TY  - JOUR
AU  - Đurašević, Siniša
AU  - Pejić, Snežana
AU  - Grigorov, Ilijana
AU  - Nikolić, Gorana
AU  - Mitić-Ćulafić, Dragana
AU  - Dragićević, Milan
AU  - Đorđević, Jelena
AU  - Todorović Vukotić, Nevena
AU  - Đorđević, Neda O.
AU  - Todorović, Ana
AU  - Drakulić, Dunja R.
AU  - Veljković, Filip M.
AU  - Pajović, Snežana B.
AU  - Todorović, Zoran
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9831
AB  - Thioacetamide (TAA) is widely used to study liver toxicity accompanied by oxidative stress, inflammation, cell necrosis, fibrosis, cholestasis, and hepatocellular carcinoma. As an efficient free radical’s scavenger, C60 fullerene is considered a potential liver-protective agent in chemically-induced liver injury. In the present work, we examined the hepatoprotective effects of two C60 doses dissolved in virgin olive oil against TAA-induced hepatotoxicity in rats. We showed that TAA-induced increase in liver oxidative stress, judged by the changes in the activities of SOD, CAT, GPx, GR, GST, the content of GSH and 4-HNE, and expression of HO-1, MnSOD, and CuZnSOD, was more effectively ameliorated with a lower C60 dose. Improvement in liver antioxidative status caused by C60 was accompanied by a decrease in liver HMGB1 expression and an increase in nuclear Nrf2/NF-κB p65 ratio, suggesting a reduction in inflammation, necrosis and fibrosis. These results were in accordance with liver histology analysis, liver comet assay, and changes in serum levels of ALT, AST, and AP. The changes observed in gut microbiome support detrimental effects of TAA and hepatoprotective effects of low C60 dose. Less protective effects of a higher C60 dose could be a consequence of its enhanced aggregation and related pro-oxidant role.
T2  - Antioxidants
T1  - Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes
VL  - 10
IS  - 6
SP  - 911
DO  - 10.3390/antiox10060911
ER  - 

@article{
author = "Đurašević, Siniša and Pejić, Snežana and Grigorov, Ilijana and Nikolić, Gorana and Mitić-Ćulafić, Dragana and Dragićević, Milan and Đorđević, Jelena and Todorović Vukotić, Nevena and Đorđević, Neda O. and Todorović, Ana and Drakulić, Dunja R. and Veljković, Filip M. and Pajović, Snežana B. and Todorović, Zoran",
year = "2021",
abstract = "Thioacetamide (TAA) is widely used to study liver toxicity accompanied by oxidative stress, inflammation, cell necrosis, fibrosis, cholestasis, and hepatocellular carcinoma. As an efficient free radical’s scavenger, C60 fullerene is considered a potential liver-protective agent in chemically-induced liver injury. In the present work, we examined the hepatoprotective effects of two C60 doses dissolved in virgin olive oil against TAA-induced hepatotoxicity in rats. We showed that TAA-induced increase in liver oxidative stress, judged by the changes in the activities of SOD, CAT, GPx, GR, GST, the content of GSH and 4-HNE, and expression of HO-1, MnSOD, and CuZnSOD, was more effectively ameliorated with a lower C60 dose. Improvement in liver antioxidative status caused by C60 was accompanied by a decrease in liver HMGB1 expression and an increase in nuclear Nrf2/NF-κB p65 ratio, suggesting a reduction in inflammation, necrosis and fibrosis. These results were in accordance with liver histology analysis, liver comet assay, and changes in serum levels of ALT, AST, and AP. The changes observed in gut microbiome support detrimental effects of TAA and hepatoprotective effects of low C60 dose. Less protective effects of a higher C60 dose could be a consequence of its enhanced aggregation and related pro-oxidant role.",
journal = "Antioxidants",
title = "Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes",
volume = "10",
number = "6",
pages = "911",
doi = "10.3390/antiox10060911"
}

Đurašević, S., Pejić, S., Grigorov, I., Nikolić, G., Mitić-Ćulafić, D., Dragićević, M., Đorđević, J., Todorović Vukotić, N., Đorđević, N. O., Todorović, A., Drakulić, D. R., Veljković, F. M., Pajović, S. B.,& Todorović, Z.. (2021). Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes. in Antioxidants, 10(6), 911.
https://doi.org/10.3390/antiox10060911

Đurašević S, Pejić S, Grigorov I, Nikolić G, Mitić-Ćulafić D, Dragićević M, Đorđević J, Todorović Vukotić N, Đorđević NO, Todorović A, Drakulić DR, Veljković FM, Pajović SB, Todorović Z. Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes. in Antioxidants. 2021;10(6):911.
doi:10.3390/antiox10060911 .

