TY  - JOUR
AU  - Singh, Jagtar
AU  - Thachil, Thanuja
AU  - Misir, Sema
AU  - Altay, Diler
AU  - Yaman, Serap
AU  - Singh, Gurpreet
AU  - Eapen, Mathew
AU  - McAlinden, Kielan
AU  - Petrović, Nina
AU  - Sohal, Sukhwinder
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13133
AB  - Radiation‑induced normal tissue toxicity is a common acute and chronic outcome of radiotherapy (RT) for prostate cancer (PCa). There are currently no existing pre‑assessments before treatment to predict acute and late RT‑induced toxicity. Novel predictive blood biomarkers in radiation oncology may improve treatment decision‑making and therapeutic monitoring for patients with PCa. A comprehensive systematic search of microRNA (miRNA/miR) profiling studies published in PubMed, Science Direct and Google Scholar was performed. The present systematic review, supported by meta‑analysis, summarises key findings and discusses the results of prospective clinical studies investigating miRNA expression levels and their association with RT‑induced toxicity in PCa. Out of seven clinical studies, six highlighted levels of 10 miRNAs changing in plasma, serum or peripheral blood mononuclear cells during RT. The post‑RT expression levels of miRNA‑132‑5p, miRNA‑1‑3p, miRNA‑410 and miRNA‑221 were increased, and miRNA‑23a‑3p expression was decreased among patients with low‑grade RT‑induced toxicity. Furthermore, in patients with high‑grade RT toxicity, miRNA‑197‑3p, miRNA‑151a‑5p, miRNA‑18b‑5p, miRNA‑99a and miRNA‑21 expression was increased, while miRNA‑132‑5p expression was decreased. The present miRNA meta‑analysis could be the focus of future studies to identify their potential clinical value as PCa biomarkers and therapeutic mediators in RT‑induced toxicity. The variations in miRNA levels post‑RT could be used as predictive biomarkers of RT‑induced toxicity. However, further extensive validation is required to establish the relationship between miRNA expression levels and RT‑induced toxicity in PCa and to confirm their predictive value.
T2  - World Academy of Sciences Journal
T1  - Expression of microRNAs following radiation therapy and association with severity of radiotherapy‑induced toxicity among patients with prostate adenocarcinoma: A systematic review and meta‑analysis
VL  - 6
IS  - 3
DO  - 10.3892/wasj.2024.242
ER  - 

@article{
author = "Singh, Jagtar and Thachil, Thanuja and Misir, Sema and Altay, Diler and Yaman, Serap and Singh, Gurpreet and Eapen, Mathew and McAlinden, Kielan and Petrović, Nina and Sohal, Sukhwinder",
year = "2024",
abstract = "Radiation‑induced normal tissue toxicity is a common acute and chronic outcome of radiotherapy (RT) for prostate cancer (PCa). There are currently no existing pre‑assessments before treatment to predict acute and late RT‑induced toxicity. Novel predictive blood biomarkers in radiation oncology may improve treatment decision‑making and therapeutic monitoring for patients with PCa. A comprehensive systematic search of microRNA (miRNA/miR) profiling studies published in PubMed, Science Direct and Google Scholar was performed. The present systematic review, supported by meta‑analysis, summarises key findings and discusses the results of prospective clinical studies investigating miRNA expression levels and their association with RT‑induced toxicity in PCa. Out of seven clinical studies, six highlighted levels of 10 miRNAs changing in plasma, serum or peripheral blood mononuclear cells during RT. The post‑RT expression levels of miRNA‑132‑5p, miRNA‑1‑3p, miRNA‑410 and miRNA‑221 were increased, and miRNA‑23a‑3p expression was decreased among patients with low‑grade RT‑induced toxicity. Furthermore, in patients with high‑grade RT toxicity, miRNA‑197‑3p, miRNA‑151a‑5p, miRNA‑18b‑5p, miRNA‑99a and miRNA‑21 expression was increased, while miRNA‑132‑5p expression was decreased. The present miRNA meta‑analysis could be the focus of future studies to identify their potential clinical value as PCa biomarkers and therapeutic mediators in RT‑induced toxicity. The variations in miRNA levels post‑RT could be used as predictive biomarkers of RT‑induced toxicity. However, further extensive validation is required to establish the relationship between miRNA expression levels and RT‑induced toxicity in PCa and to confirm their predictive value.",
journal = "World Academy of Sciences Journal",
title = "Expression of microRNAs following radiation therapy and association with severity of radiotherapy‑induced toxicity among patients with prostate adenocarcinoma: A systematic review and meta‑analysis",
volume = "6",
number = "3",
doi = "10.3892/wasj.2024.242"
}

Singh, J., Thachil, T., Misir, S., Altay, D., Yaman, S., Singh, G., Eapen, M., McAlinden, K., Petrović, N.,& Sohal, S.. (2024). Expression of microRNAs following radiation therapy and association with severity of radiotherapy‑induced toxicity among patients with prostate adenocarcinoma: A systematic review and meta‑analysis. in World Academy of Sciences Journal, 6(3).
https://doi.org/10.3892/wasj.2024.242

Singh J, Thachil T, Misir S, Altay D, Yaman S, Singh G, Eapen M, McAlinden K, Petrović N, Sohal S. Expression of microRNAs following radiation therapy and association with severity of radiotherapy‑induced toxicity among patients with prostate adenocarcinoma: A systematic review and meta‑analysis. in World Academy of Sciences Journal. 2024;6(3).
doi:10.3892/wasj.2024.242 .

Singh, Jagtar, Thachil, Thanuja, Misir, Sema, Altay, Diler, Yaman, Serap, Singh, Gurpreet, Eapen, Mathew, McAlinden, Kielan, Petrović, Nina, Sohal, Sukhwinder, "Expression of microRNAs following radiation therapy and association with severity of radiotherapy‑induced toxicity among patients with prostate adenocarcinoma: A systematic review and meta‑analysis" in World Academy of Sciences Journal, 6, no. 3 (2024),
https://doi.org/10.3892/wasj.2024.242 . .

TY  - JOUR
AU  - Preljević, Kenan
AU  - Pašić, Ivana
AU  - Vlaović, Milorad
AU  - Matić, Ivana Z.
AU  - Krivokapić, Slađana
AU  - Petrović, Nina
AU  - Stanojković, Tatjana
AU  - Živković, Vladimir
AU  - Perović, Svetlana
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12935
AB  - The essential oils of Thymus vulgaris (TVEO) and Thymus serpyllum (TSEO) show different biological activities. The aim of the study was to evaluate the biological activities of TVEO and TSEO from Montenegro. The main components of TVEO were p-cymene (29.52%), thymol (22.8%) and linalool (4.73%) while the main components of TSEO were p-cymene (19.04%), geraniol (11,09%), linalool (9.16%), geranyl acetate (6.49%) and borneol (5.24%). Antioxidant activity determined via DPPH for TVEO was 4.49 and FRAP 1130.27, while for TSEO it was estimated that DPPH was 4.88 μL/mL and FRAP was 701.25 μmol FRAP/L. Both essential oils were active against all tested bacteria, with the highest level of sensitivity of E. coli with MIC of 1.5625 μL/mL. Essential oils showed strong cytotoxic effects on human cancer cell lines, with IC50 values ranging from 0.20 to 0.24 μL/mL for TVEO and from 0.32 to 0.49 μL/mL for TSEO. TVEO caused apoptosis in cervical adenocarcinoma HeLa cells through activation of caspase-3 and caspase-8, while TSEO caused apoptosis through caspase-3. EOs decreased levels of oxidative stress in normal MRC-5 cells. HeLa cells treated with TVEO had reduced MMP2 expression levels, while cells treated with TSEO had lowered MMP2 and MMP9 levels. The treatment of HeLa cells with TVEO increased the levels of miR-16 and miR-34a, indicating potential tumor-suppressive properties. Our findings suggest that Thymus essential oils may be considered as good candidates for further investigation as cancer-chemopreventive and cancer-therapeutic agents.
T2  - Fitoterapia
T1  - Comparative analysis of chemical profiles, antioxidant, antibacterial, and anticancer effects of essential oils of two Thymus species from Montenegro
VL  - 174
SP  - 105871
DO  - 10.1016/j.fitote.2024.105871
ER  - 

