression of their subsequent effectors. Our findings support the concept that repeated post-ischemic 17 beta-estradiol treatment attenuates neurodegeneration induced by chronic cerebral hypoperfusion in hippocampus through the activation of investigated kinases and regulation of their downstream molecules in sub-cellular manner indicating a time window and regime of its administration as a valid therapeutic intervention.",
journal = "Cellular and Molecular Neurobiology",
title = "Repeated Estradiol Treatment Attenuates Chronic Cerebral Hypoperfusion-Induced Neurodegeneration in Rat Hippocampus",
volume = "36",
number = "6",
pages = "989-999",
doi = "10.1007/s10571-015-0289-0"
}

Stanojlović, M. R., Guševac, I., Grković, I., Mitrović, N. Lj., Martinović, J., Horvat, A.,& Drakulić, D. R.. (2016). Repeated Estradiol Treatment Attenuates Chronic Cerebral Hypoperfusion-Induced Neurodegeneration in Rat Hippocampus. in Cellular and Molecular Neurobiology, 36(6), 989-999.
https://doi.org/10.1007/s10571-015-0289-0

Stanojlović MR, Guševac I, Grković I, Mitrović NL, Martinović J, Horvat A, Drakulić DR. Repeated Estradiol Treatment Attenuates Chronic Cerebral Hypoperfusion-Induced Neurodegeneration in Rat Hippocampus. in Cellular and Molecular Neurobiology. 2016;36(6):989-999.
doi:10.1007/s10571-015-0289-0 .

Stanojlović, Miloš R., Guševac, Ivana, Grković, Ivana, Mitrović, Nataša Lj., Martinović, Jelena, Horvat, Anica, Drakulić, Dunja R., "Repeated Estradiol Treatment Attenuates Chronic Cerebral Hypoperfusion-Induced Neurodegeneration in Rat Hippocampus" in Cellular and Molecular Neurobiology, 36, no. 6 (2016):989-999,
https://doi.org/10.1007/s10571-015-0289-0 . .

TY  - JOUR
AU  - Mitrović, Nataša Lj.
AU  - Guševac, Ivana
AU  - Drakulić, Dunja R.
AU  - Stanojlović, Miloš R.
AU  - Martinović, Jelena
AU  - Sevigny, Jean
AU  - Horvat, Anica
AU  - Nedeljković, Nadežda
AU  - Grković, Ivana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1222
AB  - Ecto-5-nucleotidase (eN), a membrane rate-limiting enzyme of the purine catabolic pathway, catalyzes the conversion of AMP to adenosine involved in the regulation of many brain physiological and pathological processes. Since gender fundamentally determines hormonal milieu in the body and brain, it is reasonable to assume that sex differences in the activity of various signaling systems, including adenosine, may be generated by gonadal steroids. Thus, we examined expression of eN as a component of adenosine signaling system in the basal state in cerebral cortex and hippocampus of male and female rats at gene, protein and functional level, as well as in the state of gonadal hormone deprivation, induced by ovariectomy (OVX), whereas impact of steroid hormones was explored after repeated administration of 17 alpha-estradiol, 17 beta-estradiol and progesterone for seven consecutive days. Results showed regional and sex-related differences in basal eN activity level, with the highest AMP hydrolysis observed in the hippocampus of male rats. Furthermore, ovarian steroids do not contribute to basal gene eN expression or the activity in cortical and hippocampal region of female rats. However, protein eN expression was increased in OVX rats in both investigated region. Investigated exogenous steroids had no influence on eN expression in male brain, while in OVX females alterations in eN activity were induced. The observed effects in female rats were different between examined regions e.g. in cortex, applied treatments predominantly decreased whereas in hippocampus increased eN activity. Based on the presented results, eN exerts regional and sex-related response in basal state as well as after treatment with female gonadal hormones, however the exact mechanisms of sex steroids actions on eN remain unclear and should be fully explored. (C) 2016 Elsevier Inc. All rights reserved.
T2  - General and Comparative Endocrinology
T1  - Regional and sex-related differences in modulating effects of female sex steroids on ecto-5-nucleotidase expression in the rat cerebral cortex and hippocampus
VL  - 235
SP  - 100
EP  - 107
DO  - 10.1016/j.ygcen.2016.06.018
ER  - 

@article{
author = "Mitrović, Nataša Lj. and Guševac, Ivana and Drakulić, Dunja R. and Stanojlović, Miloš R. and Martinović, Jelena and Sevigny, Jean and Horvat, Anica and Nedeljković, Nadežda and Grković, Ivana",
year = "2016",
abstract = "Ecto-5-nucleotidase (eN), a membrane rate-limiting enzyme of the purine catabolic pathway, catalyzes the conversion of AMP to adenosine involved in the regulation of many brain physiological and pathological processes. Since gender fundamentally determines hormonal milieu in the body and brain, it is reasonable to assume that sex differences in the activity of various signaling systems, including adenosine, may be generated by gonadal steroids. Thus, we examined expression of eN as a component of adenosine signaling system in the basal state in cerebral cortex and hippocampus of male and female rats at gene, protein and functional level, as well as in the state of gonadal hormone deprivation, induced by ovariectomy (OVX), whereas impact of steroid hormones was explored after repeated administration of 17 alpha-estradiol, 17 beta-estradiol and progesterone for seven consecutive days. Results showed regional and sex-related differences in basal eN activity level, with the highest AMP hydrolysis observed in the hippocampus of male rats. Furthermore, ovarian steroids do not contribute to basal gene eN expression or the activity in cortical and hippocampal region of female rats. However, protein eN expression was increased in OVX rats in both investigated region. Investigated exogenous steroids had no influence on eN expression in male brain, while in OVX females alterations in eN activity were induced. The observed effects in female rats were different between examined regions e.g. in cortex, applied treatments predominantly decreased whereas in hippocampus increased eN activity. Based on the presented results, eN exerts regional and sex-related response in basal state as well as after treatment with female gonadal hormones, however the exact mechanisms of sex steroids actions on eN remain unclear and should be fully explored. (C) 2016 Elsevier Inc. All rights reserved.",
journal = "General and Comparative Endocrinology",
title = "Regional and sex-related differences in modulating effects of female sex steroids on ecto-5-nucleotidase expression in the rat cerebral cortex and hippocampus",
volume = "235",
pages = "100-107",
doi = "10.1016/j.ygcen.2016.06.018"
}

Mitrović, N. Lj., Guševac, I., Drakulić, D. R., Stanojlović, M. R., Martinović, J., Sevigny, J., Horvat, A., Nedeljković, N.,& Grković, I.. (2016). Regional and sex-related differences in modulating effects of female sex steroids on ecto-5-nucleotidase expression in the rat cerebral cortex and hippocampus. in General and Comparative Endocrinology, 235, 100-107.
https://doi.org/10.1016/j.ygcen.2016.06.018

Mitrović NL, Guševac I, Drakulić DR, Stanojlović MR, Martinović J, Sevigny J, Horvat A, Nedeljković N, Grković I. Regional and sex-related differences in modulating effects of female sex steroids on ecto-5-nucleotidase expression in the rat cerebral cortex and hippocampus. in General and Comparative Endocrinology. 2016;235:100-107.
doi:10.1016/j.ygcen.2016.06.018 .

Mitrović, Nataša Lj., Guševac, Ivana, Drakulić, Dunja R., Stanojlović, Miloš R., Martinović, Jelena, Sevigny, Jean, Horvat, Anica, Nedeljković, Nadežda, Grković, Ivana, "Regional and sex-related differences in modulating effects of female sex steroids on ecto-5-nucleotidase expression in the rat cerebral cortex and hippocampus" in General and Comparative Endocrinology, 235 (2016):100-107,
https://doi.org/10.1016/j.ygcen.2016.06.018 . .

TY  - JOUR
AU  - Drakulić, Dunja R.
AU  - Stanojlović, Miloš R.
AU  - Nedeljković, Nadežda
AU  - Grković, Ivana
AU  - Velickovic, Natasa
AU  - Guševac, Ivana
AU  - Mitrović, Nataša Lj.
AU  - Buzadzic, Ivana
AU  - Horvat, Anica
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/446
AB  - Although dexamethasone (DEX), a synthetic glucocorticoid receptor (GR) analog with profound effects on energy metabolism, immune system, and hypothalamic-pituitary-adrenal axis, is widely used therapeutically, its impact on the brain is poorly understood. The aim of the present study was to explore the effect of repeated low-dose DEX administration on the activity and expression of the ectonucleotidase enzymes which hydrolyze and therefore control extracellular ATP and adenosine concentrations in the synaptic cleft. Ectonucleotidases tested were ectonucleoside triphosphate diphosphohydrolase 1-3 (NTPDase1-3) and ecto-5-nucleotidase (eN), whereas the effects were evaluated in two brain areas that show different sensitivity to glucocorticoid action, hippocampus, and cerebral cortex. In the hippocampus, but not in cerebral cortex, modest level of neurodegenerative changes as well as increase in ATP, ADP, and AMP hydrolysis and upregulation of NTPDase1 and eN mRNA expression ensued under the influence of DEX. The observed pattern of ectonucleotidase activation, which creates tissue volume with enhanced capacity for adenosine formation, is the hallmark of the response after different insults to the brain.
T2  - Journal of Molecular Neuroscience
T1  - Upregulation of Nucleoside Triphosphate Diphosphohydrolase-1 and Ecto-5-Nucleotidase in Rat Hippocampus after Repeated Low-Dose Dexamethasone Administration
VL  - 55
IS  - 4
SP  - 959
EP  - 967
DO  - 10.1007/s12031-014-0452-y
ER  - 

@article{
author = "Drakulić, Dunja R. and Stanojlović, Miloš R. and Nedeljković, Nadežda and Grković, Ivana and Velickovic, Natasa and Guševac, Ivana and Mitrović, Nataša Lj. and Buzadzic, Ivana and Horvat, Anica",
year = "2015",
abstract = "Although dexamethasone (DEX), a synthetic glucocorticoid receptor (GR) analog with profound effects on energy metabolism, immune system, and hypothalamic-pituitary-adrenal axis, is widely used therapeutically, its impact on the brain is poorly understood. The aim of the present study was to explore the effect of repeated low-dose DEX administration on the activity and expression of the ectonucleotidase enzymes which hydrolyze and therefore control extracellular ATP and adenosine concentrations in the synaptic cleft. Ectonucleotidases tested were ectonucleoside triphosphate diphosphohydrolase 1-3 (NTPDase1-3) and ecto-5-nucleotidase (eN), whereas the effects were evaluated in two brain areas that show different sensitivity to glucocorticoid action, hippocampus, and cerebral cortex. In the hippocampus, but not in cerebral cortex, modest level of neurodegenerative changes as well as increase in ATP, ADP, and AMP hydrolysis and upregulation of NTPDase1 and eN mRNA expression ensued under the influence of DEX. The observed pattern of ectonucleotidase activation, which creates tissue volume with enhanced capacity for adenosine formation, is the hallmark of the response after different insults to the brain.",
journal = "Journal of Molecular Neuroscience",
title = "Upregulation of Nucleoside Triphosphate Diphosphohydrolase-1 and Ecto-5-Nucleotidase in Rat Hippocampus after Repeated Low-Dose Dexamethasone Administration",
volume = "55",
number = "4",
pages = "959-967",
doi = "10.1007/s12031-014-0452-y"
}

Drakulić, D. R., Stanojlović, M. R., Nedeljković, N., Grković, I., Velickovic, N., Guševac, I., Mitrović, N. Lj., Buzadzic, I.,& Horvat, A.. (2015). Upregulation of Nucleoside Triphosphate Diphosphohydrolase-1 and Ecto-5-Nucleotidase in Rat Hippocampus after Repeated Low-Dose Dexamethasone Administration. in Journal of Molecular Neuroscience, 55(4), 959-967.
https://doi.org/10.1007/s12031-014-0452-y

Drakulić DR, Stanojlović MR, Nedeljković N, Grković I, Velickovic N, Guševac I, Mitrović NL, Buzadzic I, Horvat A. Upregulation of Nucleoside Triphosphate Diphosphohydrolase-1 and Ecto-5-Nucleotidase in Rat Hippocampus after Repeated Low-Dose Dexamethasone Administration. in Journal of Molecular Neuroscience. 2015;55(4):959-967.
doi:10.1007/s12031-014-0452-y .

Drakulić, Dunja R., Stanojlović, Miloš R., Nedeljković, Nadežda, Grković, Ivana, Velickovic, Natasa, Guševac, Ivana, Mitrović, Nataša Lj., Buzadzic, Ivana, Horvat, Anica, "Upregulation of Nucleoside Triphosphate Diphosphohydrolase-1 and Ecto-5-Nucleotidase in Rat Hippocampus after Repeated Low-Dose Dexamethasone Administration" in Journal of Molecular Neuroscience, 55, no. 4 (2015):959-967,
https://doi.org/10.1007/s12031-014-0452-y . .

