TY  - JOUR
AU  - Alavantić, Dragan
AU  - Glišić, Sanja
AU  - Radovanovic, N
AU  - Romic, M
PY  - 1998
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6259
AB  - In this article, we describe a useful modification of the polymerase chain reaction for amplification applicable to hepatitis C virus genotyping and determination of its subtypes. The method is fast, cheap and simple for detection of any known point mutation, and could be used in every laboratory with experience in polymerase chain reaction technique. We could differentiate hepatitis C virus subtype Ib from other subtypes and 2b from 2a and other subtypes as well. We could also differentiate hepatitis C type 3 using a type-specific oligonucleotide from 3a subtype, thus covering the most common hepatitis C virus (sub)types present in the European region.
T2  - Clinical Chemistry and Laboratory Medicine
T1  - A simple PCR with different 3 ends of the third primer for detection of defined point mutations: HCV genotyping as an example
VL  - 36
IS  - 8
SP  - 587
EP  - 588
DO  - 10.1515/CCLM.1998.101
ER  - 

@article{
author = "Alavantić, Dragan and Glišić, Sanja and Radovanovic, N and Romic, M",
year = "1998",
abstract = "In this article, we describe a useful modification of the polymerase chain reaction for amplification applicable to hepatitis C virus genotyping and determination of its subtypes. The method is fast, cheap and simple for detection of any known point mutation, and could be used in every laboratory with experience in polymerase chain reaction technique. We could differentiate hepatitis C virus subtype Ib from other subtypes and 2b from 2a and other subtypes as well. We could also differentiate hepatitis C type 3 using a type-specific oligonucleotide from 3a subtype, thus covering the most common hepatitis C virus (sub)types present in the European region.",
journal = "Clinical Chemistry and Laboratory Medicine",
title = "A simple PCR with different 3 ends of the third primer for detection of defined point mutations: HCV genotyping as an example",
volume = "36",
number = "8",
pages = "587-588",
doi = "10.1515/CCLM.1998.101"
}

Alavantić, D., Glišić, S., Radovanovic, N.,& Romic, M.. (1998). A simple PCR with different 3 ends of the third primer for detection of defined point mutations: HCV genotyping as an example. in Clinical Chemistry and Laboratory Medicine, 36(8), 587-588.
https://doi.org/10.1515/CCLM.1998.101

Alavantić D, Glišić S, Radovanovic N, Romic M. A simple PCR with different 3 ends of the third primer for detection of defined point mutations: HCV genotyping as an example. in Clinical Chemistry and Laboratory Medicine. 1998;36(8):587-588.
doi:10.1515/CCLM.1998.101 .

Alavantić, Dragan, Glišić, Sanja, Radovanovic, N, Romic, M, "A simple PCR with different 3 ends of the third primer for detection of defined point mutations: HCV genotyping as an example" in Clinical Chemistry and Laboratory Medicine, 36, no. 8 (1998):587-588,
https://doi.org/10.1515/CCLM.1998.101 . .

TY  - JOUR
AU  - Alavantić, Dragan
AU  - Glišić, Sanja
AU  - Radovanovic, N
AU  - Romic, M
AU  - Medic, P
AU  - Tomovic, O
PY  - 1998
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2192
AB  - Patients receiving any kind of human blood preparations are in permanent danger of any infection including hepatitis C (HCV) infection. Testing for the presence of HCV in blood preparations is one of the steps towards safe medical treatment, One of the approaches for this testing is a detection of HCV nucleic acid. In this paper we describe a simple method for isolation of HCV RNA from blood preparations and control of HCV RNA presence in 19 intravenous and intramuscular products, manufactured in the National Blood Transfusion Institute in Belgrade. RT-PCR was performed according the rules saving RNA. Primers were located in 5 conserved region. Seven out of 19 batches of gamma-globulin, albumin, anti-tetanus and anti-rabies immunoglobulin preparations were found to be HCV RNA positive. For the time being, the PCR method is too expensive for routine HCV RNA testing of hundreds of;blood donors per day. Serological screening test of blood donors and nested PCR testing for HCV RNA. in blood preparations could be an efficient combination of tests in prevention of posttransfusion hepatitis C. (C) 1998 Elsevier Science Ltd. All rights reserved.
T2  - Transfusion Science
T1  - Hepatitis C virus RNA testing by nested PCR in blood preparations in Yugoslavia
VL  - 19
IS  - 2
SP  - 115
EP  - 117
DO  - 10.1016/S0955-3886(98)00019-8
ER  - 

