TY  - JOUR
AU  - Srdić-Rajić, Tatjana
AU  - Metlaš, Radmila
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10137
AB  - Gene as the basic functional unit of DNA encodes information about the product such as protein. The majority of proteins realize function through protein-protein interactions involving short protein motifs. However, some proteins such as antibodies are established by the rearrangement of several (V-D-J) gene segments with the potential addition of nontemplated nucleotides that may change information encoded by the respective gene segment used. Antibody VH domain sequence analysis by ISM bioinformatics approach that is based on amino acids physicochemical features, enable to distinguish the contribution of the information encoded by VH gene or generated during VDJ gene recombination for antibody-antigen interaction. The data presented in this report revealed the significance of CDRH3 for the interaction of antibody specific for immunogenic molecules while CDRH3 contribution is minor for antibody interaction with nonimmunogenic molecules such as haptens and native mammalian dsDNA. Thus, paratopes might be located in the CDRH3 or VH regions.
T2  - Immunology Letters
T1  - Antibody VH domain sequence analysis by a bioinformatics approach based on electronic amino acid properties may help to predict paratop location
VL  - 241
SP  - 55
EP  - 57
DO  - 10.1016/j.imlet.2021.11.003
ER  - 

@article{
author = "Srdić-Rajić, Tatjana and Metlaš, Radmila",
year = "2022",
abstract = "Gene as the basic functional unit of DNA encodes information about the product such as protein. The majority of proteins realize function through protein-protein interactions involving short protein motifs. However, some proteins such as antibodies are established by the rearrangement of several (V-D-J) gene segments with the potential addition of nontemplated nucleotides that may change information encoded by the respective gene segment used. Antibody VH domain sequence analysis by ISM bioinformatics approach that is based on amino acids physicochemical features, enable to distinguish the contribution of the information encoded by VH gene or generated during VDJ gene recombination for antibody-antigen interaction. The data presented in this report revealed the significance of CDRH3 for the interaction of antibody specific for immunogenic molecules while CDRH3 contribution is minor for antibody interaction with nonimmunogenic molecules such as haptens and native mammalian dsDNA. Thus, paratopes might be located in the CDRH3 or VH regions.",
journal = "Immunology Letters",
title = "Antibody VH domain sequence analysis by a bioinformatics approach based on electronic amino acid properties may help to predict paratop location",
volume = "241",
pages = "55-57",
doi = "10.1016/j.imlet.2021.11.003"
}

Srdić-Rajić, T.,& Metlaš, R.. (2022). Antibody VH domain sequence analysis by a bioinformatics approach based on electronic amino acid properties may help to predict paratop location. in Immunology Letters, 241, 55-57.
https://doi.org/10.1016/j.imlet.2021.11.003

Srdić-Rajić T, Metlaš R. Antibody VH domain sequence analysis by a bioinformatics approach based on electronic amino acid properties may help to predict paratop location. in Immunology Letters. 2022;241:55-57.
doi:10.1016/j.imlet.2021.11.003 .

Srdić-Rajić, Tatjana, Metlaš, Radmila, "Antibody VH domain sequence analysis by a bioinformatics approach based on electronic amino acid properties may help to predict paratop location" in Immunology Letters, 241 (2022):55-57,
https://doi.org/10.1016/j.imlet.2021.11.003 . .

TY  - JOUR
AU  - Metlaš, Radmila
AU  - Srdić-Rajić, Tatjana
PY  - 2019
UR  - http://www.aidsreviews.com/resumen.php?id=1472
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8128
AB  - The goal of this report was to propose a model, wherein synergy between the B-cell antigen receptor (BCR) and toll-like receptor (TLR) signaling is involved in the selection of the B-cell precursors of HIV-1 broadly neutralizing antibodies (bnAbs) with long heavy chain complementarity determining regions 3, from immature/ transitional B cells. The model predicts the involvement of Ab/HIV-1 complexes in a way that Ab from the complex binds both BCRs and HIV-1, while on internalization of HIV-1 TLR ligands such as CpG motifs interacts with TLR9. The result of BCR and TLR9 orchestrated signaling is a formation of somatically mutated memory B cells potential precursors of bnAbs. Generated memory B cells continuously exposed to different Ab/HIV-1 complexes can elicit specific bnAb by stochastic somatic hypermutation rather than in the Darwinian process. This new view of the interaction between Ab/HIV-1 complexes and immune system, leading to affinity maturation of the bnAbs in the absence of nominal HIV-1 antigen and BCR interaction, may have implication for the vaccine designed and passive immunization. © 2019, Publicaciones Permanyer. All rights reserved.
T2  - Aids Reviews
T1  - A Model for Potential B-cell Precursors of Broadly Neutralizing HIV-1 Antibodies Selection and Antibody Affinity Maturation
VL  - 21
IS  - 1
SP  - 23
EP  - 27
DO  - 10.24875/AIDSRev.19000028
ER  - 

@article{
author = "Metlaš, Radmila and Srdić-Rajić, Tatjana",
year = "2019",
abstract = "The goal of this report was to propose a model, wherein synergy between the B-cell antigen receptor (BCR) and toll-like receptor (TLR) signaling is involved in the selection of the B-cell precursors of HIV-1 broadly neutralizing antibodies (bnAbs) with long heavy chain complementarity determining regions 3, from immature/ transitional B cells. The model predicts the involvement of Ab/HIV-1 complexes in a way that Ab from the complex binds both BCRs and HIV-1, while on internalization of HIV-1 TLR ligands such as CpG motifs interacts with TLR9. The result of BCR and TLR9 orchestrated signaling is a formation of somatically mutated memory B cells potential precursors of bnAbs. Generated memory B cells continuously exposed to different Ab/HIV-1 complexes can elicit specific bnAb by stochastic somatic hypermutation rather than in the Darwinian process. This new view of the interaction between Ab/HIV-1 complexes and immune system, leading to affinity maturation of the bnAbs in the absence of nominal HIV-1 antigen and BCR interaction, may have implication for the vaccine designed and passive immunization. © 2019, Publicaciones Permanyer. All rights reserved.",
journal = "Aids Reviews",
title = "A Model for Potential B-cell Precursors of Broadly Neutralizing HIV-1 Antibodies Selection and Antibody Affinity Maturation",
volume = "21",
number = "1",
pages = "23-27",
doi = "10.24875/AIDSRev.19000028"
}

Metlaš, R.,& Srdić-Rajić, T.. (2019). A Model for Potential B-cell Precursors of Broadly Neutralizing HIV-1 Antibodies Selection and Antibody Affinity Maturation. in Aids Reviews, 21(1), 23-27.
https://doi.org/10.24875/AIDSRev.19000028

Metlaš R, Srdić-Rajić T. A Model for Potential B-cell Precursors of Broadly Neutralizing HIV-1 Antibodies Selection and Antibody Affinity Maturation. in Aids Reviews. 2019;21(1):23-27.
doi:10.24875/AIDSRev.19000028 .

Metlaš, Radmila, Srdić-Rajić, Tatjana, "A Model for Potential B-cell Precursors of Broadly Neutralizing HIV-1 Antibodies Selection and Antibody Affinity Maturation" in Aids Reviews, 21, no. 1 (2019):23-27,
https://doi.org/10.24875/AIDSRev.19000028 . .