Đurašević, Siniša, Pejić, Snežana, Grigorov, Ilijana, Nikolić, Gorana, Mitić-Ćulafić, Dragana, Dragićević, Milan, Đorđević, Jelena, Todorović Vukotić, Nevena, Đorđević, Neda O., Todorović, Ana, Drakulić, Dunja R., Veljković, Filip M., Pajović, Snežana B., Todorović, Zoran, "Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes" in Antioxidants, 10, no. 6 (2021):911,
https://doi.org/10.3390/antiox10060911 . .

TY  - JOUR
AU  - Đurašević, Siniša
AU  - Nikolić, Gorana V.
AU  - Todorović, Ana
AU  - Drakulić, Dunja R.
AU  - Pejić, Snežana
AU  - Martinović, Vesna
AU  - Mitić-Ćulafić, Dragana
AU  - Milić, Dragana
AU  - Kop, Tatjana
AU  - Jasnić, Nebojša
AU  - Đorđević, Jelena D.
AU  - Todorović, Zoran
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8924
AB  - The effects of twelve weeks of supplementation with fullerene C60 olive/coconut oil solution on a broad spectrum of parameters in rats were examined. The tissue bioaccumulation of C60 was shown to be tissue-specific, with the liver, heart, and adrenal glands being the organs of the greatest, and the kidney, brain, and spleen being the organs of the smallest accumulation. C60 did not change aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase serum activities level, nor the damage of liver cells DNA. There were no effects of fullerene on prooxidant-antioxidant balance in the liver, kidney, spleen, heart, and brain, nor any visible harmful effects on the liver, heart, aorta, spleen, kidney, and small intestine histology. Fullerene changed the gut microbiota structure towards the bacteria that ameliorate lipid homeostasis, causing a serum triglycerides concentration decrease. However, C60 significantly increased the insulin resistance, serum ascorbate oxidation, and brain malondialdehyde and advanced oxidation protein products level. The deteriorative effects of C60 on the brain and serum could be attributed to the specific physicochemical composition of these tissues, potentiating the C60 aggregation or biotransformation as the key element of its pro-oxidative action.
T2  - Food and Chemical Toxicology
T1  - Effects of fullerene C60 supplementation on gut microbiota and glucose and lipid homeostasis in rats
VL  - 140
DO  - 10.1016/j.fct.2020.111302
ER  - 

@article{
author = "Đurašević, Siniša and Nikolić, Gorana V. and Todorović, Ana and Drakulić, Dunja R. and Pejić, Snežana and Martinović, Vesna and Mitić-Ćulafić, Dragana and Milić, Dragana and Kop, Tatjana and Jasnić, Nebojša and Đorđević, Jelena D. and Todorović, Zoran",
year = "2020",
abstract = "The effects of twelve weeks of supplementation with fullerene C60 olive/coconut oil solution on a broad spectrum of parameters in rats were examined. The tissue bioaccumulation of C60 was shown to be tissue-specific, with the liver, heart, and adrenal glands being the organs of the greatest, and the kidney, brain, and spleen being the organs of the smallest accumulation. C60 did not change aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase serum activities level, nor the damage of liver cells DNA. There were no effects of fullerene on prooxidant-antioxidant balance in the liver, kidney, spleen, heart, and brain, nor any visible harmful effects on the liver, heart, aorta, spleen, kidney, and small intestine histology. Fullerene changed the gut microbiota structure towards the bacteria that ameliorate lipid homeostasis, causing a serum triglycerides concentration decrease. However, C60 significantly increased the insulin resistance, serum ascorbate oxidation, and brain malondialdehyde and advanced oxidation protein products level. The deteriorative effects of C60 on the brain and serum could be attributed to the specific physicochemical composition of these tissues, potentiating the C60 aggregation or biotransformation as the key element of its pro-oxidative action.",
journal = "Food and Chemical Toxicology",
title = "Effects of fullerene C60 supplementation on gut microbiota and glucose and lipid homeostasis in rats",
volume = "140",
doi = "10.1016/j.fct.2020.111302"
}

Đurašević, S., Nikolić, G. V., Todorović, A., Drakulić, D. R., Pejić, S., Martinović, V., Mitić-Ćulafić, D., Milić, D., Kop, T., Jasnić, N., Đorđević, J. D.,& Todorović, Z.. (2020). Effects of fullerene C60 supplementation on gut microbiota and glucose and lipid homeostasis in rats. in Food and Chemical Toxicology, 140.
https://doi.org/10.1016/j.fct.2020.111302

Đurašević S, Nikolić GV, Todorović A, Drakulić DR, Pejić S, Martinović V, Mitić-Ćulafić D, Milić D, Kop T, Jasnić N, Đorđević JD, Todorović Z. Effects of fullerene C60 supplementation on gut microbiota and glucose and lipid homeostasis in rats. in Food and Chemical Toxicology. 2020;140.
doi:10.1016/j.fct.2020.111302 .

