TY  - JOUR
AU  - Gluvić, Zoran
AU  - Obradović, Milan M.
AU  - Stewart, Alan J.
AU  - Essack, Magbubah
AU  - Pitt, Samantha J.
AU  - Samardžić, Vladimir
AU  - Soskić, Sanja S.
AU  - Gojobori, Takashi
AU  - Isenović, Esma R.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10047
AB  - Levothyroxine (LT4) is used to treat frequently encountered endocrinopathies such as thyroid diseases. It is regularly used in clinical (overt) hypothyroidism cases and subclinical (latent) hypothyroidism cases in the last decade. Suppressive LT4 therapy is also part of the medical regimen used to manage thyroid malignancies after a thyroidectomy. LT4 treatment possesses dual effects: substituting new-onset thyroid hormone deficiency and suppressing the local and distant malignancy spreading in cancer. It is the practice to administer LT4 in less-than-high suppressive doses for growth control of thyroid nodules and goiter, even in patients with preserved thyroid function. Despite its approved safety for clinical use, LT4 can sometimes induce side-effects, more often recorded with patients under treatment with LT4 suppressive doses than in unintentionally LT4-overdosed patients. Cardiac arrhythmias and the deterioration of osteoporosis are the most frequently documented side-effects of LT4 therapy. It also lowers the threshold for the onset or aggravation of cardiac arrhythmias for patients with pre-existing heart diseases. To improve the quality of life in LT4-substituted patients, clinicians often prescribe higher doses of LT4 to reach low normal TSH levels to achieve cellular euthyroidism. In such circumstances, the risk of cardiac arrhythmias, particularly atrial fibrillation, increases, and the combined use of LT4 and triiodothyronine further complicates such risk. This review summarizes the relevant available data related to LT4 suppressive treatment and the associated risk of cardiac arrhythmia.
T2  - Frontiers in Endocrinology
T1  - Levothyroxine Treatment and the Risk of Cardiac Arrhythmias – Focus on the Patient Submitted to Thyroid Surgery
VL  - 12
SP  - 1415
DO  - 10.3389/fendo.2021.758043
ER  - 

@article{
author = "Gluvić, Zoran and Obradović, Milan M. and Stewart, Alan J. and Essack, Magbubah and Pitt, Samantha J. and Samardžić, Vladimir and Soskić, Sanja S. and Gojobori, Takashi and Isenović, Esma R.",
year = "2021",
abstract = "Levothyroxine (LT4) is used to treat frequently encountered endocrinopathies such as thyroid diseases. It is regularly used in clinical (overt) hypothyroidism cases and subclinical (latent) hypothyroidism cases in the last decade. Suppressive LT4 therapy is also part of the medical regimen used to manage thyroid malignancies after a thyroidectomy. LT4 treatment possesses dual effects: substituting new-onset thyroid hormone deficiency and suppressing the local and distant malignancy spreading in cancer. It is the practice to administer LT4 in less-than-high suppressive doses for growth control of thyroid nodules and goiter, even in patients with preserved thyroid function. Despite its approved safety for clinical use, LT4 can sometimes induce side-effects, more often recorded with patients under treatment with LT4 suppressive doses than in unintentionally LT4-overdosed patients. Cardiac arrhythmias and the deterioration of osteoporosis are the most frequently documented side-effects of LT4 therapy. It also lowers the threshold for the onset or aggravation of cardiac arrhythmias for patients with pre-existing heart diseases. To improve the quality of life in LT4-substituted patients, clinicians often prescribe higher doses of LT4 to reach low normal TSH levels to achieve cellular euthyroidism. In such circumstances, the risk of cardiac arrhythmias, particularly atrial fibrillation, increases, and the combined use of LT4 and triiodothyronine further complicates such risk. This review summarizes the relevant available data related to LT4 suppressive treatment and the associated risk of cardiac arrhythmia.",
journal = "Frontiers in Endocrinology",
title = "Levothyroxine Treatment and the Risk of Cardiac Arrhythmias – Focus on the Patient Submitted to Thyroid Surgery",
volume = "12",
pages = "1415",
doi = "10.3389/fendo.2021.758043"
}

Gluvić, Z., Obradović, M. M., Stewart, A. J., Essack, M., Pitt, S. J., Samardžić, V., Soskić, S. S., Gojobori, T.,& Isenović, E. R.. (2021). Levothyroxine Treatment and the Risk of Cardiac Arrhythmias – Focus on the Patient Submitted to Thyroid Surgery. in Frontiers in Endocrinology, 12, 1415.
https://doi.org/10.3389/fendo.2021.758043

Gluvić Z, Obradović MM, Stewart AJ, Essack M, Pitt SJ, Samardžić V, Soskić SS, Gojobori T, Isenović ER. Levothyroxine Treatment and the Risk of Cardiac Arrhythmias – Focus on the Patient Submitted to Thyroid Surgery. in Frontiers in Endocrinology. 2021;12:1415.
doi:10.3389/fendo.2021.758043 .