TY  - JOUR
AU  - Srdić-Rajić, Tatjana
AU  - Kohler, Heinz V.
AU  - Jurišić, Vladimir
AU  - Metlaš, Radmila
PY  - 2018
UR  - https://www.frontiersin.org/article/10.3389/fimmu.2018.02378/full
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7929
AB  - Analysis of protein sequences by the informational spectrum method (ISM) enables characterization of their specificity according to encoded information represented with defined frequency (F). Our previous data showed that F(0.367) is characteristic for variable heavy chain (VH) domains (a combination of variable (V), diversity (D) and joining (J) gene segments) of the anti-phosphocholine (PC) T15 antibodies and mostly dependent on the CDR2 region, a site for PC phosphate group binding. Because the T15 dsDNA-reactive U4 mutant also encodes F(0.367), we hypothesized that the same frequency may also be characteristic for anti-DNA antibodies. Data obtained from an analysis of 60 spontaneously produced anti-DNA antibody VH domain sequences supported our hypothesis only for antibodies, which use V gene segment in germline configuration, such as S57(VH31), MRL-DNA22, and VH11, members of the VH1 (J558) and VH7 (S107) gene families. The important finding is that out of seven V gene segments used by spontaneous anti-DNA antibodies, F(0.367) is only expressed by the germline configuration of these three V gene segments. The data suggest that antibody specificity for the phosphate group moiety delineated as F(0.367) is the intrinsic property of the V germline gene segments used, whereas paratope/epitope interaction with antigens bearing this epitope, such as PC or dsDNA, requires corresponding antibody VH conformation that is susceptible to somatic mutation(s). © 2018 Srdic-Rajic, Kohler, Jurisic and Metlas.
T2  - Frontiers in Immunology
T1  - Antibody Epitope Specificity for dsDNA Phosphate Backbone Is an Intrinsic Property of the Heavy Chain Variable Germline Gene Segment Used
VL  - 9
IS  - OCT
SP  - 2378
DO  - 10.3389/fimmu.2018.02378
ER  - 

@article{
author = "Srdić-Rajić, Tatjana and Kohler, Heinz V. and Jurišić, Vladimir and Metlaš, Radmila",
year = "2018",
abstract = "Analysis of protein sequences by the informational spectrum method (ISM) enables characterization of their specificity according to encoded information represented with defined frequency (F). Our previous data showed that F(0.367) is characteristic for variable heavy chain (VH) domains (a combination of variable (V), diversity (D) and joining (J) gene segments) of the anti-phosphocholine (PC) T15 antibodies and mostly dependent on the CDR2 region, a site for PC phosphate group binding. Because the T15 dsDNA-reactive U4 mutant also encodes F(0.367), we hypothesized that the same frequency may also be characteristic for anti-DNA antibodies. Data obtained from an analysis of 60 spontaneously produced anti-DNA antibody VH domain sequences supported our hypothesis only for antibodies, which use V gene segment in germline configuration, such as S57(VH31), MRL-DNA22, and VH11, members of the VH1 (J558) and VH7 (S107) gene families. The important finding is that out of seven V gene segments used by spontaneous anti-DNA antibodies, F(0.367) is only expressed by the germline configuration of these three V gene segments. The data suggest that antibody specificity for the phosphate group moiety delineated as F(0.367) is the intrinsic property of the V germline gene segments used, whereas paratope/epitope interaction with antigens bearing this epitope, such as PC or dsDNA, requires corresponding antibody VH conformation that is susceptible to somatic mutation(s). © 2018 Srdic-Rajic, Kohler, Jurisic and Metlas.",
journal = "Frontiers in Immunology",
title = "Antibody Epitope Specificity for dsDNA Phosphate Backbone Is an Intrinsic Property of the Heavy Chain Variable Germline Gene Segment Used",
volume = "9",
number = "OCT",
pages = "2378",
doi = "10.3389/fimmu.2018.02378"
}

Srdić-Rajić, T., Kohler, H. V., Jurišić, V.,& Metlaš, R.. (2018). Antibody Epitope Specificity for dsDNA Phosphate Backbone Is an Intrinsic Property of the Heavy Chain Variable Germline Gene Segment Used. in Frontiers in Immunology, 9(OCT), 2378.
https://doi.org/10.3389/fimmu.2018.02378

Srdić-Rajić T, Kohler HV, Jurišić V, Metlaš R. Antibody Epitope Specificity for dsDNA Phosphate Backbone Is an Intrinsic Property of the Heavy Chain Variable Germline Gene Segment Used. in Frontiers in Immunology. 2018;9(OCT):2378.
doi:10.3389/fimmu.2018.02378 .

Srdić-Rajić, Tatjana, Kohler, Heinz V., Jurišić, Vladimir, Metlaš, Radmila, "Antibody Epitope Specificity for dsDNA Phosphate Backbone Is an Intrinsic Property of the Heavy Chain Variable Germline Gene Segment Used" in Frontiers in Immunology, 9, no. OCT (2018):2378,
https://doi.org/10.3389/fimmu.2018.02378 . .

TY  - JOUR
AU  - Metlaš, Radmila
AU  - Srdić-Rajić, Tatjana
AU  - Kohler, Heike
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0165247818301147
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7793
AB  - The mechanisms for dominant T15 idiotype selection are not well understood, yet the significance of idiotypic regulation has been suggested. We proposed that to become dominant V regions of a given subset of B-1a cell must establish a functional idiotypic network with complementary T cells. Features required for the cells involved in immune network and steps preceding the establishment of clonal dominance are suggested. © 2018
T2  - Immunology Letters
T1  - Cooperation of intrathymic T15 idiotype-bearing B and complementary T cells in ontogeny of natural Treg cells involved in establishment of T15 clonal dominance
VL  - 200
SP  - 52
EP  - 54
DO  - 10.1016/j.imlet.2018.07.002
ER  - 

@article{
author = "Metlaš, Radmila and Srdić-Rajić, Tatjana and Kohler, Heike",
year = "2018",
abstract = "The mechanisms for dominant T15 idiotype selection are not well understood, yet the significance of idiotypic regulation has been suggested. We proposed that to become dominant V regions of a given subset of B-1a cell must establish a functional idiotypic network with complementary T cells. Features required for the cells involved in immune network and steps preceding the establishment of clonal dominance are suggested. © 2018",
journal = "Immunology Letters",
title = "Cooperation of intrathymic T15 idiotype-bearing B and complementary T cells in ontogeny of natural Treg cells involved in establishment of T15 clonal dominance",
volume = "200",
pages = "52-54",
doi = "10.1016/j.imlet.2018.07.002"
}

Metlaš, R., Srdić-Rajić, T.,& Kohler, H.. (2018). Cooperation of intrathymic T15 idiotype-bearing B and complementary T cells in ontogeny of natural Treg cells involved in establishment of T15 clonal dominance. in Immunology Letters, 200, 52-54.
https://doi.org/10.1016/j.imlet.2018.07.002

Metlaš R, Srdić-Rajić T, Kohler H. Cooperation of intrathymic T15 idiotype-bearing B and complementary T cells in ontogeny of natural Treg cells involved in establishment of T15 clonal dominance. in Immunology Letters. 2018;200:52-54.
doi:10.1016/j.imlet.2018.07.002 .