Đurašević, Siniša, Nikolić, Gorana V., Todorović, Ana, Drakulić, Dunja R., Pejić, Snežana, Martinović, Vesna, Mitić-Ćulafić, Dragana, Milić, Dragana, Kop, Tatjana, Jasnić, Nebojša, Đorđević, Jelena D., Todorović, Zoran, "Effects of fullerene C60 supplementation on gut microbiota and glucose and lipid homeostasis in rats" in Food and Chemical Toxicology, 140 (2020),
https://doi.org/10.1016/j.fct.2020.111302 . .

TY  - CONF
AU  - Cvetković, Stefana
AU  - Đukanović, S.
AU  - Mitić-Ćulafić, Dragana
AU  - Nastasijević, Branislav J.
AU  - Knežević-Vukčević, Jelena
AU  - Nikolić, Biljana
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8627
AB  - During high-temperature cooking of protein rich foods, especially meat and fish, heterocyclic aromatic amines can be formed. These amines are a class of potent mutagens that can cause alterations in the structure of DNA and chromosomes. In recent decades, research has been focused on investigating plants and their phytochemicals as potential antimutagens. The aim of this study was to examine the anti-genotoxic effect of methanolic root and leaf extracts of Gentiana lutea against the food mutagens 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) produced in thermally processed meat. To determine the protective potential of extracts, the alkaline comet assay was applied. The results obtained indicated strong anti-genotoxic effect of both extracts against the tested mutagens. The highest inhibition of IQ-induced genotoxicity was recorded for leaf extract (72%). Regarding PhiP, root extract achieved inhibition of 80% of DNA damage, so was more successful than leaf extract. The data obtained in this study stimulates further research of G. lutea extracts and its constituents as potential dietary supplements in improving human health. © Published under licence by IOP Publishing Ltd.
C3  - IOP Conference Series: Earth and Environmental Science
T1  - Protective effect of Gentiana lutea root and leaf extracts against heterocyclic aromatic amines IQ and PhIP produced in thermally processed meat
VL  - 333
IS  - 1
SP  - UNSP 012052
DO  - 10.1088/1755-1315/333/1/012052
ER  - 

@conference{
author = "Cvetković, Stefana and Đukanović, S. and Mitić-Ćulafić, Dragana and Nastasijević, Branislav J. and Knežević-Vukčević, Jelena and Nikolić, Biljana",
year = "2019",
abstract = "During high-temperature cooking of protein rich foods, especially meat and fish, heterocyclic aromatic amines can be formed. These amines are a class of potent mutagens that can cause alterations in the structure of DNA and chromosomes. In recent decades, research has been focused on investigating plants and their phytochemicals as potential antimutagens. The aim of this study was to examine the anti-genotoxic effect of methanolic root and leaf extracts of Gentiana lutea against the food mutagens 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) produced in thermally processed meat. To determine the protective potential of extracts, the alkaline comet assay was applied. The results obtained indicated strong anti-genotoxic effect of both extracts against the tested mutagens. The highest inhibition of IQ-induced genotoxicity was recorded for leaf extract (72%). Regarding PhiP, root extract achieved inhibition of 80% of DNA damage, so was more successful than leaf extract. The data obtained in this study stimulates further research of G. lutea extracts and its constituents as potential dietary supplements in improving human health. © Published under licence by IOP Publishing Ltd.",
journal = "IOP Conference Series: Earth and Environmental Science",
title = "Protective effect of Gentiana lutea root and leaf extracts against heterocyclic aromatic amines IQ and PhIP produced in thermally processed meat",
volume = "333",
number = "1",
pages = "UNSP 012052",
doi = "10.1088/1755-1315/333/1/012052"
}

Cvetković, S., Đukanović, S., Mitić-Ćulafić, D., Nastasijević, B. J., Knežević-Vukčević, J.,& Nikolić, B.. (2019). Protective effect of Gentiana lutea root and leaf extracts against heterocyclic aromatic amines IQ and PhIP produced in thermally processed meat. in IOP Conference Series: Earth and Environmental Science, 333(1), UNSP 012052.
https://doi.org/10.1088/1755-1315/333/1/012052

Cvetković S, Đukanović S, Mitić-Ćulafić D, Nastasijević BJ, Knežević-Vukčević J, Nikolić B. Protective effect of Gentiana lutea root and leaf extracts against heterocyclic aromatic amines IQ and PhIP produced in thermally processed meat. in IOP Conference Series: Earth and Environmental Science. 2019;333(1):UNSP 012052.
doi:10.1088/1755-1315/333/1/012052 .

