TY  - JOUR
AU  - Stefanović, Milan
AU  - Stojković, Ljiljana
AU  - Životić, Ivan
AU  - Dinčić, Evica
AU  - Stanković, Aleksandra
AU  - Živković, Maja
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12715
AB  - Multiple sclerosis (MS), a noncurable autoimmune neurodegenerative disease, requires constant research that could improve understanding of both environmental and genetic factors that lead to its occurrence and/or progression. Recognition of the genetic basis of MS further leads to an investigation of the regulatory role of genetic variants on gene expression. Among risk variants for MS, Ikaros zinc finger 3 (IKZF3) gene variant rs12946510 was identified as one of the top-ranked and the expression quantitative trait loci (eQTL) for genes residing in chromosomal locus 17q12- 21. The study aimed to investigate the association of gene expression of the immunologically relevant genes, which map to indicated locus, ORMDL3, GSDMB, and IKZF3, with MS and rs12946510 genotype, taking into account disease phase, clinical parameters of disease progression, and severity and immunomodulatory therapy. We used TaqMan® technology for both allelic discrimination and gene expression determination in 67 relapsing MS patients and 50 healthy controls. Decreased ORMDL3 and GSDMB mRNA levels had significant associations with MS and rs12946510 TT rare homozygote among patients. Significant positive correlations between ORMDL3 and GSDMB mRNA expression were observed in both patients and controls. We detected the significant between-effect of sex and rs12946510 on the expression of ORMDL3 in the patient group and interferon β therapy and rs12946510 on GSDMB expression. Our results show the association of ORMDL3 and GSDMB mRNA expression with the clinical manifestation of MS and confirm that IKZF3 rs12946510 exerts the eQTL effect on both genes in multiple sclerosis. Besides providing novel insight related to MS phases and interferon β therapy, the study results confirm previous studies on regulatory genetic variants, autoimmunity, and MS.
T2  - Heliyon
T1  - Expression levels of GSDMB and ORMDL3 are associated with relapsing-remitting multiple sclerosis and IKZF3 rs12946510 variant
VL  - 10
IS  - 3
SP  - e25033
DO  - 10.1016/j.heliyon.2024.e25033
ER  - 

@article{
author = "Stefanović, Milan and Stojković, Ljiljana and Životić, Ivan and Dinčić, Evica and Stanković, Aleksandra and Živković, Maja",
year = "2024",
abstract = "Multiple sclerosis (MS), a noncurable autoimmune neurodegenerative disease, requires constant research that could improve understanding of both environmental and genetic factors that lead to its occurrence and/or progression. Recognition of the genetic basis of MS further leads to an investigation of the regulatory role of genetic variants on gene expression. Among risk variants for MS, Ikaros zinc finger 3 (IKZF3) gene variant rs12946510 was identified as one of the top-ranked and the expression quantitative trait loci (eQTL) for genes residing in chromosomal locus 17q12- 21. The study aimed to investigate the association of gene expression of the immunologically relevant genes, which map to indicated locus, ORMDL3, GSDMB, and IKZF3, with MS and rs12946510 genotype, taking into account disease phase, clinical parameters of disease progression, and severity and immunomodulatory therapy. We used TaqMan® technology for both allelic discrimination and gene expression determination in 67 relapsing MS patients and 50 healthy controls. Decreased ORMDL3 and GSDMB mRNA levels had significant associations with MS and rs12946510 TT rare homozygote among patients. Significant positive correlations between ORMDL3 and GSDMB mRNA expression were observed in both patients and controls. We detected the significant between-effect of sex and rs12946510 on the expression of ORMDL3 in the patient group and interferon β therapy and rs12946510 on GSDMB expression. Our results show the association of ORMDL3 and GSDMB mRNA expression with the clinical manifestation of MS and confirm that IKZF3 rs12946510 exerts the eQTL effect on both genes in multiple sclerosis. Besides providing novel insight related to MS phases and interferon β therapy, the study results confirm previous studies on regulatory genetic variants, autoimmunity, and MS.",
journal = "Heliyon",
title = "Expression levels of GSDMB and ORMDL3 are associated with relapsing-remitting multiple sclerosis and IKZF3 rs12946510 variant",
volume = "10",
number = "3",
pages = "e25033",
doi = "10.1016/j.heliyon.2024.e25033"
}

Stefanović, M., Stojković, L., Životić, I., Dinčić, E., Stanković, A.,& Živković, M.. (2024). Expression levels of GSDMB and ORMDL3 are associated with relapsing-remitting multiple sclerosis and IKZF3 rs12946510 variant. in Heliyon, 10(3), e25033.
https://doi.org/10.1016/j.heliyon.2024.e25033

Stefanović M, Stojković L, Životić I, Dinčić E, Stanković A, Živković M. Expression levels of GSDMB and ORMDL3 are associated with relapsing-remitting multiple sclerosis and IKZF3 rs12946510 variant. in Heliyon. 2024;10(3):e25033.
doi:10.1016/j.heliyon.2024.e25033 .

Stefanović, Milan, Stojković, Ljiljana, Životić, Ivan, Dinčić, Evica, Stanković, Aleksandra, Živković, Maja, "Expression levels of GSDMB and ORMDL3 are associated with relapsing-remitting multiple sclerosis and IKZF3 rs12946510 variant" in Heliyon, 10, no. 3 (2024):e25033,
https://doi.org/10.1016/j.heliyon.2024.e25033 . .

TY  - JOUR
AU  - Mitrović, Kristina
AU  - Životić, Ivan
AU  - Kolić, Ivana
AU  - Žakula, Jelena
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Jovanović, Ivan
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12889
AB  - Background The majority of CAKUT-associated CNVs overlap at least one miRNA gene, thus affecting the cellular levels of the corresponding miRNA. We aimed to investigate the potency of restitution of CNV-affected miRNA levels to remediate the dysregulated expression of target genes involved in kidney physiology and development in vitro.  Methods Heterozygous MIR484 knockout HEK293 and homozygous MIR185 knockout HEK293 cell lines were used as models depicting the deletion of the frequently affected miRNA genes by CAKUT-associated CNVs. After treatment with the corresponding miRNA mimics, the levels of the target genes have been compared to the non-targeting control treatment. For both investigated miRNAs, MDM2 and PKD1 were evaluated as common targets, while additional 3 genes were investigated as targets of each individual miRNA (NOTCH3, FIS1 and APAF1 as hsa-miR-484 targets and RHOA, ATF6 and CDC42 as hsa-miR-185-5p targets).  Results Restitution of the corresponding miRNA levels in both knockout cell lines has induced a change in the mRNA levels of certain candidate target genes, thus confirming the potential to alleviate the CNV effect on miRNA expression. Intriguingly, HEK293 WT treatment with investigated miRNA mimics has triggered a more pronounced effect, thus suggesting the importance of miRNA interplay in different genomic contexts.  Conclusions Dysregulation of multiple mRNA targets mediated by CNV-affected miRNAs could represent the underlying mechanism behind the unresolved CAKUT occurrence and phenotypic variability observed in CAKUT patients. Characterizing miRNAs located in CNVs and their potential to become molecular targets could eventually help in understanding and improving the management of CAKUT.
T2  - BMC Genomics
T1  - A preliminary study of the miRNA restitution effect on CNV-induced miRNA downregulation in CAKUT
VL  - 25
IS  - 1
SP  - 218
DO  - 10.1186/s12864-024-10121-8
ER  - 

@article{
author = "Mitrović, Kristina and Životić, Ivan and Kolić, Ivana and Žakula, Jelena and Živković, Maja and Stanković, Aleksandra and Jovanović, Ivan",
year = "2024",
abstract = "Background The majority of CAKUT-associated CNVs overlap at least one miRNA gene, thus affecting the cellular levels of the corresponding miRNA. We aimed to investigate the potency of restitution of CNV-affected miRNA levels to remediate the dysregulated expression of target genes involved in kidney physiology and development in vitro.  Methods Heterozygous MIR484 knockout HEK293 and homozygous MIR185 knockout HEK293 cell lines were used as models depicting the deletion of the frequently affected miRNA genes by CAKUT-associated CNVs. After treatment with the corresponding miRNA mimics, the levels of the target genes have been compared to the non-targeting control treatment. For both investigated miRNAs, MDM2 and PKD1 were evaluated as common targets, while additional 3 genes were investigated as targets of each individual miRNA (NOTCH3, FIS1 and APAF1 as hsa-miR-484 targets and RHOA, ATF6 and CDC42 as hsa-miR-185-5p targets).  Results Restitution of the corresponding miRNA levels in both knockout cell lines has induced a change in the mRNA levels of certain candidate target genes, thus confirming the potential to alleviate the CNV effect on miRNA expression. Intriguingly, HEK293 WT treatment with investigated miRNA mimics has triggered a more pronounced effect, thus suggesting the importance of miRNA interplay in different genomic contexts.  Conclusions Dysregulation of multiple mRNA targets mediated by CNV-affected miRNAs could represent the underlying mechanism behind the unresolved CAKUT occurrence and phenotypic variability observed in CAKUT patients. Characterizing miRNAs located in CNVs and their potential to become molecular targets could eventually help in understanding and improving the management of CAKUT.",
journal = "BMC Genomics",
title = "A preliminary study of the miRNA restitution effect on CNV-induced miRNA downregulation in CAKUT",
volume = "25",
number = "1",
pages = "218",
doi = "10.1186/s12864-024-10121-8"
}

Mitrović, K., Životić, I., Kolić, I., Žakula, J., Živković, M., Stanković, A.,& Jovanović, I.. (2024). A preliminary study of the miRNA restitution effect on CNV-induced miRNA downregulation in CAKUT. in BMC Genomics, 25(1), 218.
https://doi.org/10.1186/s12864-024-10121-8

Mitrović K, Životić I, Kolić I, Žakula J, Živković M, Stanković A, Jovanović I. A preliminary study of the miRNA restitution effect on CNV-induced miRNA downregulation in CAKUT. in BMC Genomics. 2024;25(1):218.
doi:10.1186/s12864-024-10121-8 .

Mitrović, Kristina, Životić, Ivan, Kolić, Ivana, Žakula, Jelena, Živković, Maja, Stanković, Aleksandra, Jovanović, Ivan, "A preliminary study of the miRNA restitution effect on CNV-induced miRNA downregulation in CAKUT" in BMC Genomics, 25, no. 1 (2024):218,
https://doi.org/10.1186/s12864-024-10121-8 . .

TY  - CONF
AU  - Životić, Ivan
AU  - Mitrović, Kristina
AU  - Kolić, Ivana
AU  - Seke, Mariana
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Jovanović, Ivan
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12465
AB  - Introduction: Congenital anomalies of the kidney and urinary tracts(CAKUT) are a diverse spectrum of defects with complex etiology and not fully explained genetic background. miRNA-containing copy number variants (CNVs) are described as genetic risk factor for the disease development. We aimed to identify miRNAs with the maximum regulatory coverage of previously reported differentially expressed genes in CAKUT tissue compared to controls and bioinformatically characterize a set of these miRNAs which are located in common CNVs. Methods: Differentially expressed genes were identified from ureter tissue transcriptome open data GSE83946 from 15 CAKUT patients and 7 healthy controls, generated in house previously. miRPathDB v2.0 was used for identification of miRNAs with maximum coverage of DEGs(miRNAs which complimentarily regulate all DEGs). Mapping of maximum coverage miRNAs onto common CNVs (frequency >0.2) was performed using UCSC genome browser and gnomAD database. miRNA mapping common CNVs were further bioinformatically analyzed using miRPathDB v2.0. Results: In a maximum coverage set of 50 miRNAs interacting with DEGs in CAKUT, we have identified 3 miRNA geneslocated in the common CNVs(hsa-miR-663b, hsa-miR-3180-3p and hsa-miR-1302). Using Reactome database we identified all three miRNAsto be significantly enriched in the pathway Neuronal System: -log(p-value)>2.326 for hsa-miR-1302; -log(p-value)>1.556 for hsa-miR-3180-3p; and -log(pvalue)>1.703 for hsa-miR-663b. Conclusion: CAKUT is characterized with variable penetrability and expressivity and often followed with other comorbiditiessuch as neurodevelopmental disorders. miRNAsinvolved in DEG networks and prone to CNV effects could present modulating factors of the disease phenotype. Further studies should provide additional evidence about hsa-miR-1302, hsa-miR-3180-3p and hsa-miR-663b involvements in CAKUT etiology
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts
T1  - Identification of micro RNA from common copy number variants as risk factors for CAKUT
SP  - 62
EP  - 62
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12465
ER  - 

@conference{
author = "Životić, Ivan and Mitrović, Kristina and Kolić, Ivana and Seke, Mariana and Živković, Maja and Stanković, Aleksandra and Jovanović, Ivan",
year = "2023",
abstract = "Introduction: Congenital anomalies of the kidney and urinary tracts(CAKUT) are a diverse spectrum of defects with complex etiology and not fully explained genetic background. miRNA-containing copy number variants (CNVs) are described as genetic risk factor for the disease development. We aimed to identify miRNAs with the maximum regulatory coverage of previously reported differentially expressed genes in CAKUT tissue compared to controls and bioinformatically characterize a set of these miRNAs which are located in common CNVs. Methods: Differentially expressed genes were identified from ureter tissue transcriptome open data GSE83946 from 15 CAKUT patients and 7 healthy controls, generated in house previously. miRPathDB v2.0 was used for identification of miRNAs with maximum coverage of DEGs(miRNAs which complimentarily regulate all DEGs). Mapping of maximum coverage miRNAs onto common CNVs (frequency >0.2) was performed using UCSC genome browser and gnomAD database. miRNA mapping common CNVs were further bioinformatically analyzed using miRPathDB v2.0. Results: In a maximum coverage set of 50 miRNAs interacting with DEGs in CAKUT, we have identified 3 miRNA geneslocated in the common CNVs(hsa-miR-663b, hsa-miR-3180-3p and hsa-miR-1302). Using Reactome database we identified all three miRNAsto be significantly enriched in the pathway Neuronal System: -log(p-value)>2.326 for hsa-miR-1302; -log(p-value)>1.556 for hsa-miR-3180-3p; and -log(pvalue)>1.703 for hsa-miR-663b. Conclusion: CAKUT is characterized with variable penetrability and expressivity and often followed with other comorbiditiessuch as neurodevelopmental disorders. miRNAsinvolved in DEG networks and prone to CNV effects could present modulating factors of the disease phenotype. Further studies should provide additional evidence about hsa-miR-1302, hsa-miR-3180-3p and hsa-miR-663b involvements in CAKUT etiology",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts",
title = "Identification of micro RNA from common copy number variants as risk factors for CAKUT",
pages = "62-62",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12465"
}

Životić, I., Mitrović, K., Kolić, I., Seke, M., Živković, M., Stanković, A.,& Jovanović, I.. (2023). Identification of micro RNA from common copy number variants as risk factors for CAKUT. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts, 62-62.
https://hdl.handle.net/21.15107/rcub_vinar_12465

Životić I, Mitrović K, Kolić I, Seke M, Živković M, Stanković A, Jovanović I. Identification of micro RNA from common copy number variants as risk factors for CAKUT. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts. 2023;:62-62.
https://hdl.handle.net/21.15107/rcub_vinar_12465 .

Životić, Ivan, Mitrović, Kristina, Kolić, Ivana, Seke, Mariana, Živković, Maja, Stanković, Aleksandra, Jovanović, Ivan, "Identification of micro RNA from common copy number variants as risk factors for CAKUT" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts (2023):62-62,
https://hdl.handle.net/21.15107/rcub_vinar_12465 .