Metlaš, Radmila, Srdić-Rajić, Tatjana, Kohler, Heike, "Cooperation of intrathymic T15 idiotype-bearing B and complementary T cells in ontogeny of natural Treg cells involved in establishment of T15 clonal dominance" in Immunology Letters, 200 (2018):52-54,
https://doi.org/10.1016/j.imlet.2018.07.002 . .

TY  - JOUR
AU  - Srdić-Rajić, Tatjana
AU  - Keković, Goran
AU  - Davidović, Dragomir M.
AU  - Metlaš, Radmila
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5519
AB  - A physical and mathematical model identifies similarities between phosphocholine-binding antibodies.A methodology based on the representation of each amino acid of a protein sequence by the electron-ion interaction potential and subsequent analysis by signal processing was used to determine the characteristic or common frequency (in Hz) that reflects the biological activity shared among phosphocholine (PC)-binding antibodies. The common frequency for the variable portion of the heavy chain (VH) of the PC-specific antibodies is found to be at f 0.37 Hz. The VH sequences of the PC-binding antibodies exhibit three subsites for the PC moiety where hypervariable region 2 (CDR2) plays a role in the interaction with the phosphate group. Mutations in this VH region have an impact on the ability of mutant variants to bind PC and its carrier molecule, as well as on the characteristic frequency shift toward f 0.12 Hz for mutants failing to bind both hapten and carrier. The VH sequence of mutants that retain the ability to bind PC still shows f 0.37 Hz, suggesting that this frequency determines PC binding. However, this statement was not confirmed as mutation in another PC subsite impairs PC binding but retains both the phosphate-group recognition and the frequency at f 0.37 Hz. Herein, this finding is discussed to promote the idea that the VH sequence of the PC-binding antibodies encodes the subsite for phosphate-group binding as a dominant functional activity and that only CDR2 of the T15-idiotype antibodies together with FR3 region form an autonomous self-association function represented by the T15VH5073 peptide with f 0.370.05 Hz. Thus, these data confirmed that T15VH50-73 peptide might be used in superantibody technology.
T2  - International Immunology
T1  - Phosphocholine-binding antibody activities are hierarchically encoded in the sequence of the heavy-chain variable region: dominance of self-association activity in the T15 idiotype
VL  - 25
IS  - 6
SP  - 345
EP  - 352
DO  - 10.1093/intimm/dxs156
ER  - 

@article{
author = "Srdić-Rajić, Tatjana and Keković, Goran and Davidović, Dragomir M. and Metlaš, Radmila",
year = "2013",
abstract = "A physical and mathematical model identifies similarities between phosphocholine-binding antibodies.A methodology based on the representation of each amino acid of a protein sequence by the electron-ion interaction potential and subsequent analysis by signal processing was used to determine the characteristic or common frequency (in Hz) that reflects the biological activity shared among phosphocholine (PC)-binding antibodies. The common frequency for the variable portion of the heavy chain (VH) of the PC-specific antibodies is found to be at f 0.37 Hz. The VH sequences of the PC-binding antibodies exhibit three subsites for the PC moiety where hypervariable region 2 (CDR2) plays a role in the interaction with the phosphate group. Mutations in this VH region have an impact on the ability of mutant variants to bind PC and its carrier molecule, as well as on the characteristic frequency shift toward f 0.12 Hz for mutants failing to bind both hapten and carrier. The VH sequence of mutants that retain the ability to bind PC still shows f 0.37 Hz, suggesting that this frequency determines PC binding. However, this statement was not confirmed as mutation in another PC subsite impairs PC binding but retains both the phosphate-group recognition and the frequency at f 0.37 Hz. Herein, this finding is discussed to promote the idea that the VH sequence of the PC-binding antibodies encodes the subsite for phosphate-group binding as a dominant functional activity and that only CDR2 of the T15-idiotype antibodies together with FR3 region form an autonomous self-association function represented by the T15VH5073 peptide with f 0.370.05 Hz. Thus, these data confirmed that T15VH50-73 peptide might be used in superantibody technology.",
journal = "International Immunology",
title = "Phosphocholine-binding antibody activities are hierarchically encoded in the sequence of the heavy-chain variable region: dominance of self-association activity in the T15 idiotype",
volume = "25",
number = "6",
pages = "345-352",
doi = "10.1093/intimm/dxs156"
}

Srdić-Rajić, T., Keković, G., Davidović, D. M.,& Metlaš, R.. (2013). Phosphocholine-binding antibody activities are hierarchically encoded in the sequence of the heavy-chain variable region: dominance of self-association activity in the T15 idiotype. in International Immunology, 25(6), 345-352.
https://doi.org/10.1093/intimm/dxs156

Srdić-Rajić T, Keković G, Davidović DM, Metlaš R. Phosphocholine-binding antibody activities are hierarchically encoded in the sequence of the heavy-chain variable region: dominance of self-association activity in the T15 idiotype. in International Immunology. 2013;25(6):345-352.
doi:10.1093/intimm/dxs156 .

Srdić-Rajić, Tatjana, Keković, Goran, Davidović, Dragomir M., Metlaš, Radmila, "Phosphocholine-binding antibody activities are hierarchically encoded in the sequence of the heavy-chain variable region: dominance of self-association activity in the T15 idiotype" in International Immunology, 25, no. 6 (2013):345-352,
https://doi.org/10.1093/intimm/dxs156 . .

TY  - JOUR
AU  - Srdić-Rajić, Tatjana
AU  - Jurišić, Vladimir
AU  - Andrejevic, Sladjana
AU  - Bonaci-Nikolic, Branka
AU  - Bowker, Timothy
AU  - Concas, Daniela
AU  - Metlaš, Radmila
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4657
AB  - The production of autoantibodies against a vast array of self antigens, most notably double stranded (ds) DNA, characterized systemic lupus erythematosus (SLE). The purpose of this work is to study specific Ig fractions isolated from normal human serum (NHS) and their effect on the binding of anti-double-stranded deoxyribonucleic acid (dsDNA) antibodies (Abs) to dsDNA. A fraction named immunoglobulin G (IgG)-reactive IgG was purified from total NHS IgG by absorption onto (CNBr)-activated Sepharose 4B linked to intact IgG molecules (IgG-Sepharose column). IgG-reactive IgG was co-incubated with systemic lupus erythematosus (SLE) patients serum and binding of the anti-dsDNA Abs to dsDNA was measured by enzyme-linked immunosorbent assay (ELISA). Co-incubation of SLE patients serum with IgG-reactive IgG resulted in a dose-dependent reduction in binding of anti-dsDNA Abs to dsDNA. A reduction greater than 70% was observed at a concentration of 300 mu g of IgG-reactive IgG per mL of a 400-fold diluted SLE patients serum whereas total NHS IgG, at the same concentration, resulted in a 10% reduction in binding. The purification process used to isolate IgG-reactive IgG was based on interactions between intact Ig rather than on interactions between F(ab)(2) portions. IgG(2) is the predominant immunoglobulin (Ig) subclass in IgG-reactive IgG. Thus, IgG(2) might have an important role in the connectivity characteristics of NHS IgG. The capacity of IgG-reactive IgG to inhibit anti-DNA Ab binding to dsDNA may have potential application in the treatment of SLE. This targeted biological approach may provide an alternative strategy to immunosuppressants. (C) 2011 Elsevier GmbH. All rights reserved.
T2  - Immunobiology
T1  - Naturally occurring V region connected antibodies inhibit anti-dsDNA antibody reactivity with dsDNA
VL  - 217
IS  - 1
SP  - 111
EP  - 117
DO  - 10.1016/j.imbio.2011.07.026
ER  - 

