TY  - CONF
AU  - Stanković, Aleksandra
AU  - Jovanović, Ivan G.
AU  - Dinčić, E.
AU  - Vojinović, S.
AU  - Stojković, Ljiljana S.
AU  - Đorđević, Ana
AU  - Kuveljić, Jovana
AU  - Živković, Maja
PY  - 2023
UR  - https://web.archive.org/web/20240131090652/https://cony2023.comtecmed.com/e-posters/
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12641
AB  - Molecular background and biomarkers of highly heterogenous and hardly predictable disease progression among relapse-onset MS patients are of high research interest. In the current pilot study, we aimed to employ next-generation sequencing to investigate the expression of whole small non-coding microRNAs (miRNome) in two groups of MS patients with highly distinctive progression phenotype: one with fast progressing, severely disabling course vs. mild course of MS, longitudinally followed 10 years. Peripheral blood mononuclear cells (PBMC) miRNome data was obtained from mild phenotype MS (n=4 patients) and progressive phenotype MS (n=5 patients), using TakaraBio SMARTer smRNA-Seq Kit on iSeq100 (Illumina). Pre-processing of raw sequencing data, quality control and miRNA differential expression analysis was performed using sRNAtoolbox pipeline. Functional interpretation of differentially expressed miRNA target genes was done in DIANA-miRPathv3.0. Tarbase v8.0 served as a resource of miRNA:gene interactions. Achieved read depth was approximately 1 million raw reads/sample, allowing detection of up to 92 mature miRNAs after genome alignment and miRbase v22 annotation. Differential expression analysis identified the significant upregulation of hsa-miR-23c (log2FC=4.29, Padj= 0.03) in progressive phenotype. Top significantly enriched KEGG pathways in hsa-miR-23c targets suggested regulation of molecular pathways involved in autoimmunity (antigen presentation, Epstein-Barr virus infection) and cancer. In conclusion, this pilot study indicates phenotype-related differences in expression of miRNAs, molecules with high regulatory and biomarker properties. Although detected in PBMC, has-miR-23c is highly expressed in the brain and target MS relevant genes such as, HLA (A, B, C), transferrin receptor, Nrf2, recently proposed to play important role in neurodegeneration.
C3  - 17th World Congress on Controversies in Neurology (CONy) : e-posters
T1  - Insight in miRNome of severe multiple sclerosis: Pilot study of distinctive relapse-onset MS phenotypes
SP  - 427
EP  - 427
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12641
ER  - 

@conference{
author = "Stanković, Aleksandra and Jovanović, Ivan G. and Dinčić, E. and Vojinović, S. and Stojković, Ljiljana S. and Đorđević, Ana and Kuveljić, Jovana and Živković, Maja",
year = "2023",
abstract = "Molecular background and biomarkers of highly heterogenous and hardly predictable disease progression among relapse-onset MS patients are of high research interest. In the current pilot study, we aimed to employ next-generation sequencing to investigate the expression of whole small non-coding microRNAs (miRNome) in two groups of MS patients with highly distinctive progression phenotype: one with fast progressing, severely disabling course vs. mild course of MS, longitudinally followed 10 years. Peripheral blood mononuclear cells (PBMC) miRNome data was obtained from mild phenotype MS (n=4 patients) and progressive phenotype MS (n=5 patients), using TakaraBio SMARTer smRNA-Seq Kit on iSeq100 (Illumina). Pre-processing of raw sequencing data, quality control and miRNA differential expression analysis was performed using sRNAtoolbox pipeline. Functional interpretation of differentially expressed miRNA target genes was done in DIANA-miRPathv3.0. Tarbase v8.0 served as a resource of miRNA:gene interactions. Achieved read depth was approximately 1 million raw reads/sample, allowing detection of up to 92 mature miRNAs after genome alignment and miRbase v22 annotation. Differential expression analysis identified the significant upregulation of hsa-miR-23c (log2FC=4.29, Padj= 0.03) in progressive phenotype. Top significantly enriched KEGG pathways in hsa-miR-23c targets suggested regulation of molecular pathways involved in autoimmunity (antigen presentation, Epstein-Barr virus infection) and cancer. In conclusion, this pilot study indicates phenotype-related differences in expression of miRNAs, molecules with high regulatory and biomarker properties. Although detected in PBMC, has-miR-23c is highly expressed in the brain and target MS relevant genes such as, HLA (A, B, C), transferrin receptor, Nrf2, recently proposed to play important role in neurodegeneration.",
journal = "17th World Congress on Controversies in Neurology (CONy) : e-posters",
title = "Insight in miRNome of severe multiple sclerosis: Pilot study of distinctive relapse-onset MS phenotypes",
pages = "427-427",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12641"
}

