TY  - JOUR
AU  - Mandušić, Vesna
AU  - Popov-Celeketic, Dusan
AU  - Nešković-Konstantinović, Zora
AU  - Kanjer, Ksenija
AU  - Božović, Ana M.
AU  - Nikolić-Vukosavljević, Dragica
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4046
AB  - It is well known that breast tumors which are estrogen positive ER(+) are more likely to respond to hormone therapy. However, a certain percentage of ER(+)/PR(+) tumors do not respond to this therapy. Identification of the second estrogen receptor, named estrogen receptor beta (ER), as well as the existence of numerous isoforms/splice variants of both ER alpha and ER beta, suggests that a complex regulation of estrogen action exists. In this study, we analyzed the expression ratio of ER beta 1 isoform and ER beta Delta 5 splice variant mRNAs, and its correlation with ER/PR status by quantitative RT-PCR and clinical and histopathological parameters. We found that the relative proportion of ER beta Delta 5 in the total ER beta 1 transcript pool inversely correlates with the PR level (rho = -0,359, p LT 0,003, Spearman). It may be that the ER beta Delta 5 variant modulates the ERa activity of downstream targets. In addition, we suggest that the determination of the expression profiles of ER alpha and ER beta isoforms and splice variants in the defined groups of patients are necessary for elucidating their involvement in endocrine resistance.
T2  - Archives of Biological Sciences
T1  - Levels of Estrogen Receptor B Splice Variant (Erb Delta 5) Mrna Correlates with Progesterone Receptor in Breast Carcinomas
VL  - 62
IS  - 2
SP  - 257
EP  - 262
DO  - 10.2298/ABS1002257M
ER  - 

@article{
author = "Mandušić, Vesna and Popov-Celeketic, Dusan and Nešković-Konstantinović, Zora and Kanjer, Ksenija and Božović, Ana M. and Nikolić-Vukosavljević, Dragica",
year = "2010",
abstract = "It is well known that breast tumors which are estrogen positive ER(+) are more likely to respond to hormone therapy. However, a certain percentage of ER(+)/PR(+) tumors do not respond to this therapy. Identification of the second estrogen receptor, named estrogen receptor beta (ER), as well as the existence of numerous isoforms/splice variants of both ER alpha and ER beta, suggests that a complex regulation of estrogen action exists. In this study, we analyzed the expression ratio of ER beta 1 isoform and ER beta Delta 5 splice variant mRNAs, and its correlation with ER/PR status by quantitative RT-PCR and clinical and histopathological parameters. We found that the relative proportion of ER beta Delta 5 in the total ER beta 1 transcript pool inversely correlates with the PR level (rho = -0,359, p LT 0,003, Spearman). It may be that the ER beta Delta 5 variant modulates the ERa activity of downstream targets. In addition, we suggest that the determination of the expression profiles of ER alpha and ER beta isoforms and splice variants in the defined groups of patients are necessary for elucidating their involvement in endocrine resistance.",
journal = "Archives of Biological Sciences",
title = "Levels of Estrogen Receptor B Splice Variant (Erb Delta 5) Mrna Correlates with Progesterone Receptor in Breast Carcinomas",
volume = "62",
number = "2",
pages = "257-262",
doi = "10.2298/ABS1002257M"
}

Mandušić, V., Popov-Celeketic, D., Nešković-Konstantinović, Z., Kanjer, K., Božović, A. M.,& Nikolić-Vukosavljević, D.. (2010). Levels of Estrogen Receptor B Splice Variant (Erb Delta 5) Mrna Correlates with Progesterone Receptor in Breast Carcinomas. in Archives of Biological Sciences, 62(2), 257-262.
https://doi.org/10.2298/ABS1002257M

Mandušić V, Popov-Celeketic D, Nešković-Konstantinović Z, Kanjer K, Božović AM, Nikolić-Vukosavljević D. Levels of Estrogen Receptor B Splice Variant (Erb Delta 5) Mrna Correlates with Progesterone Receptor in Breast Carcinomas. in Archives of Biological Sciences. 2010;62(2):257-262.
doi:10.2298/ABS1002257M .

