TY  - JOUR
AU  - Preljević, Kenan
AU  - Pašić, Ivana
AU  - Vlaović, Milorad
AU  - Matić, Ivana Z.
AU  - Krivokapić, Slađana
AU  - Petrović, Nina
AU  - Stanojković, Tatjana
AU  - Živković, Vladimir
AU  - Perović, Svetlana
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12935
AB  - The essential oils of Thymus vulgaris (TVEO) and Thymus serpyllum (TSEO) show different biological activities. The aim of the study was to evaluate the biological activities of TVEO and TSEO from Montenegro. The main components of TVEO were p-cymene (29.52%), thymol (22.8%) and linalool (4.73%) while the main components of TSEO were p-cymene (19.04%), geraniol (11,09%), linalool (9.16%), geranyl acetate (6.49%) and borneol (5.24%). Antioxidant activity determined via DPPH for TVEO was 4.49 and FRAP 1130.27, while for TSEO it was estimated that DPPH was 4.88 μL/mL and FRAP was 701.25 μmol FRAP/L. Both essential oils were active against all tested bacteria, with the highest level of sensitivity of E. coli with MIC of 1.5625 μL/mL. Essential oils showed strong cytotoxic effects on human cancer cell lines, with IC50 values ranging from 0.20 to 0.24 μL/mL for TVEO and from 0.32 to 0.49 μL/mL for TSEO. TVEO caused apoptosis in cervical adenocarcinoma HeLa cells through activation of caspase-3 and caspase-8, while TSEO caused apoptosis through caspase-3. EOs decreased levels of oxidative stress in normal MRC-5 cells. HeLa cells treated with TVEO had reduced MMP2 expression levels, while cells treated with TSEO had lowered MMP2 and MMP9 levels. The treatment of HeLa cells with TVEO increased the levels of miR-16 and miR-34a, indicating potential tumor-suppressive properties. Our findings suggest that Thymus essential oils may be considered as good candidates for further investigation as cancer-chemopreventive and cancer-therapeutic agents.
T2  - Fitoterapia
T1  - Comparative analysis of chemical profiles, antioxidant, antibacterial, and anticancer effects of essential oils of two Thymus species from Montenegro
VL  - 174
SP  - 105871
DO  - 10.1016/j.fitote.2024.105871
ER  - 

@article{
author = "Preljević, Kenan and Pašić, Ivana and Vlaović, Milorad and Matić, Ivana Z. and Krivokapić, Slađana and Petrović, Nina and Stanojković, Tatjana and Živković, Vladimir and Perović, Svetlana",
year = "2024",
abstract = "The essential oils of Thymus vulgaris (TVEO) and Thymus serpyllum (TSEO) show different biological activities. The aim of the study was to evaluate the biological activities of TVEO and TSEO from Montenegro. The main components of TVEO were p-cymene (29.52%), thymol (22.8%) and linalool (4.73%) while the main components of TSEO were p-cymene (19.04%), geraniol (11,09%), linalool (9.16%), geranyl acetate (6.49%) and borneol (5.24%). Antioxidant activity determined via DPPH for TVEO was 4.49 and FRAP 1130.27, while for TSEO it was estimated that DPPH was 4.88 μL/mL and FRAP was 701.25 μmol FRAP/L. Both essential oils were active against all tested bacteria, with the highest level of sensitivity of E. coli with MIC of 1.5625 μL/mL. Essential oils showed strong cytotoxic effects on human cancer cell lines, with IC50 values ranging from 0.20 to 0.24 μL/mL for TVEO and from 0.32 to 0.49 μL/mL for TSEO. TVEO caused apoptosis in cervical adenocarcinoma HeLa cells through activation of caspase-3 and caspase-8, while TSEO caused apoptosis through caspase-3. EOs decreased levels of oxidative stress in normal MRC-5 cells. HeLa cells treated with TVEO had reduced MMP2 expression levels, while cells treated with TSEO had lowered MMP2 and MMP9 levels. The treatment of HeLa cells with TVEO increased the levels of miR-16 and miR-34a, indicating potential tumor-suppressive properties. Our findings suggest that Thymus essential oils may be considered as good candidates for further investigation as cancer-chemopreventive and cancer-therapeutic agents.",
journal = "Fitoterapia",
title = "Comparative analysis of chemical profiles, antioxidant, antibacterial, and anticancer effects of essential oils of two Thymus species from Montenegro",
volume = "174",
pages = "105871",
doi = "10.1016/j.fitote.2024.105871"
}

Preljević, K., Pašić, I., Vlaović, M., Matić, I. Z., Krivokapić, S., Petrović, N., Stanojković, T., Živković, V.,& Perović, S.. (2024). Comparative analysis of chemical profiles, antioxidant, antibacterial, and anticancer effects of essential oils of two Thymus species from Montenegro. in Fitoterapia, 174, 105871.
https://doi.org/10.1016/j.fitote.2024.105871

Preljević K, Pašić I, Vlaović M, Matić IZ, Krivokapić S, Petrović N, Stanojković T, Živković V, Perović S. Comparative analysis of chemical profiles, antioxidant, antibacterial, and anticancer effects of essential oils of two Thymus species from Montenegro. in Fitoterapia. 2024;174:105871.
doi:10.1016/j.fitote.2024.105871 .

Preljević, Kenan, Pašić, Ivana, Vlaović, Milorad, Matić, Ivana Z., Krivokapić, Slađana, Petrović, Nina, Stanojković, Tatjana, Živković, Vladimir, Perović, Svetlana, "Comparative analysis of chemical profiles, antioxidant, antibacterial, and anticancer effects of essential oils of two Thymus species from Montenegro" in Fitoterapia, 174 (2024):105871,
https://doi.org/10.1016/j.fitote.2024.105871 . .

TY  - JOUR
AU  - Matić, Ivana Z.
AU  - Mraković, Ana
AU  - Rakočević, Zlatko
AU  - Stoiljković, Milovan
AU  - Pavlović, Vladimir B.
AU  - Momić, Tatjana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11544
AB  - Background: Although luteolin has been confirmed as potent anticancer agent, its potential application as therapeutic is limited by its water solubility. To overcome this shortcoming nanoparticle technology approach was applied. Owing to their proven low toxicity and the possibility to be easily functionalized gold nanoparticles (AuNP) were the nanosystem of choice used in this study. Novel luteolin capped gold nanoparticles (AuNPL) were synthesized and their anticancer effect towards human cervical adenocarcinoma HeLa cells was investigated in vitro. Methods: AuNPL were synthesized by reducing chloroauric acid by trisodium citrate with subsequent addition of luteoline during synthesis and their physicochemical characterization was done. AuNPL cytotoxicity against HeLa, human malignant melanoma A375, and normal human keratinocytes HaCaT cells was tested by MTT cell survival assay, and their IC50 values were determined. The capability of AuNPL to induce cell cycle arrest and apoptosis in HeLa cells were demonstrated by flow cytometry. The antioxidant activity of AuNPL was assessed by DPPH⋅ and ABTS⋅þ scavenging assays. Cytoprotective properties of AuNPL towards HaCaT cells were examined by measuring the physiological and H2O2 induced intracellular reactive oxygen species (ROS) levels using flow cytometry. Also, genotoxicity of AuNPL in HaCaT cells was investigated by the single cell alkaline comet assay. Results: Spherical AuNPL, stable in aqueous solution up to six months at 4 ◦C were obtained in the synthesis. The selectivity in the cytotoxic action of AuNPL on HeLa and A375 cancer cells compared with their cytotoxicity on normal keratinocytes HaCaT was observed. AuNPL exerted their cytotoxic activity against HeLa cells through accumulation of the cells in the subG1 phase of the cell cycle, inducing the apoptotic cell death mediated by the activation of caspase-3 − 8, and − 9. AuNPL antioxidative potential was confirmed by DPPH⋅ and ABTS⋅þ scavenging assays. IC50 concentration of AuNPL exerted cytoprotective effect against HaCaT cells by the significant reduction of the physiological intracellular ROS level. Additionally, AuNPL were shown as more cytoprotective towards HaCaT cells then luteolin due to the more successful elimination of H2O2 induced intracellular ROS. Moreover, nontoxic concentrations of AuNPL did not cause considerable DNA damage of HaCaT cells, indicating low genotoxicity of the nanoparticles.
T2  - Journal of Trace Elements in Medicine and Biology
T1  - Anticancer effect of novel luteolin capped gold nanoparticles selectively cytotoxic towards human cervical adenocarcinoma HeLa cells: An in vitro approach
VL  - 80
SP  - 127286
DO  - 10.1016/j.jtemb.2023.127286
ER  - 

@article{
author = "Matić, Ivana Z. and Mraković, Ana and Rakočević, Zlatko and Stoiljković, Milovan and Pavlović, Vladimir B. and Momić, Tatjana",
year = "2023",
abstract = "Background: Although luteolin has been confirmed as potent anticancer agent, its potential application as therapeutic is limited by its water solubility. To overcome this shortcoming nanoparticle technology approach was applied. Owing to their proven low toxicity and the possibility to be easily functionalized gold nanoparticles (AuNP) were the nanosystem of choice used in this study. Novel luteolin capped gold nanoparticles (AuNPL) were synthesized and their anticancer effect towards human cervical adenocarcinoma HeLa cells was investigated in vitro. Methods: AuNPL were synthesized by reducing chloroauric acid by trisodium citrate with subsequent addition of luteoline during synthesis and their physicochemical characterization was done. AuNPL cytotoxicity against HeLa, human malignant melanoma A375, and normal human keratinocytes HaCaT cells was tested by MTT cell survival assay, and their IC50 values were determined. The capability of AuNPL to induce cell cycle arrest and apoptosis in HeLa cells were demonstrated by flow cytometry. The antioxidant activity of AuNPL was assessed by DPPH⋅ and ABTS⋅þ scavenging assays. Cytoprotective properties of AuNPL towards HaCaT cells were examined by measuring the physiological and H2O2 induced intracellular reactive oxygen species (ROS) levels using flow cytometry. Also, genotoxicity of AuNPL in HaCaT cells was investigated by the single cell alkaline comet assay. Results: Spherical AuNPL, stable in aqueous solution up to six months at 4 ◦C were obtained in the synthesis. The selectivity in the cytotoxic action of AuNPL on HeLa and A375 cancer cells compared with their cytotoxicity on normal keratinocytes HaCaT was observed. AuNPL exerted their cytotoxic activity against HeLa cells through accumulation of the cells in the subG1 phase of the cell cycle, inducing the apoptotic cell death mediated by the activation of caspase-3 − 8, and − 9. AuNPL antioxidative potential was confirmed by DPPH⋅ and ABTS⋅þ scavenging assays. IC50 concentration of AuNPL exerted cytoprotective effect against HaCaT cells by the significant reduction of the physiological intracellular ROS level. Additionally, AuNPL were shown as more cytoprotective towards HaCaT cells then luteolin due to the more successful elimination of H2O2 induced intracellular ROS. Moreover, nontoxic concentrations of AuNPL did not cause considerable DNA damage of HaCaT cells, indicating low genotoxicity of the nanoparticles.",
journal = "Journal of Trace Elements in Medicine and Biology",
title = "Anticancer effect of novel luteolin capped gold nanoparticles selectively cytotoxic towards human cervical adenocarcinoma HeLa cells: An in vitro approach",
volume = "80",
pages = "127286",
doi = "10.1016/j.jtemb.2023.127286"
}

Matić, I. Z., Mraković, A., Rakočević, Z., Stoiljković, M., Pavlović, V. B.,& Momić, T.. (2023). Anticancer effect of novel luteolin capped gold nanoparticles selectively cytotoxic towards human cervical adenocarcinoma HeLa cells: An in vitro approach. in Journal of Trace Elements in Medicine and Biology, 80, 127286.
https://doi.org/10.1016/j.jtemb.2023.127286

Matić IZ, Mraković A, Rakočević Z, Stoiljković M, Pavlović VB, Momić T. Anticancer effect of novel luteolin capped gold nanoparticles selectively cytotoxic towards human cervical adenocarcinoma HeLa cells: An in vitro approach. in Journal of Trace Elements in Medicine and Biology. 2023;80:127286.
doi:10.1016/j.jtemb.2023.127286 .

