TY  - JOUR
AU  - Todorović, Jasna
AU  - Dinčić, Marko
AU  - Krstić, Danijela Z.
AU  - Čolović, Mirjana B.
AU  - Nešović-Ostojić, Jelena
AU  - Kovačević, Sanjin
AU  - Lopičić, Srđan
AU  - Spasić, Svetolik
AU  - Brkić, Predrag
AU  - Milovanović, Aleksandar
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10745
AB  - Background: Thyroid dysfunctions as well as sleep abnormalities are usually followed by neurological, psychiatric and/or behavioral disorders. On the other hand, changes in the brain adenosine triphosphatases (ATPases) and acetylcholinesterase (AChE) activities show significant importance in pathogenetic pathways in the evolution of numerous neuropsychiatric diseases. Methods: This study aimed to evaluate the in vivo simultaneous effects of hypothyroidism and paradoxical sleep deprivation for 72 h on synaptosomalATPases and AChE activities of whole rat brains. In order to induce hypothyroidism, 6-n-propyl-2-thiouracil was administrated in drinking water during 21 days. The modified multiple platform method was used to induce paradoxical sleep deprivation. The AChE and ATPases activities were measured using spectrophotometric methods. Results: Hypothyroidism significantly increased the activity of Na+/K+-ATPase compared to other groups, while at the same time significantly decreased AChE activity compared to the CT and SD groups. Paradoxical sleep deprivation significantly increased AChE activity compared to other groups. The simultaneous effect of hypothyroidism and sleep deprivation reduced the activity of all three enzymes (for Na+/K+-ATPase between HT/SD and HT group p < 0.0001, SD group p < 0.001,CT group p = 0.013; for ecto-ATPases between HT/SD and HT group p = 0.0034, SD group p = 0.0001, CT group p = 0.0007; for AChE between HT/SD and HT group p < 0.05, SD group p < 0.0001, CT group p < 0.0001). Conclusions: The effect of simultaneous existence of hypothyroidism and paradoxical sleep deprivation reduces the activity of the Na+/K+-ATPase, ecto-ATPases, and AChE, what is different from individual effect of hypothyroidism and paradoxical sleep deprivation itself. This knowledge could help in the choice of appropriate therapy in such condition. © 2023 Elsevier B.V.
T2  - Sleep Medicine
T1  - The simultaneous action of acute paradoxical sleep deprivation and hypothyroidism modulates synaptosomal ATPases and acetylcholinesterase activities in rat brain
VL  - 105
SP  - 14
EP  - 20
DO  - 10.1016/j.sleep.2023.03.002
ER  - 

@article{
author = "Todorović, Jasna and Dinčić, Marko and Krstić, Danijela Z. and Čolović, Mirjana B. and Nešović-Ostojić, Jelena and Kovačević, Sanjin and Lopičić, Srđan and Spasić, Svetolik and Brkić, Predrag and Milovanović, Aleksandar",
year = "2023",
abstract = "Background: Thyroid dysfunctions as well as sleep abnormalities are usually followed by neurological, psychiatric and/or behavioral disorders. On the other hand, changes in the brain adenosine triphosphatases (ATPases) and acetylcholinesterase (AChE) activities show significant importance in pathogenetic pathways in the evolution of numerous neuropsychiatric diseases. Methods: This study aimed to evaluate the in vivo simultaneous effects of hypothyroidism and paradoxical sleep deprivation for 72 h on synaptosomalATPases and AChE activities of whole rat brains. In order to induce hypothyroidism, 6-n-propyl-2-thiouracil was administrated in drinking water during 21 days. The modified multiple platform method was used to induce paradoxical sleep deprivation. The AChE and ATPases activities were measured using spectrophotometric methods. Results: Hypothyroidism significantly increased the activity of Na+/K+-ATPase compared to other groups, while at the same time significantly decreased AChE activity compared to the CT and SD groups. Paradoxical sleep deprivation significantly increased AChE activity compared to other groups. The simultaneous effect of hypothyroidism and sleep deprivation reduced the activity of all three enzymes (for Na+/K+-ATPase between HT/SD and HT group p < 0.0001, SD group p < 0.001,CT group p = 0.013; for ecto-ATPases between HT/SD and HT group p = 0.0034, SD group p = 0.0001, CT group p = 0.0007; for AChE between HT/SD and HT group p < 0.05, SD group p < 0.0001, CT group p < 0.0001). Conclusions: The effect of simultaneous existence of hypothyroidism and paradoxical sleep deprivation reduces the activity of the Na+/K+-ATPase, ecto-ATPases, and AChE, what is different from individual effect of hypothyroidism and paradoxical sleep deprivation itself. This knowledge could help in the choice of appropriate therapy in such condition. © 2023 Elsevier B.V.",
journal = "Sleep Medicine",
title = "The simultaneous action of acute paradoxical sleep deprivation and hypothyroidism modulates synaptosomal ATPases and acetylcholinesterase activities in rat brain",
volume = "105",
pages = "14-20",
doi = "10.1016/j.sleep.2023.03.002"
}