@article{
author = "Preljević, Kenan and Pašić, Ivana and Vlaović, Milorad and Matić, Ivana Z. and Krivokapić, Slađana and Petrović, Nina and Stanojković, Tatjana and Živković, Vladimir and Perović, Svetlana",
year = "2024",
abstract = "The essential oils of Thymus vulgaris (TVEO) and Thymus serpyllum (TSEO) show different biological activities. The aim of the study was to evaluate the biological activities of TVEO and TSEO from Montenegro. The main components of TVEO were p-cymene (29.52%), thymol (22.8%) and linalool (4.73%) while the main components of TSEO were p-cymene (19.04%), geraniol (11,09%), linalool (9.16%), geranyl acetate (6.49%) and borneol (5.24%). Antioxidant activity determined via DPPH for TVEO was 4.49 and FRAP 1130.27, while for TSEO it was estimated that DPPH was 4.88 μL/mL and FRAP was 701.25 μmol FRAP/L. Both essential oils were active against all tested bacteria, with the highest level of sensitivity of E. coli with MIC of 1.5625 μL/mL. Essential oils showed strong cytotoxic effects on human cancer cell lines, with IC50 values ranging from 0.20 to 0.24 μL/mL for TVEO and from 0.32 to 0.49 μL/mL for TSEO. TVEO caused apoptosis in cervical adenocarcinoma HeLa cells through activation of caspase-3 and caspase-8, while TSEO caused apoptosis through caspase-3. EOs decreased levels of oxidative stress in normal MRC-5 cells. HeLa cells treated with TVEO had reduced MMP2 expression levels, while cells treated with TSEO had lowered MMP2 and MMP9 levels. The treatment of HeLa cells with TVEO increased the levels of miR-16 and miR-34a, indicating potential tumor-suppressive properties. Our findings suggest that Thymus essential oils may be considered as good candidates for further investigation as cancer-chemopreventive and cancer-therapeutic agents.",
journal = "Fitoterapia",
title = "Comparative analysis of chemical profiles, antioxidant, antibacterial, and anticancer effects of essential oils of two Thymus species from Montenegro",
volume = "174",
pages = "105871",
doi = "10.1016/j.fitote.2024.105871"
}

Preljević, K., Pašić, I., Vlaović, M., Matić, I. Z., Krivokapić, S., Petrović, N., Stanojković, T., Živković, V.,& Perović, S.. (2024). Comparative analysis of chemical profiles, antioxidant, antibacterial, and anticancer effects of essential oils of two Thymus species from Montenegro. in Fitoterapia, 174, 105871.
https://doi.org/10.1016/j.fitote.2024.105871

Preljević K, Pašić I, Vlaović M, Matić IZ, Krivokapić S, Petrović N, Stanojković T, Živković V, Perović S. Comparative analysis of chemical profiles, antioxidant, antibacterial, and anticancer effects of essential oils of two Thymus species from Montenegro. in Fitoterapia. 2024;174:105871.
doi:10.1016/j.fitote.2024.105871 .

Preljević, Kenan, Pašić, Ivana, Vlaović, Milorad, Matić, Ivana Z., Krivokapić, Slađana, Petrović, Nina, Stanojković, Tatjana, Živković, Vladimir, Perović, Svetlana, "Comparative analysis of chemical profiles, antioxidant, antibacterial, and anticancer effects of essential oils of two Thymus species from Montenegro" in Fitoterapia, 174 (2024):105871,
https://doi.org/10.1016/j.fitote.2024.105871 . .

TY  - CONF
AU  - Pašić, Ivana
AU  - Preljević, Kenan
AU  - Matić, Ivana
AU  - Petrović, Nina
AU  - Stanojković, Tatjana
AU  - Perović, Svetlana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12566
AB  - Background: It has been demonstrated that aromaƟ c plant essenƟ al oils (EO) contain phytochemicals with anƟ - infl ammatory, anƟ oxidant, and anƟ cancer acƟ viƟ es. The goal of this study was to examine the cytotoxicity and the anƟ cancer acƟ on mechanisms of an EO extracted from Satureja montana plant species, originaƟ ng from Montenegro. Material and methods: The cytotoxic acƟ vity was assessed against human cancer cell lines: cervical adenocarcinoma HeLa, malignant melanoma A375, colorectal adenocarcinoma LS 174T, lung carcinoma A549, as well as against normal human lung fi broblasts MRC-5 by MTT assay. Using fl ow cytometry, it was examined how HeLa cells were distributed throughout the cell cycle aŌ er treatment with EO and whether caspase-3, caspase-8, and caspase-9 were acƟ vated. RT-qPCR was used to assess the expression levels of genes and miRNA in HeLa cells. Results: Satureja montana EO exerted strong cytotoxicity against human cancer cell lines, with IC50 values ranging from 0,12 to 0,18 µL/mL, The strongest cytotoxic acƟ vity of EO was observed against lung carcinoma A549 cells with an IC50 value of 0.12 µL/mL and LS 174T colorectal adenocarcinoma cells with IC50 value of 0.13 µL/mL. The lowest cytotoxicity was determined against normal lung fi broblasts MRC-5 (IC50 value of 0.19 µL/mL). AŌ er 24 hours of treatment with Satureja montana EO, a striking increase in the proporƟ on of HeLa cells in the subG1 phase was seen in comparison with control cells. Pretreatment of HeLa cells with caspase inhibitors showed that Satureja montana EO acƟ vated all three invesƟ gated caspases to trigger apoptosis. In comparison to control cells, Satureja montana EO treatment of HeLa cells decreased MMP2 gene expression levels, and elevated MMP9 and VEGFA gene expression levels. Satureja montana EO treatment increased the expression levels of the tumor supressive miR-16 and miR-34ain HeLa cells, and increased levels of miR21, with oncogenic role in cervical cancer. Conclusion: Satureja montana EO showed strong cytotoxic eff ects on tested cancer cells. EO exerted prominent apoptoƟ c acƟ vity in HeLa cells via intrinsic and extrinsic pathways. Decrease in the expression levels of MMP2 gene, involved in invasion and metastasis, and cancer suppressive miRNAs, further support its potenƟ al to be uƟ lized as a therapeuƟ c agent in cancer treatment.
PB  - Belgrade : Serbian Association for Cancer Research
C3  - Oncology Insights
T1  - Exploring the anticancer activity of essential oil of Satureja montana L. from Montenegro
IS  - 1
SP  - 67
EP  - 68
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12566
ER  - 