Matić, Ivana Z., Mraković, Ana, Rakočević, Zlatko, Stoiljković, Milovan, Pavlović, Vladimir B., Momić, Tatjana, "Anticancer effect of novel luteolin capped gold nanoparticles selectively cytotoxic towards human cervical adenocarcinoma HeLa cells: An in vitro approach" in Journal of Trace Elements in Medicine and Biology, 80 (2023):127286,
https://doi.org/10.1016/j.jtemb.2023.127286 . .

TY  - JOUR
AU  - Marković, Maja D.
AU  - Tadić, Julijana D.
AU  - Savić, Sanja I.
AU  - Matić, Ivana Z.
AU  - Stanojković, Tatjana P.
AU  - Mijin, Dušan Ž.
AU  - Panić, Vesna V.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10262
AB  - Researchers are faced with everyday demands for safer and more efficient therapy for many diseases, especially serious one such as various types of cancer. Numerous anticancer drugs are poorly-water soluble and therefore their encapsulation and controlled release remain quite challenge. In present study, we deepened our research of hydrophilic carrier based on poly(methacrylic acid) and casein (PMAC) by investigating its potential for encapsulation and controlled release of novel poorly water-soluble dihydropyrimidion-azo-pyridon compound (DHPMP). DHPMP is a dye that has been proven to show cytotoxic activity against chronic myeloid leukemia K562 cells. By encapsulating DHPMP into the carrier and delivering it into the intestines, DHPMP absorption could be the fastest and the number of therapeutic doses and side effects can be reduced. Carriers based on PMAC and DHPMP (PMAC-DHPMP) were synthetized and characterized by FTIR, SEM and single compression tests. The swelling behavior of PMAC-DHPMP carriers and cumulative DHPMP release were investigated depending on the amount of crosslinker and encapsulated DHPMP in two media which were simulating pH environments in human stomach and intestines. The prolonged and controlled release of DHPMP was achieved. In vitro cytotoxic activity of PMAC-DHPMP carriers against K562 cells and the cell cycle analysis showed great potential of the carriers for application in leukemia treatment.
T2  - Journal of Biomedical Materials Research - Part A
T1  - Soft 3D hybrid network for delivery and controlled release of poorly soluble dihydropyrimidinone compound: An insight into the novel system for potential application in leukemia treatment
DO  - 10.1002/jbm.a.37396
ER  - 

@article{
author = "Marković, Maja D. and Tadić, Julijana D. and Savić, Sanja I. and Matić, Ivana Z. and Stanojković, Tatjana P. and Mijin, Dušan Ž. and Panić, Vesna V.",
year = "2022",
abstract = "Researchers are faced with everyday demands for safer and more efficient therapy for many diseases, especially serious one such as various types of cancer. Numerous anticancer drugs are poorly-water soluble and therefore their encapsulation and controlled release remain quite challenge. In present study, we deepened our research of hydrophilic carrier based on poly(methacrylic acid) and casein (PMAC) by investigating its potential for encapsulation and controlled release of novel poorly water-soluble dihydropyrimidion-azo-pyridon compound (DHPMP). DHPMP is a dye that has been proven to show cytotoxic activity against chronic myeloid leukemia K562 cells. By encapsulating DHPMP into the carrier and delivering it into the intestines, DHPMP absorption could be the fastest and the number of therapeutic doses and side effects can be reduced. Carriers based on PMAC and DHPMP (PMAC-DHPMP) were synthetized and characterized by FTIR, SEM and single compression tests. The swelling behavior of PMAC-DHPMP carriers and cumulative DHPMP release were investigated depending on the amount of crosslinker and encapsulated DHPMP in two media which were simulating pH environments in human stomach and intestines. The prolonged and controlled release of DHPMP was achieved. In vitro cytotoxic activity of PMAC-DHPMP carriers against K562 cells and the cell cycle analysis showed great potential of the carriers for application in leukemia treatment.",
journal = "Journal of Biomedical Materials Research - Part A",
title = "Soft 3D hybrid network for delivery and controlled release of poorly soluble dihydropyrimidinone compound: An insight into the novel system for potential application in leukemia treatment",
doi = "10.1002/jbm.a.37396"
}

Marković, M. D., Tadić, J. D., Savić, S. I., Matić, I. Z., Stanojković, T. P., Mijin, D. Ž.,& Panić, V. V.. (2022). Soft 3D hybrid network for delivery and controlled release of poorly soluble dihydropyrimidinone compound: An insight into the novel system for potential application in leukemia treatment. in Journal of Biomedical Materials Research - Part A.
https://doi.org/10.1002/jbm.a.37396

Marković MD, Tadić JD, Savić SI, Matić IZ, Stanojković TP, Mijin DŽ, Panić VV. Soft 3D hybrid network for delivery and controlled release of poorly soluble dihydropyrimidinone compound: An insight into the novel system for potential application in leukemia treatment. in Journal of Biomedical Materials Research - Part A. 2022;.
doi:10.1002/jbm.a.37396 .

Marković, Maja D., Tadić, Julijana D., Savić, Sanja I., Matić, Ivana Z., Stanojković, Tatjana P., Mijin, Dušan Ž., Panić, Vesna V., "Soft 3D hybrid network for delivery and controlled release of poorly soluble dihydropyrimidinone compound: An insight into the novel system for potential application in leukemia treatment" in Journal of Biomedical Materials Research - Part A (2022),
https://doi.org/10.1002/jbm.a.37396 . .

TY  - JOUR
AU  - Mališić, Emina
AU  - Petrović, Nina
AU  - Brengues, Muriel
AU  - Azria, David
AU  - Matić, Ivana Z.
AU  - Srbljak Ćuk, Ivana
AU  - Kopčalić, Katarina
AU  - Stanojković, Tatjana P.
AU  - Nikitović, Marina
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10544
AB  - The genetic background of each person might affect the severity of radiotherapy (RT)-induced normal tissue toxicity. The aim of study was to evaluate the influence of TGFB1 C-509T and Leu10Pro, XRCC1 Arg280His and XRCC3 Thr241Met polymorphisms as well as the level of radiation-induced CD8 T-lymphocyte apoptosis (RILA) on adverse effects of RT for prostate cancer (PCa). The study included 88 patients with localized or locally advanced PCa who were treated with RT. The polymorphisms were determined by PCR–RFLP analysis on DNA from peripheral blood mononuclear cells. RILA values were measured by flow cytometry. We found that CT genotype of TGFB1 C-509T could be protective biomarker for acute genitourinary (GU) and gastrointestinal (GI) radiotoxicity, while Thr variant of XRCC3 Thr241Met could predict the risk for acute GU radiotoxicity. Correlation between RILA values and toxicity was not detected. Univariate logistic regression analysis showed that Gleason score and risk group were risk factors for late GU, while for late GI radiotoxicity it was diabetes mellitus type 2. However, in multivariate model those were not proven to be significant and independent risk factors. Identification of assays combination predicting individual radiosensitivity is a crucial step towards personalized RT approach.
T2  - Scientific Reports
T1  - Association of polymorphisms in TGFB1, XRCC1, XRCC3 genes and CD8 T-lymphocyte apoptosis with adverse effect of radiotherapy for prostate cancer
VL  - 12
IS  - 1
SP  - 21306
DO  - 10.1038/s41598-022-25328-6
ER  - 

@article{
author = "Mališić, Emina and Petrović, Nina and Brengues, Muriel and Azria, David and Matić, Ivana Z. and Srbljak Ćuk, Ivana and Kopčalić, Katarina and Stanojković, Tatjana P. and Nikitović, Marina",
year = "2022",
abstract = "The genetic background of each person might affect the severity of radiotherapy (RT)-induced normal tissue toxicity. The aim of study was to evaluate the influence of TGFB1 C-509T and Leu10Pro, XRCC1 Arg280His and XRCC3 Thr241Met polymorphisms as well as the level of radiation-induced CD8 T-lymphocyte apoptosis (RILA) on adverse effects of RT for prostate cancer (PCa). The study included 88 patients with localized or locally advanced PCa who were treated with RT. The polymorphisms were determined by PCR–RFLP analysis on DNA from peripheral blood mononuclear cells. RILA values were measured by flow cytometry. We found that CT genotype of TGFB1 C-509T could be protective biomarker for acute genitourinary (GU) and gastrointestinal (GI) radiotoxicity, while Thr variant of XRCC3 Thr241Met could predict the risk for acute GU radiotoxicity. Correlation between RILA values and toxicity was not detected. Univariate logistic regression analysis showed that Gleason score and risk group were risk factors for late GU, while for late GI radiotoxicity it was diabetes mellitus type 2. However, in multivariate model those were not proven to be significant and independent risk factors. Identification of assays combination predicting individual radiosensitivity is a crucial step towards personalized RT approach.",
journal = "Scientific Reports",
title = "Association of polymorphisms in TGFB1, XRCC1, XRCC3 genes and CD8 T-lymphocyte apoptosis with adverse effect of radiotherapy for prostate cancer",
volume = "12",
number = "1",
pages = "21306",
doi = "10.1038/s41598-022-25328-6"
}

Mališić, E., Petrović, N., Brengues, M., Azria, D., Matić, I. Z., Srbljak Ćuk, I., Kopčalić, K., Stanojković, T. P.,& Nikitović, M.. (2022). Association of polymorphisms in TGFB1, XRCC1, XRCC3 genes and CD8 T-lymphocyte apoptosis with adverse effect of radiotherapy for prostate cancer. in Scientific Reports, 12(1), 21306.
https://doi.org/10.1038/s41598-022-25328-6

Mališić E, Petrović N, Brengues M, Azria D, Matić IZ, Srbljak Ćuk I, Kopčalić K, Stanojković TP, Nikitović M. Association of polymorphisms in TGFB1, XRCC1, XRCC3 genes and CD8 T-lymphocyte apoptosis with adverse effect of radiotherapy for prostate cancer. in Scientific Reports. 2022;12(1):21306.
doi:10.1038/s41598-022-25328-6 .

Mališić, Emina, Petrović, Nina, Brengues, Muriel, Azria, David, Matić, Ivana Z., Srbljak Ćuk, Ivana, Kopčalić, Katarina, Stanojković, Tatjana P., Nikitović, Marina, "Association of polymorphisms in TGFB1, XRCC1, XRCC3 genes and CD8 T-lymphocyte apoptosis with adverse effect of radiotherapy for prostate cancer" in Scientific Reports, 12, no. 1 (2022):21306,
https://doi.org/10.1038/s41598-022-25328-6 . .