Cvetković, Stefana, Đukanović, S., Mitić-Ćulafić, Dragana, Nastasijević, Branislav J., Knežević-Vukčević, Jelena, Nikolić, Biljana, "Protective effect of Gentiana lutea root and leaf extracts against heterocyclic aromatic amines IQ and PhIP produced in thermally processed meat" in IOP Conference Series: Earth and Environmental Science, 333, no. 1 (2019):UNSP 012052,
https://doi.org/10.1088/1755-1315/333/1/012052 . .

TY  - JOUR
AU  - Manojlović, Dragica
AU  - Dramićanin, Miroslav
AU  - Miletić, Vesna
AU  - Mitić-Ćulafić, Dragana
AU  - Jovanović, Bojana
AU  - Nikolić, Biljana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1462
AB  - Objective. To compare cytotoxicity and genotoxicity of novel urethane-based monomer FIT-852 and monoacylphosphine oxide photoinitiator (Lucirin TPO) with conventional Bisphenol A-glycidyl-methacrylate (BisGMA) and triethylene glycol dimethacrylate (TEGDMA) monomers and camphorquinone (CQ)/amine photoinitiator system, respectively. Moreover, we quantified and analyzed the combinatorial effects of individual substances in resin-based mixtures concerning the nature of the combinatorial effects. Methods. Cytotoxic and genotoxic effects of BisGMA, FIT, TEGDMA, CQ DMAEMA and TPO and their combined toxicity in four clinically relevant mixtures (FIT/TPO, FIT/CQ BisGMA/TPO, BisGMA/CQ) were tested on human fetal lung fibroblasts MRC-5 using MTT and Comet assays. We assessed combination effects of monomers and photoinitiators on overall toxicity from the measured concentration-effect relationships. Combination index (CI) was calculated on the basis of the median-effect equation derived from the mass-action law principle. Results. Individual substances showed decreasing cytotoxic effects in the following order: BisGMA GT TPO GT FIT GT CQ GT DMAEMA GT TEGDMA. Experimental mixtures showed decreasing cytotoxic effects in the order BisGMA/TPO GT BisGMA/CQ GT FIT/CQ GT FIT/TPO. FIT-based mixtures exhibited antagonistic cytotoxic effects between components while BisGMA-based mixtures demonstrated synergistic effects at ED50 TPO amplified both antagonistic and synergistic cytotoxic effects in mixtures. Pure substances showed genotoxicity in the following order: TPO GT BisGMA GT FIT GT CQ GT TEGDMA. We did not detect the genotoxic potential of DMAEMA. The rank of genotoxic concentrations of the mixtures was: BisGMA/TPO GT BisGMA/CQ GT FIT/CQ GT FIT/TPO. Significance. Lower cytotoxicity and genotoxicity of FIT than BisGMA suggests its greater bio-co mpatibility. Conversely, photoinitiator TPO was significantly more cytotoxic and genotoxic than both CQ and DMAEMA. CI values showed that components of FIT-based mixtures exhibit an antagonistic cytotoxic effect, while compontents of BisGMA-based mixtures show synergism.
T2  - Dental Materials
T1  - Cytotoxicity and genotoxicity of a low-shrinkage monomer and monoacylphosphine oxide photoinitiator: Comparative analyses of individual toxicity and combination effects in mixtures
VL  - 33
IS  - 4
SP  - 454
EP  - 466
DO  - 10.1016/j.dental.2017.02.002
ER  - 