TY  - JOUR
AU  - Petrović, Snježana
AU  - Milošević, Maja
AU  - Ristic-Medic, Danijela
AU  - Velickovic, Natasa
AU  - Drakulić, Dunja R.
AU  - Grković, Ivana
AU  - Horvat, Anica
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/495
AB  - Our earlier studies found that in vitro estradiol modulates mitochondrial Ca2+ transport in discrete brain regions. The present study examined the role of estradiol receptors (ERs) in estradiol-induced inhibition of Ca2+ efflux from synaptosomal mitochondria isolated from rat caudate nuclei and brain stems. Radioactively labeled CaCl2 (0.6-0.75 mu Ci (CaCl2)-Ca-45) was used for Ca2+ transport monitoring. The results revealed that in the presence of ER antagonist 7 alpha, 17 beta-[9[(4,4,5,5,5-pentafluoropentyl) sulfinyl] nonyl] estra-1,3,5( 10)-triene-3,17-diol (ICI 182,780) (1 mu mol/L), the inhibitory effect of estradiol on mitochondrial Ca2+ efflux was more than 60% decreased, suggesting the involvement of ER in this mode of estradiol neuromodulatory action. When particular contributions of ER alpha and ER beta were tested, it was found that ER beta agonist 2,3-bis(4-hydroxy phenyl)-propionitrile (10 nmol/L) inhibited Ca2+ efflux more than 20%, while the inhibition with ER alpha agonist 4,4, 4-(4-propyl-[1H]-pyrazole-1,3,5-triyl) trisphenol (10 nmol/L) was about 10%, both compared to the control. Both agonists demonstrated attenuation of Ca2+ efflux decrease in the presence of mitochondrial Na+/Ca2+ exchanger antagonist 7-chloro-5-(2-chlorophenyl)-1,5-dihyhdro-4,1-benzothiazepin-2(3H)-one (10 mu mol/L), showing interference with the inhibitory action of that agent. Our results strongly indicate ERs as the mediators of estradiol-induced mitochondrial Ca2+ efflux inhibition in rat caudate nucleus and brain stem synaptosomes.
T2  - Turkish Journal of Biology
T1  - Estradiol receptors mediate estradiol-induced inhibition of mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem
VL  - 39
IS  - 2
SP  - 328
EP  - 334
DO  - 10.3906/biy-1408-62
ER  - 

@article{
author = "Petrović, Snježana and Milošević, Maja and Ristic-Medic, Danijela and Velickovic, Natasa and Drakulić, Dunja R. and Grković, Ivana and Horvat, Anica",
year = "2015",
abstract = "Our earlier studies found that in vitro estradiol modulates mitochondrial Ca2+ transport in discrete brain regions. The present study examined the role of estradiol receptors (ERs) in estradiol-induced inhibition of Ca2+ efflux from synaptosomal mitochondria isolated from rat caudate nuclei and brain stems. Radioactively labeled CaCl2 (0.6-0.75 mu Ci (CaCl2)-Ca-45) was used for Ca2+ transport monitoring. The results revealed that in the presence of ER antagonist 7 alpha, 17 beta-[9[(4,4,5,5,5-pentafluoropentyl) sulfinyl] nonyl] estra-1,3,5( 10)-triene-3,17-diol (ICI 182,780) (1 mu mol/L), the inhibitory effect of estradiol on mitochondrial Ca2+ efflux was more than 60% decreased, suggesting the involvement of ER in this mode of estradiol neuromodulatory action. When particular contributions of ER alpha and ER beta were tested, it was found that ER beta agonist 2,3-bis(4-hydroxy phenyl)-propionitrile (10 nmol/L) inhibited Ca2+ efflux more than 20%, while the inhibition with ER alpha agonist 4,4, 4-(4-propyl-[1H]-pyrazole-1,3,5-triyl) trisphenol (10 nmol/L) was about 10%, both compared to the control. Both agonists demonstrated attenuation of Ca2+ efflux decrease in the presence of mitochondrial Na+/Ca2+ exchanger antagonist 7-chloro-5-(2-chlorophenyl)-1,5-dihyhdro-4,1-benzothiazepin-2(3H)-one (10 mu mol/L), showing interference with the inhibitory action of that agent. Our results strongly indicate ERs as the mediators of estradiol-induced mitochondrial Ca2+ efflux inhibition in rat caudate nucleus and brain stem synaptosomes.",
journal = "Turkish Journal of Biology",
title = "Estradiol receptors mediate estradiol-induced inhibition of mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem",
volume = "39",
number = "2",
pages = "328-334",
doi = "10.3906/biy-1408-62"
}

Petrović, S., Milošević, M., Ristic-Medic, D., Velickovic, N., Drakulić, D. R., Grković, I.,& Horvat, A.. (2015). Estradiol receptors mediate estradiol-induced inhibition of mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem. in Turkish Journal of Biology, 39(2), 328-334.
https://doi.org/10.3906/biy-1408-62

Petrović S, Milošević M, Ristic-Medic D, Velickovic N, Drakulić DR, Grković I, Horvat A. Estradiol receptors mediate estradiol-induced inhibition of mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem. in Turkish Journal of Biology. 2015;39(2):328-334.
doi:10.3906/biy-1408-62 .

Petrović, Snježana, Milošević, Maja, Ristic-Medic, Danijela, Velickovic, Natasa, Drakulić, Dunja R., Grković, Ivana, Horvat, Anica, "Estradiol receptors mediate estradiol-induced inhibition of mitochondrial Ca2+ efflux in rat caudate nucleus and brain stem" in Turkish Journal of Biology, 39, no. 2 (2015):328-334,
https://doi.org/10.3906/biy-1408-62 . .

TY  - JOUR
AU  - Stanojlović, Miloš R.
AU  - Martinović, Jelena
AU  - Guševac, Ivana
AU  - Grković, Ivana
AU  - Mitrović, Nataša Lj.
AU  - Zarić, Marina
AU  - Horvat, Anica
AU  - Drakulić, Dunja R.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/527
AB  - Disturbance in blood circulation is associated with numerous pathological conditions characterized by cognitive decline and neurodegeneration. Activation of pro-apoptotic signaling previously detected in the synaptosomal fraction may underlie neurodegeneration in the prefrontal cortex of rats submitted to permanent bilateral common carotid arteries occlusion (two-vessel occlusion, 2VO). 17 beta-Estradiol (E) exerts potent neuroprotective effects in the brain affecting, among other, ischemia-induced pathological changes. As most significant changes in rats submitted to 2VO were observed on 7th day following the insult, of interest was to examine whether 7 day treatment with low dose of E (33.3 mu g/kg/day) prevents formerly reported neurodegeneration and may represent additional therapy during the early post-ischemic period. Role of E treatment on apoptotic pathway was monitored on Bcl-2 family members, cytochrome c, caspase 3 and PARP protein level in the synaptosomal (P2) fraction of the prefrontal cortex. Furthermore, changes of these proteins were examined in the cytosolic, mitochondrial and nuclear fraction, with the emphasis on potential involvement of extracellular signal-regulated kinases (ERK) and protein kinase B (Akt) activation and their role in nuclear translocation of transcriptional nuclear factor kappa B (NF-kB) associated with alteration of Box and Bcl-2 gene expression. The extent of cellular damage was determined using DNA fragmentation and Fluoro-Jade B staining. The absence of activation of apoptotic cascade both in the P2 and cell accompanied with decreased DNA fragmentation and number of degenerating neurons clearly indicates that E treatment ensures the efficient protection against ischemic insult. Moreover, E-mediated modulation of pro-apoptotic signaling in the cortical cellular fractions involves cooperative activation of ERK and Akt, which may be implicated in the observed prevention of neurodegenerative changes. (C) 2015 Elsevier Ltd. All rights reserved.
T2  - Neurochemistry International
T1  - Repeated low-dose 17 beta-estradiol treatment prevents activation of apoptotic signaling both in the synaptosomal and cellular fraction in rat prefrontal cortex following cerebral ischemia
VL  - 83-84
SP  - 1
EP  - 8
DO  - 10.1016/j.neuint.2015.03.002
ER  - 

@article{
author = "Stanojlović, Miloš R. and Martinović, Jelena and Guševac, Ivana and Grković, Ivana and Mitrović, Nataša Lj. and Zarić, Marina and Horvat, Anica and Drakulić, Dunja R.",
year = "2015",
abstract = "Disturbance in blood circulation is associated with numerous pathological conditions characterized by cognitive decline and neurodegeneration. Activation of pro-apoptotic signaling previously detected in the synaptosomal fraction may underlie neurodegeneration in the prefrontal cortex of rats submitted to permanent bilateral common carotid arteries occlusion (two-vessel occlusion, 2VO). 17 beta-Estradiol (E) exerts potent neuroprotective effects in the brain affecting, among other, ischemia-induced pathological changes. As most significant changes in rats submitted to 2VO were observed on 7th day following the insult, of interest was to examine whether 7 day treatment with low dose of E (33.3 mu g/kg/day) prevents formerly reported neurodegeneration and may represent additional therapy during the early post-ischemic period. Role of E treatment on apoptotic pathway was monitored on Bcl-2 family members, cytochrome c, caspase 3 and PARP protein level in the synaptosomal (P2) fraction of the prefrontal cortex. Furthermore, changes of these proteins were examined in the cytosolic, mitochondrial and nuclear fraction, with the emphasis on potential involvement of extracellular signal-regulated kinases (ERK) and protein kinase B (Akt) activation and their role in nuclear translocation of transcriptional nuclear factor kappa B (NF-kB) associated with alteration of Box and Bcl-2 gene expression. The extent of cellular damage was determined using DNA fragmentation and Fluoro-Jade B staining. The absence of activation of apoptotic cascade both in the P2 and cell accompanied with decreased DNA fragmentation and number of degenerating neurons clearly indicates that E treatment ensures the efficient protection against ischemic insult. Moreover, E-mediated modulation of pro-apoptotic signaling in the cortical cellular fractions involves cooperative activation of ERK and Akt, which may be implicated in the observed prevention of neurodegenerative changes. (C) 2015 Elsevier Ltd. All rights reserved.",
journal = "Neurochemistry International",
title = "Repeated low-dose 17 beta-estradiol treatment prevents activation of apoptotic signaling both in the synaptosomal and cellular fraction in rat prefrontal cortex following cerebral ischemia",
volume = "83-84",
pages = "1-8",
doi = "10.1016/j.neuint.2015.03.002"
}

Stanojlović, M. R., Martinović, J., Guševac, I., Grković, I., Mitrović, N. Lj., Zarić, M., Horvat, A.,& Drakulić, D. R.. (2015). Repeated low-dose 17 beta-estradiol treatment prevents activation of apoptotic signaling both in the synaptosomal and cellular fraction in rat prefrontal cortex following cerebral ischemia. in Neurochemistry International, 83-84, 1-8.
https://doi.org/10.1016/j.neuint.2015.03.002

Stanojlović MR, Martinović J, Guševac I, Grković I, Mitrović NL, Zarić M, Horvat A, Drakulić DR. Repeated low-dose 17 beta-estradiol treatment prevents activation of apoptotic signaling both in the synaptosomal and cellular fraction in rat prefrontal cortex following cerebral ischemia. in Neurochemistry International. 2015;83-84:1-8.
doi:10.1016/j.neuint.2015.03.002 .

Stanojlović, Miloš R., Martinović, Jelena, Guševac, Ivana, Grković, Ivana, Mitrović, Nataša Lj., Zarić, Marina, Horvat, Anica, Drakulić, Dunja R., "Repeated low-dose 17 beta-estradiol treatment prevents activation of apoptotic signaling both in the synaptosomal and cellular fraction in rat prefrontal cortex following cerebral ischemia" in Neurochemistry International, 83-84 (2015):1-8,
https://doi.org/10.1016/j.neuint.2015.03.002 . .

TY  - JOUR
AU  - Stanojlović, Miloš R.
AU  - Guševac, Ivana
AU  - Grković, Ivana
AU  - Martinović, Jelena
AU  - Mitrović, Nataša Lj.
AU  - Zarić, Marina
AU  - Horvat, Anica
AU  - Drakulić, Dunja R.
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/846
AB  - The present study attempted to investigate how chronic cerebral hypoperfusion (CCH) and repeated low-dose progesterone (P) treatment affect gene and protein expression, subcellular distribution of key apoptotic elements within protein kinase B (Akt) and extracellular signal-regulated kinases (Erk) signal transduction pathways, as well as neurodegenerative processes and behavior. The results revealed the absence of Erk activation in CCH in cytosolic and synaptosomal fractions, indicating a lower threshold of Akt activation in brain ischemia, while P increased their levels above control values. CCH induced an increase in caspase 3 (Casp 3) and poly (ADP-ribose) polymerase (PARP) gene and protein expression. However, P restored expression of examined molecules in all observed fractions, except for the levels of Casp 3 in synapses which highlighted its possible non-apoptotic or even protective function. Our study showed the absence of nuclear factor kappa-light-chain-enhancer of activated b cells (NF-kappa B) response to this type of ischemic condition and its strong activation under the influence of P. Further, the initial increase in the number of apoptotic cells and amount of DNA fragmentation induced by CCH was significantly reduced by P. Finally, P reversed the CCH-induced reduction in locomotor activity, while promoting a substantial decrease in anxiety-related behavior. Our findings support the concept that repeated low-dose post-ischemic P treatment reduces CCH-induced neurodegeneration in the hippocampus. Neuroprotection is initiated through the activation of investigated kinases and regulation of their downstream molecules in subcellular specific manner, indicating that this treatment may be a promising therapy for alleviation of CCH-induced pathologies. (C) 2015 IBRO. Published by Elsevier Ltd. All rights reserved.
PB  - Elsevier
T2  - Neuroscience
T1  - Effects of Chronic Cerebral Hypoperfusion and Low-Dose Progesterone Treatment on Apoptotic Processes, Expression and Subcellular Localization of Key Elements Within Akt and Erk Signaling Pathways in Rat Hippocampus
VL  - 311
SP  - 308
EP  - 321
DO  - 10.1016/j.neuroscience.2015.10.040
ER  - 