@conference{
author = "Pašić, Ivana and Preljević, Kenan and Matić, Ivana and Petrović, Nina and Stanojković, Tatjana and Perović, Svetlana",
year = "2023",
abstract = "Background: It has been demonstrated that aromaƟ c plant essenƟ al oils (EO) contain phytochemicals with anƟ - infl ammatory, anƟ oxidant, and anƟ cancer acƟ viƟ es. The goal of this study was to examine the cytotoxicity and the anƟ cancer acƟ on mechanisms of an EO extracted from Satureja montana plant species, originaƟ ng from Montenegro. Material and methods: The cytotoxic acƟ vity was assessed against human cancer cell lines: cervical adenocarcinoma HeLa, malignant melanoma A375, colorectal adenocarcinoma LS 174T, lung carcinoma A549, as well as against normal human lung fi broblasts MRC-5 by MTT assay. Using fl ow cytometry, it was examined how HeLa cells were distributed throughout the cell cycle aŌ er treatment with EO and whether caspase-3, caspase-8, and caspase-9 were acƟ vated. RT-qPCR was used to assess the expression levels of genes and miRNA in HeLa cells. Results: Satureja montana EO exerted strong cytotoxicity against human cancer cell lines, with IC50 values ranging from 0,12 to 0,18 µL/mL, The strongest cytotoxic acƟ vity of EO was observed against lung carcinoma A549 cells with an IC50 value of 0.12 µL/mL and LS 174T colorectal adenocarcinoma cells with IC50 value of 0.13 µL/mL. The lowest cytotoxicity was determined against normal lung fi broblasts MRC-5 (IC50 value of 0.19 µL/mL). AŌ er 24 hours of treatment with Satureja montana EO, a striking increase in the proporƟ on of HeLa cells in the subG1 phase was seen in comparison with control cells. Pretreatment of HeLa cells with caspase inhibitors showed that Satureja montana EO acƟ vated all three invesƟ gated caspases to trigger apoptosis. In comparison to control cells, Satureja montana EO treatment of HeLa cells decreased MMP2 gene expression levels, and elevated MMP9 and VEGFA gene expression levels. Satureja montana EO treatment increased the expression levels of the tumor supressive miR-16 and miR-34ain HeLa cells, and increased levels of miR21, with oncogenic role in cervical cancer. Conclusion: Satureja montana EO showed strong cytotoxic eff ects on tested cancer cells. EO exerted prominent apoptoƟ c acƟ vity in HeLa cells via intrinsic and extrinsic pathways. Decrease in the expression levels of MMP2 gene, involved in invasion and metastasis, and cancer suppressive miRNAs, further support its potenƟ al to be uƟ lized as a therapeuƟ c agent in cancer treatment.",
publisher = "Belgrade : Serbian Association for Cancer Research",
journal = "Oncology Insights",
title = "Exploring the anticancer activity of essential oil of Satureja montana L. from Montenegro",
number = "1",
pages = "67-68",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12566"
}

Pašić, I., Preljević, K., Matić, I., Petrović, N., Stanojković, T.,& Perović, S.. (2023). Exploring the anticancer activity of essential oil of Satureja montana L. from Montenegro. in Oncology Insights
Belgrade : Serbian Association for Cancer Research.(1), 67-68.
https://hdl.handle.net/21.15107/rcub_vinar_12566

Pašić I, Preljević K, Matić I, Petrović N, Stanojković T, Perović S. Exploring the anticancer activity of essential oil of Satureja montana L. from Montenegro. in Oncology Insights. 2023;(1):67-68.
https://hdl.handle.net/21.15107/rcub_vinar_12566 .

Pašić, Ivana, Preljević, Kenan, Matić, Ivana, Petrović, Nina, Stanojković, Tatjana, Perović, Svetlana, "Exploring the anticancer activity of essential oil of Satureja montana L. from Montenegro" in Oncology Insights, no. 1 (2023):67-68,
https://hdl.handle.net/21.15107/rcub_vinar_12566 .

TY  - JOUR
AU  - Kokanov, Nikola
AU  - Jovanović-Ćupić, Snežana
AU  - Šiljić, Marina
AU  - Ćirković, Valentina
AU  - Petrović, Nina
AU  - Kožik, Bojana
AU  - Krajnović, Milena
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12051
AB  - Variations in the hepatitis C virus (HCV) core sequence have been related to disease progression and response to antiviral therapy. Previously we showed that the methylation status of RASSF1A and p16 genes, and IL28B genotypes affects the response to pegylated interferon/ribavirin (PEG-IFN/RBV) therapy. Herein we investigated whether amino acid (aa) substitutions in the HCV core region alone or in combination with IL28B genotypes and RASSF1A/p16 methylation affect the response to PEG-IFN/RBV therapy and liver disease progression. Among 29 examined patients, we found no association between single aa substitutions and response to therapy. However, we observed that patients with the HCV core aa substitution at position 75 and CT/TT IL28B genotypes were non-responders (NR), (P=0.023). Moreover, these patients had unmethylated RASSF1A. In contrast, most patients (75%) with aa substitutions at position 91 and CC IL28B genotype achieved sustained virologic response (SVR), (P=0.030), and 70% of them had methylated RASSF1A gene. Our results suggest that combined analysis of aa substitutions in the core protein, the IL28B rs12979860 polymorphism, and the methylation status of the RASSF1A gene may help in predicting treatment response to PEG-IFN/RBV in genotype 1b chronic hepatitis C patients.
T2  - Archives of Biological Sciences
T1  - Variability of the HCV core region and host genetic and epigenetic factors can predict the response to pegylated interferon/ribavirin therapy in genotype 1b hepatitis C patients from Serbia
VL  - 75
IS  - 3
SP  - 251
EP  - 262
DO  - 10.2298/ABS230316020K
ER  - 

@article{
author = "Kokanov, Nikola and Jovanović-Ćupić, Snežana and Šiljić, Marina and Ćirković, Valentina and Petrović, Nina and Kožik, Bojana and Krajnović, Milena",
year = "2023",
abstract = "Variations in the hepatitis C virus (HCV) core sequence have been related to disease progression and response to antiviral therapy. Previously we showed that the methylation status of RASSF1A and p16 genes, and IL28B genotypes affects the response to pegylated interferon/ribavirin (PEG-IFN/RBV) therapy. Herein we investigated whether amino acid (aa) substitutions in the HCV core region alone or in combination with IL28B genotypes and RASSF1A/p16 methylation affect the response to PEG-IFN/RBV therapy and liver disease progression. Among 29 examined patients, we found no association between single aa substitutions and response to therapy. However, we observed that patients with the HCV core aa substitution at position 75 and CT/TT IL28B genotypes were non-responders (NR), (P=0.023). Moreover, these patients had unmethylated RASSF1A. In contrast, most patients (75%) with aa substitutions at position 91 and CC IL28B genotype achieved sustained virologic response (SVR), (P=0.030), and 70% of them had methylated RASSF1A gene. Our results suggest that combined analysis of aa substitutions in the core protein, the IL28B rs12979860 polymorphism, and the methylation status of the RASSF1A gene may help in predicting treatment response to PEG-IFN/RBV in genotype 1b chronic hepatitis C patients.",
journal = "Archives of Biological Sciences",
title = "Variability of the HCV core region and host genetic and epigenetic factors can predict the response to pegylated interferon/ribavirin therapy in genotype 1b hepatitis C patients from Serbia",
volume = "75",
number = "3",
pages = "251-262",
doi = "10.2298/ABS230316020K"
}

Kokanov, N., Jovanović-Ćupić, S., Šiljić, M., Ćirković, V., Petrović, N., Kožik, B.,& Krajnović, M.. (2023). Variability of the HCV core region and host genetic and epigenetic factors can predict the response to pegylated interferon/ribavirin therapy in genotype 1b hepatitis C patients from Serbia. in Archives of Biological Sciences, 75(3), 251-262.
https://doi.org/10.2298/ABS230316020K

Kokanov N, Jovanović-Ćupić S, Šiljić M, Ćirković V, Petrović N, Kožik B, Krajnović M. Variability of the HCV core region and host genetic and epigenetic factors can predict the response to pegylated interferon/ribavirin therapy in genotype 1b hepatitis C patients from Serbia. in Archives of Biological Sciences. 2023;75(3):251-262.
doi:10.2298/ABS230316020K .

Kokanov, Nikola, Jovanović-Ćupić, Snežana, Šiljić, Marina, Ćirković, Valentina, Petrović, Nina, Kožik, Bojana, Krajnović, Milena, "Variability of the HCV core region and host genetic and epigenetic factors can predict the response to pegylated interferon/ribavirin therapy in genotype 1b hepatitis C patients from Serbia" in Archives of Biological Sciences, 75, no. 3 (2023):251-262,
https://doi.org/10.2298/ABS230316020K . .

