TY  - JOUR
AU  - Pirković, Andrea
AU  - Lazić, Vesna
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Kuzman, Sanja
AU  - Antić-Stanković, Jelena
AU  - Božić, Dragana
AU  - Jovanović Krivokuća, Milica
AU  - Nedeljković, Jovan
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11219
AB  - Dry olive leaf extract (DOLE) and its active component oleuropein (OLE) were applied as reducing and stabilizing agents to prepare colloidal 20–25 nm silver nanoparticles (Ag NPs). The Ag NPs were characterized using transmission electron microscopy, X-ray diffraction analysis, and absorption spectroscopy. The cytotoxic actions of coated Ag NPs, and their inorganic and organic components, were examined against trophoblast cells and human peripheral blood lymphocytes (PBLs), Gram-positive, Gram-negative bacteria, and yeast. The genotoxic potential was evaluated in PBLs in vitro with the comet assay. Ag/DOLE and Ag/OLE induced cytotoxic effects in both types of cells after 24 h exposure when silver concentrations were 0.025–0.2 mM. However, the most pronounced cytotoxicity exhibits Ag/OLE. Both colloids also caused reduced ROS production in both cell types at 0.1 mM and 0.2 mM, while bare Ag NPs did not alter ROS levels at any of the conditions. Functionalized Ag/DOLE and Ag/OLE did not show genotoxic effects in PBLs, while bare AgNPs increased DNA damage significantly only at 0.2 mM. Regarding the antimicrobial effects, the Ag/OLE had MIC values for all evaluated microorganisms from 0.0625 to less than 0.0312 mM. Also, the antimicrobial effect of Ag/DOLE was significantly higher on Gram-negative bacteria and yeast than on Gram-positive bacteria. Obtained results indicate that Ag/OLE induced the most pronounced biological effects, beneficial for its application as an antimicrobial agent, but with potential risks from exposure to high concentrations that could induce cytotoxicity in healthy human cells.
T2  - Mutagenesis
T1  - Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes
VL  - 38
IS  - 3
SP  - 169
EP  - 181
DO  - 10.1093/mutage/gead013
ER  - 

@article{
author = "Pirković, Andrea and Lazić, Vesna and Spremo-Potparević, Biljana and Živković, Lada and Topalović, Dijana and Kuzman, Sanja and Antić-Stanković, Jelena and Božić, Dragana and Jovanović Krivokuća, Milica and Nedeljković, Jovan",
year = "2023",
abstract = "Dry olive leaf extract (DOLE) and its active component oleuropein (OLE) were applied as reducing and stabilizing agents to prepare colloidal 20–25 nm silver nanoparticles (Ag NPs). The Ag NPs were characterized using transmission electron microscopy, X-ray diffraction analysis, and absorption spectroscopy. The cytotoxic actions of coated Ag NPs, and their inorganic and organic components, were examined against trophoblast cells and human peripheral blood lymphocytes (PBLs), Gram-positive, Gram-negative bacteria, and yeast. The genotoxic potential was evaluated in PBLs in vitro with the comet assay. Ag/DOLE and Ag/OLE induced cytotoxic effects in both types of cells after 24 h exposure when silver concentrations were 0.025–0.2 mM. However, the most pronounced cytotoxicity exhibits Ag/OLE. Both colloids also caused reduced ROS production in both cell types at 0.1 mM and 0.2 mM, while bare Ag NPs did not alter ROS levels at any of the conditions. Functionalized Ag/DOLE and Ag/OLE did not show genotoxic effects in PBLs, while bare AgNPs increased DNA damage significantly only at 0.2 mM. Regarding the antimicrobial effects, the Ag/OLE had MIC values for all evaluated microorganisms from 0.0625 to less than 0.0312 mM. Also, the antimicrobial effect of Ag/DOLE was significantly higher on Gram-negative bacteria and yeast than on Gram-positive bacteria. Obtained results indicate that Ag/OLE induced the most pronounced biological effects, beneficial for its application as an antimicrobial agent, but with potential risks from exposure to high concentrations that could induce cytotoxicity in healthy human cells.",
journal = "Mutagenesis",
title = "Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes",
volume = "38",
number = "3",
pages = "169-181",
doi = "10.1093/mutage/gead013"
}

Pirković, A., Lazić, V., Spremo-Potparević, B., Živković, L., Topalović, D., Kuzman, S., Antić-Stanković, J., Božić, D., Jovanović Krivokuća, M.,& Nedeljković, J.. (2023). Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes. in Mutagenesis, 38(3), 169-181.
https://doi.org/10.1093/mutage/gead013

Pirković A, Lazić V, Spremo-Potparević B, Živković L, Topalović D, Kuzman S, Antić-Stanković J, Božić D, Jovanović Krivokuća M, Nedeljković J. Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes. in Mutagenesis. 2023;38(3):169-181.
doi:10.1093/mutage/gead013 .

Pirković, Andrea, Lazić, Vesna, Spremo-Potparević, Biljana, Živković, Lada, Topalović, Dijana, Kuzman, Sanja, Antić-Stanković, Jelena, Božić, Dragana, Jovanović Krivokuća, Milica, Nedeljković, Jovan, "Comparative analysis of Ag NPs functionalized with olive leaf extract and oleuropein and toxicity in human trophoblast cells and peripheral blood lymphocytes" in Mutagenesis, 38, no. 3 (2023):169-181,
https://doi.org/10.1093/mutage/gead013 . .

TY  - CONF
AU  - Nikšić, Valentina
AU  - Dukić, Miljana
AU  - Pirković, Andrea
AU  - Lazić, Vesna
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13039
AB  - Cilj ovog rada je modifikacija površine nanočestica TiO2 bioaktivnim ligandima, kao što je dihidrokvercetin (DHQ), formiranjem interfacijalnog kompleksa sa prenosom naelektrisanja, kako bi se postigla njegova aktivacija pod vidljivom svetlošću. Neorganskoorganski hibridni nanokompozit TiO2 /DHQ okarakterisan je infracrvenom (FTIR) i refleksionom spektroskopijom. FTIR spektri su identifikovali C=O grupe flavonoida, O-H, C-O i C-O-C grupe fenola, potvrđujući prisustvo liganada na površini nanočestica TiO2 . Kubelka-Munk transformacijom spektara difuzione refleksije vidi se pomeraj ekscitacije /DHQ prema vidljivom delu spektra. Ispitivanje citotoksičnosti MTT testom urađeno je na zdravim ljudskim MRC-5 ćelijama, kao i na ljudskim HeLa ćelijama raka grlića materice. Takođe, H2DCFDA testom ispitan je efekat TiO2 /DHQ na proizvodnju reaktivnih vrsta kiseonika u MRC-5 ćelijama.
AB  - This study aims to modify the surface of TiO2 nanoparticles with bioactive ligands, forming an interfacial charge transfer complex to achieve its activation under visible light. Dihydroquercetin (DHQ) is a catechol-type ligand with pronounced antioxidant and valuable biological performance. The inorganic-organic hybrid nanocomposite TiO2 /DHQ was characterized by Fourier transform infrared (FTIR) and Reflectance spectroscopy. FTIR spectra identified C=O stretching in flavones, O-H, C-O and C-O-C starching in phenolic compounds, demonstrating the presence of ligands on the surface of TiO2 nanoparticles. The Kubelka-Munk transformation of the diffuse reflection spectra shows a shift in the excitation of the TiO2 /DHQ towards the visible part of the spectrum. Cytotoxicity testing was performed on healthy human MRC-5 cells and human cervical cancer HeLa cells determined by MTT assay. Also, the effect of TiO2 /DHQ on the production of reactive oxygen species in MRC-5 cells was determined by the H2DCFDA assay.
PB  - Belgrade : Serbian Chemical Society
C3  - 59th Meeting of the Serbian Chemical Society : Book of Abstracts, Proceedings
T1  - Toksičnost nanočestica TiO2 modifikovanih dihidrokvarcetinom
T1  - Toxicity of TiO2 nanoparticles modified with dihydroquercetin
SP  - 146
EP  - 146
UR  - https://hdl.handle.net/21.15107/rcub_vinar_13039
ER  - 

@conference{
author = "Nikšić, Valentina and Dukić, Miljana and Pirković, Andrea and Lazić, Vesna",
year = "2023",
abstract = "Cilj ovog rada je modifikacija površine nanočestica TiO2 bioaktivnim ligandima, kao što je dihidrokvercetin (DHQ), formiranjem interfacijalnog kompleksa sa prenosom naelektrisanja, kako bi se postigla njegova aktivacija pod vidljivom svetlošću. Neorganskoorganski hibridni nanokompozit TiO2 /DHQ okarakterisan je infracrvenom (FTIR) i refleksionom spektroskopijom. FTIR spektri su identifikovali C=O grupe flavonoida, O-H, C-O i C-O-C grupe fenola, potvrđujući prisustvo liganada na površini nanočestica TiO2 . Kubelka-Munk transformacijom spektara difuzione refleksije vidi se pomeraj ekscitacije /DHQ prema vidljivom delu spektra. Ispitivanje citotoksičnosti MTT testom urađeno je na zdravim ljudskim MRC-5 ćelijama, kao i na ljudskim HeLa ćelijama raka grlića materice. Takođe, H2DCFDA testom ispitan je efekat TiO2 /DHQ na proizvodnju reaktivnih vrsta kiseonika u MRC-5 ćelijama., This study aims to modify the surface of TiO2 nanoparticles with bioactive ligands, forming an interfacial charge transfer complex to achieve its activation under visible light. Dihydroquercetin (DHQ) is a catechol-type ligand with pronounced antioxidant and valuable biological performance. The inorganic-organic hybrid nanocomposite TiO2 /DHQ was characterized by Fourier transform infrared (FTIR) and Reflectance spectroscopy. FTIR spectra identified C=O stretching in flavones, O-H, C-O and C-O-C starching in phenolic compounds, demonstrating the presence of ligands on the surface of TiO2 nanoparticles. The Kubelka-Munk transformation of the diffuse reflection spectra shows a shift in the excitation of the TiO2 /DHQ towards the visible part of the spectrum. Cytotoxicity testing was performed on healthy human MRC-5 cells and human cervical cancer HeLa cells determined by MTT assay. Also, the effect of TiO2 /DHQ on the production of reactive oxygen species in MRC-5 cells was determined by the H2DCFDA assay.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "59th Meeting of the Serbian Chemical Society : Book of Abstracts, Proceedings",
title = "Toksičnost nanočestica TiO2 modifikovanih dihidrokvarcetinom, Toxicity of TiO2 nanoparticles modified with dihydroquercetin",
pages = "146-146",
url = "https://hdl.handle.net/21.15107/rcub_vinar_13039"
}