TY  - CONF
AU  - Kuveljić, Jovana
AU  - Životić, Ivan
AU  - Dekleva, Milica
AU  - Živković, Maja
AU  - Đurić, Tamara
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12648
AB  - Introduction: Myocardial infarction (MI) and consequential ischemia with cardiomyocyte loss are followed by left ventricular (LV) remodeling. LV remodeling is crucial process for cardiac function preservation, although when prolonged it can become maladaptive and lead to impaired systolic function and further cardiovascular complications. Echocardiographic parameters are used as a measure of LV structure and function. ADAM17 (a disintegrin and metalloprotease domain) and CDKN1A (cyclindependent kinase inhibitor 1A) have shown regulating role in DNA repair, inflammation, remodeling and fibrosis. The aim of this preliminary study was to investigate the potential effect of CDKN1 and ADAM17 mRNA in post MI heart remodeling. Methods: Sixty four patients with the first MI were prospectively followed-up 6 months after MI. Change (Δ) of echocardiographic parameters within 6 months was calculated as a difference between the value at 6-month follow-up and value at admission. Relative gene expression was detected using the TaqMan® technology. Statistical analyses were done by Statistica 8 software. Results: We have observed correlation between CDKN1A mRNA expression and change of LV enddiastolic diameter (ΔLVEDD, R=0.3, p=0.01) and LV end-systolic diameter (ΔLVESD, R=0.3, p=0.02), but not with LV ejection fraction and stroke volume. ADAM17 expression was not in correlation with analyzed parameters of LV remodeling. However, CDKN1A and ADAM17 mRNA expression in PBMC six months after MI were positively correlated (R=0.6, p<0.001). Conclusion: Preliminary resultssuggest that CDKN1 has a role in post MI LV remodeling, correlating with changes in echocardiographic parameters of LV structure. The validation on a larger sample size is required.
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts
T1  - Correlations of CDKN1A and ADAM17 expression with a change of left ventricular remodeling echocardiographic parameters in PBMC of patients six months after the first myocardial infarction
SP  - 53
EP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12648
ER  - 

@conference{
author = "Kuveljić, Jovana and Životić, Ivan and Dekleva, Milica and Živković, Maja and Đurić, Tamara",
year = "2023",
abstract = "Introduction: Myocardial infarction (MI) and consequential ischemia with cardiomyocyte loss are followed by left ventricular (LV) remodeling. LV remodeling is crucial process for cardiac function preservation, although when prolonged it can become maladaptive and lead to impaired systolic function and further cardiovascular complications. Echocardiographic parameters are used as a measure of LV structure and function. ADAM17 (a disintegrin and metalloprotease domain) and CDKN1A (cyclindependent kinase inhibitor 1A) have shown regulating role in DNA repair, inflammation, remodeling and fibrosis. The aim of this preliminary study was to investigate the potential effect of CDKN1 and ADAM17 mRNA in post MI heart remodeling. Methods: Sixty four patients with the first MI were prospectively followed-up 6 months after MI. Change (Δ) of echocardiographic parameters within 6 months was calculated as a difference between the value at 6-month follow-up and value at admission. Relative gene expression was detected using the TaqMan® technology. Statistical analyses were done by Statistica 8 software. Results: We have observed correlation between CDKN1A mRNA expression and change of LV enddiastolic diameter (ΔLVEDD, R=0.3, p=0.01) and LV end-systolic diameter (ΔLVESD, R=0.3, p=0.02), but not with LV ejection fraction and stroke volume. ADAM17 expression was not in correlation with analyzed parameters of LV remodeling. However, CDKN1A and ADAM17 mRNA expression in PBMC six months after MI were positively correlated (R=0.6, p<0.001). Conclusion: Preliminary resultssuggest that CDKN1 has a role in post MI LV remodeling, correlating with changes in echocardiographic parameters of LV structure. The validation on a larger sample size is required.",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts",
title = "Correlations of CDKN1A and ADAM17 expression with a change of left ventricular remodeling echocardiographic parameters in PBMC of patients six months after the first myocardial infarction",
pages = "53-53",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12648"
}

Kuveljić, J., Životić, I., Dekleva, M., Živković, M.,& Đurić, T.. (2023). Correlations of CDKN1A and ADAM17 expression with a change of left ventricular remodeling echocardiographic parameters in PBMC of patients six months after the first myocardial infarction. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts, 53-53.
https://hdl.handle.net/21.15107/rcub_vinar_12648

Kuveljić J, Životić I, Dekleva M, Živković M, Đurić T. Correlations of CDKN1A and ADAM17 expression with a change of left ventricular remodeling echocardiographic parameters in PBMC of patients six months after the first myocardial infarction. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts. 2023;:53-53.
https://hdl.handle.net/21.15107/rcub_vinar_12648 .

Kuveljić, Jovana, Životić, Ivan, Dekleva, Milica, Živković, Maja, Đurić, Tamara, "Correlations of CDKN1A and ADAM17 expression with a change of left ventricular remodeling echocardiographic parameters in PBMC of patients six months after the first myocardial infarction" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia : Book of abstracts (2023):53-53,
https://hdl.handle.net/21.15107/rcub_vinar_12648 .

TY  - JOUR
AU  - Mitrović, Kristina
AU  - Životić, Ivan
AU  - Kolić, Ivana
AU  - Đorđević, Ana
AU  - Žakula, Jelena
AU  - Filipović Tričković, Jelena G.
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Jovanović, Ivan G.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10469
AB  - Rare copy number variants (CNVs) are among the most common genomic disorders underlying CAKUT. miRNAs located in rare CNVs represent well-founded functional variants for human CAKUT research. The study aimed to identify and functionally interpret miRNAs most frequently affected by rare CNVs in CAKUT and to estimate the overall burden of rare CNVs on miRNA genes in CAKUT. The additional aim of this study was to experimentally confirm the effect of a rare CNV in CAKUT on candidate miRNA’s expression and the subsequent change in mRNA levels of selected target genes. A database of CAKUT-associated rare CNV regions, created by literature mining, was used for mapping of the miRNA precursors. miRNAs and miRNA families, most frequently affected by rare CAKUT-associated CNVs, have been subjected to bioinformatic analysis. CNV burden analysis was performed to identify chromosomes with over/underrepresentation of miRNA genes in rare CNVs associated with CAKUT. A functional study was performed on HEK293 MIR484+/- KO and HEK293 WT cell lines, followed by the analysis of relative miRNA and mRNA target gene levels. 80% of CAKUT patients with underlying rare CNV had at least one miRNA gene overlapping the identified CNV. Network analysis of the most frequently affected miRNAs has revealed the dominant regulation of the two miRNAs, hsa-miR-484 and hsa-miR-185-5p. Additionally, miR-548 family members have shown substantial enrichment in rare CNVs in CAKUT. An over/underrepresentation of miRNA genes in rare CNVs associated with CAKUT was observed in multiple chromosomes, such as chr16, chr20, and chr21. A significant 0.37 fold downregulation of hsa-miR-484, followed by a notable upregulation of MDM2 and APAF1 and downregulation of NOTCH3 was detected in HEK293 MIR484+/- KO compared to HEK293 WT cell lines, supporting the study hypothesis. miRNA genes are frequently affected by rare CNVs in CAKUT patients. Understanding the potential of CNV-affected miRNAs to participate in CAKUT as genetic drivers represent a crucial implication for the development of novel therapeutic approaches.
T2  - Scientific Reports
T1  - Identification and functional interpretation of miRNAs affected by rare CNVs in CAKUT
VL  - 12
IS  - 1
SP  - 17746
DO  - 10.1038/s41598-022-22749-1
ER  - 

@article{
author = "Mitrović, Kristina and Životić, Ivan and Kolić, Ivana and Đorđević, Ana and Žakula, Jelena and Filipović Tričković, Jelena G. and Živković, Maja and Stanković, Aleksandra and Jovanović, Ivan G.",
year = "2022",
abstract = "Rare copy number variants (CNVs) are among the most common genomic disorders underlying CAKUT. miRNAs located in rare CNVs represent well-founded functional variants for human CAKUT research. The study aimed to identify and functionally interpret miRNAs most frequently affected by rare CNVs in CAKUT and to estimate the overall burden of rare CNVs on miRNA genes in CAKUT. The additional aim of this study was to experimentally confirm the effect of a rare CNV in CAKUT on candidate miRNA’s expression and the subsequent change in mRNA levels of selected target genes. A database of CAKUT-associated rare CNV regions, created by literature mining, was used for mapping of the miRNA precursors. miRNAs and miRNA families, most frequently affected by rare CAKUT-associated CNVs, have been subjected to bioinformatic analysis. CNV burden analysis was performed to identify chromosomes with over/underrepresentation of miRNA genes in rare CNVs associated with CAKUT. A functional study was performed on HEK293 MIR484+/- KO and HEK293 WT cell lines, followed by the analysis of relative miRNA and mRNA target gene levels. 80% of CAKUT patients with underlying rare CNV had at least one miRNA gene overlapping the identified CNV. Network analysis of the most frequently affected miRNAs has revealed the dominant regulation of the two miRNAs, hsa-miR-484 and hsa-miR-185-5p. Additionally, miR-548 family members have shown substantial enrichment in rare CNVs in CAKUT. An over/underrepresentation of miRNA genes in rare CNVs associated with CAKUT was observed in multiple chromosomes, such as chr16, chr20, and chr21. A significant 0.37 fold downregulation of hsa-miR-484, followed by a notable upregulation of MDM2 and APAF1 and downregulation of NOTCH3 was detected in HEK293 MIR484+/- KO compared to HEK293 WT cell lines, supporting the study hypothesis. miRNA genes are frequently affected by rare CNVs in CAKUT patients. Understanding the potential of CNV-affected miRNAs to participate in CAKUT as genetic drivers represent a crucial implication for the development of novel therapeutic approaches.",
journal = "Scientific Reports",
title = "Identification and functional interpretation of miRNAs affected by rare CNVs in CAKUT",
volume = "12",
number = "1",
pages = "17746",
doi = "10.1038/s41598-022-22749-1"
}

Mitrović, K., Životić, I., Kolić, I., Đorđević, A., Žakula, J., Filipović Tričković, J. G., Živković, M., Stanković, A.,& Jovanović, I. G.. (2022). Identification and functional interpretation of miRNAs affected by rare CNVs in CAKUT. in Scientific Reports, 12(1), 17746.
https://doi.org/10.1038/s41598-022-22749-1

Mitrović K, Životić I, Kolić I, Đorđević A, Žakula J, Filipović Tričković JG, Živković M, Stanković A, Jovanović IG. Identification and functional interpretation of miRNAs affected by rare CNVs in CAKUT. in Scientific Reports. 2022;12(1):17746.
doi:10.1038/s41598-022-22749-1 .

Mitrović, Kristina, Životić, Ivan, Kolić, Ivana, Đorđević, Ana, Žakula, Jelena, Filipović Tričković, Jelena G., Živković, Maja, Stanković, Aleksandra, Jovanović, Ivan G., "Identification and functional interpretation of miRNAs affected by rare CNVs in CAKUT" in Scientific Reports, 12, no. 1 (2022):17746,
https://doi.org/10.1038/s41598-022-22749-1 . .

TY  - CONF
AU  - Mitrović, Kristina
AU  - Kolić, Ivana
AU  - Životić, Ivan
AU  - Filipović Tričković, Jelena G.
AU  - Đorđević, Ana
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Jovanović, Ivan G.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10830
AB  - Introduction: miR-548 family members, located on all human chromosomes except chr19 and chrY, regulate podocyte differentiation in vitro, important for kidney development. Rare copy number variants (rCNVs) are the common genetic cause of Congenital Anomalies of the Kidney and Urinary Tract (CAKUT) and could harbour miRNAs. The aim of this study was to investigate to which extent rCNVs associated with CAKUT harbour miR-548 members.
Materials and Methods: Extensive literature review was conducted to collect data of pathogenic and likely pathogenic rCNVs in CAKUT patients. UCSC genome browser tool was employed for mapping of miR-548 members onto collected rCNV regions and gnomAD SV controls database. Bioinformatic analysis was conducted using miRPathDB2 tool.
Results: We generated CAKUT database of pathogenic CNVs in 79 chromosome regions from 191 patient and likely pathogenic CNVs in 74 regions from 87 patients. Pathogenic rCNVs of seventeen patients, located on 7 chromosomes, contained at least one miR-548 member. Likely pathogenic rCNVs of 4 patients, located on 3 chromosomes, contained one of miR-548 members. Bioinformatic analysis implied the role of mapped miRNAs in the regulation of processes associated with CAKUT. In controls, only hsa-mir-548i-3 (out of 73 precursors) was mapped on polymorphic CNVs (af>1%) and wasn’t identified in patients.
Conclusions: miR-548 members located in rCNVs should be investigated in future studies as potential genetic drivers of CAKUT development, beyond protein coding genes.
C3  - European Journal of Human Genetics
T1  - Are miR-548 family members potential genetic drivers of CAKUT
VL  - 30
IS  - Suppl. 1
SP  - 331
DO  - 10.1038/s41431-021-01026-1
ER  - 

@conference{
author = "Mitrović, Kristina and Kolić, Ivana and Životić, Ivan and Filipović Tričković, Jelena G. and Đorđević, Ana and Živković, Maja and Stanković, Aleksandra and Jovanović, Ivan G.",
year = "2022",
abstract = "Introduction: miR-548 family members, located on all human chromosomes except chr19 and chrY, regulate podocyte differentiation in vitro, important for kidney development. Rare copy number variants (rCNVs) are the common genetic cause of Congenital Anomalies of the Kidney and Urinary Tract (CAKUT) and could harbour miRNAs. The aim of this study was to investigate to which extent rCNVs associated with CAKUT harbour miR-548 members.
Materials and Methods: Extensive literature review was conducted to collect data of pathogenic and likely pathogenic rCNVs in CAKUT patients. UCSC genome browser tool was employed for mapping of miR-548 members onto collected rCNV regions and gnomAD SV controls database. Bioinformatic analysis was conducted using miRPathDB2 tool.
Results: We generated CAKUT database of pathogenic CNVs in 79 chromosome regions from 191 patient and likely pathogenic CNVs in 74 regions from 87 patients. Pathogenic rCNVs of seventeen patients, located on 7 chromosomes, contained at least one miR-548 member. Likely pathogenic rCNVs of 4 patients, located on 3 chromosomes, contained one of miR-548 members. Bioinformatic analysis implied the role of mapped miRNAs in the regulation of processes associated with CAKUT. In controls, only hsa-mir-548i-3 (out of 73 precursors) was mapped on polymorphic CNVs (af>1%) and wasn’t identified in patients.
Conclusions: miR-548 members located in rCNVs should be investigated in future studies as potential genetic drivers of CAKUT development, beyond protein coding genes.",
journal = "European Journal of Human Genetics",
title = "Are miR-548 family members potential genetic drivers of CAKUT",
volume = "30",
number = "Suppl. 1",
pages = "331",
doi = "10.1038/s41431-021-01026-1"
}

Mitrović, K., Kolić, I., Životić, I., Filipović Tričković, J. G., Đorđević, A., Živković, M., Stanković, A.,& Jovanović, I. G.. (2022). Are miR-548 family members potential genetic drivers of CAKUT. in European Journal of Human Genetics, 30(Suppl. 1), 331.
https://doi.org/10.1038/s41431-021-01026-1

Mitrović K, Kolić I, Životić I, Filipović Tričković JG, Đorđević A, Živković M, Stanković A, Jovanović IG. Are miR-548 family members potential genetic drivers of CAKUT. in European Journal of Human Genetics. 2022;30(Suppl. 1):331.
doi:10.1038/s41431-021-01026-1 .

Mitrović, Kristina, Kolić, Ivana, Životić, Ivan, Filipović Tričković, Jelena G., Đorđević, Ana, Živković, Maja, Stanković, Aleksandra, Jovanović, Ivan G., "Are miR-548 family members potential genetic drivers of CAKUT" in European Journal of Human Genetics, 30, no. Suppl. 1 (2022):331,
https://doi.org/10.1038/s41431-021-01026-1 . .

