TY  - JOUR
AU  - Stevanović, Magdalena
AU  - Savanovic, Igor
AU  - Uskokovic, Vuk
AU  - Škapin, Srečo Davor
AU  - Bracko, Ines
AU  - Jovanović, Uroš D.
AU  - Uskoković, Dragan
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4659
AB  - A simple and green chemical method has been developed to synthesize stable bare and capped silver nanoparticles based on the reduction of silver ions by glucose and capping by poly(alpha,gamma,l-glutamic acid) (PGA). The use of ammonia during synthesis was avoided. PGA has had a dual role in the synthesis and was used as a capping agent to make the silver nanoparticle more biocompatible and to protect the nanoparticles from agglomerating in the liquid medium. The synthesized PGA-capped silver nanoparticles in the size range 5-45 nm were stable over long periods of time, without signs of precipitation. Morphological examination has shown that the silver nanoparticles had a nearly spherical, multiply twinned structure. The effects of the reaction temperature and the reaction time during the synthesis were investigated too. The biocompatibility of the PGA-capped silver nanoparticles is discussed in terms of in vitro toxicity with human intestinal Caco-2 cells. The samples were characterized by UV-Visible spectroscopy, field emission scanning electron microscopy, transmission electron microscopy, and zeta potential measurements.
T2  - Colloid and Polymer Science
T1  - A new, simple, green, and one-pot four-component synthesis of bare and poly(alpha,gamma,l-glutamic acid)-capped silver nanoparticles
VL  - 290
IS  - 3
SP  - 221
EP  - 231
DO  - 10.1007/s00396-011-2540-7
ER  - 

@article{
author = "Stevanović, Magdalena and Savanovic, Igor and Uskokovic, Vuk and Škapin, Srečo Davor and Bracko, Ines and Jovanović, Uroš D. and Uskoković, Dragan",
year = "2012",
abstract = "A simple and green chemical method has been developed to synthesize stable bare and capped silver nanoparticles based on the reduction of silver ions by glucose and capping by poly(alpha,gamma,l-glutamic acid) (PGA). The use of ammonia during synthesis was avoided. PGA has had a dual role in the synthesis and was used as a capping agent to make the silver nanoparticle more biocompatible and to protect the nanoparticles from agglomerating in the liquid medium. The synthesized PGA-capped silver nanoparticles in the size range 5-45 nm were stable over long periods of time, without signs of precipitation. Morphological examination has shown that the silver nanoparticles had a nearly spherical, multiply twinned structure. The effects of the reaction temperature and the reaction time during the synthesis were investigated too. The biocompatibility of the PGA-capped silver nanoparticles is discussed in terms of in vitro toxicity with human intestinal Caco-2 cells. The samples were characterized by UV-Visible spectroscopy, field emission scanning electron microscopy, transmission electron microscopy, and zeta potential measurements.",
journal = "Colloid and Polymer Science",
title = "A new, simple, green, and one-pot four-component synthesis of bare and poly(alpha,gamma,l-glutamic acid)-capped silver nanoparticles",
volume = "290",
number = "3",
pages = "221-231",
doi = "10.1007/s00396-011-2540-7"
}

Stevanović, M., Savanovic, I., Uskokovic, V., Škapin, S. D., Bracko, I., Jovanović, U. D.,& Uskoković, D.. (2012). A new, simple, green, and one-pot four-component synthesis of bare and poly(alpha,gamma,l-glutamic acid)-capped silver nanoparticles. in Colloid and Polymer Science, 290(3), 221-231.
https://doi.org/10.1007/s00396-011-2540-7

Stevanović M, Savanovic I, Uskokovic V, Škapin SD, Bracko I, Jovanović UD, Uskoković D. A new, simple, green, and one-pot four-component synthesis of bare and poly(alpha,gamma,l-glutamic acid)-capped silver nanoparticles. in Colloid and Polymer Science. 2012;290(3):221-231.
doi:10.1007/s00396-011-2540-7 .

Stevanović, Magdalena, Savanovic, Igor, Uskokovic, Vuk, Škapin, Srečo Davor, Bracko, Ines, Jovanović, Uroš D., Uskoković, Dragan, "A new, simple, green, and one-pot four-component synthesis of bare and poly(alpha,gamma,l-glutamic acid)-capped silver nanoparticles" in Colloid and Polymer Science, 290, no. 3 (2012):221-231,
https://doi.org/10.1007/s00396-011-2540-7 . .