Mandušić, Vesna, Popov-Celeketic, Dusan, Nešković-Konstantinović, Zora, Kanjer, Ksenija, Božović, Ana M., Nikolić-Vukosavljević, Dragica, "Levels of Estrogen Receptor B Splice Variant (Erb Delta 5) Mrna Correlates with Progesterone Receptor in Breast Carcinomas" in Archives of Biological Sciences, 62, no. 2 (2010):257-262,
https://doi.org/10.2298/ABS1002257M . .

TY  - JOUR
AU  - Mandušić, Vesna
AU  - Krtolica-Žikić, Koviljka
AU  - Nikolić-Vukosavljević, Dragica
AU  - Popov-Celeketic, D.
AU  - Plećaš, D.
AU  - Boricic, I.
AU  - Dimitrijević, Bogomir B.
AU  - Tanić, Nikola
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3414
T2  - Archives of Biological Sciences
T1  - Transcriptional ratio of estrogen receptor beta mRNAs in carcinomas and in normal tissues.
VL  - 59
IS  - 2
SP  - 15P
EP  - 16P
DO  - 10.2298/ABS070215PM
ER  - 

@article{
author = "Mandušić, Vesna and Krtolica-Žikić, Koviljka and Nikolić-Vukosavljević, Dragica and Popov-Celeketic, D. and Plećaš, D. and Boricic, I. and Dimitrijević, Bogomir B. and Tanić, Nikola",
year = "2007",
journal = "Archives of Biological Sciences",
title = "Transcriptional ratio of estrogen receptor beta mRNAs in carcinomas and in normal tissues.",
volume = "59",
number = "2",
pages = "15P-16P",
doi = "10.2298/ABS070215PM"
}

Mandušić, V., Krtolica-Žikić, K., Nikolić-Vukosavljević, D., Popov-Celeketic, D., Plećaš, D., Boricic, I., Dimitrijević, B. B.,& Tanić, N.. (2007). Transcriptional ratio of estrogen receptor beta mRNAs in carcinomas and in normal tissues.. in Archives of Biological Sciences, 59(2), 15P-16P.
https://doi.org/10.2298/ABS070215PM

Mandušić V, Krtolica-Žikić K, Nikolić-Vukosavljević D, Popov-Celeketic D, Plećaš D, Boricic I, Dimitrijević BB, Tanić N. Transcriptional ratio of estrogen receptor beta mRNAs in carcinomas and in normal tissues.. in Archives of Biological Sciences. 2007;59(2):15P-16P.
doi:10.2298/ABS070215PM .

Mandušić, Vesna, Krtolica-Žikić, Koviljka, Nikolić-Vukosavljević, Dragica, Popov-Celeketic, D., Plećaš, D., Boricic, I., Dimitrijević, Bogomir B., Tanić, Nikola, "Transcriptional ratio of estrogen receptor beta mRNAs in carcinomas and in normal tissues." in Archives of Biological Sciences, 59, no. 2 (2007):15P-16P,
https://doi.org/10.2298/ABS070215PM . .

TY  - JOUR
AU  - Vranić-Mandušić, Vesna
AU  - Subota, Vesna
AU  - Savovski, K
AU  - Medic, L
AU  - Dramićanin, Tatjana
AU  - Jozanov-Stankov, Olga
AU  - Popov-Celeketic, D
AU  - Jokanović, Milan
AU  - Dimitrijević, Bogomir B.
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2701
AB  - Tiazofurin (TZF) is a cytostatic drug that leads to depletion of the GTP pool in tumor and normal cells via its active metabolite tiazofurin-adenine dinucleotide (TAD). TAD was detected in different cell lines, but not in erythrocytes, so the mechanism of erythrocytotoxicity of TZF remains unclear. The purpose of this study was to evaluate in vitro and in vivo action of tiazofurin on rat erythrocytes (RBC). After two decades of clinical trials the question of erythrocytotoxicity of TZF had remained unexplained making this study justified. Since we have previously demonstrated early erythrocytotoxic effects in male Wistar rats, we extend this finding on isolated RBC. Isolated erythrocytes from untreated animals were treated in buffered solution or plasma containing TZF In addition, groups of 10 rats were treated with 200 and 1000 mg/kg of TZF and hematologic parameters were analyzed by flowcytometry and by the analysis of the peripheral blood smears. Early signs of hemolysis or aberrant structures were monitored by scanning probe microscopy (SPM). We suggest that correlation exists between early erythrocytotoxicity and irregularities in erythrocyte morphology and membrane integrity. We also found that TZF affects responsiveness to oxidative stress. This is in concordance with flowcytometric findings describing anisocytosis and anisochromosis of RBC. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
T2  - Toxicology Letters
T1  - Erythrocytotoxicity of tiazofurin in vivo and in vitro detected by scanning probe microscopy
VL  - 146
IS  - 3
SP  - 275
EP  - 284
DO  - 10.1016/j.toxlet.2003.10.013
ER  - 