Gluvić, Zoran, Obradović, Milan M., Stewart, Alan J., Essack, Magbubah, Pitt, Samantha J., Samardžić, Vladimir, Soskić, Sanja S., Gojobori, Takashi, Isenović, Esma R., "Levothyroxine Treatment and the Risk of Cardiac Arrhythmias – Focus on the Patient Submitted to Thyroid Surgery" in Frontiers in Endocrinology, 12 (2021):1415,
https://doi.org/10.3389/fendo.2021.758043 . .

TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Sudar, Emina
AU  - Obradović, Milan M.
AU  - Pitt, Samantha J.
AU  - Stewart, Alan J.
AU  - Zafirović, Sonja
AU  - Stanimirović, Julijana
AU  - Radak, Đorđe J.
AU  - Isenović, Esma R.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1678
AB  - Background: Overexpression of inducible nitric oxide synthase (iNOS) is a key link between high-fat (HF) diet induced obesity and cardiovascular disease. Oestradiol has cardioprotective effects that may be mediated through reduction of iNOS activity/expression. Methods: In the present study, female Wistar rats were fed a standard diet or a HF diet (42% fat) for 10 weeks. iNOS gene and protein expressions were measured in heart tissue. HF-fed rats exhibited a significant increase in cardiac iNOS mRNA by 695% (p LT 0.05), iNOS protein level by 248% (p LT 0.01), without changes in nitrate/nitrite levels. Expression of CD36 protein in plasma membranes was increased by 37% (p LT 0.05), while the concentration of free fatty acids (FFA) was reduced by 25% (p LT 0.01) in HF-fed rats. Expression of the p50 subunit of nuclear factor-kappa B (NF kappa B-p50) in heart was increased by 77% (p LT 0.01) in HF-fed rats. Expression of protein kinase B (Akt) and extracellular signal-regulated kinases 1/2 (ERK1/2) were unchanged between the groups. There was a significant increase in the ratio of phospho-Akt/total Akt but not for phospho-ERK1/2/total ERK1/2 in HF-fed rats. Estrogen receptor-levels (by 50%; p LT 0.05) and serum oestradiol concentrations (by 35%; p LT 0.05) were shown to be significantly reduced in HF-fed rats. Results and Conclusion: Our results revealed that a HF diet led to increased iNOS expression, most likely via a mechanism involving Akt and NF kappa B-p50 proteins. Decreased levels of oestradiol and ER protein in the HF-fed group, in combination with increased iNOS levels are consistent with the hypothesis that oestradiol has a cardioprotective effect through its ability to regulate iNOS expression.
T2  - Current Vascular Pharmacology
T1  - Influence of a High-fat Diet on Cardiac iNOS in Female Rats
VL  - 15
IS  - 5
SP  - 491
EP  - 500
DO  - 10.2174/1570161114666161025101303
ER  - 

