TY  - JOUR
AU  - Đorđević, Ana
AU  - Živković, Maja
AU  - Bošković, Maja
AU  - Dekleva, Milica
AU  - Stanković, Goran
AU  - Stanković, Aleksandra
AU  - Đurić, Tamara
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10617
AB  - Galectin-3 is encoded by LGALS-3, located in a unique haplotype block in Caucasians. According to the Tagger server, rs4040064, rs11628437, and rs7159490 cover 82% (r2 > 0.8) of the genetic variance of this HapBlock. Our aims were to examine the association of their haplotypes with first myocardial infarction (MI), changes in left ventricular echocardiographic parameters over time, and impact on plasma galectin-3 and LGALS-3 mRNA in peripheral blood mononuclear cells, both 6 months post-MI. The study group consisted of 546 MI patients and 323 controls. Gene expression was assessed in 92 patients and plasma galectin-3 in 189 patients. Rs4040064, rs11628437, rs7159490, and LGALS-3 mRNA expression were detected using TaqMan® technology. Plasma galectin-3 concentrations were determined by the ELISA method. We found that the TGC haplotype could have a protective effect against MI (adjusted OR 0.19 [0.05–0.72], p = 0.015) and that the GAC haplotype had significantly higher galectin-3 concentrations (48.3 [37.3–59.4] ng/mL vs. 18.9 [14.5–23.4] ng/mL, p < 0.0001), both in males and compared to the referent haplotype GGC. Higher plasma Gal-3 was also associated with higher NYHA class and systolic dysfunction. Our results suggest that variants tagging LGALS-3 HapBlock could reflect plasma Gal-3 levels 6 months post-MI and may have a potential protective effect against MI in men. Further replication, validation, and functional studies are needed.
T2  - Genes
T1  - Variants Tagging LGALS-3 Haplotype Block in Association with First Myocardial Infarction and Plasma Galectin-3 Six Months after the Acute Event
VL  - 14
IS  - 1
SP  - 109
DO  - 10.3390/genes14010109
ER  - 

@article{
author = "Đorđević, Ana and Živković, Maja and Bošković, Maja and Dekleva, Milica and Stanković, Goran and Stanković, Aleksandra and Đurić, Tamara",
year = "2023",
abstract = "Galectin-3 is encoded by LGALS-3, located in a unique haplotype block in Caucasians. According to the Tagger server, rs4040064, rs11628437, and rs7159490 cover 82% (r2 > 0.8) of the genetic variance of this HapBlock. Our aims were to examine the association of their haplotypes with first myocardial infarction (MI), changes in left ventricular echocardiographic parameters over time, and impact on plasma galectin-3 and LGALS-3 mRNA in peripheral blood mononuclear cells, both 6 months post-MI. The study group consisted of 546 MI patients and 323 controls. Gene expression was assessed in 92 patients and plasma galectin-3 in 189 patients. Rs4040064, rs11628437, rs7159490, and LGALS-3 mRNA expression were detected using TaqMan® technology. Plasma galectin-3 concentrations were determined by the ELISA method. We found that the TGC haplotype could have a protective effect against MI (adjusted OR 0.19 [0.05–0.72], p = 0.015) and that the GAC haplotype had significantly higher galectin-3 concentrations (48.3 [37.3–59.4] ng/mL vs. 18.9 [14.5–23.4] ng/mL, p < 0.0001), both in males and compared to the referent haplotype GGC. Higher plasma Gal-3 was also associated with higher NYHA class and systolic dysfunction. Our results suggest that variants tagging LGALS-3 HapBlock could reflect plasma Gal-3 levels 6 months post-MI and may have a potential protective effect against MI in men. Further replication, validation, and functional studies are needed.",
journal = "Genes",
title = "Variants Tagging LGALS-3 Haplotype Block in Association with First Myocardial Infarction and Plasma Galectin-3 Six Months after the Acute Event",
volume = "14",
number = "1",
pages = "109",
doi = "10.3390/genes14010109"
}

Đorđević, A., Živković, M., Bošković, M., Dekleva, M., Stanković, G., Stanković, A.,& Đurić, T.. (2023). Variants Tagging LGALS-3 Haplotype Block in Association with First Myocardial Infarction and Plasma Galectin-3 Six Months after the Acute Event. in Genes, 14(1), 109.
https://doi.org/10.3390/genes14010109

Đorđević A, Živković M, Bošković M, Dekleva M, Stanković G, Stanković A, Đurić T. Variants Tagging LGALS-3 Haplotype Block in Association with First Myocardial Infarction and Plasma Galectin-3 Six Months after the Acute Event. in Genes. 2023;14(1):109.
doi:10.3390/genes14010109 .

Đorđević, Ana, Živković, Maja, Bošković, Maja, Dekleva, Milica, Stanković, Goran, Stanković, Aleksandra, Đurić, Tamara, "Variants Tagging LGALS-3 Haplotype Block in Association with First Myocardial Infarction and Plasma Galectin-3 Six Months after the Acute Event" in Genes, 14, no. 1 (2023):109,
https://doi.org/10.3390/genes14010109 . .

TY  - CONF
AU  - Dekleva, Milica
AU  - Martinović, M.
AU  - Stevanović, Angelina
AU  - Živković, Maja
AU  - Đorđević, Ana
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12854
AB  - Background: Right ventricular (RV) dysfunction is recognized as a cardinal prognostic marker in heart failure (HF) patients. Myocardial infarction (MI) is often followed by unrecognized RV dysfunction, which can be associated with worse outcome. It is recently shown that the ratio between TAPSE and PASP (RV/PA) may depict cardiopulmonary hemodynamics better than the two parameters alone. Aim: To evaluate the interactions between left ventricular (LV) and RV function in early phase of MI and to assess the prognostic significance of RV/PA coupling in patients with first MI during 5 years follow up. Methods: The prospective study included 144 patients with the first MI treated with the primary percutaneous coronary intervention (p PCI) who underwent Doppler echocardiography within 2±1 days of MI. LV function analysis included: LV ejection fraction (EF), ratio between early diastolic velocity and tissue annular velocity (E/e) and global longitudinal strain (GLS). RV function and RV-PA interaction was expressed as ratio between TAPSE and PASP. During the five-year follow-up, major cardiovascular events and especially hospitalization for HF were analyzed. Results: Progressive RV/PA uncoupling was associated with higher degree of LV impairment and dysfunction (EF p<0.001, E/e p=0.002, GLS p<0.001) and severity of mitral regurgitation (p=0.013). Lower baseline RV/PA coupling significantly reflects the frequency of hospitalizations for HF in the population of patients with first MI during five-year follow-up (0.62 v.s.0.51, p=0.021). After multivariate adjustment RV/PA remained an independent predictor of all major cardiac events (MACE) after five years (OR 14.0 [1.5–130.8], p=0.019). Conclusion: A lower baseline RV-PA coupling, reflecting a higher degree of LV-induced pulmonary hypertension and secondary RV-dysfunction, is associated with decline of LV function in early phase of MI, and is independently associated with worse prognosis after five years. The value of RV-PA ratio as an prognstic marker warrants further investigation.
C3  - European Heart Journal
T1  - Right ventricular-pulmonary artery coupling in the early stages of infarction could optimize patient risk stratification over five years
VL  - 44
IS  - Supplement 2
SP  - ehad655.067
DO  - 10.1093/eurheartj/ehad655.067
ER  - 

@conference{
author = "Dekleva, Milica and Martinović, M. and Stevanović, Angelina and Živković, Maja and Đorđević, Ana and Đurić, Tamara and Stanković, Aleksandra",
year = "2023",
abstract = "Background: Right ventricular (RV) dysfunction is recognized as a cardinal prognostic marker in heart failure (HF) patients. Myocardial infarction (MI) is often followed by unrecognized RV dysfunction, which can be associated with worse outcome. It is recently shown that the ratio between TAPSE and PASP (RV/PA) may depict cardiopulmonary hemodynamics better than the two parameters alone. Aim: To evaluate the interactions between left ventricular (LV) and RV function in early phase of MI and to assess the prognostic significance of RV/PA coupling in patients with first MI during 5 years follow up. Methods: The prospective study included 144 patients with the first MI treated with the primary percutaneous coronary intervention (p PCI) who underwent Doppler echocardiography within 2±1 days of MI. LV function analysis included: LV ejection fraction (EF), ratio between early diastolic velocity and tissue annular velocity (E/e) and global longitudinal strain (GLS). RV function and RV-PA interaction was expressed as ratio between TAPSE and PASP. During the five-year follow-up, major cardiovascular events and especially hospitalization for HF were analyzed. Results: Progressive RV/PA uncoupling was associated with higher degree of LV impairment and dysfunction (EF p<0.001, E/e p=0.002, GLS p<0.001) and severity of mitral regurgitation (p=0.013). Lower baseline RV/PA coupling significantly reflects the frequency of hospitalizations for HF in the population of patients with first MI during five-year follow-up (0.62 v.s.0.51, p=0.021). After multivariate adjustment RV/PA remained an independent predictor of all major cardiac events (MACE) after five years (OR 14.0 [1.5–130.8], p=0.019). Conclusion: A lower baseline RV-PA coupling, reflecting a higher degree of LV-induced pulmonary hypertension and secondary RV-dysfunction, is associated with decline of LV function in early phase of MI, and is independently associated with worse prognosis after five years. The value of RV-PA ratio as an prognstic marker warrants further investigation.",
journal = "European Heart Journal",
title = "Right ventricular-pulmonary artery coupling in the early stages of infarction could optimize patient risk stratification over five years",
volume = "44",
number = "Supplement 2",
pages = "ehad655.067",
doi = "10.1093/eurheartj/ehad655.067"
}

Dekleva, M., Martinović, M., Stevanović, A., Živković, M., Đorđević, A., Đurić, T.,& Stanković, A.. (2023). Right ventricular-pulmonary artery coupling in the early stages of infarction could optimize patient risk stratification over five years. in European Heart Journal, 44(Supplement 2), ehad655.067.
https://doi.org/10.1093/eurheartj/ehad655.067

Dekleva M, Martinović M, Stevanović A, Živković M, Đorđević A, Đurić T, Stanković A. Right ventricular-pulmonary artery coupling in the early stages of infarction could optimize patient risk stratification over five years. in European Heart Journal. 2023;44(Supplement 2):ehad655.067.
doi:10.1093/eurheartj/ehad655.067 .

Dekleva, Milica, Martinović, M., Stevanović, Angelina, Živković, Maja, Đorđević, Ana, Đurić, Tamara, Stanković, Aleksandra, "Right ventricular-pulmonary artery coupling in the early stages of infarction could optimize patient risk stratification over five years" in European Heart Journal, 44, no. Supplement 2 (2023):ehad655.067,
https://doi.org/10.1093/eurheartj/ehad655.067 . .