@article{
author = "Vranić-Mandušić, Vesna and Subota, Vesna and Savovski, K and Medic, L and Dramićanin, Tatjana and Jozanov-Stankov, Olga and Popov-Celeketic, D and Jokanović, Milan and Dimitrijević, Bogomir B.",
year = "2004",
abstract = "Tiazofurin (TZF) is a cytostatic drug that leads to depletion of the GTP pool in tumor and normal cells via its active metabolite tiazofurin-adenine dinucleotide (TAD). TAD was detected in different cell lines, but not in erythrocytes, so the mechanism of erythrocytotoxicity of TZF remains unclear. The purpose of this study was to evaluate in vitro and in vivo action of tiazofurin on rat erythrocytes (RBC). After two decades of clinical trials the question of erythrocytotoxicity of TZF had remained unexplained making this study justified. Since we have previously demonstrated early erythrocytotoxic effects in male Wistar rats, we extend this finding on isolated RBC. Isolated erythrocytes from untreated animals were treated in buffered solution or plasma containing TZF In addition, groups of 10 rats were treated with 200 and 1000 mg/kg of TZF and hematologic parameters were analyzed by flowcytometry and by the analysis of the peripheral blood smears. Early signs of hemolysis or aberrant structures were monitored by scanning probe microscopy (SPM). We suggest that correlation exists between early erythrocytotoxicity and irregularities in erythrocyte morphology and membrane integrity. We also found that TZF affects responsiveness to oxidative stress. This is in concordance with flowcytometric findings describing anisocytosis and anisochromosis of RBC. (C) 2003 Elsevier Ireland Ltd. All rights reserved.",
journal = "Toxicology Letters",
title = "Erythrocytotoxicity of tiazofurin in vivo and in vitro detected by scanning probe microscopy",
volume = "146",
number = "3",
pages = "275-284",
doi = "10.1016/j.toxlet.2003.10.013"
}

Vranić-Mandušić, V., Subota, V., Savovski, K., Medic, L., Dramićanin, T., Jozanov-Stankov, O., Popov-Celeketic, D., Jokanović, M.,& Dimitrijević, B. B.. (2004). Erythrocytotoxicity of tiazofurin in vivo and in vitro detected by scanning probe microscopy. in Toxicology Letters, 146(3), 275-284.
https://doi.org/10.1016/j.toxlet.2003.10.013

Vranić-Mandušić V, Subota V, Savovski K, Medic L, Dramićanin T, Jozanov-Stankov O, Popov-Celeketic D, Jokanović M, Dimitrijević BB. Erythrocytotoxicity of tiazofurin in vivo and in vitro detected by scanning probe microscopy. in Toxicology Letters. 2004;146(3):275-284.
doi:10.1016/j.toxlet.2003.10.013 .

Vranić-Mandušić, Vesna, Subota, Vesna, Savovski, K, Medic, L, Dramićanin, Tatjana, Jozanov-Stankov, Olga, Popov-Celeketic, D, Jokanović, Milan, Dimitrijević, Bogomir B., "Erythrocytotoxicity of tiazofurin in vivo and in vitro detected by scanning probe microscopy" in Toxicology Letters, 146, no. 3 (2004):275-284,
https://doi.org/10.1016/j.toxlet.2003.10.013 . .