@article{
author = "Srdić-Rajić, Tatjana and Jurišić, Vladimir and Andrejevic, Sladjana and Bonaci-Nikolic, Branka and Bowker, Timothy and Concas, Daniela and Metlaš, Radmila",
year = "2012",
abstract = "The production of autoantibodies against a vast array of self antigens, most notably double stranded (ds) DNA, characterized systemic lupus erythematosus (SLE). The purpose of this work is to study specific Ig fractions isolated from normal human serum (NHS) and their effect on the binding of anti-double-stranded deoxyribonucleic acid (dsDNA) antibodies (Abs) to dsDNA. A fraction named immunoglobulin G (IgG)-reactive IgG was purified from total NHS IgG by absorption onto (CNBr)-activated Sepharose 4B linked to intact IgG molecules (IgG-Sepharose column). IgG-reactive IgG was co-incubated with systemic lupus erythematosus (SLE) patients serum and binding of the anti-dsDNA Abs to dsDNA was measured by enzyme-linked immunosorbent assay (ELISA). Co-incubation of SLE patients serum with IgG-reactive IgG resulted in a dose-dependent reduction in binding of anti-dsDNA Abs to dsDNA. A reduction greater than 70% was observed at a concentration of 300 mu g of IgG-reactive IgG per mL of a 400-fold diluted SLE patients serum whereas total NHS IgG, at the same concentration, resulted in a 10% reduction in binding. The purification process used to isolate IgG-reactive IgG was based on interactions between intact Ig rather than on interactions between F(ab)(2) portions. IgG(2) is the predominant immunoglobulin (Ig) subclass in IgG-reactive IgG. Thus, IgG(2) might have an important role in the connectivity characteristics of NHS IgG. The capacity of IgG-reactive IgG to inhibit anti-DNA Ab binding to dsDNA may have potential application in the treatment of SLE. This targeted biological approach may provide an alternative strategy to immunosuppressants. (C) 2011 Elsevier GmbH. All rights reserved.",
journal = "Immunobiology",
title = "Naturally occurring V region connected antibodies inhibit anti-dsDNA antibody reactivity with dsDNA",
volume = "217",
number = "1",
pages = "111-117",
doi = "10.1016/j.imbio.2011.07.026"
}

Srdić-Rajić, T., Jurišić, V., Andrejevic, S., Bonaci-Nikolic, B., Bowker, T., Concas, D.,& Metlaš, R.. (2012). Naturally occurring V region connected antibodies inhibit anti-dsDNA antibody reactivity with dsDNA. in Immunobiology, 217(1), 111-117.
https://doi.org/10.1016/j.imbio.2011.07.026

Srdić-Rajić T, Jurišić V, Andrejevic S, Bonaci-Nikolic B, Bowker T, Concas D, Metlaš R. Naturally occurring V region connected antibodies inhibit anti-dsDNA antibody reactivity with dsDNA. in Immunobiology. 2012;217(1):111-117.
doi:10.1016/j.imbio.2011.07.026 .

Srdić-Rajić, Tatjana, Jurišić, Vladimir, Andrejevic, Sladjana, Bonaci-Nikolic, Branka, Bowker, Timothy, Concas, Daniela, Metlaš, Radmila, "Naturally occurring V region connected antibodies inhibit anti-dsDNA antibody reactivity with dsDNA" in Immunobiology, 217, no. 1 (2012):111-117,
https://doi.org/10.1016/j.imbio.2011.07.026 . .

TY  - JOUR
AU  - Metlaš, Radmila
AU  - Srdić, Tatjana
AU  - Veljković, Veljko
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3168
AB  - Immunoglobulins (Ig) of pooled healthy human sera were purified by affinity chromatography based on their reactivity with human IgG. This Ig fraction represent connected, natural antibodies (NAbs) and here are denoted as antiIgG antibodies. The data revealed that IgG, IgA and IgM isotypes arc constituents of anti-IgG fraction. The ability of antiIgG antibodies to prevent infection of PBMC by HIV-1 was demonstrated. They exhibited different neutralizing activity depending on the phenotype of the tested virus. The efficacy of neutralization was comparable to monoclonal antibodies (MAbs) IgG1b12 at least for the HIV-1 (92HT593B) Strain. These studies suggest that connected antibodies thus, constituents of immune network, could prevent infection by HIV-1. NAbs as essential components of therapeutic molecules of intravenous Ig (IVIg) have a beneficial effect on variety of immunological disorders by affecting the structure, function and dynamics of the immune network. Since, hallmark of HIV-1 infection are immunological disorders we hypothesizes that they might be corrected to some extend by anti-IgG antibodies.
T2  - Current HIV Research
T1  - Anti-IgG antibodies from sera of healthy individuals neutralize HIV-1 primary isolates
VL  - 5
IS  - 2
SP  - 261
EP  - 265
DO  - 10.2174/157016207780077093
ER  - 

@article{
author = "Metlaš, Radmila and Srdić, Tatjana and Veljković, Veljko",
year = "2007",
abstract = "Immunoglobulins (Ig) of pooled healthy human sera were purified by affinity chromatography based on their reactivity with human IgG. This Ig fraction represent connected, natural antibodies (NAbs) and here are denoted as antiIgG antibodies. The data revealed that IgG, IgA and IgM isotypes arc constituents of anti-IgG fraction. The ability of antiIgG antibodies to prevent infection of PBMC by HIV-1 was demonstrated. They exhibited different neutralizing activity depending on the phenotype of the tested virus. The efficacy of neutralization was comparable to monoclonal antibodies (MAbs) IgG1b12 at least for the HIV-1 (92HT593B) Strain. These studies suggest that connected antibodies thus, constituents of immune network, could prevent infection by HIV-1. NAbs as essential components of therapeutic molecules of intravenous Ig (IVIg) have a beneficial effect on variety of immunological disorders by affecting the structure, function and dynamics of the immune network. Since, hallmark of HIV-1 infection are immunological disorders we hypothesizes that they might be corrected to some extend by anti-IgG antibodies.",
journal = "Current HIV Research",
title = "Anti-IgG antibodies from sera of healthy individuals neutralize HIV-1 primary isolates",
volume = "5",
number = "2",
pages = "261-265",
doi = "10.2174/157016207780077093"
}

Metlaš, R., Srdić, T.,& Veljković, V.. (2007). Anti-IgG antibodies from sera of healthy individuals neutralize HIV-1 primary isolates. in Current HIV Research, 5(2), 261-265.
https://doi.org/10.2174/157016207780077093

Metlaš R, Srdić T, Veljković V. Anti-IgG antibodies from sera of healthy individuals neutralize HIV-1 primary isolates. in Current HIV Research. 2007;5(2):261-265.
doi:10.2174/157016207780077093 .