TY  - CONF
AU  - Kuveljić, Jovana
AU  - Životić, Ivan
AU  - Dekleva, Milica
AU  - Živković, Maja
AU  - Đurić, Tamara
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12648
AB  - Introduction: Myocardial infarction (MI) and consequential ischemia with cardiomyocyte loss are followed by left ventricular (LV) remodeling. LV remodeling is crucial process for cardiac function preservation, although when prolonged it can become maladaptive and lead to impaired systolic function and further cardiovascular complications. Echocardiographic parameters are used as a measure of LV structure and function. ADAM17 (a disintegrin and metalloprotease domain) and CDKN1A (cyclindependent kinase inhibitor 1A) have shown regulating role in DNA repair, inflammation, remodeling and fibrosis. The aim of this preliminary study was to investigate the potential effect of CDKN1 and ADAM17 mRNA in post MI heart remodeling. Methods: Sixty four patients with the first MI were prospectively followed-up 6 months after MI. Change (Δ) of echocardiographic parameters within 6 months was calculated as a difference between the value at 6-month follow-up and value at admission. Relative gene expression was detected using the TaqMan® technology. Statistical analyses were done by Statistica 8 software. Results: We have observed correlation between CDKN1A mRNA expression and change of LV enddiastolic diameter (ΔLVEDD, R=0.3, p=0.01) and LV end-systolic diameter (ΔLVESD, R=0.3, p=0.02), but not with LV ejection fraction and stroke volume. ADAM17 expression was not in correlation with analyzed parameters of LV remodeling. However, CDKN1A and ADAM17 mRNA expression in PBMC six months after MI were positively correlated (R=0.6, p<0.001). Conclusion: Preliminary resultssuggest that CDKN1 has a role in post MI LV remodeling, correlating with changes in echocardiographic parameters of LV structure. The validation on a larger sample size is required.
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts
T1  - Correlations of CDKN1A and ADAM17 expression with a change of left ventricular remodeling echocardiographic parameters in PBMC of patients six months after the first myocardial infarction
SP  - 53
EP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12648
ER  - 

@conference{
author = "Kuveljić, Jovana and Životić, Ivan and Dekleva, Milica and Živković, Maja and Đurić, Tamara",
year = "2023",
abstract = "Introduction: Myocardial infarction (MI) and consequential ischemia with cardiomyocyte loss are followed by left ventricular (LV) remodeling. LV remodeling is crucial process for cardiac function preservation, although when prolonged it can become maladaptive and lead to impaired systolic function and further cardiovascular complications. Echocardiographic parameters are used as a measure of LV structure and function. ADAM17 (a disintegrin and metalloprotease domain) and CDKN1A (cyclindependent kinase inhibitor 1A) have shown regulating role in DNA repair, inflammation, remodeling and fibrosis. The aim of this preliminary study was to investigate the potential effect of CDKN1 and ADAM17 mRNA in post MI heart remodeling. Methods: Sixty four patients with the first MI were prospectively followed-up 6 months after MI. Change (Δ) of echocardiographic parameters within 6 months was calculated as a difference between the value at 6-month follow-up and value at admission. Relative gene expression was detected using the TaqMan® technology. Statistical analyses were done by Statistica 8 software. Results: We have observed correlation between CDKN1A mRNA expression and change of LV enddiastolic diameter (ΔLVEDD, R=0.3, p=0.01) and LV end-systolic diameter (ΔLVESD, R=0.3, p=0.02), but not with LV ejection fraction and stroke volume. ADAM17 expression was not in correlation with analyzed parameters of LV remodeling. However, CDKN1A and ADAM17 mRNA expression in PBMC six months after MI were positively correlated (R=0.6, p<0.001). Conclusion: Preliminary resultssuggest that CDKN1 has a role in post MI LV remodeling, correlating with changes in echocardiographic parameters of LV structure. The validation on a larger sample size is required.",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts",
title = "Correlations of CDKN1A and ADAM17 expression with a change of left ventricular remodeling echocardiographic parameters in PBMC of patients six months after the first myocardial infarction",
pages = "53-53",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12648"
}

Kuveljić, J., Životić, I., Dekleva, M., Živković, M.,& Đurić, T.. (2023). Correlations of CDKN1A and ADAM17 expression with a change of left ventricular remodeling echocardiographic parameters in PBMC of patients six months after the first myocardial infarction. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts, 53-53.
https://hdl.handle.net/21.15107/rcub_vinar_12648

Kuveljić J, Životić I, Dekleva M, Živković M, Đurić T. Correlations of CDKN1A and ADAM17 expression with a change of left ventricular remodeling echocardiographic parameters in PBMC of patients six months after the first myocardial infarction. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts. 2023;:53-53.
https://hdl.handle.net/21.15107/rcub_vinar_12648 .

Kuveljić, Jovana, Životić, Ivan, Dekleva, Milica, Živković, Maja, Đurić, Tamara, "Correlations of CDKN1A and ADAM17 expression with a change of left ventricular remodeling echocardiographic parameters in PBMC of patients six months after the first myocardial infarction" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts (2023):53-53,
https://hdl.handle.net/21.15107/rcub_vinar_12648 .

TY  - CONF
AU  - Dekleva, Milica
AU  - Đurić, Tamara
AU  - Đorđević, Ana
AU  - Soldatović, Ivan A.
AU  - Stanković, Aleksandra
AU  - Stevanović, Angelina
AU  - Živković, Maja
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10710
AB  - Cardiac remodeling after the first myocardial infarction (MI) appears to be more successful in women than in men, but more frequently associated with heart failure (HF) development. Galectin-3 expression is upregulated in remodeling and failing myocardium and circulatory level is activated in hypertrophy, fibrosis and inflammation.This study aimed to investigate the potential role of sex differences in the following: risk factors, structural and functional left ventricle (LV) changes, coronary angiography, expression of Galectin-3 and it's circulating level for HF occurrence during 6 months in patients after first MI.The prospective study included patients with the first MI treated with the primary percutaneous coronary intervention (p PCI) who underwent Doppler echocardiography within 2±1 days of MI and after 6 months. Relative LGALS-3 mRNA expression in peripheral blood were detected by TaqMan® technology. Expression and concentration of Galectin-3 was obtained by ELISA method. Presence of HF was confirmed by clinical examination and Doppler echocardiography. Assessment of p PCI and description of coronary angiography was performed at the patient's admission time.The study included 137 men and 44 women, who were significantly older (57.8 vs. 54.4, p=0.034), with higher LDL cholesterol (3.54±0.93 vs. 4.03±1.27, p=0.027) without differences among angiographic characteristics and medications. In the acute phase of MI, the significantly lower indexed LV volumes were found in women compared to men (EDLVI: 58.3 vs. 49.6, p<0.001, ESLVI: 33.84 vs. 26.83, p<0.001), but the grade of LV remodeling (delta LVDVI, delta LVESVI) during 6 months and changes in LV ejection fraction (deltaLVEF) were similar (p=ns). Incidence of LV hypertrophy and HF development was significantly higher in women 70% vs. 44.6%, p=0.034, 37.5% vs.19.3%, p=0.02). Females have had a higher degree of LV diastolic dysfunction (DD) in the early and late phase after MI (p=0.038, p=0.027). There were significant correlations between grade of DD and level of Gal-3 expression (p=0.001). The relative expression of LGALS-3 mRNA in peripheral blood was higher in females (p=0.007) with upregulation of circulating Gal-3 in females (44.66 vs. 16.30, p<0.001) and in HF patients (31.1 vs. 21.2, p=0.025).Sex specific actions such as LV hypertrophy, diastolic dysfunction, upregulation of Galectin-3 expression and higher circulating level may explain more incidence of HF in female. Difference in model and determinants of HF between men and women can be important for further therapy including Gelectin-3 inhibition.Type of funding sources: None.
C3  - European Heart Journal
T1  - Sex related difference in heart failure development in patients after first myocardial infarction; role of galectine-3
VL  - 43
IS  - Supplement 2
SP  - ehac544.816
DO  - 10.1093/eurheartj/ehac544.816
ER  - 

@conference{
author = "Dekleva, Milica and Đurić, Tamara and Đorđević, Ana and Soldatović, Ivan A. and Stanković, Aleksandra and Stevanović, Angelina and Živković, Maja",
year = "2022",
abstract = "Cardiac remodeling after the first myocardial infarction (MI) appears to be more successful in women than in men, but more frequently associated with heart failure (HF) development. Galectin-3 expression is upregulated in remodeling and failing myocardium and circulatory level is activated in hypertrophy, fibrosis and inflammation.This study aimed to investigate the potential role of sex differences in the following: risk factors, structural and functional left ventricle (LV) changes, coronary angiography, expression of Galectin-3 and it's circulating level for HF occurrence during 6 months in patients after first MI.The prospective study included patients with the first MI treated with the primary percutaneous coronary intervention (p PCI) who underwent Doppler echocardiography within 2±1 days of MI and after 6 months. Relative LGALS-3 mRNA expression in peripheral blood were detected by TaqMan® technology. Expression and concentration of Galectin-3 was obtained by ELISA method. Presence of HF was confirmed by clinical examination and Doppler echocardiography. Assessment of p PCI and description of coronary angiography was performed at the patient's admission time.The study included 137 men and 44 women, who were significantly older (57.8 vs. 54.4, p=0.034), with higher LDL cholesterol (3.54±0.93 vs. 4.03±1.27, p=0.027) without differences among angiographic characteristics and medications. In the acute phase of MI, the significantly lower indexed LV volumes were found in women compared to men (EDLVI: 58.3 vs. 49.6, p<0.001, ESLVI: 33.84 vs. 26.83, p<0.001), but the grade of LV remodeling (delta LVDVI, delta LVESVI) during 6 months and changes in LV ejection fraction (deltaLVEF) were similar (p=ns). Incidence of LV hypertrophy and HF development was significantly higher in women 70% vs. 44.6%, p=0.034, 37.5% vs.19.3%, p=0.02). Females have had a higher degree of LV diastolic dysfunction (DD) in the early and late phase after MI (p=0.038, p=0.027). There were significant correlations between grade of DD and level of Gal-3 expression (p=0.001). The relative expression of LGALS-3 mRNA in peripheral blood was higher in females (p=0.007) with upregulation of circulating Gal-3 in females (44.66 vs. 16.30, p<0.001) and in HF patients (31.1 vs. 21.2, p=0.025).Sex specific actions such as LV hypertrophy, diastolic dysfunction, upregulation of Galectin-3 expression and higher circulating level may explain more incidence of HF in female. Difference in model and determinants of HF between men and women can be important for further therapy including Gelectin-3 inhibition.Type of funding sources: None.",
journal = "European Heart Journal",
title = "Sex related difference in heart failure development in patients after first myocardial infarction; role of galectine-3",
volume = "43",
number = "Supplement 2",
pages = "ehac544.816",
doi = "10.1093/eurheartj/ehac544.816"
}

Dekleva, M., Đurić, T., Đorđević, A., Soldatović, I. A., Stanković, A., Stevanović, A.,& Živković, M.. (2022). Sex related difference in heart failure development in patients after first myocardial infarction; role of galectine-3. in European Heart Journal, 43(Supplement 2), ehac544.816.
https://doi.org/10.1093/eurheartj/ehac544.816

Dekleva M, Đurić T, Đorđević A, Soldatović IA, Stanković A, Stevanović A, Živković M. Sex related difference in heart failure development in patients after first myocardial infarction; role of galectine-3. in European Heart Journal. 2022;43(Supplement 2):ehac544.816.
doi:10.1093/eurheartj/ehac544.816 .