TY  - JOUR
AU  - Stevanović, Magdalena
AU  - Maksin, Tatjana N.
AU  - Petkovic, Jana
AU  - Filipic, Metka
AU  - Uskoković, Dragan
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3755
AB  - Nanoparticles of poly(DL-lactide-co-glycolide) (PLGA) in the size range 90-150 nm were produced using the physicochemical method with solvent/non-solvent systems. The encapsulation of the ascorbic acid in the polymer matrix was performed by homogenization of the water and organic phases. In vitro degradation and release tests of PLGA nanoparticles with and without encapsulated ascorbic acid were studied for more than 60 days in PBS and it has been determined that PLGA completely degrades within this period, fully releasing all encapsulated ascorbic acid. The cytotoxicity of PLGA and PLGA/ascorbic acid 85/15% nanoparticles was examined with human hepatoma cell lines (HepG2 ECACC), in vitro. The obtained results indicate that neither PLGA nanospheres nor PLGA/ascorbic acid 85/15% nanoparticles significantly affected the viability of the HepG2 cells. The investigation of the distribution and pharmacokinetics of PLGA is crucial for the effective prediction of host responses to PLGA in particular applications. Thus we present a method of labeling PLGA nanospheres and PLGA/ascorbic acid 85/15 wt% nanoparticles by (99m)Tc which binds outside, leaving the cage intact. This enables a quick and convenient investigation of the pharmacological behavior and metabolism of PLGA. The biodistribution of (99m)Tc-labeled PLGA particles with and without encapsulated ascorbic acid after different periods of time of their installation into rats was examined. PLGA nanospheres with encapsulated ascorbic acid exhibit prolonged blood circulation accompanied by time-dependent reduction in the lungs, liver and spleen, and addition in the kidney, stomach and intestine. The samples were characterized by x-ray diffraction, scanning electron microscopy, stereological analysis, transmission electron microscopy, ultraviolet spectroscopy and instant thin layer chromatography.
T2  - Nanotechnology
T1  - An innovative, quick and convenient labeling method for the investigation of pharmacological behavior and the metabolism of poly(DL-lactide-co-glycolide) nanospheres
VL  - 20
IS  - 33
DO  - 10.1088/0957-4484/20/33/335102
ER  - 

@article{
author = "Stevanović, Magdalena and Maksin, Tatjana N. and Petkovic, Jana and Filipic, Metka and Uskoković, Dragan",
year = "2009",
abstract = "Nanoparticles of poly(DL-lactide-co-glycolide) (PLGA) in the size range 90-150 nm were produced using the physicochemical method with solvent/non-solvent systems. The encapsulation of the ascorbic acid in the polymer matrix was performed by homogenization of the water and organic phases. In vitro degradation and release tests of PLGA nanoparticles with and without encapsulated ascorbic acid were studied for more than 60 days in PBS and it has been determined that PLGA completely degrades within this period, fully releasing all encapsulated ascorbic acid. The cytotoxicity of PLGA and PLGA/ascorbic acid 85/15% nanoparticles was examined with human hepatoma cell lines (HepG2 ECACC), in vitro. The obtained results indicate that neither PLGA nanospheres nor PLGA/ascorbic acid 85/15% nanoparticles significantly affected the viability of the HepG2 cells. The investigation of the distribution and pharmacokinetics of PLGA is crucial for the effective prediction of host responses to PLGA in particular applications. Thus we present a method of labeling PLGA nanospheres and PLGA/ascorbic acid 85/15 wt% nanoparticles by (99m)Tc which binds outside, leaving the cage intact. This enables a quick and convenient investigation of the pharmacological behavior and metabolism of PLGA. The biodistribution of (99m)Tc-labeled PLGA particles with and without encapsulated ascorbic acid after different periods of time of their installation into rats was examined. PLGA nanospheres with encapsulated ascorbic acid exhibit prolonged blood circulation accompanied by time-dependent reduction in the lungs, liver and spleen, and addition in the kidney, stomach and intestine. The samples were characterized by x-ray diffraction, scanning electron microscopy, stereological analysis, transmission electron microscopy, ultraviolet spectroscopy and instant thin layer chromatography.",
journal = "Nanotechnology",
title = "An innovative, quick and convenient labeling method for the investigation of pharmacological behavior and the metabolism of poly(DL-lactide-co-glycolide) nanospheres",
volume = "20",
number = "33",
doi = "10.1088/0957-4484/20/33/335102"
}

Stevanović, M., Maksin, T. N., Petkovic, J., Filipic, M.,& Uskoković, D.. (2009). An innovative, quick and convenient labeling method for the investigation of pharmacological behavior and the metabolism of poly(DL-lactide-co-glycolide) nanospheres. in Nanotechnology, 20(33).
https://doi.org/10.1088/0957-4484/20/33/335102

Stevanović M, Maksin TN, Petkovic J, Filipic M, Uskoković D. An innovative, quick and convenient labeling method for the investigation of pharmacological behavior and the metabolism of poly(DL-lactide-co-glycolide) nanospheres. in Nanotechnology. 2009;20(33).
doi:10.1088/0957-4484/20/33/335102 .