Metlaš, Radmila, Srdić, Tatjana, Veljković, Veljko, "Anti-IgG antibodies from sera of healthy individuals neutralize HIV-1 primary isolates" in Current HIV Research, 5, no. 2 (2007):261-265,
https://doi.org/10.2174/157016207780077093 . .

TY  - JOUR
AU  - Veljković, Nevena V.
AU  - Branch, DR
AU  - Metlaš, Radmila
AU  - Prljić, Jelena
AU  - Manfredi, R
AU  - Stringer, WW
AU  - Veljković, Veljko
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2837
AB  - It has been reported that antibodies reactive with peptide RSANFTDNAKTIIVQLNQSVEIN (peptide NTM) derived from the C-terminus of the second conserved domain of HIV-1 envelope glycoprotein gp120 could represent an important factor in control of the HIV disease. In order to check this notion we (i) tested reactivity with peptide NTM serum samples collected from 310 consecutive HIV-1 infected patients with a CD4(+) lymphocyte count ranging from 10 to 800/muL and (ii) performed the longitudinal study that included 107 sera samples collected from 29 HIV patients. Results of these studies demonstrated correlation between presence of anti-NTM antibodies in sera of HIV patients and disease progression measured by the CD4(+) cell count. Based on these findings we proposed the anti-NTM antibodies as useful prognostic marker for HIV disease. (C) 2004 Elsevier B.V. All rights reserved.
T2  - Journal of Clinical Virology
T1  - Antibodies reactive with C-terminus of the second conserved region of HIV-1gp120 as possible prognostic marker and therapeutic agent for HIV disease
VL  - 31
SP  - S39
EP  - S44
DO  - 10.1016/j.jcv.2004.09.012
ER  - 

@article{
author = "Veljković, Nevena V. and Branch, DR and Metlaš, Radmila and Prljić, Jelena and Manfredi, R and Stringer, WW and Veljković, Veljko",
year = "2004",
abstract = "It has been reported that antibodies reactive with peptide RSANFTDNAKTIIVQLNQSVEIN (peptide NTM) derived from the C-terminus of the second conserved domain of HIV-1 envelope glycoprotein gp120 could represent an important factor in control of the HIV disease. In order to check this notion we (i) tested reactivity with peptide NTM serum samples collected from 310 consecutive HIV-1 infected patients with a CD4(+) lymphocyte count ranging from 10 to 800/muL and (ii) performed the longitudinal study that included 107 sera samples collected from 29 HIV patients. Results of these studies demonstrated correlation between presence of anti-NTM antibodies in sera of HIV patients and disease progression measured by the CD4(+) cell count. Based on these findings we proposed the anti-NTM antibodies as useful prognostic marker for HIV disease. (C) 2004 Elsevier B.V. All rights reserved.",
journal = "Journal of Clinical Virology",
title = "Antibodies reactive with C-terminus of the second conserved region of HIV-1gp120 as possible prognostic marker and therapeutic agent for HIV disease",
volume = "31",
pages = "S39-S44",
doi = "10.1016/j.jcv.2004.09.012"
}

Veljković, N. V., Branch, D., Metlaš, R., Prljić, J., Manfredi, R., Stringer, W.,& Veljković, V.. (2004). Antibodies reactive with C-terminus of the second conserved region of HIV-1gp120 as possible prognostic marker and therapeutic agent for HIV disease. in Journal of Clinical Virology, 31, S39-S44.
https://doi.org/10.1016/j.jcv.2004.09.012

Veljković NV, Branch D, Metlaš R, Prljić J, Manfredi R, Stringer W, Veljković V. Antibodies reactive with C-terminus of the second conserved region of HIV-1gp120 as possible prognostic marker and therapeutic agent for HIV disease. in Journal of Clinical Virology. 2004;31:S39-S44.
doi:10.1016/j.jcv.2004.09.012 .

Veljković, Nevena V., Branch, DR, Metlaš, Radmila, Prljić, Jelena, Manfredi, R, Stringer, WW, Veljković, Veljko, "Antibodies reactive with C-terminus of the second conserved region of HIV-1gp120 as possible prognostic marker and therapeutic agent for HIV disease" in Journal of Clinical Virology, 31 (2004):S39-S44,
https://doi.org/10.1016/j.jcv.2004.09.012 . .

TY  - JOUR
AU  - Veljković, Veljko
AU  - Metlaš, Radmila
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2807
AB  - In sera of HIV-infected individuals natural antibodies recognizing nonimmunogenic C-terminal domain of the second conserved region of HIV-1 gp120 and the vasoactive intestinal peptide (VIP) were identified. It has been demonstrated that these antibodies are significantly more prevalent in asymptomatic carriers than in AIDS patients and that their titer strongly correlates with disease progression. These findings point out the VIP/C2-reactive natural antibodies as an important agent for immunotherapy of HIV disease.
T2  - International Reviews of Immunology
T1  - Application of VIP/NTM-reactive natural antibodies in therapy of HIV disease
VL  - 23
IS  - 5-6
SP  - 437
EP  - 445
DO  - 10.1080/08830180490432820
ER  - 

@article{
author = "Veljković, Veljko and Metlaš, Radmila",
year = "2004",
abstract = "In sera of HIV-infected individuals natural antibodies recognizing nonimmunogenic C-terminal domain of the second conserved region of HIV-1 gp120 and the vasoactive intestinal peptide (VIP) were identified. It has been demonstrated that these antibodies are significantly more prevalent in asymptomatic carriers than in AIDS patients and that their titer strongly correlates with disease progression. These findings point out the VIP/C2-reactive natural antibodies as an important agent for immunotherapy of HIV disease.",
journal = "International Reviews of Immunology",
title = "Application of VIP/NTM-reactive natural antibodies in therapy of HIV disease",
volume = "23",
number = "5-6",
pages = "437-445",
doi = "10.1080/08830180490432820"
}

Veljković, V.,& Metlaš, R.. (2004). Application of VIP/NTM-reactive natural antibodies in therapy of HIV disease. in International Reviews of Immunology, 23(5-6), 437-445.
https://doi.org/10.1080/08830180490432820

Veljković V, Metlaš R. Application of VIP/NTM-reactive natural antibodies in therapy of HIV disease. in International Reviews of Immunology. 2004;23(5-6):437-445.
doi:10.1080/08830180490432820 .

Veljković, Veljko, Metlaš, Radmila, "Application of VIP/NTM-reactive natural antibodies in therapy of HIV disease" in International Reviews of Immunology, 23, no. 5-6 (2004):437-445,
https://doi.org/10.1080/08830180490432820 . .

