@conference{
author = "Milovanović, Jelena and Hranisavljević, Jelena and Vujasinović, Tijana and Todorović-Raković, Nataša",
year = "2023",
abstract = "B ackground: Interleukin-8 (IL-8) is a mulƟ funcƟ onal cytokine linked to cancer progression. Studies have confi rmed high IL-8 levels in HER2-enriched and basal-like (ER–) primary breast tumors. The aim of this study was to evaluate the relaƟ onship between intratumoral IL-8 protein levels and clinical outcome in hormone dependent (ER+) primary breast cancer paƟ ents. PaƟ ents and methods: The study included 65 early-stage breast cancer paƟ ents with detectable levels of hormone receptors (ER>0, PR>0), all of whom had not received any prior hormonal or chemotherapeuƟ c systemic therapy. The median follow-up was 144 months. Steroid hormone receptor status was determined by ligand-binding assay. HER2 status (absence or presence of gene amplifi caƟ on) was determined by chromogenic in situ hybridizaƟ on (CISH). IL-8 protein levels were determined in cytosol tumor extracts by quanƟ taƟ ve ELISA. ER level of 10 fmol/mg, PR level of 20 fmol/mg and the median IL-8 concentraƟ on level of 88.8 pg/mg, were used as cut-off values. Results: There was a signifi cant diff erence in relapse free survival (RFS) between IL8low and IL8high subgroups of paƟ ents (Log rank test, p=0.002). Considering subgroups of paƟ ents straƟ fi ed in diff erent phenotypes according to receptor status and the median IL-8 value, if IL-8 is highly expressed, the infl uence of ER is weaker and there was no signifi cant diff erence in RFS between subgroups with ERlowIL8high and ERhighIL8high phenotypes. The same is true for PR and HER2 and there was no signifi cant diff erence in RFS between subgroups with PRlowIL8high and PRhighIL8high phenotypes, neither between subgroups with HER2−IL8high and HER2+IL8high phenotypes. On the other hand, subgroup with ERhighIL8low phenotype had signifi cantly longer RFS compared to those with ERlowIL8high and ERhighIL8high phenotypes (p=0.02, p=0.04, respecƟ vely); subgroup with PRlowIL8low phenotype had signifi cantly longer RFS compared to those with PRlowIL8high and PRhighIL8high phenotypes (p=0.003, p=0.02, respecƟ vely); and subgroup with HER2–IL8low phenotype had signifi cantly longer RFS compared to those with HER2–IL8high and HER2+IL8high phenotypes (p=0.01, p=0.02, respecƟ vely). Conclusions: IL-8 is a potenƟ al biomarker of unfavorable prognosis in hormone dependent breast cancer that is associated with the established parameters ER/PR and HER2. Receptor-mediated signaling could act addiƟ vely with IL-8 signaling in progression of hormone dependent breast cancer",
publisher = "Belgrade : Serbian Association for Cancer Research",
journal = "Oncology Insights",
title = "The significance of interleukin-8 in hormonally dependent early breast cancer – association with the established parameters ER/PR and HER2",
number = "1",
pages = "84-85",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12635"
}