Nikšić, V., Dukić, M., Pirković, A.,& Lazić, V.. (2023). Toksičnost nanočestica TiO2 modifikovanih dihidrokvarcetinom. in 59th Meeting of the Serbian Chemical Society : Book of Abstracts, Proceedings
Belgrade : Serbian Chemical Society., 146-146.
https://hdl.handle.net/21.15107/rcub_vinar_13039

Nikšić V, Dukić M, Pirković A, Lazić V. Toksičnost nanočestica TiO2 modifikovanih dihidrokvarcetinom. in 59th Meeting of the Serbian Chemical Society : Book of Abstracts, Proceedings. 2023;:146-146.
https://hdl.handle.net/21.15107/rcub_vinar_13039 .

Nikšić, Valentina, Dukić, Miljana, Pirković, Andrea, Lazić, Vesna, "Toksičnost nanočestica TiO2 modifikovanih dihidrokvarcetinom" in 59th Meeting of the Serbian Chemical Society : Book of Abstracts, Proceedings (2023):146-146,
https://hdl.handle.net/21.15107/rcub_vinar_13039 .

TY  - JOUR
AU  - Sredojević, Dušan
AU  - Lazić, Vesna M.
AU  - Pirković, Andrea
AU  - Periša, Jovana
AU  - Murafa, Natalija
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Topalović, Dijana
AU  - Zarubica, Aleksandra
AU  - Jovanović Krivokuća, Milica
AU  - Nedeljković, Jovan
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10445
AB  - The antibacterial performance and cytotoxic examination of in situ prepared silver nanoparticles (Ag NPs), on inorganic-organic hybrid nanopowder consisting of zirconium dioxide nanoparticles (ZrO2 NPs) and dihydroquercetin (DHQ), was performed against Gram (−) bacteria Escherichia coli and Gram (+) bacteria Staphylococcus aureus, as well as against human cervical cancer cells HeLa and healthy MRC-5 human cells. The surface modification of ZrO2 NPs, synthesized by the sol-gel method, with DHQ leads to the interfacial charge transfer (ICT) complex formation indicated by the appearance of absorption in the visible spectral range. The prepared samples were thoroughly characterized (TEM, XRD, reflection spectroscopy), and, in addition, the spectroscopic observations are supported by the density functional theory (DFT) calculations using a cluster model. The concentration- and time-dependent antibacterial tests indicated a complete reduction of bacterial species, E. coli and S. aureus, for all investigated concentrations of silver (0.10, 0.25, and 0.50 mg/mL) after 24 h of contact. On the other side, the functionalized ZrO2 NPs with DHQ, before and after deposition of Ag NPs, do not display a significant decrease in the viability of HeLa MRC-5 cells in any of the used concentrations compared to the control.
T2  - Nanomaterials
T1  - Toxicity of Silver Nanoparticles Supported by Surface-Modified Zirconium Dioxide with Dihydroquercetin
VL  - 12
IS  - 18
SP  - 3195
DO  - 10.3390/nano12183195
ER  - 

@article{
author = "Sredojević, Dušan and Lazić, Vesna M. and Pirković, Andrea and Periša, Jovana and Murafa, Natalija and Spremo-Potparević, Biljana and Živković, Lada and Topalović, Dijana and Zarubica, Aleksandra and Jovanović Krivokuća, Milica and Nedeljković, Jovan",
year = "2022",
abstract = "The antibacterial performance and cytotoxic examination of in situ prepared silver nanoparticles (Ag NPs), on inorganic-organic hybrid nanopowder consisting of zirconium dioxide nanoparticles (ZrO2 NPs) and dihydroquercetin (DHQ), was performed against Gram (−) bacteria Escherichia coli and Gram (+) bacteria Staphylococcus aureus, as well as against human cervical cancer cells HeLa and healthy MRC-5 human cells. The surface modification of ZrO2 NPs, synthesized by the sol-gel method, with DHQ leads to the interfacial charge transfer (ICT) complex formation indicated by the appearance of absorption in the visible spectral range. The prepared samples were thoroughly characterized (TEM, XRD, reflection spectroscopy), and, in addition, the spectroscopic observations are supported by the density functional theory (DFT) calculations using a cluster model. The concentration- and time-dependent antibacterial tests indicated a complete reduction of bacterial species, E. coli and S. aureus, for all investigated concentrations of silver (0.10, 0.25, and 0.50 mg/mL) after 24 h of contact. On the other side, the functionalized ZrO2 NPs with DHQ, before and after deposition of Ag NPs, do not display a significant decrease in the viability of HeLa MRC-5 cells in any of the used concentrations compared to the control.",
journal = "Nanomaterials",
title = "Toxicity of Silver Nanoparticles Supported by Surface-Modified Zirconium Dioxide with Dihydroquercetin",
volume = "12",
number = "18",
pages = "3195",
doi = "10.3390/nano12183195"
}

Sredojević, D., Lazić, V. M., Pirković, A., Periša, J., Murafa, N., Spremo-Potparević, B., Živković, L., Topalović, D., Zarubica, A., Jovanović Krivokuća, M.,& Nedeljković, J.. (2022). Toxicity of Silver Nanoparticles Supported by Surface-Modified Zirconium Dioxide with Dihydroquercetin. in Nanomaterials, 12(18), 3195.
https://doi.org/10.3390/nano12183195

Sredojević D, Lazić VM, Pirković A, Periša J, Murafa N, Spremo-Potparević B, Živković L, Topalović D, Zarubica A, Jovanović Krivokuća M, Nedeljković J. Toxicity of Silver Nanoparticles Supported by Surface-Modified Zirconium Dioxide with Dihydroquercetin. in Nanomaterials. 2022;12(18):3195.
doi:10.3390/nano12183195 .

Sredojević, Dušan, Lazić, Vesna M., Pirković, Andrea, Periša, Jovana, Murafa, Natalija, Spremo-Potparević, Biljana, Živković, Lada, Topalović, Dijana, Zarubica, Aleksandra, Jovanović Krivokuća, Milica, Nedeljković, Jovan, "Toxicity of Silver Nanoparticles Supported by Surface-Modified Zirconium Dioxide with Dihydroquercetin" in Nanomaterials, 12, no. 18 (2022):3195,
https://doi.org/10.3390/nano12183195 . .

TY  - JOUR
AU  - Lazić, Vesna M.
AU  - Pirković, Andrea
AU  - Sredojević, Dušan
AU  - Marković, Jelena P.
AU  - Papan, Jelena
AU  - Ahrenkiel, S. Phillip
AU  - Janković-Častvan, Ivona
AU  - Dekanski, Dragana
AU  - Jovanović Krivokuća, Milica
AU  - Nedeljković, Jovan
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9897
AB  - The toxicity of hybrid nanoparticles, consisting of non-toxic components, zirconium dioxide nanoparticles (ZrO2 NPs), and caffeic acid (CA), was examined against four different cell lines (HTR-8 SV/Neo, JEG-3, JAR, and HeLa). Stable aqueous ZrO2 sol, synthesized by forced hydrolysis, consists of 3–4 nm in size primary particles organized in 30–60 nm in size snowflake-like particles, as determined by transmission electron microscopy and direct light scattering measurements. The surface modification of ZrO2 NPs with CA leads to the formation of an interfacial charge transfer (ICT) complex followed by the appearance of absorption in the visible spectral range. The spectroscopic observations are complemented with the density functional theory calculations using a cluster model. The ZrO2 NPs and CA are non-toxic against four different cell lines in investigated concentration range. Also, ZrO2 NPs promote the proliferation of HTR-8 SV/Neo, JAR, and HeLa cells. On the other hand, hybrid ZrO2/CA NPs induced a significant reduction of the viability of the JEG-3 cells (39 %) for the high concentration of components (1.6 mM ZrO2 and 0.4 mM CA).
T2  - Chemico-Biological Interactions
T1  - Surface-modified ZrO2 nanoparticles with caffeic acid: Characterization and in vitro evaluation of biosafety for placental cells
VL  - 347
SP  - 109618
DO  - 10.1016/j.cbi.2021.109618
ER  - 