TY  - CONF
AU  - Životić, Ivan
AU  - Kolić, Ivana
AU  - Popić, Kristina
AU  - Filipović Tričković, Jelena G.
AU  - Đorđević, Ana
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Jovanović, Ivan G.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10831
AB  - Introduction: Genetic studies of congenital anomalies of the kidney and urinary tract (CAKUT) have demonstrated variable penetrability and expressivity of the associated genetic defects. Previously, it was shown that deletions of 17q12 and 22q11.2 regions were specific for kidney anomalies (KA) while 16p11.2 and 1q21.1 loci showed extensive pleiotropy in CAKUT phenotypes. CNVs affecting miRNA gene dosage have been described to have functional influence on gene expression. We aimed to conduct comprehensive in silico analysis using publicly available databases to analyze miRNA content of CAKUT-associated CNVs in quoted chromosomal loci with regard to pleiotropy.  Methods: Extensive literature review was conducted to collect data about pathogenic rCNVs associated with CAKUT. UCSC genome browser tool was employed for mapping miRNAs onto collected rCNV regions.  Results: Analysis of CNVs in CAKUT included four studies counting more than 2500 patients. In further analysis we included 191 patients harboring pathogenic CNVs. Surprisingly, CAKUT pleiotropic regions (16p11.2, 1q21.2) did not contain any miRNA. 22q11.2 showed the densest miRNAs content (n = 21).  Conclusions: Absence of miRNAs may potentially pronounce the pleiotropy of the CAKUT genetic defects, thus leading to the variety of phenotypes. Contrary, abundancy of miRNAs in 22q11.2 might be associated with reproducible phenotype, such as KA, producing the functional effect when deleted. This assumption agrees with recent results of miRNA expression variability in 22q11.2 deletion syndrome.
C3  - European Journal of Human Genetics
T1  - miRNA-free rare pathogenic CNVs could drive toward variable CAKUT phenotypes
VL  - 30
IS  - Suppl. 1
SP  - 331
DO  - 10.1038/s41431-021-01026-1
ER  - 

@conference{
author = "Životić, Ivan and Kolić, Ivana and Popić, Kristina and Filipović Tričković, Jelena G. and Đorđević, Ana and Živković, Maja and Stanković, Aleksandra and Jovanović, Ivan G.",
year = "2022",
abstract = "Introduction: Genetic studies of congenital anomalies of the kidney and urinary tract (CAKUT) have demonstrated variable penetrability and expressivity of the associated genetic defects. Previously, it was shown that deletions of 17q12 and 22q11.2 regions were specific for kidney anomalies (KA) while 16p11.2 and 1q21.1 loci showed extensive pleiotropy in CAKUT phenotypes. CNVs affecting miRNA gene dosage have been described to have functional influence on gene expression. We aimed to conduct comprehensive in silico analysis using publicly available databases to analyze miRNA content of CAKUT-associated CNVs in quoted chromosomal loci with regard to pleiotropy.  Methods: Extensive literature review was conducted to collect data about pathogenic rCNVs associated with CAKUT. UCSC genome browser tool was employed for mapping miRNAs onto collected rCNV regions.  Results: Analysis of CNVs in CAKUT included four studies counting more than 2500 patients. In further analysis we included 191 patients harboring pathogenic CNVs. Surprisingly, CAKUT pleiotropic regions (16p11.2, 1q21.2) did not contain any miRNA. 22q11.2 showed the densest miRNAs content (n = 21).  Conclusions: Absence of miRNAs may potentially pronounce the pleiotropy of the CAKUT genetic defects, thus leading to the variety of phenotypes. Contrary, abundancy of miRNAs in 22q11.2 might be associated with reproducible phenotype, such as KA, producing the functional effect when deleted. This assumption agrees with recent results of miRNA expression variability in 22q11.2 deletion syndrome.",
journal = "European Journal of Human Genetics",
title = "miRNA-free rare pathogenic CNVs could drive toward variable CAKUT phenotypes",
volume = "30",
number = "Suppl. 1",
pages = "331",
doi = "10.1038/s41431-021-01026-1"
}

Životić, I., Kolić, I., Popić, K., Filipović Tričković, J. G., Đorđević, A., Živković, M., Stanković, A.,& Jovanović, I. G.. (2022). miRNA-free rare pathogenic CNVs could drive toward variable CAKUT phenotypes. in European Journal of Human Genetics, 30(Suppl. 1), 331.
https://doi.org/10.1038/s41431-021-01026-1

Životić I, Kolić I, Popić K, Filipović Tričković JG, Đorđević A, Živković M, Stanković A, Jovanović IG. miRNA-free rare pathogenic CNVs could drive toward variable CAKUT phenotypes. in European Journal of Human Genetics. 2022;30(Suppl. 1):331.
doi:10.1038/s41431-021-01026-1 .

Životić, Ivan, Kolić, Ivana, Popić, Kristina, Filipović Tričković, Jelena G., Đorđević, Ana, Živković, Maja, Stanković, Aleksandra, Jovanović, Ivan G., "miRNA-free rare pathogenic CNVs could drive toward variable CAKUT phenotypes" in European Journal of Human Genetics, 30, no. Suppl. 1 (2022):331,
https://doi.org/10.1038/s41431-021-01026-1 . .

TY  - CONF
AU  - Kolić, Ivana
AU  - Mitrović, Kristina
AU  - Životić, Ivan
AU  - Đorđević, Ana
AU  - Filipović-Tričković, Jelena
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Jovanović, Ivan
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12684
AB  - Background/Objectives: Rare copy number variants (rCNVs) are the common genetic cause of Congenital Anomalies of the Kidney and Urinary Tract (CAKUT).1 miRNAs located in rCNVs represent well-founded functional variants for human CAKUT research. However, the impact of rCNVs on miRNA genes in CAKUT is unknown. Thus, burden assessment was performed to identify chromosomes with non-random representation of miRNA genes in rCNVs associated with CAKUT. Methods: A comprehensive literature mining of rCNV regions associated with CAKUT was performed. The total cumulative length of rCNVs per chromosome was the sum of corresponding CNV-DNA regions, taking into account overlapping. Mapping of miRNAs onto cumulative rCNV regions gave counts of affected miRNA loci. The correlation analysis was performed between the number of miRNA genes overlapping rCNVs, and the fractional lengths of cumulative rCNVs regions in relation to the chromosome size. Results: A statistically significant positive correlation was observed for duplications and deletions respectively (Spearman correlation p<0.0001, r=0.9, r=0.8). However, a deviation from the best fit line for chromosome 16, for both rare duplications and deletions, was observed due to the high overrepresentation of miRNA genes in identified rCNVs. Conclusion: The current finding of the high overall burden of rCNVs on miRNA genes in chromosome 16 suggests that miRNAs located on this chromosome could serve as candidates for the investigation of miRNA role in CAKUT development.
C3  - 54th European Society of Human Genetics (ESHG) : Book of abstracts
T1  - Assessing the burden of rare CNVs on miRNA genes in CAKUT
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12684
ER  - 

@conference{
author = "Kolić, Ivana and Mitrović, Kristina and Životić, Ivan and Đorđević, Ana and Filipović-Tričković, Jelena and Živković, Maja and Stanković, Aleksandra and Jovanović, Ivan",
year = "2022",
abstract = "Background/Objectives: Rare copy number variants (rCNVs) are the common genetic cause of Congenital Anomalies of the Kidney and Urinary Tract (CAKUT).1 miRNAs located in rCNVs represent well-founded functional variants for human CAKUT research. However, the impact of rCNVs on miRNA genes in CAKUT is unknown. Thus, burden assessment was performed to identify chromosomes with non-random representation of miRNA genes in rCNVs associated with CAKUT. Methods: A comprehensive literature mining of rCNV regions associated with CAKUT was performed. The total cumulative length of rCNVs per chromosome was the sum of corresponding CNV-DNA regions, taking into account overlapping. Mapping of miRNAs onto cumulative rCNV regions gave counts of affected miRNA loci. The correlation analysis was performed between the number of miRNA genes overlapping rCNVs, and the fractional lengths of cumulative rCNVs regions in relation to the chromosome size. Results: A statistically significant positive correlation was observed for duplications and deletions respectively (Spearman correlation p<0.0001, r=0.9, r=0.8). However, a deviation from the best fit line for chromosome 16, for both rare duplications and deletions, was observed due to the high overrepresentation of miRNA genes in identified rCNVs. Conclusion: The current finding of the high overall burden of rCNVs on miRNA genes in chromosome 16 suggests that miRNAs located on this chromosome could serve as candidates for the investigation of miRNA role in CAKUT development.",
journal = "54th European Society of Human Genetics (ESHG) : Book of abstracts",
title = "Assessing the burden of rare CNVs on miRNA genes in CAKUT",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12684"
}

Kolić, I., Mitrović, K., Životić, I., Đorđević, A., Filipović-Tričković, J., Živković, M., Stanković, A.,& Jovanović, I.. (2022). Assessing the burden of rare CNVs on miRNA genes in CAKUT. in 54th European Society of Human Genetics (ESHG) : Book of abstracts.
https://hdl.handle.net/21.15107/rcub_vinar_12684

Kolić I, Mitrović K, Životić I, Đorđević A, Filipović-Tričković J, Živković M, Stanković A, Jovanović I. Assessing the burden of rare CNVs on miRNA genes in CAKUT. in 54th European Society of Human Genetics (ESHG) : Book of abstracts. 2022;.
https://hdl.handle.net/21.15107/rcub_vinar_12684 .

Kolić, Ivana, Mitrović, Kristina, Životić, Ivan, Đorđević, Ana, Filipović-Tričković, Jelena, Živković, Maja, Stanković, Aleksandra, Jovanović, Ivan, "Assessing the burden of rare CNVs on miRNA genes in CAKUT" in 54th European Society of Human Genetics (ESHG) : Book of abstracts (2022),
https://hdl.handle.net/21.15107/rcub_vinar_12684 .

TY  - JOUR
AU  - Stojković, Ljiljana S.
AU  - Jovanović, Ivan G.
AU  - Živković, Maja
AU  - Zec, Manja
AU  - Đurić, Tamara
AU  - Životić, Ivan
AU  - Kuveljić, Jovana
AU  - Kolaković, Ana
AU  - Kolić, Ivana
AU  - Đorđević, Ana
AU  - Glibetić, Marija
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8683
AB  - Foods and food products that contain polyphenols are proposed to modulate risk of cardiovascular disease. The aim of this three-arm, crossover, randomized, double-blind, placebo-controlled intervention study was to examine the impact of Aronia melanocarpa juice (AMJ), high-polyphenol (AMJ treatment, 1.17 g/100 mL polyphenols) and low-polyphenol (dAMJ treatment, 0.29 g/100 mL polyphenols) dose, on the transcriptome in peripheral blood mononuclear cells (PBMC) of 19 subjects at cardiovascular risk. Transcriptome data were obtained by microarray. Bioinformatic functional annotation analysis was performed on both the whole transcriptome datasets and the differentially expressed genes (DEGs). Expression of selected DEGs was validated by RT-qPCR. Administration of AMJ and dAMJ treatments during the two consecutive four-week treatment periods had additive effects on PBMC transcriptome profiles, with the most pronounced and specific effect noticed for AMJ in the last treatment period (TP3) of the trial. Between the high-dose and low-dose treatments in TP3, there was a multitude of overlapping DEGs and DEG-enriched biological processes and pathways, which primarily included immunomodulation and regulation of cell proliferation/death. Increased expression of TNF, IL1B, IL8, RGS1, OSM, and DUSP2 in TP3 was confirmed by RT-qPCR. The results suggest the immunomodulatory effects of prolonged habitual consumption of polyphenol-rich aronia juice in individuals at cardiovascular risk.
T2  - Nutrients
T1  - The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk
VL  - 12
IS  - 5
SP  - 1484
DO  - 10.3390/nu12051484
ER  - 

@article{
author = "Stojković, Ljiljana S. and Jovanović, Ivan G. and Živković, Maja and Zec, Manja and Đurić, Tamara and Životić, Ivan and Kuveljić, Jovana and Kolaković, Ana and Kolić, Ivana and Đorđević, Ana and Glibetić, Marija and Alavantić, Dragan and Stanković, Aleksandra",
year = "2020",
abstract = "Foods and food products that contain polyphenols are proposed to modulate risk of cardiovascular disease. The aim of this three-arm, crossover, randomized, double-blind, placebo-controlled intervention study was to examine the impact of Aronia melanocarpa juice (AMJ), high-polyphenol (AMJ treatment, 1.17 g/100 mL polyphenols) and low-polyphenol (dAMJ treatment, 0.29 g/100 mL polyphenols) dose, on the transcriptome in peripheral blood mononuclear cells (PBMC) of 19 subjects at cardiovascular risk. Transcriptome data were obtained by microarray. Bioinformatic functional annotation analysis was performed on both the whole transcriptome datasets and the differentially expressed genes (DEGs). Expression of selected DEGs was validated by RT-qPCR. Administration of AMJ and dAMJ treatments during the two consecutive four-week treatment periods had additive effects on PBMC transcriptome profiles, with the most pronounced and specific effect noticed for AMJ in the last treatment period (TP3) of the trial. Between the high-dose and low-dose treatments in TP3, there was a multitude of overlapping DEGs and DEG-enriched biological processes and pathways, which primarily included immunomodulation and regulation of cell proliferation/death. Increased expression of TNF, IL1B, IL8, RGS1, OSM, and DUSP2 in TP3 was confirmed by RT-qPCR. The results suggest the immunomodulatory effects of prolonged habitual consumption of polyphenol-rich aronia juice in individuals at cardiovascular risk.",
journal = "Nutrients",
title = "The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk",
volume = "12",
number = "5",
pages = "1484",
doi = "10.3390/nu12051484"
}

Stojković, L. S., Jovanović, I. G., Živković, M., Zec, M., Đurić, T., Životić, I., Kuveljić, J., Kolaković, A., Kolić, I., Đorđević, A., Glibetić, M., Alavantić, D.,& Stanković, A.. (2020). The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk. in Nutrients, 12(5), 1484.
https://doi.org/10.3390/nu12051484

Stojković LS, Jovanović IG, Živković M, Zec M, Đurić T, Životić I, Kuveljić J, Kolaković A, Kolić I, Đorđević A, Glibetić M, Alavantić D, Stanković A. The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk. in Nutrients. 2020;12(5):1484.
doi:10.3390/nu12051484 .

Stojković, Ljiljana S., Jovanović, Ivan G., Živković, Maja, Zec, Manja, Đurić, Tamara, Životić, Ivan, Kuveljić, Jovana, Kolaković, Ana, Kolić, Ivana, Đorđević, Ana, Glibetić, Marija, Alavantić, Dragan, Stanković, Aleksandra, "The Effects of Aronia melanocarpa Juice Consumption on the mRNA Expression Profile in Peripheral Blood Mononuclear Cells in Subjects at Cardiovascular Risk" in Nutrients, 12, no. 5 (2020):1484,
https://doi.org/10.3390/nu12051484 . .