@article{
author = "Jovanović, Aleksandra and Sudar, Emina and Obradović, Milan M. and Pitt, Samantha J. and Stewart, Alan J. and Zafirović, Sonja and Stanimirović, Julijana and Radak, Đorđe J. and Isenović, Esma R.",
year = "2017",
abstract = "Background: Overexpression of inducible nitric oxide synthase (iNOS) is a key link between high-fat (HF) diet induced obesity and cardiovascular disease. Oestradiol has cardioprotective effects that may be mediated through reduction of iNOS activity/expression. Methods: In the present study, female Wistar rats were fed a standard diet or a HF diet (42% fat) for 10 weeks. iNOS gene and protein expressions were measured in heart tissue. HF-fed rats exhibited a significant increase in cardiac iNOS mRNA by 695% (p LT 0.05), iNOS protein level by 248% (p LT 0.01), without changes in nitrate/nitrite levels. Expression of CD36 protein in plasma membranes was increased by 37% (p LT 0.05), while the concentration of free fatty acids (FFA) was reduced by 25% (p LT 0.01) in HF-fed rats. Expression of the p50 subunit of nuclear factor-kappa B (NF kappa B-p50) in heart was increased by 77% (p LT 0.01) in HF-fed rats. Expression of protein kinase B (Akt) and extracellular signal-regulated kinases 1/2 (ERK1/2) were unchanged between the groups. There was a significant increase in the ratio of phospho-Akt/total Akt but not for phospho-ERK1/2/total ERK1/2 in HF-fed rats. Estrogen receptor-levels (by 50%; p LT 0.05) and serum oestradiol concentrations (by 35%; p LT 0.05) were shown to be significantly reduced in HF-fed rats. Results and Conclusion: Our results revealed that a HF diet led to increased iNOS expression, most likely via a mechanism involving Akt and NF kappa B-p50 proteins. Decreased levels of oestradiol and ER protein in the HF-fed group, in combination with increased iNOS levels are consistent with the hypothesis that oestradiol has a cardioprotective effect through its ability to regulate iNOS expression.",
journal = "Current Vascular Pharmacology",
title = "Influence of a High-fat Diet on Cardiac iNOS in Female Rats",
volume = "15",
number = "5",
pages = "491-500",
doi = "10.2174/1570161114666161025101303"
}

Jovanović, A., Sudar, E., Obradović, M. M., Pitt, S. J., Stewart, A. J., Zafirović, S., Stanimirović, J., Radak, Đ. J.,& Isenović, E. R.. (2017). Influence of a High-fat Diet on Cardiac iNOS in Female Rats. in Current Vascular Pharmacology, 15(5), 491-500.
https://doi.org/10.2174/1570161114666161025101303

Jovanović A, Sudar E, Obradović MM, Pitt SJ, Stewart AJ, Zafirović S, Stanimirović J, Radak ĐJ, Isenović ER. Influence of a High-fat Diet on Cardiac iNOS in Female Rats. in Current Vascular Pharmacology. 2017;15(5):491-500.
doi:10.2174/1570161114666161025101303 .

Jovanović, Aleksandra, Sudar, Emina, Obradović, Milan M., Pitt, Samantha J., Stewart, Alan J., Zafirović, Sonja, Stanimirović, Julijana, Radak, Đorđe J., Isenović, Esma R., "Influence of a High-fat Diet on Cardiac iNOS in Female Rats" in Current Vascular Pharmacology, 15, no. 5 (2017):491-500,
https://doi.org/10.2174/1570161114666161025101303 . .

TY  - JOUR
AU  - Zafirović, Sonja
AU  - Obradović, Milan M.
AU  - Sudar-Milovanović, Emina
AU  - Jovanović, Aleksandra
AU  - Stanimirović, Julijana
AU  - Stewart, Alan J.
AU  - Pitt, Samantha J.
AU  - Isenović, Esma R.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1520
AB  - The aim of this study was to investigate the in vivo effects of 17 beta-estradiol (E-2) on myocardial metabolism and inducible nitric oxide synthase (iNOS) expression/activity in obese rats. Male Wistar rats were fed with a normal or a high fat (HF) diet (42% fat) for 10 weeks. Half of the HF fed rats were treated with a single dose of E-2 while the other half were placebo-treated. 24 h after treatment animals were sacrificed. E-2 reduced cardiac free fatty acid (FFA) (p LT 0.05), L-arginine (p LT 0.01), iNOS mRNA (p LT 0.01), and protein (p LT 0.05) levels and translocation of the FFA transporter (CD36) (p LT 0.01) to the plasma membrane (PM) in HF fed rats. In contrast, Akt phosphorylation at Thr308 (p LT 0.05) and translocation of the glucose transporter GLUT4 (p LT 0.05) to the PM increased after E-2 treatment in HF rats. Our results indicate that E-2 acts via the PI3K/Akt signalling pathway to partially protect myocardial metabolism by attenuating the detrimental effects of increased iNOS expression/activity in HF fed rats. (C) 2017 Elsevier B.V. All rights reserved.
T2  - Molecular and Cellular Endocrinology
T1  - 17 beta-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats
VL  - 446
IS  - C
SP  - 12
EP  - 20
DO  - 10.1016/j.mce.2017.02.001
ER  - 