@article{
author = "Stanojlović, Miloš R. and Guševac, Ivana and Grković, Ivana and Martinović, Jelena and Mitrović, Nataša Lj. and Zarić, Marina and Horvat, Anica and Drakulić, Dunja R.",
year = "2015",
abstract = "The present study attempted to investigate how chronic cerebral hypoperfusion (CCH) and repeated low-dose progesterone (P) treatment affect gene and protein expression, subcellular distribution of key apoptotic elements within protein kinase B (Akt) and extracellular signal-regulated kinases (Erk) signal transduction pathways, as well as neurodegenerative processes and behavior. The results revealed the absence of Erk activation in CCH in cytosolic and synaptosomal fractions, indicating a lower threshold of Akt activation in brain ischemia, while P increased their levels above control values. CCH induced an increase in caspase 3 (Casp 3) and poly (ADP-ribose) polymerase (PARP) gene and protein expression. However, P restored expression of examined molecules in all observed fractions, except for the levels of Casp 3 in synapses which highlighted its possible non-apoptotic or even protective function. Our study showed the absence of nuclear factor kappa-light-chain-enhancer of activated b cells (NF-kappa B) response to this type of ischemic condition and its strong activation under the influence of P. Further, the initial increase in the number of apoptotic cells and amount of DNA fragmentation induced by CCH was significantly reduced by P. Finally, P reversed the CCH-induced reduction in locomotor activity, while promoting a substantial decrease in anxiety-related behavior. Our findings support the concept that repeated low-dose post-ischemic P treatment reduces CCH-induced neurodegeneration in the hippocampus. Neuroprotection is initiated through the activation of investigated kinases and regulation of their downstream molecules in subcellular specific manner, indicating that this treatment may be a promising therapy for alleviation of CCH-induced pathologies. (C) 2015 IBRO. Published by Elsevier Ltd. All rights reserved.",
publisher = "Elsevier",
journal = "Neuroscience",
title = "Effects of Chronic Cerebral Hypoperfusion and Low-Dose Progesterone Treatment on Apoptotic Processes, Expression and Subcellular Localization of Key Elements Within Akt and Erk Signaling Pathways in Rat Hippocampus",
volume = "311",
pages = "308-321",
doi = "10.1016/j.neuroscience.2015.10.040"
}

Stanojlović, M. R., Guševac, I., Grković, I., Martinović, J., Mitrović, N. Lj., Zarić, M., Horvat, A.,& Drakulić, D. R.. (2015). Effects of Chronic Cerebral Hypoperfusion and Low-Dose Progesterone Treatment on Apoptotic Processes, Expression and Subcellular Localization of Key Elements Within Akt and Erk Signaling Pathways in Rat Hippocampus. in Neuroscience
Elsevier., 311, 308-321.
https://doi.org/10.1016/j.neuroscience.2015.10.040

Stanojlović MR, Guševac I, Grković I, Martinović J, Mitrović NL, Zarić M, Horvat A, Drakulić DR. Effects of Chronic Cerebral Hypoperfusion and Low-Dose Progesterone Treatment on Apoptotic Processes, Expression and Subcellular Localization of Key Elements Within Akt and Erk Signaling Pathways in Rat Hippocampus. in Neuroscience. 2015;311:308-321.
doi:10.1016/j.neuroscience.2015.10.040 .

Stanojlović, Miloš R., Guševac, Ivana, Grković, Ivana, Martinović, Jelena, Mitrović, Nataša Lj., Zarić, Marina, Horvat, Anica, Drakulić, Dunja R., "Effects of Chronic Cerebral Hypoperfusion and Low-Dose Progesterone Treatment on Apoptotic Processes, Expression and Subcellular Localization of Key Elements Within Akt and Erk Signaling Pathways in Rat Hippocampus" in Neuroscience, 311 (2015):308-321,
https://doi.org/10.1016/j.neuroscience.2015.10.040 . .

TY  - JOUR
AU  - Stanojlović, Miloš R.
AU  - Horvat, Anica
AU  - Guševac, Ivana
AU  - Grković, Ivana
AU  - Mitrović, Nataša Lj.
AU  - Buzadzic, I.
AU  - Drakulić, Dunja R.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5445
AB  - To study time-dependent and gender-specific intracellular and biochemical mechanisms that lead to neurodegeneration due to moderate but persistent reduction of cerebral blood flow, adult male and female Wistar rats were divided into two main groups controls that underwent sham operation and animals subjected to permanent bilateral occlusion of common carotid arteries. Animals were sacrificed 3, 7 or 90 days following the insult. Expression of several apoptotic proteins in synaptic fractions along with Fluoro-Jade B staining and DNA fragmentation assay were used to estimate the apoptotic processes and potential neurodegeneration in cerebral cortex. Data suggest a time-specific increase of Bax as well as time- and gender-associated down-regulation in protein expression of Bcl-2, up-regulation of procaspase 3, accompanied with increased cleavage of procaspase 3 and PARP in synaptic terminals. Furthermore, time- but not gender-specific neurodegeneration was observed. Our findings support the concept of time- and gender-associated response to permanent bilateral occlusion of common carotid arteries, which would enable better understanding of the mechanisms underlying cerebral hypoperfusion.
T2  - Folia Biologica
T1  - Time Course of Cerebral Hypoperfusion-Induced Neurodegenerative Changes in the Cortex of Male and Female Rats
VL  - 60
IS  - 3
SP  - 123
EP  - 132
UR  - https://hdl.handle.net/21.15107/rcub_vinar_5445
ER  - 

@article{
author = "Stanojlović, Miloš R. and Horvat, Anica and Guševac, Ivana and Grković, Ivana and Mitrović, Nataša Lj. and Buzadzic, I. and Drakulić, Dunja R.",
year = "2014",
abstract = "To study time-dependent and gender-specific intracellular and biochemical mechanisms that lead to neurodegeneration due to moderate but persistent reduction of cerebral blood flow, adult male and female Wistar rats were divided into two main groups controls that underwent sham operation and animals subjected to permanent bilateral occlusion of common carotid arteries. Animals were sacrificed 3, 7 or 90 days following the insult. Expression of several apoptotic proteins in synaptic fractions along with Fluoro-Jade B staining and DNA fragmentation assay were used to estimate the apoptotic processes and potential neurodegeneration in cerebral cortex. Data suggest a time-specific increase of Bax as well as time- and gender-associated down-regulation in protein expression of Bcl-2, up-regulation of procaspase 3, accompanied with increased cleavage of procaspase 3 and PARP in synaptic terminals. Furthermore, time- but not gender-specific neurodegeneration was observed. Our findings support the concept of time- and gender-associated response to permanent bilateral occlusion of common carotid arteries, which would enable better understanding of the mechanisms underlying cerebral hypoperfusion.",
journal = "Folia Biologica",
title = "Time Course of Cerebral Hypoperfusion-Induced Neurodegenerative Changes in the Cortex of Male and Female Rats",
volume = "60",
number = "3",
pages = "123-132",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5445"
}

Stanojlović, M. R., Horvat, A., Guševac, I., Grković, I., Mitrović, N. Lj., Buzadzic, I.,& Drakulić, D. R.. (2014). Time Course of Cerebral Hypoperfusion-Induced Neurodegenerative Changes in the Cortex of Male and Female Rats. in Folia Biologica, 60(3), 123-132.
https://hdl.handle.net/21.15107/rcub_vinar_5445

Stanojlović MR, Horvat A, Guševac I, Grković I, Mitrović NL, Buzadzic I, Drakulić DR. Time Course of Cerebral Hypoperfusion-Induced Neurodegenerative Changes in the Cortex of Male and Female Rats. in Folia Biologica. 2014;60(3):123-132.
https://hdl.handle.net/21.15107/rcub_vinar_5445 .

Stanojlović, Miloš R., Horvat, Anica, Guševac, Ivana, Grković, Ivana, Mitrović, Nataša Lj., Buzadzic, I., Drakulić, Dunja R., "Time Course of Cerebral Hypoperfusion-Induced Neurodegenerative Changes in the Cortex of Male and Female Rats" in Folia Biologica, 60, no. 3 (2014):123-132,
https://hdl.handle.net/21.15107/rcub_vinar_5445 .

TY  - JOUR
AU  - Grković, Ivana
AU  - Bjelobaba, Ivana
AU  - Nedeljković, Nadežda
AU  - Mitrović, Nataša Lj.
AU  - Drakulić, Dunja R.
AU  - Stanojlović, Miloš R.
AU  - Horvat, Anica
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/64
AB  - Ecto-5-nucleotidase (e-5NT), a glycosylphosphatidylinositol-linked membrane protein, catalyzes a conversion of AMP to adenosine, which influences nearly every aspect of brain physiology, including embryonic and postnatal brain development. The present study aimed to investigate a pattern of expression, activity and kinetic properties of e-5NT in the hippocampal formation and synaptic plasma membrane (SPM) preparations in rats at postnatal days (PDs) 7, 15, 20, 30 and 90. By combining gene expression analysis and enzyme histochemistry, we observed that e-5NT mRNA reached the adult level at PD20, while the enzyme activity continued to increase beyond this age. Further analysis revealed that hippocampal layers rich in synapses expressed the highest levels of e-5NT activity, while in layers populated with neuronal cell bodies, the enzyme activity was weak or absent. Therefore, activity and expression of e-5NT were analyzed in SPM preparations isolated from rats at different ages. The presence of two protein bands of about 65 and 68 kDa was determined by immunoblot analysis. The 65-kDa band was present at all ages, and its abundance increased from PD7 to PD20. The 68-kDa band appeared at PD15 and increased until PD30, coinciding with the increase of e-5NT activity, substrate affinity and enzymatic efficiency. Since distinct e-5NT isoforms may derive from different patterns of the enzyme protein N-glycosylation, we speculate that long-term regulation of e-5NT activity in adulthood may be effectuated at posttranslational level and without overall change in the gene and protein expression.
T2  - Journal of Molecular Neuroscience
T1  - Developmental Increase in Ecto-5-Nucleotidase Activity Overlaps with Appearance of Two Immunologically Distinct Enzyme Isoforms in Rat Hippocampal Synaptic Plasma Membranes
VL  - 54
IS  - 1
SP  - 109
EP  - 118
DO  - 10.1007/s12031-014-0256-0
ER  - 

@article{
author = "Grković, Ivana and Bjelobaba, Ivana and Nedeljković, Nadežda and Mitrović, Nataša Lj. and Drakulić, Dunja R. and Stanojlović, Miloš R. and Horvat, Anica",
year = "2014",
abstract = "Ecto-5-nucleotidase (e-5NT), a glycosylphosphatidylinositol-linked membrane protein, catalyzes a conversion of AMP to adenosine, which influences nearly every aspect of brain physiology, including embryonic and postnatal brain development. The present study aimed to investigate a pattern of expression, activity and kinetic properties of e-5NT in the hippocampal formation and synaptic plasma membrane (SPM) preparations in rats at postnatal days (PDs) 7, 15, 20, 30 and 90. By combining gene expression analysis and enzyme histochemistry, we observed that e-5NT mRNA reached the adult level at PD20, while the enzyme activity continued to increase beyond this age. Further analysis revealed that hippocampal layers rich in synapses expressed the highest levels of e-5NT activity, while in layers populated with neuronal cell bodies, the enzyme activity was weak or absent. Therefore, activity and expression of e-5NT were analyzed in SPM preparations isolated from rats at different ages. The presence of two protein bands of about 65 and 68 kDa was determined by immunoblot analysis. The 65-kDa band was present at all ages, and its abundance increased from PD7 to PD20. The 68-kDa band appeared at PD15 and increased until PD30, coinciding with the increase of e-5NT activity, substrate affinity and enzymatic efficiency. Since distinct e-5NT isoforms may derive from different patterns of the enzyme protein N-glycosylation, we speculate that long-term regulation of e-5NT activity in adulthood may be effectuated at posttranslational level and without overall change in the gene and protein expression.",
journal = "Journal of Molecular Neuroscience",
title = "Developmental Increase in Ecto-5-Nucleotidase Activity Overlaps with Appearance of Two Immunologically Distinct Enzyme Isoforms in Rat Hippocampal Synaptic Plasma Membranes",
volume = "54",
number = "1",
pages = "109-118",
doi = "10.1007/s12031-014-0256-0"
}

Grković, I., Bjelobaba, I., Nedeljković, N., Mitrović, N. Lj., Drakulić, D. R., Stanojlović, M. R.,& Horvat, A.. (2014). Developmental Increase in Ecto-5-Nucleotidase Activity Overlaps with Appearance of Two Immunologically Distinct Enzyme Isoforms in Rat Hippocampal Synaptic Plasma Membranes. in Journal of Molecular Neuroscience, 54(1), 109-118.
https://doi.org/10.1007/s12031-014-0256-0

Grković I, Bjelobaba I, Nedeljković N, Mitrović NL, Drakulić DR, Stanojlović MR, Horvat A. Developmental Increase in Ecto-5-Nucleotidase Activity Overlaps with Appearance of Two Immunologically Distinct Enzyme Isoforms in Rat Hippocampal Synaptic Plasma Membranes. in Journal of Molecular Neuroscience. 2014;54(1):109-118.
doi:10.1007/s12031-014-0256-0 .

