TY  - JOUR
AU  - Velickovic, Natasa
AU  - Drakulić, Dunja R.
AU  - Petrovic, Snjezana
AU  - Grković, Ivana
AU  - Milošević, Maja
AU  - Stanojlović, Miloš R.
AU  - Horvat, Anica
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5051
AB  - Recent studies reported that exposure of juvenile rats to cranial irradiation affects hypothalamic-pituitary-adrenal (HPA) axis stability, leading to its activation along with radiation-induced inflammation. In the present study, we hypothesized whether inflammatory reaction in the CNS could be a mediator of HPA axis response to cranial irradiation (CI). Therefore, we analyzed time-course changes of serum corticosterone level, as well IL-1 beta and TNF-alpha level in the serum and hypothalamus of juvenile rats after CI. Protein and gene expression of the glucocorticoid receptor (GR) and nuclear factor kappaB (NF kappa B) were examined in the hippocampus within 24 h postirradiation interval. Cranial irradiation led to rapid induction of both GR and NF kappa B mRNA and protein in the hippocampus at 1 h. The increment in NF kappa B protein persisted for 2 h, therefore NF kappa B/GR protein ratio was turned in favor of NF kappa B. Central inflammation was characterized by increased IL-1 beta in the hypothalamus, with maximum levels at 2 and 4 h after irradiation, while both IL-1 beta and TNF-alpha were undetectable in the serum. Enhanced hypothalamic IL-1 beta probably induced the relocation of hippocampal NF kappa B to the nucleus and decreased NF kappa B mRNA at 6 h, indicating promotion of inflammation in the key tissue for HPA axis regulation. Concomitant increase of corticosterone level and enhanced GR nuclear translocation in the hippocampus at 6 h might represent a compensatory mechanism for observed inflammation. Our results indicate that acute radiation response is characterized by increased central inflammation and concomitant HPA axis activation, most likely having a role in protection of the organism from overwhelming inflammatory reaction.
T2  - Cellular and Molecular Neurobiology
T1  - Time-Course of Hypothalamic-Pituitary-Adrenal Axis Activity and Inflammation in Juvenile Rat Brain After Cranial Irradiation
VL  - 32
IS  - 7
SP  - 1175
EP  - 1185
DO  - 10.1007/s10571-012-9843-1
ER  - 

@article{
author = "Velickovic, Natasa and Drakulić, Dunja R. and Petrovic, Snjezana and Grković, Ivana and Milošević, Maja and Stanojlović, Miloš R. and Horvat, Anica",
year = "2012",
abstract = "Recent studies reported that exposure of juvenile rats to cranial irradiation affects hypothalamic-pituitary-adrenal (HPA) axis stability, leading to its activation along with radiation-induced inflammation. In the present study, we hypothesized whether inflammatory reaction in the CNS could be a mediator of HPA axis response to cranial irradiation (CI). Therefore, we analyzed time-course changes of serum corticosterone level, as well IL-1 beta and TNF-alpha level in the serum and hypothalamus of juvenile rats after CI. Protein and gene expression of the glucocorticoid receptor (GR) and nuclear factor kappaB (NF kappa B) were examined in the hippocampus within 24 h postirradiation interval. Cranial irradiation led to rapid induction of both GR and NF kappa B mRNA and protein in the hippocampus at 1 h. The increment in NF kappa B protein persisted for 2 h, therefore NF kappa B/GR protein ratio was turned in favor of NF kappa B. Central inflammation was characterized by increased IL-1 beta in the hypothalamus, with maximum levels at 2 and 4 h after irradiation, while both IL-1 beta and TNF-alpha were undetectable in the serum. Enhanced hypothalamic IL-1 beta probably induced the relocation of hippocampal NF kappa B to the nucleus and decreased NF kappa B mRNA at 6 h, indicating promotion of inflammation in the key tissue for HPA axis regulation. Concomitant increase of corticosterone level and enhanced GR nuclear translocation in the hippocampus at 6 h might represent a compensatory mechanism for observed inflammation. Our results indicate that acute radiation response is characterized by increased central inflammation and concomitant HPA axis activation, most likely having a role in protection of the organism from overwhelming inflammatory reaction.",
journal = "Cellular and Molecular Neurobiology",
title = "Time-Course of Hypothalamic-Pituitary-Adrenal Axis Activity and Inflammation in Juvenile Rat Brain After Cranial Irradiation",
volume = "32",
number = "7",
pages = "1175-1185",
doi = "10.1007/s10571-012-9843-1"
}

Velickovic, N., Drakulić, D. R., Petrovic, S., Grković, I., Milošević, M., Stanojlović, M. R.,& Horvat, A.. (2012). Time-Course of Hypothalamic-Pituitary-Adrenal Axis Activity and Inflammation in Juvenile Rat Brain After Cranial Irradiation. in Cellular and Molecular Neurobiology, 32(7), 1175-1185.
https://doi.org/10.1007/s10571-012-9843-1

Velickovic N, Drakulić DR, Petrovic S, Grković I, Milošević M, Stanojlović MR, Horvat A. Time-Course of Hypothalamic-Pituitary-Adrenal Axis Activity and Inflammation in Juvenile Rat Brain After Cranial Irradiation. in Cellular and Molecular Neurobiology. 2012;32(7):1175-1185.
doi:10.1007/s10571-012-9843-1 .