@article{
author = "Zafirović, Sonja and Obradović, Milan M. and Sudar-Milovanović, Emina and Jovanović, Aleksandra and Stanimirović, Julijana and Stewart, Alan J. and Pitt, Samantha J. and Isenović, Esma R.",
year = "2017",
abstract = "The aim of this study was to investigate the in vivo effects of 17 beta-estradiol (E-2) on myocardial metabolism and inducible nitric oxide synthase (iNOS) expression/activity in obese rats. Male Wistar rats were fed with a normal or a high fat (HF) diet (42% fat) for 10 weeks. Half of the HF fed rats were treated with a single dose of E-2 while the other half were placebo-treated. 24 h after treatment animals were sacrificed. E-2 reduced cardiac free fatty acid (FFA) (p LT 0.05), L-arginine (p LT 0.01), iNOS mRNA (p LT 0.01), and protein (p LT 0.05) levels and translocation of the FFA transporter (CD36) (p LT 0.01) to the plasma membrane (PM) in HF fed rats. In contrast, Akt phosphorylation at Thr308 (p LT 0.05) and translocation of the glucose transporter GLUT4 (p LT 0.05) to the PM increased after E-2 treatment in HF rats. Our results indicate that E-2 acts via the PI3K/Akt signalling pathway to partially protect myocardial metabolism by attenuating the detrimental effects of increased iNOS expression/activity in HF fed rats. (C) 2017 Elsevier B.V. All rights reserved.",
journal = "Molecular and Cellular Endocrinology",
title = "17 beta-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats",
volume = "446",
number = "C",
pages = "12-20",
doi = "10.1016/j.mce.2017.02.001"
}

Zafirović, S., Obradović, M. M., Sudar-Milovanović, E., Jovanović, A., Stanimirović, J., Stewart, A. J., Pitt, S. J.,& Isenović, E. R.. (2017). 17 beta-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats. in Molecular and Cellular Endocrinology, 446(C), 12-20.
https://doi.org/10.1016/j.mce.2017.02.001

Zafirović S, Obradović MM, Sudar-Milovanović E, Jovanović A, Stanimirović J, Stewart AJ, Pitt SJ, Isenović ER. 17 beta-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats. in Molecular and Cellular Endocrinology. 2017;446(C):12-20.
doi:10.1016/j.mce.2017.02.001 .

Zafirović, Sonja, Obradović, Milan M., Sudar-Milovanović, Emina, Jovanović, Aleksandra, Stanimirović, Julijana, Stewart, Alan J., Pitt, Samantha J., Isenović, Esma R., "17 beta-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats" in Molecular and Cellular Endocrinology, 446, no. C (2017):12-20,
https://doi.org/10.1016/j.mce.2017.02.001 . .

TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Obradović, Milan M.
AU  - Sudar-Milovanović, Emina
AU  - Stewart, Alan J.
AU  - Pitt, Samantha J.
AU  - Alavantić, Dragan
AU  - Aleksić, Ema
AU  - Isenović, Esma R.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1812
AB  - The aim of this study was to investigate whether the presence of endogenous estradiol alters the effects of a high-fat (HF) diet on activity/expression of the cardiac Na+/K+-ATPase, via PI3K/IRS and RhoA/ROCK signalling cascades in female rats. For this study, female Wistar rats (8 weeks old, 150-200 g) were fed a standard diet or a HF diet (balanced diet for laboratory rats enriched with 42% fat) for 10 weeks. The results show that rats fed a HF diet exhibited a decrease in phosphorylation of the alpha(1) subunit of Na+/K+-ATPase by 30% (p LT 0.05), expression of total alpha(1) subunit of Na+/K+-ATPase by 31% (p LT 0.05), and association of IRS1 with p85 subunit of PI3K by 42% (p LT 0.05), while the levels of cardiac RhoA and ROCK2 were significantly increased by 84% (p LT 0.01) and 62% (p LT 0.05), respectively. Our results suggest that a HF diet alters cardiac Na+/K+-ATPase expression via molecular mechanisms involving RhoA/ROCK and IRS-1/PI3K signalling in female rats.
T2  - Molecular and Cellular Biochemistry
T1  - Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high-fat diet
VL  - 436
IS  - 1-2
SP  - 49
EP  - 58
DO  - 10.1007/s11010-017-3077-y
ER  - 