@article{
author = "Lazić, Vesna M. and Pirković, Andrea and Sredojević, Dušan and Marković, Jelena P. and Papan, Jelena and Ahrenkiel, S. Phillip and Janković-Častvan, Ivona and Dekanski, Dragana and Jovanović Krivokuća, Milica and Nedeljković, Jovan",
year = "2021",
abstract = "The toxicity of hybrid nanoparticles, consisting of non-toxic components, zirconium dioxide nanoparticles (ZrO2 NPs), and caffeic acid (CA), was examined against four different cell lines (HTR-8 SV/Neo, JEG-3, JAR, and HeLa). Stable aqueous ZrO2 sol, synthesized by forced hydrolysis, consists of 3–4 nm in size primary particles organized in 30–60 nm in size snowflake-like particles, as determined by transmission electron microscopy and direct light scattering measurements. The surface modification of ZrO2 NPs with CA leads to the formation of an interfacial charge transfer (ICT) complex followed by the appearance of absorption in the visible spectral range. The spectroscopic observations are complemented with the density functional theory calculations using a cluster model. The ZrO2 NPs and CA are non-toxic against four different cell lines in investigated concentration range. Also, ZrO2 NPs promote the proliferation of HTR-8 SV/Neo, JAR, and HeLa cells. On the other hand, hybrid ZrO2/CA NPs induced a significant reduction of the viability of the JEG-3 cells (39 %) for the high concentration of components (1.6 mM ZrO2 and 0.4 mM CA).",
journal = "Chemico-Biological Interactions",
title = "Surface-modified ZrO2 nanoparticles with caffeic acid: Characterization and in vitro evaluation of biosafety for placental cells",
volume = "347",
pages = "109618",
doi = "10.1016/j.cbi.2021.109618"
}

Lazić, V. M., Pirković, A., Sredojević, D., Marković, J. P., Papan, J., Ahrenkiel, S. P., Janković-Častvan, I., Dekanski, D., Jovanović Krivokuća, M.,& Nedeljković, J.. (2021). Surface-modified ZrO2 nanoparticles with caffeic acid: Characterization and in vitro evaluation of biosafety for placental cells. in Chemico-Biological Interactions, 347, 109618.
https://doi.org/10.1016/j.cbi.2021.109618

Lazić VM, Pirković A, Sredojević D, Marković JP, Papan J, Ahrenkiel SP, Janković-Častvan I, Dekanski D, Jovanović Krivokuća M, Nedeljković J. Surface-modified ZrO2 nanoparticles with caffeic acid: Characterization and in vitro evaluation of biosafety for placental cells. in Chemico-Biological Interactions. 2021;347:109618.
doi:10.1016/j.cbi.2021.109618 .

Lazić, Vesna M., Pirković, Andrea, Sredojević, Dušan, Marković, Jelena P., Papan, Jelena, Ahrenkiel, S. Phillip, Janković-Častvan, Ivona, Dekanski, Dragana, Jovanović Krivokuća, Milica, Nedeljković, Jovan, "Surface-modified ZrO2 nanoparticles with caffeic acid: Characterization and in vitro evaluation of biosafety for placental cells" in Chemico-Biological Interactions, 347 (2021):109618,
https://doi.org/10.1016/j.cbi.2021.109618 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan P.
AU  - Čabarkapa‐Pirković, Andrea
AU  - Dekanski, Dragana
AU  - Forbes‐Hernández, Tamara Yuliett
AU  - Zlatković‐Švenda, Mirjana
AU  - Perry, George
AU  - Spremo-Potparević, Biljana
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9564
AB  - Increased levels of oxidative stress and oxidative DNA damage are common features in the pathology of Alzheimer’s disease (AD) found in neurons and peripheral cells like peripheral blood lymphocytes (PBL). Natural products such as strawberry cultivar Alba are an important source of bioactive nutrients that could help in lowering both the oxidative stress and DNA damage levels. The objective was to estimate the effects of Alba extract on DNA damage in peripheral blood lymphocytes of sporadic AD (aged 60–84 years) patients, and healthy elderly (aged 69–83 years) and young (aged 21–30 years) individuals in in vitro conditions. Comet assay was used as a sensitive technique for the evaluation of PBL DNA damage levels. Reduction of basal DNA damage level in PBL was shown in the young group after the incubation with Alba extract ranging from 25 to 200 μg/ml, with 100 μg/ml being the most effective concentration. Selected Alba extract of 100 μg/ml was further used for PBL treatment of AD and healthy elderly age matched group, displaying potential to significantly attenuate DNA damage levels in both groups (p <.05). Alba extract displayed biological activity against oxidative DNA damage, suggesting that its functional ingredients may have beneficial health effects. Practical applications: The data obtained in this preliminary study displayed that strawberry Alba extract is efficient against DNA damage induced by endogenous and exogenous oxidative stress in peripheral blood lymphocytes of Alzheimer`s disease in vitro. An active area of future research of Alba cultivar should be to determine the trials in in vivo systems. Our findings also suggest that Alba cultivar’s functional ingredients potentially may have beneficial health effects in AD. © 2021 Wiley Periodicals LLC.
T2  - Journal of Food Biochemistry
T1  - Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease
VL  - 45
IS  - 4
DO  - 10.1111/jfbc.13637
ER  - 

@article{
author = "Živković, Lada and Bajić, Vladan P. and Čabarkapa‐Pirković, Andrea and Dekanski, Dragana and Forbes‐Hernández, Tamara Yuliett and Zlatković‐Švenda, Mirjana and Perry, George and Spremo-Potparević, Biljana",
year = "2021",
abstract = "Increased levels of oxidative stress and oxidative DNA damage are common features in the pathology of Alzheimer’s disease (AD) found in neurons and peripheral cells like peripheral blood lymphocytes (PBL). Natural products such as strawberry cultivar Alba are an important source of bioactive nutrients that could help in lowering both the oxidative stress and DNA damage levels. The objective was to estimate the effects of Alba extract on DNA damage in peripheral blood lymphocytes of sporadic AD (aged 60–84 years) patients, and healthy elderly (aged 69–83 years) and young (aged 21–30 years) individuals in in vitro conditions. Comet assay was used as a sensitive technique for the evaluation of PBL DNA damage levels. Reduction of basal DNA damage level in PBL was shown in the young group after the incubation with Alba extract ranging from 25 to 200 μg/ml, with 100 μg/ml being the most effective concentration. Selected Alba extract of 100 μg/ml was further used for PBL treatment of AD and healthy elderly age matched group, displaying potential to significantly attenuate DNA damage levels in both groups (p <.05). Alba extract displayed biological activity against oxidative DNA damage, suggesting that its functional ingredients may have beneficial health effects. Practical applications: The data obtained in this preliminary study displayed that strawberry Alba extract is efficient against DNA damage induced by endogenous and exogenous oxidative stress in peripheral blood lymphocytes of Alzheimer`s disease in vitro. An active area of future research of Alba cultivar should be to determine the trials in in vivo systems. Our findings also suggest that Alba cultivar’s functional ingredients potentially may have beneficial health effects in AD. © 2021 Wiley Periodicals LLC.",
journal = "Journal of Food Biochemistry",
title = "Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease",
volume = "45",
number = "4",
doi = "10.1111/jfbc.13637"
}

Živković, L., Bajić, V. P., Čabarkapa‐Pirković, A., Dekanski, D., Forbes‐Hernández, T. Y., Zlatković‐Švenda, M., Perry, G.,& Spremo-Potparević, B.. (2021). Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease. in Journal of Food Biochemistry, 45(4).
https://doi.org/10.1111/jfbc.13637

Živković L, Bajić VP, Čabarkapa‐Pirković A, Dekanski D, Forbes‐Hernández TY, Zlatković‐Švenda M, Perry G, Spremo-Potparević B. Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease. in Journal of Food Biochemistry. 2021;45(4).
doi:10.1111/jfbc.13637 .

Živković, Lada, Bajić, Vladan P., Čabarkapa‐Pirković, Andrea, Dekanski, Dragana, Forbes‐Hernández, Tamara Yuliett, Zlatković‐Švenda, Mirjana, Perry, George, Spremo-Potparević, Biljana, "Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease" in Journal of Food Biochemistry, 45, no. 4 (2021),
https://doi.org/10.1111/jfbc.13637 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan P.
AU  - Dekanski, Dragana
AU  - Čabarkapa-Pirković, Andrea
AU  - Giampieri, Francesca
AU  - Gasparrini, Massimiliano
AU  - Mazzoni, Luca
AU  - Spremo-Potparević, Biljana
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S027869151830334X
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7819
AB  - Manuka honey has been widely researched regarding its biological properties, in particular its antimicrobial and antioxidant capacities. We tested the genotoxic and genoprotective properties of Manuka honey, ranging from 25–1000 μg/mL, by performing an in vitro comet assay after exposure to human whole blood. No genotoxic effect on whole blood cells was observed within the tested concentration range (p = 0.154). Then, the antigenotoxic potency of Manuka honey against oxidative DNA damage to whole blood cells was assessed. Prior to Manuka honey treatment a modest decrease of H2O2-induced DNA damage was detected in cells, with no statistical significance (p = 0.087). Post-treatment, Manuka honey displayed a stronger potential to attenuate damaged cells at all tested concentrations, with a statistical significant difference (p < 0.001), where concentrations of 25 and 100 μg/mL were most efficient. Manuka honey exhibited a marked potential to protect DNA of whole blood cells from oxidative damage induced by hydrogen peroxide in vitro.
T2  - Food and Chemical Toxicology
T1  - Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro
VL  - 119
SP  - 61
EP  - 65
DO  - 10.1016/j.fct.2018.05.034
ER  - 

@article{
author = "Živković, Lada and Bajić, Vladan P. and Dekanski, Dragana and Čabarkapa-Pirković, Andrea and Giampieri, Francesca and Gasparrini, Massimiliano and Mazzoni, Luca and Spremo-Potparević, Biljana",
year = "2018",
abstract = "Manuka honey has been widely researched regarding its biological properties, in particular its antimicrobial and antioxidant capacities. We tested the genotoxic and genoprotective properties of Manuka honey, ranging from 25–1000 μg/mL, by performing an in vitro comet assay after exposure to human whole blood. No genotoxic effect on whole blood cells was observed within the tested concentration range (p = 0.154). Then, the antigenotoxic potency of Manuka honey against oxidative DNA damage to whole blood cells was assessed. Prior to Manuka honey treatment a modest decrease of H2O2-induced DNA damage was detected in cells, with no statistical significance (p = 0.087). Post-treatment, Manuka honey displayed a stronger potential to attenuate damaged cells at all tested concentrations, with a statistical significant difference (p < 0.001), where concentrations of 25 and 100 μg/mL were most efficient. Manuka honey exhibited a marked potential to protect DNA of whole blood cells from oxidative damage induced by hydrogen peroxide in vitro.",
journal = "Food and Chemical Toxicology",
title = "Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro",
volume = "119",
pages = "61-65",
doi = "10.1016/j.fct.2018.05.034"
}

Živković, L., Bajić, V. P., Dekanski, D., Čabarkapa-Pirković, A., Giampieri, F., Gasparrini, M., Mazzoni, L.,& Spremo-Potparević, B.. (2018). Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro. in Food and Chemical Toxicology, 119, 61-65.
https://doi.org/10.1016/j.fct.2018.05.034

Živković L, Bajić VP, Dekanski D, Čabarkapa-Pirković A, Giampieri F, Gasparrini M, Mazzoni L, Spremo-Potparević B. Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro. in Food and Chemical Toxicology. 2018;119:61-65.
doi:10.1016/j.fct.2018.05.034 .