TY  - JOUR
AU  - Metlaš, Radmila
AU  - Veljković, Veljko
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2806
AB  - It has been demonstrated that the immunodominant V3 loop of HIV-1 gp120 and its flanking regions bear sequence and structural homology to the framework and complementarity-determining regions of human immunoglobulins. It has been proposed that the Ig-like domain of gp120 might encode idiotypes and in this way permit HIV-1 entry into the immune regulatory network. This notion is strongly supported by results demonstrating that the anti-V3 loop and anti-Ig antibodies of healthy individuals share complementary structure and that V3 reactive antibodies are present in HIV-negative sera. This might be the mechanism by which HIV induces immunological abnormalities, and it should be taken into consideration in AIDS vaccine development.
T2  - International Reviews of Immunology
T1  - HIV-1 gp120 and immune network
VL  - 23
IS  - 5-6
SP  - 413
EP  - 422
DO  - 10.1080/08830180490432758
ER  - 

@article{
author = "Metlaš, Radmila and Veljković, Veljko",
year = "2004",
abstract = "It has been demonstrated that the immunodominant V3 loop of HIV-1 gp120 and its flanking regions bear sequence and structural homology to the framework and complementarity-determining regions of human immunoglobulins. It has been proposed that the Ig-like domain of gp120 might encode idiotypes and in this way permit HIV-1 entry into the immune regulatory network. This notion is strongly supported by results demonstrating that the anti-V3 loop and anti-Ig antibodies of healthy individuals share complementary structure and that V3 reactive antibodies are present in HIV-negative sera. This might be the mechanism by which HIV induces immunological abnormalities, and it should be taken into consideration in AIDS vaccine development.",
journal = "International Reviews of Immunology",
title = "HIV-1 gp120 and immune network",
volume = "23",
number = "5-6",
pages = "413-422",
doi = "10.1080/08830180490432758"
}

Metlaš, R.,& Veljković, V.. (2004). HIV-1 gp120 and immune network. in International Reviews of Immunology, 23(5-6), 413-422.
https://doi.org/10.1080/08830180490432758

Metlaš R, Veljković V. HIV-1 gp120 and immune network. in International Reviews of Immunology. 2004;23(5-6):413-422.
doi:10.1080/08830180490432758 .

Metlaš, Radmila, Veljković, Veljko, "HIV-1 gp120 and immune network" in International Reviews of Immunology, 23, no. 5-6 (2004):413-422,
https://doi.org/10.1080/08830180490432758 . .

TY  - JOUR
AU  - Veljković, Veljko
AU  - Veljković, Nevena V.
AU  - Metlaš, Radmila
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2805
AB  - A broad range of structural, functional, and immunological similarities between HIV-1 gp120 and human proteins, especially those participating in immune responses, highlight gp120 as a pleiotropic protein that can in different ways affect many important functions of the human immune system. Here we described some of these properties of HIV-1 gp120 that represent the main obstacle in the development of effective and safe AIDS vaccine.
T2  - International Reviews of Immunology
T1  - Molecular makeup of HIV-1 envelope protein
VL  - 23
IS  - 5-6
SP  - 383
EP  - 411
DO  - 10.1080/08830180490432749
ER  - 

@article{
author = "Veljković, Veljko and Veljković, Nevena V. and Metlaš, Radmila",
year = "2004",
abstract = "A broad range of structural, functional, and immunological similarities between HIV-1 gp120 and human proteins, especially those participating in immune responses, highlight gp120 as a pleiotropic protein that can in different ways affect many important functions of the human immune system. Here we described some of these properties of HIV-1 gp120 that represent the main obstacle in the development of effective and safe AIDS vaccine.",
journal = "International Reviews of Immunology",
title = "Molecular makeup of HIV-1 envelope protein",
volume = "23",
number = "5-6",
pages = "383-411",
doi = "10.1080/08830180490432749"
}

Veljković, V., Veljković, N. V.,& Metlaš, R.. (2004). Molecular makeup of HIV-1 envelope protein. in International Reviews of Immunology, 23(5-6), 383-411.
https://doi.org/10.1080/08830180490432749

Veljković V, Veljković NV, Metlaš R. Molecular makeup of HIV-1 envelope protein. in International Reviews of Immunology. 2004;23(5-6):383-411.
doi:10.1080/08830180490432749 .

Veljković, Veljko, Veljković, Nevena V., Metlaš, Radmila, "Molecular makeup of HIV-1 envelope protein" in International Reviews of Immunology, 23, no. 5-6 (2004):383-411,
https://doi.org/10.1080/08830180490432749 . .

TY  - JOUR
AU  - Veljković, Nevena V.
AU  - Branch, DR
AU  - Metlaš, Radmila
AU  - Prljić, Jelena
AU  - Vlahovicek, K
AU  - Pongor, S
AU  - Veljković, Veljko
PY  - 2003
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2693
AB  - It has been reported that the C-terminus of the second conserved region (C2) of the envelope glycoprotein gp120, encompassing peptide RSANFTDNAKTIIVQLNESVEIN (NTM), is important for infectivity and neutralization of the human immunodeficiency virus type 1 (HIV-1). It was also demonstrated that human natural anti-vasoactive intestinal peptide (VIP) antibodies reactive with this gp120 region play an important role in control of HIV disease progression. The bioinformatic analysis based on the time-frequency signal processing revealed non-obvious similarities between NTM and VIP. When tested against a battery of sera from 46 AIDS patients, these peptides, in spite of a significant difference in their primary structures, showed a similar reactivity profiles (r = 0.83). Presented results point out that similarity in the periodical pattern of some physicochemical properties in primary structures of peptides plays a significant role in determination of their immunological crossreactivity. Based on these findings, we propose this bioinformatic criterion be used for design of VIP/NTM peptide mimetics for prevention and treatment of HIV disease.
T2  - Journal of Peptide Research
T1  - Design of peptide mimetics of HIV-1 gp120 for prevention and therapy of HIV disease
VL  - 62
IS  - 4
SP  - 158
EP  - 166
DO  - 10.1034/j.1399-3011.2003.00081.x
ER  - 

@article{
author = "Veljković, Nevena V. and Branch, DR and Metlaš, Radmila and Prljić, Jelena and Vlahovicek, K and Pongor, S and Veljković, Veljko",
year = "2003",
abstract = "It has been reported that the C-terminus of the second conserved region (C2) of the envelope glycoprotein gp120, encompassing peptide RSANFTDNAKTIIVQLNESVEIN (NTM), is important for infectivity and neutralization of the human immunodeficiency virus type 1 (HIV-1). It was also demonstrated that human natural anti-vasoactive intestinal peptide (VIP) antibodies reactive with this gp120 region play an important role in control of HIV disease progression. The bioinformatic analysis based on the time-frequency signal processing revealed non-obvious similarities between NTM and VIP. When tested against a battery of sera from 46 AIDS patients, these peptides, in spite of a significant difference in their primary structures, showed a similar reactivity profiles (r = 0.83). Presented results point out that similarity in the periodical pattern of some physicochemical properties in primary structures of peptides plays a significant role in determination of their immunological crossreactivity. Based on these findings, we propose this bioinformatic criterion be used for design of VIP/NTM peptide mimetics for prevention and treatment of HIV disease.",
journal = "Journal of Peptide Research",
title = "Design of peptide mimetics of HIV-1 gp120 for prevention and therapy of HIV disease",
volume = "62",
number = "4",
pages = "158-166",
doi = "10.1034/j.1399-3011.2003.00081.x"
}

Veljković, N. V., Branch, D., Metlaš, R., Prljić, J., Vlahovicek, K., Pongor, S.,& Veljković, V.. (2003). Design of peptide mimetics of HIV-1 gp120 for prevention and therapy of HIV disease. in Journal of Peptide Research, 62(4), 158-166.
https://doi.org/10.1034/j.1399-3011.2003.00081.x

Veljković NV, Branch D, Metlaš R, Prljić J, Vlahovicek K, Pongor S, Veljković V. Design of peptide mimetics of HIV-1 gp120 for prevention and therapy of HIV disease. in Journal of Peptide Research. 2003;62(4):158-166.
doi:10.1034/j.1399-3011.2003.00081.x .