TY  - JOUR
AU  - Petrović, Nina
AU  - Ergün, Sercan
AU  - Đorđić-Crnogorac, Marija
AU  - Stanojković, Tatjana
AU  - Mališić, Emina
AU  - Matić Ivana Z.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10706
AB  - Phytochemicals and bioactive substances derived from a wide range of plant extracts have been reported to exert various anticancer effects. Prostate cancer is one of the leading causes of cancer-related deaths within the male population. Prostate cancer-specific miRNA signatures were associated with cancer formation and progression, with various subtypes, and response to therapy. MicroRNA levels of expression were shown to change after the treatment of various compounds and substances extracted from natural products. Natural herbal compounds were shown to induce variations in miRNA expression levels in cancer cells. The aims of this study were to investigate the cytotoxic effects of methanol, ethyl-acetate, and hexane extracts obtained from branch-body part and flowers of Hypericum perforatum L. against humane PC-3 and DU 145 and to test potential miRNA-128/133b/155/193a/206/21/335 signature changes and differences between the two prostate cancer cell lines. Cytotoxic activity of H. perforatum extracts, their effects on cell cycle distribution, and miRNA expression levels were examined in humane PC-3 and DU 145 prostate cancer cells by MTT cell survival assay, flow cytometry, and quantitative real-time PCR. Hexane extract of flowers showed the strongest intensity of cytotoxic activity against PC-3 and DU 145 cells. The highest increase in the percentage of PC-3 cells in the subG1 phase was observed in cell samples treated with hexane extract of flowers and branch-body part. Significant differences in miRNA-128/133b/155/193a/206/21/335 levels were observed between PC-3 and DU 145 cell lines, especially in samples treated with flower extracts compared with the branch-body part. Conclusions: Investigated extracts have significant anticancer potential not only from the aspects of cytotoxicity and cell cycle effects but also from the aspect of lowering oncogenic or increasing tumor-suppressive miRNAs. The best effect might be the increase of tumor-suppressive miR-128 (accompanied by miR-193a) induced by the hexane extract of the flowers, which also exerted the highest cytotoxic activity. Hexane extract of flowers may be the candidate for further investigation for improving the efficiency of standard therapies for PCa. A miRNA signature might be cell-type specific after the treatment with H. perforatum extracts.
T2  - Genetika
T1  - Hypericum perforatum L. extracts exert cytotoxic effects and show different miRNA signatures in PC-3 and DU 145 prostate cancer cells
VL  - 54
IS  - 3
SP  - 1249
EP  - 1270
DO  - 10.2298/GENSR2203249P
ER  - 

@article{
author = "Petrović, Nina and Ergün, Sercan and Đorđić-Crnogorac, Marija and Stanojković, Tatjana and Mališić, Emina and Matić Ivana Z.",
year = "2022",
abstract = "Phytochemicals and bioactive substances derived from a wide range of plant extracts have been reported to exert various anticancer effects. Prostate cancer is one of the leading causes of cancer-related deaths within the male population. Prostate cancer-specific miRNA signatures were associated with cancer formation and progression, with various subtypes, and response to therapy. MicroRNA levels of expression were shown to change after the treatment of various compounds and substances extracted from natural products. Natural herbal compounds were shown to induce variations in miRNA expression levels in cancer cells. The aims of this study were to investigate the cytotoxic effects of methanol, ethyl-acetate, and hexane extracts obtained from branch-body part and flowers of Hypericum perforatum L. against humane PC-3 and DU 145 and to test potential miRNA-128/133b/155/193a/206/21/335 signature changes and differences between the two prostate cancer cell lines. Cytotoxic activity of H. perforatum extracts, their effects on cell cycle distribution, and miRNA expression levels were examined in humane PC-3 and DU 145 prostate cancer cells by MTT cell survival assay, flow cytometry, and quantitative real-time PCR. Hexane extract of flowers showed the strongest intensity of cytotoxic activity against PC-3 and DU 145 cells. The highest increase in the percentage of PC-3 cells in the subG1 phase was observed in cell samples treated with hexane extract of flowers and branch-body part. Significant differences in miRNA-128/133b/155/193a/206/21/335 levels were observed between PC-3 and DU 145 cell lines, especially in samples treated with flower extracts compared with the branch-body part. Conclusions: Investigated extracts have significant anticancer potential not only from the aspects of cytotoxicity and cell cycle effects but also from the aspect of lowering oncogenic or increasing tumor-suppressive miRNAs. The best effect might be the increase of tumor-suppressive miR-128 (accompanied by miR-193a) induced by the hexane extract of the flowers, which also exerted the highest cytotoxic activity. Hexane extract of flowers may be the candidate for further investigation for improving the efficiency of standard therapies for PCa. A miRNA signature might be cell-type specific after the treatment with H. perforatum extracts.",
journal = "Genetika",
title = "Hypericum perforatum L. extracts exert cytotoxic effects and show different miRNA signatures in PC-3 and DU 145 prostate cancer cells",
volume = "54",
number = "3",
pages = "1249-1270",
doi = "10.2298/GENSR2203249P"
}

Petrović, N., Ergün, S., Đorđić-Crnogorac, M., Stanojković, T., Mališić, E.,& Matić Ivana Z.. (2022). Hypericum perforatum L. extracts exert cytotoxic effects and show different miRNA signatures in PC-3 and DU 145 prostate cancer cells. in Genetika, 54(3), 1249-1270.
https://doi.org/10.2298/GENSR2203249P

Petrović N, Ergün S, Đorđić-Crnogorac M, Stanojković T, Mališić E, Matić Ivana Z.. Hypericum perforatum L. extracts exert cytotoxic effects and show different miRNA signatures in PC-3 and DU 145 prostate cancer cells. in Genetika. 2022;54(3):1249-1270.
doi:10.2298/GENSR2203249P .

Petrović, Nina, Ergün, Sercan, Đorđić-Crnogorac, Marija, Stanojković, Tatjana, Mališić, Emina, Matić Ivana Z., "Hypericum perforatum L. extracts exert cytotoxic effects and show different miRNA signatures in PC-3 and DU 145 prostate cancer cells" in Genetika, 54, no. 3 (2022):1249-1270,
https://doi.org/10.2298/GENSR2203249P . .

TY  - CONF
AU  - Pašić, Ivana
AU  - Srbljak Ćuk, Ivana
AU  - Petrović, Nina
AU  - Matić, Ivana
AU  - Džudžević-Čančar, Hurija
AU  - Dedić, Alema
AU  - Alispahić, Amra
AU  - Boškailo, Emina
AU  - Stanojković, Tatjana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12300
AB  - Lavandula angustifolia Mill. (lavender) is an aromatic and medicinal herb whose flower essential oils (EO) are widely used for the treatment of gastrointestinal, nervous, and rheumatic disorders, and in the perfume industry. Laurus nobilis L. (laurel bay) is an evergreen tree whose EOs have antimicrobial and anti-inflammatory effects. Lavender and bay were collected from Sarajevo and Mostar in Bosnia and Herzegovina. The extraction was performed by hydrodistillation in Clevenger-type apparatus. Phytochemical composition was analyzed by gas chromatography coupled with mass spectrometry. Cytotoxic activities of lavender EO and bay leaf, fruit and seed EOs were investigated against human cervical adenocarcinoma HeLa cells and non-transformed human lung fibroblasts MRC-5 by MTT cell survival assay. Cell cycle phase distribution was examined by flow cytometry. In bay EOs the most abundant component was 1,8-cineole, followed by linalool, bicyclic monoterpenes sabinene, αpinene, and β-pinene. Components identified in the fruit and seed, but not in the leaf were (E)-β-ocymene, camphene, β-elemene, bornyl acetate and trans-caryophyllene. The major component of lavender extract was linalool accompanied by linalyl acetate, lavandulyl acetate, camphor, 1,8-cneole, borneol, α-terpineol, and terpinene-4-ol. The four tested EOs showed concentration-dependent cytotoxic effects on HeLa and MRC-5 cells. Among examined EOs, lavender EO exerted the strongest cytotoxic activity on HeLa cells with IC50 value of 0.11 µL/mL. Bay seed and fruit EOs exerted stronger cytotoxicity on HeLa cells than bay leaf EO (IC50 values: 0.17, 0.21, and 3.35 µL/mL, respectively). When compared with sensitivity of HeLa cells, normal MRC-5 cells showed similar sensitivity to the cytotoxic activity of the four tested EOs. Lavender EO applied at IC50 concentration, during 24 h caused remarkable increase in the percentage of HeLa cells within the subG1 cell cycle phase, in comparison with control cells (64.69% vs 2.47%). Pretreatment with caspase-3, caspase-8 or caspase-9 inhibitor before 24 h treatment with lavender EO did not cause changes in the percentage of cells in the subG1 phase in comparison with HeLa cells exposed only to lavender oil. Our results showed that lavender and bay EOs exerted potent cytotoxic activity against HeLa cells. Additional investigations are necessary to explore cytotoxic effects of these EOs against various cancer cell lines and mechanisms underlying anticancer effects.
PB  - Zagreb : Croatian Association for Cancer Research
C3  - 6th Meeting of the Croatian Association for Cancer Research “HDIR-6: Targeting Cancer” : Book of abstracts
T1  - Cytotoxic Effects of Lavandula angustifolia Mill. and Laurus nobilis L. Essential Oils on Human Cervical Adenocarcinoma Cells
SP  - 43
EP  - 43
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12300
ER  - 

@conference{
author = "Pašić, Ivana and Srbljak Ćuk, Ivana and Petrović, Nina and Matić, Ivana and Džudžević-Čančar, Hurija and Dedić, Alema and Alispahić, Amra and Boškailo, Emina and Stanojković, Tatjana",
year = "2022",
abstract = "Lavandula angustifolia Mill. (lavender) is an aromatic and medicinal herb whose flower essential oils (EO) are widely used for the treatment of gastrointestinal, nervous, and rheumatic disorders, and in the perfume industry. Laurus nobilis L. (laurel bay) is an evergreen tree whose EOs have antimicrobial and anti-inflammatory effects. Lavender and bay were collected from Sarajevo and Mostar in Bosnia and Herzegovina. The extraction was performed by hydrodistillation in Clevenger-type apparatus. Phytochemical composition was analyzed by gas chromatography coupled with mass spectrometry. Cytotoxic activities of lavender EO and bay leaf, fruit and seed EOs were investigated against human cervical adenocarcinoma HeLa cells and non-transformed human lung fibroblasts MRC-5 by MTT cell survival assay. Cell cycle phase distribution was examined by flow cytometry. In bay EOs the most abundant component was 1,8-cineole, followed by linalool, bicyclic monoterpenes sabinene, αpinene, and β-pinene. Components identified in the fruit and seed, but not in the leaf were (E)-β-ocymene, camphene, β-elemene, bornyl acetate and trans-caryophyllene. The major component of lavender extract was linalool accompanied by linalyl acetate, lavandulyl acetate, camphor, 1,8-cneole, borneol, α-terpineol, and terpinene-4-ol. The four tested EOs showed concentration-dependent cytotoxic effects on HeLa and MRC-5 cells. Among examined EOs, lavender EO exerted the strongest cytotoxic activity on HeLa cells with IC50 value of 0.11 µL/mL. Bay seed and fruit EOs exerted stronger cytotoxicity on HeLa cells than bay leaf EO (IC50 values: 0.17, 0.21, and 3.35 µL/mL, respectively). When compared with sensitivity of HeLa cells, normal MRC-5 cells showed similar sensitivity to the cytotoxic activity of the four tested EOs. Lavender EO applied at IC50 concentration, during 24 h caused remarkable increase in the percentage of HeLa cells within the subG1 cell cycle phase, in comparison with control cells (64.69% vs 2.47%). Pretreatment with caspase-3, caspase-8 or caspase-9 inhibitor before 24 h treatment with lavender EO did not cause changes in the percentage of cells in the subG1 phase in comparison with HeLa cells exposed only to lavender oil. Our results showed that lavender and bay EOs exerted potent cytotoxic activity against HeLa cells. Additional investigations are necessary to explore cytotoxic effects of these EOs against various cancer cell lines and mechanisms underlying anticancer effects.",
publisher = "Zagreb : Croatian Association for Cancer Research",
journal = "6th Meeting of the Croatian Association for Cancer Research “HDIR-6: Targeting Cancer” : Book of abstracts",
title = "Cytotoxic Effects of Lavandula angustifolia Mill. and Laurus nobilis L. Essential Oils on Human Cervical Adenocarcinoma Cells",
pages = "43-43",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12300"
}

Pašić, I., Srbljak Ćuk, I., Petrović, N., Matić, I., Džudžević-Čančar, H., Dedić, A., Alispahić, A., Boškailo, E.,& Stanojković, T.. (2022). Cytotoxic Effects of Lavandula angustifolia Mill. and Laurus nobilis L. Essential Oils on Human Cervical Adenocarcinoma Cells. in 6th Meeting of the Croatian Association for Cancer Research “HDIR-6: Targeting Cancer” : Book of abstracts
Zagreb : Croatian Association for Cancer Research., 43-43.
https://hdl.handle.net/21.15107/rcub_vinar_12300

Pašić I, Srbljak Ćuk I, Petrović N, Matić I, Džudžević-Čančar H, Dedić A, Alispahić A, Boškailo E, Stanojković T. Cytotoxic Effects of Lavandula angustifolia Mill. and Laurus nobilis L. Essential Oils on Human Cervical Adenocarcinoma Cells. in 6th Meeting of the Croatian Association for Cancer Research “HDIR-6: Targeting Cancer” : Book of abstracts. 2022;:43-43.
https://hdl.handle.net/21.15107/rcub_vinar_12300 .