Stevanović, Magdalena, Maksin, Tatjana N., Petkovic, Jana, Filipic, Metka, Uskoković, Dragan, "An innovative, quick and convenient labeling method for the investigation of pharmacological behavior and the metabolism of poly(DL-lactide-co-glycolide) nanospheres" in Nanotechnology, 20, no. 33 (2009),
https://doi.org/10.1088/0957-4484/20/33/335102 . .

TY  - JOUR
AU  - Stevanović, Magdalena
AU  - Savić, Jasmina
AU  - Jordovic, Branka
AU  - Uskoković, Dragan
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3278
AB  - Ascorbic acid (vitamin Q is essential for preserving optimal health and is used by the body for many purposes. The problem is that ascorbic acid easily decomposes into biologically inactive compounds making its use very limited in the field of pharmaceuticals, dermatological and cosmetics. By encapsulating the ascorbic acid into a polymer matrix it is assumed that its chemical instability can be overcome as well as higher, more efficient and equally distributed concentration throughout extended period of time can be achieved. This paper is describing the process of obtaining poly(DL-lactide-co-glycolide) (DLPLG) nanospheres (110-170 nm) using chemical method with solvent/non-solvent systems where obtained solutions have been centrifuged. The encapsulation of the ascorbic acid in the polymer matrix is performed by homogenisation of water and organic phases. Nanoparticles of the copolymer DLPLG with the different contents of the ascorbic acid have different morphological characteristics, i.e. variable degree of uniformity, agglomeration, sizes and spherical shaping. The degradation of the nanospheres of DLPLG, DLPLG/ascorbic acid nanoparticles and release rate of the ascorbic acid were studied for 8 weeks in a physiological solution (0.9% sodium chloride in water). The samples have been characterised by infrared spectroscopy (IR), scanning electron microscopy (SEM), stereological analysis and ultraviolet (UV) spectroscopy. (C) 2007 Elsevier B.V. All rights reserved.
T2  - Colloids and Surfaces. B: Biointerfaces
T1  - Fabrication, in vitro degradation and the release behaviours of poly(DL-lactide-co-glycolide) nanospheres containing ascorbic acid
VL  - 59
IS  - 2
SP  - 215
EP  - 223
DO  - 10.1016/j.colsurfb.2007.05.011
ER  - 

@article{
author = "Stevanović, Magdalena and Savić, Jasmina and Jordovic, Branka and Uskoković, Dragan",
year = "2007",
abstract = "Ascorbic acid (vitamin Q is essential for preserving optimal health and is used by the body for many purposes. The problem is that ascorbic acid easily decomposes into biologically inactive compounds making its use very limited in the field of pharmaceuticals, dermatological and cosmetics. By encapsulating the ascorbic acid into a polymer matrix it is assumed that its chemical instability can be overcome as well as higher, more efficient and equally distributed concentration throughout extended period of time can be achieved. This paper is describing the process of obtaining poly(DL-lactide-co-glycolide) (DLPLG) nanospheres (110-170 nm) using chemical method with solvent/non-solvent systems where obtained solutions have been centrifuged. The encapsulation of the ascorbic acid in the polymer matrix is performed by homogenisation of water and organic phases. Nanoparticles of the copolymer DLPLG with the different contents of the ascorbic acid have different morphological characteristics, i.e. variable degree of uniformity, agglomeration, sizes and spherical shaping. The degradation of the nanospheres of DLPLG, DLPLG/ascorbic acid nanoparticles and release rate of the ascorbic acid were studied for 8 weeks in a physiological solution (0.9% sodium chloride in water). The samples have been characterised by infrared spectroscopy (IR), scanning electron microscopy (SEM), stereological analysis and ultraviolet (UV) spectroscopy. (C) 2007 Elsevier B.V. All rights reserved.",
journal = "Colloids and Surfaces. B: Biointerfaces",
title = "Fabrication, in vitro degradation and the release behaviours of poly(DL-lactide-co-glycolide) nanospheres containing ascorbic acid",
volume = "59",
number = "2",
pages = "215-223",
doi = "10.1016/j.colsurfb.2007.05.011"
}