Dekleva, Milica, Đurić, Tamara, Đorđević, Ana, Soldatović, Ivan A., Stanković, Aleksandra, Stevanović, Angelina, Živković, Maja, "Sex related difference in heart failure development in patients after first myocardial infarction; role of galectine-3" in European Heart Journal, 43, no. Supplement 2 (2022):ehac544.816,
https://doi.org/10.1093/eurheartj/ehac544.816 . .

TY  - JOUR
AU  - Đurić, Tamara
AU  - Kuveljić, Jovana
AU  - Đorđević, Ana
AU  - Dekleva, Milica
AU  - Stanković, Goran
AU  - Stanković, Aleksandra
AU  - Živković, Maja
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10382
AB  - Background Myocardial infarction (MI) leads to ischemia and afterward to left ventricular (LV) remodeling. Matrix metalloproteinase−1 (MMP1) and −3 (MMP3) belong to the family of endopeptidases and together they can dissolve most of the components of the extracellular matrix. MMP1 and MMP3 variants have been investigated solely in association with ischemic heart disease and LV dysfunction, but not in haplotype. The aims of this study were to investigate the association of haplotypes inferred from MMP1 rs1799750 (−1607 1G/2G; NC_000011.9:g.102670497del) and MMP3 rs35068180 (−1612 5A/6A; NC_000011.9:g.102715952dup) with MI and their effect on the change in echocardiographic parameters of LV structure and function in patients within 6 months after MI. Methods The study included 325 patients with the first MI and 283 healthy controls. Gene variants were detected by PCR-RFLP method. Parameters of LV structure and function were assessed by conventional 2D echocardiography, 3–5 days and 6 months after the first MI, on a subgroup of 160 patients. Haplotype analysis was performed with Thesias software. Results Haplotypes 2G-5A and 1G-6A were significantly and independently associated with MI compared with the reference haplotype 2G-6A (adjusted, p = 0.009 and p = 0.026, respectively). After Bonferroni correction for multiple testing, MMP1 and MMP3 haplotypes lost their association with the change in LV long diameter and stroke volume within 6 months after MI. Conclusion MMP1 and MMP3 haplotypes are strongly associated with MI. Further studies are needed to validate this result and to examine their association with echocardiographic parameters of LV structure and function after MI.
T2  - Molecular Genetics & Genomic Medicine
T1  - Association of MMP1 and MMP3 haplotypes with myocardial infarction and echocardiographic parameters of the left ventricle
VL  - 10
IS  - 9
SP  - e2022
DO  - 10.1002/mgg3.2022
ER  - 

@article{
author = "Đurić, Tamara and Kuveljić, Jovana and Đorđević, Ana and Dekleva, Milica and Stanković, Goran and Stanković, Aleksandra and Živković, Maja",
year = "2022",
abstract = "Background Myocardial infarction (MI) leads to ischemia and afterward to left ventricular (LV) remodeling. Matrix metalloproteinase−1 (MMP1) and −3 (MMP3) belong to the family of endopeptidases and together they can dissolve most of the components of the extracellular matrix. MMP1 and MMP3 variants have been investigated solely in association with ischemic heart disease and LV dysfunction, but not in haplotype. The aims of this study were to investigate the association of haplotypes inferred from MMP1 rs1799750 (−1607 1G/2G; NC_000011.9:g.102670497del) and MMP3 rs35068180 (−1612 5A/6A; NC_000011.9:g.102715952dup) with MI and their effect on the change in echocardiographic parameters of LV structure and function in patients within 6 months after MI. Methods The study included 325 patients with the first MI and 283 healthy controls. Gene variants were detected by PCR-RFLP method. Parameters of LV structure and function were assessed by conventional 2D echocardiography, 3–5 days and 6 months after the first MI, on a subgroup of 160 patients. Haplotype analysis was performed with Thesias software. Results Haplotypes 2G-5A and 1G-6A were significantly and independently associated with MI compared with the reference haplotype 2G-6A (adjusted, p = 0.009 and p = 0.026, respectively). After Bonferroni correction for multiple testing, MMP1 and MMP3 haplotypes lost their association with the change in LV long diameter and stroke volume within 6 months after MI. Conclusion MMP1 and MMP3 haplotypes are strongly associated with MI. Further studies are needed to validate this result and to examine their association with echocardiographic parameters of LV structure and function after MI.",
journal = "Molecular Genetics & Genomic Medicine",
title = "Association of MMP1 and MMP3 haplotypes with myocardial infarction and echocardiographic parameters of the left ventricle",
volume = "10",
number = "9",
pages = "e2022",
doi = "10.1002/mgg3.2022"
}

Đurić, T., Kuveljić, J., Đorđević, A., Dekleva, M., Stanković, G., Stanković, A.,& Živković, M.. (2022). Association of MMP1 and MMP3 haplotypes with myocardial infarction and echocardiographic parameters of the left ventricle. in Molecular Genetics & Genomic Medicine, 10(9), e2022.
https://doi.org/10.1002/mgg3.2022

Đurić T, Kuveljić J, Đorđević A, Dekleva M, Stanković G, Stanković A, Živković M. Association of MMP1 and MMP3 haplotypes with myocardial infarction and echocardiographic parameters of the left ventricle. in Molecular Genetics & Genomic Medicine. 2022;10(9):e2022.
doi:10.1002/mgg3.2022 .

Đurić, Tamara, Kuveljić, Jovana, Đorđević, Ana, Dekleva, Milica, Stanković, Goran, Stanković, Aleksandra, Živković, Maja, "Association of MMP1 and MMP3 haplotypes with myocardial infarction and echocardiographic parameters of the left ventricle" in Molecular Genetics & Genomic Medicine, 10, no. 9 (2022):e2022,
https://doi.org/10.1002/mgg3.2022 . .

TY  - JOUR
AU  - Živković, Maja
AU  - Bubić, Maja
AU  - Kolaković, Ana
AU  - Dekleva, Milica
AU  - Stanković, Goran
AU  - Stanković, Aleksandra
AU  - Đurić, Tamara
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9842
AB  - Glutathione S-transferases (GSTs) are the family of enzymes involved in the second line of defense against oxidative stress (OS). The lack of GSTT1/GSTM1 enzyme quantity or activity, due to the presence of homozygous deletion compromises antioxidative defense resulting in OS. OS is the critical mechanism in the pathophysiology of atherosclerosis, coronary artery disease, and myocardial infarction (MI). The increase in reactive oxygen species together with the process of apoptosis plays a role in left ventricular remodeling (LVR) after MI. The associations of GSTT1 and GSTM1 gene polymorphisms with the risk of MI are inconsistent. The aim was to analyze the association of GSTT1/GSTM1 null genotypes with first MI and LVR 8 months after the MI. The study involved 330 controls and 438 consecutive patients with symptoms and signs of first MI. The subgroup of 150 MI patients was prospectively followed up for 6 months. Evidence of maladaptive LVR was obtained by 2D Doppler echocardiography 3-5 days and 6 months after the MI. A multiplex polymerase chain reaction was used to detect the deletion in GSTT1 and GSTM1 genes. GSTM1 null genotype was significantly and independently associated with first MI (adjusted OR = 1.45 95% CI 1.03-2.03, p = 0.03). Association of double null genotypes with maladaptive LVR in patients 6 months after the first MI was no longer significant after adjustment for factors that differed significantly between patients with and without maladaptive LVR. This study demonstrated the association of GSTM1 null genotypes with the risk of MI in the Serbian population.
T2  - Free Radical Research
T1  - The association of glutathione S-transferase T1 and M1 deletions with myocardial infarction
VL  - 55
IS  - 3
SP  - 267
EP  - 274
DO  - 10.1080/10715762.2021.1931166
ER  - 

@article{
author = "Živković, Maja and Bubić, Maja and Kolaković, Ana and Dekleva, Milica and Stanković, Goran and Stanković, Aleksandra and Đurić, Tamara",
year = "2021",
abstract = "Glutathione S-transferases (GSTs) are the family of enzymes involved in the second line of defense against oxidative stress (OS). The lack of GSTT1/GSTM1 enzyme quantity or activity, due to the presence of homozygous deletion compromises antioxidative defense resulting in OS. OS is the critical mechanism in the pathophysiology of atherosclerosis, coronary artery disease, and myocardial infarction (MI). The increase in reactive oxygen species together with the process of apoptosis plays a role in left ventricular remodeling (LVR) after MI. The associations of GSTT1 and GSTM1 gene polymorphisms with the risk of MI are inconsistent. The aim was to analyze the association of GSTT1/GSTM1 null genotypes with first MI and LVR 8 months after the MI. The study involved 330 controls and 438 consecutive patients with symptoms and signs of first MI. The subgroup of 150 MI patients was prospectively followed up for 6 months. Evidence of maladaptive LVR was obtained by 2D Doppler echocardiography 3-5 days and 6 months after the MI. A multiplex polymerase chain reaction was used to detect the deletion in GSTT1 and GSTM1 genes. GSTM1 null genotype was significantly and independently associated with first MI (adjusted OR = 1.45 95% CI 1.03-2.03, p = 0.03). Association of double null genotypes with maladaptive LVR in patients 6 months after the first MI was no longer significant after adjustment for factors that differed significantly between patients with and without maladaptive LVR. This study demonstrated the association of GSTM1 null genotypes with the risk of MI in the Serbian population.",
journal = "Free Radical Research",
title = "The association of glutathione S-transferase T1 and M1 deletions with myocardial infarction",
volume = "55",
number = "3",
pages = "267-274",
doi = "10.1080/10715762.2021.1931166"
}

Živković, M., Bubić, M., Kolaković, A., Dekleva, M., Stanković, G., Stanković, A.,& Đurić, T.. (2021). The association of glutathione S-transferase T1 and M1 deletions with myocardial infarction. in Free Radical Research, 55(3), 267-274.
https://doi.org/10.1080/10715762.2021.1931166

Živković M, Bubić M, Kolaković A, Dekleva M, Stanković G, Stanković A, Đurić T. The association of glutathione S-transferase T1 and M1 deletions with myocardial infarction. in Free Radical Research. 2021;55(3):267-274.
doi:10.1080/10715762.2021.1931166 .

Živković, Maja, Bubić, Maja, Kolaković, Ana, Dekleva, Milica, Stanković, Goran, Stanković, Aleksandra, Đurić, Tamara, "The association of glutathione S-transferase T1 and M1 deletions with myocardial infarction" in Free Radical Research, 55, no. 3 (2021):267-274,
https://doi.org/10.1080/10715762.2021.1931166 . .