Stanković, A., Jovanović, I. G., Dinčić, E., Vojinović, S., Stojković, L. S., Đorđević, A., Kuveljić, J.,& Živković, M.. (2023). Insight in miRNome of severe multiple sclerosis: Pilot study of distinctive relapse-onset MS phenotypes. in 17th World Congress on Controversies in Neurology (CONy) : e-posters, 427-427.
https://hdl.handle.net/21.15107/rcub_vinar_12641

Stanković A, Jovanović IG, Dinčić E, Vojinović S, Stojković LS, Đorđević A, Kuveljić J, Živković M. Insight in miRNome of severe multiple sclerosis: Pilot study of distinctive relapse-onset MS phenotypes. in 17th World Congress on Controversies in Neurology (CONy) : e-posters. 2023;:427-427.
https://hdl.handle.net/21.15107/rcub_vinar_12641 .

Stanković, Aleksandra, Jovanović, Ivan G., Dinčić, E., Vojinović, S., Stojković, Ljiljana S., Đorđević, Ana, Kuveljić, Jovana, Živković, Maja, "Insight in miRNome of severe multiple sclerosis: Pilot study of distinctive relapse-onset MS phenotypes" in 17th World Congress on Controversies in Neurology (CONy) : e-posters (2023):427-427,
https://hdl.handle.net/21.15107/rcub_vinar_12641 .

TY  - CONF
AU  - Stojković, Ljiljana
AU  - Stanković, Aleksandra
AU  - Dinčić, Evica
AU  - Živković, Maja
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2738
C3  - Multiple Sclerosis Journal
T1  - The association of CXCL16 A181V and I142T gene polymorphisms with multiple sclerosis
VL  - 17
SP  - S339
EP  - S339
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2738
ER  - 

@conference{
author = "Stojković, Ljiljana and Stanković, Aleksandra and Dinčić, Evica and Živković, Maja",
year = "2011",
journal = "Multiple Sclerosis Journal",
title = "The association of CXCL16 A181V and I142T gene polymorphisms with multiple sclerosis",
volume = "17",
pages = "S339-S339",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2738"
}

Stojković, L., Stanković, A., Dinčić, E.,& Živković, M.. (2011). The association of CXCL16 A181V and I142T gene polymorphisms with multiple sclerosis. in Multiple Sclerosis Journal, 17, S339-S339.
https://hdl.handle.net/21.15107/rcub_vinar_2738

Stojković L, Stanković A, Dinčić E, Živković M. The association of CXCL16 A181V and I142T gene polymorphisms with multiple sclerosis. in Multiple Sclerosis Journal. 2011;17:S339-S339.
https://hdl.handle.net/21.15107/rcub_vinar_2738 .

Stojković, Ljiljana, Stanković, Aleksandra, Dinčić, Evica, Živković, Maja, "The association of CXCL16 A181V and I142T gene polymorphisms with multiple sclerosis" in Multiple Sclerosis Journal, 17 (2011):S339-S339,
https://hdl.handle.net/21.15107/rcub_vinar_2738 .