TY  - JOUR
AU  - Barać, Milena
AU  - Petrović, Milan
AU  - Petrović, Nina
AU  - Nikolić-Jakoba, Nataša
AU  - Aleksić, Zoran
AU  - Todorović, Lidija
AU  - Petrović-Stanojević, Nataša
AU  - Anđelić-Jelić, Marina
AU  - Davidović, Aleksandar
AU  - Milašin, Jelena
AU  - Roganović, Jelena
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11499
AB  - Type 2 diabetes mellitus (T2DM) is associated with functional deterioration of the salivary gland and dental pulp, related to oxidative stress. The aim was to integrate experimental and bioinformatic findings to analyze the cellular mechanism of melatonin (MEL) action in the human parotid gland and dental pulp in diabetes. Human parotid gland tissue was obtained from 16 non-diabetic and 16 diabetic participants, as well as human dental pulp from 15 non-diabetic and 15 diabetic participants. In human non-diabetic and diabetic parotid gland cells (hPGCs) as well as in dental pulp cells (hDPCs), cultured in hyper- and normoglycemic conditions, glial cell linederived neurotrophic factor (GDNF), MEL, inducible nitric oxide synthase (iNOS) protein expression, and superoxide dismutase (SOD) activity were measured by enzyme-linked immunosorbent assay (ELISA) and spectrophotometrically. Bioinformatic analysis was performed using ShinyGO (v.0.75) application. Diabetic participants had increased GDNF and decreased MEL in parotid (p < 0.01) and dental pulp (p < 0.05) tissues, associated with increased iNOS and SOD activity. Normoglycemic hDPCs and non-diabetic hPGCs treated with 0.1 mM MEL had increased GDNF (p < 0.05), while hyperglycemic hDPCs treated with 1 mM MEL showed a decrease in up-regulated GDNF (p < 0.05). Enrichment analyses showed interference with stress and ATF/CREB signaling. MEL induced the stress-protective mechanism in hyperglycemic hDPCs and diabetic hPGCs, suggesting MEL could be beneficial for diabetes-associated disturbances in oral tissues.
T2  - International Journal of Environmental Research and Public Health
T1  - Melatonin Action in Type 2 Diabetic Parotid Gland and Dental Pulp: In Vitro and Bioinformatic Findings
VL  - 20
IS  - 18
SP  - 6727
DO  - 10.3390/ijerph20186727
ER  - 

@article{
author = "Barać, Milena and Petrović, Milan and Petrović, Nina and Nikolić-Jakoba, Nataša and Aleksić, Zoran and Todorović, Lidija and Petrović-Stanojević, Nataša and Anđelić-Jelić, Marina and Davidović, Aleksandar and Milašin, Jelena and Roganović, Jelena",
year = "2023",
abstract = "Type 2 diabetes mellitus (T2DM) is associated with functional deterioration of the salivary gland and dental pulp, related to oxidative stress. The aim was to integrate experimental and bioinformatic findings to analyze the cellular mechanism of melatonin (MEL) action in the human parotid gland and dental pulp in diabetes. Human parotid gland tissue was obtained from 16 non-diabetic and 16 diabetic participants, as well as human dental pulp from 15 non-diabetic and 15 diabetic participants. In human non-diabetic and diabetic parotid gland cells (hPGCs) as well as in dental pulp cells (hDPCs), cultured in hyper- and normoglycemic conditions, glial cell linederived neurotrophic factor (GDNF), MEL, inducible nitric oxide synthase (iNOS) protein expression, and superoxide dismutase (SOD) activity were measured by enzyme-linked immunosorbent assay (ELISA) and spectrophotometrically. Bioinformatic analysis was performed using ShinyGO (v.0.75) application. Diabetic participants had increased GDNF and decreased MEL in parotid (p < 0.01) and dental pulp (p < 0.05) tissues, associated with increased iNOS and SOD activity. Normoglycemic hDPCs and non-diabetic hPGCs treated with 0.1 mM MEL had increased GDNF (p < 0.05), while hyperglycemic hDPCs treated with 1 mM MEL showed a decrease in up-regulated GDNF (p < 0.05). Enrichment analyses showed interference with stress and ATF/CREB signaling. MEL induced the stress-protective mechanism in hyperglycemic hDPCs and diabetic hPGCs, suggesting MEL could be beneficial for diabetes-associated disturbances in oral tissues.",
journal = "International Journal of Environmental Research and Public Health",
title = "Melatonin Action in Type 2 Diabetic Parotid Gland and Dental Pulp: In Vitro and Bioinformatic Findings",
volume = "20",
number = "18",
pages = "6727",
doi = "10.3390/ijerph20186727"
}

Barać, M., Petrović, M., Petrović, N., Nikolić-Jakoba, N., Aleksić, Z., Todorović, L., Petrović-Stanojević, N., Anđelić-Jelić, M., Davidović, A., Milašin, J.,& Roganović, J.. (2023). Melatonin Action in Type 2 Diabetic Parotid Gland and Dental Pulp: In Vitro and Bioinformatic Findings. in International Journal of Environmental Research and Public Health, 20(18), 6727.
https://doi.org/10.3390/ijerph20186727

Barać M, Petrović M, Petrović N, Nikolić-Jakoba N, Aleksić Z, Todorović L, Petrović-Stanojević N, Anđelić-Jelić M, Davidović A, Milašin J, Roganović J. Melatonin Action in Type 2 Diabetic Parotid Gland and Dental Pulp: In Vitro and Bioinformatic Findings. in International Journal of Environmental Research and Public Health. 2023;20(18):6727.
doi:10.3390/ijerph20186727 .

Barać, Milena, Petrović, Milan, Petrović, Nina, Nikolić-Jakoba, Nataša, Aleksić, Zoran, Todorović, Lidija, Petrović-Stanojević, Nataša, Anđelić-Jelić, Marina, Davidović, Aleksandar, Milašin, Jelena, Roganović, Jelena, "Melatonin Action in Type 2 Diabetic Parotid Gland and Dental Pulp: In Vitro and Bioinformatic Findings" in International Journal of Environmental Research and Public Health, 20, no. 18 (2023):6727,
https://doi.org/10.3390/ijerph20186727 . .

TY  - JOUR
AU  - Petrović, Nina
AU  - Essack, Magbubah
AU  - Šami, Ahmad
AU  - Perry, George
AU  - Gojobori, Takashi
AU  - Isenović, Esma R.
AU  - Bajić, Vladan P.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11351
AB  - MicroRNAs (miRNAs) are involved in the regulation of various cellular processes including pathological conditions. MiRNA networks have been extensively researched in age-related degenerative diseases, such as cancer, Alzheimer’s disease (AD), and heart failure. Thus, miRNA has been studied from different approaches, in vivo, in vitro, and in silico including miRNA networks. Networks linking diverse biomedical entities unveil information not readily observable by other means. This work focuses on biological networks related to Breast cancer susceptibility 1 (BRCA1) in AD and breast cancer (BC). Using various bioinformatics approaches, we identified subnetworks common to AD and BC that suggest they are linked. According to our results, miR-107 was identified as a potentially good candidate for both AD and BC treatment (targeting BRCA1/2 and PTEN in both diseases), accompanied by miR-146a and miR-17. The analysis also confirmed the involvement of the miR-17-92 cluster, and miR-124-3p, and highlighted the importance of poorly researched miRNAs such as mir-6785 mir6127, mir-6870, or miR-8485. After filtering the in silico analysis results, we found 49 miRNA molecules that modulate the expression of at least five genes common to both BC and AD. Those 49 miRNAs regulate the expression of 122 genes in AD and 93 genes in BC, from which 26 genes are common genes for AD and BC involved in neuron differentiation and genesis, cell differentiation and migration, regulation of cell cycle, and cancer development. Additionally, the highly enriched pathway was associated with diabetic complications, pointing out possible interplay among molecules underlying BC, AD, and diabetes pathology
T2  - Computational Biology and Chemistry
T1  - MicroRNA networks linked with BRCA1/2, PTEN, and common genes for Alzheimer's disease and breast cancer share highly enriched pathways that may unravel targets for the AD/BC comorbidity treatment
VL  - 106
SP  - 107925
DO  - 10.1016/j.compbiolchem.2023.107925
ER  - 