TY  - JOUR
AU  - Stojković, Ljiljana S.
AU  - Stanković, Aleksandra
AU  - Životić, Ivan
AU  - Dinčić, Evica
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9725
AB  - Background/Aim. In vitro and in vivo studies show that CX3CL1 and CXCL16 chemokines and their specific receptors, CX3CR1 and CXCR6, respectively, mediate mechanism of neuroinflammation during the pathogenesis of multiple sclerosis (MS). The aim of this study was to investigate relative messenger ribonucleic acid (mRNA) levels of CX3CL1, CXCL16, CX3CR1 and CXCR6 in peripheral blood mononuclear cells, as potential molecular markers of relapsing-remitting (RR) MS. Methods. The study included 43 unrelated RR MS patients, 20 of them with clinically active disease (relapse) and 23 with clinically stable disease (remission), and 28 unrelated healthy subjects as controls. Real-time polymerase chain reactions (PCR) were performed using TaqMan® gene expression assays. Relative expression (mRNA) level of each target gene in each sample of peripheral blood mononuclear cells was calculated as the mean normalized expression. Results. The levels of CX3CR1 mRNA were significantly higher in clinically active RR MS patients compared to controls [fold change = 1.38, p (Mann-Whitney U test) = 0.009], and significantly lower in clinically stable vs active RR MS patients [fold change = - 1.43, p (t-test) = 0.03]. Stable RR MS patients had significantly higher CXCL16 mRNA levels than controls [fold change = 1.33, p (Mann-Whitney U test) = 0.006]. A trend of increased CXCR6 gene expression was found in active RR MS patients compared to controls [fold change = 1.23, p (Mann-Whitney U test) = 0.08]. In either active or stable RR MS patients there were no significant correlations of the clinical parameters with expression levels of the target genes. Conclusion. The current results show that increased CX3CR1 mRNA levels in peripheral blood mononuclear cells could represent a proinflammatory molecular marker of clinically active RR MS.
T2  - Vojnosanitetski pregled
T1  - Gene expression of chemokines CX3CL1 and CXCL16 and their receptors, CX3CR1 and CXCR6, in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis - a pilot study
VL  - 77
IS  - 9
SP  - 967
EP  - 973
DO  - 10.2298/VSP180717035S
ER  - 

@article{
author = "Stojković, Ljiljana S. and Stanković, Aleksandra and Životić, Ivan and Dinčić, Evica and Alavantić, Dragan and Živković, Maja",
year = "2020",
abstract = "Background/Aim. In vitro and in vivo studies show that CX3CL1 and CXCL16 chemokines and their specific receptors, CX3CR1 and CXCR6, respectively, mediate mechanism of neuroinflammation during the pathogenesis of multiple sclerosis (MS). The aim of this study was to investigate relative messenger ribonucleic acid (mRNA) levels of CX3CL1, CXCL16, CX3CR1 and CXCR6 in peripheral blood mononuclear cells, as potential molecular markers of relapsing-remitting (RR) MS. Methods. The study included 43 unrelated RR MS patients, 20 of them with clinically active disease (relapse) and 23 with clinically stable disease (remission), and 28 unrelated healthy subjects as controls. Real-time polymerase chain reactions (PCR) were performed using TaqMan® gene expression assays. Relative expression (mRNA) level of each target gene in each sample of peripheral blood mononuclear cells was calculated as the mean normalized expression. Results. The levels of CX3CR1 mRNA were significantly higher in clinically active RR MS patients compared to controls [fold change = 1.38, p (Mann-Whitney U test) = 0.009], and significantly lower in clinically stable vs active RR MS patients [fold change = - 1.43, p (t-test) = 0.03]. Stable RR MS patients had significantly higher CXCL16 mRNA levels than controls [fold change = 1.33, p (Mann-Whitney U test) = 0.006]. A trend of increased CXCR6 gene expression was found in active RR MS patients compared to controls [fold change = 1.23, p (Mann-Whitney U test) = 0.08]. In either active or stable RR MS patients there were no significant correlations of the clinical parameters with expression levels of the target genes. Conclusion. The current results show that increased CX3CR1 mRNA levels in peripheral blood mononuclear cells could represent a proinflammatory molecular marker of clinically active RR MS.",
journal = "Vojnosanitetski pregled",
title = "Gene expression of chemokines CX3CL1 and CXCL16 and their receptors, CX3CR1 and CXCR6, in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis - a pilot study",
volume = "77",
number = "9",
pages = "967-973",
doi = "10.2298/VSP180717035S"
}

Stojković, L. S., Stanković, A., Životić, I., Dinčić, E., Alavantić, D.,& Živković, M.. (2020). Gene expression of chemokines CX3CL1 and CXCL16 and their receptors, CX3CR1 and CXCR6, in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis - a pilot study. in Vojnosanitetski pregled, 77(9), 967-973.
https://doi.org/10.2298/VSP180717035S

Stojković LS, Stanković A, Životić I, Dinčić E, Alavantić D, Živković M. Gene expression of chemokines CX3CL1 and CXCL16 and their receptors, CX3CR1 and CXCR6, in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis - a pilot study. in Vojnosanitetski pregled. 2020;77(9):967-973.
doi:10.2298/VSP180717035S .

Stojković, Ljiljana S., Stanković, Aleksandra, Životić, Ivan, Dinčić, Evica, Alavantić, Dragan, Živković, Maja, "Gene expression of chemokines CX3CL1 and CXCL16 and their receptors, CX3CR1 and CXCR6, in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis - a pilot study" in Vojnosanitetski pregled, 77, no. 9 (2020):967-973,
https://doi.org/10.2298/VSP180717035S . .

TY  - JOUR
AU  - Stefanović, Milan
AU  - Životić, Ivan
AU  - Stojković, Ljiljana S.
AU  - Dinčić, Evica
AU  - Stanković, Aleksandra
AU  - Živković, Maja
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8885
AB  - An algorithm Probabilistic Identification of Causal SNPs, identified 434 causal variants for multiple sclerosis (MS) including IL2RA rs2104286, IFI30 rs11554159 and IKZF3 rs12946510. Analysis of individual and combined effects of these variants in the Serbian population identified that Il2RA rs2104286 G allele carriers had a lower risk for developing MS (gender adjusted OR = 0.63, p = .003). With regard to the IFI30 rs11554159 recessive genetic model, among HLA-DRB1*15:01 positive patients, the AA homozygote had a significantly higher MSSS compared to the G allele carriers (p = .003). This study confirms role of IL2RA rs2104286 in MS and suggest the role of IFI30 rs11554159 in disease severity, which needs validation.
T2  - Journal of Neuroimmunology
T1  - The association of genetic variants IL2RA rs2104286, IFI30 rs11554159 and IKZF3 rs12946510 with multiple sclerosis onset and severity in patients from Serbia
VL  - 347
SP  - 577346
DO  - 10.1016/j.jneuroim.2020.577346
ER  - 

@article{
author = "Stefanović, Milan and Životić, Ivan and Stojković, Ljiljana S. and Dinčić, Evica and Stanković, Aleksandra and Živković, Maja",
year = "2020",
abstract = "An algorithm Probabilistic Identification of Causal SNPs, identified 434 causal variants for multiple sclerosis (MS) including IL2RA rs2104286, IFI30 rs11554159 and IKZF3 rs12946510. Analysis of individual and combined effects of these variants in the Serbian population identified that Il2RA rs2104286 G allele carriers had a lower risk for developing MS (gender adjusted OR = 0.63, p = .003). With regard to the IFI30 rs11554159 recessive genetic model, among HLA-DRB1*15:01 positive patients, the AA homozygote had a significantly higher MSSS compared to the G allele carriers (p = .003). This study confirms role of IL2RA rs2104286 in MS and suggest the role of IFI30 rs11554159 in disease severity, which needs validation.",
journal = "Journal of Neuroimmunology",
title = "The association of genetic variants IL2RA rs2104286, IFI30 rs11554159 and IKZF3 rs12946510 with multiple sclerosis onset and severity in patients from Serbia",
volume = "347",
pages = "577346",
doi = "10.1016/j.jneuroim.2020.577346"
}

Stefanović, M., Životić, I., Stojković, L. S., Dinčić, E., Stanković, A.,& Živković, M.. (2020). The association of genetic variants IL2RA rs2104286, IFI30 rs11554159 and IKZF3 rs12946510 with multiple sclerosis onset and severity in patients from Serbia. in Journal of Neuroimmunology, 347, 577346.
https://doi.org/10.1016/j.jneuroim.2020.577346

Stefanović M, Životić I, Stojković LS, Dinčić E, Stanković A, Živković M. The association of genetic variants IL2RA rs2104286, IFI30 rs11554159 and IKZF3 rs12946510 with multiple sclerosis onset and severity in patients from Serbia. in Journal of Neuroimmunology. 2020;347:577346.
doi:10.1016/j.jneuroim.2020.577346 .

Stefanović, Milan, Životić, Ivan, Stojković, Ljiljana S., Dinčić, Evica, Stanković, Aleksandra, Živković, Maja, "The association of genetic variants IL2RA rs2104286, IFI30 rs11554159 and IKZF3 rs12946510 with multiple sclerosis onset and severity in patients from Serbia" in Journal of Neuroimmunology, 347 (2020):577346,
https://doi.org/10.1016/j.jneuroim.2020.577346 . .

TY  - JOUR
AU  - Stojković, Ljiljana S.
AU  - Stanković, Aleksandra
AU  - Životić, Ivan
AU  - Dinčić, Evica
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8884
AB  - Background/Aim. In vitro and in vivo studies show that CX3CL1 and CXCL16 chemokines and their specific receptors, CX3CR1 and CXCR6, respectively, mediate mechanism of neuroinflammation during the pathogenesis of multiple sclerosis (MS). The aim of this study was to investigate relative messenger ribonucleic acid (mRNA) levels of CX3CL1, CXCL16, CX3CR1 and CXCR6 in peripheral blood mononuclear cells, as potential molecular markers of relapsing-remitting (RR) MS. Methods. The study included 43 unrelated RR MS patients, 20 of them with clinically active disease (relapse) and 23 with clinically stable disease (remission), and 28 unrelated healthy subjects as controls. Real-time polymerase chain reactions (PCR) were performed using TaqMan? gene expression assays. Relative expression (mRNA) level of each target gene in each sample of peripheral blood mononuclear cells was calculated as the mean normalized expression. Results. The levels of CX3CR1 mRNA were significantly higher in clinically active RR MS patients compared to controls [fold change = 1.38, p (Mann-Whitney U test) = 0.009], and significantly lower in clinically stable vs active RR MS patients [fold change = - 1.43, p (t-test) = 0.03]. Stable RR MS patients had significantly higher CXCL16 mRNA levels than controls [fold change = 1.33, p (Mann-Whitney U test) = 0.006]. A trend of increased CXCR6 gene expression was found in active RR MS patients compared to controls [fold change = 1.23, p (Mann-Whitney U test) = 0.08]. In either active or stable RR MS patients there were no significant correlations of the clinical parameters with expression levels of the target genes. Conclusion. The current results show that increased CX3CR1 mRNA levels in peripheral blood mononuclear cells could represent a proinflammatory molecular marker of clinically active RR MS.
T2  - Vojnosanitetski pregled
T1  - Gene expression of chemokines CX3CL1 and CXCL16 and their receptors, CX3CR1 and CXCR6, in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis - a pilot study
VL  - 77
IS  - 9
SP  - 967
EP  - 973
DO  - 10.2298/VSP180717035S
ER  - 

@article{
author = "Stojković, Ljiljana S. and Stanković, Aleksandra and Životić, Ivan and Dinčić, Evica and Alavantić, Dragan and Živković, Maja",
year = "2020",
abstract = "Background/Aim. In vitro and in vivo studies show that CX3CL1 and CXCL16 chemokines and their specific receptors, CX3CR1 and CXCR6, respectively, mediate mechanism of neuroinflammation during the pathogenesis of multiple sclerosis (MS). The aim of this study was to investigate relative messenger ribonucleic acid (mRNA) levels of CX3CL1, CXCL16, CX3CR1 and CXCR6 in peripheral blood mononuclear cells, as potential molecular markers of relapsing-remitting (RR) MS. Methods. The study included 43 unrelated RR MS patients, 20 of them with clinically active disease (relapse) and 23 with clinically stable disease (remission), and 28 unrelated healthy subjects as controls. Real-time polymerase chain reactions (PCR) were performed using TaqMan? gene expression assays. Relative expression (mRNA) level of each target gene in each sample of peripheral blood mononuclear cells was calculated as the mean normalized expression. Results. The levels of CX3CR1 mRNA were significantly higher in clinically active RR MS patients compared to controls [fold change = 1.38, p (Mann-Whitney U test) = 0.009], and significantly lower in clinically stable vs active RR MS patients [fold change = - 1.43, p (t-test) = 0.03]. Stable RR MS patients had significantly higher CXCL16 mRNA levels than controls [fold change = 1.33, p (Mann-Whitney U test) = 0.006]. A trend of increased CXCR6 gene expression was found in active RR MS patients compared to controls [fold change = 1.23, p (Mann-Whitney U test) = 0.08]. In either active or stable RR MS patients there were no significant correlations of the clinical parameters with expression levels of the target genes. Conclusion. The current results show that increased CX3CR1 mRNA levels in peripheral blood mononuclear cells could represent a proinflammatory molecular marker of clinically active RR MS.",
journal = "Vojnosanitetski pregled",
title = "Gene expression of chemokines CX3CL1 and CXCL16 and their receptors, CX3CR1 and CXCR6, in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis - a pilot study",
volume = "77",
number = "9",
pages = "967-973",
doi = "10.2298/VSP180717035S"
}

Stojković, L. S., Stanković, A., Životić, I., Dinčić, E., Alavantić, D.,& Živković, M.. (2020). Gene expression of chemokines CX3CL1 and CXCL16 and their receptors, CX3CR1 and CXCR6, in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis - a pilot study. in Vojnosanitetski pregled, 77(9), 967-973.
https://doi.org/10.2298/VSP180717035S

Stojković LS, Stanković A, Životić I, Dinčić E, Alavantić D, Živković M. Gene expression of chemokines CX3CL1 and CXCL16 and their receptors, CX3CR1 and CXCR6, in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis - a pilot study. in Vojnosanitetski pregled. 2020;77(9):967-973.
doi:10.2298/VSP180717035S .

Stojković, Ljiljana S., Stanković, Aleksandra, Životić, Ivan, Dinčić, Evica, Alavantić, Dragan, Živković, Maja, "Gene expression of chemokines CX3CL1 and CXCL16 and their receptors, CX3CR1 and CXCR6, in peripheral blood mononuclear cells of patients with relapsing-remitting multiple sclerosis - a pilot study" in Vojnosanitetski pregled, 77, no. 9 (2020):967-973,
https://doi.org/10.2298/VSP180717035S . .