Grković, Ivana, Bjelobaba, Ivana, Nedeljković, Nadežda, Mitrović, Nataša Lj., Drakulić, Dunja R., Stanojlović, Miloš R., Horvat, Anica, "Developmental Increase in Ecto-5-Nucleotidase Activity Overlaps with Appearance of Two Immunologically Distinct Enzyme Isoforms in Rat Hippocampal Synaptic Plasma Membranes" in Journal of Molecular Neuroscience, 54, no. 1 (2014):109-118,
https://doi.org/10.1007/s12031-014-0256-0 . .

TY  - JOUR
AU  - Stanojlović, Miloš R.
AU  - Guševac, Ivana
AU  - Grković, Ivana
AU  - Mitrović, Nataša Lj.
AU  - Horvat, Anica
AU  - Drakulić, Dunja R.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/301
AB  - Although the model of cerebral hypoperfusion in rats has been a matter of many investigations over the years, the exact intracellular and biochemical mechanisms that lead to neuron loss and memory decline have not been clearly identified. In the current study, we examined whether cerebral hypoperfusion causes changes in hippocampal protein expression of apoptotic markers in the synaptosomal fraction and neurodegeneration in a time-dependent and sex-specific manner. Adult male and female Wistar rats were divided into two main groups, controls that underwent sham operation, and animals subjected to permanent bilateral occlusion of common carotid arteries. Both male and female rats were killed 3, 7 or 90 days following the insult. The obtained results indicate that the peak of processes that lead to apoptosis occured on postoperative day 7 and that they were more prominent in males, indicating that neuroprotective effects of certain substances (planned for future experiments), should be tested at this time point.
T2  - Archives of Biological Sciences
T1  - Time-Related Sex Diffrences in Cerebral Hypoperfusion-Induced Brain Injury
VL  - 66
IS  - 4
SP  - 1673
EP  - 1680
DO  - 10.2298/ABS1404673S
ER  - 

@article{
author = "Stanojlović, Miloš R. and Guševac, Ivana and Grković, Ivana and Mitrović, Nataša Lj. and Horvat, Anica and Drakulić, Dunja R.",
year = "2014",
abstract = "Although the model of cerebral hypoperfusion in rats has been a matter of many investigations over the years, the exact intracellular and biochemical mechanisms that lead to neuron loss and memory decline have not been clearly identified. In the current study, we examined whether cerebral hypoperfusion causes changes in hippocampal protein expression of apoptotic markers in the synaptosomal fraction and neurodegeneration in a time-dependent and sex-specific manner. Adult male and female Wistar rats were divided into two main groups, controls that underwent sham operation, and animals subjected to permanent bilateral occlusion of common carotid arteries. Both male and female rats were killed 3, 7 or 90 days following the insult. The obtained results indicate that the peak of processes that lead to apoptosis occured on postoperative day 7 and that they were more prominent in males, indicating that neuroprotective effects of certain substances (planned for future experiments), should be tested at this time point.",
journal = "Archives of Biological Sciences",
title = "Time-Related Sex Diffrences in Cerebral Hypoperfusion-Induced Brain Injury",
volume = "66",
number = "4",
pages = "1673-1680",
doi = "10.2298/ABS1404673S"
}

Stanojlović, M. R., Guševac, I., Grković, I., Mitrović, N. Lj., Horvat, A.,& Drakulić, D. R.. (2014). Time-Related Sex Diffrences in Cerebral Hypoperfusion-Induced Brain Injury. in Archives of Biological Sciences, 66(4), 1673-1680.
https://doi.org/10.2298/ABS1404673S

Stanojlović MR, Guševac I, Grković I, Mitrović NL, Horvat A, Drakulić DR. Time-Related Sex Diffrences in Cerebral Hypoperfusion-Induced Brain Injury. in Archives of Biological Sciences. 2014;66(4):1673-1680.
doi:10.2298/ABS1404673S .

Stanojlović, Miloš R., Guševac, Ivana, Grković, Ivana, Mitrović, Nataša Lj., Horvat, Anica, Drakulić, Dunja R., "Time-Related Sex Diffrences in Cerebral Hypoperfusion-Induced Brain Injury" in Archives of Biological Sciences, 66, no. 4 (2014):1673-1680,
https://doi.org/10.2298/ABS1404673S . .

TY  - JOUR
AU  - Velickovic, Natasa A.
AU  - Đorđević, Ana D.
AU  - Drakulić, Dunja R.
AU  - Šećerov, Bojana Lj.
AU  - Grković, Ivana
AU  - Milošević, Maja
AU  - Horvat, Anica
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5594
AB  - Ionizing radiation is commonly used in the treatment of brain tumors but it can impair cognitive functions, such as learning and memory. Since cognitive dysfunctions are predominantly result of cell death by apoptosis in hippocampal cells, in this study we analyzed acute effects of cranial gamma-irradiation (10 Gy) on-expression-of proapoptotic molecules (p53, Box) and antiapoptotic molecule Bcl-2, as well as caspase-3 activation and cytochrome c redistribution in the hippocampus of young rats. The selected regimen of irradiation resembles the established animal model for childhood prophylactic cranial radiotherapy. Our results demonstrated that p53 mRNA expression was unchanged after irradiation, while induction of p53 protein was rapid. In parallel, Bax mRNA and protein levels were also increased following irradiation, whereas Bcl-2 expression was not changed during the examined post-irradiation period. These changes were accompanied with early hallmarks of apoptosis, such as increased cytochrome c release and stimulated activation of caspase-3. Overall, this study demonstrates that cranial irradiation is associated with the augmented apoptotic pathway in the rat hippocampus, which could be related to the cognitive decline observed in patients after prophylactic cranial radiotherapy, but also opens perspective in finding radioprotectors that can mitigate radiation injury of normal brain tissue.
T2  - Nuclear technology and radiation protection
T1  - Radiation-Mediated Induction of Apoptotic Cell Death in Rat Hippocampus
VL  - 28
IS  - 2
SP  - 212
EP  - 220
DO  - 10.2298/NTRP1302212V
ER  - 

@article{
author = "Velickovic, Natasa A. and Đorđević, Ana D. and Drakulić, Dunja R. and Šećerov, Bojana Lj. and Grković, Ivana and Milošević, Maja and Horvat, Anica",
year = "2013",
abstract = "Ionizing radiation is commonly used in the treatment of brain tumors but it can impair cognitive functions, such as learning and memory. Since cognitive dysfunctions are predominantly result of cell death by apoptosis in hippocampal cells, in this study we analyzed acute effects of cranial gamma-irradiation (10 Gy) on-expression-of proapoptotic molecules (p53, Box) and antiapoptotic molecule Bcl-2, as well as caspase-3 activation and cytochrome c redistribution in the hippocampus of young rats. The selected regimen of irradiation resembles the established animal model for childhood prophylactic cranial radiotherapy. Our results demonstrated that p53 mRNA expression was unchanged after irradiation, while induction of p53 protein was rapid. In parallel, Bax mRNA and protein levels were also increased following irradiation, whereas Bcl-2 expression was not changed during the examined post-irradiation period. These changes were accompanied with early hallmarks of apoptosis, such as increased cytochrome c release and stimulated activation of caspase-3. Overall, this study demonstrates that cranial irradiation is associated with the augmented apoptotic pathway in the rat hippocampus, which could be related to the cognitive decline observed in patients after prophylactic cranial radiotherapy, but also opens perspective in finding radioprotectors that can mitigate radiation injury of normal brain tissue.",
journal = "Nuclear technology and radiation protection",
title = "Radiation-Mediated Induction of Apoptotic Cell Death in Rat Hippocampus",
volume = "28",
number = "2",
pages = "212-220",
doi = "10.2298/NTRP1302212V"
}

Velickovic, N. A., Đorđević, A. D., Drakulić, D. R., Šećerov, B. Lj., Grković, I., Milošević, M.,& Horvat, A.. (2013). Radiation-Mediated Induction of Apoptotic Cell Death in Rat Hippocampus. in Nuclear technology and radiation protection, 28(2), 212-220.
https://doi.org/10.2298/NTRP1302212V

Velickovic NA, Đorđević AD, Drakulić DR, Šećerov BL, Grković I, Milošević M, Horvat A. Radiation-Mediated Induction of Apoptotic Cell Death in Rat Hippocampus. in Nuclear technology and radiation protection. 2013;28(2):212-220.
doi:10.2298/NTRP1302212V .

Velickovic, Natasa A., Đorđević, Ana D., Drakulić, Dunja R., Šećerov, Bojana Lj., Grković, Ivana, Milošević, Maja, Horvat, Anica, "Radiation-Mediated Induction of Apoptotic Cell Death in Rat Hippocampus" in Nuclear technology and radiation protection, 28, no. 2 (2013):212-220,
https://doi.org/10.2298/NTRP1302212V . .

TY  - JOUR
AU  - Drakulić, Dunja R.
AU  - Velickovic, N.
AU  - Stanojlović, Miloš R.
AU  - Grković, Ivana
AU  - Mitrović, Nataša Lj.
AU  - Lavrnja, I.
AU  - Horvat, Anica
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5549
AB  - Synthetic glucocorticoid dexamethasone (DEX), a highly potent anti-inflammatory and immunosuppressive agent, is widely used in the treatment of brain cancer, as well as for inflammatory and autoimmune diseases. The present study aimed to determine whether low-dose subchronic DEX treatment (100g/kg for eight consecutive days) exerts long-term effects on apoptosis in the adult rat prefrontal cortex (PFC) by examining the expression of cell death-promoting molecules [poly(ADP-ribose) polymerase (PARP), p53, procaspase 3, cleaved caspase 3, Bax] and cell-survival molecules (AKT, Bcl-2). The results obtained revealed that body, thymus and adrenal gland weights, as well corticosterone levels, in the serum and PFC were reduced 1day after the last DEX injection. In the PFC, DEX caused activation of AKT, augmentation of pro-survival Bcl-2 protein and an enhanced Bcl-2/Bax protein ratio, as well Bcl-2 translocation to the mitochondria. An unaltered profile with respect to the protein expression of apoptotic molecules PARP, procaspase 3 and Bax was detected, whereas p53 protein was decreased. Reverse transcriptase -polymerase chain reaction analysis showed a decrease of p53 mRNA levels and no significant difference in Bcl-2 and Bax mRNA expression in DEX-treated rats. Finally, a DNA fragmentation assay and Fluoro-Jade staining demonstrated no considerable changes in apoptosis in the rat PFC. Our findings support the concept that low-dose DEX creates a hypocorticoid state in the brain and also indicate that subchronic DEX treatment activates the pro-survival signalling pathway but does not change apoptotic markers in the rat PFC. This mechanism might be relevant for the DEX-induced apoptosis resistance observed during and after chemotherapy of patients with brain tumours.
T2  - Journal of Neuroendocrinology
T1  - Low-Dose Dexamethasone Treatment Promotes the Pro-Survival Signalling Pathway in the Adult Rat Prefrontal Cortex
VL  - 25
IS  - 7
SP  - 605
EP  - 616
DO  - 10.1111/jne.12037
ER  - 

@article{
author = "Drakulić, Dunja R. and Velickovic, N. and Stanojlović, Miloš R. and Grković, Ivana and Mitrović, Nataša Lj. and Lavrnja, I. and Horvat, Anica",
year = "2013",
abstract = "Synthetic glucocorticoid dexamethasone (DEX), a highly potent anti-inflammatory and immunosuppressive agent, is widely used in the treatment of brain cancer, as well as for inflammatory and autoimmune diseases. The present study aimed to determine whether low-dose subchronic DEX treatment (100g/kg for eight consecutive days) exerts long-term effects on apoptosis in the adult rat prefrontal cortex (PFC) by examining the expression of cell death-promoting molecules [poly(ADP-ribose) polymerase (PARP), p53, procaspase 3, cleaved caspase 3, Bax] and cell-survival molecules (AKT, Bcl-2). The results obtained revealed that body, thymus and adrenal gland weights, as well corticosterone levels, in the serum and PFC were reduced 1day after the last DEX injection. In the PFC, DEX caused activation of AKT, augmentation of pro-survival Bcl-2 protein and an enhanced Bcl-2/Bax protein ratio, as well Bcl-2 translocation to the mitochondria. An unaltered profile with respect to the protein expression of apoptotic molecules PARP, procaspase 3 and Bax was detected, whereas p53 protein was decreased. Reverse transcriptase -polymerase chain reaction analysis showed a decrease of p53 mRNA levels and no significant difference in Bcl-2 and Bax mRNA expression in DEX-treated rats. Finally, a DNA fragmentation assay and Fluoro-Jade staining demonstrated no considerable changes in apoptosis in the rat PFC. Our findings support the concept that low-dose DEX creates a hypocorticoid state in the brain and also indicate that subchronic DEX treatment activates the pro-survival signalling pathway but does not change apoptotic markers in the rat PFC. This mechanism might be relevant for the DEX-induced apoptosis resistance observed during and after chemotherapy of patients with brain tumours.",
journal = "Journal of Neuroendocrinology",
title = "Low-Dose Dexamethasone Treatment Promotes the Pro-Survival Signalling Pathway in the Adult Rat Prefrontal Cortex",
volume = "25",
number = "7",
pages = "605-616",
doi = "10.1111/jne.12037"
}

Drakulić, D. R., Velickovic, N., Stanojlović, M. R., Grković, I., Mitrović, N. Lj., Lavrnja, I.,& Horvat, A.. (2013). Low-Dose Dexamethasone Treatment Promotes the Pro-Survival Signalling Pathway in the Adult Rat Prefrontal Cortex. in Journal of Neuroendocrinology, 25(7), 605-616.
https://doi.org/10.1111/jne.12037

Drakulić DR, Velickovic N, Stanojlović MR, Grković I, Mitrović NL, Lavrnja I, Horvat A. Low-Dose Dexamethasone Treatment Promotes the Pro-Survival Signalling Pathway in the Adult Rat Prefrontal Cortex. in Journal of Neuroendocrinology. 2013;25(7):605-616.
doi:10.1111/jne.12037 .