@article{
author = "Petrović, Nina and Essack, Magbubah and Šami, Ahmad and Perry, George and Gojobori, Takashi and Isenović, Esma R. and Bajić, Vladan P.",
year = "2023",
abstract = "MicroRNAs (miRNAs) are involved in the regulation of various cellular processes including pathological conditions. MiRNA networks have been extensively researched in age-related degenerative diseases, such as cancer, Alzheimer’s disease (AD), and heart failure. Thus, miRNA has been studied from different approaches, in vivo, in vitro, and in silico including miRNA networks. Networks linking diverse biomedical entities unveil information not readily observable by other means. This work focuses on biological networks related to Breast cancer susceptibility 1 (BRCA1) in AD and breast cancer (BC). Using various bioinformatics approaches, we identified subnetworks common to AD and BC that suggest they are linked. According to our results, miR-107 was identified as a potentially good candidate for both AD and BC treatment (targeting BRCA1/2 and PTEN in both diseases), accompanied by miR-146a and miR-17. The analysis also confirmed the involvement of the miR-17-92 cluster, and miR-124-3p, and highlighted the importance of poorly researched miRNAs such as mir-6785 mir6127, mir-6870, or miR-8485. After filtering the in silico analysis results, we found 49 miRNA molecules that modulate the expression of at least five genes common to both BC and AD. Those 49 miRNAs regulate the expression of 122 genes in AD and 93 genes in BC, from which 26 genes are common genes for AD and BC involved in neuron differentiation and genesis, cell differentiation and migration, regulation of cell cycle, and cancer development. Additionally, the highly enriched pathway was associated with diabetic complications, pointing out possible interplay among molecules underlying BC, AD, and diabetes pathology",
journal = "Computational Biology and Chemistry",
title = "MicroRNA networks linked with BRCA1/2, PTEN, and common genes for Alzheimer's disease and breast cancer share highly enriched pathways that may unravel targets for the AD/BC comorbidity treatment",
volume = "106",
pages = "107925",
doi = "10.1016/j.compbiolchem.2023.107925"
}

Petrović, N., Essack, M., Šami, A., Perry, G., Gojobori, T., Isenović, E. R.,& Bajić, V. P.. (2023). MicroRNA networks linked with BRCA1/2, PTEN, and common genes for Alzheimer's disease and breast cancer share highly enriched pathways that may unravel targets for the AD/BC comorbidity treatment. in Computational Biology and Chemistry, 106, 107925.
https://doi.org/10.1016/j.compbiolchem.2023.107925

Petrović N, Essack M, Šami A, Perry G, Gojobori T, Isenović ER, Bajić VP. MicroRNA networks linked with BRCA1/2, PTEN, and common genes for Alzheimer's disease and breast cancer share highly enriched pathways that may unravel targets for the AD/BC comorbidity treatment. in Computational Biology and Chemistry. 2023;106:107925.
doi:10.1016/j.compbiolchem.2023.107925 .

Petrović, Nina, Essack, Magbubah, Šami, Ahmad, Perry, George, Gojobori, Takashi, Isenović, Esma R., Bajić, Vladan P., "MicroRNA networks linked with BRCA1/2, PTEN, and common genes for Alzheimer's disease and breast cancer share highly enriched pathways that may unravel targets for the AD/BC comorbidity treatment" in Computational Biology and Chemistry, 106 (2023):107925,
https://doi.org/10.1016/j.compbiolchem.2023.107925 . .

TY  - JOUR
AU  - Ergün, Sercan
AU  - Sankaranarayanan, Ramamoorthy
AU  - Petrović, Nina
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11568
AB  - COVID-19 is a viral respiratory infection induced by the newly discovered coronavirus SARS-CoV-2. miRNA is an example of a strong and direct regulator of a gene’s transcriptional activity. The interaction between miRNAs and their target molecules is responsible for homeostasis. Virus-derived and host-derived miRNAs are involved in the activity of hiding from immune system cells, inducing the inflammatory reaction through interplay with associated genes, during SARS-COV-2 infection. Interest in miRNAs has raised the comprehension of the machinery and pathophysiology of SARS-COV-2 infection. In this review, the effects and biological roles of miRNAs on SARS-CoV-2 pathogenicity and life cycle are described. The therapeutic potential of miRNAs against SARS-CoV-2 infection are also mentioned.
T2  - Epigenomics
T1  - Clinically informative microRNAs for SARS-CoV-2 infection
VL  - 15
IS  - 13
SP  - 705
EP  - 716
DO  - 10.2217/epi-2023-0179
ER  - 

@article{
author = "Ergün, Sercan and Sankaranarayanan, Ramamoorthy and Petrović, Nina",
year = "2023",
abstract = "COVID-19 is a viral respiratory infection induced by the newly discovered coronavirus SARS-CoV-2. miRNA is an example of a strong and direct regulator of a gene’s transcriptional activity. The interaction between miRNAs and their target molecules is responsible for homeostasis. Virus-derived and host-derived miRNAs are involved in the activity of hiding from immune system cells, inducing the inflammatory reaction through interplay with associated genes, during SARS-COV-2 infection. Interest in miRNAs has raised the comprehension of the machinery and pathophysiology of SARS-COV-2 infection. In this review, the effects and biological roles of miRNAs on SARS-CoV-2 pathogenicity and life cycle are described. The therapeutic potential of miRNAs against SARS-CoV-2 infection are also mentioned.",
journal = "Epigenomics",
title = "Clinically informative microRNAs for SARS-CoV-2 infection",
volume = "15",
number = "13",
pages = "705-716",
doi = "10.2217/epi-2023-0179"
}

Ergün, S., Sankaranarayanan, R.,& Petrović, N.. (2023). Clinically informative microRNAs for SARS-CoV-2 infection. in Epigenomics, 15(13), 705-716.
https://doi.org/10.2217/epi-2023-0179

Ergün S, Sankaranarayanan R, Petrović N. Clinically informative microRNAs for SARS-CoV-2 infection. in Epigenomics. 2023;15(13):705-716.
doi:10.2217/epi-2023-0179 .

Ergün, Sercan, Sankaranarayanan, Ramamoorthy, Petrović, Nina, "Clinically informative microRNAs for SARS-CoV-2 infection" in Epigenomics, 15, no. 13 (2023):705-716,
https://doi.org/10.2217/epi-2023-0179 . .