Todorović, J., Dinčić, M., Krstić, D. Z., Čolović, M. B., Nešović-Ostojić, J., Kovačević, S., Lopičić, S., Spasić, S., Brkić, P.,& Milovanović, A.. (2023). The simultaneous action of acute paradoxical sleep deprivation and hypothyroidism modulates synaptosomal ATPases and acetylcholinesterase activities in rat brain. in Sleep Medicine, 105, 14-20.
https://doi.org/10.1016/j.sleep.2023.03.002

Todorović J, Dinčić M, Krstić DZ, Čolović MB, Nešović-Ostojić J, Kovačević S, Lopičić S, Spasić S, Brkić P, Milovanović A. The simultaneous action of acute paradoxical sleep deprivation and hypothyroidism modulates synaptosomal ATPases and acetylcholinesterase activities in rat brain. in Sleep Medicine. 2023;105:14-20.
doi:10.1016/j.sleep.2023.03.002 .

Todorović, Jasna, Dinčić, Marko, Krstić, Danijela Z., Čolović, Mirjana B., Nešović-Ostojić, Jelena, Kovačević, Sanjin, Lopičić, Srđan, Spasić, Svetolik, Brkić, Predrag, Milovanović, Aleksandar, "The simultaneous action of acute paradoxical sleep deprivation and hypothyroidism modulates synaptosomal ATPases and acetylcholinesterase activities in rat brain" in Sleep Medicine, 105 (2023):14-20,
https://doi.org/10.1016/j.sleep.2023.03.002 . .

TY  - CONF
AU  - Pajić, Jelena
AU  - Jovičić, Dubravka
AU  - Rakić, Boban
AU  - Milovanović, Aleksandar
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8296
UR  - https://plus.sr.cobiss.net/opac7/bib/245691404
UR  - http://dzz.org.rs/wp-content/uploads/2013/06/Zbornik_XXIX_Simpozijum_DZZ_SCG_Srebrno_jezero_2.pdf
AB  - Jedan od uzročnika oštećenja molekula DNK dejstvom jonizujućeg zračenja je i
nastanak slobodnih radikala. Cilj istraživanja bio je utvrđivanje uticaja parametara
oksidativnog statusa na učestalost citogenetičkih biomarkera delovanja zračenja.
Istraživanje je obuhvatilo analizu uzoraka humane krvi na postojanje promena
genetičkog materijala i promena u vrednostima parametara oksidativnog statusa, u
odgovoru na delovanje tri aplikovane doze zračenja u odnosu na neozračene uzorke.
Rezultati su pokazali postojanje inter-individualne varijabilnosti u svim analiziranim
parametrima i njihovo dozno-zavisno formiranje, na svim dozama. Korelacionom
analizom parametara koji govore o sveukupnom odgovoru donora ustanovljeno je da
donori lošijeg antioksidativnog statusa pokazuju veći stepen citogenetičkih oštećenja.
Nasuprot njima, grupa donora sa boljom antioksidativnom zaštitom, pokazala je niži
odgovor na zračenje od očekivanog pri datoj dozi. Pored brojnim istraživanjima
validovanih dicentrika i mikronukleusa, i parametri oksidativnog statusa mogu, u
rutinskoj praksi radiološke zdravstvene zaštite, upotpuniti profil odgovora na
kontinuiranu ekspoziciju malim dozama, ali i reakciju na incidentalna ozračivanja.
AB  - Ionizing radiation in living cells can directly act on atomic structures, producing
damage to biomolecules (DNA). Since ionizing radiation can cause oxidative damage
and produce reactive oxygen species, the main goal of this investigation was to
establish the influence of parameters of oxidative status and oxidative damage on
chromosomal aberrations and micronuclei frequency in peripheral blood lymphocytes
from donors and to examine possible correlations.
The results showed significant inter-individual variability in all analyzed parameters, as
well as dose-dependent increases. Correlation analysis for overall response („HRR“
parameters) confirmed the t-test analysis, i.e. donors with lower values for antioxidant
status parameters had increased levels of cytogenetic damage and higher responses to
irradiation. Unlike them, the group of donors with better antioxidative protection
showed a lower response than expected. Besides well established cytogenetic
biomarkers of radiation exposure, our results indicated a promising future use for
biochemical oxidative status parameters in routine radiation protection practice, since
together they can provide a complete radiation response profile in cases of continuous
low dose exposure, as well as in a radiation emergency.
PB  - Београд : Институт за нуклеарне науке "Винча" : Друштво за заштиту од зрачења Србије и Црне Горе
C3  - 29. симпозијум ДЗЗСЦГ : зборник радова
T1  - Genotoksični markeri u limfocitima periferne krvi u odgovoru na delovanje jonizujućeg zračenja
T1  - Genotoxicity markers in human peripheral blood lymphocytes in response to ionizing radiation
SP  - 335
EP  - 342
UR  - https://hdl.handle.net/21.15107/rcub_vinar_8296
ER  - 