@article{
author = "Manojlović, Dragica and Dramićanin, Miroslav and Miletić, Vesna and Mitić-Ćulafić, Dragana and Jovanović, Bojana and Nikolić, Biljana",
year = "2017",
abstract = "Objective. To compare cytotoxicity and genotoxicity of novel urethane-based monomer FIT-852 and monoacylphosphine oxide photoinitiator (Lucirin TPO) with conventional Bisphenol A-glycidyl-methacrylate (BisGMA) and triethylene glycol dimethacrylate (TEGDMA) monomers and camphorquinone (CQ)/amine photoinitiator system, respectively. Moreover, we quantified and analyzed the combinatorial effects of individual substances in resin-based mixtures concerning the nature of the combinatorial effects. Methods. Cytotoxic and genotoxic effects of BisGMA, FIT, TEGDMA, CQ DMAEMA and TPO and their combined toxicity in four clinically relevant mixtures (FIT/TPO, FIT/CQ BisGMA/TPO, BisGMA/CQ) were tested on human fetal lung fibroblasts MRC-5 using MTT and Comet assays. We assessed combination effects of monomers and photoinitiators on overall toxicity from the measured concentration-effect relationships. Combination index (CI) was calculated on the basis of the median-effect equation derived from the mass-action law principle. Results. Individual substances showed decreasing cytotoxic effects in the following order: BisGMA GT TPO GT FIT GT CQ GT DMAEMA GT TEGDMA. Experimental mixtures showed decreasing cytotoxic effects in the order BisGMA/TPO GT BisGMA/CQ GT FIT/CQ GT FIT/TPO. FIT-based mixtures exhibited antagonistic cytotoxic effects between components while BisGMA-based mixtures demonstrated synergistic effects at ED50 TPO amplified both antagonistic and synergistic cytotoxic effects in mixtures. Pure substances showed genotoxicity in the following order: TPO GT BisGMA GT FIT GT CQ GT TEGDMA. We did not detect the genotoxic potential of DMAEMA. The rank of genotoxic concentrations of the mixtures was: BisGMA/TPO GT BisGMA/CQ GT FIT/CQ GT FIT/TPO. Significance. Lower cytotoxicity and genotoxicity of FIT than BisGMA suggests its greater bio-co mpatibility. Conversely, photoinitiator TPO was significantly more cytotoxic and genotoxic than both CQ and DMAEMA. CI values showed that components of FIT-based mixtures exhibit an antagonistic cytotoxic effect, while compontents of BisGMA-based mixtures show synergism.",
journal = "Dental Materials",
title = "Cytotoxicity and genotoxicity of a low-shrinkage monomer and monoacylphosphine oxide photoinitiator: Comparative analyses of individual toxicity and combination effects in mixtures",
volume = "33",
number = "4",
pages = "454-466",
doi = "10.1016/j.dental.2017.02.002"
}

Manojlović, D., Dramićanin, M., Miletić, V., Mitić-Ćulafić, D., Jovanović, B.,& Nikolić, B.. (2017). Cytotoxicity and genotoxicity of a low-shrinkage monomer and monoacylphosphine oxide photoinitiator: Comparative analyses of individual toxicity and combination effects in mixtures. in Dental Materials, 33(4), 454-466.
https://doi.org/10.1016/j.dental.2017.02.002

Manojlović D, Dramićanin M, Miletić V, Mitić-Ćulafić D, Jovanović B, Nikolić B. Cytotoxicity and genotoxicity of a low-shrinkage monomer and monoacylphosphine oxide photoinitiator: Comparative analyses of individual toxicity and combination effects in mixtures. in Dental Materials. 2017;33(4):454-466.
doi:10.1016/j.dental.2017.02.002 .

Manojlović, Dragica, Dramićanin, Miroslav, Miletić, Vesna, Mitić-Ćulafić, Dragana, Jovanović, Bojana, Nikolić, Biljana, "Cytotoxicity and genotoxicity of a low-shrinkage monomer and monoacylphosphine oxide photoinitiator: Comparative analyses of individual toxicity and combination effects in mixtures" in Dental Materials, 33, no. 4 (2017):454-466,
https://doi.org/10.1016/j.dental.2017.02.002 . .

TY  - JOUR
AU  - Kuzmanović, Maja D.
AU  - Božanić, Dušan K.
AU  - Milivojević, Dušan
AU  - Mitić-Ćulafić, Dragana
AU  - Stanković, Slaviša
AU  - Ballesteros, Carmen
AU  - Gonzalez-Benito, Javier
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1837
AB  - Manganese-doped cadmium sulfide (CdS:Mn) nanoparticles were prepared by chemical synthesis using sodium-alginate as template. The preparation of the nanocomposites involved ionic crosslinking of the biopolymer by dimerization of its alpha-L-guluronic monomers with Cd2+ and subsequent formation of the semiconductor nanoparticles upon addition of sulfide ions in the presence of Mn2+. The crystalline phase of CdS in the material was confirmed by XRD. The surface morphology of the nanocomposites was investigated by SEM. The observation by TEM showed that the CdS:Mn particles were spherical in shape with diameters of approximately 4 nm. EPR measurements of the CdS:Mn-alginate nanocomposite showed that the Mn2+ ions were incorporated in cationic sites of CdS with lower symmetry. Due to a distorted crystal field induced by the Mn2+ ions, photoluminescence spectra of the CdS:Mn-alginate showed red fluorescence between 650 nm and 750 nm falling into the optical window for bioimaging in which the light has its maximum tissue penetration depth. It was demonstrated that the interaction between the nanoparticles and the matrix prevents release of CdS into the environment, leading to low acute toxicity of the nanocomposites.
T2  - RSC Advances
T1  - Sodium-alginate biopolymer as a template for the synthesis of nontoxic red emitting Mn2+-doped CdS nanoparticles
VL  - 7
IS  - 84
SP  - 53422
EP  - 53432
DO  - 10.1039/c7ra11011a
ER  - 