TY  - CONF
AU  - Petrović, Nina
AU  - Srbljak Ćuk, I.
AU  - Marjanovć Đorić, D.
AU  - Petrović, I.
AU  - Paunović, P.
AU  - Medić Milijić, Nataša
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12237
UR  - https://web.archive.org/web/20231218093802/https://nwm.covr.be/EACR2023abstracts/data/HtmlApp/main.html
AB  - Introduction  Nearly 60% of patients with breast cancer (BC) undergo radiotherapy (RT). A significant proportion of patients experience side effects that can influence their quality of life and treatment effectiveness. Gene and microRNA expression patterns may be associated with individual patients' sensitivity to RT. Material and Methods  Expression levels of miR-133b, miR-206, and their target genes matrix metalloproteinase 9 (MMP9) and vascular endothelial growth factor (VEGFA) were investigated in normal tissue adjacent to tumors of 27 BC patients. Sixteen out of 27 (59.3%) patients underwent RT, while 13 of them had breast skin reactions (acute dermatitis). Patients with acute dermatitis were divided into two groups, H-hypofractionated and F-standardly fractionated group. The H group underwent 16 fractions of RT (2.65 Gy), while the second group-F had 25 fractions (2 Gy) or 25 fractions plus boost. All patients from the F group had acute dermatitis before the 25th fraction, so the boost did not influence the time of toxicity occurrence. After the RNA extraction, followed by reverse transcription, expression levels of the four molecules were measured by RT-qPCR. Relative quantity (RQ) units were calculated by a comparative 2-ddCt method. Results and Discussions  MiR-133b/206 and MMP9/VEGFA gene levels did not differ between patients from H and F groups (p = 0.573; p = 0.143; p = 0.727; p = 0.582, respectively). The F group was divided into two subgroups-earlier (E) subgroup with patients with skin reactions before or at 20th fraction, and the later (L) subgroup with patients with skin reactions after 20th fraction. VEGFA gene expression was significantly higher in the E group compared with the L group, indicating that it may be a specific predictor of earlier onset of acute skin reaction in the normal breast tissue of patients undergoing RT (p = 0.036, Mann–Whitney U test). MicroRNAs miR-133b, miR-206, and MMP9 were not associated with earlier or later onset of skin reactions in this cohort (p = 0.343; p = 0.800; p = 0.786). It has been also shown that some VEGFA polymorphic regions can predict normal tissue reaction to RT in BC patients. Conclusion  Higher levels of VEGFA are associated with earlier onset of skin reactions, but in patients undergoing conventionally fractionated RT. VEGFA expression difference indicates that neoangiogenesis capacity might be associated with individual risk of earlier occurrence of acute side effects following RT.
PB  - EACR - European Association for Cancer Research
C3  - EACR 2023 Congress : Annual Congress of the European Association for Cancer Research : Abstracts
T1  - Higher VEGFA expression levels are associated with earlier onset of acute breast skin reactions of BC patients treated with radiotherapy
SP  - P-0422
EP  - P-0422
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12237
ER  - 

@conference{
author = "Petrović, Nina and Srbljak Ćuk, I. and Marjanovć Đorić, D. and Petrović, I. and Paunović, P. and Medić Milijić, Nataša",
year = "2023",
abstract = "Introduction  Nearly 60% of patients with breast cancer (BC) undergo radiotherapy (RT). A significant proportion of patients experience side effects that can influence their quality of life and treatment effectiveness. Gene and microRNA expression patterns may be associated with individual patients' sensitivity to RT. Material and Methods  Expression levels of miR-133b, miR-206, and their target genes matrix metalloproteinase 9 (MMP9) and vascular endothelial growth factor (VEGFA) were investigated in normal tissue adjacent to tumors of 27 BC patients. Sixteen out of 27 (59.3%) patients underwent RT, while 13 of them had breast skin reactions (acute dermatitis). Patients with acute dermatitis were divided into two groups, H-hypofractionated and F-standardly fractionated group. The H group underwent 16 fractions of RT (2.65 Gy), while the second group-F had 25 fractions (2 Gy) or 25 fractions plus boost. All patients from the F group had acute dermatitis before the 25th fraction, so the boost did not influence the time of toxicity occurrence. After the RNA extraction, followed by reverse transcription, expression levels of the four molecules were measured by RT-qPCR. Relative quantity (RQ) units were calculated by a comparative 2-ddCt method. Results and Discussions  MiR-133b/206 and MMP9/VEGFA gene levels did not differ between patients from H and F groups (p = 0.573; p = 0.143; p = 0.727; p = 0.582, respectively). The F group was divided into two subgroups-earlier (E) subgroup with patients with skin reactions before or at 20th fraction, and the later (L) subgroup with patients with skin reactions after 20th fraction. VEGFA gene expression was significantly higher in the E group compared with the L group, indicating that it may be a specific predictor of earlier onset of acute skin reaction in the normal breast tissue of patients undergoing RT (p = 0.036, Mann–Whitney U test). MicroRNAs miR-133b, miR-206, and MMP9 were not associated with earlier or later onset of skin reactions in this cohort (p = 0.343; p = 0.800; p = 0.786). It has been also shown that some VEGFA polymorphic regions can predict normal tissue reaction to RT in BC patients. Conclusion  Higher levels of VEGFA are associated with earlier onset of skin reactions, but in patients undergoing conventionally fractionated RT. VEGFA expression difference indicates that neoangiogenesis capacity might be associated with individual risk of earlier occurrence of acute side effects following RT.",
publisher = "EACR - European Association for Cancer Research",
journal = "EACR 2023 Congress : Annual Congress of the European Association for Cancer Research : Abstracts",
title = "Higher VEGFA expression levels are associated with earlier onset of acute breast skin reactions of BC patients treated with radiotherapy",
pages = "P-0422-P-0422",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12237"
}

Petrović, N., Srbljak Ćuk, I., Marjanovć Đorić, D., Petrović, I., Paunović, P.,& Medić Milijić, N.. (2023). Higher VEGFA expression levels are associated with earlier onset of acute breast skin reactions of BC patients treated with radiotherapy. in EACR 2023 Congress : Annual Congress of the European Association for Cancer Research : Abstracts
EACR - European Association for Cancer Research., P-0422-P-0422.
https://hdl.handle.net/21.15107/rcub_vinar_12237

Petrović N, Srbljak Ćuk I, Marjanovć Đorić D, Petrović I, Paunović P, Medić Milijić N. Higher VEGFA expression levels are associated with earlier onset of acute breast skin reactions of BC patients treated with radiotherapy. in EACR 2023 Congress : Annual Congress of the European Association for Cancer Research : Abstracts. 2023;:P-0422-P-0422.
https://hdl.handle.net/21.15107/rcub_vinar_12237 .

Petrović, Nina, Srbljak Ćuk, I., Marjanovć Đorić, D., Petrović, I., Paunović, P., Medić Milijić, Nataša, "Higher VEGFA expression levels are associated with earlier onset of acute breast skin reactions of BC patients treated with radiotherapy" in EACR 2023 Congress : Annual Congress of the European Association for Cancer Research : Abstracts (2023):P-0422-P-0422,
https://hdl.handle.net/21.15107/rcub_vinar_12237 .

TY  - CONF
AU  - Matić, I.
AU  - Petrović, Nina
AU  - Tadić, V.
AU  - Maksimović, S.
AU  - Stanojković, T.
AU  - Zizovic, I.
PY  - 2023
UR  - https://web.archive.org/web/20231218093802/https://nwm.covr.be/EACR2023abstracts/data/HtmlApp/main.html
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12255
AB  - Introduction  The plant species belonging to the large genus Helichrysum are a valuable source of bioactive compounds. The aim of this study was to investigate the anticancer properties of the two Helichrysum italicum extracts obtained from plant material by supercritical CO2 extraction. Material and Methods  The cytotoxicity of the extracts was examined against six human cancer cell lines: cervical adenocarcinoma HeLa, lung carcinoma A549, prostate adenocarcinoma PC-3, breast adenocarcinoma MCF-7, melanoma A375, and chronic myelogenous leukemia K562, as well as against two human normal cell lines: lung fibroblasts MRC-5 and keratinocytes HaCaT. Cell cycle analysis was performed by flow cytometry. Gene and microRNA expression levels were measured by RT-qPCR. Results and Discussions  The H. italicum extracts exerted concentration-dependent and selective cytotoxic effects on cancer cells. HeLa, A375, and K562 cells were the most sensitive to the cytotoxic activity of both extracts. The extracts showed lower intensities of cytotoxic activity against normal HaCaT cells when compared with intensities of activity against K562, A375, and HeLa cells. An increases in the percentages of cells within subG1 and S phases of the cell cycle were observed in HeLa cells incubated for 24 h with IC50 and 2IC50 concentrations of the extracts in comparison with control cells. A similar effect on the cell cycle phase distribution was seen in A375 cells after 24 h incubation. The G2/M phase arrest was also detected in A375 cells exposed to IC50 concentrations of the extracts. Both extracts induced apoptosis in HeLa cells through the activation of effector caspase-3. The extracts triggered apoptosis through the intrinsic pathway mediated by caspase-9 and the extrinsic pathway mediated by caspase-8. Each of the extracts decreased expression levels of MMP2 in HeLa cells, slightly increased levels of MMP9, and increased levels of VEGFA. Up-regulations of genes involved in cancer invasion and progression, MMP2, MMP9, and VEGFA, were observed in A375 cells after 24 h treatment with extracts. The H. italicum extracts increased the expression levels of miR-16, miR-21, and miR-155 in HeLa cells, and decreased the expression levels of these miRNAs in A375 cells, which could be explained by their cancer-specific roles and signatures. Conclusion  The results of this study point out promising cancer-suppressive properties of Helichrysum italicum extracts rich in bioactive phytochemicals.
PB  - EACR - European Association for Cancer Research
C3  - EACR 2023 Congress : Annual Congress of the European Association for Cancer Research : Abstracts
T1  - Anticancer potential of two Helichrysum italicum extracts obtained by supercritical CO2 extraction
SP  - P-0155
EP  - P-0155
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12255
ER  - 

