TY  - JOUR
AU  - Spremo-Potparević, Biljana
AU  - Bajić, Vladan P.
AU  - Perry, George
AU  - Živković, Lada
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/844
AB  - Chromosomal alterations as a sign of genetic instability are a feature of Alzheimers disease (AD). Assessment of the genetic instability of non-neuronal cells of AD patients may provide a method to diagnose or monitor prognosis of the disease. Considering the importance of X chromosome alterations in the possible etiology of AD females, we used fluorescent in situ hybridization (FISH) for the centromere region of the X chromosome to determine aneuploidy, for a possible correlation with premature centromere division (PCD, X) in lymphocytes of AD females and age-matched controls. In AD patients, our results showed a marked and significant increase in the frequency of the X chromosome aneuploidy comparing with age matched controls (p LT 0.001). Also, a significant difference was detected in the PCD, X frequency between AD females when compared with age matched controls (p LT 0.001). In addition, a strong (R2=0.97, n=20) and significant (p LT 0.001) correlation was found between the frequency of aneuploidy and PCD, X in the AD group. Our results support the view that AD is a generalized systematic disease where PCD is to be considered as a stable sign of disease leading to aneuploidy.
PB  - Bentham Science Publishers
T2  - Current Alzheimer Research
T1  - Alterations of the X Chromosome in Lymphocytes of Alzheimers Disease Patients
VL  - 12
IS  - 10
SP  - 990
EP  - 996
DO  - 10.2174/1567205012666151027124154
ER  - 

@article{
author = "Spremo-Potparević, Biljana and Bajić, Vladan P. and Perry, George and Živković, Lada",
year = "2015",
abstract = "Chromosomal alterations as a sign of genetic instability are a feature of Alzheimers disease (AD). Assessment of the genetic instability of non-neuronal cells of AD patients may provide a method to diagnose or monitor prognosis of the disease. Considering the importance of X chromosome alterations in the possible etiology of AD females, we used fluorescent in situ hybridization (FISH) for the centromere region of the X chromosome to determine aneuploidy, for a possible correlation with premature centromere division (PCD, X) in lymphocytes of AD females and age-matched controls. In AD patients, our results showed a marked and significant increase in the frequency of the X chromosome aneuploidy comparing with age matched controls (p LT 0.001). Also, a significant difference was detected in the PCD, X frequency between AD females when compared with age matched controls (p LT 0.001). In addition, a strong (R2=0.97, n=20) and significant (p LT 0.001) correlation was found between the frequency of aneuploidy and PCD, X in the AD group. Our results support the view that AD is a generalized systematic disease where PCD is to be considered as a stable sign of disease leading to aneuploidy.",
publisher = "Bentham Science Publishers",
journal = "Current Alzheimer Research",
title = "Alterations of the X Chromosome in Lymphocytes of Alzheimers Disease Patients",
volume = "12",
number = "10",
pages = "990-996",
doi = "10.2174/1567205012666151027124154"
}

Spremo-Potparević, B., Bajić, V. P., Perry, G.,& Živković, L.. (2015). Alterations of the X Chromosome in Lymphocytes of Alzheimers Disease Patients. in Current Alzheimer Research
Bentham Science Publishers., 12(10), 990-996.
https://doi.org/10.2174/1567205012666151027124154

Spremo-Potparević B, Bajić VP, Perry G, Živković L. Alterations of the X Chromosome in Lymphocytes of Alzheimers Disease Patients. in Current Alzheimer Research. 2015;12(10):990-996.
doi:10.2174/1567205012666151027124154 .