TY  - CONF
AU  - Đorđević, Ana M.
AU  - Dekleva, Milica
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Kuveljić, Jovana
AU  - Đurić, Tamara
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12729
AB  - After myocardial infarction (MI) and consequential ischemia, the heart undergoes a set of geometric and functional changes. In the early days after injury, these changes, defined as cardiac remodeling, are the powerful factor that preserves cardiac function and promotes survival. However, it may continue for months after MI and eventually lead to adverse remodeling with impaired systolic function and reduced myocardial contractility and further cardiovascular complications, such as heart failure (HF). Left ventricular (LV) ejection fraction (EF) is widely used as an index of systolic function in cardiac patients. However, global myocardial strain has been found to be superior to the conventional parameters, such as LVEF, in terms of assessment of cardiac performance after MI. Galectin-3 (gal-3) is a multifunctional protein involved in a variety of physiological and pathological processes, affecting the entire cardiovascular continuum of MI. Gal-3 is encoded by a LGALS-3 gene, located in a unique, 300 kb long haplotype block in Caucasians. Gal-3 serum level has been approved as a diagnostic marker for risk stratification and prognosis evaluation of HF patients according to the ACC/AHA/HFSA Guideline for the management of HF. The purpose of the present prospective study was to analyze the possible association of tag genetic variants of the haplotype block containing LGALS-3 with changes in cardiac parameters, LVEF and global radial strain (GRS), within 6 months post-MI. The study enrolled 120 patients with first acute MI that were prospectively followed-up 6 months after MI. According to Tagger server, rs4040064 G/T, rs11628437 G/A and rs7159490 C/T variants cover 82% (r2>0.8) of phenotypic variance of the aforementioned haplotype block. Tag variants were detected and genotyped by commercially available assays for allelic discrimination. Echocardiography examinations were performed at admission and 6 months post-MI. Change (Δ) of cardiac parameters was calculated as a difference between the value at 6-month follow-up and baseline value (at admission). The referent haplotype is set by the software for carrying haplotype association analysis and represents the most frequent haplotype in the studied groups. Bonferroni correction for multiple testing was performed and p values <0.025 were considered as statistically significant.We found that, compared to the reference GGC haplotype, GAT haplotype had significantly higher expected phenotypic mean [95% CI] of ΔGRS (3.77 [1.28 - 6.25] vs. −5.34 [−12.69 - 2.01], respectively, p=0.025) and ΔLVEF (0.84 [−1.88 - 3.56] vs. −12.91 [−17.30 - −8.53], respectively, p=0.00001), in the direction of decrease of GRS and LVEF 6 months after MI in patients bearing GAT haplotype. Our findings suggest that GAT haplotype bears the risk for diminished LV transmural contractility and radial systolic function: In order to reach a definitive conclusion, our exploratory results should be further validated on a larger sample
C3  - European Journal of Hear Failure
T1  - LGALS-3 containing haplotype block tag variants in association with cardiac parameters changes within six months after the first acute myocardial infarction
VL  - 23
IS  - Suppl. S2
SP  - 311
EP  - 311
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12729
ER  - 

@conference{
author = "Đorđević, Ana M. and Dekleva, Milica and Živković, Maja and Stanković, Aleksandra and Kuveljić, Jovana and Đurić, Tamara",
year = "2021",
abstract = "After myocardial infarction (MI) and consequential ischemia, the heart undergoes a set of geometric and functional changes. In the early days after injury, these changes, defined as cardiac remodeling, are the powerful factor that preserves cardiac function and promotes survival. However, it may continue for months after MI and eventually lead to adverse remodeling with impaired systolic function and reduced myocardial contractility and further cardiovascular complications, such as heart failure (HF). Left ventricular (LV) ejection fraction (EF) is widely used as an index of systolic function in cardiac patients. However, global myocardial strain has been found to be superior to the conventional parameters, such as LVEF, in terms of assessment of cardiac performance after MI. Galectin-3 (gal-3) is a multifunctional protein involved in a variety of physiological and pathological processes, affecting the entire cardiovascular continuum of MI. Gal-3 is encoded by a LGALS-3 gene, located in a unique, 300 kb long haplotype block in Caucasians. Gal-3 serum level has been approved as a diagnostic marker for risk stratification and prognosis evaluation of HF patients according to the ACC/AHA/HFSA Guideline for the management of HF. The purpose of the present prospective study was to analyze the possible association of tag genetic variants of the haplotype block containing LGALS-3 with changes in cardiac parameters, LVEF and global radial strain (GRS), within 6 months post-MI. The study enrolled 120 patients with first acute MI that were prospectively followed-up 6 months after MI. According to Tagger server, rs4040064 G/T, rs11628437 G/A and rs7159490 C/T variants cover 82% (r2>0.8) of phenotypic variance of the aforementioned haplotype block. Tag variants were detected and genotyped by commercially available assays for allelic discrimination. Echocardiography examinations were performed at admission and 6 months post-MI. Change (Δ) of cardiac parameters was calculated as a difference between the value at 6-month follow-up and baseline value (at admission). The referent haplotype is set by the software for carrying haplotype association analysis and represents the most frequent haplotype in the studied groups. Bonferroni correction for multiple testing was performed and p values <0.025 were considered as statistically significant.We found that, compared to the reference GGC haplotype, GAT haplotype had significantly higher expected phenotypic mean [95% CI] of ΔGRS (3.77 [1.28 - 6.25] vs. −5.34 [−12.69 - 2.01], respectively, p=0.025) and ΔLVEF (0.84 [−1.88 - 3.56] vs. −12.91 [−17.30 - −8.53], respectively, p=0.00001), in the direction of decrease of GRS and LVEF 6 months after MI in patients bearing GAT haplotype. Our findings suggest that GAT haplotype bears the risk for diminished LV transmural contractility and radial systolic function: In order to reach a definitive conclusion, our exploratory results should be further validated on a larger sample",
journal = "European Journal of Hear Failure",
title = "LGALS-3 containing haplotype block tag variants in association with cardiac parameters changes within six months after the first acute myocardial infarction",
volume = "23",
number = "Suppl. S2",
pages = "311-311",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12729"
}

Đorđević, A. M., Dekleva, M., Živković, M., Stanković, A., Kuveljić, J.,& Đurić, T.. (2021). LGALS-3 containing haplotype block tag variants in association with cardiac parameters changes within six months after the first acute myocardial infarction. in European Journal of Hear Failure, 23(Suppl. S2), 311-311.
https://hdl.handle.net/21.15107/rcub_vinar_12729

Đorđević AM, Dekleva M, Živković M, Stanković A, Kuveljić J, Đurić T. LGALS-3 containing haplotype block tag variants in association with cardiac parameters changes within six months after the first acute myocardial infarction. in European Journal of Hear Failure. 2021;23(Suppl. S2):311-311.
https://hdl.handle.net/21.15107/rcub_vinar_12729 .

Đorđević, Ana M., Dekleva, Milica, Živković, Maja, Stanković, Aleksandra, Kuveljić, Jovana, Đurić, Tamara, "LGALS-3 containing haplotype block tag variants in association with cardiac parameters changes within six months after the first acute myocardial infarction" in European Journal of Hear Failure, 23, no. Suppl. S2 (2021):311-311,
https://hdl.handle.net/21.15107/rcub_vinar_12729 .

TY  - JOUR
AU  - Kuveljić, Jovana
AU  - Đurić, Tamara
AU  - Stanković, Goran
AU  - Dekleva, Milica
AU  - Stanković, Aleksandra
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9541
AB  - Background: Myocardial infarction (MI) and underlining atherosclerosis are the main causes of death worldwide. Phosphatase and actin regulator 1 (PHACTR1) variants have been associated with early onset MI, coronary artery disease and carotid dissection. PHACTR1 mRNA expression has been detected in tissues and cells related to atherosclerosis. Nonetheless, the true effect of PHACTR1 on vascular diseases is still unknown. Our aim was to examine the association of PHACTR1 intronic variants, rs9349379, rs2026458 and rs2876300, with MI and multi-vessel disease (MVD) and to assess their effect on PHACTR1 and EDN1 mRNA expression in PBMCs of patients six months after MI. Methods: The study enrolled 537 patients with the first MI and 310 controls. Gene expression was assessed in 74 patients six months after MI and 37 healthy controls. Rs9349379, rs2026458, rs2876300 and relative mRNA expressions were detected by TaqMan® technology. Results: The significant association between PHACTR1 variants and MI was not found, either individually or in haplotype. A higher frequency of rs2876300G-allele in MVD was rendered not significant after Bonferroni correction. PHACTR1 mRNA was significantly increased in PBMCs of patients six months after MI compared to controls (p = 0.02). Patients that carry ACG haplotype have increased PHACTR1 mRNA expression in PBMCs (p = 0.04). There was no effect of PHACTR1 variants on EDN1 mRNA expression. Conclusion: Our findings suggest that PHACTR1 intronic variants may have a role in severity and progression of coronary atherosclerosis. Future research is needed to clarify the mechanism underlying the role of PHACTR1 in coronary atherosclerosis and MI. © 2021 Elsevier B.V.
T2  - Gene
T1  - Association of PHACTR1 intronic variants with the first myocardial infarction and their effect on PHACTR1 mRNA expression in PBMCs
VL  - 775
SP  - 145428
DO  - 10.1016/j.gene.2021.145428
ER  - 

@article{
author = "Kuveljić, Jovana and Đurić, Tamara and Stanković, Goran and Dekleva, Milica and Stanković, Aleksandra and Alavantić, Dragan and Živković, Maja",
year = "2021",
abstract = "Background: Myocardial infarction (MI) and underlining atherosclerosis are the main causes of death worldwide. Phosphatase and actin regulator 1 (PHACTR1) variants have been associated with early onset MI, coronary artery disease and carotid dissection. PHACTR1 mRNA expression has been detected in tissues and cells related to atherosclerosis. Nonetheless, the true effect of PHACTR1 on vascular diseases is still unknown. Our aim was to examine the association of PHACTR1 intronic variants, rs9349379, rs2026458 and rs2876300, with MI and multi-vessel disease (MVD) and to assess their effect on PHACTR1 and EDN1 mRNA expression in PBMCs of patients six months after MI. Methods: The study enrolled 537 patients with the first MI and 310 controls. Gene expression was assessed in 74 patients six months after MI and 37 healthy controls. Rs9349379, rs2026458, rs2876300 and relative mRNA expressions were detected by TaqMan® technology. Results: The significant association between PHACTR1 variants and MI was not found, either individually or in haplotype. A higher frequency of rs2876300G-allele in MVD was rendered not significant after Bonferroni correction. PHACTR1 mRNA was significantly increased in PBMCs of patients six months after MI compared to controls (p = 0.02). Patients that carry ACG haplotype have increased PHACTR1 mRNA expression in PBMCs (p = 0.04). There was no effect of PHACTR1 variants on EDN1 mRNA expression. Conclusion: Our findings suggest that PHACTR1 intronic variants may have a role in severity and progression of coronary atherosclerosis. Future research is needed to clarify the mechanism underlying the role of PHACTR1 in coronary atherosclerosis and MI. © 2021 Elsevier B.V.",
journal = "Gene",
title = "Association of PHACTR1 intronic variants with the first myocardial infarction and their effect on PHACTR1 mRNA expression in PBMCs",
volume = "775",
pages = "145428",
doi = "10.1016/j.gene.2021.145428"
}

Kuveljić, J., Đurić, T., Stanković, G., Dekleva, M., Stanković, A., Alavantić, D.,& Živković, M.. (2021). Association of PHACTR1 intronic variants with the first myocardial infarction and their effect on PHACTR1 mRNA expression in PBMCs. in Gene, 775, 145428.
https://doi.org/10.1016/j.gene.2021.145428

Kuveljić J, Đurić T, Stanković G, Dekleva M, Stanković A, Alavantić D, Živković M. Association of PHACTR1 intronic variants with the first myocardial infarction and their effect on PHACTR1 mRNA expression in PBMCs. in Gene. 2021;775:145428.
doi:10.1016/j.gene.2021.145428 .