Pašić, Ivana, Srbljak Ćuk, Ivana, Petrović, Nina, Matić, Ivana, Džudžević-Čančar, Hurija, Dedić, Alema, Alispahić, Amra, Boškailo, Emina, Stanojković, Tatjana, "Cytotoxic Effects of Lavandula angustifolia Mill. and Laurus nobilis L. Essential Oils on Human Cervical Adenocarcinoma Cells" in 6th Meeting of the Croatian Association for Cancer Research “HDIR-6: Targeting Cancer” : Book of abstracts (2022):43-43,
https://hdl.handle.net/21.15107/rcub_vinar_12300 .

TY  - JOUR
AU  - Tadić, Julijana D.
AU  - Lađarević, Jelena M.
AU  - Vitnik, Željko J.
AU  - Vitnik, Vesna D.
AU  - Stanojković, Tatjana P.
AU  - Matić, Ivana Z.
AU  - Mijin, Dušan Ž.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10890
AB  - Seven novel azo dyes with 2-pyridone and dihydropyrimidinone moieties have been synthesized and thoroughly characterized. The azo-hydrazone tautomerism has been investigated by experimental and theoretical approaches. The optimizations of geometries have been performed with density functional theory (DFT). The vibrational and NMR spectra were calculated and correlated with experimental ones. Furthermore, quantum chemical descriptors were calculated and MEP maps were plotted to determine biological reactivity of dyes. The antioxidant assay evinced that 5, 6 and 7 are promising antioxidant candidates. In vitro cytotoxic activity was studied against three malignant cell lines: prostate adenocarcinoma (PC-3), lung carcinoma (A549) and chronic myelogenous leukemia (K562), as well as against human normal lung fibroblasts (MRC-5), using MTT assay. Examination of cytotoxic effects on human cancer cell lines showed the concentration dependent cytotoxicity of all investigated compounds. The K562 cells were the most sensitive to the cytotoxicity of the compounds 3, 5 and 6, wherein compound 5 was particularly prominent and selective in cytotoxic action between K562 (24.97 μM) and PC-3 (48.98 μM) cancer cells, and normal MRC-5 (91.11 μM) cells. Moreover, the cell cycle analysis of compound 5 was examined in K562 cells, by flow cytometry, to study its mechanism of anticancer action. Finally, in silico evaluation of physicochemical parameters, druglikeness and ADME properties showed that investigated compounds are orally bioavailable with no permeation to the blood brain barrier.
T2  - Dyes and Pigments
T1  - Novel azo pyridone dyes based on dihydropyrimidinone skeleton: Synthesis, DFT study and anticancer activity
VL  - 187
SP  - 109123
DO  - 10.1016/j.dyepig.2020.109123
ER  - 

@article{
author = "Tadić, Julijana D. and Lađarević, Jelena M. and Vitnik, Željko J. and Vitnik, Vesna D. and Stanojković, Tatjana P. and Matić, Ivana Z. and Mijin, Dušan Ž.",
year = "2021",
abstract = "Seven novel azo dyes with 2-pyridone and dihydropyrimidinone moieties have been synthesized and thoroughly characterized. The azo-hydrazone tautomerism has been investigated by experimental and theoretical approaches. The optimizations of geometries have been performed with density functional theory (DFT). The vibrational and NMR spectra were calculated and correlated with experimental ones. Furthermore, quantum chemical descriptors were calculated and MEP maps were plotted to determine biological reactivity of dyes. The antioxidant assay evinced that 5, 6 and 7 are promising antioxidant candidates. In vitro cytotoxic activity was studied against three malignant cell lines: prostate adenocarcinoma (PC-3), lung carcinoma (A549) and chronic myelogenous leukemia (K562), as well as against human normal lung fibroblasts (MRC-5), using MTT assay. Examination of cytotoxic effects on human cancer cell lines showed the concentration dependent cytotoxicity of all investigated compounds. The K562 cells were the most sensitive to the cytotoxicity of the compounds 3, 5 and 6, wherein compound 5 was particularly prominent and selective in cytotoxic action between K562 (24.97 μM) and PC-3 (48.98 μM) cancer cells, and normal MRC-5 (91.11 μM) cells. Moreover, the cell cycle analysis of compound 5 was examined in K562 cells, by flow cytometry, to study its mechanism of anticancer action. Finally, in silico evaluation of physicochemical parameters, druglikeness and ADME properties showed that investigated compounds are orally bioavailable with no permeation to the blood brain barrier.",
journal = "Dyes and Pigments",
title = "Novel azo pyridone dyes based on dihydropyrimidinone skeleton: Synthesis, DFT study and anticancer activity",
volume = "187",
pages = "109123",
doi = "10.1016/j.dyepig.2020.109123"
}

Tadić, J. D., Lađarević, J. M., Vitnik, Ž. J., Vitnik, V. D., Stanojković, T. P., Matić, I. Z.,& Mijin, D. Ž.. (2021). Novel azo pyridone dyes based on dihydropyrimidinone skeleton: Synthesis, DFT study and anticancer activity. in Dyes and Pigments, 187, 109123.
https://doi.org/10.1016/j.dyepig.2020.109123

Tadić JD, Lađarević JM, Vitnik ŽJ, Vitnik VD, Stanojković TP, Matić IZ, Mijin DŽ. Novel azo pyridone dyes based on dihydropyrimidinone skeleton: Synthesis, DFT study and anticancer activity. in Dyes and Pigments. 2021;187:109123.
doi:10.1016/j.dyepig.2020.109123 .

Tadić, Julijana D., Lađarević, Jelena M., Vitnik, Željko J., Vitnik, Vesna D., Stanojković, Tatjana P., Matić, Ivana Z., Mijin, Dušan Ž., "Novel azo pyridone dyes based on dihydropyrimidinone skeleton: Synthesis, DFT study and anticancer activity" in Dyes and Pigments, 187 (2021):109123,
https://doi.org/10.1016/j.dyepig.2020.109123 . .

TY  - JOUR
AU  - Matić, Ivana Z.
AU  - Ergün, Sercan
AU  - Đorđić Crnogorac, Marija
AU  - Misir, Sema
AU  - Aliyazicioğlu, Yüksel
AU  - Damjanović, Ana
AU  - Džudžević-Čančar, Hurija
AU  - Stanojković, Tatjana P.
AU  - Konanç, Kalbiye
AU  - Petrović, Nina
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9406
AB  - Six extracts were obtained from plant species Hypericum perforatum L., collected at Samsun in Turkey. The aim of this study was to examine the mechanisms of the anticancer activity of these extracts. Methanol, ethyl-acetate and hexane were used as a solvents for extraction from both branch-body part of the plant (extracts 1, 2 and 3) and from plant flowers (extracts 4, 5 and 6). The cytotoxic effects of the extracts were determined against 2D and 3D cancer cell models. Cell cycle changes of treated HeLa cells were analyzed by flow cytometry. Measurements of gene and microRNA expression levels in treated HeLa cells were done by quantitative real time PCR. Five examined extracts (2–6) exerted selective concentration-dependent cytotoxic effects on HeLa, K562, and A549 cancer cells, while the extract 1 exhibited very weak cytotoxicity. The extract 6 showed the highest intensity of cytotoxic activity. All tested extracts (2–6) demonstrated the ability to induce apoptosis in HeLa cells through activation of caspase-3. These extracts remarkably decreased gene expression levels of MMP2, MMP9, TIMP3, and VEGFA in HeLa cells. Flower extracts might have stronger effects on miR128/193a-5p/335 level changes than branch-body extracts. Hypericum perforatum extracts exerted weaker cytotoxic effects on 3D HeLa spheroids when compared with their effects on 2D monolayer HeLa cells. Taken together, results of our research may suggest the promising anticancer properties of the Hypericum perforatum extracts. © 2021, The Author(s), under exclusive licence to Springer Nature B.V.
T2  - Cytotechnology
T1  - Cytotoxic activities of Hypericum perforatum L. extracts against 2D and 3D cancer cell models
VL  - 73
IS  - 3
SP  - 373
EP  - 389
DO  - 10.1007/s10616-021-00464-5
ER  - 

@article{
author = "Matić, Ivana Z. and Ergün, Sercan and Đorđić Crnogorac, Marija and Misir, Sema and Aliyazicioğlu, Yüksel and Damjanović, Ana and Džudžević-Čančar, Hurija and Stanojković, Tatjana P. and Konanç, Kalbiye and Petrović, Nina",
year = "2021",
abstract = "Six extracts were obtained from plant species Hypericum perforatum L., collected at Samsun in Turkey. The aim of this study was to examine the mechanisms of the anticancer activity of these extracts. Methanol, ethyl-acetate and hexane were used as a solvents for extraction from both branch-body part of the plant (extracts 1, 2 and 3) and from plant flowers (extracts 4, 5 and 6). The cytotoxic effects of the extracts were determined against 2D and 3D cancer cell models. Cell cycle changes of treated HeLa cells were analyzed by flow cytometry. Measurements of gene and microRNA expression levels in treated HeLa cells were done by quantitative real time PCR. Five examined extracts (2–6) exerted selective concentration-dependent cytotoxic effects on HeLa, K562, and A549 cancer cells, while the extract 1 exhibited very weak cytotoxicity. The extract 6 showed the highest intensity of cytotoxic activity. All tested extracts (2–6) demonstrated the ability to induce apoptosis in HeLa cells through activation of caspase-3. These extracts remarkably decreased gene expression levels of MMP2, MMP9, TIMP3, and VEGFA in HeLa cells. Flower extracts might have stronger effects on miR128/193a-5p/335 level changes than branch-body extracts. Hypericum perforatum extracts exerted weaker cytotoxic effects on 3D HeLa spheroids when compared with their effects on 2D monolayer HeLa cells. Taken together, results of our research may suggest the promising anticancer properties of the Hypericum perforatum extracts. © 2021, The Author(s), under exclusive licence to Springer Nature B.V.",
journal = "Cytotechnology",
title = "Cytotoxic activities of Hypericum perforatum L. extracts against 2D and 3D cancer cell models",
volume = "73",
number = "3",
pages = "373-389",
doi = "10.1007/s10616-021-00464-5"
}

Matić, I. Z., Ergün, S., Đorđić Crnogorac, M., Misir, S., Aliyazicioğlu, Y., Damjanović, A., Džudžević-Čančar, H., Stanojković, T. P., Konanç, K.,& Petrović, N.. (2021). Cytotoxic activities of Hypericum perforatum L. extracts against 2D and 3D cancer cell models. in Cytotechnology, 73(3), 373-389.
https://doi.org/10.1007/s10616-021-00464-5

Matić IZ, Ergün S, Đorđić Crnogorac M, Misir S, Aliyazicioğlu Y, Damjanović A, Džudžević-Čančar H, Stanojković TP, Konanç K, Petrović N. Cytotoxic activities of Hypericum perforatum L. extracts against 2D and 3D cancer cell models. in Cytotechnology. 2021;73(3):373-389.
doi:10.1007/s10616-021-00464-5 .