@article{
author = "Jovanović, Aleksandra and Obradović, Milan M. and Sudar-Milovanović, Emina and Stewart, Alan J. and Pitt, Samantha J. and Alavantić, Dragan and Aleksić, Ema and Isenović, Esma R.",
year = "2017",
abstract = "The aim of this study was to investigate whether the presence of endogenous estradiol alters the effects of a high-fat (HF) diet on activity/expression of the cardiac Na+/K+-ATPase, via PI3K/IRS and RhoA/ROCK signalling cascades in female rats. For this study, female Wistar rats (8 weeks old, 150-200 g) were fed a standard diet or a HF diet (balanced diet for laboratory rats enriched with 42% fat) for 10 weeks. The results show that rats fed a HF diet exhibited a decrease in phosphorylation of the alpha(1) subunit of Na+/K+-ATPase by 30% (p LT 0.05), expression of total alpha(1) subunit of Na+/K+-ATPase by 31% (p LT 0.05), and association of IRS1 with p85 subunit of PI3K by 42% (p LT 0.05), while the levels of cardiac RhoA and ROCK2 were significantly increased by 84% (p LT 0.01) and 62% (p LT 0.05), respectively. Our results suggest that a HF diet alters cardiac Na+/K+-ATPase expression via molecular mechanisms involving RhoA/ROCK and IRS-1/PI3K signalling in female rats.",
journal = "Molecular and Cellular Biochemistry",
title = "Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high-fat diet",
volume = "436",
number = "1-2",
pages = "49-58",
doi = "10.1007/s11010-017-3077-y"
}

Jovanović, A., Obradović, M. M., Sudar-Milovanović, E., Stewart, A. J., Pitt, S. J., Alavantić, D., Aleksić, E.,& Isenović, E. R.. (2017). Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high-fat diet. in Molecular and Cellular Biochemistry, 436(1-2), 49-58.
https://doi.org/10.1007/s11010-017-3077-y

Jovanović A, Obradović MM, Sudar-Milovanović E, Stewart AJ, Pitt SJ, Alavantić D, Aleksić E, Isenović ER. Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high-fat diet. in Molecular and Cellular Biochemistry. 2017;436(1-2):49-58.
doi:10.1007/s11010-017-3077-y .

Jovanović, Aleksandra, Obradović, Milan M., Sudar-Milovanović, Emina, Stewart, Alan J., Pitt, Samantha J., Alavantić, Dragan, Aleksić, Ema, Isenović, Esma R., "Changes in cardiac Na+/K+-ATPase expression and activity in female rats fed a high-fat diet" in Molecular and Cellular Biochemistry, 436, no. 1-2 (2017):49-58,
https://doi.org/10.1007/s11010-017-3077-y . .

TY  - JOUR
AU  - Stanimirović, Julijana
AU  - Obradović, Milan M.
AU  - Jovanović, Aleksandra
AU  - Sudar, Emina
AU  - Zafirović, Sonja
AU  - Pitt, Samantha J.
AU  - Stewart, Alan J.
AU  - Isenović, Esma R.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1004
AB  - Men and women differ substantially with regard to the severity of insulin resistance (IR) but the underlying mechanism(s) of how this occurs is poorly characterized. We investigated whether a high fat (HF) diet resulted in sex-specific differences in nitrite/nitrate production and lipid metabolism and whether these variances may contribute to altered obesity-induced IR. Male and female Wistar rats were fed a standard laboratory diet or a HF diet for 10 weeks. The level of plasma nitrite/nitrate, as well as free fatty acid (FFA), in both plasma and liver lysates were assessed. The levels of inducible nitric oxide (NO) synthase (iNOS), p65 subunit of NF kappa B, total and phosphorylated forms of Akt, mTOR and PDK-1 in lysates, and the levels of glucose transporter 2 (Glut-2) and fatty acid translocase/cluster of differentiation 36 (FAT/CD36) in plasma membrane fractions of liver were assessed. HF-fed male rats exhibited a significant increase in plasma nitrite/nitrate, and hepatic FFA and FAT/CD36 levels compared with controls. They also displayed a relative decrease in iNOS and Glut-2 levels in the liver. Phosphorylation of Akt (at Ser(473) and Thr(308)), mTOR and PDK-1 was also reduced. HF-fed female rats exhibited increased levels of NF kappa B-p65 in liver compared with controls, while levels of Glut-2, FAT/CD36 and Akt phosphorylation at Thr(308) and PDK-1 were decreased. Our results reveal that altered lipid and glucose metabolism in obesity, lead to altered iNOS expression and nitrite/nitrate production. It is likely that this mechanism contributes to sex-specific differences in the development of IR. (C) 2016 Elsevier Inc. All rights reserved.
PB  - Elsevier
T2  - Nitric Oxide: Biology and Chemistry
T1  - A high fat diet induces sex-specific differences in hepatic lipid metabolism and nitrite/nitrate in rats
VL  - 54
SP  - 51
EP  - 59
DO  - 10.1016/j.niox.2016.02.007
ER  - 

