TY  - JOUR
AU  - Van Gool, Alain
AU  - Corrales, Fernado
AU  - Čolović, Mirjana B.
AU  - Krstić, Danijela Z.
AU  - Oliver-Martos, Begona
AU  - Martínez-Cáceres, Eva
AU  - Jakasa, Ivone
AU  - Gajski, Goran
AU  - Brun, Virginie
AU  - Kyriacou, Kyriacos
AU  - Burzynska-Pedziwiatr, Izabela
AU  - Wozniak, Lucyna Alicja
AU  - Nierkens, Stephan
AU  - Pascual García, César
AU  - Katrlik, Jaroslav
AU  - Bojić-Trbojević, Žanka
AU  - Vacek, Jan
AU  - Llorente, Alicia
AU  - Antohe, Felicia
AU  - Suica, Viorel
AU  - Suarez, Guillaume
AU  - T’Kindt, Ruben
AU  - Martin, Petra
AU  - Penque, Deborah
AU  - Martins, Ines Lanca
AU  - Bodoki, Ede
AU  - Iacob, Bogdan-Cezar
AU  - Aydindogan, Eda
AU  - Timur, Suna
AU  - Allinson, John
AU  - Sutton, Christopher
AU  - Luider, Theo
AU  - Wittfooth, Saara
AU  - Sammar, Marei
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9018
AB  - Introduction: The importance of biomarkers for pharmaceutical drug development and clinical diagnostics is more significant than ever in the current shift toward personalized medicine. Biomarkers have taken a central position either as companion markers to support drug development and patient selection, or as indicators aiming to detect the earliest perturbations indicative of disease, minimizing therapeutic intervention or even enabling disease reversal. Protein biomarkers are of particular interest given their central role in biochemical pathways. Hence, capabilities to analyze multiple protein biomarkers in one assay are highly interesting for biomedical research. Areas covered: We here review multiple methods that are suitable for robust, high throughput, standardized, and affordable analysis of protein biomarkers in a multiplex format. We describe innovative developments in immunoassays, the vanguard of methods in clinical laboratories, and mass spectrometry, increasingly implemented for protein biomarker analysis. Moreover, emerging techniques are discussed with potentially improved protein capture, separation, and detection that will further boost multiplex analyses. Expert commentary: The development of clinically applied multiplex protein biomarker assays is essential as multi-protein signatures provide more comprehensive information about biological systems than single biomarkers, leading to improved insights in mechanisms of disease, diagnostics, and the effect of personalized medicine.
T2  - Expert Review of Proteomics
T1  - Analytical techniques for multiplex analysis of protein biomarkers
VL  - 17
IS  - 4
SP  - 257
EP  - 273
DO  - 10.1080/14789450.2020.1763174
ER  - 

@article{
author = "Van Gool, Alain and Corrales, Fernado and Čolović, Mirjana B. and Krstić, Danijela Z. and Oliver-Martos, Begona and Martínez-Cáceres, Eva and Jakasa, Ivone and Gajski, Goran and Brun, Virginie and Kyriacou, Kyriacos and Burzynska-Pedziwiatr, Izabela and Wozniak, Lucyna Alicja and Nierkens, Stephan and Pascual García, César and Katrlik, Jaroslav and Bojić-Trbojević, Žanka and Vacek, Jan and Llorente, Alicia and Antohe, Felicia and Suica, Viorel and Suarez, Guillaume and T’Kindt, Ruben and Martin, Petra and Penque, Deborah and Martins, Ines Lanca and Bodoki, Ede and Iacob, Bogdan-Cezar and Aydindogan, Eda and Timur, Suna and Allinson, John and Sutton, Christopher and Luider, Theo and Wittfooth, Saara and Sammar, Marei",
year = "2020",
abstract = "Introduction: The importance of biomarkers for pharmaceutical drug development and clinical diagnostics is more significant than ever in the current shift toward personalized medicine. Biomarkers have taken a central position either as companion markers to support drug development and patient selection, or as indicators aiming to detect the earliest perturbations indicative of disease, minimizing therapeutic intervention or even enabling disease reversal. Protein biomarkers are of particular interest given their central role in biochemical pathways. Hence, capabilities to analyze multiple protein biomarkers in one assay are highly interesting for biomedical research. Areas covered: We here review multiple methods that are suitable for robust, high throughput, standardized, and affordable analysis of protein biomarkers in a multiplex format. We describe innovative developments in immunoassays, the vanguard of methods in clinical laboratories, and mass spectrometry, increasingly implemented for protein biomarker analysis. Moreover, emerging techniques are discussed with potentially improved protein capture, separation, and detection that will further boost multiplex analyses. Expert commentary: The development of clinically applied multiplex protein biomarker assays is essential as multi-protein signatures provide more comprehensive information about biological systems than single biomarkers, leading to improved insights in mechanisms of disease, diagnostics, and the effect of personalized medicine.",
journal = "Expert Review of Proteomics",
title = "Analytical techniques for multiplex analysis of protein biomarkers",
volume = "17",
number = "4",
pages = "257-273",
doi = "10.1080/14789450.2020.1763174"
}