Matić, Ivana Z., Ergün, Sercan, Đorđić Crnogorac, Marija, Misir, Sema, Aliyazicioğlu, Yüksel, Damjanović, Ana, Džudžević-Čančar, Hurija, Stanojković, Tatjana P., Konanç, Kalbiye, Petrović, Nina, "Cytotoxic activities of Hypericum perforatum L. extracts against 2D and 3D cancer cell models" in Cytotechnology, 73, no. 3 (2021):373-389,
https://doi.org/10.1007/s10616-021-00464-5 . .

TY  - JOUR
AU  - Stanojković, Tatjana P.
AU  - Matić, Ivana Z.
AU  - Petrović, Nina
AU  - Stanković, Vesna
AU  - Kopčalić, Katarina
AU  - Besu, Irina
AU  - Đorđić Crnogorac, Marija
AU  - Mališić, Emina
AU  - Mirjačić-Martinović, Katarina
AU  - Vuletić, Ana
AU  - Bukumirić, Zoran
AU  - Žižak, Željko
AU  - Veldwijk, Marlon
AU  - Herskind, Carsten
AU  - Nikitović, Marina
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9713
AB  - One of the challenges of radiation oncology in the era of personalized medicine is identification of biomarkers associated with individual radiosensitivity. The aim of research was to evaluate the possible clinical value of the associations between clinical, physical, and biological factors, and risk for development of acute radiotoxicity in patients with prostate cancer. The study involved forty four patients treated with three-dimensional conformal radiotherapy. The concentrations of IL-1β, IL-2, IL-6, IFN-γ and TGF-β1 were assessed before radiotherapy, after 5th, 15th and 25th radiotherapy fractions, at the end, and 1 month after the end of radiotherapy. Cytokine gene expression was determined in peripheral blood mononuclear cells. The univariate analysis of circulating cytokine levels during radiotherapy showed that increased serum concentrations of IL-6 were significantly associated with higher grade of acute genitourinary toxicity. The multivariate analysis demonstrated that increased level of IL-6 during the radiotherapy was significantly associated with higher grade of acute genitourinary toxicity across treatment. TGF-β expression levels significantly decreased during course of radiotherapy. Research indicates that changes in circulating cytokine levels might be important parameter of radiotoxicity in patients with prostate cancer. These findings suggest that future studies based on multi-parameter examination are necessary for prediction of individual radiosensitivity.
T2  - Scientific Reports
T1  - Evaluation of cytokine expression and circulating immune cell subsets as potential parameters of acute radiation toxicity in prostate cancer patients
VL  - 10
IS  - 1
SP  - 19002
DO  - 10.1038/s41598-020-75812-0
ER  - 

@article{
author = "Stanojković, Tatjana P. and Matić, Ivana Z. and Petrović, Nina and Stanković, Vesna and Kopčalić, Katarina and Besu, Irina and Đorđić Crnogorac, Marija and Mališić, Emina and Mirjačić-Martinović, Katarina and Vuletić, Ana and Bukumirić, Zoran and Žižak, Željko and Veldwijk, Marlon and Herskind, Carsten and Nikitović, Marina",
year = "2020",
abstract = "One of the challenges of radiation oncology in the era of personalized medicine is identification of biomarkers associated with individual radiosensitivity. The aim of research was to evaluate the possible clinical value of the associations between clinical, physical, and biological factors, and risk for development of acute radiotoxicity in patients with prostate cancer. The study involved forty four patients treated with three-dimensional conformal radiotherapy. The concentrations of IL-1β, IL-2, IL-6, IFN-γ and TGF-β1 were assessed before radiotherapy, after 5th, 15th and 25th radiotherapy fractions, at the end, and 1 month after the end of radiotherapy. Cytokine gene expression was determined in peripheral blood mononuclear cells. The univariate analysis of circulating cytokine levels during radiotherapy showed that increased serum concentrations of IL-6 were significantly associated with higher grade of acute genitourinary toxicity. The multivariate analysis demonstrated that increased level of IL-6 during the radiotherapy was significantly associated with higher grade of acute genitourinary toxicity across treatment. TGF-β expression levels significantly decreased during course of radiotherapy. Research indicates that changes in circulating cytokine levels might be important parameter of radiotoxicity in patients with prostate cancer. These findings suggest that future studies based on multi-parameter examination are necessary for prediction of individual radiosensitivity.",
journal = "Scientific Reports",
title = "Evaluation of cytokine expression and circulating immune cell subsets as potential parameters of acute radiation toxicity in prostate cancer patients",
volume = "10",
number = "1",
pages = "19002",
doi = "10.1038/s41598-020-75812-0"
}

Stanojković, T. P., Matić, I. Z., Petrović, N., Stanković, V., Kopčalić, K., Besu, I., Đorđić Crnogorac, M., Mališić, E., Mirjačić-Martinović, K., Vuletić, A., Bukumirić, Z., Žižak, Ž., Veldwijk, M., Herskind, C.,& Nikitović, M.. (2020). Evaluation of cytokine expression and circulating immune cell subsets as potential parameters of acute radiation toxicity in prostate cancer patients. in Scientific Reports, 10(1), 19002.
https://doi.org/10.1038/s41598-020-75812-0

Stanojković TP, Matić IZ, Petrović N, Stanković V, Kopčalić K, Besu I, Đorđić Crnogorac M, Mališić E, Mirjačić-Martinović K, Vuletić A, Bukumirić Z, Žižak Ž, Veldwijk M, Herskind C, Nikitović M. Evaluation of cytokine expression and circulating immune cell subsets as potential parameters of acute radiation toxicity in prostate cancer patients. in Scientific Reports. 2020;10(1):19002.
doi:10.1038/s41598-020-75812-0 .

Stanojković, Tatjana P., Matić, Ivana Z., Petrović, Nina, Stanković, Vesna, Kopčalić, Katarina, Besu, Irina, Đorđić Crnogorac, Marija, Mališić, Emina, Mirjačić-Martinović, Katarina, Vuletić, Ana, Bukumirić, Zoran, Žižak, Željko, Veldwijk, Marlon, Herskind, Carsten, Nikitović, Marina, "Evaluation of cytokine expression and circulating immune cell subsets as potential parameters of acute radiation toxicity in prostate cancer patients" in Scientific Reports, 10, no. 1 (2020):19002,
https://doi.org/10.1038/s41598-020-75812-0 . .

TY  - JOUR
AU  - Janković, Nenad Ž.
AU  - Trifunović Ristovski, Jovana
AU  - Vraneš, Milan
AU  - Tot, Aleksandar
AU  - Petronijević, Jelena
AU  - Joksimović, Nenad
AU  - Stanojković, Tatjana P.
AU  - Đorđić Crnogorac, Marija
AU  - Petrović, Nina
AU  - Boljević, Ivana
AU  - Matić, Ivana Z.
AU  - Bogdanović, Goran A.
AU  - Mikov, Momir
AU  - Bugarčić, Zorica M.
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0045206818312598
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8071
AB  - In order to investigate potential therapeutically agents, novel products of Biginelli reaction (4a-l) were synthesized and exposed to cytotoxic and caspase activities, angiogenesis, cell cycle distribution, gene and microRNA expression levels, lipophilicity assessment and docking study. Among the twelve novel compounds (4a-l) evaluated for the cytotoxic activity, five of them (4c, 4d, 4f, 4k and 4l) that showed excellent activity on the tested cell lines (HeLa, LS174 and A549) were selected for further evaluation. Interestingly, compound 4f has up to three times higher selectivity index (SI) towards cancer cells than cisplatin (on HeLa, LS174 and A549 SI = 18.2, 13.5 and 11.2, respectively). The obtained results from cell cycle distribution and caspase activity indicate that tested compounds (4c, 4d, 4f, 4k and 4l) promoted caspase-9 activation, implicated in the intrinsic pathway of apoptosis. Lipophilicity of 4a-l was determinate by using reversed-phase high-performance liquid chromatography. © 2019 Elsevier Inc.
T2  - Bioorganic Chemistry
T1  - Discovery of the Biginelli hybrids as novel caspase-9 activators in apoptotic machines: Lipophilicity, molecular docking study, influence on angiogenesis gene and miR-21 expression levels
VL  - 86
SP  - 569
EP  - 582
DO  - 10.1016/j.bioorg.2019.02.026
ER  - 

@article{
author = "Janković, Nenad Ž. and Trifunović Ristovski, Jovana and Vraneš, Milan and Tot, Aleksandar and Petronijević, Jelena and Joksimović, Nenad and Stanojković, Tatjana P. and Đorđić Crnogorac, Marija and Petrović, Nina and Boljević, Ivana and Matić, Ivana Z. and Bogdanović, Goran A. and Mikov, Momir and Bugarčić, Zorica M.",
year = "2019",
abstract = "In order to investigate potential therapeutically agents, novel products of Biginelli reaction (4a-l) were synthesized and exposed to cytotoxic and caspase activities, angiogenesis, cell cycle distribution, gene and microRNA expression levels, lipophilicity assessment and docking study. Among the twelve novel compounds (4a-l) evaluated for the cytotoxic activity, five of them (4c, 4d, 4f, 4k and 4l) that showed excellent activity on the tested cell lines (HeLa, LS174 and A549) were selected for further evaluation. Interestingly, compound 4f has up to three times higher selectivity index (SI) towards cancer cells than cisplatin (on HeLa, LS174 and A549 SI = 18.2, 13.5 and 11.2, respectively). The obtained results from cell cycle distribution and caspase activity indicate that tested compounds (4c, 4d, 4f, 4k and 4l) promoted caspase-9 activation, implicated in the intrinsic pathway of apoptosis. Lipophilicity of 4a-l was determinate by using reversed-phase high-performance liquid chromatography. © 2019 Elsevier Inc.",
journal = "Bioorganic Chemistry",
title = "Discovery of the Biginelli hybrids as novel caspase-9 activators in apoptotic machines: Lipophilicity, molecular docking study, influence on angiogenesis gene and miR-21 expression levels",
volume = "86",
pages = "569-582",
doi = "10.1016/j.bioorg.2019.02.026"
}

Janković, N. Ž., Trifunović Ristovski, J., Vraneš, M., Tot, A., Petronijević, J., Joksimović, N., Stanojković, T. P., Đorđić Crnogorac, M., Petrović, N., Boljević, I., Matić, I. Z., Bogdanović, G. A., Mikov, M.,& Bugarčić, Z. M.. (2019). Discovery of the Biginelli hybrids as novel caspase-9 activators in apoptotic machines: Lipophilicity, molecular docking study, influence on angiogenesis gene and miR-21 expression levels. in Bioorganic Chemistry, 86, 569-582.
https://doi.org/10.1016/j.bioorg.2019.02.026

Janković NŽ, Trifunović Ristovski J, Vraneš M, Tot A, Petronijević J, Joksimović N, Stanojković TP, Đorđić Crnogorac M, Petrović N, Boljević I, Matić IZ, Bogdanović GA, Mikov M, Bugarčić ZM. Discovery of the Biginelli hybrids as novel caspase-9 activators in apoptotic machines: Lipophilicity, molecular docking study, influence on angiogenesis gene and miR-21 expression levels. in Bioorganic Chemistry. 2019;86:569-582.
doi:10.1016/j.bioorg.2019.02.026 .