TY  - CONF
AU  - Životić, Ivan
AU  - Kolić, Ivana
AU  - Đurić, Tamara
AU  - Živković, Maja
AU  - Milovanović, Branislav
AU  - Stanković, Aleksandra
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12682
AB  - Introduction: The autonomic nervous system (ANS), plays an essential role in the regulation of vascular wall contractility, heart rate and blood pressure (BP) variability. The symptoms of ANS dysfunction were strongly associated with vasovagal syncope (VVS). The renin-angiotensin system (RAS) is a powerful feedback system responsible for long-term control of cardiovascular hemodynamic values, heart rate and BP homeostasis. All components of the RAS have been detected in the brain as well as in the cardiovascular system, with central and peripheral actions of angiotensin II main effector molecule synthesized by angiotensin converting enzyme (ACE). Opposite influences of angiotensin II (AngII) receptor type 1 (AT1R) and angiotensin II receptor type 2 (ATR2) on sympathetic tone have been documented. Genetic studies have shown that individuals with VVS usually have a positive family history. Still, the genetic basis of VVS is unclear. The aim of the study was to investigate the association of the RAS gene variants, ACE I/D, AT1R A1166C and ATR2 -1332 A/G with the VVS in Serbian population. Methods: This case-control designed study included 215 VVS patients and 439 controls (C) matched by age (mean±SD, VVS: 38,25±13,52 years and C: 36,63±11,69 years). Genotyping was done by PCR-RFLP and allele specific PCR methods. Results: There were no significant differences in the genotype frequency distributions of ACE I/D and AT1R A1166C variants between cases and controls (p=0.4 and p=0.2, respectively). With regard to X-linked ATR2 -1332 A/G variant the frequency of A/- hemizygotes was significantly higher in VVS than in controls, in males (p=0.01). In females there were no differences in genotype frequency distributions between cases and controls (p=0.73). Conclusions: Our results suggest further investigation of AT2R hemodynamic effect on AngII in VVS males. To accurately elucidate given association, replication of the results in a larger sample is inevitable.
C3  - ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts
T1  - Renin-angiotensin system gene variants in association with vasovagal syncope in Serbian population
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12682
ER  - 

@conference{
author = "Životić, Ivan and Kolić, Ivana and Đurić, Tamara and Živković, Maja and Milovanović, Branislav and Stanković, Aleksandra",
year = "2019",
abstract = "Introduction: The autonomic nervous system (ANS), plays an essential role in the regulation of vascular wall contractility, heart rate and blood pressure (BP) variability. The symptoms of ANS dysfunction were strongly associated with vasovagal syncope (VVS). The renin-angiotensin system (RAS) is a powerful feedback system responsible for long-term control of cardiovascular hemodynamic values, heart rate and BP homeostasis. All components of the RAS have been detected in the brain as well as in the cardiovascular system, with central and peripheral actions of angiotensin II main effector molecule synthesized by angiotensin converting enzyme (ACE). Opposite influences of angiotensin II (AngII) receptor type 1 (AT1R) and angiotensin II receptor type 2 (ATR2) on sympathetic tone have been documented. Genetic studies have shown that individuals with VVS usually have a positive family history. Still, the genetic basis of VVS is unclear. The aim of the study was to investigate the association of the RAS gene variants, ACE I/D, AT1R A1166C and ATR2 -1332 A/G with the VVS in Serbian population. Methods: This case-control designed study included 215 VVS patients and 439 controls (C) matched by age (mean±SD, VVS: 38,25±13,52 years and C: 36,63±11,69 years). Genotyping was done by PCR-RFLP and allele specific PCR methods. Results: There were no significant differences in the genotype frequency distributions of ACE I/D and AT1R A1166C variants between cases and controls (p=0.4 and p=0.2, respectively). With regard to X-linked ATR2 -1332 A/G variant the frequency of A/- hemizygotes was significantly higher in VVS than in controls, in males (p=0.01). In females there were no differences in genotype frequency distributions between cases and controls (p=0.73). Conclusions: Our results suggest further investigation of AT2R hemodynamic effect on AngII in VVS males. To accurately elucidate given association, replication of the results in a larger sample is inevitable.",
journal = "ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts",
title = "Renin-angiotensin system gene variants in association with vasovagal syncope in Serbian population",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12682"
}

Životić, I., Kolić, I., Đurić, T., Živković, M., Milovanović, B.,& Stanković, A.. (2019). Renin-angiotensin system gene variants in association with vasovagal syncope in Serbian population. in ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts.
https://hdl.handle.net/21.15107/rcub_vinar_12682

Životić I, Kolić I, Đurić T, Živković M, Milovanović B, Stanković A. Renin-angiotensin system gene variants in association with vasovagal syncope in Serbian population. in ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts. 2019;.
https://hdl.handle.net/21.15107/rcub_vinar_12682 .

Životić, Ivan, Kolić, Ivana, Đurić, Tamara, Živković, Maja, Milovanović, Branislav, Stanković, Aleksandra, "Renin-angiotensin system gene variants in association with vasovagal syncope in Serbian population" in ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts (2019),
https://hdl.handle.net/21.15107/rcub_vinar_12682 .

TY  - CONF
AU  - Kolić, Ivana
AU  - Životić, Ivan
AU  - Đurić, Tamara
AU  - Živković, Maja
AU  - Alavantić, Dragan
AU  - Jovanović, Dušica
AU  - Milovanović, Branislav
AU  - Stanković, Aleksandra
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12683
AB  - Background: Disturbance in sympathovagal balance were strongly associated withoccurrence of syncope.The renin-angiotensin system (RAS) interacts with the autonomous nervous system (ANS) in the regulation of blood pressure and cardiovascular function. By now, several genetic variants in the RAS have been identified as factors in alteration of HRV parameters, haemodynamic values,heart rate, and BP.The aim of our study was to investigate the association of ACE I/D, AGTR1 +1166A/C and AGTR2 -1332A/G gene variants and ANS function. Methods: This study included 215 syncope patients (age (mean±SD)=38.15±13.52 years, 79% females and 21% males) whose ANS function was evaluated by power spectral analysis of heart rate variability (HRV) before and during Tilt test (TT). Genotyping was done by PCR-RFLP and allele specific PCR methods. Statistical analysis was performed using STATISTICA data analysis software system (StatSoft, Inc. (2007). STATISTICA, version 8.0. www.statsoft.com). Results: There were no significant associations of ACE I/D or AGTR2 -1332A/G gene variants with the level of HRV parameters. We found that homozygotic carriers of both AGTR1 +1166A/C alleles have significantly increased LF component in supine position before TT, compared to heterozygote carriers (p=0,04, Mann-Whitney U test). During the TT there were no significant diferences in level of LF component with regard to AGTR1 +1166A/C genotypes. Conclusions: The present study suggest association of AGTR1 +1166A/C variant with LF component adrressing predisposition to syncopal event during TT. This association need to be confirmed in further genetic association studies on a larger sample.
C3  - ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts
T1  - Association of ACE I/D, AGTR1 +1166A/C and AGTR2 -1332A/G gene variants with autonomous nervous system function in Serbian syncope patients
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12683
ER  - 

@conference{
author = "Kolić, Ivana and Životić, Ivan and Đurić, Tamara and Živković, Maja and Alavantić, Dragan and Jovanović, Dušica and Milovanović, Branislav and Stanković, Aleksandra",
year = "2019",
abstract = "Background: Disturbance in sympathovagal balance were strongly associated withoccurrence of syncope.The renin-angiotensin system (RAS) interacts with the autonomous nervous system (ANS) in the regulation of blood pressure and cardiovascular function. By now, several genetic variants in the RAS have been identified as factors in alteration of HRV parameters, haemodynamic values,heart rate, and BP.The aim of our study was to investigate the association of ACE I/D, AGTR1 +1166A/C and AGTR2 -1332A/G gene variants and ANS function. Methods: This study included 215 syncope patients (age (mean±SD)=38.15±13.52 years, 79% females and 21% males) whose ANS function was evaluated by power spectral analysis of heart rate variability (HRV) before and during Tilt test (TT). Genotyping was done by PCR-RFLP and allele specific PCR methods. Statistical analysis was performed using STATISTICA data analysis software system (StatSoft, Inc. (2007). STATISTICA, version 8.0. www.statsoft.com). Results: There were no significant associations of ACE I/D or AGTR2 -1332A/G gene variants with the level of HRV parameters. We found that homozygotic carriers of both AGTR1 +1166A/C alleles have significantly increased LF component in supine position before TT, compared to heterozygote carriers (p=0,04, Mann-Whitney U test). During the TT there were no significant diferences in level of LF component with regard to AGTR1 +1166A/C genotypes. Conclusions: The present study suggest association of AGTR1 +1166A/C variant with LF component adrressing predisposition to syncopal event during TT. This association need to be confirmed in further genetic association studies on a larger sample.",
journal = "ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts",
title = "Association of ACE I/D, AGTR1 +1166A/C and AGTR2 -1332A/G gene variants with autonomous nervous system function in Serbian syncope patients",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12683"
}

Kolić, I., Životić, I., Đurić, T., Živković, M., Alavantić, D., Jovanović, D., Milovanović, B.,& Stanković, A.. (2019). Association of ACE I/D, AGTR1 +1166A/C and AGTR2 -1332A/G gene variants with autonomous nervous system function in Serbian syncope patients. in ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts.
https://hdl.handle.net/21.15107/rcub_vinar_12683

Kolić I, Životić I, Đurić T, Živković M, Alavantić D, Jovanović D, Milovanović B, Stanković A. Association of ACE I/D, AGTR1 +1166A/C and AGTR2 -1332A/G gene variants with autonomous nervous system function in Serbian syncope patients. in ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts. 2019;.
https://hdl.handle.net/21.15107/rcub_vinar_12683 .

Kolić, Ivana, Životić, Ivan, Đurić, Tamara, Živković, Maja, Alavantić, Dragan, Jovanović, Dušica, Milovanović, Branislav, Stanković, Aleksandra, "Association of ACE I/D, AGTR1 +1166A/C and AGTR2 -1332A/G gene variants with autonomous nervous system function in Serbian syncope patients" in ICE 2019 : International Congress on Electrocardiology : Joint Meeting of ISHNE and ISE : Book of abstracts (2019),
https://hdl.handle.net/21.15107/rcub_vinar_12683 .

TY  - JOUR
AU  - Životić, Ivan
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Milašinović, Dejan
AU  - Stanković, Goran
AU  - Dekleva, Milica
AU  - Marković-Nikolić, Nataša
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8432
AB  - Background: Chromosomal region 9p21.3 is most robustly associated with coronary artery disease (CAD) in western European populations. However, heterogeneity in CAD phenotypes leads to uncertainty whether 9p21.3 is associated with stable and/or acute clinical presentations of CAD. 9p21.3 is rich in regulatory elements, but the underlying mechanisms of its actions in CAD remain unclear. We investigate the association of 9p21.3 two haplotype blocks lead variants (rs10757278 and rs518394) with first-ever non-fatal myocardial infarction (MI) in CAD patients and their association with CDKN2B mRNA expression in peripheral blood mononuclear cells 6 months after the event. Methods: We included CAD patients with sustained first MI (n = 523) and controls (n = 583). Gene expression was assessed in 72 patients 6 months after MI and 43 healthy controls. TaqMan® technology was used for the gene expression and genotyping analysis. Results: CDKN2B mRNA was significantly lower in MI patients compared with the controls (p = 0.002) and in patients carrying the rs10757278 G risk allele versus AA homozygotes (p = 0.012) 6 months after the event. While we confirmed the association of rs10757278 with CDKN2B expression in MI patients, we failed to find an association between the investigated variants and MI or disease burden. Conclusions: We suggest a dysregulation of gene expression in the 9p21.3 region six months after acute MI, which is affected by a genetic variant in patients. The rs10757278 rare allele is one factor that might lead to prolonged risk for proatherogenic complications. © 2019 The Canadian Society of Clinical Chemists
T2  - Clinical Biochemistry
T1  - CDKN2B gene expression is affected by 9p21.3 rs10757278 in CAD patients, six months after the MI
VL  - 73
SP  - 70
EP  - 76
DO  - 10.1016/j.clinbiochem.2019.08.003
ER  - 

@article{
author = "Životić, Ivan and Đurić, Tamara and Stanković, Aleksandra and Milašinović, Dejan and Stanković, Goran and Dekleva, Milica and Marković-Nikolić, Nataša and Alavantić, Dragan and Živković, Maja",
year = "2019",
abstract = "Background: Chromosomal region 9p21.3 is most robustly associated with coronary artery disease (CAD) in western European populations. However, heterogeneity in CAD phenotypes leads to uncertainty whether 9p21.3 is associated with stable and/or acute clinical presentations of CAD. 9p21.3 is rich in regulatory elements, but the underlying mechanisms of its actions in CAD remain unclear. We investigate the association of 9p21.3 two haplotype blocks lead variants (rs10757278 and rs518394) with first-ever non-fatal myocardial infarction (MI) in CAD patients and their association with CDKN2B mRNA expression in peripheral blood mononuclear cells 6 months after the event. Methods: We included CAD patients with sustained first MI (n = 523) and controls (n = 583). Gene expression was assessed in 72 patients 6 months after MI and 43 healthy controls. TaqMan® technology was used for the gene expression and genotyping analysis. Results: CDKN2B mRNA was significantly lower in MI patients compared with the controls (p = 0.002) and in patients carrying the rs10757278 G risk allele versus AA homozygotes (p = 0.012) 6 months after the event. While we confirmed the association of rs10757278 with CDKN2B expression in MI patients, we failed to find an association between the investigated variants and MI or disease burden. Conclusions: We suggest a dysregulation of gene expression in the 9p21.3 region six months after acute MI, which is affected by a genetic variant in patients. The rs10757278 rare allele is one factor that might lead to prolonged risk for proatherogenic complications. © 2019 The Canadian Society of Clinical Chemists",
journal = "Clinical Biochemistry",
title = "CDKN2B gene expression is affected by 9p21.3 rs10757278 in CAD patients, six months after the MI",
volume = "73",
pages = "70-76",
doi = "10.1016/j.clinbiochem.2019.08.003"
}

Životić, I., Đurić, T., Stanković, A., Milašinović, D., Stanković, G., Dekleva, M., Marković-Nikolić, N., Alavantić, D.,& Živković, M.. (2019). CDKN2B gene expression is affected by 9p21.3 rs10757278 in CAD patients, six months after the MI. in Clinical Biochemistry, 73, 70-76.
https://doi.org/10.1016/j.clinbiochem.2019.08.003

Životić I, Đurić T, Stanković A, Milašinović D, Stanković G, Dekleva M, Marković-Nikolić N, Alavantić D, Živković M. CDKN2B gene expression is affected by 9p21.3 rs10757278 in CAD patients, six months after the MI. in Clinical Biochemistry. 2019;73:70-76.
doi:10.1016/j.clinbiochem.2019.08.003 .

Životić, Ivan, Đurić, Tamara, Stanković, Aleksandra, Milašinović, Dejan, Stanković, Goran, Dekleva, Milica, Marković-Nikolić, Nataša, Alavantić, Dragan, Živković, Maja, "CDKN2B gene expression is affected by 9p21.3 rs10757278 in CAD patients, six months after the MI" in Clinical Biochemistry, 73 (2019):70-76,
https://doi.org/10.1016/j.clinbiochem.2019.08.003 . .