@article{
author = "Kuzmanović, Maja D. and Božanić, Dušan K. and Milivojević, Dušan and Mitić-Ćulafić, Dragana and Stanković, Slaviša and Ballesteros, Carmen and Gonzalez-Benito, Javier",
year = "2017",
abstract = "Manganese-doped cadmium sulfide (CdS:Mn) nanoparticles were prepared by chemical synthesis using sodium-alginate as template. The preparation of the nanocomposites involved ionic crosslinking of the biopolymer by dimerization of its alpha-L-guluronic monomers with Cd2+ and subsequent formation of the semiconductor nanoparticles upon addition of sulfide ions in the presence of Mn2+. The crystalline phase of CdS in the material was confirmed by XRD. The surface morphology of the nanocomposites was investigated by SEM. The observation by TEM showed that the CdS:Mn particles were spherical in shape with diameters of approximately 4 nm. EPR measurements of the CdS:Mn-alginate nanocomposite showed that the Mn2+ ions were incorporated in cationic sites of CdS with lower symmetry. Due to a distorted crystal field induced by the Mn2+ ions, photoluminescence spectra of the CdS:Mn-alginate showed red fluorescence between 650 nm and 750 nm falling into the optical window for bioimaging in which the light has its maximum tissue penetration depth. It was demonstrated that the interaction between the nanoparticles and the matrix prevents release of CdS into the environment, leading to low acute toxicity of the nanocomposites.",
journal = "RSC Advances",
title = "Sodium-alginate biopolymer as a template for the synthesis of nontoxic red emitting Mn2+-doped CdS nanoparticles",
volume = "7",
number = "84",
pages = "53422-53432",
doi = "10.1039/c7ra11011a"
}

Kuzmanović, M. D., Božanić, D. K., Milivojević, D., Mitić-Ćulafić, D., Stanković, S., Ballesteros, C.,& Gonzalez-Benito, J.. (2017). Sodium-alginate biopolymer as a template for the synthesis of nontoxic red emitting Mn2+-doped CdS nanoparticles. in RSC Advances, 7(84), 53422-53432.
https://doi.org/10.1039/c7ra11011a

Kuzmanović MD, Božanić DK, Milivojević D, Mitić-Ćulafić D, Stanković S, Ballesteros C, Gonzalez-Benito J. Sodium-alginate biopolymer as a template for the synthesis of nontoxic red emitting Mn2+-doped CdS nanoparticles. in RSC Advances. 2017;7(84):53422-53432.
doi:10.1039/c7ra11011a .

Kuzmanović, Maja D., Božanić, Dušan K., Milivojević, Dušan, Mitić-Ćulafić, Dragana, Stanković, Slaviša, Ballesteros, Carmen, Gonzalez-Benito, Javier, "Sodium-alginate biopolymer as a template for the synthesis of nontoxic red emitting Mn2+-doped CdS nanoparticles" in RSC Advances, 7, no. 84 (2017):53422-53432,
https://doi.org/10.1039/c7ra11011a . .

TY  - JOUR
AU  - Petrović, V.
AU  - Opacic-Galic, V.
AU  - Zivkovic, S.
AU  - Nikolić, Biljana
AU  - Danilovic, V.
AU  - Miletic, V.
AU  - Jokanović, Vukoman R.
AU  - Mitić-Ćulafić, Dragana
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/722
AB  - AimTo evaluate in vitro cytotoxicity and in vivo inflammatory response to new nanostructural materials based on active calcium silicate systems (CS) and hydroxyapatite (HA-CS). MethodologyCytotoxicity of eluates of new nanostructural noncommercial materials CS and HA-CS, and MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil) as a control, were tested using the MTT assay on MRC-5 cells. Eluates of set materials were tested in 100% and 50% concentrations, 24h, 7days and 21days post-elution. The pH values were determined for undiluted eluates of set materials. Polyethylene tubes containing the test materials (CS, HA-CS, MTA) were implanted in subcutaneous tissue of Wistar rats. Histopathological examinations were conducted at 7, 15, 30 and 60days after the implantation. Data were statistically analyzed using three-way and one-way anova Tukeys post hoc test as well as Kruskall-Wallis test with Dunns post hoc test at =0.05. ResultsAll materials significantly reduced cell viability; especially when undiluted eluates were used (P LT 0.001). After 24h elution, cell viability was 101.8%, 49.5 +/- 4.2% and 61 +/- 7.4%, for MTA, and HA-CS, respectively. However, CS and HA-CS were significantly less toxic than the control material MTA (P LT 0.05). Cytotoxicity could be at least partially attributed to pH kinetics over time. Dilution of eluates of all tested materials resulted in better cell survival. Histopathological examination indicated similar inflammatory reaction, vascular congestion and connective tissue integrity associated with CS, HA-CS and MTA at each observation period (P GT 0.05). The only significant difference was found for capsule thickness, that is thicker capsule was associated with HA-CS compared to MTA at 60days (P=0.0039). HA-CS induced moderately thick capsules (median score 3, score range 2-3), whereas MTA resulted in thin capsule formation (median score 2, score range 1-3). ConclusionsEvaluation of cytotoxicity and inflammatory response indicated better biocompatibility of CS and HA-CS, in comparison with MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil).
T2  - International Endodontic Journal
T1  - Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study
VL  - 48
IS  - 10
SP  - 966
EP  - 975
DO  - 10.1111/iej.12391
ER  - 