Živković, Lada, Bajić, Vladan P., Dekanski, Dragana, Čabarkapa-Pirković, Andrea, Giampieri, Francesca, Gasparrini, Massimiliano, Mazzoni, Luca, Spremo-Potparević, Biljana, "Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro" in Food and Chemical Toxicology, 119 (2018):61-65,
https://doi.org/10.1016/j.fct.2018.05.034 . .

TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Čabarkapa, Andrea
AU  - Kotur-Stevuljevic, Jelena
AU  - Isenović, Esma R.
AU  - Sredojević, Dušan
AU  - Vukoje, Ivana D.
AU  - Lazić, Vesna M.
AU  - Ahrenkiel, Scott Phillip
AU  - Nedeljković, Jovan
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1607
AB  - The antigenotoxic and antioxidative properties of surface-modified TiO2 nanoparticles (NPs) with ascorbic acid (AA) were compared with those of constituents (free AA and bare TiO2 NPs). Colloids consisting of the TiO2 NPs with anatase crystal structure were prepared by acidic hydrolysis of TiCl4. The synthesized TiO2 NPs were characterized using transmission electron microscopy and X-ray diffraction analysis. The charge transfer (CT) complex formation between surface Ti atoms and AA is indicated by immediate appearance of red color. Composition and stability constants of CT complex were determined using Jobs method and Banesi-Hildebrand analysis, respectively. The surface structure of CT complex was determined from infra-red spectra of free and bound AA to the surface Ti atoms. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). The antigenotoxic potential of CT complex was evaluated in leukocytes of whole blood cells in vitro by comet assay method. For evaluation of antioxidant properties, total antioxidant status (TAS) and total oxidant status (TOS) were determined in human serum pool in vitro. The presented results indicate that bare TiO2 NPs have more pronounced antigenotoxic effects in comparison with either surface-modified TiO2 NPs with AA or free AA. No significant differences between the antigenotoxic and antioxidative properties of free and bound AA on the TiO2 NPs were noticed in the investigated concentration range. It seems that surface-modified TiO2 NPs with AA and/or similar compounds can be used to maintain its beneficial activities. (C) 2017 Elsevier B.V. All rights reserved.
T2  - Colloids and Surfaces. B: Biointerfaces
T1  - Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro
VL  - 155
SP  - 323
EP  - 331
DO  - 10.1016/j.colsurfb.2017.04.032
ER  - 

@article{
author = "Bajić, Vladan P. and Spremo-Potparević, Biljana and Živković, Lada and Čabarkapa, Andrea and Kotur-Stevuljevic, Jelena and Isenović, Esma R. and Sredojević, Dušan and Vukoje, Ivana D. and Lazić, Vesna M. and Ahrenkiel, Scott Phillip and Nedeljković, Jovan",
year = "2017",
abstract = "The antigenotoxic and antioxidative properties of surface-modified TiO2 nanoparticles (NPs) with ascorbic acid (AA) were compared with those of constituents (free AA and bare TiO2 NPs). Colloids consisting of the TiO2 NPs with anatase crystal structure were prepared by acidic hydrolysis of TiCl4. The synthesized TiO2 NPs were characterized using transmission electron microscopy and X-ray diffraction analysis. The charge transfer (CT) complex formation between surface Ti atoms and AA is indicated by immediate appearance of red color. Composition and stability constants of CT complex were determined using Jobs method and Banesi-Hildebrand analysis, respectively. The surface structure of CT complex was determined from infra-red spectra of free and bound AA to the surface Ti atoms. The experimental data were supported with quantum chemical calculations based on density functional theory (DFT). The antigenotoxic potential of CT complex was evaluated in leukocytes of whole blood cells in vitro by comet assay method. For evaluation of antioxidant properties, total antioxidant status (TAS) and total oxidant status (TOS) were determined in human serum pool in vitro. The presented results indicate that bare TiO2 NPs have more pronounced antigenotoxic effects in comparison with either surface-modified TiO2 NPs with AA or free AA. No significant differences between the antigenotoxic and antioxidative properties of free and bound AA on the TiO2 NPs were noticed in the investigated concentration range. It seems that surface-modified TiO2 NPs with AA and/or similar compounds can be used to maintain its beneficial activities. (C) 2017 Elsevier B.V. All rights reserved.",
journal = "Colloids and Surfaces. B: Biointerfaces",
title = "Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro",
volume = "155",
pages = "323-331",
doi = "10.1016/j.colsurfb.2017.04.032"
}

Bajić, V. P., Spremo-Potparević, B., Živković, L., Čabarkapa, A., Kotur-Stevuljevic, J., Isenović, E. R., Sredojević, D., Vukoje, I. D., Lazić, V. M., Ahrenkiel, S. P.,& Nedeljković, J.. (2017). Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro. in Colloids and Surfaces. B: Biointerfaces, 155, 323-331.
https://doi.org/10.1016/j.colsurfb.2017.04.032

Bajić VP, Spremo-Potparević B, Živković L, Čabarkapa A, Kotur-Stevuljevic J, Isenović ER, Sredojević D, Vukoje ID, Lazić VM, Ahrenkiel SP, Nedeljković J. Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro. in Colloids and Surfaces. B: Biointerfaces. 2017;155:323-331.
doi:10.1016/j.colsurfb.2017.04.032 .

Bajić, Vladan P., Spremo-Potparević, Biljana, Živković, Lada, Čabarkapa, Andrea, Kotur-Stevuljevic, Jelena, Isenović, Esma R., Sredojević, Dušan, Vukoje, Ivana D., Lazić, Vesna M., Ahrenkiel, Scott Phillip, Nedeljković, Jovan, "Surface-modified TiO2 nanoparticles with ascorbic acid: Antioxidant properties and efficiency against DNA damage in vitro" in Colloids and Surfaces. B: Biointerfaces, 155 (2017):323-331,
https://doi.org/10.1016/j.colsurfb.2017.04.032 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Borozan, Sunčica Z.
AU  - Čabarkapa, Andrea
AU  - Topalović, Dijana
AU  - Ciptasari, Ummi
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1458
AB  - The ability of Agaricus blazei mushroom in its dried and powdered mycelial form was evaluated for its antigenotoxic properties for the first time. Antigenotoxic effects in human peripheral blood cells against H2O2-induced DNA damage were examined in pretreatment and posttreatment protocol by comet assay. The results showed better antigenotoxic properties of Agaricus blazei on the interventional level, respectively, after treatment. Agaricus blazei in concentration of 250 mu g/mL after treatment was most efficient in regard to its action against DNA damage. The evaluation of repair kinetics showed decrease in H2O2 induced DNA damage 15min after the application of A. blazei, reaching the maximum potency after 30 min. Analysis of antioxidant properties of Agaricus blazei revealed strong center dot OH scavenging properties and moderate reducing power, while its DPPH scavenging ability was weak. In regard to our findings, we can conclude that our preliminary results demonstrated antigenotoxic properties of Agaricus blazei and its strong center dot OH scavenging ability. Mechanisms underlying its properties should be further evaluated in in vivo studies.
T2  - Oxidative Medicine and Cellular Longevity
T1  - Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells
DO  - 10.1155/2017/8759764
ER  - 

@article{
author = "Živković, Lada and Borozan, Sunčica Z. and Čabarkapa, Andrea and Topalović, Dijana and Ciptasari, Ummi and Bajić, Vladan P. and Spremo-Potparević, Biljana",
year = "2017",
abstract = "The ability of Agaricus blazei mushroom in its dried and powdered mycelial form was evaluated for its antigenotoxic properties for the first time. Antigenotoxic effects in human peripheral blood cells against H2O2-induced DNA damage were examined in pretreatment and posttreatment protocol by comet assay. The results showed better antigenotoxic properties of Agaricus blazei on the interventional level, respectively, after treatment. Agaricus blazei in concentration of 250 mu g/mL after treatment was most efficient in regard to its action against DNA damage. The evaluation of repair kinetics showed decrease in H2O2 induced DNA damage 15min after the application of A. blazei, reaching the maximum potency after 30 min. Analysis of antioxidant properties of Agaricus blazei revealed strong center dot OH scavenging properties and moderate reducing power, while its DPPH scavenging ability was weak. In regard to our findings, we can conclude that our preliminary results demonstrated antigenotoxic properties of Agaricus blazei and its strong center dot OH scavenging ability. Mechanisms underlying its properties should be further evaluated in in vivo studies.",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells",
doi = "10.1155/2017/8759764"
}

Živković, L., Borozan, S. Z., Čabarkapa, A., Topalović, D., Ciptasari, U., Bajić, V. P.,& Spremo-Potparević, B.. (2017). Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells. in Oxidative Medicine and Cellular Longevity.
https://doi.org/10.1155/2017/8759764

Živković L, Borozan SZ, Čabarkapa A, Topalović D, Ciptasari U, Bajić VP, Spremo-Potparević B. Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells. in Oxidative Medicine and Cellular Longevity. 2017;.
doi:10.1155/2017/8759764 .