Janković, Nenad Ž., Trifunović Ristovski, Jovana, Vraneš, Milan, Tot, Aleksandar, Petronijević, Jelena, Joksimović, Nenad, Stanojković, Tatjana P., Đorđić Crnogorac, Marija, Petrović, Nina, Boljević, Ivana, Matić, Ivana Z., Bogdanović, Goran A., Mikov, Momir, Bugarčić, Zorica M., "Discovery of the Biginelli hybrids as novel caspase-9 activators in apoptotic machines: Lipophilicity, molecular docking study, influence on angiogenesis gene and miR-21 expression levels" in Bioorganic Chemistry, 86 (2019):569-582,
https://doi.org/10.1016/j.bioorg.2019.02.026 . .

TY  - JOUR
AU  - Jakovljević, Katarina
AU  - Joksović, Milan D.
AU  - Matić, Ivana Z.
AU  - Petrović, Nina
AU  - Stanojković, Tatjana P.
AU  - Sladić, Dušan M.
AU  - Vujčić, Miroslava T.
AU  - Janović, Barbara S.
AU  - Joksović, Ljubinka G.
AU  - Trifunović, Snežana
AU  - Marković, Violeta
PY  - 2018
UR  - http://xlink.rsc.org/?DOI=C8MD00316E
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7928
AB  - Hybrid compounds that combine the 1,3,4-thiadiazole-containing catechol moiety with a chalcone motif were synthesized and examined for their antioxidant activity, cytotoxicity, and DNA-binding activity. A series of thirteen compounds showed strong antioxidant and cytotoxic effects on human acute promyelocytic leukemia HL-60 cells. Several compounds exerted good cytotoxic activities on cervical adenocarcinoma HeLa cells. The treatment of HeLa cells with IC50 and double IC50 concentrations of the compounds 5a, 5c, 5f, and 5m induced a statistically significant increase in the percentage of cells within a subG1 cell cycle phase. The examined compounds caused G2/M cell cycle arrest in HeLa cells. Each of these compounds triggered apoptosis in HeLa cells through activation of caspase-3, the main effector caspase, caspase-8, which is involved in the extrinsic apoptotic pathway, and caspase-9, which is involved in the intrinsic apoptotic pathway. All of the examined compounds decreased the expression levels of MMP2 in HeLa cells and levels of protumorigenic miR-133b. Compounds 5a and 5m lowered the expression level of oncogenic miR-21 in HeLa cells. In addition, compounds 5a, 5f, and 5m decreased the expression levels of oncogenic miR-155 while the treatment of HeLa cells with compounds 5a, 5c, and 5f increased expression of tumor-suppressive miR-206. Observed effects of these compounds on expression levels of four examined miRNAs suggest their prominent cancer-suppressive activity. An investigation by absorption and fluorescence spectroscopy showed more efficient calf thymus DNA binding activity of the compound 5m in comparison to other tested compounds. Results of a pUC19 plasmid cleavage study and comet assay showed DNA damaging activities of compounds 5a and 5c.
T2  - MedChemComm
T1  - Novel 1,3,4-thiadiazole–chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies
VL  - 9
IS  - 10
SP  - 1679
EP  - 1697
DO  - 10.1039/C8MD00316E
ER  - 

@article{
author = "Jakovljević, Katarina and Joksović, Milan D. and Matić, Ivana Z. and Petrović, Nina and Stanojković, Tatjana P. and Sladić, Dušan M. and Vujčić, Miroslava T. and Janović, Barbara S. and Joksović, Ljubinka G. and Trifunović, Snežana and Marković, Violeta",
year = "2018",
abstract = "Hybrid compounds that combine the 1,3,4-thiadiazole-containing catechol moiety with a chalcone motif were synthesized and examined for their antioxidant activity, cytotoxicity, and DNA-binding activity. A series of thirteen compounds showed strong antioxidant and cytotoxic effects on human acute promyelocytic leukemia HL-60 cells. Several compounds exerted good cytotoxic activities on cervical adenocarcinoma HeLa cells. The treatment of HeLa cells with IC50 and double IC50 concentrations of the compounds 5a, 5c, 5f, and 5m induced a statistically significant increase in the percentage of cells within a subG1 cell cycle phase. The examined compounds caused G2/M cell cycle arrest in HeLa cells. Each of these compounds triggered apoptosis in HeLa cells through activation of caspase-3, the main effector caspase, caspase-8, which is involved in the extrinsic apoptotic pathway, and caspase-9, which is involved in the intrinsic apoptotic pathway. All of the examined compounds decreased the expression levels of MMP2 in HeLa cells and levels of protumorigenic miR-133b. Compounds 5a and 5m lowered the expression level of oncogenic miR-21 in HeLa cells. In addition, compounds 5a, 5f, and 5m decreased the expression levels of oncogenic miR-155 while the treatment of HeLa cells with compounds 5a, 5c, and 5f increased expression of tumor-suppressive miR-206. Observed effects of these compounds on expression levels of four examined miRNAs suggest their prominent cancer-suppressive activity. An investigation by absorption and fluorescence spectroscopy showed more efficient calf thymus DNA binding activity of the compound 5m in comparison to other tested compounds. Results of a pUC19 plasmid cleavage study and comet assay showed DNA damaging activities of compounds 5a and 5c.",
journal = "MedChemComm",
title = "Novel 1,3,4-thiadiazole–chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies",
volume = "9",
number = "10",
pages = "1679-1697",
doi = "10.1039/C8MD00316E"
}

Jakovljević, K., Joksović, M. D., Matić, I. Z., Petrović, N., Stanojković, T. P., Sladić, D. M., Vujčić, M. T., Janović, B. S., Joksović, L. G., Trifunović, S.,& Marković, V.. (2018). Novel 1,3,4-thiadiazole–chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies. in MedChemComm, 9(10), 1679-1697.
https://doi.org/10.1039/C8MD00316E

Jakovljević K, Joksović MD, Matić IZ, Petrović N, Stanojković TP, Sladić DM, Vujčić MT, Janović BS, Joksović LG, Trifunović S, Marković V. Novel 1,3,4-thiadiazole–chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies. in MedChemComm. 2018;9(10):1679-1697.
doi:10.1039/C8MD00316E .

Jakovljević, Katarina, Joksović, Milan D., Matić, Ivana Z., Petrović, Nina, Stanojković, Tatjana P., Sladić, Dušan M., Vujčić, Miroslava T., Janović, Barbara S., Joksović, Ljubinka G., Trifunović, Snežana, Marković, Violeta, "Novel 1,3,4-thiadiazole–chalcone hybrids containing catechol moiety: synthesis, antioxidant activity, cytotoxicity and DNA interaction studies" in MedChemComm, 9, no. 10 (2018):1679-1697,
https://doi.org/10.1039/C8MD00316E . .

TY  - JOUR
AU  - Stanojković, Tatjana P.
AU  - Marković, Violeta
AU  - Matić, Ivana Z.
AU  - Mladenović, Milan P.
AU  - Petrović, Nina
AU  - Krivokuća, Ana M.
AU  - Petković, Miloš R.
AU  - Joksović, Milan D.
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0960894X18305493
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7815
AB  - A series of 23 novel anthraquinone-chalcone hybrids containing amide function was synthesized and structurally characterized. Sixteen compounds exerted strong cytotoxic activities against K562, Jurkat and HL-60 leukemia cell lines and significantly lower cytotoxic effects against normal MRC-5 cells, indicating very high selectivity in their anticancer action. The compounds 6g, 6u and 6v activate apoptosis in K562 cells through the extrinsic and intrinsic apoptotic pathway. The compound 6e triggered apoptosis in K562 cells only through the extrinsic apoptotic pathway. Treatment of K562 cells with each of these four compounds caused decrease in the expression levels of MMP2, MMP9, and VEGF, suggesting their anti-invasive, antimetastatic and antiangiogenic properties. The compounds 6g and 6v downregulated expression levels of miR-155 in K562 cells, while compounds 6e and 6u upregulated miR-155 levels in treated cells, in comparison with control cells. The structure-based 3-D QSAR models for 6f, 6e, 6i and 6l describe pro-apoptotic activity against caspase-3. © 2018 Elsevier Ltd
T2  - Bioorganic and Medicinal Chemistry Letters
T1  - Highly selective anthraquinone-chalcone hybrids as potential antileukemia agents
VL  - 28
IS  - 15
SP  - 2593
EP  - 2598
DO  - 10.1016/j.bmcl.2018.06.048
ER  - 

@article{
author = "Stanojković, Tatjana P. and Marković, Violeta and Matić, Ivana Z. and Mladenović, Milan P. and Petrović, Nina and Krivokuća, Ana M. and Petković, Miloš R. and Joksović, Milan D.",
year = "2018",
abstract = "A series of 23 novel anthraquinone-chalcone hybrids containing amide function was synthesized and structurally characterized. Sixteen compounds exerted strong cytotoxic activities against K562, Jurkat and HL-60 leukemia cell lines and significantly lower cytotoxic effects against normal MRC-5 cells, indicating very high selectivity in their anticancer action. The compounds 6g, 6u and 6v activate apoptosis in K562 cells through the extrinsic and intrinsic apoptotic pathway. The compound 6e triggered apoptosis in K562 cells only through the extrinsic apoptotic pathway. Treatment of K562 cells with each of these four compounds caused decrease in the expression levels of MMP2, MMP9, and VEGF, suggesting their anti-invasive, antimetastatic and antiangiogenic properties. The compounds 6g and 6v downregulated expression levels of miR-155 in K562 cells, while compounds 6e and 6u upregulated miR-155 levels in treated cells, in comparison with control cells. The structure-based 3-D QSAR models for 6f, 6e, 6i and 6l describe pro-apoptotic activity against caspase-3. © 2018 Elsevier Ltd",
journal = "Bioorganic and Medicinal Chemistry Letters",
title = "Highly selective anthraquinone-chalcone hybrids as potential antileukemia agents",
volume = "28",
number = "15",
pages = "2593-2598",
doi = "10.1016/j.bmcl.2018.06.048"
}

Stanojković, T. P., Marković, V., Matić, I. Z., Mladenović, M. P., Petrović, N., Krivokuća, A. M., Petković, M. R.,& Joksović, M. D.. (2018). Highly selective anthraquinone-chalcone hybrids as potential antileukemia agents. in Bioorganic and Medicinal Chemistry Letters, 28(15), 2593-2598.
https://doi.org/10.1016/j.bmcl.2018.06.048

Stanojković TP, Marković V, Matić IZ, Mladenović MP, Petrović N, Krivokuća AM, Petković MR, Joksović MD. Highly selective anthraquinone-chalcone hybrids as potential antileukemia agents. in Bioorganic and Medicinal Chemistry Letters. 2018;28(15):2593-2598.
doi:10.1016/j.bmcl.2018.06.048 .

Stanojković, Tatjana P., Marković, Violeta, Matić, Ivana Z., Mladenović, Milan P., Petrović, Nina, Krivokuća, Ana M., Petković, Miloš R., Joksović, Milan D., "Highly selective anthraquinone-chalcone hybrids as potential antileukemia agents" in Bioorganic and Medicinal Chemistry Letters, 28, no. 15 (2018):2593-2598,
https://doi.org/10.1016/j.bmcl.2018.06.048 . .