@conference{
author = "Pajić, Jelena and Jovičić, Dubravka and Rakić, Boban and Milovanović, Aleksandar",
year = "2017",
abstract = "Jedan od uzročnika oštećenja molekula DNK dejstvom jonizujućeg zračenja je i
nastanak slobodnih radikala. Cilj istraživanja bio je utvrđivanje uticaja parametara
oksidativnog statusa na učestalost citogenetičkih biomarkera delovanja zračenja.
Istraživanje je obuhvatilo analizu uzoraka humane krvi na postojanje promena
genetičkog materijala i promena u vrednostima parametara oksidativnog statusa, u
odgovoru na delovanje tri aplikovane doze zračenja u odnosu na neozračene uzorke.
Rezultati su pokazali postojanje inter-individualne varijabilnosti u svim analiziranim
parametrima i njihovo dozno-zavisno formiranje, na svim dozama. Korelacionom
analizom parametara koji govore o sveukupnom odgovoru donora ustanovljeno je da
donori lošijeg antioksidativnog statusa pokazuju veći stepen citogenetičkih oštećenja.
Nasuprot njima, grupa donora sa boljom antioksidativnom zaštitom, pokazala je niži
odgovor na zračenje od očekivanog pri datoj dozi. Pored brojnim istraživanjima
validovanih dicentrika i mikronukleusa, i parametri oksidativnog statusa mogu, u
rutinskoj praksi radiološke zdravstvene zaštite, upotpuniti profil odgovora na
kontinuiranu ekspoziciju malim dozama, ali i reakciju na incidentalna ozračivanja., Ionizing radiation in living cells can directly act on atomic structures, producing
damage to biomolecules (DNA). Since ionizing radiation can cause oxidative damage
and produce reactive oxygen species, the main goal of this investigation was to
establish the influence of parameters of oxidative status and oxidative damage on
chromosomal aberrations and micronuclei frequency in peripheral blood lymphocytes
from donors and to examine possible correlations.
The results showed significant inter-individual variability in all analyzed parameters, as
well as dose-dependent increases. Correlation analysis for overall response („HRR“
parameters) confirmed the t-test analysis, i.e. donors with lower values for antioxidant
status parameters had increased levels of cytogenetic damage and higher responses to
irradiation. Unlike them, the group of donors with better antioxidative protection
showed a lower response than expected. Besides well established cytogenetic
biomarkers of radiation exposure, our results indicated a promising future use for
biochemical oxidative status parameters in routine radiation protection practice, since
together they can provide a complete radiation response profile in cases of continuous
low dose exposure, as well as in a radiation emergency.",
publisher = "Београд : Институт за нуклеарне науке "Винча" : Друштво за заштиту од зрачења Србије и Црне Горе",
journal = "29. симпозијум ДЗЗСЦГ : зборник радова",
title = "Genotoksični markeri u limfocitima periferne krvi u odgovoru na delovanje jonizujućeg zračenja, Genotoxicity markers in human peripheral blood lymphocytes in response to ionizing radiation",
pages = "335-342",
url = "https://hdl.handle.net/21.15107/rcub_vinar_8296"
}

Pajić, J., Jovičić, D., Rakić, B.,& Milovanović, A.. (2017). Genotoksični markeri u limfocitima periferne krvi u odgovoru na delovanje jonizujućeg zračenja. in 29. симпозијум ДЗЗСЦГ : зборник радова
Београд : Институт за нуклеарне науке "Винча" : Друштво за заштиту од зрачења Србије и Црне Горе., 335-342.
https://hdl.handle.net/21.15107/rcub_vinar_8296

Pajić J, Jovičić D, Rakić B, Milovanović A. Genotoksični markeri u limfocitima periferne krvi u odgovoru na delovanje jonizujućeg zračenja. in 29. симпозијум ДЗЗСЦГ : зборник радова. 2017;:335-342.
https://hdl.handle.net/21.15107/rcub_vinar_8296 .

Pajić, Jelena, Jovičić, Dubravka, Rakić, Boban, Milovanović, Aleksandar, "Genotoksični markeri u limfocitima periferne krvi u odgovoru na delovanje jonizujućeg zračenja" in 29. симпозијум ДЗЗСЦГ : зборник радова (2017):335-342,
https://hdl.handle.net/21.15107/rcub_vinar_8296 .