Živković, Lada, Borozan, Sunčica Z., Čabarkapa, Andrea, Topalović, Dijana, Ciptasari, Ummi, Bajić, Vladan P., Spremo-Potparević, Biljana, "Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells" in Oxidative Medicine and Cellular Longevity (2017),
https://doi.org/10.1155/2017/8759764 . .

TY  - JOUR
AU  - Čabarkapa, Andrea
AU  - Dekanski, Dragana
AU  - Živković, Lada
AU  - Milanovic-Cabarkapa, Mirjana
AU  - Bajić, Vladan P.
AU  - Topalović, Dijana
AU  - Giampieri, Francesca
AU  - Gasparrini, Massimiliano
AU  - Battino, Maurizio
AU  - Spremo-Potparević, Biljana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1679
AB  - The CaNa(2)EDTA chelation therapy is often practiced with antioxidant supplementation. Dry olive leaf extract (DOLE) is natural product with antioxidant and DNA protective properties. The effects of DOLE on the levels of DNA damage were investigated ex vivo in peripheral blood lymphocytes (PBLs) of 19 workers occupationally exposed to lead (Pb), before and after CaNa(2)EDTA chelation therapy. POLE demonstrated pronounced radical scavenging activity in concentrations GT = 1 mg/mL, and showed no genotoxicity per se, in concentrations 0.125-1 mg/mL. The level of DNA damage in PBLs of workers before chelation therapy was elevated (24.21 +/- 14.26) compared to controls (6.0 +/- 3.37). The incubation of PBLs before chelation therapy with selected concentration of DOLE lead to a severe increase of DNA damage (64.03 +/- 20.96), exhibiting prooxidant rather than antioxidant effect. After the five-day CaNa2EDTA chelation regimen, DNA damage in PBLs of workers decreased (8.26 +/- 4.62) significantly compared to baseline. Treatment of PBLs with DOLE after chelation, again produced high level of damage (41.82 +/- 23.17) and the acute prooxidant effects of DOLE remained, but, DNA damage was less severe than before chelation. The DOLE exhibits prooxidant effect in presence of Pb in lymphocytes of exposed workers, and its effect is less pronounced following the removal of Pb after standard chelation therapy. (C) 2016 Elsevier Ltd. All rights reserved.
T2  - Food and Chemical Toxicology
T1  - Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy
VL  - 106
SP  - 616
EP  - 623
DO  - 10.1016/j.fct.2016.12.023
ER  - 

@article{
author = "Čabarkapa, Andrea and Dekanski, Dragana and Živković, Lada and Milanovic-Cabarkapa, Mirjana and Bajić, Vladan P. and Topalović, Dijana and Giampieri, Francesca and Gasparrini, Massimiliano and Battino, Maurizio and Spremo-Potparević, Biljana",
year = "2017",
abstract = "The CaNa(2)EDTA chelation therapy is often practiced with antioxidant supplementation. Dry olive leaf extract (DOLE) is natural product with antioxidant and DNA protective properties. The effects of DOLE on the levels of DNA damage were investigated ex vivo in peripheral blood lymphocytes (PBLs) of 19 workers occupationally exposed to lead (Pb), before and after CaNa(2)EDTA chelation therapy. POLE demonstrated pronounced radical scavenging activity in concentrations GT = 1 mg/mL, and showed no genotoxicity per se, in concentrations 0.125-1 mg/mL. The level of DNA damage in PBLs of workers before chelation therapy was elevated (24.21 +/- 14.26) compared to controls (6.0 +/- 3.37). The incubation of PBLs before chelation therapy with selected concentration of DOLE lead to a severe increase of DNA damage (64.03 +/- 20.96), exhibiting prooxidant rather than antioxidant effect. After the five-day CaNa2EDTA chelation regimen, DNA damage in PBLs of workers decreased (8.26 +/- 4.62) significantly compared to baseline. Treatment of PBLs with DOLE after chelation, again produced high level of damage (41.82 +/- 23.17) and the acute prooxidant effects of DOLE remained, but, DNA damage was less severe than before chelation. The DOLE exhibits prooxidant effect in presence of Pb in lymphocytes of exposed workers, and its effect is less pronounced following the removal of Pb after standard chelation therapy. (C) 2016 Elsevier Ltd. All rights reserved.",
journal = "Food and Chemical Toxicology",
title = "Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy",
volume = "106",
pages = "616-623",
doi = "10.1016/j.fct.2016.12.023"
}

Čabarkapa, A., Dekanski, D., Živković, L., Milanovic-Cabarkapa, M., Bajić, V. P., Topalović, D., Giampieri, F., Gasparrini, M., Battino, M.,& Spremo-Potparević, B.. (2017). Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy. in Food and Chemical Toxicology, 106, 616-623.
https://doi.org/10.1016/j.fct.2016.12.023

Čabarkapa A, Dekanski D, Živković L, Milanovic-Cabarkapa M, Bajić VP, Topalović D, Giampieri F, Gasparrini M, Battino M, Spremo-Potparević B. Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy. in Food and Chemical Toxicology. 2017;106:616-623.
doi:10.1016/j.fct.2016.12.023 .

Čabarkapa, Andrea, Dekanski, Dragana, Živković, Lada, Milanovic-Cabarkapa, Mirjana, Bajić, Vladan P., Topalović, Dijana, Giampieri, Francesca, Gasparrini, Massimiliano, Battino, Maurizio, Spremo-Potparević, Biljana, "Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy" in Food and Chemical Toxicology, 106 (2017):616-623,
https://doi.org/10.1016/j.fct.2016.12.023 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Čabarkapa, Andrea
AU  - Marčetić, Mirjana
AU  - Kovačević, Nada
AU  - Bajić, Vladan P.
AU  - Jovičić, Snežana
AU  - Spremo-Potparević, Biljana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1121
AB  - The essential oils of genus Seseli are known for their beneficial biological activities and could present novel targets in the development of safe and effective preparations of plant products. The objective was to test the essential oils of different parts of Seseli rigidum from two natural habitats for potential genotoxic and antigenotoxic activities against H2O2-induced DNA damage in human whole blood cells in vitro, by the comet assay. The essential oil analysis showed a high falcarinol content in oil from the root, while oils of the fruit and aerial parts contained a-pinene as the main compound. Genotoxicity was not detected at any of the concentrations of the essential oils from the three parts of the plant from localities I and II. Although the antioxidant activity (established by the FRAP and DPPH tests) of the investigated oils was low, all oils demonstrated a strong antigenotoxic effect against H2O2-induced damage post-treatment, when the oils were applied after the oxidant. Based on the lack of pretreatment activity and the post-treatment reduction in DNA damage, the antigenotoxic effect of S. rigidum essential oils was probably based on the stimulation of DNA repair mechanisms. Environmental conditions did not affect the antigenotoxic properties of the oils. In conclusion, our results revealed the antigenotoxic properties of S. rigidum essential oils and appropriate and safe doses with beneficial effects under the described conditions.
T2  - Archives of Biological Sciences
T1  - Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae)
VL  - 68
IS  - 1
SP  - 135
EP  - 144
DO  - 10.2298/ABS150512135Z
ER  - 

@article{
author = "Živković, Lada and Čabarkapa, Andrea and Marčetić, Mirjana and Kovačević, Nada and Bajić, Vladan P. and Jovičić, Snežana and Spremo-Potparević, Biljana",
year = "2016",
abstract = "The essential oils of genus Seseli are known for their beneficial biological activities and could present novel targets in the development of safe and effective preparations of plant products. The objective was to test the essential oils of different parts of Seseli rigidum from two natural habitats for potential genotoxic and antigenotoxic activities against H2O2-induced DNA damage in human whole blood cells in vitro, by the comet assay. The essential oil analysis showed a high falcarinol content in oil from the root, while oils of the fruit and aerial parts contained a-pinene as the main compound. Genotoxicity was not detected at any of the concentrations of the essential oils from the three parts of the plant from localities I and II. Although the antioxidant activity (established by the FRAP and DPPH tests) of the investigated oils was low, all oils demonstrated a strong antigenotoxic effect against H2O2-induced damage post-treatment, when the oils were applied after the oxidant. Based on the lack of pretreatment activity and the post-treatment reduction in DNA damage, the antigenotoxic effect of S. rigidum essential oils was probably based on the stimulation of DNA repair mechanisms. Environmental conditions did not affect the antigenotoxic properties of the oils. In conclusion, our results revealed the antigenotoxic properties of S. rigidum essential oils and appropriate and safe doses with beneficial effects under the described conditions.",
journal = "Archives of Biological Sciences",
title = "Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae)",
volume = "68",
number = "1",
pages = "135-144",
doi = "10.2298/ABS150512135Z"
}

Živković, L., Čabarkapa, A., Marčetić, M., Kovačević, N., Bajić, V. P., Jovičić, S.,& Spremo-Potparević, B.. (2016). Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae). in Archives of Biological Sciences, 68(1), 135-144.
https://doi.org/10.2298/ABS150512135Z

Živković L, Čabarkapa A, Marčetić M, Kovačević N, Bajić VP, Jovičić S, Spremo-Potparević B. Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae). in Archives of Biological Sciences. 2016;68(1):135-144.
doi:10.2298/ABS150512135Z .