TY  - THES
AU  - Životić, Ivan
PY  - 2019
UR  - http://nardus.mpn.gov.rs/handle/123456789/11534
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=6931
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:20301/bdef:Content/download
UR  - https://plus.sr.cobiss.net/opac7/bib/1025217714
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8727
AB  - Ateroskleroza je kompleksna, hronična i progresivna bolest arterijskih krvnih sudova koja se razvija tokom života i najčešće se klinički manifestuje u kasnijem životnom dobu. Dve najznačajnije kliničke komplikacije su ishemijski moždni i srčani udar (IM), na čiji nastanak utiču sredinski i nasledni faktori (genetska predispozicija). U ovoj studiji ispitana je asocijacija četiri genetičke varijante prisutne u hromozomskom regionu 9p21 (rs10757278 i rs518394 u genu ANRIL, i rs36212560 i rs2275888 u genu HACD4), sa rizikom za nastanak IM, karotidnog plaka (KP) i kliničkih komplikacija KP (ultrasonografski definisan tip plaka, simptomatska karotidna bolest, prisustvo ulceracije na plaku). Ispitana je transkripcija gena CDKN2B i HACD4 kao i nivo iRNK u tkivu karotidnog aterosklerotskog plaka i mononuklearnim ćelijama periferne krvi (MĆPK) bolesnika koji su doživeli prvi IM, 6 meseci nakon infarkta. Nivo iRNK ispitan je u odnosu na genotipove i tip karotidnog plaka.Utvrđena je značajna asocijacija rs10757278 sa rizikom za nastanak KP kod žena. Utvrđeno je da je haplotip genetičkih varijanti rs36212560 i rs2275888 značajno asociran sa rizikom za nastanak KP i IM. Rezutlat je pokazao polno specifičnu asocijaciju (kod muškaraca) kao i značajnu asocijaciju varijante rs36212560 testirane van haplotipa. Nije detektovana značajna asocijacija genetičkih varijanti rs2275888 i rs518394 sa rizikom za razvoj karotidnog plaka, ali je ustanovljena značajna, nezavisna asocijacija varijante rs2275888 sa rizikom za nastanak IM. Varijanta rs10757278 je značajno asocirana sa tipom karotidnog aterosklerotskog plaka. Ustanovljeno je da je haplotip varijanti rs36212560 i rs2275888 značajno asociran sa rizikom za razvoj ulceracije karotidnog plaka, i sa simptomatskom karotidnom bolešću u grupi bolesnika sa KP, kao i da polno specifična asocijacija sa haplotipom postoji kod muškaraca.Razlika u nivoima ekspresije merena je na nivou iRNK u odnosu na genotipove ispitivanih varijanti. U tkivu KP nije detektovana razlika u nivou iRNK za CDKN2B u odnosu na genotipove ispitivanih varijanti, ali je ustanovljen statistički trend u asocijaciji sa genotipom varijante rs1075278. Varijanta rs2275888 je prethodno okarakterisan kao eQTL (eng. expression Quantitative Trait Locus) za gen FOCAD gen u tibijalnoj arteriji...
AB  - Atherosclerosis is a complex, chronic and progeressive disease of arterial blood vessels that develops during the life and usualy with clinical manifestations at later age. Two most singnifficant clinical manifestations are stroke and myocardial infarction (MI), which development is affected by the median and hereditary factors (genetic predisposition). In this study, association of four genetic variants from chromosome region 9p21 have been investigated (rs10757278 and rs518394 in the ANRIL gene, and rs36212560 and rs2275888 in the HACD4 gene) with the risk for development of MI, the carotid plaque (CP) and its clinical complications (ultrasonographicaly deffined plaque type, symphtomatic disease, presence of carotid plaque ulceration). The transcription of genes CDKN2B and HACD4 at the mRNA level was examined in the CP tissue and peripheral blood mononuclear cells (PBMC) of patients who sustained first MI, six months after the infarction. Level of mRNA was examined in relation to genotypes and CP type.Signifficant association was determined for rs10757278 with the risk for CP development among females. It was determined that haplotype of gene variants rs36212560 and rs2275888 is significantly associated with the risk for CP and MI development. Result has shown gender specific association (among males) as well as association of rs36212560 with CP and MI when tested independently. It wasn’t detected signifficant association of gene variants rs2275888 and rs518394 with the risk for the development of CP, but, the signifficant idependant association of rs2275888 variant with the risk for MI. Variant rs10757278 is signifficantly associated with the carotid atherosclerotic plaque type. Haplotype of rs36212560 and rs2275888 variants is signifficantly associated with the carotid plaque ulceration, as well as with simpthomatic carotid disease in the group of CP patients., and in addition, the gender speciffic association with the haplotype exist in males.Diference in expression levels was measured at the level of mRNA in relation to genotypes of tested variants. In CP tissue difference in CDKN2B mRNK level wasn’t detected in relation to tested variants according to genotype, but statistical trend was detected in association with rs10757278 genotypes...
PB  - Универзитет у Београду, Биолошки факултет
T2  - Универзитет у Београду
T1  - Asocijacija genskih varijanti na hromozomu 9p21 i transkripcije gena za CDKN2B i HACD4 sa nastankom ateroskleroze i njenim kliničkim komplikacijama kod čoveka
UR  - https://hdl.handle.net/21.15107/rcub_nardus_11534
ER  - 

@phdthesis{
author = "Životić, Ivan",
year = "2019",
abstract = "Ateroskleroza je kompleksna, hronična i progresivna bolest arterijskih krvnih sudova koja se razvija tokom života i najčešće se klinički manifestuje u kasnijem životnom dobu. Dve najznačajnije kliničke komplikacije su ishemijski moždni i srčani udar (IM), na čiji nastanak utiču sredinski i nasledni faktori (genetska predispozicija). U ovoj studiji ispitana je asocijacija četiri genetičke varijante prisutne u hromozomskom regionu 9p21 (rs10757278 i rs518394 u genu ANRIL, i rs36212560 i rs2275888 u genu HACD4), sa rizikom za nastanak IM, karotidnog plaka (KP) i kliničkih komplikacija KP (ultrasonografski definisan tip plaka, simptomatska karotidna bolest, prisustvo ulceracije na plaku). Ispitana je transkripcija gena CDKN2B i HACD4 kao i nivo iRNK u tkivu karotidnog aterosklerotskog plaka i mononuklearnim ćelijama periferne krvi (MĆPK) bolesnika koji su doživeli prvi IM, 6 meseci nakon infarkta. Nivo iRNK ispitan je u odnosu na genotipove i tip karotidnog plaka.Utvrđena je značajna asocijacija rs10757278 sa rizikom za nastanak KP kod žena. Utvrđeno je da je haplotip genetičkih varijanti rs36212560 i rs2275888 značajno asociran sa rizikom za nastanak KP i IM. Rezutlat je pokazao polno specifičnu asocijaciju (kod muškaraca) kao i značajnu asocijaciju varijante rs36212560 testirane van haplotipa. Nije detektovana značajna asocijacija genetičkih varijanti rs2275888 i rs518394 sa rizikom za razvoj karotidnog plaka, ali je ustanovljena značajna, nezavisna asocijacija varijante rs2275888 sa rizikom za nastanak IM. Varijanta rs10757278 je značajno asocirana sa tipom karotidnog aterosklerotskog plaka. Ustanovljeno je da je haplotip varijanti rs36212560 i rs2275888 značajno asociran sa rizikom za razvoj ulceracije karotidnog plaka, i sa simptomatskom karotidnom bolešću u grupi bolesnika sa KP, kao i da polno specifična asocijacija sa haplotipom postoji kod muškaraca.Razlika u nivoima ekspresije merena je na nivou iRNK u odnosu na genotipove ispitivanih varijanti. U tkivu KP nije detektovana razlika u nivou iRNK za CDKN2B u odnosu na genotipove ispitivanih varijanti, ali je ustanovljen statistički trend u asocijaciji sa genotipom varijante rs1075278. Varijanta rs2275888 je prethodno okarakterisan kao eQTL (eng. expression Quantitative Trait Locus) za gen FOCAD gen u tibijalnoj arteriji..., Atherosclerosis is a complex, chronic and progeressive disease of arterial blood vessels that develops during the life and usualy with clinical manifestations at later age. Two most singnifficant clinical manifestations are stroke and myocardial infarction (MI), which development is affected by the median and hereditary factors (genetic predisposition). In this study, association of four genetic variants from chromosome region 9p21 have been investigated (rs10757278 and rs518394 in the ANRIL gene, and rs36212560 and rs2275888 in the HACD4 gene) with the risk for development of MI, the carotid plaque (CP) and its clinical complications (ultrasonographicaly deffined plaque type, symphtomatic disease, presence of carotid plaque ulceration). The transcription of genes CDKN2B and HACD4 at the mRNA level was examined in the CP tissue and peripheral blood mononuclear cells (PBMC) of patients who sustained first MI, six months after the infarction. Level of mRNA was examined in relation to genotypes and CP type.Signifficant association was determined for rs10757278 with the risk for CP development among females. It was determined that haplotype of gene variants rs36212560 and rs2275888 is significantly associated with the risk for CP and MI development. Result has shown gender specific association (among males) as well as association of rs36212560 with CP and MI when tested independently. It wasn’t detected signifficant association of gene variants rs2275888 and rs518394 with the risk for the development of CP, but, the signifficant idependant association of rs2275888 variant with the risk for MI. Variant rs10757278 is signifficantly associated with the carotid atherosclerotic plaque type. Haplotype of rs36212560 and rs2275888 variants is signifficantly associated with the carotid plaque ulceration, as well as with simpthomatic carotid disease in the group of CP patients., and in addition, the gender speciffic association with the haplotype exist in males.Diference in expression levels was measured at the level of mRNA in relation to genotypes of tested variants. In CP tissue difference in CDKN2B mRNK level wasn’t detected in relation to tested variants according to genotype, but statistical trend was detected in association with rs10757278 genotypes...",
publisher = "Универзитет у Београду, Биолошки факултет",
journal = "Универзитет у Београду",
title = "Asocijacija genskih varijanti na hromozomu 9p21 i transkripcije gena za CDKN2B i HACD4 sa nastankom ateroskleroze i njenim kliničkim komplikacijama kod čoveka",
url = "https://hdl.handle.net/21.15107/rcub_nardus_11534"
}

Životić, I.. (2019). Asocijacija genskih varijanti na hromozomu 9p21 i transkripcije gena za CDKN2B i HACD4 sa nastankom ateroskleroze i njenim kliničkim komplikacijama kod čoveka. in Универзитет у Београду
Универзитет у Београду, Биолошки факултет..
https://hdl.handle.net/21.15107/rcub_nardus_11534

Životić I. Asocijacija genskih varijanti na hromozomu 9p21 i transkripcije gena za CDKN2B i HACD4 sa nastankom ateroskleroze i njenim kliničkim komplikacijama kod čoveka. in Универзитет у Београду. 2019;.
https://hdl.handle.net/21.15107/rcub_nardus_11534 .

Životić, Ivan, "Asocijacija genskih varijanti na hromozomu 9p21 i transkripcije gena za CDKN2B i HACD4 sa nastankom ateroskleroze i njenim kliničkim komplikacijama kod čoveka" in Универзитет у Београду (2019),
https://hdl.handle.net/21.15107/rcub_nardus_11534 .

TY  - JOUR
AU  - Životić, Ivan
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Ivancevic, Ilija
AU  - Končar, Igor
AU  - Milašinović, Dejan
AU  - Stanković, Goran
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1843
AB  - The 9p21.3 region is rich in regulatory elements and the variants in this region had been robustly associated with carotid plaque (CP) and coronary artery disease (CAD). Recently, the HACD4 was detected as one of the six 9p21.3 differentially expressed genes associated with accelerated atherosclerosis and greater mean lesion area in the Athsq1 congenic mice. We aimed to investigate association of two potentially regulatory HACD4 variants (rs36212560 I/D, rs2275888 T/C) and their haplotypes with CP occurrence and the level of HACD4 and FOCAD mRNA in human CP tissue. Association study was replicated in CAD patients who suffered the first myocardial infarction. Study included 477 CP patients, 303 healthy controls and replication sample of 224 CAD males from the population of Serbia. Genotypes were determined by polymerase chain reaction (PCR) and real-time PCR using TaqMan (R) technology. The gene expression was detected with TaqMan (R) technology. We have found significant and independent association of DT haplotype with CP presence in men (adjusted OR = 1.64 CI = 1.12-2.42, p = 0.011). The result was replicated in CAD males (adjusted OR = 1.84 CI = 1.21-2.80, p = 0.004). We have found significant effect of the HACD4 rs2275888 on FOCAD mRNA level in human CP tissue. Correction for multiple testing was performed. Independent association of HACD4 haplotypes with atherosclerotic phenotypes connotes a further validation and replication in larger cohorts as well as functional studies to enlighten the potential mechanism of its action in pathophysiology of atherosclerosis.
T2  - Gene
T1  - The HACD4 haplotype as a risk factor for atherosclerosis in males
VL  - 641
SP  - 35
EP  - 40
DO  - 10.1016/j.gene.2017.10.030
ER  - 

@article{
author = "Životić, Ivan and Đurić, Tamara and Stanković, Aleksandra and Ivancevic, Ilija and Končar, Igor and Milašinović, Dejan and Stanković, Goran and Alavantić, Dragan and Živković, Maja",
year = "2018",
abstract = "The 9p21.3 region is rich in regulatory elements and the variants in this region had been robustly associated with carotid plaque (CP) and coronary artery disease (CAD). Recently, the HACD4 was detected as one of the six 9p21.3 differentially expressed genes associated with accelerated atherosclerosis and greater mean lesion area in the Athsq1 congenic mice. We aimed to investigate association of two potentially regulatory HACD4 variants (rs36212560 I/D, rs2275888 T/C) and their haplotypes with CP occurrence and the level of HACD4 and FOCAD mRNA in human CP tissue. Association study was replicated in CAD patients who suffered the first myocardial infarction. Study included 477 CP patients, 303 healthy controls and replication sample of 224 CAD males from the population of Serbia. Genotypes were determined by polymerase chain reaction (PCR) and real-time PCR using TaqMan (R) technology. The gene expression was detected with TaqMan (R) technology. We have found significant and independent association of DT haplotype with CP presence in men (adjusted OR = 1.64 CI = 1.12-2.42, p = 0.011). The result was replicated in CAD males (adjusted OR = 1.84 CI = 1.21-2.80, p = 0.004). We have found significant effect of the HACD4 rs2275888 on FOCAD mRNA level in human CP tissue. Correction for multiple testing was performed. Independent association of HACD4 haplotypes with atherosclerotic phenotypes connotes a further validation and replication in larger cohorts as well as functional studies to enlighten the potential mechanism of its action in pathophysiology of atherosclerosis.",
journal = "Gene",
title = "The HACD4 haplotype as a risk factor for atherosclerosis in males",
volume = "641",
pages = "35-40",
doi = "10.1016/j.gene.2017.10.030"
}

Životić, I., Đurić, T., Stanković, A., Ivancevic, I., Končar, I., Milašinović, D., Stanković, G., Alavantić, D.,& Živković, M.. (2018). The HACD4 haplotype as a risk factor for atherosclerosis in males. in Gene, 641, 35-40.
https://doi.org/10.1016/j.gene.2017.10.030

Životić I, Đurić T, Stanković A, Ivancevic I, Končar I, Milašinović D, Stanković G, Alavantić D, Živković M. The HACD4 haplotype as a risk factor for atherosclerosis in males. in Gene. 2018;641:35-40.
doi:10.1016/j.gene.2017.10.030 .

Životić, Ivan, Đurić, Tamara, Stanković, Aleksandra, Ivancevic, Ilija, Končar, Igor, Milašinović, Dejan, Stanković, Goran, Alavantić, Dragan, Živković, Maja, "The HACD4 haplotype as a risk factor for atherosclerosis in males" in Gene, 641 (2018):35-40,
https://doi.org/10.1016/j.gene.2017.10.030 . .