Kuveljić, Jovana, Đurić, Tamara, Stanković, Goran, Dekleva, Milica, Stanković, Aleksandra, Alavantić, Dragan, Živković, Maja, "Association of PHACTR1 intronic variants with the first myocardial infarction and their effect on PHACTR1 mRNA expression in PBMCs" in Gene, 775 (2021):145428,
https://doi.org/10.1016/j.gene.2021.145428 . .

TY  - JOUR
AU  - Životić, Ivan
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Milašinović, Dejan
AU  - Stanković, Goran
AU  - Dekleva, Milica
AU  - Marković-Nikolić, Nataša
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8432
AB  - Background: Chromosomal region 9p21.3 is most robustly associated with coronary artery disease (CAD) in western European populations. However, heterogeneity in CAD phenotypes leads to uncertainty whether 9p21.3 is associated with stable and/or acute clinical presentations of CAD. 9p21.3 is rich in regulatory elements, but the underlying mechanisms of its actions in CAD remain unclear. We investigate the association of 9p21.3 two haplotype blocks lead variants (rs10757278 and rs518394) with first-ever non-fatal myocardial infarction (MI) in CAD patients and their association with CDKN2B mRNA expression in peripheral blood mononuclear cells 6 months after the event. Methods: We included CAD patients with sustained first MI (n = 523) and controls (n = 583). Gene expression was assessed in 72 patients 6 months after MI and 43 healthy controls. TaqMan® technology was used for the gene expression and genotyping analysis. Results: CDKN2B mRNA was significantly lower in MI patients compared with the controls (p = 0.002) and in patients carrying the rs10757278 G risk allele versus AA homozygotes (p = 0.012) 6 months after the event. While we confirmed the association of rs10757278 with CDKN2B expression in MI patients, we failed to find an association between the investigated variants and MI or disease burden. Conclusions: We suggest a dysregulation of gene expression in the 9p21.3 region six months after acute MI, which is affected by a genetic variant in patients. The rs10757278 rare allele is one factor that might lead to prolonged risk for proatherogenic complications. © 2019 The Canadian Society of Clinical Chemists
T2  - Clinical Biochemistry
T1  - CDKN2B gene expression is affected by 9p21.3 rs10757278 in CAD patients, six months after the MI
VL  - 73
SP  - 70
EP  - 76
DO  - 10.1016/j.clinbiochem.2019.08.003
ER  - 

@article{
author = "Životić, Ivan and Đurić, Tamara and Stanković, Aleksandra and Milašinović, Dejan and Stanković, Goran and Dekleva, Milica and Marković-Nikolić, Nataša and Alavantić, Dragan and Živković, Maja",
year = "2019",
abstract = "Background: Chromosomal region 9p21.3 is most robustly associated with coronary artery disease (CAD) in western European populations. However, heterogeneity in CAD phenotypes leads to uncertainty whether 9p21.3 is associated with stable and/or acute clinical presentations of CAD. 9p21.3 is rich in regulatory elements, but the underlying mechanisms of its actions in CAD remain unclear. We investigate the association of 9p21.3 two haplotype blocks lead variants (rs10757278 and rs518394) with first-ever non-fatal myocardial infarction (MI) in CAD patients and their association with CDKN2B mRNA expression in peripheral blood mononuclear cells 6 months after the event. Methods: We included CAD patients with sustained first MI (n = 523) and controls (n = 583). Gene expression was assessed in 72 patients 6 months after MI and 43 healthy controls. TaqMan® technology was used for the gene expression and genotyping analysis. Results: CDKN2B mRNA was significantly lower in MI patients compared with the controls (p = 0.002) and in patients carrying the rs10757278 G risk allele versus AA homozygotes (p = 0.012) 6 months after the event. While we confirmed the association of rs10757278 with CDKN2B expression in MI patients, we failed to find an association between the investigated variants and MI or disease burden. Conclusions: We suggest a dysregulation of gene expression in the 9p21.3 region six months after acute MI, which is affected by a genetic variant in patients. The rs10757278 rare allele is one factor that might lead to prolonged risk for proatherogenic complications. © 2019 The Canadian Society of Clinical Chemists",
journal = "Clinical Biochemistry",
title = "CDKN2B gene expression is affected by 9p21.3 rs10757278 in CAD patients, six months after the MI",
volume = "73",
pages = "70-76",
doi = "10.1016/j.clinbiochem.2019.08.003"
}

Životić, I., Đurić, T., Stanković, A., Milašinović, D., Stanković, G., Dekleva, M., Marković-Nikolić, N., Alavantić, D.,& Živković, M.. (2019). CDKN2B gene expression is affected by 9p21.3 rs10757278 in CAD patients, six months after the MI. in Clinical Biochemistry, 73, 70-76.
https://doi.org/10.1016/j.clinbiochem.2019.08.003

Životić I, Đurić T, Stanković A, Milašinović D, Stanković G, Dekleva M, Marković-Nikolić N, Alavantić D, Živković M. CDKN2B gene expression is affected by 9p21.3 rs10757278 in CAD patients, six months after the MI. in Clinical Biochemistry. 2019;73:70-76.
doi:10.1016/j.clinbiochem.2019.08.003 .

Životić, Ivan, Đurić, Tamara, Stanković, Aleksandra, Milašinović, Dejan, Stanković, Goran, Dekleva, Milica, Marković-Nikolić, Nataša, Alavantić, Dragan, Živković, Maja, "CDKN2B gene expression is affected by 9p21.3 rs10757278 in CAD patients, six months after the MI" in Clinical Biochemistry, 73 (2019):70-76,
https://doi.org/10.1016/j.clinbiochem.2019.08.003 . .

TY  - CONF
AU  - Životić, Ivan
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Stanković, Goran
AU  - Milašinović, Dejan
AU  - Dekleva, Milica
AU  - Marković-Nikolić, Nataša
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0021915018309699
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7953
AB  - Aim: Myocardial infarction (MI) incidence is still a major burden in the
terms of premature death and disability world-wide. Genetic studies up to
date explained a limited portion of disease inheritance. Recently, the DT
haplotype of variants rs36212560 I/D (insertion/deletion) and 2275888 T/C
in HACD gene (9p21.3) have been significantly associated with the risk of
carotid plaque occurrence among males.
Methods: We aimed to validate these results by investigating 518 MI patients (366 males) and 303 (167 males) healthy controls from Serbia. Also, he HACD4 gene expression analysis has been conducted in the peripheral
blood mononuclear cells of 72 MI patients, 6 months after the MI. Results
were obtained using ABI-Prism 7500 RT-PCR for rs2275888 allelic
discrimination and HACD4 mRNA relative quantitation. PCR and acrylamide gel electrophoresis were used to distinguish 5 base pairs
rs36212560 insertion deletion polymorphisms.
Results: Haplotype analysis (using Thesias software) showed that DT
haplotype carriers had significantly higher risk for MI (OR ¼ 1.42, CI 1.08-
1.85, p¼0.01) compared with most frequent IT haplotype. In gender
separated groups association remained significant only among males (OR
¼ 1.76, 1.266-2.46, p¼0.0008). Results were adjusted for MI traditional risk
factors (Age, BMI, HDLC, LDLC, Tg, hypertension and smoking). Investigated
genetic variants were not associated with HACD4 expression. Significant
correlation was found between HACD4 mRNA level and age (r¼0.36,
p¼0.001).
Conclusions: We have shown that rs36212560 and rs2275888 DT haplotype from HACD4 gene is significantly and independently associated with
the MI occurrence in males. Additional studies are needed to confirm these
results.
C3  - Atherosclerosis
T1  - HACD4 haplotype confers risk of myocardial infarction among males in the population of Serbia
VL  - 275
SP  - e210
EP  - e211
DO  - 10.1016/j.atherosclerosis.2018.06.657
ER  - 

@conference{
author = "Životić, Ivan and Đurić, Tamara and Stanković, Aleksandra and Stanković, Goran and Milašinović, Dejan and Dekleva, Milica and Marković-Nikolić, Nataša and Alavantić, Dragan and Živković, Maja",
year = "2018",
abstract = "Aim: Myocardial infarction (MI) incidence is still a major burden in the
terms of premature death and disability world-wide. Genetic studies up to
date explained a limited portion of disease inheritance. Recently, the DT
haplotype of variants rs36212560 I/D (insertion/deletion) and 2275888 T/C
in HACD gene (9p21.3) have been significantly associated with the risk of
carotid plaque occurrence among males.
Methods: We aimed to validate these results by investigating 518 MI patients (366 males) and 303 (167 males) healthy controls from Serbia. Also, he HACD4 gene expression analysis has been conducted in the peripheral
blood mononuclear cells of 72 MI patients, 6 months after the MI. Results
were obtained using ABI-Prism 7500 RT-PCR for rs2275888 allelic
discrimination and HACD4 mRNA relative quantitation. PCR and acrylamide gel electrophoresis were used to distinguish 5 base pairs
rs36212560 insertion deletion polymorphisms.
Results: Haplotype analysis (using Thesias software) showed that DT
haplotype carriers had significantly higher risk for MI (OR ¼ 1.42, CI 1.08-
1.85, p¼0.01) compared with most frequent IT haplotype. In gender
separated groups association remained significant only among males (OR
¼ 1.76, 1.266-2.46, p¼0.0008). Results were adjusted for MI traditional risk
factors (Age, BMI, HDLC, LDLC, Tg, hypertension and smoking). Investigated
genetic variants were not associated with HACD4 expression. Significant
correlation was found between HACD4 mRNA level and age (r¼0.36,
p¼0.001).
Conclusions: We have shown that rs36212560 and rs2275888 DT haplotype from HACD4 gene is significantly and independently associated with
the MI occurrence in males. Additional studies are needed to confirm these
results.",
journal = "Atherosclerosis",
title = "HACD4 haplotype confers risk of myocardial infarction among males in the population of Serbia",
volume = "275",
pages = "e210-e211",
doi = "10.1016/j.atherosclerosis.2018.06.657"
}

Životić, I., Đurić, T., Stanković, A., Stanković, G., Milašinović, D., Dekleva, M., Marković-Nikolić, N., Alavantić, D.,& Živković, M.. (2018). HACD4 haplotype confers risk of myocardial infarction among males in the population of Serbia. in Atherosclerosis, 275, e210-e211.
https://doi.org/10.1016/j.atherosclerosis.2018.06.657

Životić I, Đurić T, Stanković A, Stanković G, Milašinović D, Dekleva M, Marković-Nikolić N, Alavantić D, Živković M. HACD4 haplotype confers risk of myocardial infarction among males in the population of Serbia. in Atherosclerosis. 2018;275:e210-e211.
doi:10.1016/j.atherosclerosis.2018.06.657 .