TY  - JOUR
AU  - Rodić, Marko V.
AU  - Leovac, Vukadin M.
AU  - Jovanović, Ljiljana S.
AU  - Spasojević, Vojislav
AU  - Joksović, Milan D.
AU  - Stanojković, Tatjana P.
AU  - Matić, Ivana Z.
AU  - Vojinović-Ješić, Ljiljana S.
AU  - Marković, Violeta
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1053
AB  - Three novel copper complexes with tridentate N2O ligand di(2-pyridil) ketone 1-adamantoyl hydrazone (Addpy) of the formula [(Cu2Cu2I)-Cu-II(Addpy)(2)Br-2(mu-Br-4)] (1), catena-poly[CuCl(mu-Addpy)(mu-Cl)CuCl2](n) (2) and [Cu(Addpy)(NCS)(2)] (3) were synthesized. Complexes are characterized by X-ray crystallography, spectral (UV-Vis, FTIR), electrochemical (CV) analyses, and magnetochemical measurements. Investigation of anticancer potential of Cu(II) complexes, mode of cell death, apoptosis, and inhibition of angiogenesis were performed. All tested malignant cell lines (HeLa, LS174, A549, K562, and MDA-MB-231) showed high sensitivity to the examined Cu(II) complexes. It has been shown that the complexes induce apoptosis in the caspase 3-dependent manner, whereas the anti-angiogenic effects of 1, 2, and 3 have been confirmed in EA.hy926 cells using a tube formation assay. (C) 2016 Elsevier Masson SAS. All rights reserved.
PB  - Elsevier
T2  - European Journal of Medicinal Chemistry
T1  - Synthesis, characterization, cytotoxicity and antiangiogenic activity of copper(II) complexes with 1-adamantoyl hydrazone bearing pyridine rings
VL  - 115
SP  - 75
EP  - 81
DO  - 10.1016/j.ejmech.2016.03.003
ER  - 

@article{
author = "Rodić, Marko V. and Leovac, Vukadin M. and Jovanović, Ljiljana S. and Spasojević, Vojislav and Joksović, Milan D. and Stanojković, Tatjana P. and Matić, Ivana Z. and Vojinović-Ješić, Ljiljana S. and Marković, Violeta",
year = "2016",
abstract = "Three novel copper complexes with tridentate N2O ligand di(2-pyridil) ketone 1-adamantoyl hydrazone (Addpy) of the formula [(Cu2Cu2I)-Cu-II(Addpy)(2)Br-2(mu-Br-4)] (1), catena-poly[CuCl(mu-Addpy)(mu-Cl)CuCl2](n) (2) and [Cu(Addpy)(NCS)(2)] (3) were synthesized. Complexes are characterized by X-ray crystallography, spectral (UV-Vis, FTIR), electrochemical (CV) analyses, and magnetochemical measurements. Investigation of anticancer potential of Cu(II) complexes, mode of cell death, apoptosis, and inhibition of angiogenesis were performed. All tested malignant cell lines (HeLa, LS174, A549, K562, and MDA-MB-231) showed high sensitivity to the examined Cu(II) complexes. It has been shown that the complexes induce apoptosis in the caspase 3-dependent manner, whereas the anti-angiogenic effects of 1, 2, and 3 have been confirmed in EA.hy926 cells using a tube formation assay. (C) 2016 Elsevier Masson SAS. All rights reserved.",
publisher = "Elsevier",
journal = "European Journal of Medicinal Chemistry",
title = "Synthesis, characterization, cytotoxicity and antiangiogenic activity of copper(II) complexes with 1-adamantoyl hydrazone bearing pyridine rings",
volume = "115",
pages = "75-81",
doi = "10.1016/j.ejmech.2016.03.003"
}

Rodić, M. V., Leovac, V. M., Jovanović, L. S., Spasojević, V., Joksović, M. D., Stanojković, T. P., Matić, I. Z., Vojinović-Ješić, L. S.,& Marković, V.. (2016). Synthesis, characterization, cytotoxicity and antiangiogenic activity of copper(II) complexes with 1-adamantoyl hydrazone bearing pyridine rings. in European Journal of Medicinal Chemistry
Elsevier., 115, 75-81.
https://doi.org/10.1016/j.ejmech.2016.03.003

Rodić MV, Leovac VM, Jovanović LS, Spasojević V, Joksović MD, Stanojković TP, Matić IZ, Vojinović-Ješić LS, Marković V. Synthesis, characterization, cytotoxicity and antiangiogenic activity of copper(II) complexes with 1-adamantoyl hydrazone bearing pyridine rings. in European Journal of Medicinal Chemistry. 2016;115:75-81.
doi:10.1016/j.ejmech.2016.03.003 .

Rodić, Marko V., Leovac, Vukadin M., Jovanović, Ljiljana S., Spasojević, Vojislav, Joksović, Milan D., Stanojković, Tatjana P., Matić, Ivana Z., Vojinović-Ješić, Ljiljana S., Marković, Violeta, "Synthesis, characterization, cytotoxicity and antiangiogenic activity of copper(II) complexes with 1-adamantoyl hydrazone bearing pyridine rings" in European Journal of Medicinal Chemistry, 115 (2016):75-81,
https://doi.org/10.1016/j.ejmech.2016.03.003 . .

TY  - JOUR
AU  - Surudžić, Rade D.
AU  - Janković, Ana
AU  - Mitrić, Miodrag
AU  - Matić, Ivana Z.
AU  - Juranić, Zorica D.
AU  - Živković, Ljiljana
AU  - Mišković-Stanković, Vesna B.
AU  - Rhee, Kyong Yop
AU  - Park, Soo Jin
AU  - Hui, David
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/933
AB  - Poly(vinyl alcohol)/graphene (PVA/Gr) nanocomposite was synthesized with the aim of developing a novel improved material. The PVA/Gr nanocomposite was characterized by UV-visible spectroscopy, cyclic voltammetry, Raman spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy analyses, and the interaction of PVA molecules with graphene was examined. Introduction of Gr led to improved mechanical and thermal properties of the PVA/Gr nanocomposite compared to pure PVA, as well as strong antibacterial activity against the bacterial strain Staphylococcus aureus. The PVA/Gr nanocomposite was non-cytotoxic against healthy peripheral blood mononuclear cells by MTT assay, indicating its high potential for biomedical applications. (C) 2015 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.
T2  - Journal of Industrial and Engineering Chemistry
T1  - The effect of graphene loading on mechanical, thermal and biological properties of poly(vinyl alcohol)/graphene nanocomposites
VL  - 34
SP  - 250
EP  - 257
DO  - 10.1016/j.jiec.2015.11.016
ER  - 

@article{
author = "Surudžić, Rade D. and Janković, Ana and Mitrić, Miodrag and Matić, Ivana Z. and Juranić, Zorica D. and Živković, Ljiljana and Mišković-Stanković, Vesna B. and Rhee, Kyong Yop and Park, Soo Jin and Hui, David",
year = "2016",
abstract = "Poly(vinyl alcohol)/graphene (PVA/Gr) nanocomposite was synthesized with the aim of developing a novel improved material. The PVA/Gr nanocomposite was characterized by UV-visible spectroscopy, cyclic voltammetry, Raman spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy analyses, and the interaction of PVA molecules with graphene was examined. Introduction of Gr led to improved mechanical and thermal properties of the PVA/Gr nanocomposite compared to pure PVA, as well as strong antibacterial activity against the bacterial strain Staphylococcus aureus. The PVA/Gr nanocomposite was non-cytotoxic against healthy peripheral blood mononuclear cells by MTT assay, indicating its high potential for biomedical applications. (C) 2015 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.",
journal = "Journal of Industrial and Engineering Chemistry",
title = "The effect of graphene loading on mechanical, thermal and biological properties of poly(vinyl alcohol)/graphene nanocomposites",
volume = "34",
pages = "250-257",
doi = "10.1016/j.jiec.2015.11.016"
}

Surudžić, R. D., Janković, A., Mitrić, M., Matić, I. Z., Juranić, Z. D., Živković, L., Mišković-Stanković, V. B., Rhee, K. Y., Park, S. J.,& Hui, D.. (2016). The effect of graphene loading on mechanical, thermal and biological properties of poly(vinyl alcohol)/graphene nanocomposites. in Journal of Industrial and Engineering Chemistry, 34, 250-257.
https://doi.org/10.1016/j.jiec.2015.11.016

Surudžić RD, Janković A, Mitrić M, Matić IZ, Juranić ZD, Živković L, Mišković-Stanković VB, Rhee KY, Park SJ, Hui D. The effect of graphene loading on mechanical, thermal and biological properties of poly(vinyl alcohol)/graphene nanocomposites. in Journal of Industrial and Engineering Chemistry. 2016;34:250-257.
doi:10.1016/j.jiec.2015.11.016 .

Surudžić, Rade D., Janković, Ana, Mitrić, Miodrag, Matić, Ivana Z., Juranić, Zorica D., Živković, Ljiljana, Mišković-Stanković, Vesna B., Rhee, Kyong Yop, Park, Soo Jin, Hui, David, "The effect of graphene loading on mechanical, thermal and biological properties of poly(vinyl alcohol)/graphene nanocomposites" in Journal of Industrial and Engineering Chemistry, 34 (2016):250-257,
https://doi.org/10.1016/j.jiec.2015.11.016 . .

TY  - JOUR
AU  - Janković, Ana
AU  - Eraković, Sanja
AU  - Mitrić, Miodrag
AU  - Matić, Ivana Z.
AU  - Juranić, Zorica D.
AU  - Tsui, Gary C. P.
AU  - Tang, Chak-yin
AU  - Mišković-Stanković, Vesna B.
AU  - Rhee, Kyong Yop
AU  - Park, Soo Jin
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/311
AB  - The hydroxyapatite/graphene (HAP/Gr) composite was electrodeposited on Ti using the electrophoretic deposition process to obtain uniform bioactive coating with improved mechanical strength and favorable corrosion stability in simulated body fluid (SBF). Incorporation of Gr was verified by Raman spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray photoelectron analysis. The HAP/Gr composite coating exhibited reduced surface cracks, nearly double the hardness, and elastic modulus increased by almost 50% compared to pure HAP coating, as estimated by a nanoindentation test. The bioactive HAP/Gr composite coating provided a newly formed apatite layer in SBF with enhanced corrosion stability, as evidenced by electrochemical impedance spectroscopy. The thermal stability of the HAP/Gr coating was improved in comparison to the pure HAP coating, and the Ca/P ratio was closer to the stoichiometric value. No antibacterial activity against Staphylococcus aureus or Escherichia coli could be verified. The HAP/Gr composite coating was classified as non-cytotoxic when tested against healthy peripheral blood mononuclear cells (PBMC). (C) 2014 Elsevier B.V. All rights reserved.
T2  - Journal of Alloys and Compounds
T1  - Bioactive hydroxyapatite/graphene composite coating and its corrosion stability in simulated body fluid
VL  - 624
SP  - 148
EP  - 157
DO  - 10.1016/j.jallcom.2014.11.078
ER  - 

@article{
author = "Janković, Ana and Eraković, Sanja and Mitrić, Miodrag and Matić, Ivana Z. and Juranić, Zorica D. and Tsui, Gary C. P. and Tang, Chak-yin and Mišković-Stanković, Vesna B. and Rhee, Kyong Yop and Park, Soo Jin",
year = "2015",
abstract = "The hydroxyapatite/graphene (HAP/Gr) composite was electrodeposited on Ti using the electrophoretic deposition process to obtain uniform bioactive coating with improved mechanical strength and favorable corrosion stability in simulated body fluid (SBF). Incorporation of Gr was verified by Raman spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray photoelectron analysis. The HAP/Gr composite coating exhibited reduced surface cracks, nearly double the hardness, and elastic modulus increased by almost 50% compared to pure HAP coating, as estimated by a nanoindentation test. The bioactive HAP/Gr composite coating provided a newly formed apatite layer in SBF with enhanced corrosion stability, as evidenced by electrochemical impedance spectroscopy. The thermal stability of the HAP/Gr coating was improved in comparison to the pure HAP coating, and the Ca/P ratio was closer to the stoichiometric value. No antibacterial activity against Staphylococcus aureus or Escherichia coli could be verified. The HAP/Gr composite coating was classified as non-cytotoxic when tested against healthy peripheral blood mononuclear cells (PBMC). (C) 2014 Elsevier B.V. All rights reserved.",
journal = "Journal of Alloys and Compounds",
title = "Bioactive hydroxyapatite/graphene composite coating and its corrosion stability in simulated body fluid",
volume = "624",
pages = "148-157",
doi = "10.1016/j.jallcom.2014.11.078"
}

Janković, A., Eraković, S., Mitrić, M., Matić, I. Z., Juranić, Z. D., Tsui, G. C. P., Tang, C., Mišković-Stanković, V. B., Rhee, K. Y.,& Park, S. J.. (2015). Bioactive hydroxyapatite/graphene composite coating and its corrosion stability in simulated body fluid. in Journal of Alloys and Compounds, 624, 148-157.
https://doi.org/10.1016/j.jallcom.2014.11.078

Janković A, Eraković S, Mitrić M, Matić IZ, Juranić ZD, Tsui GCP, Tang C, Mišković-Stanković VB, Rhee KY, Park SJ. Bioactive hydroxyapatite/graphene composite coating and its corrosion stability in simulated body fluid. in Journal of Alloys and Compounds. 2015;624:148-157.
doi:10.1016/j.jallcom.2014.11.078 .