Stevanović, M., Savić, J., Jordovic, B.,& Uskoković, D.. (2007). Fabrication, in vitro degradation and the release behaviours of poly(DL-lactide-co-glycolide) nanospheres containing ascorbic acid. in Colloids and Surfaces. B: Biointerfaces, 59(2), 215-223.
https://doi.org/10.1016/j.colsurfb.2007.05.011

Stevanović M, Savić J, Jordovic B, Uskoković D. Fabrication, in vitro degradation and the release behaviours of poly(DL-lactide-co-glycolide) nanospheres containing ascorbic acid. in Colloids and Surfaces. B: Biointerfaces. 2007;59(2):215-223.
doi:10.1016/j.colsurfb.2007.05.011 .

Stevanović, Magdalena, Savić, Jasmina, Jordovic, Branka, Uskoković, Dragan, "Fabrication, in vitro degradation and the release behaviours of poly(DL-lactide-co-glycolide) nanospheres containing ascorbic acid" in Colloids and Surfaces. B: Biointerfaces, 59, no. 2 (2007):215-223,
https://doi.org/10.1016/j.colsurfb.2007.05.011 . .

TY  - CONF
AU  - Stevanović, M.
AU  - Jordovic, B.
AU  - Nedić, Zoran
AU  - Miličević, Dejan S.
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6696
AB  - Copolymer poly(D,L-lactide-co-glycolide) is used for obtaining the systems for controlled delivery of medicaments. Its specific characteristics make it suitable for various researches where its synthesis is performed in different ways. Using the system for controlled delivery of medicaments, an equal concentration of the medicament is achieved in the body throughout an extended period of time and it has advantages over the conventional methods. In this paper we present a new solvent/non-solvent chemical method for obtaining DLPLG nanospheres. In the experiment various stabilizers were used in order to examine their influence on morphological characteristics of DLPLG particles. The samples were characterized by Infrared Spectroscopy (IR), Differential Scanning Calorimetry (DSC), Scanning Electron Microscopy (SEM) and Stereological analysis.
C3  - Materials Science Forum
T1  - The stabilizer influence on morphological characteristics of poly-(DL-lactide-co-glycolide) nanospheres
VL  - 555
SP  - 447
EP  - +
DO  - 10.4028/www.scientific.net/MSF.555.447
ER  - 

@conference{
author = "Stevanović, M. and Jordovic, B. and Nedić, Zoran and Miličević, Dejan S.",
year = "2007",
abstract = "Copolymer poly(D,L-lactide-co-glycolide) is used for obtaining the systems for controlled delivery of medicaments. Its specific characteristics make it suitable for various researches where its synthesis is performed in different ways. Using the system for controlled delivery of medicaments, an equal concentration of the medicament is achieved in the body throughout an extended period of time and it has advantages over the conventional methods. In this paper we present a new solvent/non-solvent chemical method for obtaining DLPLG nanospheres. In the experiment various stabilizers were used in order to examine their influence on morphological characteristics of DLPLG particles. The samples were characterized by Infrared Spectroscopy (IR), Differential Scanning Calorimetry (DSC), Scanning Electron Microscopy (SEM) and Stereological analysis.",
journal = "Materials Science Forum",
title = "The stabilizer influence on morphological characteristics of poly-(DL-lactide-co-glycolide) nanospheres",
volume = "555",
pages = "447-+",
doi = "10.4028/www.scientific.net/MSF.555.447"
}

Stevanović, M., Jordovic, B., Nedić, Z.,& Miličević, D. S.. (2007). The stabilizer influence on morphological characteristics of poly-(DL-lactide-co-glycolide) nanospheres. in Materials Science Forum, 555, 447-+.
https://doi.org/10.4028/www.scientific.net/MSF.555.447

Stevanović M, Jordovic B, Nedić Z, Miličević DS. The stabilizer influence on morphological characteristics of poly-(DL-lactide-co-glycolide) nanospheres. in Materials Science Forum. 2007;555:447-+.
doi:10.4028/www.scientific.net/MSF.555.447 .

Stevanović, M., Jordovic, B., Nedić, Zoran, Miličević, Dejan S., "The stabilizer influence on morphological characteristics of poly-(DL-lactide-co-glycolide) nanospheres" in Materials Science Forum, 555 (2007):447-+,
https://doi.org/10.4028/www.scientific.net/MSF.555.447 . .