Živković, Lada, Čabarkapa, Andrea, Marčetić, Mirjana, Kovačević, Nada, Bajić, Vladan P., Jovičić, Snežana, Spremo-Potparević, Biljana, "Evaluation of Genotoxic and Antigenotoxic Properties of Essential Oils of Seseli Rigidum Waldst. and Kit. (Apiaceae)" in Archives of Biological Sciences, 68, no. 1 (2016):135-144,
https://doi.org/10.2298/ABS150512135Z . .

TY  - JOUR
AU  - Čabarkapa, Andrea
AU  - Živković, Lada
AU  - Borozan, Sunčica Z.
AU  - Zlatković-Svenda, Mirjana
AU  - Dekanski, Dragana
AU  - Jancic, Ivan
AU  - Radak-Perović, Marija
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1282
AB  - The effects of co-administration of dry olive leaf extract (DOLE) with standard methotrexate (MTX) therapy on the parameters of cell damage and inflammation in patients with early and long-term rheumatoid arthritis (RA) were evaluated at baseline, 3 and 6weeks. Patients were assigned to groups: the early phase RA group on MTX monotherapy (E MTX), and the two RA groups that received co-treatment with DOLE and MTX: early (E MTX+DOLE) and long-term phase patients (L-t MTX+ DOLE). Baseline values indicated increased parameters of cell damage and disruption of redox balance in all groups. After three weeks the E MTX+DOLE group maintained high catalase activity, exhibited decrease of lipid peroxidation and protein damage indicatorsthiols and nitrites, while levels of DNA damage and pro-inflammatory interleukin-6 were significantly reduced. In E MTX group catalase activity remained unaltered while significant lipid peroxidation and DNA damage reductions were seen only after six weeks. L-t MTX+DOLE group showed only modest alterations of cell damage parameters during six weeks. Combined administration of DOLE with MTX contributes to faster reduction of cell damage, restores oxidative balance and improves interleukin-6 suppression during high disease activity in early phase RA, but not in long term patients. Copyright (c) 2016 John Wiley and Sons, Ltd.
T2  - Phytotherapy Research
T1  - Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study
VL  - 30
IS  - 10
SP  - 1615
EP  - 1623
DO  - 10.1002/ptr.5662
ER  - 

@article{
author = "Čabarkapa, Andrea and Živković, Lada and Borozan, Sunčica Z. and Zlatković-Svenda, Mirjana and Dekanski, Dragana and Jancic, Ivan and Radak-Perović, Marija and Bajić, Vladan P. and Spremo-Potparević, Biljana",
year = "2016",
abstract = "The effects of co-administration of dry olive leaf extract (DOLE) with standard methotrexate (MTX) therapy on the parameters of cell damage and inflammation in patients with early and long-term rheumatoid arthritis (RA) were evaluated at baseline, 3 and 6weeks. Patients were assigned to groups: the early phase RA group on MTX monotherapy (E MTX), and the two RA groups that received co-treatment with DOLE and MTX: early (E MTX+DOLE) and long-term phase patients (L-t MTX+ DOLE). Baseline values indicated increased parameters of cell damage and disruption of redox balance in all groups. After three weeks the E MTX+DOLE group maintained high catalase activity, exhibited decrease of lipid peroxidation and protein damage indicatorsthiols and nitrites, while levels of DNA damage and pro-inflammatory interleukin-6 were significantly reduced. In E MTX group catalase activity remained unaltered while significant lipid peroxidation and DNA damage reductions were seen only after six weeks. L-t MTX+DOLE group showed only modest alterations of cell damage parameters during six weeks. Combined administration of DOLE with MTX contributes to faster reduction of cell damage, restores oxidative balance and improves interleukin-6 suppression during high disease activity in early phase RA, but not in long term patients. Copyright (c) 2016 John Wiley and Sons, Ltd.",
journal = "Phytotherapy Research",
title = "Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study",
volume = "30",
number = "10",
pages = "1615-1623",
doi = "10.1002/ptr.5662"
}

Čabarkapa, A., Živković, L., Borozan, S. Z., Zlatković-Svenda, M., Dekanski, D., Jancic, I., Radak-Perović, M., Bajić, V. P.,& Spremo-Potparević, B.. (2016). Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study. in Phytotherapy Research, 30(10), 1615-1623.
https://doi.org/10.1002/ptr.5662

Čabarkapa A, Živković L, Borozan SZ, Zlatković-Svenda M, Dekanski D, Jancic I, Radak-Perović M, Bajić VP, Spremo-Potparević B. Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study. in Phytotherapy Research. 2016;30(10):1615-1623.
doi:10.1002/ptr.5662 .

Čabarkapa, Andrea, Živković, Lada, Borozan, Sunčica Z., Zlatković-Svenda, Mirjana, Dekanski, Dragana, Jancic, Ivan, Radak-Perović, Marija, Bajić, Vladan P., Spremo-Potparević, Biljana, "Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study" in Phytotherapy Research, 30, no. 10 (2016):1615-1623,
https://doi.org/10.1002/ptr.5662 . .

TY  - JOUR
AU  - Vasiljević, Jovana
AU  - Živković, Lada
AU  - Čabarkapa, Andrea
AU  - Bajić, Vladan P.
AU  - Đelić, Ninoslav
AU  - Spremo-Potparević, Biljana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1311
AB  - Context Cordyceps sinensis (C sinensis) is a well-known, traditional, Chinese medicinal mushroom, valued for its beneficial properties for human health. C sinensis has been reported to have immunomodulatory, anticancer, antiaging, antioxidant and anti-inflammatory activity. Despite potential medicinal benefits, no previously published reports are available about the genotoxicity or antigenotoxicity of C sinensis, as detected by comet assay. Objective The objective of the study was to evaluate both the genotoxic and antigenotoxic potential of an extract of C sinensis (CS extract) in human peripheral blood cells. Design The research team designed a pilot study. Setting The study was conducted at the Center for Biological Research, University of Belgrade, in Belgrade, Serbia. Participants Participants were 6 healthy individuals (2 males and 4 females), between the ages of 20 and 45 y, recruited on a voluntary basis, who provided heparinized, peripheral blood samples. Intervention Four concentrations of the CS extract125 mu g/mL, 250 mu g/mL, 500 mu g/mL, and 1000 mu g/mL-were used in the treatment of tested blood cells from the blood samples. Three independent procedures were performed: (1) a genotoxicity assessment, (2) an antigenotoxicity assessment for pretreatment of human cells with the CS extract prior to their exposure to hydrogen peroxide (H2O2) (ie, an evaluation of the benefits of the CS extract as a preventive agent); and (3) posttreatment of human cells with the CS extract after their exposure to H2O2 (ie, an evaluation of the benefits of the CS extract as an interventional agent). Outcome Measures Cells were graded by eye inspection into 5 classes, depending on the extent of DNA damage, representing: (1) class A-undamaged cells with no tail ( LT 5% damaged DNA); (2) class B-low-level damage (5%-20%); (3) class C-medium-level damage (20%-40%); (4) class D-high-level damage (40%-95%), and (5) class E-total destruction ( GT 95%). Results The CS extract proved to be nongenotoxic because no induced DNA damage was detected at all tested concentrations. For the antigenotoxicity assessment of the pretreatment with the CS extract, only the 1000-mu g/mL concentration showed a significant decrease in the number of cells exhibiting H2O2-induced DNA damage. For the posttreatment, the CS extract exhibited antigenotoxic potential by attenuating H2O2-induced DNA damage at all concentrations tested. The evaluation of repair kinetics showed a decrease in DNA-damaged cells 15 min after the application of the CS extract, reaching a maximum potency after 45 min. Conclusions The results indicated that C sinensis can be used as a postapplicative agent that counteracts the effect of oxidative stress. The resulting reduction in DNA damage might be related to its scavenging properties and stimulation of DNA repair.
T2  - Alternative Therapies in Health and Medicine
T1  - Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay
VL  - 22
SP  - 24
EP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_vinar_1311
ER  - 

@article{
author = "Vasiljević, Jovana and Živković, Lada and Čabarkapa, Andrea and Bajić, Vladan P. and Đelić, Ninoslav and Spremo-Potparević, Biljana",
year = "2016",
abstract = "Context Cordyceps sinensis (C sinensis) is a well-known, traditional, Chinese medicinal mushroom, valued for its beneficial properties for human health. C sinensis has been reported to have immunomodulatory, anticancer, antiaging, antioxidant and anti-inflammatory activity. Despite potential medicinal benefits, no previously published reports are available about the genotoxicity or antigenotoxicity of C sinensis, as detected by comet assay. Objective The objective of the study was to evaluate both the genotoxic and antigenotoxic potential of an extract of C sinensis (CS extract) in human peripheral blood cells. Design The research team designed a pilot study. Setting The study was conducted at the Center for Biological Research, University of Belgrade, in Belgrade, Serbia. Participants Participants were 6 healthy individuals (2 males and 4 females), between the ages of 20 and 45 y, recruited on a voluntary basis, who provided heparinized, peripheral blood samples. Intervention Four concentrations of the CS extract125 mu g/mL, 250 mu g/mL, 500 mu g/mL, and 1000 mu g/mL-were used in the treatment of tested blood cells from the blood samples. Three independent procedures were performed: (1) a genotoxicity assessment, (2) an antigenotoxicity assessment for pretreatment of human cells with the CS extract prior to their exposure to hydrogen peroxide (H2O2) (ie, an evaluation of the benefits of the CS extract as a preventive agent); and (3) posttreatment of human cells with the CS extract after their exposure to H2O2 (ie, an evaluation of the benefits of the CS extract as an interventional agent). Outcome Measures Cells were graded by eye inspection into 5 classes, depending on the extent of DNA damage, representing: (1) class A-undamaged cells with no tail ( LT 5% damaged DNA); (2) class B-low-level damage (5%-20%); (3) class C-medium-level damage (20%-40%); (4) class D-high-level damage (40%-95%), and (5) class E-total destruction ( GT 95%). Results The CS extract proved to be nongenotoxic because no induced DNA damage was detected at all tested concentrations. For the antigenotoxicity assessment of the pretreatment with the CS extract, only the 1000-mu g/mL concentration showed a significant decrease in the number of cells exhibiting H2O2-induced DNA damage. For the posttreatment, the CS extract exhibited antigenotoxic potential by attenuating H2O2-induced DNA damage at all concentrations tested. The evaluation of repair kinetics showed a decrease in DNA-damaged cells 15 min after the application of the CS extract, reaching a maximum potency after 45 min. Conclusions The results indicated that C sinensis can be used as a postapplicative agent that counteracts the effect of oxidative stress. The resulting reduction in DNA damage might be related to its scavenging properties and stimulation of DNA repair.",
journal = "Alternative Therapies in Health and Medicine",
title = "Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay",
volume = "22",
pages = "24-31",
url = "https://hdl.handle.net/21.15107/rcub_vinar_1311"
}