Janković, Ana, Eraković, Sanja, Mitrić, Miodrag, Matić, Ivana Z., Juranić, Zorica D., Tsui, Gary C. P., Tang, Chak-yin, Mišković-Stanković, Vesna B., Rhee, Kyong Yop, Park, Soo Jin, "Bioactive hydroxyapatite/graphene composite coating and its corrosion stability in simulated body fluid" in Journal of Alloys and Compounds, 624 (2015):148-157,
https://doi.org/10.1016/j.jallcom.2014.11.078 . .

TY  - JOUR
AU  - Jovanović, Željka
AU  - Radosavljević, Aleksandra
AU  - Kačarević-Popović, Zorica M.
AU  - Stojkovska, Jasmina
AU  - Perić-Grujić, Aleksandra A.
AU  - Ristić, Mirjana
AU  - Matić, Ivana Z.
AU  - Juranić, Zorica D.
AU  - Obradović, Bojana
AU  - Mišković-Stanković, Vesna B.
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5379
AB  - Silver/poly(N-vinyl-2-pyrrolidone) (Ag/PVP) nanocomposites containing Ag nanoparticles at different concentrations were synthesized using gamma-irradiation. Cytotoxicity of the obtained nanocomposites was determined by MU assay in monolayer cultures of normal human immunocompetent peripheral blood mononuclear cells (PBMC) that were either non-stimulated or stimulated to proliferate by mitogen phytohemagglutinin (PHA), as well as in human cervix adenocarcinoma cell (HeLa) cultures. Silver release kinetics and mechanical properties of nanocomposites were investigated under bioreactor conditions in the simulated body fluid (SBF) at 37 degrees C. The release of silver was monitored under static conditions, and in two types of bioreactors: perfusion bioreactors and a bioreactor with dynamic compression coupled with SBF perfusion simulating in vivo conditions in articular cartilage. Ag/PVP nanocomposites exhibited slight cytotoxic effects against PBMC at the estimated concentration of 0.4 mu mol dm(-3), with negligible variations observed amongst different cell cultures investigated. Studies of the silver release kinetics indicated internal diffusion as the rate limiting step, determined by statistically comparable results obtained at all investigated conditions. However, silver release rate was slightly higher in the bioreactor with dynamic compression coupled with SBF perfusion as compared to the other two systems indicating the influence of dynamic compression. Modelling of silver release kinetics revealed potentials for optimization of Ag/PVP nanocomposites for particular applications as wound dressings or soft tissue implants. (C) 2013 Elsevier B.V. All rights reserved.
T2  - Colloids and Surfaces. B: Biointerfaces
T1  - Bioreactor validation and biocompatibility of Ag/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposites
VL  - 105
SP  - 230
EP  - 235
DO  - 10.1016/j.colsurfb.2012.12.055
ER  - 

@article{
author = "Jovanović, Željka and Radosavljević, Aleksandra and Kačarević-Popović, Zorica M. and Stojkovska, Jasmina and Perić-Grujić, Aleksandra A. and Ristić, Mirjana and Matić, Ivana Z. and Juranić, Zorica D. and Obradović, Bojana and Mišković-Stanković, Vesna B.",
year = "2013",
abstract = "Silver/poly(N-vinyl-2-pyrrolidone) (Ag/PVP) nanocomposites containing Ag nanoparticles at different concentrations were synthesized using gamma-irradiation. Cytotoxicity of the obtained nanocomposites was determined by MU assay in monolayer cultures of normal human immunocompetent peripheral blood mononuclear cells (PBMC) that were either non-stimulated or stimulated to proliferate by mitogen phytohemagglutinin (PHA), as well as in human cervix adenocarcinoma cell (HeLa) cultures. Silver release kinetics and mechanical properties of nanocomposites were investigated under bioreactor conditions in the simulated body fluid (SBF) at 37 degrees C. The release of silver was monitored under static conditions, and in two types of bioreactors: perfusion bioreactors and a bioreactor with dynamic compression coupled with SBF perfusion simulating in vivo conditions in articular cartilage. Ag/PVP nanocomposites exhibited slight cytotoxic effects against PBMC at the estimated concentration of 0.4 mu mol dm(-3), with negligible variations observed amongst different cell cultures investigated. Studies of the silver release kinetics indicated internal diffusion as the rate limiting step, determined by statistically comparable results obtained at all investigated conditions. However, silver release rate was slightly higher in the bioreactor with dynamic compression coupled with SBF perfusion as compared to the other two systems indicating the influence of dynamic compression. Modelling of silver release kinetics revealed potentials for optimization of Ag/PVP nanocomposites for particular applications as wound dressings or soft tissue implants. (C) 2013 Elsevier B.V. All rights reserved.",
journal = "Colloids and Surfaces. B: Biointerfaces",
title = "Bioreactor validation and biocompatibility of Ag/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposites",
volume = "105",
pages = "230-235",
doi = "10.1016/j.colsurfb.2012.12.055"
}

Jovanović, Ž., Radosavljević, A., Kačarević-Popović, Z. M., Stojkovska, J., Perić-Grujić, A. A., Ristić, M., Matić, I. Z., Juranić, Z. D., Obradović, B.,& Mišković-Stanković, V. B.. (2013). Bioreactor validation and biocompatibility of Ag/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposites. in Colloids and Surfaces. B: Biointerfaces, 105, 230-235.
https://doi.org/10.1016/j.colsurfb.2012.12.055

Jovanović Ž, Radosavljević A, Kačarević-Popović ZM, Stojkovska J, Perić-Grujić AA, Ristić M, Matić IZ, Juranić ZD, Obradović B, Mišković-Stanković VB. Bioreactor validation and biocompatibility of Ag/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposites. in Colloids and Surfaces. B: Biointerfaces. 2013;105:230-235.
doi:10.1016/j.colsurfb.2012.12.055 .

Jovanović, Željka, Radosavljević, Aleksandra, Kačarević-Popović, Zorica M., Stojkovska, Jasmina, Perić-Grujić, Aleksandra A., Ristić, Mirjana, Matić, Ivana Z., Juranić, Zorica D., Obradović, Bojana, Mišković-Stanković, Vesna B., "Bioreactor validation and biocompatibility of Ag/poly(N-vinyl-2-pyrrolidone) hydrogel nanocomposites" in Colloids and Surfaces. B: Biointerfaces, 105 (2013):230-235,
https://doi.org/10.1016/j.colsurfb.2012.12.055 . .

TY  - JOUR
AU  - Eraković, Sanja
AU  - Veljović, Đorđe N.
AU  - Diouf, Papa Niokhor
AU  - Stevanović, Tatjana
AU  - Mitrić, Miodrag
AU  - Janaćković, Đorđe T.
AU  - Matić, Ivana Z.
AU  - Juranić, Zorica D.
AU  - Mišković-Stanković, Vesna B.
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5089
AB  - Composite coatings based on lignin, obtained by electrophoretic deposition (EPD) on titanium, were investigated. The aim of this work was to produce hydroxyapatite/lignin (HAP/Lig) coatings on titanium and to investigate the effect of the lignin concentration on microstructure, morphology, phase composition, thermal behavior and cytotoxicity of the HAP/Lig coatings. An organosolv lignin was used for the production of the composite coatings studied in this research. The properties of HAP/Lig coatings were characterized using X-ray diffraction (XRD) analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FT-IR) and X-ray photoelectron spectroscopy (XPS), as well as the MTT test of cytotoxicity. The results showed that higher lignin concentrations protected the HAP lattice during sintering, thereby improving the stability of the HAP/Lig coatings. The cell survival of peripheral blood mononuclear cells (PBMC) after proliferation indicates that the HAP/Lig coating with 1 wt% Lig electrodeposited on titanium was non-toxic with significant promise as a potential bone-repair material. (C) 2012 Elsevier B.V. All rights reserved.
T2  - Progress in Organic Coatings
T1  - The effect of lignin on the structure and characteristics of composite coatings electrodeposited on titanium
VL  - 75
IS  - 4
SP  - 275
EP  - 283
DO  - 10.1016/j.porgcoat.2012.07.005
ER  - 

@article{
author = "Eraković, Sanja and Veljović, Đorđe N. and Diouf, Papa Niokhor and Stevanović, Tatjana and Mitrić, Miodrag and Janaćković, Đorđe T. and Matić, Ivana Z. and Juranić, Zorica D. and Mišković-Stanković, Vesna B.",
year = "2012",
abstract = "Composite coatings based on lignin, obtained by electrophoretic deposition (EPD) on titanium, were investigated. The aim of this work was to produce hydroxyapatite/lignin (HAP/Lig) coatings on titanium and to investigate the effect of the lignin concentration on microstructure, morphology, phase composition, thermal behavior and cytotoxicity of the HAP/Lig coatings. An organosolv lignin was used for the production of the composite coatings studied in this research. The properties of HAP/Lig coatings were characterized using X-ray diffraction (XRD) analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FT-IR) and X-ray photoelectron spectroscopy (XPS), as well as the MTT test of cytotoxicity. The results showed that higher lignin concentrations protected the HAP lattice during sintering, thereby improving the stability of the HAP/Lig coatings. The cell survival of peripheral blood mononuclear cells (PBMC) after proliferation indicates that the HAP/Lig coating with 1 wt% Lig electrodeposited on titanium was non-toxic with significant promise as a potential bone-repair material. (C) 2012 Elsevier B.V. All rights reserved.",
journal = "Progress in Organic Coatings",
title = "The effect of lignin on the structure and characteristics of composite coatings electrodeposited on titanium",
volume = "75",
number = "4",
pages = "275-283",
doi = "10.1016/j.porgcoat.2012.07.005"
}

Eraković, S., Veljović, Đ. N., Diouf, P. N., Stevanović, T., Mitrić, M., Janaćković, Đ. T., Matić, I. Z., Juranić, Z. D.,& Mišković-Stanković, V. B.. (2012). The effect of lignin on the structure and characteristics of composite coatings electrodeposited on titanium. in Progress in Organic Coatings, 75(4), 275-283.
https://doi.org/10.1016/j.porgcoat.2012.07.005

Eraković S, Veljović ĐN, Diouf PN, Stevanović T, Mitrić M, Janaćković ĐT, Matić IZ, Juranić ZD, Mišković-Stanković VB. The effect of lignin on the structure and characteristics of composite coatings electrodeposited on titanium. in Progress in Organic Coatings. 2012;75(4):275-283.
doi:10.1016/j.porgcoat.2012.07.005 .

Eraković, Sanja, Veljović, Đorđe N., Diouf, Papa Niokhor, Stevanović, Tatjana, Mitrić, Miodrag, Janaćković, Đorđe T., Matić, Ivana Z., Juranić, Zorica D., Mišković-Stanković, Vesna B., "The effect of lignin on the structure and characteristics of composite coatings electrodeposited on titanium" in Progress in Organic Coatings, 75, no. 4 (2012):275-283,
https://doi.org/10.1016/j.porgcoat.2012.07.005 . .