TY  - CONF
AU  - Životić, Ivan
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Stanković, Goran
AU  - Milašinović, Dejan
AU  - Dekleva, Milica
AU  - Marković-Nikolić, Nataša
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2018
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0021915018309699
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7953
AB  - Aim: Myocardial infarction (MI) incidence is still a major burden in the
terms of premature death and disability world-wide. Genetic studies up to
date explained a limited portion of disease inheritance. Recently, the DT
haplotype of variants rs36212560 I/D (insertion/deletion) and 2275888 T/C
in HACD gene (9p21.3) have been significantly associated with the risk of
carotid plaque occurrence among males.
Methods: We aimed to validate these results by investigating 518 MI patients (366 males) and 303 (167 males) healthy controls from Serbia. Also, he HACD4 gene expression analysis has been conducted in the peripheral
blood mononuclear cells of 72 MI patients, 6 months after the MI. Results
were obtained using ABI-Prism 7500 RT-PCR for rs2275888 allelic
discrimination and HACD4 mRNA relative quantitation. PCR and acrylamide gel electrophoresis were used to distinguish 5 base pairs
rs36212560 insertion deletion polymorphisms.
Results: Haplotype analysis (using Thesias software) showed that DT
haplotype carriers had significantly higher risk for MI (OR ¼ 1.42, CI 1.08-
1.85, p¼0.01) compared with most frequent IT haplotype. In gender
separated groups association remained significant only among males (OR
¼ 1.76, 1.266-2.46, p¼0.0008). Results were adjusted for MI traditional risk
factors (Age, BMI, HDLC, LDLC, Tg, hypertension and smoking). Investigated
genetic variants were not associated with HACD4 expression. Significant
correlation was found between HACD4 mRNA level and age (r¼0.36,
p¼0.001).
Conclusions: We have shown that rs36212560 and rs2275888 DT haplotype from HACD4 gene is significantly and independently associated with
the MI occurrence in males. Additional studies are needed to confirm these
results.
C3  - Atherosclerosis
T1  - HACD4 haplotype confers risk of myocardial infarction among males in the population of Serbia
VL  - 275
SP  - e210
EP  - e211
DO  - 10.1016/j.atherosclerosis.2018.06.657
ER  - 

@conference{
author = "Životić, Ivan and Đurić, Tamara and Stanković, Aleksandra and Stanković, Goran and Milašinović, Dejan and Dekleva, Milica and Marković-Nikolić, Nataša and Alavantić, Dragan and Živković, Maja",
year = "2018",
abstract = "Aim: Myocardial infarction (MI) incidence is still a major burden in the
terms of premature death and disability world-wide. Genetic studies up to
date explained a limited portion of disease inheritance. Recently, the DT
haplotype of variants rs36212560 I/D (insertion/deletion) and 2275888 T/C
in HACD gene (9p21.3) have been significantly associated with the risk of
carotid plaque occurrence among males.
Methods: We aimed to validate these results by investigating 518 MI patients (366 males) and 303 (167 males) healthy controls from Serbia. Also, he HACD4 gene expression analysis has been conducted in the peripheral
blood mononuclear cells of 72 MI patients, 6 months after the MI. Results
were obtained using ABI-Prism 7500 RT-PCR for rs2275888 allelic
discrimination and HACD4 mRNA relative quantitation. PCR and acrylamide gel electrophoresis were used to distinguish 5 base pairs
rs36212560 insertion deletion polymorphisms.
Results: Haplotype analysis (using Thesias software) showed that DT
haplotype carriers had significantly higher risk for MI (OR ¼ 1.42, CI 1.08-
1.85, p¼0.01) compared with most frequent IT haplotype. In gender
separated groups association remained significant only among males (OR
¼ 1.76, 1.266-2.46, p¼0.0008). Results were adjusted for MI traditional risk
factors (Age, BMI, HDLC, LDLC, Tg, hypertension and smoking). Investigated
genetic variants were not associated with HACD4 expression. Significant
correlation was found between HACD4 mRNA level and age (r¼0.36,
p¼0.001).
Conclusions: We have shown that rs36212560 and rs2275888 DT haplotype from HACD4 gene is significantly and independently associated with
the MI occurrence in males. Additional studies are needed to confirm these
results.",
journal = "Atherosclerosis",
title = "HACD4 haplotype confers risk of myocardial infarction among males in the population of Serbia",
volume = "275",
pages = "e210-e211",
doi = "10.1016/j.atherosclerosis.2018.06.657"
}

Životić, I., Đurić, T., Stanković, A., Stanković, G., Milašinović, D., Dekleva, M., Marković-Nikolić, N., Alavantić, D.,& Živković, M.. (2018). HACD4 haplotype confers risk of myocardial infarction among males in the population of Serbia. in Atherosclerosis, 275, e210-e211.
https://doi.org/10.1016/j.atherosclerosis.2018.06.657

Životić I, Đurić T, Stanković A, Stanković G, Milašinović D, Dekleva M, Marković-Nikolić N, Alavantić D, Živković M. HACD4 haplotype confers risk of myocardial infarction among males in the population of Serbia. in Atherosclerosis. 2018;275:e210-e211.
doi:10.1016/j.atherosclerosis.2018.06.657 .

Životić, Ivan, Đurić, Tamara, Stanković, Aleksandra, Stanković, Goran, Milašinović, Dejan, Dekleva, Milica, Marković-Nikolić, Nataša, Alavantić, Dragan, Živković, Maja, "HACD4 haplotype confers risk of myocardial infarction among males in the population of Serbia" in Atherosclerosis, 275 (2018):e210-e211,
https://doi.org/10.1016/j.atherosclerosis.2018.06.657 . .

TY  - CONF
AU  - Životić, Ivan
AU  - Jovanović, Ivan
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Dekleva, Milica
AU  - Marković Nikolić, Nevena
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12816
AB  - Introduction: Recently, rs2275888 eQTL in PTPLAD2 gene has been associated with expression of several loci, during inflammatory stimulation in monocytes. Myocardial infarction (MI) triggers an intense inflammatory response that is essential for cardiac repair. We aimed to perform data scouting in appropriate rs2275888 genotype model to identify differentially expressed genes (DEGs), their biological meaning, and key miRs potentially associated with rs2275888 eQTL in peripheral blood mononuclear leucocytes (PBML) of MI patients 6 months after first MI. Methods: Transcriptome data was obtained from PBMLs of 21 patients, who suffered ischemic MI, by employing Illumina iScan microarray technology. Genotyping for rs2275888 was conducted with real-time PCR, using TaqMan® assay. Preprocessing and identification of DEGs was done using limma package of R/Bioconductor software. The online tool DAVID v6.8 was employed for functional enrichment analysis. Most important miRs were selected using NetworkAnalyst web tool, based on the degree centrality value. Results: Transcriptome analysis in recessive model TT+TC (n=19) vs. CC (n=2) identified 68 DEGs. Top significant biological processes involving DEGs cover vascular physiology, cell growth and signaling. Pathway analysis associated DEGs with adherens junction, Rap1 and Ras signaling. Network analysis identified hsa-miR335-5p, -26b-5p, -93-5p, -16-5p, -124-3p, -20b-5p, -17-5p and -218-5p as miRs with top centrality degree in our DEGs list. Conclusion: Seven of eight detected miRs have already been described in MI pathology or suggested as potential biomarkers for MI. Our result suggest the importance of integration of eQTLs, biological processes and pathway analysis coupled with miR activity for further research in MI pathology.
PB  - Belgrade : University of Belgrade, Faculty of Biology
C3  - CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts
T1  - Microarray transcriptome profiling in myocardial infarction regarding PTPLAD2 rs2275888 eQTL: a data scouting approach
SP  - 176
EP  - 176
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12816
ER  - 

@conference{
author = "Životić, Ivan and Jovanović, Ivan and Đurić, Tamara and Stanković, Aleksandra and Dekleva, Milica and Marković Nikolić, Nevena and Alavantić, Dragan and Živković, Maja",
year = "2017",
abstract = "Introduction: Recently, rs2275888 eQTL in PTPLAD2 gene has been associated with expression of several loci, during inflammatory stimulation in monocytes. Myocardial infarction (MI) triggers an intense inflammatory response that is essential for cardiac repair. We aimed to perform data scouting in appropriate rs2275888 genotype model to identify differentially expressed genes (DEGs), their biological meaning, and key miRs potentially associated with rs2275888 eQTL in peripheral blood mononuclear leucocytes (PBML) of MI patients 6 months after first MI. Methods: Transcriptome data was obtained from PBMLs of 21 patients, who suffered ischemic MI, by employing Illumina iScan microarray technology. Genotyping for rs2275888 was conducted with real-time PCR, using TaqMan® assay. Preprocessing and identification of DEGs was done using limma package of R/Bioconductor software. The online tool DAVID v6.8 was employed for functional enrichment analysis. Most important miRs were selected using NetworkAnalyst web tool, based on the degree centrality value. Results: Transcriptome analysis in recessive model TT+TC (n=19) vs. CC (n=2) identified 68 DEGs. Top significant biological processes involving DEGs cover vascular physiology, cell growth and signaling. Pathway analysis associated DEGs with adherens junction, Rap1 and Ras signaling. Network analysis identified hsa-miR335-5p, -26b-5p, -93-5p, -16-5p, -124-3p, -20b-5p, -17-5p and -218-5p as miRs with top centrality degree in our DEGs list. Conclusion: Seven of eight detected miRs have already been described in MI pathology or suggested as potential biomarkers for MI. Our result suggest the importance of integration of eQTLs, biological processes and pathway analysis coupled with miR activity for further research in MI pathology.",
publisher = "Belgrade : University of Belgrade, Faculty of Biology",
journal = "CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts",
title = "Microarray transcriptome profiling in myocardial infarction regarding PTPLAD2 rs2275888 eQTL: a data scouting approach",
pages = "176-176",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12816"
}

Životić, I., Jovanović, I., Đurić, T., Stanković, A., Dekleva, M., Marković Nikolić, N., Alavantić, D.,& Živković, M.. (2017). Microarray transcriptome profiling in myocardial infarction regarding PTPLAD2 rs2275888 eQTL: a data scouting approach. in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts
Belgrade : University of Belgrade, Faculty of Biology., 176-176.
https://hdl.handle.net/21.15107/rcub_vinar_12816

Životić I, Jovanović I, Đurić T, Stanković A, Dekleva M, Marković Nikolić N, Alavantić D, Živković M. Microarray transcriptome profiling in myocardial infarction regarding PTPLAD2 rs2275888 eQTL: a data scouting approach. in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts. 2017;:176-176.
https://hdl.handle.net/21.15107/rcub_vinar_12816 .

Životić, Ivan, Jovanović, Ivan, Đurić, Tamara, Stanković, Aleksandra, Dekleva, Milica, Marković Nikolić, Nevena, Alavantić, Dragan, Živković, Maja, "Microarray transcriptome profiling in myocardial infarction regarding PTPLAD2 rs2275888 eQTL: a data scouting approach" in CoMBoS1 - 1st Congress of Molecular Biologists of Serbia with international participation : Book of abstracts (2017):176-176,
https://hdl.handle.net/21.15107/rcub_vinar_12816 .

TY  - CONF
AU  - Životić, Ivan
AU  - Živković, Maja
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Đorđević, Ana
AU  - Dekleva, Milica
AU  - Marković-Nikolić, Nevena
AU  - Alavantić, Dragan
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7178
AB  - Aim: Myocardial infarction (MI) is the clinical complication predominately
caused by coronary plaque buildup and rupture during the process of
atherosclerosis. In the 9p21 locus two important risk haplotype blocks
have been identified. The one that carries the risk for MI with the lead
variant rs10757278 and another characterized to influence progression of
the MI, with the lead variant rs518394. We have investigated association of
the two genetic variants with the ST-elevated MI in the gender specific
manner. We have also tested variants effect on p15 mRNA level as one of
the possible mechanisms of the variants effect.Methods: The study group included 147 patients (72 females) with angiographically assessed MI, and 240 healthy controls (90 females). DNA
and RNA (n¼28) where isolated from peripheral blood mono nuclear
leukocytes. Genotypes for rs10757278 A/G and rs518394 C/G, and relative
mRNA level for p15 were determined using commercial TaqMan® assays
on 7500 ABI Real-Time PCR.
Results: We have found significant association of rs10757278 GG with STelevated MI occurrence, with OR of 2.2 (CI¼1.07-4.5, p¼0.03) in females.
P15 mRNA was significantly down-regulated in G allele carriers (AG+GG vs
AA) by a mean factor of 0.449 (S.E. range is 0.188-1.059), p¼0.019 in the
whole group. The genetic variant rs518394 was not significantly associated
either with MI or p15 mRNA level.
Conclusions: Genotype GG of rs10757278 is significantly associated with
MI occurrence in females in Serbian population. On
C3  - Atherosclerosis
T1  - RS10757278 from 9P21 is associated with st-elevated myocardial infarction in females in population of Serbia
VL  - 263
SP  - e188
EP  - e188
DO  - 10.1016/j.atherosclerosis.2017.06.603
ER  - 

@conference{
author = "Životić, Ivan and Živković, Maja and Đurić, Tamara and Stanković, Aleksandra and Đorđević, Ana and Dekleva, Milica and Marković-Nikolić, Nevena and Alavantić, Dragan",
year = "2017",
abstract = "Aim: Myocardial infarction (MI) is the clinical complication predominately
caused by coronary plaque buildup and rupture during the process of
atherosclerosis. In the 9p21 locus two important risk haplotype blocks
have been identified. The one that carries the risk for MI with the lead
variant rs10757278 and another characterized to influence progression of
the MI, with the lead variant rs518394. We have investigated association of
the two genetic variants with the ST-elevated MI in the gender specific
manner. We have also tested variants effect on p15 mRNA level as one of
the possible mechanisms of the variants effect.Methods: The study group included 147 patients (72 females) with angiographically assessed MI, and 240 healthy controls (90 females). DNA
and RNA (n¼28) where isolated from peripheral blood mono nuclear
leukocytes. Genotypes for rs10757278 A/G and rs518394 C/G, and relative
mRNA level for p15 were determined using commercial TaqMan® assays
on 7500 ABI Real-Time PCR.
Results: We have found significant association of rs10757278 GG with STelevated MI occurrence, with OR of 2.2 (CI¼1.07-4.5, p¼0.03) in females.
P15 mRNA was significantly down-regulated in G allele carriers (AG+GG vs
AA) by a mean factor of 0.449 (S.E. range is 0.188-1.059), p¼0.019 in the
whole group. The genetic variant rs518394 was not significantly associated
either with MI or p15 mRNA level.
Conclusions: Genotype GG of rs10757278 is significantly associated with
MI occurrence in females in Serbian population. On",
journal = "Atherosclerosis",
title = "RS10757278 from 9P21 is associated with st-elevated myocardial infarction in females in population of Serbia",
volume = "263",
pages = "e188-e188",
doi = "10.1016/j.atherosclerosis.2017.06.603"
}

Životić, I., Živković, M., Đurić, T., Stanković, A., Đorđević, A., Dekleva, M., Marković-Nikolić, N.,& Alavantić, D.. (2017). RS10757278 from 9P21 is associated with st-elevated myocardial infarction in females in population of Serbia. in Atherosclerosis, 263, e188-e188.
https://doi.org/10.1016/j.atherosclerosis.2017.06.603

Životić I, Živković M, Đurić T, Stanković A, Đorđević A, Dekleva M, Marković-Nikolić N, Alavantić D. RS10757278 from 9P21 is associated with st-elevated myocardial infarction in females in population of Serbia. in Atherosclerosis. 2017;263:e188-e188.
doi:10.1016/j.atherosclerosis.2017.06.603 .

Životić, Ivan, Živković, Maja, Đurić, Tamara, Stanković, Aleksandra, Đorđević, Ana, Dekleva, Milica, Marković-Nikolić, Nevena, Alavantić, Dragan, "RS10757278 from 9P21 is associated with st-elevated myocardial infarction in females in population of Serbia" in Atherosclerosis, 263 (2017):e188-e188,
https://doi.org/10.1016/j.atherosclerosis.2017.06.603 . .

TY  - CONF
AU  - Bošković, Maja
AU  - Bundalo, Maja M.
AU  - Stojiljković, Mojca D.
AU  - Kostić, Milan
AU  - Živković, Maja
AU  - Korićanac, Goran
AU  - Stanković, Aleksandra
AU  - Životić, Ivan
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7179
C3  - Atherosclerosis
T1  - Estradiol Protects Ovariectomized Female Rats Against Fructose-Rich Diet Induced Oxidative Stress
VL  - 263
SP  - E192
EP  - E192
DO  - 10.1016/j.atherosclerosis.2017.06.616
ER  - 

@conference{
author = "Bošković, Maja and Bundalo, Maja M. and Stojiljković, Mojca D. and Kostić, Milan and Živković, Maja and Korićanac, Goran and Stanković, Aleksandra and Životić, Ivan",
year = "2017",
journal = "Atherosclerosis",
title = "Estradiol Protects Ovariectomized Female Rats Against Fructose-Rich Diet Induced Oxidative Stress",
volume = "263",
pages = "E192-E192",
doi = "10.1016/j.atherosclerosis.2017.06.616"
}

Bošković, M., Bundalo, M. M., Stojiljković, M. D., Kostić, M., Živković, M., Korićanac, G., Stanković, A.,& Životić, I.. (2017). Estradiol Protects Ovariectomized Female Rats Against Fructose-Rich Diet Induced Oxidative Stress. in Atherosclerosis, 263, E192-E192.
https://doi.org/10.1016/j.atherosclerosis.2017.06.616

Bošković M, Bundalo MM, Stojiljković MD, Kostić M, Živković M, Korićanac G, Stanković A, Životić I. Estradiol Protects Ovariectomized Female Rats Against Fructose-Rich Diet Induced Oxidative Stress. in Atherosclerosis. 2017;263:E192-E192.
doi:10.1016/j.atherosclerosis.2017.06.616 .