Vasiljević, J., Živković, L., Čabarkapa, A., Bajić, V. P., Đelić, N.,& Spremo-Potparević, B.. (2016). Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay. in Alternative Therapies in Health and Medicine, 22, 24-31.
https://hdl.handle.net/21.15107/rcub_vinar_1311

Vasiljević J, Živković L, Čabarkapa A, Bajić VP, Đelić N, Spremo-Potparević B. Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay. in Alternative Therapies in Health and Medicine. 2016;22:24-31.
https://hdl.handle.net/21.15107/rcub_vinar_1311 .

Vasiljević, Jovana, Živković, Lada, Čabarkapa, Andrea, Bajić, Vladan P., Đelić, Ninoslav, Spremo-Potparević, Biljana, "Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay" in Alternative Therapies in Health and Medicine, 22 (2016):24-31,
https://hdl.handle.net/21.15107/rcub_vinar_1311 .

TY  - JOUR
AU  - Bajić, Vladan P.
AU  - Mandušić, Vesna
AU  - Stefanova, Elka
AU  - Božović, Ana M.
AU  - Davidović, Radoslav S.
AU  - Živković, Lada
AU  - Čabarkapa, Andrea
AU  - Spremo-Potparević, Biljana
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/314
AB  - X-chromosome instability has been a long established feature in Alzheimers disease ( AD). Premature centromere division and aneuploidy of the X-chromosome has been found in peripheral blood lymphocytes and neuronal tissue in female AD patients. Interestingly, only one chromosome of the X pair has been affected. These results raised a question, Is the X-chromosome inactivation pattern altered in peripheral blood lymphocytes ofwomen affected by AD? To address this question, we analyzed the methylation status of androgen receptor promoter which may show us any deviation from the 50 : 50% X inactivation status in peripheral blood lymphocytes ofwomen with AD. Our results showed skewed inactivation patterns ( GT 90%). These findings suggest that an epigenetic alteration on the inactivation centers of the X-chromosome (or skewing) relates not only to aging, by might be a novel property that could account for the higher incidence of AD in women.
T2  - Journal of Alzheimers Disease
T1  - Skewed X-Chromosome Inactivation in Women Affected by Alzheimers Disease
VL  - 43
IS  - 4
SP  - 1251
EP  - 1259
DO  - 10.3233/JAD-141674
ER  - 

@article{
author = "Bajić, Vladan P. and Mandušić, Vesna and Stefanova, Elka and Božović, Ana M. and Davidović, Radoslav S. and Živković, Lada and Čabarkapa, Andrea and Spremo-Potparević, Biljana",
year = "2015",
abstract = "X-chromosome instability has been a long established feature in Alzheimers disease ( AD). Premature centromere division and aneuploidy of the X-chromosome has been found in peripheral blood lymphocytes and neuronal tissue in female AD patients. Interestingly, only one chromosome of the X pair has been affected. These results raised a question, Is the X-chromosome inactivation pattern altered in peripheral blood lymphocytes ofwomen affected by AD? To address this question, we analyzed the methylation status of androgen receptor promoter which may show us any deviation from the 50 : 50% X inactivation status in peripheral blood lymphocytes ofwomen with AD. Our results showed skewed inactivation patterns ( GT 90%). These findings suggest that an epigenetic alteration on the inactivation centers of the X-chromosome (or skewing) relates not only to aging, by might be a novel property that could account for the higher incidence of AD in women.",
journal = "Journal of Alzheimers Disease",
title = "Skewed X-Chromosome Inactivation in Women Affected by Alzheimers Disease",
volume = "43",
number = "4",
pages = "1251-1259",
doi = "10.3233/JAD-141674"
}

Bajić, V. P., Mandušić, V., Stefanova, E., Božović, A. M., Davidović, R. S., Živković, L., Čabarkapa, A.,& Spremo-Potparević, B.. (2015). Skewed X-Chromosome Inactivation in Women Affected by Alzheimers Disease. in Journal of Alzheimers Disease, 43(4), 1251-1259.
https://doi.org/10.3233/JAD-141674

Bajić VP, Mandušić V, Stefanova E, Božović AM, Davidović RS, Živković L, Čabarkapa A, Spremo-Potparević B. Skewed X-Chromosome Inactivation in Women Affected by Alzheimers Disease. in Journal of Alzheimers Disease. 2015;43(4):1251-1259.
doi:10.3233/JAD-141674 .

Bajić, Vladan P., Mandušić, Vesna, Stefanova, Elka, Božović, Ana M., Davidović, Radoslav S., Živković, Lada, Čabarkapa, Andrea, Spremo-Potparević, Biljana, "Skewed X-Chromosome Inactivation in Women Affected by Alzheimers Disease" in Journal of Alzheimers Disease, 43, no. 4 (2015):1251-1259,
https://doi.org/10.3233/JAD-141674 . .

TY  - JOUR
AU  - Čabarkapa-Pirković, Andrea 
AU  - Borozan, Sunčica Z.
AU  - Živković, Lada
AU  - Stojanovic, Srdan D.
AU  - Milanović-Čabarkapa, Mirjana
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/873
AB  - Lead induced oxidative cellular damage and long-term persistence of associated adverse effects increases risk of late-onset diseases. CaNa(2)EDTA chelation is known to remove contaminating metals and to reduce free radical production. The objective was to investigate the impact of chelation therapy on modulation of lead induced cellular damage, restoration of altered enzyme activities and lipid homeostasis in peripheral blood of workers exposed to lead, by comparing the selected biomarkers obtained prior and after five-day CaNa(2)EDTA chelation intervention. The group of smelting factory workers diagnosed with lead intoxication and current lead exposure 5.8 +/- 1.2 years were administered five-day CaNa(2)EDTA chelation. Elevated baseline activity of antioxidant enzymes Cu, Zn-SOD and CAT as well as depleted thiols and increased protein degradation products-carbonyl groups and nitrites, pointing to Pb induced oxidative damage, were restored toward normal values following the treatment. Lead showed inhibitor potency on both RBC AChE and BChE in exposed workers, and chelation re-established the activity of BChE, while RBC AChE remained unaffected. Also, genotoxic effect of lead detected in peripheral blood lymphocytes was significantly decreased after therapy, exhibiting 18.9% DNA damage reduction. Administration of chelation reversed the depressed activity of serum PON 1 and significantly decreased lipid peroxidation detected by the post-chelation reduction of MDA levels. Lactate dehydrogenase LDF1-5 isoenzymes levels showed evident but no significant trend of restoring toward normal control values following chelation. CaNa(2)EDTA chelation ameliorates the alterations linked with Pb mediated oxidative stress, indicating possible benefits in reducing health risks associated with increased oxidative damage in lead exposed populations. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
PB  - Elsevier
T2  - Chemico-Biological Interactions
T1  - CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study
VL  - 242
SP  - 171
EP  - 178
DO  - 10.1016/j.cbi.2015.10.002
ER  - 

@article{
author = "Čabarkapa-Pirković, Andrea  and Borozan, Sunčica Z. and Živković, Lada and Stojanovic, Srdan D. and Milanović-Čabarkapa, Mirjana and Bajić, Vladan P. and Spremo-Potparević, Biljana",
year = "2015",
abstract = "Lead induced oxidative cellular damage and long-term persistence of associated adverse effects increases risk of late-onset diseases. CaNa(2)EDTA chelation is known to remove contaminating metals and to reduce free radical production. The objective was to investigate the impact of chelation therapy on modulation of lead induced cellular damage, restoration of altered enzyme activities and lipid homeostasis in peripheral blood of workers exposed to lead, by comparing the selected biomarkers obtained prior and after five-day CaNa(2)EDTA chelation intervention. The group of smelting factory workers diagnosed with lead intoxication and current lead exposure 5.8 +/- 1.2 years were administered five-day CaNa(2)EDTA chelation. Elevated baseline activity of antioxidant enzymes Cu, Zn-SOD and CAT as well as depleted thiols and increased protein degradation products-carbonyl groups and nitrites, pointing to Pb induced oxidative damage, were restored toward normal values following the treatment. Lead showed inhibitor potency on both RBC AChE and BChE in exposed workers, and chelation re-established the activity of BChE, while RBC AChE remained unaffected. Also, genotoxic effect of lead detected in peripheral blood lymphocytes was significantly decreased after therapy, exhibiting 18.9% DNA damage reduction. Administration of chelation reversed the depressed activity of serum PON 1 and significantly decreased lipid peroxidation detected by the post-chelation reduction of MDA levels. Lactate dehydrogenase LDF1-5 isoenzymes levels showed evident but no significant trend of restoring toward normal control values following chelation. CaNa(2)EDTA chelation ameliorates the alterations linked with Pb mediated oxidative stress, indicating possible benefits in reducing health risks associated with increased oxidative damage in lead exposed populations. (C) 2015 Elsevier Ireland Ltd. All rights reserved.",
publisher = "Elsevier",
journal = "Chemico-Biological Interactions",
title = "CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study",
volume = "242",
pages = "171-178",
doi = "10.1016/j.cbi.2015.10.002"
}

Čabarkapa-Pirković, A., Borozan, S. Z., Živković, L., Stojanovic, S. D., Milanović-Čabarkapa, M., Bajić, V. P.,& Spremo-Potparević, B.. (2015). CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study. in Chemico-Biological Interactions
Elsevier., 242, 171-178.
https://doi.org/10.1016/j.cbi.2015.10.002

Čabarkapa-Pirković A, Borozan SZ, Živković L, Stojanovic SD, Milanović-Čabarkapa M, Bajić VP, Spremo-Potparević B. CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study. in Chemico-Biological Interactions. 2015;242:171-178.
doi:10.1016/j.cbi.2015.10.002 .