Veljković, Nevena V., Branch, DR, Metlaš, Radmila, Prljić, Jelena, Vlahovicek, K, Pongor, S, Veljković, Veljko, "Design of peptide mimetics of HIV-1 gp120 for prevention and therapy of HIV disease" in Journal of Peptide Research, 62, no. 4 (2003):158-166,
https://doi.org/10.1034/j.1399-3011.2003.00081.x . .

TY  - JOUR
AU  - Veljković, Veljko
AU  - Metlaš, Radmila
AU  - Kohler, Heike
AU  - Urnovitz, HB
AU  - Prljić, Jelena
AU  - Veljković, Nevena V.
AU  - Johnson, Emmett
AU  - Muller, S
PY  - 2001
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2419
AB  - Current expansion of AIDS pandemic significantly accelerates AIDS vaccine research resulting in development and clinical testing of several AIDS vaccine candidates. At the same time, available experimental and clinical data demonstrate that current AIDS vaccine strategy is unsuccessful resulting in development of inefficient and harmful vaccines. This overview briefly summarizes reported results which point out the requirement for moratorium on the current clinical trials of HIV-1 gp120/160 vaccines and urgent need for development of a new, efficient and safe AIDS vaccine strategy. (C) 2001 Elsevier Science Ltd. All rights reserved.
T2  - Vaccine
T1  - AIDS epidemic at the beginning of the third millennium: time for a new AIDS vaccine strategy
VL  - 19
IS  - 15-16
SP  - 1855
EP  - 1862
DO  - 10.1016/S0264-410X(00)00194-8
ER  - 

@article{
author = "Veljković, Veljko and Metlaš, Radmila and Kohler, Heike and Urnovitz, HB and Prljić, Jelena and Veljković, Nevena V. and Johnson, Emmett and Muller, S",
year = "2001",
abstract = "Current expansion of AIDS pandemic significantly accelerates AIDS vaccine research resulting in development and clinical testing of several AIDS vaccine candidates. At the same time, available experimental and clinical data demonstrate that current AIDS vaccine strategy is unsuccessful resulting in development of inefficient and harmful vaccines. This overview briefly summarizes reported results which point out the requirement for moratorium on the current clinical trials of HIV-1 gp120/160 vaccines and urgent need for development of a new, efficient and safe AIDS vaccine strategy. (C) 2001 Elsevier Science Ltd. All rights reserved.",
journal = "Vaccine",
title = "AIDS epidemic at the beginning of the third millennium: time for a new AIDS vaccine strategy",
volume = "19",
number = "15-16",
pages = "1855-1862",
doi = "10.1016/S0264-410X(00)00194-8"
}

Veljković, V., Metlaš, R., Kohler, H., Urnovitz, H., Prljić, J., Veljković, N. V., Johnson, E.,& Muller, S.. (2001). AIDS epidemic at the beginning of the third millennium: time for a new AIDS vaccine strategy. in Vaccine, 19(15-16), 1855-1862.
https://doi.org/10.1016/S0264-410X(00)00194-8

Veljković V, Metlaš R, Kohler H, Urnovitz H, Prljić J, Veljković NV, Johnson E, Muller S. AIDS epidemic at the beginning of the third millennium: time for a new AIDS vaccine strategy. in Vaccine. 2001;19(15-16):1855-1862.
doi:10.1016/S0264-410X(00)00194-8 .

Veljković, Veljko, Metlaš, Radmila, Kohler, Heike, Urnovitz, HB, Prljić, Jelena, Veljković, Nevena V., Johnson, Emmett, Muller, S, "AIDS epidemic at the beginning of the third millennium: time for a new AIDS vaccine strategy" in Vaccine, 19, no. 15-16 (2001):1855-1862,
https://doi.org/10.1016/S0264-410X(00)00194-8 . .

TY  - JOUR
AU  - Veljković, Veljko
AU  - Metlaš, Radmila
AU  - Jevtović, D.
AU  - Stringer, WW
PY  - 2001
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2448
AB  - An HIV positive patient presented with pulmonary tuberculosis as her AIDS-defining diagnosis in 1993 and was effectively treated with 12 months of standard antituberculosis medications (isoniazide, rifampin, and pyrazinamide for 2 months). She received zidovudine for 6 weeks at the time of her diagnosis; however, because of patient preference, she has not received subsequent standard HIV medications (7 years). Her CD4 count at the time of diagnosis (1993) was 297/muL. Monthly passive immunotherapy was administered (fresh frozen plasma from HIV-negative blood donors with a significant titer for the anti-vasoactive intestinal peptide [VIP]/NTM antibody) from December 1993 to June 1994. Her CD4 count increased to GT 400/muL during the passive immunotherapy and has remained stable for the past 6 years. The rational for the use of anti-VIP/NTM antibodies preparations in HIV, the possible mode of action of anti-VIP/NTM antibodies, the use of Ig preparations, and the role of exercise as a natural source of anti-VIP/NTM antibodies are discussed. This case report supports the potential therapeutic use of anti-VIP antibodies for treatment of HIV disease.
T2  - Chest
T1  - The role of passive immunization in HIV-positive patients - A case report
VL  - 120
IS  - 2
SP  - 662
EP  - 666
DO  - 10.1378/chest.120.2.662
ER  - 

@article{
author = "Veljković, Veljko and Metlaš, Radmila and Jevtović, D. and Stringer, WW",
year = "2001",
abstract = "An HIV positive patient presented with pulmonary tuberculosis as her AIDS-defining diagnosis in 1993 and was effectively treated with 12 months of standard antituberculosis medications (isoniazide, rifampin, and pyrazinamide for 2 months). She received zidovudine for 6 weeks at the time of her diagnosis; however, because of patient preference, she has not received subsequent standard HIV medications (7 years). Her CD4 count at the time of diagnosis (1993) was 297/muL. Monthly passive immunotherapy was administered (fresh frozen plasma from HIV-negative blood donors with a significant titer for the anti-vasoactive intestinal peptide [VIP]/NTM antibody) from December 1993 to June 1994. Her CD4 count increased to GT 400/muL during the passive immunotherapy and has remained stable for the past 6 years. The rational for the use of anti-VIP/NTM antibodies preparations in HIV, the possible mode of action of anti-VIP/NTM antibodies, the use of Ig preparations, and the role of exercise as a natural source of anti-VIP/NTM antibodies are discussed. This case report supports the potential therapeutic use of anti-VIP antibodies for treatment of HIV disease.",
journal = "Chest",
title = "The role of passive immunization in HIV-positive patients - A case report",
volume = "120",
number = "2",
pages = "662-666",
doi = "10.1378/chest.120.2.662"
}

Veljković, V., Metlaš, R., Jevtović, D.,& Stringer, W.. (2001). The role of passive immunization in HIV-positive patients - A case report. in Chest, 120(2), 662-666.
https://doi.org/10.1378/chest.120.2.662

Veljković V, Metlaš R, Jevtović D, Stringer W. The role of passive immunization in HIV-positive patients - A case report. in Chest. 2001;120(2):662-666.
doi:10.1378/chest.120.2.662 .