@article{
author = "Stanimirović, Julijana and Obradović, Milan M. and Jovanović, Aleksandra and Sudar, Emina and Zafirović, Sonja and Pitt, Samantha J. and Stewart, Alan J. and Isenović, Esma R.",
year = "2016",
abstract = "Men and women differ substantially with regard to the severity of insulin resistance (IR) but the underlying mechanism(s) of how this occurs is poorly characterized. We investigated whether a high fat (HF) diet resulted in sex-specific differences in nitrite/nitrate production and lipid metabolism and whether these variances may contribute to altered obesity-induced IR. Male and female Wistar rats were fed a standard laboratory diet or a HF diet for 10 weeks. The level of plasma nitrite/nitrate, as well as free fatty acid (FFA), in both plasma and liver lysates were assessed. The levels of inducible nitric oxide (NO) synthase (iNOS), p65 subunit of NF kappa B, total and phosphorylated forms of Akt, mTOR and PDK-1 in lysates, and the levels of glucose transporter 2 (Glut-2) and fatty acid translocase/cluster of differentiation 36 (FAT/CD36) in plasma membrane fractions of liver were assessed. HF-fed male rats exhibited a significant increase in plasma nitrite/nitrate, and hepatic FFA and FAT/CD36 levels compared with controls. They also displayed a relative decrease in iNOS and Glut-2 levels in the liver. Phosphorylation of Akt (at Ser(473) and Thr(308)), mTOR and PDK-1 was also reduced. HF-fed female rats exhibited increased levels of NF kappa B-p65 in liver compared with controls, while levels of Glut-2, FAT/CD36 and Akt phosphorylation at Thr(308) and PDK-1 were decreased. Our results reveal that altered lipid and glucose metabolism in obesity, lead to altered iNOS expression and nitrite/nitrate production. It is likely that this mechanism contributes to sex-specific differences in the development of IR. (C) 2016 Elsevier Inc. All rights reserved.",
publisher = "Elsevier",
journal = "Nitric Oxide: Biology and Chemistry",
title = "A high fat diet induces sex-specific differences in hepatic lipid metabolism and nitrite/nitrate in rats",
volume = "54",
pages = "51-59",
doi = "10.1016/j.niox.2016.02.007"
}

Stanimirović, J., Obradović, M. M., Jovanović, A., Sudar, E., Zafirović, S., Pitt, S. J., Stewart, A. J.,& Isenović, E. R.. (2016). A high fat diet induces sex-specific differences in hepatic lipid metabolism and nitrite/nitrate in rats. in Nitric Oxide: Biology and Chemistry
Elsevier., 54, 51-59.
https://doi.org/10.1016/j.niox.2016.02.007

Stanimirović J, Obradović MM, Jovanović A, Sudar E, Zafirović S, Pitt SJ, Stewart AJ, Isenović ER. A high fat diet induces sex-specific differences in hepatic lipid metabolism and nitrite/nitrate in rats. in Nitric Oxide: Biology and Chemistry. 2016;54:51-59.
doi:10.1016/j.niox.2016.02.007 .

Stanimirović, Julijana, Obradović, Milan M., Jovanović, Aleksandra, Sudar, Emina, Zafirović, Sonja, Pitt, Samantha J., Stewart, Alan J., Isenović, Esma R., "A high fat diet induces sex-specific differences in hepatic lipid metabolism and nitrite/nitrate in rats" in Nitric Oxide: Biology and Chemistry, 54 (2016):51-59,
https://doi.org/10.1016/j.niox.2016.02.007 . .