Spremo-Potparević, Biljana, Bajić, Vladan P., Perry, George, Živković, Lada, "Alterations of the X Chromosome in Lymphocytes of Alzheimers Disease Patients" in Current Alzheimer Research, 12, no. 10 (2015):990-996,
https://doi.org/10.2174/1567205012666151027124154 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
AU  - Siedlak, Sandra L.
AU  - Perry, George
AU  - Plećaš-Solarović, Bosiljka
AU  - Milicević, Zorana
AU  - Bajić, Vladan P.
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5681
AB  - While Alzheimer disease (AD) is considered a neurodegenerative disorder, the importance of chromosome instability in non-neuronal cells is equally important, not only for shedding light on the etiology of the disease, but also for possible diagnostic purposes and monitoring the progress of the disease. Here, we evaluated the frequency of DNA damage and expression of premature centromere division (PCD) in peripheral blood lymphocytes of sporadic AD patients, age-matched and young controls. The results show that in male patients with AD, the frequencies of PCD and DNA damage were significantly greater (88%, p LT 0.01 and 38%, p LT 0.05, respectively) than in age-matched control group. AD females had significantly increased frequency of PCD (134%, p LT 0.01) as well as a higher frequency of DNA damage (37%, p LT 0.05). Ageing per se, both in males and females, shows significant increase of percentages of PCD (2.3 times, p LT 0.01 and 2.8 times, p LT 0.01, respectively) and DNA damage (63%, p LT 0.01 and 50%, p LT 0.01, respectively) comparing with young controls. In addition, a strong (R-2 = 0.873, n = 6) and significant (p LT 0.01) correlation between the frequencies of PCD and DNA damage was found in all examined groups. We may conclude that the increases in both parameters evaluated in this study are not only associated with normal ageing processes, but are markedly and significantly intensified in AD pathogenesis. Thus, our data support the view that AD is a generalized systemic disease, at least as for the increased DNA damage and PCD incidence in peripheral blood cells. copyright (C) 2013 S. Karger AG, Basel
T2  - Neurodegenerative diseases
T1  - DNA Damage in Alzheimer Disease Lymphocytes and Its Relation to Premature Centromere Division
VL  - 12
IS  - 3
SP  - 156
EP  - 163
DO  - 10.1159/000346114
ER  - 

@article{
author = "Živković, Lada and Spremo-Potparević, Biljana and Siedlak, Sandra L. and Perry, George and Plećaš-Solarović, Bosiljka and Milicević, Zorana and Bajić, Vladan P.",
year = "2013",
abstract = "While Alzheimer disease (AD) is considered a neurodegenerative disorder, the importance of chromosome instability in non-neuronal cells is equally important, not only for shedding light on the etiology of the disease, but also for possible diagnostic purposes and monitoring the progress of the disease. Here, we evaluated the frequency of DNA damage and expression of premature centromere division (PCD) in peripheral blood lymphocytes of sporadic AD patients, age-matched and young controls. The results show that in male patients with AD, the frequencies of PCD and DNA damage were significantly greater (88%, p LT 0.01 and 38%, p LT 0.05, respectively) than in age-matched control group. AD females had significantly increased frequency of PCD (134%, p LT 0.01) as well as a higher frequency of DNA damage (37%, p LT 0.05). Ageing per se, both in males and females, shows significant increase of percentages of PCD (2.3 times, p LT 0.01 and 2.8 times, p LT 0.01, respectively) and DNA damage (63%, p LT 0.01 and 50%, p LT 0.01, respectively) comparing with young controls. In addition, a strong (R-2 = 0.873, n = 6) and significant (p LT 0.01) correlation between the frequencies of PCD and DNA damage was found in all examined groups. We may conclude that the increases in both parameters evaluated in this study are not only associated with normal ageing processes, but are markedly and significantly intensified in AD pathogenesis. Thus, our data support the view that AD is a generalized systemic disease, at least as for the increased DNA damage and PCD incidence in peripheral blood cells. copyright (C) 2013 S. Karger AG, Basel",
journal = "Neurodegenerative diseases",
title = "DNA Damage in Alzheimer Disease Lymphocytes and Its Relation to Premature Centromere Division",
volume = "12",
number = "3",
pages = "156-163",
doi = "10.1159/000346114"
}

Živković, L., Spremo-Potparević, B., Siedlak, S. L., Perry, G., Plećaš-Solarović, B., Milicević, Z.,& Bajić, V. P.. (2013). DNA Damage in Alzheimer Disease Lymphocytes and Its Relation to Premature Centromere Division. in Neurodegenerative diseases, 12(3), 156-163.
https://doi.org/10.1159/000346114

Živković L, Spremo-Potparević B, Siedlak SL, Perry G, Plećaš-Solarović B, Milicević Z, Bajić VP. DNA Damage in Alzheimer Disease Lymphocytes and Its Relation to Premature Centromere Division. in Neurodegenerative diseases. 2013;12(3):156-163.
doi:10.1159/000346114 .

Živković, Lada, Spremo-Potparević, Biljana, Siedlak, Sandra L., Perry, George, Plećaš-Solarović, Bosiljka, Milicević, Zorana, Bajić, Vladan P., "DNA Damage in Alzheimer Disease Lymphocytes and Its Relation to Premature Centromere Division" in Neurodegenerative diseases, 12, no. 3 (2013):156-163,
https://doi.org/10.1159/000346114 . .