Životić, Ivan, Đurić, Tamara, Stanković, Aleksandra, Stanković, Goran, Milašinović, Dejan, Dekleva, Milica, Marković-Nikolić, Nataša, Alavantić, Dragan, Živković, Maja, "HACD4 haplotype confers risk of myocardial infarction among males in the population of Serbia" in Atherosclerosis, 275 (2018):e210-e211,
https://doi.org/10.1016/j.atherosclerosis.2018.06.657 . .

TY  - JOUR
AU  - Đorđević, Ana D.
AU  - Dekleva, Milica
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Marković-Nikolić, Nataša
AU  - Alavantić, Dragan
AU  - Đurić, Tamara
PY  - 2018
UR  - http://link.springer.com/10.1007/s11033-018-4384-4
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7958
AB  - Post-infarct left ventricular remodeling (LVR) process increases the risk of heart failure (HF). Circulating galectin-3 has been associated with fibrosis, inflammation and cardiac dysfunction during the remodeling process after myocardial infarction (MI). The aims of this prospective case study were to investigate the association of potentially functional variants in the vicinity of LGALS-3 locus, rs2274273 and rs17128183 with maladaptive LVR and whether these variants could affect LGALS-3 mRNA expression in peripheral blood mononuclear cells of patients 6 months after the first MI. This study encompassed 167 patients with acute MI that were followed up for 6 months. Evidence of LVR was obtained by repeated 2D Doppler echocardiography. Rs2274273, rs17128183 and LGALS-3 mRNA expression were detected by TaqMan® technology. Rs2274273 and rs17128183 rare allele bearing genotypes, according to the dominant model (CT+TT vs. CC and AG+GG vs. AA, respectively), were significantly and independently associated with maladaptive LVR (adjusted OR = 3.02, P = 0.016; adjusted OR = 3.14, P = 0.019, respectively) and higher LGALS-3 mRNA expression (fold induction 1.203, P = 0.03 and 1.214, P = 0.03, respectively). Our exploratory results suggest that rs2274273 and rs17128183 variants affect LGALS-3 mRNA and bear the risk for maladaptive LVR post-MI remodeling. Further replication and validation in a larger group of patients is inevitable. © 2018, Springer Nature B.V.
T2  - Molecular Biology Reports
T1  - Left ventricular remodeling after the first myocardial infarction in association with LGALS-3 neighbouring variants rs2274273 and rs17128183 and its relative mRNA expression: a prospective study
VL  - 45
IS  - 6
SP  - 2227
EP  - 2236
DO  - 10.1007/s11033-018-4384-4
ER  - 

@article{
author = "Đorđević, Ana D. and Dekleva, Milica and Živković, Maja and Stanković, Aleksandra and Marković-Nikolić, Nataša and Alavantić, Dragan and Đurić, Tamara",
year = "2018",
abstract = "Post-infarct left ventricular remodeling (LVR) process increases the risk of heart failure (HF). Circulating galectin-3 has been associated with fibrosis, inflammation and cardiac dysfunction during the remodeling process after myocardial infarction (MI). The aims of this prospective case study were to investigate the association of potentially functional variants in the vicinity of LGALS-3 locus, rs2274273 and rs17128183 with maladaptive LVR and whether these variants could affect LGALS-3 mRNA expression in peripheral blood mononuclear cells of patients 6 months after the first MI. This study encompassed 167 patients with acute MI that were followed up for 6 months. Evidence of LVR was obtained by repeated 2D Doppler echocardiography. Rs2274273, rs17128183 and LGALS-3 mRNA expression were detected by TaqMan® technology. Rs2274273 and rs17128183 rare allele bearing genotypes, according to the dominant model (CT+TT vs. CC and AG+GG vs. AA, respectively), were significantly and independently associated with maladaptive LVR (adjusted OR = 3.02, P = 0.016; adjusted OR = 3.14, P = 0.019, respectively) and higher LGALS-3 mRNA expression (fold induction 1.203, P = 0.03 and 1.214, P = 0.03, respectively). Our exploratory results suggest that rs2274273 and rs17128183 variants affect LGALS-3 mRNA and bear the risk for maladaptive LVR post-MI remodeling. Further replication and validation in a larger group of patients is inevitable. © 2018, Springer Nature B.V.",
journal = "Molecular Biology Reports",
title = "Left ventricular remodeling after the first myocardial infarction in association with LGALS-3 neighbouring variants rs2274273 and rs17128183 and its relative mRNA expression: a prospective study",
volume = "45",
number = "6",
pages = "2227-2236",
doi = "10.1007/s11033-018-4384-4"
}

Đorđević, A. D., Dekleva, M., Živković, M., Stanković, A., Marković-Nikolić, N., Alavantić, D.,& Đurić, T.. (2018). Left ventricular remodeling after the first myocardial infarction in association with LGALS-3 neighbouring variants rs2274273 and rs17128183 and its relative mRNA expression: a prospective study. in Molecular Biology Reports, 45(6), 2227-2236.
https://doi.org/10.1007/s11033-018-4384-4

Đorđević AD, Dekleva M, Živković M, Stanković A, Marković-Nikolić N, Alavantić D, Đurić T. Left ventricular remodeling after the first myocardial infarction in association with LGALS-3 neighbouring variants rs2274273 and rs17128183 and its relative mRNA expression: a prospective study. in Molecular Biology Reports. 2018;45(6):2227-2236.
doi:10.1007/s11033-018-4384-4 .

Đorđević, Ana D., Dekleva, Milica, Živković, Maja, Stanković, Aleksandra, Marković-Nikolić, Nataša, Alavantić, Dragan, Đurić, Tamara, "Left ventricular remodeling after the first myocardial infarction in association with LGALS-3 neighbouring variants rs2274273 and rs17128183 and its relative mRNA expression: a prospective study" in Molecular Biology Reports, 45, no. 6 (2018):2227-2236,
https://doi.org/10.1007/s11033-018-4384-4 . .

TY  - CONF
AU  - Životić, Ivan
AU  - Jovanović, Ivan
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Dekleva, Milica
AU  - Marković Nikolić, Nevena
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12816
AB  - Introduction: Recently, rs2275888 eQTL in PTPLAD2 gene has been associated with expression of several loci, during inflammatory stimulation in monocytes. Myocardial infarction (MI) triggers an intense inflammatory response that is essential for cardiac repair. We aimed to perform data scouting in appropriate rs2275888 genotype model to identify differentially expressed genes (DEGs), their biological meaning, and key miRs potentially associated with rs2275888 eQTL in peripheral blood mononuclear leucocytes (PBML) of MI patients 6 months after first MI. Methods: Transcriptome data was obtained from PBMLs of 21 patients, who suffered ischemic MI, by employing Illumina iScan microarray technology. Genotyping for rs2275888 was conducted with real-time PCR, using TaqMan® assay. Preprocessing and identification of DEGs was done using limma package of R/Bioconductor software. The online tool DAVID v6.8 was employed for functional enrichment analysis. Most important miRs were selected using NetworkAnalyst web tool, based on the degree centrality value. Results: Transcriptome analysis in recessive model TT+TC (n=19) vs. CC (n=2) identified 68 DEGs. Top significant biological processes involving DEGs cover vascular physiology, cell growth and signaling. Pathway analysis associated DEGs with adherens junction, Rap1 and Ras signaling. Network analysis identified hsa-miR335-5p, -26b-5p, -93-5p, -16-5p, -124-3p, -20b-5p, -17-5p and -218-5p as miRs with top centrality degree in our DEGs list. Conclusion: Seven of eight detected miRs have already been described in MI pathology or suggested as potential biomarkers for MI. Our result suggest the importance of integration of eQTLs, biological processes and pathway analysis coupled with miR activity for further research in MI pathology.
PB  - Belgrade : University of Belgrade, Faculty of Biology
C3  - CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts
T1  - Microarray transcriptome profiling in myocardial infarction regarding PTPLAD2 rs2275888 eQTL: a data scouting approach
SP  - 176
EP  - 176
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12816
ER  - 

@conference{
author = "Životić, Ivan and Jovanović, Ivan and Đurić, Tamara and Stanković, Aleksandra and Dekleva, Milica and Marković Nikolić, Nevena and Alavantić, Dragan and Živković, Maja",
year = "2017",
abstract = "Introduction: Recently, rs2275888 eQTL in PTPLAD2 gene has been associated with expression of several loci, during inflammatory stimulation in monocytes. Myocardial infarction (MI) triggers an intense inflammatory response that is essential for cardiac repair. We aimed to perform data scouting in appropriate rs2275888 genotype model to identify differentially expressed genes (DEGs), their biological meaning, and key miRs potentially associated with rs2275888 eQTL in peripheral blood mononuclear leucocytes (PBML) of MI patients 6 months after first MI. Methods: Transcriptome data was obtained from PBMLs of 21 patients, who suffered ischemic MI, by employing Illumina iScan microarray technology. Genotyping for rs2275888 was conducted with real-time PCR, using TaqMan® assay. Preprocessing and identification of DEGs was done using limma package of R/Bioconductor software. The online tool DAVID v6.8 was employed for functional enrichment analysis. Most important miRs were selected using NetworkAnalyst web tool, based on the degree centrality value. Results: Transcriptome analysis in recessive model TT+TC (n=19) vs. CC (n=2) identified 68 DEGs. Top significant biological processes involving DEGs cover vascular physiology, cell growth and signaling. Pathway analysis associated DEGs with adherens junction, Rap1 and Ras signaling. Network analysis identified hsa-miR335-5p, -26b-5p, -93-5p, -16-5p, -124-3p, -20b-5p, -17-5p and -218-5p as miRs with top centrality degree in our DEGs list. Conclusion: Seven of eight detected miRs have already been described in MI pathology or suggested as potential biomarkers for MI. Our result suggest the importance of integration of eQTLs, biological processes and pathway analysis coupled with miR activity for further research in MI pathology.",
publisher = "Belgrade : University of Belgrade, Faculty of Biology",
journal = "CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts",
title = "Microarray transcriptome profiling in myocardial infarction regarding PTPLAD2 rs2275888 eQTL: a data scouting approach",
pages = "176-176",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12816"
}

Životić, I., Jovanović, I., Đurić, T., Stanković, A., Dekleva, M., Marković Nikolić, N., Alavantić, D.,& Živković, M.. (2017). Microarray transcriptome profiling in myocardial infarction regarding PTPLAD2 rs2275888 eQTL: a data scouting approach. in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts
Belgrade : University of Belgrade, Faculty of Biology., 176-176.
https://hdl.handle.net/21.15107/rcub_vinar_12816

Životić I, Jovanović I, Đurić T, Stanković A, Dekleva M, Marković Nikolić N, Alavantić D, Živković M. Microarray transcriptome profiling in myocardial infarction regarding PTPLAD2 rs2275888 eQTL: a data scouting approach. in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts. 2017;:176-176.
https://hdl.handle.net/21.15107/rcub_vinar_12816 .