Bošković, Maja, Bundalo, Maja M., Stojiljković, Mojca D., Kostić, Milan, Živković, Maja, Korićanac, Goran, Stanković, Aleksandra, Životić, Ivan, "Estradiol Protects Ovariectomized Female Rats Against Fructose-Rich Diet Induced Oxidative Stress" in Atherosclerosis, 263 (2017):E192-E192,
https://doi.org/10.1016/j.atherosclerosis.2017.06.616 . .

TY  - JOUR
AU  - Životić, Ivan
AU  - Đurić, Tamara
AU  - Stanković, Aleksandra
AU  - Đorđević, Ana
AU  - Končar, Igor
AU  - Davidović, Lazar
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1126
AB  - Single nucleotide polymorphisms from the chromosome locus 9p21 are reported to carry a risk for various cardiovascular diseases. One of the lead single nucleotide polymorphisms, rs10757278, was mostly investigated in association with coronary artery disease but rarely with carotid atherosclerosis. In this study, we aimed to analyze the association of rs10757278 A/G polymorphism with carotid plaque presence in advanced carotid atherosclerosis. The study included 803 participants, 486 patients with high-grade stenosis ( GT 70%) who were undergoing carotid endarterectomy and 317 controls from Serbian population. Genotypes were determined using the real-time polymerase chain reaction. According to the recessive model of inheritance, GG genotype was significantly and independently associated with carotid plaque in females only (odds ratio 2.42, CI = 1.20-4.90, P = 0.013). Odds ratio was adjusted for age, body mass index, hypertension, TC, LDLC, HDLC and TG, and P value was corrected for multiple comparisons. Our preliminary findings suggest a gender-specific association of rs10757278 polymorphism with carotid plaque. Further studies on larger sample and in genetically and environmentally similar populations are needed.
T2  - Experimental Biology and Medicine
T1  - 9p21 locus rs10757278 is associated with advanced carotid atherosclerosis in a gender-specific manner
VL  - 241
IS  - 11
SP  - 1210
EP  - 1216
DO  - 10.1177/1535370216636718
ER  - 

@article{
author = "Životić, Ivan and Đurić, Tamara and Stanković, Aleksandra and Đorđević, Ana and Končar, Igor and Davidović, Lazar and Alavantić, Dragan and Živković, Maja",
year = "2016",
abstract = "Single nucleotide polymorphisms from the chromosome locus 9p21 are reported to carry a risk for various cardiovascular diseases. One of the lead single nucleotide polymorphisms, rs10757278, was mostly investigated in association with coronary artery disease but rarely with carotid atherosclerosis. In this study, we aimed to analyze the association of rs10757278 A/G polymorphism with carotid plaque presence in advanced carotid atherosclerosis. The study included 803 participants, 486 patients with high-grade stenosis ( GT 70%) who were undergoing carotid endarterectomy and 317 controls from Serbian population. Genotypes were determined using the real-time polymerase chain reaction. According to the recessive model of inheritance, GG genotype was significantly and independently associated with carotid plaque in females only (odds ratio 2.42, CI = 1.20-4.90, P = 0.013). Odds ratio was adjusted for age, body mass index, hypertension, TC, LDLC, HDLC and TG, and P value was corrected for multiple comparisons. Our preliminary findings suggest a gender-specific association of rs10757278 polymorphism with carotid plaque. Further studies on larger sample and in genetically and environmentally similar populations are needed.",
journal = "Experimental Biology and Medicine",
title = "9p21 locus rs10757278 is associated with advanced carotid atherosclerosis in a gender-specific manner",
volume = "241",
number = "11",
pages = "1210-1216",
doi = "10.1177/1535370216636718"
}

Životić, I., Đurić, T., Stanković, A., Đorđević, A., Končar, I., Davidović, L., Alavantić, D.,& Živković, M.. (2016). 9p21 locus rs10757278 is associated with advanced carotid atherosclerosis in a gender-specific manner. in Experimental Biology and Medicine, 241(11), 1210-1216.
https://doi.org/10.1177/1535370216636718

Životić I, Đurić T, Stanković A, Đorđević A, Končar I, Davidović L, Alavantić D, Živković M. 9p21 locus rs10757278 is associated with advanced carotid atherosclerosis in a gender-specific manner. in Experimental Biology and Medicine. 2016;241(11):1210-1216.
doi:10.1177/1535370216636718 .

Životić, Ivan, Đurić, Tamara, Stanković, Aleksandra, Đorđević, Ana, Končar, Igor, Davidović, Lazar, Alavantić, Dragan, Živković, Maja, "9p21 locus rs10757278 is associated with advanced carotid atherosclerosis in a gender-specific manner" in Experimental Biology and Medicine, 241, no. 11 (2016):1210-1216,
https://doi.org/10.1177/1535370216636718 . .

TY  - JOUR
AU  - Đorđević, Ana
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Životić, Ivan
AU  - Končar, Igor
AU  - Davidović, Lazar
AU  - Alavantić, Dragan
AU  - Đurić, Tamara
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1291
AB  - BackgroundPrevious research has shown that there is an association between galectin-3 (gal-3) protein and cardiovascular pathology. The aim of this study was to investigate the effects of rs2274273 and rs17128183 on genetic susceptibility to advanced carotid atherosclerosis (CA) and its complications. The rs2274273 has been singled out as the lead SNP of the haplotype block containing LGALS-3 (gal-3 gene) associated with gal-3 circulating levels, while rs17128183 constitutes a potentially functional SNP of the same hap-block. We further sought to determine whether these genetic variants have an impact on the expression of LGALS-3 mRNA in human carotid atherosclerotic plaque tissue. MethodsThe study encompassed 300 control subjects and 485 patients with advanced CA who had undergone carotid endarterectomy. Rs2274273, rs17128183, and LGALS-3 relative mRNA expression was detected by means of real-time PCR (TaqMan((R)) technology). ResultsThere were no statistically significant associations of the investigated genetic variants with susceptibility to advanced CA, nor did we find any associations in terms of ultrasonographically defined plaque phenotypes. The relative expression of LGALS-3 mRNA proved to be significantly higher in carriers of the rare alleles (P=0.039) for both genetic variants. ConclusionOur exploratory results suggest that while rs2274273 and rs17128183 bear no association with the risk of advanced CA or CA-related complications, these genetic variants are likely to affect LGALS-3 expression levels. In order to reach a definitive conclusion on the role played by rs2274273 and rs17128183 in advanced CA, our results should be further validated.
T2  - Journal of Clinical Laboratory Analysis
T1  - Genetic Variants in the Vicinity of LGALS-3 Gene and LGALS-3 mRNA Expression in Advanced Carotid Atherosclerosis: An Exploratory Study
VL  - 30
IS  - 6
SP  - 1150
EP  - 1157
DO  - 10.1002/jcla.21996
ER  - 

@article{
author = "Đorđević, Ana and Živković, Maja and Stanković, Aleksandra and Životić, Ivan and Končar, Igor and Davidović, Lazar and Alavantić, Dragan and Đurić, Tamara",
year = "2016",
abstract = "BackgroundPrevious research has shown that there is an association between galectin-3 (gal-3) protein and cardiovascular pathology. The aim of this study was to investigate the effects of rs2274273 and rs17128183 on genetic susceptibility to advanced carotid atherosclerosis (CA) and its complications. The rs2274273 has been singled out as the lead SNP of the haplotype block containing LGALS-3 (gal-3 gene) associated with gal-3 circulating levels, while rs17128183 constitutes a potentially functional SNP of the same hap-block. We further sought to determine whether these genetic variants have an impact on the expression of LGALS-3 mRNA in human carotid atherosclerotic plaque tissue. MethodsThe study encompassed 300 control subjects and 485 patients with advanced CA who had undergone carotid endarterectomy. Rs2274273, rs17128183, and LGALS-3 relative mRNA expression was detected by means of real-time PCR (TaqMan((R)) technology). ResultsThere were no statistically significant associations of the investigated genetic variants with susceptibility to advanced CA, nor did we find any associations in terms of ultrasonographically defined plaque phenotypes. The relative expression of LGALS-3 mRNA proved to be significantly higher in carriers of the rare alleles (P=0.039) for both genetic variants. ConclusionOur exploratory results suggest that while rs2274273 and rs17128183 bear no association with the risk of advanced CA or CA-related complications, these genetic variants are likely to affect LGALS-3 expression levels. In order to reach a definitive conclusion on the role played by rs2274273 and rs17128183 in advanced CA, our results should be further validated.",
journal = "Journal of Clinical Laboratory Analysis",
title = "Genetic Variants in the Vicinity of LGALS-3 Gene and LGALS-3 mRNA Expression in Advanced Carotid Atherosclerosis: An Exploratory Study",
volume = "30",
number = "6",
pages = "1150-1157",
doi = "10.1002/jcla.21996"
}

Đorđević, A., Živković, M., Stanković, A., Životić, I., Končar, I., Davidović, L., Alavantić, D.,& Đurić, T.. (2016). Genetic Variants in the Vicinity of LGALS-3 Gene and LGALS-3 mRNA Expression in Advanced Carotid Atherosclerosis: An Exploratory Study. in Journal of Clinical Laboratory Analysis, 30(6), 1150-1157.
https://doi.org/10.1002/jcla.21996

Đorđević A, Živković M, Stanković A, Životić I, Končar I, Davidović L, Alavantić D, Đurić T. Genetic Variants in the Vicinity of LGALS-3 Gene and LGALS-3 mRNA Expression in Advanced Carotid Atherosclerosis: An Exploratory Study. in Journal of Clinical Laboratory Analysis. 2016;30(6):1150-1157.
doi:10.1002/jcla.21996 .

Đorđević, Ana, Živković, Maja, Stanković, Aleksandra, Životić, Ivan, Končar, Igor, Davidović, Lazar, Alavantić, Dragan, Đurić, Tamara, "Genetic Variants in the Vicinity of LGALS-3 Gene and LGALS-3 mRNA Expression in Advanced Carotid Atherosclerosis: An Exploratory Study" in Journal of Clinical Laboratory Analysis, 30, no. 6 (2016):1150-1157,
https://doi.org/10.1002/jcla.21996 . .

TY  - CONF
AU  - Đorđević, Ana
AU  - Đurić, Tamara
AU  - Živković, Maja
AU  - Životić, Ivan
AU  - Dekleva, Milica
AU  - Marković-Nikolić, Nataša
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1101
C3  - European Journal of Heart Failure
T1  - Rs2274273 from LGALS-3 locus and cardiac ventricular remodeling after first myocardial infarction: preliminary results
VL  - 18
IS  - SI
SP  - 283
EP  - 284
UR  - https://hdl.handle.net/21.15107/rcub_vinar_1101
ER  - 

@conference{
author = "Đorđević, Ana and Đurić, Tamara and Živković, Maja and Životić, Ivan and Dekleva, Milica and Marković-Nikolić, Nataša and Alavantić, Dragan and Stanković, Aleksandra",
year = "2016",
journal = "European Journal of Heart Failure",
title = "Rs2274273 from LGALS-3 locus and cardiac ventricular remodeling after first myocardial infarction: preliminary results",
volume = "18",
number = "SI",
pages = "283-284",
url = "https://hdl.handle.net/21.15107/rcub_vinar_1101"
}

Đorđević, A., Đurić, T., Živković, M., Životić, I., Dekleva, M., Marković-Nikolić, N., Alavantić, D.,& Stanković, A.. (2016). Rs2274273 from LGALS-3 locus and cardiac ventricular remodeling after first myocardial infarction: preliminary results. in European Journal of Heart Failure, 18(SI), 283-284.
https://hdl.handle.net/21.15107/rcub_vinar_1101

Đorđević A, Đurić T, Živković M, Životić I, Dekleva M, Marković-Nikolić N, Alavantić D, Stanković A. Rs2274273 from LGALS-3 locus and cardiac ventricular remodeling after first myocardial infarction: preliminary results. in European Journal of Heart Failure. 2016;18(SI):283-284.
https://hdl.handle.net/21.15107/rcub_vinar_1101 .

Đorđević, Ana, Đurić, Tamara, Živković, Maja, Životić, Ivan, Dekleva, Milica, Marković-Nikolić, Nataša, Alavantić, Dragan, Stanković, Aleksandra, "Rs2274273 from LGALS-3 locus and cardiac ventricular remodeling after first myocardial infarction: preliminary results" in European Journal of Heart Failure, 18, no. SI (2016):283-284,
https://hdl.handle.net/21.15107/rcub_vinar_1101 .

TY  - CONF
AU  - Životić, Ivan
AU  - Đurić-Delić, Tamara
AU  - Živković, Maja
AU  - Bojić, Tijana
AU  - Milovanović, Branislav
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11401
PB  - Belgrade : Serbian Neurocardiological Society
C3  - NEUROCARD 2015 : 7th Congress of Serbian neuroscience society with international participation, 6th International Symposium on Noninvasive Electrocardiology :  Program and the book of abstracts
T1  - The eNOS rs1799983 gene polymorphism (Glu298Asp) in association with cardiovascular profiles in patients with vasovagal syncope
SP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11401
ER  - 

@conference{
author = "Životić, Ivan and Đurić-Delić, Tamara and Živković, Maja and Bojić, Tijana and Milovanović, Branislav and Alavantić, Dragan and Stanković, Aleksandra",
year = "2015",
publisher = "Belgrade : Serbian Neurocardiological Society",
journal = "NEUROCARD 2015 : 7th Congress of Serbian neuroscience society with international participation, 6th International Symposium on Noninvasive Electrocardiology :  Program and the book of abstracts",
title = "The eNOS rs1799983 gene polymorphism (Glu298Asp) in association with cardiovascular profiles in patients with vasovagal syncope",
pages = "33",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11401"
}

Životić, I., Đurić-Delić, T., Živković, M., Bojić, T., Milovanović, B., Alavantić, D.,& Stanković, A.. (2015). The eNOS rs1799983 gene polymorphism (Glu298Asp) in association with cardiovascular profiles in patients with vasovagal syncope. in NEUROCARD 2015 : 7th Congress of Serbian neuroscience society with international participation, 6th International Symposium on Noninvasive Electrocardiology :  Program and the book of abstracts
Belgrade : Serbian Neurocardiological Society., 33.
https://hdl.handle.net/21.15107/rcub_vinar_11401

Životić I, Đurić-Delić T, Živković M, Bojić T, Milovanović B, Alavantić D, Stanković A. The eNOS rs1799983 gene polymorphism (Glu298Asp) in association with cardiovascular profiles in patients with vasovagal syncope. in NEUROCARD 2015 : 7th Congress of Serbian neuroscience society with international participation, 6th International Symposium on Noninvasive Electrocardiology :  Program and the book of abstracts. 2015;:33.
https://hdl.handle.net/21.15107/rcub_vinar_11401 .

Životić, Ivan, Đurić-Delić, Tamara, Živković, Maja, Bojić, Tijana, Milovanović, Branislav, Alavantić, Dragan, Stanković, Aleksandra, "The eNOS rs1799983 gene polymorphism (Glu298Asp) in association with cardiovascular profiles in patients with vasovagal syncope" in NEUROCARD 2015 : 7th Congress of Serbian neuroscience society with international participation, 6th International Symposium on Noninvasive Electrocardiology :  Program and the book of abstracts (2015):33,
https://hdl.handle.net/21.15107/rcub_vinar_11401 .