Drakulić, Dunja R., Velickovic, N., Stanojlović, Miloš R., Grković, Ivana, Mitrović, Nataša Lj., Lavrnja, I., Horvat, Anica, "Low-Dose Dexamethasone Treatment Promotes the Pro-Survival Signalling Pathway in the Adult Rat Prefrontal Cortex" in Journal of Neuroendocrinology, 25, no. 7 (2013):605-616,
https://doi.org/10.1111/jne.12037 . .

TY  - JOUR
AU  - Petrović, Snježana
AU  - Milošević, Maja
AU  - Drakulić, Dunja R.
AU  - Grković, Ivana
AU  - Stanojlović, Miloš R.
AU  - Mitrović, Nataša Lj.
AU  - Horvat, Anica
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5012
AB  - The aim of this study was to examine the rapid non-genomic effect of 17 beta-estradiol (E2) on Ca2+ transport in mitochondria isolated from the nerve terminals (synaptosomes) of caudate nuclei (NC) and brain stems (BS) of ovariectomised female rats. In physiological conditions no effect of E2 on Ca2+ influx into synaptosomal mitochondria through ruthenium red (RR)-sensitive uniporter was observed. However, in the presence of uncoupling agent carbonyl cyanide4-(trifluoromethoxy)phenylhydrazone (FCCP) (1 mu mol/l), pre-treatment with 0.5 nmol/l E2 protected mitochondrial membrane potential and consequently increased Ca2+ influx (2.3-fold in NC and 3.1-fold in BS). At the same time, 0.5 nmol/l E2 by increasing the affinity of mitochondrial Na+/Ca2+ exchanger for Na+ inhibited mitochondrial Ca2+ efflux in NC and BS by about 40%. Also, the specific binding of physiological E2 concentrations (0.1-10 nmol/l) to isolated synaptosomal mitochondria was detected. Using membrane impermeable E2 bound to bovine serum albumin and selective inhibitor of mitochondrial Na+/Ca2+ exchanger, we obtained that E2s action on mitochondrial Ca2+ efflux at least partially is due to the direct effects on the mitochondrial membrane and/or Na+/Ca2+ exchanger located in inner mitochondrial membrane. Our results implicate E2 as a modulator of Ca2+ concentration in mitochondrial matrix, and ultimately in the cytosol. Given the vital role of Ca2+ in regulation of total nerve cells activity, especially energy metabolism, neurotransmission and directing the cells toward survival or cell death, the effects on mitochondrial Ca2+ transport could be one of the important modes of E2 neuromodulatory action independent of the genome. (C) 2012 IBRO. Published by Elsevier Ltd. All rights reserved.
T2  - Neuroscience
T1  - 17 beta-estradiol modulates mitochondrial Ca2+ flux in rat caudate nucleus and brain stem
VL  - 220
SP  - 32
EP  - 40
DO  - 10.1016/j.neuroscience.2012.06.040
ER  - 

@article{
author = "Petrović, Snježana and Milošević, Maja and Drakulić, Dunja R. and Grković, Ivana and Stanojlović, Miloš R. and Mitrović, Nataša Lj. and Horvat, Anica",
year = "2012",
abstract = "The aim of this study was to examine the rapid non-genomic effect of 17 beta-estradiol (E2) on Ca2+ transport in mitochondria isolated from the nerve terminals (synaptosomes) of caudate nuclei (NC) and brain stems (BS) of ovariectomised female rats. In physiological conditions no effect of E2 on Ca2+ influx into synaptosomal mitochondria through ruthenium red (RR)-sensitive uniporter was observed. However, in the presence of uncoupling agent carbonyl cyanide4-(trifluoromethoxy)phenylhydrazone (FCCP) (1 mu mol/l), pre-treatment with 0.5 nmol/l E2 protected mitochondrial membrane potential and consequently increased Ca2+ influx (2.3-fold in NC and 3.1-fold in BS). At the same time, 0.5 nmol/l E2 by increasing the affinity of mitochondrial Na+/Ca2+ exchanger for Na+ inhibited mitochondrial Ca2+ efflux in NC and BS by about 40%. Also, the specific binding of physiological E2 concentrations (0.1-10 nmol/l) to isolated synaptosomal mitochondria was detected. Using membrane impermeable E2 bound to bovine serum albumin and selective inhibitor of mitochondrial Na+/Ca2+ exchanger, we obtained that E2s action on mitochondrial Ca2+ efflux at least partially is due to the direct effects on the mitochondrial membrane and/or Na+/Ca2+ exchanger located in inner mitochondrial membrane. Our results implicate E2 as a modulator of Ca2+ concentration in mitochondrial matrix, and ultimately in the cytosol. Given the vital role of Ca2+ in regulation of total nerve cells activity, especially energy metabolism, neurotransmission and directing the cells toward survival or cell death, the effects on mitochondrial Ca2+ transport could be one of the important modes of E2 neuromodulatory action independent of the genome. (C) 2012 IBRO. Published by Elsevier Ltd. All rights reserved.",
journal = "Neuroscience",
title = "17 beta-estradiol modulates mitochondrial Ca2+ flux in rat caudate nucleus and brain stem",
volume = "220",
pages = "32-40",
doi = "10.1016/j.neuroscience.2012.06.040"
}

Petrović, S., Milošević, M., Drakulić, D. R., Grković, I., Stanojlović, M. R., Mitrović, N. Lj.,& Horvat, A.. (2012). 17 beta-estradiol modulates mitochondrial Ca2+ flux in rat caudate nucleus and brain stem. in Neuroscience, 220, 32-40.
https://doi.org/10.1016/j.neuroscience.2012.06.040

Petrović S, Milošević M, Drakulić DR, Grković I, Stanojlović MR, Mitrović NL, Horvat A. 17 beta-estradiol modulates mitochondrial Ca2+ flux in rat caudate nucleus and brain stem. in Neuroscience. 2012;220:32-40.
doi:10.1016/j.neuroscience.2012.06.040 .

Petrović, Snježana, Milošević, Maja, Drakulić, Dunja R., Grković, Ivana, Stanojlović, Miloš R., Mitrović, Nataša Lj., Horvat, Anica, "17 beta-estradiol modulates mitochondrial Ca2+ flux in rat caudate nucleus and brain stem" in Neuroscience, 220 (2012):32-40,
https://doi.org/10.1016/j.neuroscience.2012.06.040 . .

TY  - JOUR
AU  - Velickovic, Natasa
AU  - Drakulić, Dunja R.
AU  - Petrovic, Snjezana
AU  - Grković, Ivana
AU  - Milošević, Maja
AU  - Stanojlović, Miloš R.
AU  - Horvat, Anica
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5051
AB  - Recent studies reported that exposure of juvenile rats to cranial irradiation affects hypothalamic-pituitary-adrenal (HPA) axis stability, leading to its activation along with radiation-induced inflammation. In the present study, we hypothesized whether inflammatory reaction in the CNS could be a mediator of HPA axis response to cranial irradiation (CI). Therefore, we analyzed time-course changes of serum corticosterone level, as well IL-1 beta and TNF-alpha level in the serum and hypothalamus of juvenile rats after CI. Protein and gene expression of the glucocorticoid receptor (GR) and nuclear factor kappaB (NF kappa B) were examined in the hippocampus within 24 h postirradiation interval. Cranial irradiation led to rapid induction of both GR and NF kappa B mRNA and protein in the hippocampus at 1 h. The increment in NF kappa B protein persisted for 2 h, therefore NF kappa B/GR protein ratio was turned in favor of NF kappa B. Central inflammation was characterized by increased IL-1 beta in the hypothalamus, with maximum levels at 2 and 4 h after irradiation, while both IL-1 beta and TNF-alpha were undetectable in the serum. Enhanced hypothalamic IL-1 beta probably induced the relocation of hippocampal NF kappa B to the nucleus and decreased NF kappa B mRNA at 6 h, indicating promotion of inflammation in the key tissue for HPA axis regulation. Concomitant increase of corticosterone level and enhanced GR nuclear translocation in the hippocampus at 6 h might represent a compensatory mechanism for observed inflammation. Our results indicate that acute radiation response is characterized by increased central inflammation and concomitant HPA axis activation, most likely having a role in protection of the organism from overwhelming inflammatory reaction.
T2  - Cellular and Molecular Neurobiology
T1  - Time-Course of Hypothalamic-Pituitary-Adrenal Axis Activity and Inflammation in Juvenile Rat Brain After Cranial Irradiation
VL  - 32
IS  - 7
SP  - 1175
EP  - 1185
DO  - 10.1007/s10571-012-9843-1
ER  - 

@article{
author = "Velickovic, Natasa and Drakulić, Dunja R. and Petrovic, Snjezana and Grković, Ivana and Milošević, Maja and Stanojlović, Miloš R. and Horvat, Anica",
year = "2012",
abstract = "Recent studies reported that exposure of juvenile rats to cranial irradiation affects hypothalamic-pituitary-adrenal (HPA) axis stability, leading to its activation along with radiation-induced inflammation. In the present study, we hypothesized whether inflammatory reaction in the CNS could be a mediator of HPA axis response to cranial irradiation (CI). Therefore, we analyzed time-course changes of serum corticosterone level, as well IL-1 beta and TNF-alpha level in the serum and hypothalamus of juvenile rats after CI. Protein and gene expression of the glucocorticoid receptor (GR) and nuclear factor kappaB (NF kappa B) were examined in the hippocampus within 24 h postirradiation interval. Cranial irradiation led to rapid induction of both GR and NF kappa B mRNA and protein in the hippocampus at 1 h. The increment in NF kappa B protein persisted for 2 h, therefore NF kappa B/GR protein ratio was turned in favor of NF kappa B. Central inflammation was characterized by increased IL-1 beta in the hypothalamus, with maximum levels at 2 and 4 h after irradiation, while both IL-1 beta and TNF-alpha were undetectable in the serum. Enhanced hypothalamic IL-1 beta probably induced the relocation of hippocampal NF kappa B to the nucleus and decreased NF kappa B mRNA at 6 h, indicating promotion of inflammation in the key tissue for HPA axis regulation. Concomitant increase of corticosterone level and enhanced GR nuclear translocation in the hippocampus at 6 h might represent a compensatory mechanism for observed inflammation. Our results indicate that acute radiation response is characterized by increased central inflammation and concomitant HPA axis activation, most likely having a role in protection of the organism from overwhelming inflammatory reaction.",
journal = "Cellular and Molecular Neurobiology",
title = "Time-Course of Hypothalamic-Pituitary-Adrenal Axis Activity and Inflammation in Juvenile Rat Brain After Cranial Irradiation",
volume = "32",
number = "7",
pages = "1175-1185",
doi = "10.1007/s10571-012-9843-1"
}

Velickovic, N., Drakulić, D. R., Petrovic, S., Grković, I., Milošević, M., Stanojlović, M. R.,& Horvat, A.. (2012). Time-Course of Hypothalamic-Pituitary-Adrenal Axis Activity and Inflammation in Juvenile Rat Brain After Cranial Irradiation. in Cellular and Molecular Neurobiology, 32(7), 1175-1185.
https://doi.org/10.1007/s10571-012-9843-1

Velickovic N, Drakulić DR, Petrovic S, Grković I, Milošević M, Stanojlović MR, Horvat A. Time-Course of Hypothalamic-Pituitary-Adrenal Axis Activity and Inflammation in Juvenile Rat Brain After Cranial Irradiation. in Cellular and Molecular Neurobiology. 2012;32(7):1175-1185.
doi:10.1007/s10571-012-9843-1 .

Velickovic, Natasa, Drakulić, Dunja R., Petrovic, Snjezana, Grković, Ivana, Milošević, Maja, Stanojlović, Miloš R., Horvat, Anica, "Time-Course of Hypothalamic-Pituitary-Adrenal Axis Activity and Inflammation in Juvenile Rat Brain After Cranial Irradiation" in Cellular and Molecular Neurobiology, 32, no. 7 (2012):1175-1185,
https://doi.org/10.1007/s10571-012-9843-1 . .