Životić, Ivan, Jovanović, Ivan, Đurić, Tamara, Stanković, Aleksandra, Dekleva, Milica, Marković Nikolić, Nevena, Alavantić, Dragan, Živković, Maja, "Microarray transcriptome profiling in myocardial infarction regarding PTPLAD2 rs2275888 eQTL: a data scouting approach" in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts (2017):176-176,
https://hdl.handle.net/21.15107/rcub_vinar_12816 .

TY  - CONF
AU  - Đorđević, Ana
AU  - Živković, Maja
AU  - Dekleva, Milica
AU  - Marković-Nikolić, Nataša
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
AU  - Đurić, Tamara
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7177
C3  - Atherosclerosis
T1  - Snps in the Vicinity of Lgals-3 Locus and Different Shape-Types of Heart Remodeling After the First Myocardial Infarction: Preliminary Results
VL  - 263
SP  - E180
EP  - E180
DO  - 10.1016/j.atherosclerosis.2017.06.578
ER  - 

@conference{
author = "Đorđević, Ana and Živković, Maja and Dekleva, Milica and Marković-Nikolić, Nataša and Alavantić, Dragan and Stanković, Aleksandra and Đurić, Tamara",
year = "2017",
journal = "Atherosclerosis",
title = "Snps in the Vicinity of Lgals-3 Locus and Different Shape-Types of Heart Remodeling After the First Myocardial Infarction: Preliminary Results",
volume = "263",
pages = "E180-E180",
doi = "10.1016/j.atherosclerosis.2017.06.578"
}

Đorđević, A., Živković, M., Dekleva, M., Marković-Nikolić, N., Alavantić, D., Stanković, A.,& Đurić, T.. (2017). Snps in the Vicinity of Lgals-3 Locus and Different Shape-Types of Heart Remodeling After the First Myocardial Infarction: Preliminary Results. in Atherosclerosis, 263, E180-E180.
https://doi.org/10.1016/j.atherosclerosis.2017.06.578

Đorđević A, Živković M, Dekleva M, Marković-Nikolić N, Alavantić D, Stanković A, Đurić T. Snps in the Vicinity of Lgals-3 Locus and Different Shape-Types of Heart Remodeling After the First Myocardial Infarction: Preliminary Results. in Atherosclerosis. 2017;263:E180-E180.
doi:10.1016/j.atherosclerosis.2017.06.578 .

Đorđević, Ana, Živković, Maja, Dekleva, Milica, Marković-Nikolić, Nataša, Alavantić, Dragan, Stanković, Aleksandra, Đurić, Tamara, "Snps in the Vicinity of Lgals-3 Locus and Different Shape-Types of Heart Remodeling After the First Myocardial Infarction: Preliminary Results" in Atherosclerosis, 263 (2017):E180-E180,
https://doi.org/10.1016/j.atherosclerosis.2017.06.578 . .

TY  - CONF
AU  - Životić, Ivan
AU  - Živković, Maja
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Đorđević, Ana
AU  - Dekleva, Milica
AU  - Marković-Nikolić, Nevena
AU  - Alavantić, Dragan
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7178
AB  - Aim: Myocardial infarction (MI) is the clinical complication predominately
caused by coronary plaque buildup and rupture during the process of
atherosclerosis. In the 9p21 locus two important risk haplotype blocks
have been identified. The one that carries the risk for MI with the lead
variant rs10757278 and another characterized to influence progression of
the MI, with the lead variant rs518394. We have investigated association of
the two genetic variants with the ST-elevated MI in the gender specific
manner. We have also tested variants effect on p15 mRNA level as one of
the possible mechanisms of the variants effect.Methods: The study group included 147 patients (72 females) with angiographically assessed MI, and 240 healthy controls (90 females). DNA
and RNA (n¼28) where isolated from peripheral blood mono nuclear
leukocytes. Genotypes for rs10757278 A/G and rs518394 C/G, and relative
mRNA level for p15 were determined using commercial TaqMan® assays
on 7500 ABI Real-Time PCR.
Results: We have found significant association of rs10757278 GG with STelevated MI occurrence, with OR of 2.2 (CI¼1.07-4.5, p¼0.03) in females.
P15 mRNA was significantly down-regulated in G allele carriers (AG+GG vs
AA) by a mean factor of 0.449 (S.E. range is 0.188-1.059), p¼0.019 in the
whole group. The genetic variant rs518394 was not significantly associated
either with MI or p15 mRNA level.
Conclusions: Genotype GG of rs10757278 is significantly associated with
MI occurrence in females in Serbian population. On
C3  - Atherosclerosis
T1  - RS10757278 from 9P21 is associated with st-elevated myocardial infarction in females in population of Serbia
VL  - 263
SP  - e188
EP  - e188
DO  - 10.1016/j.atherosclerosis.2017.06.603
ER  - 

@conference{
author = "Životić, Ivan and Živković, Maja and Đurić, Tamara and Stanković, Aleksandra and Đorđević, Ana and Dekleva, Milica and Marković-Nikolić, Nevena and Alavantić, Dragan",
year = "2017",
abstract = "Aim: Myocardial infarction (MI) is the clinical complication predominately
caused by coronary plaque buildup and rupture during the process of
atherosclerosis. In the 9p21 locus two important risk haplotype blocks
have been identified. The one that carries the risk for MI with the lead
variant rs10757278 and another characterized to influence progression of
the MI, with the lead variant rs518394. We have investigated association of
the two genetic variants with the ST-elevated MI in the gender specific
manner. We have also tested variants effect on p15 mRNA level as one of
the possible mechanisms of the variants effect.Methods: The study group included 147 patients (72 females) with angiographically assessed MI, and 240 healthy controls (90 females). DNA
and RNA (n¼28) where isolated from peripheral blood mono nuclear
leukocytes. Genotypes for rs10757278 A/G and rs518394 C/G, and relative
mRNA level for p15 were determined using commercial TaqMan® assays
on 7500 ABI Real-Time PCR.
Results: We have found significant association of rs10757278 GG with STelevated MI occurrence, with OR of 2.2 (CI¼1.07-4.5, p¼0.03) in females.
P15 mRNA was significantly down-regulated in G allele carriers (AG+GG vs
AA) by a mean factor of 0.449 (S.E. range is 0.188-1.059), p¼0.019 in the
whole group. The genetic variant rs518394 was not significantly associated
either with MI or p15 mRNA level.
Conclusions: Genotype GG of rs10757278 is significantly associated with
MI occurrence in females in Serbian population. On",
journal = "Atherosclerosis",
title = "RS10757278 from 9P21 is associated with st-elevated myocardial infarction in females in population of Serbia",
volume = "263",
pages = "e188-e188",
doi = "10.1016/j.atherosclerosis.2017.06.603"
}

Životić, I., Živković, M., Đurić, T., Stanković, A., Đorđević, A., Dekleva, M., Marković-Nikolić, N.,& Alavantić, D.. (2017). RS10757278 from 9P21 is associated with st-elevated myocardial infarction in females in population of Serbia. in Atherosclerosis, 263, e188-e188.
https://doi.org/10.1016/j.atherosclerosis.2017.06.603

Životić I, Živković M, Đurić T, Stanković A, Đorđević A, Dekleva M, Marković-Nikolić N, Alavantić D. RS10757278 from 9P21 is associated with st-elevated myocardial infarction in females in population of Serbia. in Atherosclerosis. 2017;263:e188-e188.
doi:10.1016/j.atherosclerosis.2017.06.603 .

Životić, Ivan, Živković, Maja, Đurić, Tamara, Stanković, Aleksandra, Đorđević, Ana, Dekleva, Milica, Marković-Nikolić, Nevena, Alavantić, Dragan, "RS10757278 from 9P21 is associated with st-elevated myocardial infarction in females in population of Serbia" in Atherosclerosis, 263 (2017):e188-e188,
https://doi.org/10.1016/j.atherosclerosis.2017.06.603 . .

TY  - CONF
AU  - Dekleva, Milica
AU  - Đorđević, Ana
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Nikolic, N. M. Markovic
AU  - Đurić, Tamara
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1564
C3  - European Journal of Heart Failure
T1  - Heart failure development and rs2274273 in the vicinity of LGALS-3 locus, LGALS-3 relative mRNA expression in patients with first myocardial infarction
VL  - 19
IS  - SI
SP  - 552
EP  - 552
DO  - 10.1093/eurheartj/ehx493.P5296
ER  - 

@conference{
author = "Dekleva, Milica and Đorđević, Ana and Živković, Maja and Stanković, Aleksandra and Nikolic, N. M. Markovic and Đurić, Tamara",
year = "2017",
journal = "European Journal of Heart Failure",
title = "Heart failure development and rs2274273 in the vicinity of LGALS-3 locus, LGALS-3 relative mRNA expression in patients with first myocardial infarction",
volume = "19",
number = "SI",
pages = "552-552",
doi = "10.1093/eurheartj/ehx493.P5296"
}

Dekleva, M., Đorđević, A., Živković, M., Stanković, A., Nikolic, N. M. M.,& Đurić, T.. (2017). Heart failure development and rs2274273 in the vicinity of LGALS-3 locus, LGALS-3 relative mRNA expression in patients with first myocardial infarction. in European Journal of Heart Failure, 19(SI), 552-552.
https://doi.org/10.1093/eurheartj/ehx493.P5296

Dekleva M, Đorđević A, Živković M, Stanković A, Nikolic NMM, Đurić T. Heart failure development and rs2274273 in the vicinity of LGALS-3 locus, LGALS-3 relative mRNA expression in patients with first myocardial infarction. in European Journal of Heart Failure. 2017;19(SI):552-552.
doi:10.1093/eurheartj/ehx493.P5296 .

Dekleva, Milica, Đorđević, Ana, Živković, Maja, Stanković, Aleksandra, Nikolic, N. M. Markovic, Đurić, Tamara, "Heart failure development and rs2274273 in the vicinity of LGALS-3 locus, LGALS-3 relative mRNA expression in patients with first myocardial infarction" in European Journal of Heart Failure, 19, no. SI (2017):552-552,
https://doi.org/10.1093/eurheartj/ehx493.P5296 . .