TY  - JOUR
AU  - Obradović, Milan M.
AU  - Stewart, Alan J.
AU  - Pitt, Samantha J.
AU  - Labudović-Borović, Milica
AU  - Sudar, Emina
AU  - Petrovic, Voin
AU  - Zafirović, Sonja
AU  - Maravić-Stojković, Vera
AU  - Vasić, Vesna M.
AU  - Isenović, Esma R.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5989
AB  - In this study the in vivo effects of estradiol in regulating Na+/K+-ATPase function in rat heart was studied. Adult male Wistar rats were treated with estradiol (40 mu g/kg, i.p.) and after 24 h the animals were sacrificed and the heart excised. Following estradiol administration, cardiac Na+/K(+)ATPase activity, expression of the alpha l subunit, and phosphorylation of the al subunit were significantly increased. These animals also had significantly decreased levels of digoxin-like immunoreactive factor(s). Na+ levels were also significantly reduced but to a level that was still within the normal physiological range, highlighting the ability of the Na+/K+-ATPase to balance the ionic composition following treatment with estradiol. Estradiol treated rats also showed increased phosphorylation of protein kinase B (Akt), and extracellular-signal-regulated kinase 1/2 (ERK1/2). We therefore suggest a role for Akt and/or ERK1/2 in estradiol-mediated regulation of cardiac Na+/K+-ATPase expression and activity in rat heart. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
T2  - Molecular and Cellular Endocrinology
T1  - In vivo effects of 17 beta-estradiol on cardiac Na+/K+-ATPase expression and activity in rat heart
VL  - 388
IS  - 1-2
SP  - 58
EP  - 68
DO  - 10.1016/j.mce.2014.03.005
ER  - 

@article{
author = "Obradović, Milan M. and Stewart, Alan J. and Pitt, Samantha J. and Labudović-Borović, Milica and Sudar, Emina and Petrovic, Voin and Zafirović, Sonja and Maravić-Stojković, Vera and Vasić, Vesna M. and Isenović, Esma R.",
year = "2014",
abstract = "In this study the in vivo effects of estradiol in regulating Na+/K+-ATPase function in rat heart was studied. Adult male Wistar rats were treated with estradiol (40 mu g/kg, i.p.) and after 24 h the animals were sacrificed and the heart excised. Following estradiol administration, cardiac Na+/K(+)ATPase activity, expression of the alpha l subunit, and phosphorylation of the al subunit were significantly increased. These animals also had significantly decreased levels of digoxin-like immunoreactive factor(s). Na+ levels were also significantly reduced but to a level that was still within the normal physiological range, highlighting the ability of the Na+/K+-ATPase to balance the ionic composition following treatment with estradiol. Estradiol treated rats also showed increased phosphorylation of protein kinase B (Akt), and extracellular-signal-regulated kinase 1/2 (ERK1/2). We therefore suggest a role for Akt and/or ERK1/2 in estradiol-mediated regulation of cardiac Na+/K+-ATPase expression and activity in rat heart. (C) 2014 Elsevier Ireland Ltd. All rights reserved.",
journal = "Molecular and Cellular Endocrinology",
title = "In vivo effects of 17 beta-estradiol on cardiac Na+/K+-ATPase expression and activity in rat heart",
volume = "388",
number = "1-2",
pages = "58-68",
doi = "10.1016/j.mce.2014.03.005"
}

Obradović, M. M., Stewart, A. J., Pitt, S. J., Labudović-Borović, M., Sudar, E., Petrovic, V., Zafirović, S., Maravić-Stojković, V., Vasić, V. M.,& Isenović, E. R.. (2014). In vivo effects of 17 beta-estradiol on cardiac Na+/K+-ATPase expression and activity in rat heart. in Molecular and Cellular Endocrinology, 388(1-2), 58-68.
https://doi.org/10.1016/j.mce.2014.03.005

Obradović MM, Stewart AJ, Pitt SJ, Labudović-Borović M, Sudar E, Petrovic V, Zafirović S, Maravić-Stojković V, Vasić VM, Isenović ER. In vivo effects of 17 beta-estradiol on cardiac Na+/K+-ATPase expression and activity in rat heart. in Molecular and Cellular Endocrinology. 2014;388(1-2):58-68.
doi:10.1016/j.mce.2014.03.005 .

Obradović, Milan M., Stewart, Alan J., Pitt, Samantha J., Labudović-Borović, Milica, Sudar, Emina, Petrovic, Voin, Zafirović, Sonja, Maravić-Stojković, Vera, Vasić, Vesna M., Isenović, Esma R., "In vivo effects of 17 beta-estradiol on cardiac Na+/K+-ATPase expression and activity in rat heart" in Molecular and Cellular Endocrinology, 388, no. 1-2 (2014):58-68,
https://doi.org/10.1016/j.mce.2014.03.005 . .