TY  - JOUR
AU  - Milošević, Maja
AU  - Petrovic, Snjezana
AU  - Velickovic, Natasa
AU  - Grković, Ivana
AU  - Ignjatović, Marija
AU  - Horvat, Anica
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5131
AB  - Extracellular nucleotides affect female reproductive functions, fertilization, and pregnancy. The aim of this study was to investigate biochemical characteristics of ATP and ADP hydrolysis and identify E-NTPDases in myometrial cell membranes from Wistar albino rats. The apparent K (m) values were 506.4 +/- A 62.1 and 638.8 +/- A 31.3 mu M, with a calculated V (max) (app) of 3,973.0 +/- A 279.5 and 2,853.9 +/- A 79.8 nmol/min/mg for ATP and ADP, respectively. The enzyme activity described here has common properties characteristic for NTPDases: divalent cation dependence; alkaline pH optimum for both substrates, insensitivity to some of classical ATPase inhibitors (ouabain, oligomycine, theophylline, levamisole) and significant inhibition by suramine and high concentration of sodium azides (5 mM). According to similar apparent K-m values for both substrates, the ATP/ADP hydrolysis ratio, and Chevillard competition plot, NTPDase1 is dominant ATP/ADP hydrolyzing enzyme in myometrial cell membranes. RT-PCR analysis revealed expression of three members of ectonucleoside triphosphate diphosphohydrolase family (NTPDase 1, 2, and 8) in rat uterus. These findings may further elucidate the role of NTPDases and ATP in reproductive physiology.
T2  - Molecular and Cellular Biochemistry
T1  - ATP and ADP hydrolysis in cell membranes from rat myometrium
VL  - 371
IS  - 1-2
SP  - 199
EP  - 208
DO  - 10.1007/s11010-012-1436-2
ER  - 

@article{
author = "Milošević, Maja and Petrovic, Snjezana and Velickovic, Natasa and Grković, Ivana and Ignjatović, Marija and Horvat, Anica",
year = "2012",
abstract = "Extracellular nucleotides affect female reproductive functions, fertilization, and pregnancy. The aim of this study was to investigate biochemical characteristics of ATP and ADP hydrolysis and identify E-NTPDases in myometrial cell membranes from Wistar albino rats. The apparent K (m) values were 506.4 +/- A 62.1 and 638.8 +/- A 31.3 mu M, with a calculated V (max) (app) of 3,973.0 +/- A 279.5 and 2,853.9 +/- A 79.8 nmol/min/mg for ATP and ADP, respectively. The enzyme activity described here has common properties characteristic for NTPDases: divalent cation dependence; alkaline pH optimum for both substrates, insensitivity to some of classical ATPase inhibitors (ouabain, oligomycine, theophylline, levamisole) and significant inhibition by suramine and high concentration of sodium azides (5 mM). According to similar apparent K-m values for both substrates, the ATP/ADP hydrolysis ratio, and Chevillard competition plot, NTPDase1 is dominant ATP/ADP hydrolyzing enzyme in myometrial cell membranes. RT-PCR analysis revealed expression of three members of ectonucleoside triphosphate diphosphohydrolase family (NTPDase 1, 2, and 8) in rat uterus. These findings may further elucidate the role of NTPDases and ATP in reproductive physiology.",
journal = "Molecular and Cellular Biochemistry",
title = "ATP and ADP hydrolysis in cell membranes from rat myometrium",
volume = "371",
number = "1-2",
pages = "199-208",
doi = "10.1007/s11010-012-1436-2"
}

Milošević, M., Petrovic, S., Velickovic, N., Grković, I., Ignjatović, M.,& Horvat, A.. (2012). ATP and ADP hydrolysis in cell membranes from rat myometrium. in Molecular and Cellular Biochemistry, 371(1-2), 199-208.
https://doi.org/10.1007/s11010-012-1436-2

Milošević M, Petrovic S, Velickovic N, Grković I, Ignjatović M, Horvat A. ATP and ADP hydrolysis in cell membranes from rat myometrium. in Molecular and Cellular Biochemistry. 2012;371(1-2):199-208.
doi:10.1007/s11010-012-1436-2 .

Milošević, Maja, Petrovic, Snjezana, Velickovic, Natasa, Grković, Ivana, Ignjatović, Marija, Horvat, Anica, "ATP and ADP hydrolysis in cell membranes from rat myometrium" in Molecular and Cellular Biochemistry, 371, no. 1-2 (2012):199-208,
https://doi.org/10.1007/s11010-012-1436-2 . .

TY  - CONF
AU  - Drakulić, Dunja R.
AU  - Grković, Ivana
AU  - Milošević, Maja
AU  - Petrović, Snježana
AU  - Stanojlović, Miloš R.
AU  - Mitrović, Nataša Lj.
AU  - Horvat, Anica
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9295
AB  - The present study was performed to investigate whether acute whole-body exposure of
female adult rats to low dose (0.5 Gy) of ionizing irradiation (IR) is sufficient to alter
ectonucleotidase enzyme activities in the brain. All measurements were done at time
points 1, 24 and 72h after irradiation. Neuronal synaptic plasma membranes (SPMs)
were isolated from whole brains and enzyme activities were determined by monitoring
ATP, ADP and AMP hydrolysis in vitro. Our results indicate that whole-body IR is
able to modulate investigated brain enzyme activities in a time-dependent manner.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry
T1  - Radiation-mediated modulations of extracelluar nucleotide hydrolysis in adult female rat brain
VL  - 1
SP  - 373
EP  - 375
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9295
ER  - 

@conference{
author = "Drakulić, Dunja R. and Grković, Ivana and Milošević, Maja and Petrović, Snježana and Stanojlović, Miloš R. and Mitrović, Nataša Lj. and Horvat, Anica",
year = "2012",
abstract = "The present study was performed to investigate whether acute whole-body exposure of
female adult rats to low dose (0.5 Gy) of ionizing irradiation (IR) is sufficient to alter
ectonucleotidase enzyme activities in the brain. All measurements were done at time
points 1, 24 and 72h after irradiation. Neuronal synaptic plasma membranes (SPMs)
were isolated from whole brains and enzyme activities were determined by monitoring
ATP, ADP and AMP hydrolysis in vitro. Our results indicate that whole-body IR is
able to modulate investigated brain enzyme activities in a time-dependent manner.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry",
title = "Radiation-mediated modulations of extracelluar nucleotide hydrolysis in adult female rat brain",
volume = "1",
pages = "373-375",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9295"
}

Drakulić, D. R., Grković, I., Milošević, M., Petrović, S., Stanojlović, M. R., Mitrović, N. Lj.,& Horvat, A.. (2012). Radiation-mediated modulations of extracelluar nucleotide hydrolysis in adult female rat brain. in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 1, 373-375.
https://hdl.handle.net/21.15107/rcub_vinar_9295

Drakulić DR, Grković I, Milošević M, Petrović S, Stanojlović MR, Mitrović NL, Horvat A. Radiation-mediated modulations of extracelluar nucleotide hydrolysis in adult female rat brain. in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry. 2012;1:373-375.
https://hdl.handle.net/21.15107/rcub_vinar_9295 .

Drakulić, Dunja R., Grković, Ivana, Milošević, Maja, Petrović, Snježana, Stanojlović, Miloš R., Mitrović, Nataša Lj., Horvat, Anica, "Radiation-mediated modulations of extracelluar nucleotide hydrolysis in adult female rat brain" in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry, 1 (2012):373-375,
https://hdl.handle.net/21.15107/rcub_vinar_9295 .

TY  - CONF
AU  - Stanojlović, Miloš R.
AU  - Drakulić, Dunja R.
AU  - Grković, Ivana
AU  - Mitrović, Nataša Lj.
AU  - Horvat, Anica
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9296
AB  - Model of permanent bilateral occlusion of common carotid arteries (2VO) is generally
used to investigate mechanisms of chronic cerebral hypoperfusion that occurs in aging
and other neurodegenerative processes. The aim of this study was to determine timedependent modulation of mitochondrial apoptotic signaling in cortical brain area
following chronic cerebral hypoperfusion. Using Western blot technique we monitored
the changes in the expression of proteins of Bcl-2 family (Bcl-2, Bax) 3, 7 and 90 days
following the insult. According to our results the greatest impact of chronic cerebral
hipoperfusion occurred on 7th day.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry
T1  - Time-dependent expression of Bcl-2 and Bax proteins in cortical brain area of adult wistar rats after permanent bilateral occlusions
VL  - 1
SP  - 376
EP  - 378
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9296
ER  - 

@conference{
author = "Stanojlović, Miloš R. and Drakulić, Dunja R. and Grković, Ivana and Mitrović, Nataša Lj. and Horvat, Anica",
year = "2012",
abstract = "Model of permanent bilateral occlusion of common carotid arteries (2VO) is generally
used to investigate mechanisms of chronic cerebral hypoperfusion that occurs in aging
and other neurodegenerative processes. The aim of this study was to determine timedependent modulation of mitochondrial apoptotic signaling in cortical brain area
following chronic cerebral hypoperfusion. Using Western blot technique we monitored
the changes in the expression of proteins of Bcl-2 family (Bcl-2, Bax) 3, 7 and 90 days
following the insult. According to our results the greatest impact of chronic cerebral
hipoperfusion occurred on 7th day.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry",
title = "Time-dependent expression of Bcl-2 and Bax proteins in cortical brain area of adult wistar rats after permanent bilateral occlusions",
volume = "1",
pages = "376-378",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9296"
}

Stanojlović, M. R., Drakulić, D. R., Grković, I., Mitrović, N. Lj.,& Horvat, A.. (2012). Time-dependent expression of Bcl-2 and Bax proteins in cortical brain area of adult wistar rats after permanent bilateral occlusions. in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 1, 376-378.
https://hdl.handle.net/21.15107/rcub_vinar_9296

Stanojlović MR, Drakulić DR, Grković I, Mitrović NL, Horvat A. Time-dependent expression of Bcl-2 and Bax proteins in cortical brain area of adult wistar rats after permanent bilateral occlusions. in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry. 2012;1:376-378.
https://hdl.handle.net/21.15107/rcub_vinar_9296 .

Stanojlović, Miloš R., Drakulić, Dunja R., Grković, Ivana, Mitrović, Nataša Lj., Horvat, Anica, "Time-dependent expression of Bcl-2 and Bax proteins in cortical brain area of adult wistar rats after permanent bilateral occlusions" in Physical chemistry 2012 : 11th international conference on fundamental and applied aspects of physical chemistry, 1 (2012):376-378,
https://hdl.handle.net/21.15107/rcub_vinar_9296 .

TY  - JOUR
AU  - Petrović, S.
AU  - Velickovic, N.
AU  - Stanojević, Ivana
AU  - Milošević, Maja
AU  - Drakulić, Dunja R.
AU  - Stanojlović, Miloš R.
AU  - Horvat, Anica
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4512
AB  - Our results, as well as those of others, have indicated that 17 beta-estradiol (E2) exerts its nongenomic effects in neuronal cells by affecting plasma membrane Ca2+ flux. In neuronal cells mitochondria possess Ca2+ buffering properties as they both sequester and release Ca2+. The goal of this study was to examine the rapid non-genomic effect of E2 on mitochondria! Ca2+ transport in hippocampal synaptosomes from ovariectomised rats. In addition, we aimed to determine if, and to what extent, E2 receptors participated in mitochondria! Ca2+ transport modulation by E2 in vitro. E2-specific binding and Ca2+ transport was monitored. At physiological E2 concentrations (0.1-1.5 nmol/L), specific E2 binding to mitochondria isolated from hippocampal synaptosomes was detected with a B-max and K-m of 37.6 +/- 2.6 fmol/mg protein and 0.69 +/- 0.14 nmol/L of free E2, respectively. The main mitochondrial Ca2+ influx mechanism is the Ruthenium Red-sensitive uniporter driven by mitochondrial membrane potential. Despite no effect of E2 on Ca2+ influx, a physiological E2 concentration (0.5 nmol/L) protected mitochondrial membrane potential and consequently Ca2+ influx from the uncoupling agent carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (1 mu mol/L). In neuronal cells the predominant mitochondria! Ca2+ efflux mechanism is the Na+/Ca2+ exchanger. E2 caused Ca2+ efflux inhibition (by 46%) coupled with increased affinity of the Na+/Ca2+ exchanger for Na+. Using E2 receptor (ER alpha and ER beta) antagonists and agonists, we confirmed ER betas involvement in E2-induced mitochondrial membrane potential protection as well as Ca2+ efflux inhibition. In summary, our results indicate that the nongenomic neuromodulatory role of E2 in rat hippocampus is achieved by affecting mitochondria! Ca2+ transport via, in part, mitochondrial ER beta. (C) 2011 IBRO. Published by Elsevier Ltd. All rights reserved.
T2  - Neuroscience
T1  - Inhibition of Mitochondrial Na+-Dependent Ca2+ Efflux By 17 Beta-Estradiol in the Rat Hippocampus
VL  - 192
SP  - 195
EP  - 204
DO  - 10.1016/j.neuroscience.2011.06.030
ER  - 