@article{
author = "Petrović, V. and Opacic-Galic, V. and Zivkovic, S. and Nikolić, Biljana and Danilovic, V. and Miletic, V. and Jokanović, Vukoman R. and Mitić-Ćulafić, Dragana",
year = "2015",
abstract = "AimTo evaluate in vitro cytotoxicity and in vivo inflammatory response to new nanostructural materials based on active calcium silicate systems (CS) and hydroxyapatite (HA-CS). MethodologyCytotoxicity of eluates of new nanostructural noncommercial materials CS and HA-CS, and MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil) as a control, were tested using the MTT assay on MRC-5 cells. Eluates of set materials were tested in 100% and 50% concentrations, 24h, 7days and 21days post-elution. The pH values were determined for undiluted eluates of set materials. Polyethylene tubes containing the test materials (CS, HA-CS, MTA) were implanted in subcutaneous tissue of Wistar rats. Histopathological examinations were conducted at 7, 15, 30 and 60days after the implantation. Data were statistically analyzed using three-way and one-way anova Tukeys post hoc test as well as Kruskall-Wallis test with Dunns post hoc test at =0.05. ResultsAll materials significantly reduced cell viability; especially when undiluted eluates were used (P LT 0.001). After 24h elution, cell viability was 101.8%, 49.5 +/- 4.2% and 61 +/- 7.4%, for MTA, and HA-CS, respectively. However, CS and HA-CS were significantly less toxic than the control material MTA (P LT 0.05). Cytotoxicity could be at least partially attributed to pH kinetics over time. Dilution of eluates of all tested materials resulted in better cell survival. Histopathological examination indicated similar inflammatory reaction, vascular congestion and connective tissue integrity associated with CS, HA-CS and MTA at each observation period (P GT 0.05). The only significant difference was found for capsule thickness, that is thicker capsule was associated with HA-CS compared to MTA at 60days (P=0.0039). HA-CS induced moderately thick capsules (median score 3, score range 2-3), whereas MTA resulted in thin capsule formation (median score 2, score range 1-3). ConclusionsEvaluation of cytotoxicity and inflammatory response indicated better biocompatibility of CS and HA-CS, in comparison with MTA (White MTA, Angelus((R)) Solucoes Odontologicas, Londrina, Brazil).",
journal = "International Endodontic Journal",
title = "Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study",
volume = "48",
number = "10",
pages = "966-975",
doi = "10.1111/iej.12391"
}

Petrović, V., Opacic-Galic, V., Zivkovic, S., Nikolić, B., Danilovic, V., Miletic, V., Jokanović, V. R.,& Mitić-Ćulafić, D.. (2015). Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study. in International Endodontic Journal, 48(10), 966-975.
https://doi.org/10.1111/iej.12391

Petrović V, Opacic-Galic V, Zivkovic S, Nikolić B, Danilovic V, Miletic V, Jokanović VR, Mitić-Ćulafić D. Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study. in International Endodontic Journal. 2015;48(10):966-975.
doi:10.1111/iej.12391 .

Petrović, V., Opacic-Galic, V., Zivkovic, S., Nikolić, Biljana, Danilovic, V., Miletic, V., Jokanović, Vukoman R., Mitić-Ćulafić, Dragana, "Biocompatibility of new nanostructural materials based on active silicate systems and hydroxyapatite: in vitro and in vivo study" in International Endodontic Journal, 48, no. 10 (2015):966-975,
https://doi.org/10.1111/iej.12391 . .