Velickovic, Natasa, Drakulić, Dunja R., Petrovic, Snjezana, Grković, Ivana, Milošević, Maja, Stanojlović, Miloš R., Horvat, Anica, "Time-Course of Hypothalamic-Pituitary-Adrenal Axis Activity and Inflammation in Juvenile Rat Brain After Cranial Irradiation" in Cellular and Molecular Neurobiology, 32, no. 7 (2012):1175-1185,
https://doi.org/10.1007/s10571-012-9843-1 . .

TY  - JOUR
AU  - Milošević, Maja
AU  - Petrovic, Snjezana
AU  - Velickovic, Natasa
AU  - Grković, Ivana
AU  - Ignjatović, Marija
AU  - Horvat, Anica
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5131
AB  - Extracellular nucleotides affect female reproductive functions, fertilization, and pregnancy. The aim of this study was to investigate biochemical characteristics of ATP and ADP hydrolysis and identify E-NTPDases in myometrial cell membranes from Wistar albino rats. The apparent K (m) values were 506.4 +/- A 62.1 and 638.8 +/- A 31.3 mu M, with a calculated V (max) (app) of 3,973.0 +/- A 279.5 and 2,853.9 +/- A 79.8 nmol/min/mg for ATP and ADP, respectively. The enzyme activity described here has common properties characteristic for NTPDases: divalent cation dependence; alkaline pH optimum for both substrates, insensitivity to some of classical ATPase inhibitors (ouabain, oligomycine, theophylline, levamisole) and significant inhibition by suramine and high concentration of sodium azides (5 mM). According to similar apparent K-m values for both substrates, the ATP/ADP hydrolysis ratio, and Chevillard competition plot, NTPDase1 is dominant ATP/ADP hydrolyzing enzyme in myometrial cell membranes. RT-PCR analysis revealed expression of three members of ectonucleoside triphosphate diphosphohydrolase family (NTPDase 1, 2, and 8) in rat uterus. These findings may further elucidate the role of NTPDases and ATP in reproductive physiology.
T2  - Molecular and Cellular Biochemistry
T1  - ATP and ADP hydrolysis in cell membranes from rat myometrium
VL  - 371
IS  - 1-2
SP  - 199
EP  - 208
DO  - 10.1007/s11010-012-1436-2
ER  - 

@article{
author = "Milošević, Maja and Petrovic, Snjezana and Velickovic, Natasa and Grković, Ivana and Ignjatović, Marija and Horvat, Anica",
year = "2012",
abstract = "Extracellular nucleotides affect female reproductive functions, fertilization, and pregnancy. The aim of this study was to investigate biochemical characteristics of ATP and ADP hydrolysis and identify E-NTPDases in myometrial cell membranes from Wistar albino rats. The apparent K (m) values were 506.4 +/- A 62.1 and 638.8 +/- A 31.3 mu M, with a calculated V (max) (app) of 3,973.0 +/- A 279.5 and 2,853.9 +/- A 79.8 nmol/min/mg for ATP and ADP, respectively. The enzyme activity described here has common properties characteristic for NTPDases: divalent cation dependence; alkaline pH optimum for both substrates, insensitivity to some of classical ATPase inhibitors (ouabain, oligomycine, theophylline, levamisole) and significant inhibition by suramine and high concentration of sodium azides (5 mM). According to similar apparent K-m values for both substrates, the ATP/ADP hydrolysis ratio, and Chevillard competition plot, NTPDase1 is dominant ATP/ADP hydrolyzing enzyme in myometrial cell membranes. RT-PCR analysis revealed expression of three members of ectonucleoside triphosphate diphosphohydrolase family (NTPDase 1, 2, and 8) in rat uterus. These findings may further elucidate the role of NTPDases and ATP in reproductive physiology.",
journal = "Molecular and Cellular Biochemistry",
title = "ATP and ADP hydrolysis in cell membranes from rat myometrium",
volume = "371",
number = "1-2",
pages = "199-208",
doi = "10.1007/s11010-012-1436-2"
}

Milošević, M., Petrovic, S., Velickovic, N., Grković, I., Ignjatović, M.,& Horvat, A.. (2012). ATP and ADP hydrolysis in cell membranes from rat myometrium. in Molecular and Cellular Biochemistry, 371(1-2), 199-208.
https://doi.org/10.1007/s11010-012-1436-2

Milošević M, Petrovic S, Velickovic N, Grković I, Ignjatović M, Horvat A. ATP and ADP hydrolysis in cell membranes from rat myometrium. in Molecular and Cellular Biochemistry. 2012;371(1-2):199-208.
doi:10.1007/s11010-012-1436-2 .

Milošević, Maja, Petrovic, Snjezana, Velickovic, Natasa, Grković, Ivana, Ignjatović, Marija, Horvat, Anica, "ATP and ADP hydrolysis in cell membranes from rat myometrium" in Molecular and Cellular Biochemistry, 371, no. 1-2 (2012):199-208,
https://doi.org/10.1007/s11010-012-1436-2 . .