@conference{
author = "Matić, I. and Petrović, Nina and Tadić, V. and Maksimović, S. and Stanojković, T. and Zizovic, I.",
year = "2023",
abstract = "Introduction  The plant species belonging to the large genus Helichrysum are a valuable source of bioactive compounds. The aim of this study was to investigate the anticancer properties of the two Helichrysum italicum extracts obtained from plant material by supercritical CO2 extraction. Material and Methods  The cytotoxicity of the extracts was examined against six human cancer cell lines: cervical adenocarcinoma HeLa, lung carcinoma A549, prostate adenocarcinoma PC-3, breast adenocarcinoma MCF-7, melanoma A375, and chronic myelogenous leukemia K562, as well as against two human normal cell lines: lung fibroblasts MRC-5 and keratinocytes HaCaT. Cell cycle analysis was performed by flow cytometry. Gene and microRNA expression levels were measured by RT-qPCR. Results and Discussions  The H. italicum extracts exerted concentration-dependent and selective cytotoxic effects on cancer cells. HeLa, A375, and K562 cells were the most sensitive to the cytotoxic activity of both extracts. The extracts showed lower intensities of cytotoxic activity against normal HaCaT cells when compared with intensities of activity against K562, A375, and HeLa cells. An increases in the percentages of cells within subG1 and S phases of the cell cycle were observed in HeLa cells incubated for 24 h with IC50 and 2IC50 concentrations of the extracts in comparison with control cells. A similar effect on the cell cycle phase distribution was seen in A375 cells after 24 h incubation. The G2/M phase arrest was also detected in A375 cells exposed to IC50 concentrations of the extracts. Both extracts induced apoptosis in HeLa cells through the activation of effector caspase-3. The extracts triggered apoptosis through the intrinsic pathway mediated by caspase-9 and the extrinsic pathway mediated by caspase-8. Each of the extracts decreased expression levels of MMP2 in HeLa cells, slightly increased levels of MMP9, and increased levels of VEGFA. Up-regulations of genes involved in cancer invasion and progression, MMP2, MMP9, and VEGFA, were observed in A375 cells after 24 h treatment with extracts. The H. italicum extracts increased the expression levels of miR-16, miR-21, and miR-155 in HeLa cells, and decreased the expression levels of these miRNAs in A375 cells, which could be explained by their cancer-specific roles and signatures. Conclusion  The results of this study point out promising cancer-suppressive properties of Helichrysum italicum extracts rich in bioactive phytochemicals.",
publisher = "EACR - European Association for Cancer Research",
journal = "EACR 2023 Congress : Annual Congress of the European Association for Cancer Research : Abstracts",
title = "Anticancer potential of two Helichrysum italicum extracts obtained by supercritical CO2 extraction",
pages = "P-0155-P-0155",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12255"
}

Matić, I., Petrović, N., Tadić, V., Maksimović, S., Stanojković, T.,& Zizovic, I.. (2023). Anticancer potential of two Helichrysum italicum extracts obtained by supercritical CO2 extraction. in EACR 2023 Congress : Annual Congress of the European Association for Cancer Research : Abstracts
EACR - European Association for Cancer Research., P-0155-P-0155.
https://hdl.handle.net/21.15107/rcub_vinar_12255

Matić I, Petrović N, Tadić V, Maksimović S, Stanojković T, Zizovic I. Anticancer potential of two Helichrysum italicum extracts obtained by supercritical CO2 extraction. in EACR 2023 Congress : Annual Congress of the European Association for Cancer Research : Abstracts. 2023;:P-0155-P-0155.
https://hdl.handle.net/21.15107/rcub_vinar_12255 .

Matić, I., Petrović, Nina, Tadić, V., Maksimović, S., Stanojković, T., Zizovic, I., "Anticancer potential of two Helichrysum italicum extracts obtained by supercritical CO2 extraction" in EACR 2023 Congress : Annual Congress of the European Association for Cancer Research : Abstracts (2023):P-0155-P-0155,
https://hdl.handle.net/21.15107/rcub_vinar_12255 .

TY  - CONF
AU  - Pašić, I.
AU  - Matić, I.
AU  - Petrović, Nina
AU  - Preljević, K.
AU  - Stanojković, T.
AU  - Perović, S.
PY  - 2023
UR  - https://web.archive.org/web/20231218093802/https://nwm.covr.be/EACR2023abstracts/data/HtmlApp/main.html
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12256
AB  - Introduction  The phytochemicals present in essential oils derived from aromatic plants of genus Thymus have been reported to exert antioxidant, anti-inflammatory, and anticancer effects. The aim of the research was to examine the cytotoxic activity and the mechanisms of anticancer action of the two essential oils obtained from Thymus vulgaris and Thymus serpyllum grown in Montenegro. Material and Methods  The cytotoxic activity was determined against four human cancer cell lines: cervical adenocarcinoma HeLa, malignant melanoma A375, colorectal adenocarcinoma LS 174T, and lung carcinoma A549, as well as against normal lung fibroblasts MRC-5 by MTT assay. The cell cycle phase distribution of HeLa cells and the potential activation of caspase-3, caspase-8, and caspase-9 were investigated by flow cytometry. Gene and microRNA expression levels in HeLa cells were measured using RT-qPCR. The intracellular levels of reactive oxygen species (ROS) in MRC-5 cells were measured by flow cytometry. Results and Discussions  Both essential oils exerted strong cytotoxicity against cancer cell lines with IC50 concentrations in the range from 0,20 to 0,24 µL/mL for T. vulgaris and from 0.32 to 0.49 µL/mL for T. serpyllum. Strong cytotoxicity was observed against lung fibroblasts MRC-5. The remarkable increases in the percentage of HeLa cells in the subG1 phase of the cell cycle after 24 h treatment with T. vulgaris and T. serpyllum essential oils were observed in comparison to the control cells. Pretreatment of HeLa cells with caspase inhibitors showed that T. vulgaris oil induced apoptotic cell death through caspase-3 and caspase-8, while T. serpyllum oil induced apoptosis through caspase-3 activation. Both essential oils decreased intracellular ROS levels in MRC-5 cells and reduced levels of oxidative stress induced by hydrogen peroxide. The treatment of HeLa cells with T. vulgaris oil lowered the MMP2 expression levels, increased MMP9 and VEGFA levels when compared with control cells, while T. serpyllum oil decreased the levels of MMP2 and MMP9, and increased VEGFA levels. HeLa cells treated with T. vulgaris oil had increased levels of miR-21, miR-16, and miR-34a, while cells treated with T. serpyllum oil had lower miR-16 and miR-34a levels, but higher miR-21 levels. Conclusion  These initial findings suggest that Thymus essential oils might have significant potential as cancer-chemopreventive and cancer-therapeutic agents, but further studies are necessary to evaluate their in vivo efficacy and safety.
PB  - EACR - European Association for Cancer Research
C3  - EACR 2023 Congress : Annual Congress of the European Association for Cancer Research : Abstracts
T1  - Anticancer effects of Thymus vulgaris and Thymus serpyllum essential oils from Montenegro
SP  - P-0111
EP  - P-0111
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12256
ER  - 