TY  - JOUR
AU  - Opacic-Galic, V.
AU  - Petrovic, V.
AU  - Zivkovic, S.
AU  - Jokanović, Vukoman R.
AU  - Nikolić, Biljana
AU  - Knežević-Vukčević, Jelena
AU  - Mitić-Ćulafić, Dragana
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5501
AB  - Aim To characterize and investigate the genotoxic effect of a new endodontic cement based on dicalcium- and tricalcium-silicate (CS) with hydroxyapatite (HA) on human lymphocytes. Methodology Hydrothermal treatment was applied for synthesis of CS and HA. The final mixture HA-CS, with potential to be used in endodontic practice, is composed of CS (34%) and HA (66%). Human lymphocytes were incubated with HA, HA-CS and CS for 1h, at 37 degrees C and 5% CO2. Cell viability was determined using the trypan blue exclusion assay. To evaluate the level of DNA damage comet assay (single cell gel electrophoresis) was performed. For the statistical analysis anova and Duncans Post Hoc Test were used. Results The SEM analysis indicated that CS consisted mostly of agglomerates of several micrometers in size, built up from smaller particles, with dimensions between 117 and 477nm. This is promising because dimensions of agglomerates are not comparable with channels inside the cell membranes, whereas their nano-elements provide evident activity, important for faster setting of these mixtures compared to MTA. Values of DNA damage obtained in the comet assay indicated low genotoxic risk of the new endodontic materials. Conclusions The significantly improved setting characteristics and low genotoxic risk of the new material support further research.
T2  - International Endodontic Journal
T1  - New nanostructural biomaterials based on active silicate systems and hydroxyapatite: characterization and genotoxicity in human peripheral blood lymphocytes
VL  - 46
IS  - 6
SP  - 506
EP  - 516
DO  - 10.1111/iej.12017
ER  - 

@article{
author = "Opacic-Galic, V. and Petrovic, V. and Zivkovic, S. and Jokanović, Vukoman R. and Nikolić, Biljana and Knežević-Vukčević, Jelena and Mitić-Ćulafić, Dragana",
year = "2013",
abstract = "Aim To characterize and investigate the genotoxic effect of a new endodontic cement based on dicalcium- and tricalcium-silicate (CS) with hydroxyapatite (HA) on human lymphocytes. Methodology Hydrothermal treatment was applied for synthesis of CS and HA. The final mixture HA-CS, with potential to be used in endodontic practice, is composed of CS (34%) and HA (66%). Human lymphocytes were incubated with HA, HA-CS and CS for 1h, at 37 degrees C and 5% CO2. Cell viability was determined using the trypan blue exclusion assay. To evaluate the level of DNA damage comet assay (single cell gel electrophoresis) was performed. For the statistical analysis anova and Duncans Post Hoc Test were used. Results The SEM analysis indicated that CS consisted mostly of agglomerates of several micrometers in size, built up from smaller particles, with dimensions between 117 and 477nm. This is promising because dimensions of agglomerates are not comparable with channels inside the cell membranes, whereas their nano-elements provide evident activity, important for faster setting of these mixtures compared to MTA. Values of DNA damage obtained in the comet assay indicated low genotoxic risk of the new endodontic materials. Conclusions The significantly improved setting characteristics and low genotoxic risk of the new material support further research.",
journal = "International Endodontic Journal",
title = "New nanostructural biomaterials based on active silicate systems and hydroxyapatite: characterization and genotoxicity in human peripheral blood lymphocytes",
volume = "46",
number = "6",
pages = "506-516",
doi = "10.1111/iej.12017"
}

Opacic-Galic, V., Petrovic, V., Zivkovic, S., Jokanović, V. R., Nikolić, B., Knežević-Vukčević, J.,& Mitić-Ćulafić, D.. (2013). New nanostructural biomaterials based on active silicate systems and hydroxyapatite: characterization and genotoxicity in human peripheral blood lymphocytes. in International Endodontic Journal, 46(6), 506-516.
https://doi.org/10.1111/iej.12017

Opacic-Galic V, Petrovic V, Zivkovic S, Jokanović VR, Nikolić B, Knežević-Vukčević J, Mitić-Ćulafić D. New nanostructural biomaterials based on active silicate systems and hydroxyapatite: characterization and genotoxicity in human peripheral blood lymphocytes. in International Endodontic Journal. 2013;46(6):506-516.
doi:10.1111/iej.12017 .

Opacic-Galic, V., Petrovic, V., Zivkovic, S., Jokanović, Vukoman R., Nikolić, Biljana, Knežević-Vukčević, Jelena, Mitić-Ćulafić, Dragana, "New nanostructural biomaterials based on active silicate systems and hydroxyapatite: characterization and genotoxicity in human peripheral blood lymphocytes" in International Endodontic Journal, 46, no. 6 (2013):506-516,
https://doi.org/10.1111/iej.12017 . .