Van Gool, A., Corrales, F., Čolović, M. B., Krstić, D. Z., Oliver-Martos, B., Martínez-Cáceres, E., Jakasa, I., Gajski, G., Brun, V., Kyriacou, K., Burzynska-Pedziwiatr, I., Wozniak, L. A., Nierkens, S., Pascual García, C., Katrlik, J., Bojić-Trbojević, Ž., Vacek, J., Llorente, A., Antohe, F., Suica, V., Suarez, G., T’Kindt, R., Martin, P., Penque, D., Martins, I. L., Bodoki, E., Iacob, B., Aydindogan, E., Timur, S., Allinson, J., Sutton, C., Luider, T., Wittfooth, S.,& Sammar, M.. (2020). Analytical techniques for multiplex analysis of protein biomarkers. in Expert Review of Proteomics, 17(4), 257-273.
https://doi.org/10.1080/14789450.2020.1763174

Van Gool A, Corrales F, Čolović MB, Krstić DZ, Oliver-Martos B, Martínez-Cáceres E, Jakasa I, Gajski G, Brun V, Kyriacou K, Burzynska-Pedziwiatr I, Wozniak LA, Nierkens S, Pascual García C, Katrlik J, Bojić-Trbojević Ž, Vacek J, Llorente A, Antohe F, Suica V, Suarez G, T’Kindt R, Martin P, Penque D, Martins IL, Bodoki E, Iacob B, Aydindogan E, Timur S, Allinson J, Sutton C, Luider T, Wittfooth S, Sammar M. Analytical techniques for multiplex analysis of protein biomarkers. in Expert Review of Proteomics. 2020;17(4):257-273.
doi:10.1080/14789450.2020.1763174 .

Van Gool, Alain, Corrales, Fernado, Čolović, Mirjana B., Krstić, Danijela Z., Oliver-Martos, Begona, Martínez-Cáceres, Eva, Jakasa, Ivone, Gajski, Goran, Brun, Virginie, Kyriacou, Kyriacos, Burzynska-Pedziwiatr, Izabela, Wozniak, Lucyna Alicja, Nierkens, Stephan, Pascual García, César, Katrlik, Jaroslav, Bojić-Trbojević, Žanka, Vacek, Jan, Llorente, Alicia, Antohe, Felicia, Suica, Viorel, Suarez, Guillaume, T’Kindt, Ruben, Martin, Petra, Penque, Deborah, Martins, Ines Lanca, Bodoki, Ede, Iacob, Bogdan-Cezar, Aydindogan, Eda, Timur, Suna, Allinson, John, Sutton, Christopher, Luider, Theo, Wittfooth, Saara, Sammar, Marei, "Analytical techniques for multiplex analysis of protein biomarkers" in Expert Review of Proteomics, 17, no. 4 (2020):257-273,
https://doi.org/10.1080/14789450.2020.1763174 . .