Čabarkapa-Pirković, Andrea , Borozan, Sunčica Z., Živković, Lada, Stojanovic, Srdan D., Milanović-Čabarkapa, Mirjana, Bajić, Vladan P., Spremo-Potparević, Biljana, "CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study" in Chemico-Biological Interactions, 242 (2015):171-178,
https://doi.org/10.1016/j.cbi.2015.10.002 . .

TY  - JOUR
AU  - Topalović Žukovec, Dijana
AU  - Živković, Lada
AU  - Čabarkapa, Andrea
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan P.
AU  - Dekanski, Dragana
AU  - Spremo-Potparević, Biljana
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/432
AB  - The thyroid hormones change the rate of basal metabolism, modulating the consumption of oxygen and causing production of reactive oxygen species, which leads to the development of oxidative stress and DNA strand breaks. Olive (Olea europaea L.) leaf contains many potentially bioactive compounds, making it one of the most potent natural antioxidants. The objective of this study was to evaluate the genotoxicity of L-thyroxine and to investigate antioxidative and antigenotoxic potential of the standardized oleuropein-rich dry olive leaf extract (DOLE) against hydrogen peroxide and L-thyroxine-induced DNA damage in human peripheral blood leukocytes by using the comet assay. Various concentrations of the extract were tested with both DNA damage inducers, under two different experimental conditions, pretreatment and posttreatment. Results indicate that L-thyroxine exhibited genotoxic effect and that DOLE displayed protective effect against thyroxine-induced genotoxicity. The number of cells with DNA damage, was significantly reduced, in both pretreated and posttreated samples (P LT 0.05). Comparing the beneficial effect of all tested concentrations of DOLE, in both experimental protocols, it appears that extract was more effective in reducing DNA damage in the pretreatment, exhibiting protective role against L-thyroxine effect. This feature of DOLE can be explained by its capacity to act as potent free radical scavenger.
T2  - Oxidative Medicine and Cellular Longevity
T1  - Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro
DO  - 10.1155/2015/762192
ER  - 

@article{
author = "Topalović Žukovec, Dijana and Živković, Lada and Čabarkapa, Andrea and Đelić, Ninoslav and Bajić, Vladan P. and Dekanski, Dragana and Spremo-Potparević, Biljana",
year = "2015",
abstract = "The thyroid hormones change the rate of basal metabolism, modulating the consumption of oxygen and causing production of reactive oxygen species, which leads to the development of oxidative stress and DNA strand breaks. Olive (Olea europaea L.) leaf contains many potentially bioactive compounds, making it one of the most potent natural antioxidants. The objective of this study was to evaluate the genotoxicity of L-thyroxine and to investigate antioxidative and antigenotoxic potential of the standardized oleuropein-rich dry olive leaf extract (DOLE) against hydrogen peroxide and L-thyroxine-induced DNA damage in human peripheral blood leukocytes by using the comet assay. Various concentrations of the extract were tested with both DNA damage inducers, under two different experimental conditions, pretreatment and posttreatment. Results indicate that L-thyroxine exhibited genotoxic effect and that DOLE displayed protective effect against thyroxine-induced genotoxicity. The number of cells with DNA damage, was significantly reduced, in both pretreated and posttreated samples (P LT 0.05). Comparing the beneficial effect of all tested concentrations of DOLE, in both experimental protocols, it appears that extract was more effective in reducing DNA damage in the pretreatment, exhibiting protective role against L-thyroxine effect. This feature of DOLE can be explained by its capacity to act as potent free radical scavenger.",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro",
doi = "10.1155/2015/762192"
}

Topalović Žukovec, D., Živković, L., Čabarkapa, A., Đelić, N., Bajić, V. P., Dekanski, D.,& Spremo-Potparević, B.. (2015). Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro. in Oxidative Medicine and Cellular Longevity.
https://doi.org/10.1155/2015/762192

Topalović Žukovec D, Živković L, Čabarkapa A, Đelić N, Bajić VP, Dekanski D, Spremo-Potparević B. Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro. in Oxidative Medicine and Cellular Longevity. 2015;.
doi:10.1155/2015/762192 .

Topalović Žukovec, Dijana, Živković, Lada, Čabarkapa, Andrea, Đelić, Ninoslav, Bajić, Vladan P., Dekanski, Dragana, Spremo-Potparević, Biljana, "Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro" in Oxidative Medicine and Cellular Longevity (2015),
https://doi.org/10.1155/2015/762192 . .

TY  - JOUR
AU  - Čabarkapa, Andrea
AU  - Borozan, Sunčica Z.
AU  - Živković, Lada
AU  - Milanovic-Cabarkapa, Mirjana
AU  - Stojanovic, Srdan
AU  - Bajić, Vladan P.
AU  - Spremo-Potparević, Biljana
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/703
AB  - The aim of this study was to determine oxidative alterations leading to cellular dysfunctions in Pb-exposed subjects by evaluating damage to all major classes of biomolecules in the cell, lipid peroxidation, protein and DNA damage and determine relationships between parameters of Pb toxicity and specific biomarkers of oxidative damage.Analysis was conducted of smelter workers with high blood Pb and urine aminolevulinic acid levels and slightly elevated values of coproporphyrin and erythrocyte protoporphyrin IX. Significant decreases of thiol groups and increases in carbonyl groups as protein degradation end products, and of nitrite were detected. Elevated rates of lipid peroxidation and rises in the activities of the antioxidant enzymes Cu-Zn superoxide dismutase and catalase were also observed. Both enzymes showed positive correlations with the blood lead levels and urine coproporphyrin, while thiol groups correlated negatively with the same indices. The genotoxic potential of lead was manifested through an increased number of DNA-damaged cells. Increased activities of serum lactate dehydrogenase isoenzymes indicated cellular damage in the lungs, kidneys, and liver. These lead-induced impairments should be taken into consideration in the assessment of Pb-related health hazards.
T2  - Toxicological and Environmental Chemistry
T1  - Implications of oxidative stress in occupational exposure to lead on a cellular level
VL  - 97
IS  - 6
SP  - 799
EP  - 813
DO  - 10.1080/02772248.2015.1060973
ER  - 

@article{
author = "Čabarkapa, Andrea and Borozan, Sunčica Z. and Živković, Lada and Milanovic-Cabarkapa, Mirjana and Stojanovic, Srdan and Bajić, Vladan P. and Spremo-Potparević, Biljana",
year = "2015",
abstract = "The aim of this study was to determine oxidative alterations leading to cellular dysfunctions in Pb-exposed subjects by evaluating damage to all major classes of biomolecules in the cell, lipid peroxidation, protein and DNA damage and determine relationships between parameters of Pb toxicity and specific biomarkers of oxidative damage.Analysis was conducted of smelter workers with high blood Pb and urine aminolevulinic acid levels and slightly elevated values of coproporphyrin and erythrocyte protoporphyrin IX. Significant decreases of thiol groups and increases in carbonyl groups as protein degradation end products, and of nitrite were detected. Elevated rates of lipid peroxidation and rises in the activities of the antioxidant enzymes Cu-Zn superoxide dismutase and catalase were also observed. Both enzymes showed positive correlations with the blood lead levels and urine coproporphyrin, while thiol groups correlated negatively with the same indices. The genotoxic potential of lead was manifested through an increased number of DNA-damaged cells. Increased activities of serum lactate dehydrogenase isoenzymes indicated cellular damage in the lungs, kidneys, and liver. These lead-induced impairments should be taken into consideration in the assessment of Pb-related health hazards.",
journal = "Toxicological and Environmental Chemistry",
title = "Implications of oxidative stress in occupational exposure to lead on a cellular level",
volume = "97",
number = "6",
pages = "799-813",
doi = "10.1080/02772248.2015.1060973"
}

Čabarkapa, A., Borozan, S. Z., Živković, L., Milanovic-Cabarkapa, M., Stojanovic, S., Bajić, V. P.,& Spremo-Potparević, B.. (2015). Implications of oxidative stress in occupational exposure to lead on a cellular level. in Toxicological and Environmental Chemistry, 97(6), 799-813.
https://doi.org/10.1080/02772248.2015.1060973

Čabarkapa A, Borozan SZ, Živković L, Milanovic-Cabarkapa M, Stojanovic S, Bajić VP, Spremo-Potparević B. Implications of oxidative stress in occupational exposure to lead on a cellular level. in Toxicological and Environmental Chemistry. 2015;97(6):799-813.
doi:10.1080/02772248.2015.1060973 .

Čabarkapa, Andrea, Borozan, Sunčica Z., Živković, Lada, Milanovic-Cabarkapa, Mirjana, Stojanovic, Srdan, Bajić, Vladan P., Spremo-Potparević, Biljana, "Implications of oxidative stress in occupational exposure to lead on a cellular level" in Toxicological and Environmental Chemistry, 97, no. 6 (2015):799-813,
https://doi.org/10.1080/02772248.2015.1060973 . .