@article{
author = "Petrović, S. and Velickovic, N. and Stanojević, Ivana and Milošević, Maja and Drakulić, Dunja R. and Stanojlović, Miloš R. and Horvat, Anica",
year = "2011",
abstract = "Our results, as well as those of others, have indicated that 17 beta-estradiol (E2) exerts its nongenomic effects in neuronal cells by affecting plasma membrane Ca2+ flux. In neuronal cells mitochondria possess Ca2+ buffering properties as they both sequester and release Ca2+. The goal of this study was to examine the rapid non-genomic effect of E2 on mitochondria! Ca2+ transport in hippocampal synaptosomes from ovariectomised rats. In addition, we aimed to determine if, and to what extent, E2 receptors participated in mitochondria! Ca2+ transport modulation by E2 in vitro. E2-specific binding and Ca2+ transport was monitored. At physiological E2 concentrations (0.1-1.5 nmol/L), specific E2 binding to mitochondria isolated from hippocampal synaptosomes was detected with a B-max and K-m of 37.6 +/- 2.6 fmol/mg protein and 0.69 +/- 0.14 nmol/L of free E2, respectively. The main mitochondrial Ca2+ influx mechanism is the Ruthenium Red-sensitive uniporter driven by mitochondrial membrane potential. Despite no effect of E2 on Ca2+ influx, a physiological E2 concentration (0.5 nmol/L) protected mitochondrial membrane potential and consequently Ca2+ influx from the uncoupling agent carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (1 mu mol/L). In neuronal cells the predominant mitochondria! Ca2+ efflux mechanism is the Na+/Ca2+ exchanger. E2 caused Ca2+ efflux inhibition (by 46%) coupled with increased affinity of the Na+/Ca2+ exchanger for Na+. Using E2 receptor (ER alpha and ER beta) antagonists and agonists, we confirmed ER betas involvement in E2-induced mitochondrial membrane potential protection as well as Ca2+ efflux inhibition. In summary, our results indicate that the nongenomic neuromodulatory role of E2 in rat hippocampus is achieved by affecting mitochondria! Ca2+ transport via, in part, mitochondrial ER beta. (C) 2011 IBRO. Published by Elsevier Ltd. All rights reserved.",
journal = "Neuroscience",
title = "Inhibition of Mitochondrial Na+-Dependent Ca2+ Efflux By 17 Beta-Estradiol in the Rat Hippocampus",
volume = "192",
pages = "195-204",
doi = "10.1016/j.neuroscience.2011.06.030"
}

Petrović, S., Velickovic, N., Stanojević, I., Milošević, M., Drakulić, D. R., Stanojlović, M. R.,& Horvat, A.. (2011). Inhibition of Mitochondrial Na+-Dependent Ca2+ Efflux By 17 Beta-Estradiol in the Rat Hippocampus. in Neuroscience, 192, 195-204.
https://doi.org/10.1016/j.neuroscience.2011.06.030

Petrović S, Velickovic N, Stanojević I, Milošević M, Drakulić DR, Stanojlović MR, Horvat A. Inhibition of Mitochondrial Na+-Dependent Ca2+ Efflux By 17 Beta-Estradiol in the Rat Hippocampus. in Neuroscience. 2011;192:195-204.
doi:10.1016/j.neuroscience.2011.06.030 .

Petrović, S., Velickovic, N., Stanojević, Ivana, Milošević, Maja, Drakulić, Dunja R., Stanojlović, Miloš R., Horvat, Anica, "Inhibition of Mitochondrial Na+-Dependent Ca2+ Efflux By 17 Beta-Estradiol in the Rat Hippocampus" in Neuroscience, 192 (2011):195-204,
https://doi.org/10.1016/j.neuroscience.2011.06.030 . .

TY  - JOUR
AU  - Stanojević, Ivana
AU  - Drakulić, Dunja R.
AU  - Petrovic, S.
AU  - Milošević, Maja
AU  - Jovanović, N.
AU  - Horvat, Anica
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4603
AB  - A family of enzymes named ecto-nucleoside triphosphate diphosphohydrolase (NTPDases) catalyzes the termination of ATP and ADP actions. Three different NTPDases (NTPDase 1-3), differing in their preference for a substrate, have been localized in the brain of adult mammals. The goal of our study was to clarify ATP and ADP hydrolyzing activities and kinetic parameters of NTPDases in synaptic plasma membranes (SPM) isolated from 15-, 30-, 60- and 90-days-old female rat brains. ATP and ADP hydrolysis were maximal in the presence of Mg(2+) and showed insensitivity to ion-transporting ATPase inhibitors. The pronounced increase in both, ATP and ADP hydrolysis, were found in the SPM isolated from rats in the first month of life, stayed at the same level in the second month, and then decreased in adulthood. Kinetic analysis are also developmental-dependent, and together with the rate of ATP:ADP hydrolysis, point that all three NTPDases are present in SPM isolated from different developmental stages, with different, developmental-dependent proportion of activities. The lowest velocity and the highest affinity were observed for ATP hydrolyses, while the highest velocity and lowest affinity were detected for ADP hydrolyses in SPM isolated from 15-day old rats. Since specific ATP and ADP hydrolysis were lowest in this stage, we concluded that velocity is crucial for ATPase-, while affinity is for ADPase-part of NTPDases. Increased NTPDases activities, changes in their hydrolysis velocity and substrates affinities during rat postnatal development indicate involvement of adenine nucleotides in processes implicated to neuronal maturation and augmented neuroprotection.
T2  - Russian Journal of Physical Chemistry A
T1  - Kinetic characterization of ecto-nucleoside triphosphate diphosphohydrolases in brain nerve terminals during rat postnatal development
VL  - 85
IS  - 13
SP  - 2416
EP  - 2421
DO  - 10.1134/S0036024411130292
ER  - 

@article{
author = "Stanojević, Ivana and Drakulić, Dunja R. and Petrovic, S. and Milošević, Maja and Jovanović, N. and Horvat, Anica",
year = "2011",
abstract = "A family of enzymes named ecto-nucleoside triphosphate diphosphohydrolase (NTPDases) catalyzes the termination of ATP and ADP actions. Three different NTPDases (NTPDase 1-3), differing in their preference for a substrate, have been localized in the brain of adult mammals. The goal of our study was to clarify ATP and ADP hydrolyzing activities and kinetic parameters of NTPDases in synaptic plasma membranes (SPM) isolated from 15-, 30-, 60- and 90-days-old female rat brains. ATP and ADP hydrolysis were maximal in the presence of Mg(2+) and showed insensitivity to ion-transporting ATPase inhibitors. The pronounced increase in both, ATP and ADP hydrolysis, were found in the SPM isolated from rats in the first month of life, stayed at the same level in the second month, and then decreased in adulthood. Kinetic analysis are also developmental-dependent, and together with the rate of ATP:ADP hydrolysis, point that all three NTPDases are present in SPM isolated from different developmental stages, with different, developmental-dependent proportion of activities. The lowest velocity and the highest affinity were observed for ATP hydrolyses, while the highest velocity and lowest affinity were detected for ADP hydrolyses in SPM isolated from 15-day old rats. Since specific ATP and ADP hydrolysis were lowest in this stage, we concluded that velocity is crucial for ATPase-, while affinity is for ADPase-part of NTPDases. Increased NTPDases activities, changes in their hydrolysis velocity and substrates affinities during rat postnatal development indicate involvement of adenine nucleotides in processes implicated to neuronal maturation and augmented neuroprotection.",
journal = "Russian Journal of Physical Chemistry A",
title = "Kinetic characterization of ecto-nucleoside triphosphate diphosphohydrolases in brain nerve terminals during rat postnatal development",
volume = "85",
number = "13",
pages = "2416-2421",
doi = "10.1134/S0036024411130292"
}

Stanojević, I., Drakulić, D. R., Petrovic, S., Milošević, M., Jovanović, N.,& Horvat, A.. (2011). Kinetic characterization of ecto-nucleoside triphosphate diphosphohydrolases in brain nerve terminals during rat postnatal development. in Russian Journal of Physical Chemistry A, 85(13), 2416-2421.
https://doi.org/10.1134/S0036024411130292

Stanojević I, Drakulić DR, Petrovic S, Milošević M, Jovanović N, Horvat A. Kinetic characterization of ecto-nucleoside triphosphate diphosphohydrolases in brain nerve terminals during rat postnatal development. in Russian Journal of Physical Chemistry A. 2011;85(13):2416-2421.
doi:10.1134/S0036024411130292 .

Stanojević, Ivana, Drakulić, Dunja R., Petrovic, S., Milošević, Maja, Jovanović, N., Horvat, Anica, "Kinetic characterization of ecto-nucleoside triphosphate diphosphohydrolases in brain nerve terminals during rat postnatal development" in Russian Journal of Physical Chemistry A, 85, no. 13 (2011):2416-2421,
https://doi.org/10.1134/S0036024411130292 . .

TY  - JOUR
AU  - Stanojević, Ivana
AU  - Bjelobaba, Ivana
AU  - Nedeljković, Nadežda
AU  - Drakulić, Dunja R.
AU  - Petrović, Snježana
AU  - Stojiljković, Mirjana
AU  - Horvat, Anica
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4376
AB  - Ecto-5-nucleotidase (CD73; EC 3.1.3.5, e-5NT) is regarded as the key enzyme in the extracellular formation of adenosine, which acts as a neuromodulator and important trophic and homeostatic factor in the brain. In the present study, we have investigated e-5NT activity, kinetic properties concerning AMP hydrolysis and the enzyme protein abundance in the purified synaptic plasma membrane (SPM) preparations isolated from whole female rat brain at different ages. We observed pronounced increase in AMP hydrolyzing activity in SPM during maturation, with greatest increment between juvenile (15-day-old) and pre-pubertal (30-day-old) rats. Immunodetection of e-5NT protein in the SPM displayed the reverse pattern of expression, with the maximum relative abundance at juvenile and minimum relative abundance in the adult stage. Negative correlation between the enzyme activity and the enzyme protein abundance in the SPM indicates that e-5NT has additional roles in the synaptic compartment during postnatal brain development, other than those related to AMP hydrolysis. Determination of kinetic parameters, K(m) and V(max), suggested that the increase in the enzyme activity with maturation was entirely due to the increase in the enzyme catalytic efficiency (V(max)/K(m)). Finally, double immunofluorescence staining against e-5NT and presynaptic membrane marker syntaxin provided first direct evidence for the existence of this ecto-enzyme in the presynaptic compartment. The results of the study suggest that e-5NT may be a part of general scheme of brain development and synapse maturation and provide rationale for the previously reported inconsistencies between enzyme immunohistochemical and biochemical studies concerning localization of e-5NT in the brain. (C) 2011 ISDN. Published by Elsevier Ltd. All rights reserved.
T2  - International Journal of Developmental Neuroscience
T1  - Ontogenetic profile of ecto-5 -nucleotidase in rat brain synaptic plasma membranes
VL  - 29
IS  - 4
SP  - 397
EP  - 403
DO  - 10.1016/j.ijdevneu.2011.03.003
ER  - 

@article{
author = "Stanojević, Ivana and Bjelobaba, Ivana and Nedeljković, Nadežda and Drakulić, Dunja R. and Petrović, Snježana and Stojiljković, Mirjana and Horvat, Anica",
year = "2011",
abstract = "Ecto-5-nucleotidase (CD73; EC 3.1.3.5, e-5NT) is regarded as the key enzyme in the extracellular formation of adenosine, which acts as a neuromodulator and important trophic and homeostatic factor in the brain. In the present study, we have investigated e-5NT activity, kinetic properties concerning AMP hydrolysis and the enzyme protein abundance in the purified synaptic plasma membrane (SPM) preparations isolated from whole female rat brain at different ages. We observed pronounced increase in AMP hydrolyzing activity in SPM during maturation, with greatest increment between juvenile (15-day-old) and pre-pubertal (30-day-old) rats. Immunodetection of e-5NT protein in the SPM displayed the reverse pattern of expression, with the maximum relative abundance at juvenile and minimum relative abundance in the adult stage. Negative correlation between the enzyme activity and the enzyme protein abundance in the SPM indicates that e-5NT has additional roles in the synaptic compartment during postnatal brain development, other than those related to AMP hydrolysis. Determination of kinetic parameters, K(m) and V(max), suggested that the increase in the enzyme activity with maturation was entirely due to the increase in the enzyme catalytic efficiency (V(max)/K(m)). Finally, double immunofluorescence staining against e-5NT and presynaptic membrane marker syntaxin provided first direct evidence for the existence of this ecto-enzyme in the presynaptic compartment. The results of the study suggest that e-5NT may be a part of general scheme of brain development and synapse maturation and provide rationale for the previously reported inconsistencies between enzyme immunohistochemical and biochemical studies concerning localization of e-5NT in the brain. (C) 2011 ISDN. Published by Elsevier Ltd. All rights reserved.",
journal = "International Journal of Developmental Neuroscience",
title = "Ontogenetic profile of ecto-5 -nucleotidase in rat brain synaptic plasma membranes",
volume = "29",
number = "4",
pages = "397-403",
doi = "10.1016/j.ijdevneu.2011.03.003"
}

Stanojević, I., Bjelobaba, I., Nedeljković, N., Drakulić, D. R., Petrović, S., Stojiljković, M.,& Horvat, A.. (2011). Ontogenetic profile of ecto-5 -nucleotidase in rat brain synaptic plasma membranes. in International Journal of Developmental Neuroscience, 29(4), 397-403.
https://doi.org/10.1016/j.ijdevneu.2011.03.003

Stanojević I, Bjelobaba I, Nedeljković N, Drakulić DR, Petrović S, Stojiljković M, Horvat A. Ontogenetic profile of ecto-5 -nucleotidase in rat brain synaptic plasma membranes. in International Journal of Developmental Neuroscience. 2011;29(4):397-403.
doi:10.1016/j.ijdevneu.2011.03.003 .

Stanojević, Ivana, Bjelobaba, Ivana, Nedeljković, Nadežda, Drakulić, Dunja R., Petrović, Snježana, Stojiljković, Mirjana, Horvat, Anica, "Ontogenetic profile of ecto-5 -nucleotidase in rat brain synaptic plasma membranes" in International Journal of Developmental Neuroscience, 29, no. 4 (2011):397-403,
https://doi.org/10.1016/j.ijdevneu.2011.03.003 . .