@article{
author = "Alavantić, Dragan and Glišić, Sanja and Radovanovic, N and Romic, M and Medic, P and Tomovic, O",
year = "1998",
abstract = "Patients receiving any kind of human blood preparations are in permanent danger of any infection including hepatitis C (HCV) infection. Testing for the presence of HCV in blood preparations is one of the steps towards safe medical treatment, One of the approaches for this testing is a detection of HCV nucleic acid. In this paper we describe a simple method for isolation of HCV RNA from blood preparations and control of HCV RNA presence in 19 intravenous and intramuscular products, manufactured in the National Blood Transfusion Institute in Belgrade. RT-PCR was performed according the rules saving RNA. Primers were located in 5 conserved region. Seven out of 19 batches of gamma-globulin, albumin, anti-tetanus and anti-rabies immunoglobulin preparations were found to be HCV RNA positive. For the time being, the PCR method is too expensive for routine HCV RNA testing of hundreds of;blood donors per day. Serological screening test of blood donors and nested PCR testing for HCV RNA. in blood preparations could be an efficient combination of tests in prevention of posttransfusion hepatitis C. (C) 1998 Elsevier Science Ltd. All rights reserved.",
journal = "Transfusion Science",
title = "Hepatitis C virus RNA testing by nested PCR in blood preparations in Yugoslavia",
volume = "19",
number = "2",
pages = "115-117",
doi = "10.1016/S0955-3886(98)00019-8"
}

Alavantić, D., Glišić, S., Radovanovic, N., Romic, M., Medic, P.,& Tomovic, O.. (1998). Hepatitis C virus RNA testing by nested PCR in blood preparations in Yugoslavia. in Transfusion Science, 19(2), 115-117.
https://doi.org/10.1016/S0955-3886(98)00019-8

Alavantić D, Glišić S, Radovanovic N, Romic M, Medic P, Tomovic O. Hepatitis C virus RNA testing by nested PCR in blood preparations in Yugoslavia. in Transfusion Science. 1998;19(2):115-117.
doi:10.1016/S0955-3886(98)00019-8 .

Alavantić, Dragan, Glišić, Sanja, Radovanovic, N, Romic, M, Medic, P, Tomovic, O, "Hepatitis C virus RNA testing by nested PCR in blood preparations in Yugoslavia" in Transfusion Science, 19, no. 2 (1998):115-117,
https://doi.org/10.1016/S0955-3886(98)00019-8 . .

TY  - JOUR
AU  - Glišić, Sanja
AU  - Prljić, Jelena
AU  - Radovanovic, N
AU  - Alavantić, Dragan
PY  - 1997
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2076
AB  - The apolipoprotein B (apoB) signal peptide polymorphism was studied in unrelated healthy individuals. A total of 232 women and 222 men were analyzed separately. The relative frequencies of Del allele in women and men were 0.42 and 0.37, respectively. More heterozygous individuals were detected in comparison with other populations, using a modified silver staining method on polyacrylamide gel for visualization of Ins and Del alleles. There was no statistically significant difference in mean lipid levels adjusted for age, BMI, smoking habit and blood pressure between the three Ins/Del genotypes in both samples (ANOVA). Therefore, no differences were shown in the genotype frequency distribution throughout the lipid quartiles. (C) 1997 Elsevier Science B.V.
T2  - Clinica Chimica Acta
T1  - Study of apoB gene signal peptide insertion/deletion polymorphism in a healthy Serbian population: no association with serum lipid levels
VL  - 263
IS  - 1
SP  - 57
EP  - 65
DO  - 10.1016/S0009-8981(97)06556-X
ER  - 

@article{
author = "Glišić, Sanja and Prljić, Jelena and Radovanovic, N and Alavantić, Dragan",
year = "1997",
abstract = "The apolipoprotein B (apoB) signal peptide polymorphism was studied in unrelated healthy individuals. A total of 232 women and 222 men were analyzed separately. The relative frequencies of Del allele in women and men were 0.42 and 0.37, respectively. More heterozygous individuals were detected in comparison with other populations, using a modified silver staining method on polyacrylamide gel for visualization of Ins and Del alleles. There was no statistically significant difference in mean lipid levels adjusted for age, BMI, smoking habit and blood pressure between the three Ins/Del genotypes in both samples (ANOVA). Therefore, no differences were shown in the genotype frequency distribution throughout the lipid quartiles. (C) 1997 Elsevier Science B.V.",
journal = "Clinica Chimica Acta",
title = "Study of apoB gene signal peptide insertion/deletion polymorphism in a healthy Serbian population: no association with serum lipid levels",
volume = "263",
number = "1",
pages = "57-65",
doi = "10.1016/S0009-8981(97)06556-X"
}

Glišić, S., Prljić, J., Radovanovic, N.,& Alavantić, D.. (1997). Study of apoB gene signal peptide insertion/deletion polymorphism in a healthy Serbian population: no association with serum lipid levels. in Clinica Chimica Acta, 263(1), 57-65.
https://doi.org/10.1016/S0009-8981(97)06556-X

Glišić S, Prljić J, Radovanovic N, Alavantić D. Study of apoB gene signal peptide insertion/deletion polymorphism in a healthy Serbian population: no association with serum lipid levels. in Clinica Chimica Acta. 1997;263(1):57-65.
doi:10.1016/S0009-8981(97)06556-X .

Glišić, Sanja, Prljić, Jelena, Radovanovic, N, Alavantić, Dragan, "Study of apoB gene signal peptide insertion/deletion polymorphism in a healthy Serbian population: no association with serum lipid levels" in Clinica Chimica Acta, 263, no. 1 (1997):57-65,
https://doi.org/10.1016/S0009-8981(97)06556-X . .