TY  - CONF
AU  - Đorđević, Ana
AU  - Đurić, Tamara
AU  - Živković, Maja
AU  - Životić, Ivan
AU  - Dekleva, Milica
AU  - Marković-Nikolić, Nataša
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1101
C3  - European Journal of Heart Failure
T1  - Rs2274273 from LGALS-3 locus and cardiac ventricular remodeling after first myocardial infarction: preliminary results
VL  - 18
IS  - SI
SP  - 283
EP  - 284
UR  - https://hdl.handle.net/21.15107/rcub_vinar_1101
ER  - 

@conference{
author = "Đorđević, Ana and Đurić, Tamara and Živković, Maja and Životić, Ivan and Dekleva, Milica and Marković-Nikolić, Nataša and Alavantić, Dragan and Stanković, Aleksandra",
year = "2016",
journal = "European Journal of Heart Failure",
title = "Rs2274273 from LGALS-3 locus and cardiac ventricular remodeling after first myocardial infarction: preliminary results",
volume = "18",
number = "SI",
pages = "283-284",
url = "https://hdl.handle.net/21.15107/rcub_vinar_1101"
}

Đorđević, A., Đurić, T., Živković, M., Životić, I., Dekleva, M., Marković-Nikolić, N., Alavantić, D.,& Stanković, A.. (2016). Rs2274273 from LGALS-3 locus and cardiac ventricular remodeling after first myocardial infarction: preliminary results. in European Journal of Heart Failure, 18(SI), 283-284.
https://hdl.handle.net/21.15107/rcub_vinar_1101

Đorđević A, Đurić T, Živković M, Životić I, Dekleva M, Marković-Nikolić N, Alavantić D, Stanković A. Rs2274273 from LGALS-3 locus and cardiac ventricular remodeling after first myocardial infarction: preliminary results. in European Journal of Heart Failure. 2016;18(SI):283-284.
https://hdl.handle.net/21.15107/rcub_vinar_1101 .

Đorđević, Ana, Đurić, Tamara, Živković, Maja, Životić, Ivan, Dekleva, Milica, Marković-Nikolić, Nataša, Alavantić, Dragan, Stanković, Aleksandra, "Rs2274273 from LGALS-3 locus and cardiac ventricular remodeling after first myocardial infarction: preliminary results" in European Journal of Heart Failure, 18, no. SI (2016):283-284,
https://hdl.handle.net/21.15107/rcub_vinar_1101 .

TY  - CONF
AU  - Đorđević, Ana
AU  - Đurić, Tamara
AU  - Živković, Maja
AU  - Životić, Ivan
AU  - Dekleva, Milica
AU  - Marković-Nikolić, N.
AU  - Stanković, Aleksandra
AU  - Alavantić, Dragan
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12806
PB  - Belgrade : Serbian Physiological Society
C3  - 3rd Congress of physiological sciences of Serbia "Molecular, cellular and integrative basis of health and disease: Transdisciplinary approach" : Abstract book
T1  - RS2274273 gene polymorphisms in the locus that harbours LGALS-3 gene and left ventricular remodeling after first myocardial infarction: preliminary results
SP  - 109
EP  - 109
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12806
ER  - 

@conference{
author = "Đorđević, Ana and Đurić, Tamara and Živković, Maja and Životić, Ivan and Dekleva, Milica and Marković-Nikolić, N. and Stanković, Aleksandra and Alavantić, Dragan",
year = "2014",
publisher = "Belgrade : Serbian Physiological Society",
journal = "3rd Congress of physiological sciences of Serbia "Molecular, cellular and integrative basis of health and disease: Transdisciplinary approach" : Abstract book",
title = "RS2274273 gene polymorphisms in the locus that harbours LGALS-3 gene and left ventricular remodeling after first myocardial infarction: preliminary results",
pages = "109-109",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12806"
}

Đorđević, A., Đurić, T., Živković, M., Životić, I., Dekleva, M., Marković-Nikolić, N., Stanković, A.,& Alavantić, D.. (2014). RS2274273 gene polymorphisms in the locus that harbours LGALS-3 gene and left ventricular remodeling after first myocardial infarction: preliminary results. in 3rd Congress of physiological sciences of Serbia "Molecular, cellular and integrative basis of health and disease: Transdisciplinary approach" : Abstract book
Belgrade : Serbian Physiological Society., 109-109.
https://hdl.handle.net/21.15107/rcub_vinar_12806

Đorđević A, Đurić T, Živković M, Životić I, Dekleva M, Marković-Nikolić N, Stanković A, Alavantić D. RS2274273 gene polymorphisms in the locus that harbours LGALS-3 gene and left ventricular remodeling after first myocardial infarction: preliminary results. in 3rd Congress of physiological sciences of Serbia "Molecular, cellular and integrative basis of health and disease: Transdisciplinary approach" : Abstract book. 2014;:109-109.
https://hdl.handle.net/21.15107/rcub_vinar_12806 .

Đorđević, Ana, Đurić, Tamara, Živković, Maja, Životić, Ivan, Dekleva, Milica, Marković-Nikolić, N., Stanković, Aleksandra, Alavantić, Dragan, "RS2274273 gene polymorphisms in the locus that harbours LGALS-3 gene and left ventricular remodeling after first myocardial infarction: preliminary results" in 3rd Congress of physiological sciences of Serbia "Molecular, cellular and integrative basis of health and disease: Transdisciplinary approach" : Abstract book (2014):109-109,
https://hdl.handle.net/21.15107/rcub_vinar_12806 .

TY  - CONF
AU  - Đorđević, Ana
AU  - Živković, Maja
AU  - Dekleva, Milica
AU  - Marković-Nikolić, N.
AU  - Kuveljić, Jovana
AU  - Stanković, Aleksandra
AU  - Đurić, Tamara
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12807
PB  - Belgrade : Serbian Physiological Society
C3  - 3rd Congress of physiological sciences of Serbia "Molecular, cellular and integrative basis of health and disease: Transdisciplinary approach" : Abstract book
T1  - Matrix metalloproteinase-1 and matrix metalloproteinase-3 promoter gene polumorphisms in left ventricular remodeling after first myocardial infarction: preliminary results
SP  - 110
EP  - 110
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12807
ER  - 

@conference{
author = "Đorđević, Ana and Živković, Maja and Dekleva, Milica and Marković-Nikolić, N. and Kuveljić, Jovana and Stanković, Aleksandra and Đurić, Tamara",
year = "2014",
publisher = "Belgrade : Serbian Physiological Society",
journal = "3rd Congress of physiological sciences of Serbia "Molecular, cellular and integrative basis of health and disease: Transdisciplinary approach" : Abstract book",
title = "Matrix metalloproteinase-1 and matrix metalloproteinase-3 promoter gene polumorphisms in left ventricular remodeling after first myocardial infarction: preliminary results",
pages = "110-110",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12807"
}

Đorđević, A., Živković, M., Dekleva, M., Marković-Nikolić, N., Kuveljić, J., Stanković, A.,& Đurić, T.. (2014). Matrix metalloproteinase-1 and matrix metalloproteinase-3 promoter gene polumorphisms in left ventricular remodeling after first myocardial infarction: preliminary results. in 3rd Congress of physiological sciences of Serbia "Molecular, cellular and integrative basis of health and disease: Transdisciplinary approach" : Abstract book
Belgrade : Serbian Physiological Society., 110-110.
https://hdl.handle.net/21.15107/rcub_vinar_12807

Đorđević A, Živković M, Dekleva M, Marković-Nikolić N, Kuveljić J, Stanković A, Đurić T. Matrix metalloproteinase-1 and matrix metalloproteinase-3 promoter gene polumorphisms in left ventricular remodeling after first myocardial infarction: preliminary results. in 3rd Congress of physiological sciences of Serbia "Molecular, cellular and integrative basis of health and disease: Transdisciplinary approach" : Abstract book. 2014;:110-110.
https://hdl.handle.net/21.15107/rcub_vinar_12807 .

Đorđević, Ana, Živković, Maja, Dekleva, Milica, Marković-Nikolić, N., Kuveljić, Jovana, Stanković, Aleksandra, Đurić, Tamara, "Matrix metalloproteinase-1 and matrix metalloproteinase-3 promoter gene polumorphisms in left ventricular remodeling after first myocardial infarction: preliminary results" in 3rd Congress of physiological sciences of Serbia "Molecular, cellular and integrative basis of health and disease: Transdisciplinary approach" : Abstract book (2014):110-110,
https://hdl.handle.net/21.15107/rcub_vinar_12807 .

TY  - CONF
AU  - Životić, Ivan
AU  - Živković, Maja
AU  - Đurić, Tamara
AU  - Đorđević, Ana
AU  - Dekleva, Milica
AU  - Marković-Nikolić, N.
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12815
PB  - Belgrade : Serbian Physiological Society
C3  - 3rd Congress of physiological sciences of Serbia "Molecular, cellular and integrative basis of health and disease: Transdisciplinary approach" : Abstract book
T1  - Role of angiotensin-converting enzyme (ACE) I/D polymorphism in cardiac remodeling among patients with first myocardial infarction: preliminary results
SP  - 210
EP  - 210
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12815
ER  - 

@conference{
author = "Životić, Ivan and Živković, Maja and Đurić, Tamara and Đorđević, Ana and Dekleva, Milica and Marković-Nikolić, N. and Alavantić, Dragan and Stanković, Aleksandra",
year = "2014",
publisher = "Belgrade : Serbian Physiological Society",
journal = "3rd Congress of physiological sciences of Serbia "Molecular, cellular and integrative basis of health and disease: Transdisciplinary approach" : Abstract book",
title = "Role of angiotensin-converting enzyme (ACE) I/D polymorphism in cardiac remodeling among patients with first myocardial infarction: preliminary results",
pages = "210-210",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12815"
}

Životić, I., Živković, M., Đurić, T., Đorđević, A., Dekleva, M., Marković-Nikolić, N., Alavantić, D.,& Stanković, A.. (2014). Role of angiotensin-converting enzyme (ACE) I/D polymorphism in cardiac remodeling among patients with first myocardial infarction: preliminary results. in 3rd Congress of physiological sciences of Serbia "Molecular, cellular and integrative basis of health and disease: Transdisciplinary approach" : Abstract book
Belgrade : Serbian Physiological Society., 210-210.
https://hdl.handle.net/21.15107/rcub_vinar_12815

Životić I, Živković M, Đurić T, Đorđević A, Dekleva M, Marković-Nikolić N, Alavantić D, Stanković A. Role of angiotensin-converting enzyme (ACE) I/D polymorphism in cardiac remodeling among patients with first myocardial infarction: preliminary results. in 3rd Congress of physiological sciences of Serbia "Molecular, cellular and integrative basis of health and disease: Transdisciplinary approach" : Abstract book. 2014;:210-210.
https://hdl.handle.net/21.15107/rcub_vinar_12815 .

Životić, Ivan, Živković, Maja, Đurić, Tamara, Đorđević, Ana, Dekleva, Milica, Marković-Nikolić, N., Alavantić, Dragan, Stanković, Aleksandra, "Role of angiotensin-converting enzyme (ACE) I/D polymorphism in cardiac remodeling among patients with first myocardial infarction: preliminary results" in 3rd Congress of physiological sciences of Serbia "Molecular, cellular and integrative basis of health and disease: Transdisciplinary approach" : Abstract book (2014):210-210,
https://hdl.handle.net/21.15107/rcub_vinar_12815 .