Veljković, Veljko, Metlaš, Radmila, Jevtović, D., Stringer, WW, "The role of passive immunization in HIV-positive patients - A case report" in Chest, 120, no. 2 (2001):662-666,
https://doi.org/10.1378/chest.120.2.662 . .

TY  - JOUR
AU  - Prljić, Jelena
AU  - Veljković, Nevena V.
AU  - Doliana, R
AU  - Colombatti, A
AU  - Johnson, Emmett
AU  - Metlaš, Radmila
AU  - Veljković, Veljko
PY  - 1999
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2230
AB  - Because of a sequence similarity between the HIV-1 envelope glycoprotein gp120 and the variable region of human immunoglobulins, we have suggested that the use of this protein as a vaccine component could strongly influence the host immune system, making it more vulnerable to HIV, and in the long term, accelerate disease progression in asymptomatic HIV patients. Using a chimeric primer consisting of the nucleotide sequence derived from the HIV-1 env gene coding for the second conserved region of gp120, and the highly conserved sequence derived from the human immunoglobulin gene coding for the VHIII domain, we have identified in sera of AIDS patients HIV-I field isolates carrying the complete and active Chi recombinational hot spot (GCTGGTGG). We have also demonstrated in vivo recombination between the HIV-I gene coding for the central portion of the gp120 involving the V3 loop and the bacterial gene coding for the clp protease. These results strongly support and reinforce the previous contention and the serious concern that AIDS vaccine candidates carrying the HIV-1 env gene on viral and bacterial vectors, could result in the generation of new pathogens with unpredictable effects on the immune system. (C) 1999 Elsevier Science Ltd. All rights reserved.
T2  - Vaccine
T1  - Identification of an active Chi recombinational hot spot within the HIV-1 envelope gene: consequences for development of AIDS vaccines
VL  - 17
IS  - 11-12
SP  - 1462
EP  - 1467
DO  - 10.1016/S0264-410X(98)00373-9
ER  - 

@article{
author = "Prljić, Jelena and Veljković, Nevena V. and Doliana, R and Colombatti, A and Johnson, Emmett and Metlaš, Radmila and Veljković, Veljko",
year = "1999",
abstract = "Because of a sequence similarity between the HIV-1 envelope glycoprotein gp120 and the variable region of human immunoglobulins, we have suggested that the use of this protein as a vaccine component could strongly influence the host immune system, making it more vulnerable to HIV, and in the long term, accelerate disease progression in asymptomatic HIV patients. Using a chimeric primer consisting of the nucleotide sequence derived from the HIV-1 env gene coding for the second conserved region of gp120, and the highly conserved sequence derived from the human immunoglobulin gene coding for the VHIII domain, we have identified in sera of AIDS patients HIV-I field isolates carrying the complete and active Chi recombinational hot spot (GCTGGTGG). We have also demonstrated in vivo recombination between the HIV-I gene coding for the central portion of the gp120 involving the V3 loop and the bacterial gene coding for the clp protease. These results strongly support and reinforce the previous contention and the serious concern that AIDS vaccine candidates carrying the HIV-1 env gene on viral and bacterial vectors, could result in the generation of new pathogens with unpredictable effects on the immune system. (C) 1999 Elsevier Science Ltd. All rights reserved.",
journal = "Vaccine",
title = "Identification of an active Chi recombinational hot spot within the HIV-1 envelope gene: consequences for development of AIDS vaccines",
volume = "17",
number = "11-12",
pages = "1462-1467",
doi = "10.1016/S0264-410X(98)00373-9"
}

Prljić, J., Veljković, N. V., Doliana, R., Colombatti, A., Johnson, E., Metlaš, R.,& Veljković, V.. (1999). Identification of an active Chi recombinational hot spot within the HIV-1 envelope gene: consequences for development of AIDS vaccines. in Vaccine, 17(11-12), 1462-1467.
https://doi.org/10.1016/S0264-410X(98)00373-9

Prljić J, Veljković NV, Doliana R, Colombatti A, Johnson E, Metlaš R, Veljković V. Identification of an active Chi recombinational hot spot within the HIV-1 envelope gene: consequences for development of AIDS vaccines. in Vaccine. 1999;17(11-12):1462-1467.
doi:10.1016/S0264-410X(98)00373-9 .

Prljić, Jelena, Veljković, Nevena V., Doliana, R, Colombatti, A, Johnson, Emmett, Metlaš, Radmila, Veljković, Veljko, "Identification of an active Chi recombinational hot spot within the HIV-1 envelope gene: consequences for development of AIDS vaccines" in Vaccine, 17, no. 11-12 (1999):1462-1467,
https://doi.org/10.1016/S0264-410X(98)00373-9 . .

TY  - JOUR
AU  - Veljković, Veljko
AU  - Johnson, Emmett
AU  - Metlaš, Radmila
PY  - 1997
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2059
AB  - Molecular bases of possible harmful effects of AIDS vaccine candidates based on HIV-I gp120/160, that are prepared for Phase III clinical trials in developing countries, have been considered. (C) 1997 Elsevier Science Ltd.
T2  - Vaccine
T1  - Molecular basis of the inefficacy and possible harmful effects of AIDS vaccine candidates based on HIV-1 envelope glycoprotein gp120
VL  - 15
IS  - 5
SP  - 473
EP  - 474
DO  - 10.1016/S0264-410X(97)00011-X
ER  - 

@article{
author = "Veljković, Veljko and Johnson, Emmett and Metlaš, Radmila",
year = "1997",
abstract = "Molecular bases of possible harmful effects of AIDS vaccine candidates based on HIV-I gp120/160, that are prepared for Phase III clinical trials in developing countries, have been considered. (C) 1997 Elsevier Science Ltd.",
journal = "Vaccine",
title = "Molecular basis of the inefficacy and possible harmful effects of AIDS vaccine candidates based on HIV-1 envelope glycoprotein gp120",
volume = "15",
number = "5",
pages = "473-474",
doi = "10.1016/S0264-410X(97)00011-X"
}

Veljković, V., Johnson, E.,& Metlaš, R.. (1997). Molecular basis of the inefficacy and possible harmful effects of AIDS vaccine candidates based on HIV-1 envelope glycoprotein gp120. in Vaccine, 15(5), 473-474.
https://doi.org/10.1016/S0264-410X(97)00011-X

Veljković V, Johnson E, Metlaš R. Molecular basis of the inefficacy and possible harmful effects of AIDS vaccine candidates based on HIV-1 envelope glycoprotein gp120. in Vaccine. 1997;15(5):473-474.
doi:10.1016/S0264-410X(97)00011-X .

Veljković, Veljko, Johnson, Emmett, Metlaš, Radmila, "Molecular basis of the inefficacy and possible harmful effects of AIDS vaccine candidates based on HIV-1 envelope glycoprotein gp120" in Vaccine, 15, no. 5 (1997):473-474,
https://doi.org/10.1016/S0264-410X(97)00011-X . .