TY  - JOUR
AU  - Horvat, Anica
AU  - Stanojević, Ivana
AU  - Drakulić, Dunja R.
AU  - Velickovic, Natasa
AU  - Petrović, Snježana
AU  - Milošević, Maja
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3941
AB  - The aim of the present study was to examine the effect of acute restraint stress on rat brain synaptosomal plasma membrane (SPM) ecto-nucleotidase activities at specific stages of postnatal development (15-, 30-, 60- and 90-day-old rats) by measuring the rates of ATP, ADP and AMP hydrolysis 1,24 and 72 h post-stress. At 1 h after stress NTPDase and ecto-5-nucleotidase activities were decreased in rats aged up to 60 days old. In adult rats elevated enzyme activities were detected, which indicated the existence of different short-term stress responses during development. A similar pattern of ATP and ADP hydrolysis changes as well as the ATP/ADP ratio in all developmental stages indicated that NTPDase3 was acutely affected after stress. The long-term effect of acute stress on NTPDase activity differed during postnatal development. In juvenile animals (15 days old) NTPDase activity was not altered. However, in later developmental stages (30 and 60 days old rats) NTPDase activity decreased and persisted for 72 h post-stress. In adult rats only ATP hydrolysis was decreased after 24 h, indicating that ecto-ATPase was affected by stress. Ecto-5-nucleotidase hydrolysing activity was decreased within 24 h in adult rats, while in 15- and 30-day old rats it decreased 72 h post-stress. At equivalent times in pubertal rats (60 days old) a slight activation of ecto-5-nucleotidase was detected. Our results highlight the developmental-dependence of brain ecto-nucleotidase susceptibility to acute stress and the likely existence of different mechanisms involved in time-dependent ecto-nucleotidase activity modulation following stress exposure. Clearly there are differences in the response of the purinergic system to acute restraint stress between young and adult rats. (C) 2009 ISDN. Published by Elsevier Ltd. All rights reserved.
T2  - International Journal of Developmental Neuroscience
T1  - Effect of acute stress on NTPDase and 5 -nucleotidase activities in brain synaptosomes in different stages of development
VL  - 28
IS  - 2
SP  - 175
EP  - 182
DO  - 10.1016/j.ijdevneu.2009.11.005
ER  - 

@article{
author = "Horvat, Anica and Stanojević, Ivana and Drakulić, Dunja R. and Velickovic, Natasa and Petrović, Snježana and Milošević, Maja",
year = "2010",
abstract = "The aim of the present study was to examine the effect of acute restraint stress on rat brain synaptosomal plasma membrane (SPM) ecto-nucleotidase activities at specific stages of postnatal development (15-, 30-, 60- and 90-day-old rats) by measuring the rates of ATP, ADP and AMP hydrolysis 1,24 and 72 h post-stress. At 1 h after stress NTPDase and ecto-5-nucleotidase activities were decreased in rats aged up to 60 days old. In adult rats elevated enzyme activities were detected, which indicated the existence of different short-term stress responses during development. A similar pattern of ATP and ADP hydrolysis changes as well as the ATP/ADP ratio in all developmental stages indicated that NTPDase3 was acutely affected after stress. The long-term effect of acute stress on NTPDase activity differed during postnatal development. In juvenile animals (15 days old) NTPDase activity was not altered. However, in later developmental stages (30 and 60 days old rats) NTPDase activity decreased and persisted for 72 h post-stress. In adult rats only ATP hydrolysis was decreased after 24 h, indicating that ecto-ATPase was affected by stress. Ecto-5-nucleotidase hydrolysing activity was decreased within 24 h in adult rats, while in 15- and 30-day old rats it decreased 72 h post-stress. At equivalent times in pubertal rats (60 days old) a slight activation of ecto-5-nucleotidase was detected. Our results highlight the developmental-dependence of brain ecto-nucleotidase susceptibility to acute stress and the likely existence of different mechanisms involved in time-dependent ecto-nucleotidase activity modulation following stress exposure. Clearly there are differences in the response of the purinergic system to acute restraint stress between young and adult rats. (C) 2009 ISDN. Published by Elsevier Ltd. All rights reserved.",
journal = "International Journal of Developmental Neuroscience",
title = "Effect of acute stress on NTPDase and 5 -nucleotidase activities in brain synaptosomes in different stages of development",
volume = "28",
number = "2",
pages = "175-182",
doi = "10.1016/j.ijdevneu.2009.11.005"
}

Horvat, A., Stanojević, I., Drakulić, D. R., Velickovic, N., Petrović, S.,& Milošević, M.. (2010). Effect of acute stress on NTPDase and 5 -nucleotidase activities in brain synaptosomes in different stages of development. in International Journal of Developmental Neuroscience, 28(2), 175-182.
https://doi.org/10.1016/j.ijdevneu.2009.11.005

Horvat A, Stanojević I, Drakulić DR, Velickovic N, Petrović S, Milošević M. Effect of acute stress on NTPDase and 5 -nucleotidase activities in brain synaptosomes in different stages of development. in International Journal of Developmental Neuroscience. 2010;28(2):175-182.
doi:10.1016/j.ijdevneu.2009.11.005 .

Horvat, Anica, Stanojević, Ivana, Drakulić, Dunja R., Velickovic, Natasa, Petrović, Snježana, Milošević, Maja, "Effect of acute stress on NTPDase and 5 -nucleotidase activities in brain synaptosomes in different stages of development" in International Journal of Developmental Neuroscience, 28, no. 2 (2010):175-182,
https://doi.org/10.1016/j.ijdevneu.2009.11.005 . .

TY  - CONF
AU  - Milošević, Maja
AU  - Drakulić, Dunja R.
AU  - Petrović, Snježana
AU  - Stanojević, Ivana
AU  - Veličković, Nataša
AU  - Horvat, Anica
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9319
AB  - L-cysteine is used as effective oral chelating agent due to property of its sulfhydryl
group to bind heavy metal ions. The aim of this study was to investigate ability of
L-cysteine to prevent mercury (II) and cadmium (II) induced ecto-ATPase activity
inhibition of rat uterus plasma membranes. Results show that 10 mmol/l L-cysteine
have protective effect on enzyme activity in the presence of cadmium and mercury
ions.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
T1  - L-cysteine modulates the ecto-atpase activity inhibition in presence of cadmium (ii) and mercury (ii) ions
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9319
ER  - 

@conference{
author = "Milošević, Maja and Drakulić, Dunja R. and Petrović, Snježana and Stanojević, Ivana and Veličković, Nataša and Horvat, Anica",
year = "2010",
abstract = "L-cysteine is used as effective oral chelating agent due to property of its sulfhydryl
group to bind heavy metal ions. The aim of this study was to investigate ability of
L-cysteine to prevent mercury (II) and cadmium (II) induced ecto-ATPase activity
inhibition of rat uterus plasma membranes. Results show that 10 mmol/l L-cysteine
have protective effect on enzyme activity in the presence of cadmium and mercury
ions.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry",
title = "L-cysteine modulates the ecto-atpase activity inhibition in presence of cadmium (ii) and mercury (ii) ions",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9319"
}

Milošević, M., Drakulić, D. R., Petrović, S., Stanojević, I., Veličković, N.,& Horvat, A.. (2010). L-cysteine modulates the ecto-atpase activity inhibition in presence of cadmium (ii) and mercury (ii) ions. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia..
https://hdl.handle.net/21.15107/rcub_vinar_9319

Milošević M, Drakulić DR, Petrović S, Stanojević I, Veličković N, Horvat A. L-cysteine modulates the ecto-atpase activity inhibition in presence of cadmium (ii) and mercury (ii) ions. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry. 2010;.
https://hdl.handle.net/21.15107/rcub_vinar_9319 .

Milošević, Maja, Drakulić, Dunja R., Petrović, Snježana, Stanojević, Ivana, Veličković, Nataša, Horvat, Anica, "L-cysteine modulates the ecto-atpase activity inhibition in presence of cadmium (ii) and mercury (ii) ions" in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry (2010),
https://hdl.handle.net/21.15107/rcub_vinar_9319 .

TY  - CONF
AU  - Petrović, Snježana
AU  - Milošević, Maja
AU  - Stanojević, Ivana
AU  - Drakulić, Dunja R.
AU  - Jovanović, N.
AU  - Veličković, Nataša
AU  - Horvat, Anica
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9321
AB  - In this study the Ca2+ ion flux modulation in the synaptosomal mitochondria
isolated from caudate nucleus (CN) of the ovariectomised rats was examined.
17 beta-estradiol (E2), E2-conjugated to bovine serum albumin (E-BSA), estradiol
receptor α (ERα) agonist 4,4',4''-(4-propyl-[1H]-pyrazole-1,3,5-triyl) trisphenol
(PPT), ERβ agonist 2,3-bis(4-hydroxyphenyl)-propionitrile (DNP) and ERα/β
antagonist 7α,17β-[9[(4,4,5,5,5-pentafluoropentyl)sulfinyl]nonyl]estra-1,3,5(10)-
triene-3,17-diol (ICI 182,780) were used. The Ca2+ efflux inhibition of about 27%
was detected in the presence of 0.5 nmol/l E2, and of about 20% in the case of
E-BSA. DNP (10 nmol/l) was as much potent Ca2+ efflux inhibitor as E2, while
PPT (10 nmol/l) hardly had any inhibitory effect (9% efflux decrease). When E2
binding to ERα and ERβ was prevented by 1 μmol/l ICI 182,780, the Ca2+ efflux
inhibition of about 15% was detected. Our results suggest that E2 prevents Ca2+
efflux from synaptosomal mitochondria due to ERβ activation rather than ERα.
The involvement of the external E2 binding site on the mitochondrial membrane
probably different from ERα/β should not be excluded because of Ca2+ efflux
inhibition detected in the presence of E-BSA and ICI 182,780. The Ca2+ efflux
modulation could be the mechanism through which E2 exerts its neuromodulatory
role in specific brain structures.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
T1  - Na+-dependent Ca2+ ion flux inhibition by 17 beta-estradiol in caudate nucleus mitochondria
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9321
ER  - 

@conference{
author = "Petrović, Snježana and Milošević, Maja and Stanojević, Ivana and Drakulić, Dunja R. and Jovanović, N. and Veličković, Nataša and Horvat, Anica",
year = "2010",
abstract = "In this study the Ca2+ ion flux modulation in the synaptosomal mitochondria
isolated from caudate nucleus (CN) of the ovariectomised rats was examined.
17 beta-estradiol (E2), E2-conjugated to bovine serum albumin (E-BSA), estradiol
receptor α (ERα) agonist 4,4',4''-(4-propyl-[1H]-pyrazole-1,3,5-triyl) trisphenol
(PPT), ERβ agonist 2,3-bis(4-hydroxyphenyl)-propionitrile (DNP) and ERα/β
antagonist 7α,17β-[9[(4,4,5,5,5-pentafluoropentyl)sulfinyl]nonyl]estra-1,3,5(10)-
triene-3,17-diol (ICI 182,780) were used. The Ca2+ efflux inhibition of about 27%
was detected in the presence of 0.5 nmol/l E2, and of about 20% in the case of
E-BSA. DNP (10 nmol/l) was as much potent Ca2+ efflux inhibitor as E2, while
PPT (10 nmol/l) hardly had any inhibitory effect (9% efflux decrease). When E2
binding to ERα and ERβ was prevented by 1 μmol/l ICI 182,780, the Ca2+ efflux
inhibition of about 15% was detected. Our results suggest that E2 prevents Ca2+
efflux from synaptosomal mitochondria due to ERβ activation rather than ERα.
The involvement of the external E2 binding site on the mitochondrial membrane
probably different from ERα/β should not be excluded because of Ca2+ efflux
inhibition detected in the presence of E-BSA and ICI 182,780. The Ca2+ efflux
modulation could be the mechanism through which E2 exerts its neuromodulatory
role in specific brain structures.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry",
title = "Na+-dependent Ca2+ ion flux inhibition by 17 beta-estradiol in caudate nucleus mitochondria",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9321"
}

Petrović, S., Milošević, M., Stanojević, I., Drakulić, D. R., Jovanović, N., Veličković, N.,& Horvat, A.. (2010). Na+-dependent Ca2+ ion flux inhibition by 17 beta-estradiol in caudate nucleus mitochondria. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia..
https://hdl.handle.net/21.15107/rcub_vinar_9321

Petrović S, Milošević M, Stanojević I, Drakulić DR, Jovanović N, Veličković N, Horvat A. Na+-dependent Ca2+ ion flux inhibition by 17 beta-estradiol in caudate nucleus mitochondria. in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry. 2010;.
https://hdl.handle.net/21.15107/rcub_vinar_9321 .

Petrović, Snježana, Milošević, Maja, Stanojević, Ivana, Drakulić, Dunja R., Jovanović, N., Veličković, Nataša, Horvat, Anica, "Na+-dependent Ca2+ ion flux inhibition by 17 beta-estradiol in caudate nucleus mitochondria" in Physical chemistry 2010 : 10th international conference on fundamental and applied aspects of physical chemistry (2010),
https://hdl.handle.net/21.15107/rcub_vinar_9321 .