@conference{
author = "Pašić, I. and Matić, I. and Petrović, Nina and Preljević, K. and Stanojković, T. and Perović, S.",
year = "2023",
abstract = "Introduction  The phytochemicals present in essential oils derived from aromatic plants of genus Thymus have been reported to exert antioxidant, anti-inflammatory, and anticancer effects. The aim of the research was to examine the cytotoxic activity and the mechanisms of anticancer action of the two essential oils obtained from Thymus vulgaris and Thymus serpyllum grown in Montenegro. Material and Methods  The cytotoxic activity was determined against four human cancer cell lines: cervical adenocarcinoma HeLa, malignant melanoma A375, colorectal adenocarcinoma LS 174T, and lung carcinoma A549, as well as against normal lung fibroblasts MRC-5 by MTT assay. The cell cycle phase distribution of HeLa cells and the potential activation of caspase-3, caspase-8, and caspase-9 were investigated by flow cytometry. Gene and microRNA expression levels in HeLa cells were measured using RT-qPCR. The intracellular levels of reactive oxygen species (ROS) in MRC-5 cells were measured by flow cytometry. Results and Discussions  Both essential oils exerted strong cytotoxicity against cancer cell lines with IC50 concentrations in the range from 0,20 to 0,24 µL/mL for T. vulgaris and from 0.32 to 0.49 µL/mL for T. serpyllum. Strong cytotoxicity was observed against lung fibroblasts MRC-5. The remarkable increases in the percentage of HeLa cells in the subG1 phase of the cell cycle after 24 h treatment with T. vulgaris and T. serpyllum essential oils were observed in comparison to the control cells. Pretreatment of HeLa cells with caspase inhibitors showed that T. vulgaris oil induced apoptotic cell death through caspase-3 and caspase-8, while T. serpyllum oil induced apoptosis through caspase-3 activation. Both essential oils decreased intracellular ROS levels in MRC-5 cells and reduced levels of oxidative stress induced by hydrogen peroxide. The treatment of HeLa cells with T. vulgaris oil lowered the MMP2 expression levels, increased MMP9 and VEGFA levels when compared with control cells, while T. serpyllum oil decreased the levels of MMP2 and MMP9, and increased VEGFA levels. HeLa cells treated with T. vulgaris oil had increased levels of miR-21, miR-16, and miR-34a, while cells treated with T. serpyllum oil had lower miR-16 and miR-34a levels, but higher miR-21 levels. Conclusion  These initial findings suggest that Thymus essential oils might have significant potential as cancer-chemopreventive and cancer-therapeutic agents, but further studies are necessary to evaluate their in vivo efficacy and safety.",
publisher = "EACR - European Association for Cancer Research",
journal = "EACR 2023 Congress : Annual Congress of the European Association for Cancer Research : Abstracts",
title = "Anticancer effects of Thymus vulgaris and Thymus serpyllum essential oils from Montenegro",
pages = "P-0111-P-0111",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12256"
}

Pašić, I., Matić, I., Petrović, N., Preljević, K., Stanojković, T.,& Perović, S.. (2023). Anticancer effects of Thymus vulgaris and Thymus serpyllum essential oils from Montenegro. in EACR 2023 Congress : Annual Congress of the European Association for Cancer Research : Abstracts
EACR - European Association for Cancer Research., P-0111-P-0111.
https://hdl.handle.net/21.15107/rcub_vinar_12256

Pašić I, Matić I, Petrović N, Preljević K, Stanojković T, Perović S. Anticancer effects of Thymus vulgaris and Thymus serpyllum essential oils from Montenegro. in EACR 2023 Congress : Annual Congress of the European Association for Cancer Research : Abstracts. 2023;:P-0111-P-0111.
https://hdl.handle.net/21.15107/rcub_vinar_12256 .

Pašić, I., Matić, I., Petrović, Nina, Preljević, K., Stanojković, T., Perović, S., "Anticancer effects of Thymus vulgaris and Thymus serpyllum essential oils from Montenegro" in EACR 2023 Congress : Annual Congress of the European Association for Cancer Research : Abstracts (2023):P-0111-P-0111,
https://hdl.handle.net/21.15107/rcub_vinar_12256 .

TY  - CONF
AU  - Vuković, Miodrag
AU  - Filipović, Lidija
AU  - Popović, Milica
AU  - Petrović, Nina
AU  - Tanić, Miljana
AU  - Janković, Radmila
AU  - Korać, Aleksandra
AU  - Čavić, Milena
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12502
AB  - Introduction: The incidence of lung cancer (LC) in Serbia has increased over the last three decades. Up to 40% of patients with advanced non-small cell lung cancer (NSCLC) develop pleural effusion (PE). The aim of this study was to evaluate the usability of existing methods for isolation of extracellular vesicles from PE samples of patients with advanced non-small cell lung cancer and to characterize them for further use in the clinical/diagnostic/research setting. Material & Methods: PE samples diluted in PBS (1:1) from patients with advanced NSCLC were used. In-house spherical porous methacrylate-based copolymer coupled with VHH antibodies was used for the isolation of extracellular vesicles (EV) from PE samples. Flow-cytometry was performed for detection of exosomal markers. Results: A pool of PE was prepared from 5 patients with advanced NSCLC. Flow cytometry confirmed that the isolation of EVs was successful using the in-house affinity chromatography method. The presence of CD9 antigen was detected, as well as a decrease in the signal after the addition of Triton X-100. Further plans include the analysis of CD63 and CD81 antigens using flow cytometry, NTA analysis to determine the number and diameter of obtained vesicles, as well as TEM and SEM to determine their morphology. Discussion: We aim to investigate for the first time whether this method is applicable to pleural effusion samples as a new cancer liquid biopsy sample type. We also plan to evaluate the method in comparison with a commercial isolation kit.
PB  - Serbian Society for Extracellular Vesicles (SrbEVs)
PB  - Austrian Society for Extracellular Vesicles (ASEV)
PB  - Hungarian Society for Extracellular Vesicles (HSEV)
PB  - Slovenian Network for Extracellular Vesicles (SiN-EV)
C3  - Small New World 2.0 : Book of abstracts
T1  - Isolation and characterization of extracellular vesicles from pleural effusion samples of patients with advanced non-small cell lung cancer
SP  - 51
EP  - 51
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12502
ER  - 

@conference{
author = "Vuković, Miodrag and Filipović, Lidija and Popović, Milica and Petrović, Nina and Tanić, Miljana and Janković, Radmila and Korać, Aleksandra and Čavić, Milena",
year = "2023",
abstract = "Introduction: The incidence of lung cancer (LC) in Serbia has increased over the last three decades. Up to 40% of patients with advanced non-small cell lung cancer (NSCLC) develop pleural effusion (PE). The aim of this study was to evaluate the usability of existing methods for isolation of extracellular vesicles from PE samples of patients with advanced non-small cell lung cancer and to characterize them for further use in the clinical/diagnostic/research setting. Material & Methods: PE samples diluted in PBS (1:1) from patients with advanced NSCLC were used. In-house spherical porous methacrylate-based copolymer coupled with VHH antibodies was used for the isolation of extracellular vesicles (EV) from PE samples. Flow-cytometry was performed for detection of exosomal markers. Results: A pool of PE was prepared from 5 patients with advanced NSCLC. Flow cytometry confirmed that the isolation of EVs was successful using the in-house affinity chromatography method. The presence of CD9 antigen was detected, as well as a decrease in the signal after the addition of Triton X-100. Further plans include the analysis of CD63 and CD81 antigens using flow cytometry, NTA analysis to determine the number and diameter of obtained vesicles, as well as TEM and SEM to determine their morphology. Discussion: We aim to investigate for the first time whether this method is applicable to pleural effusion samples as a new cancer liquid biopsy sample type. We also plan to evaluate the method in comparison with a commercial isolation kit.",
publisher = "Serbian Society for Extracellular Vesicles (SrbEVs), Austrian Society for Extracellular Vesicles (ASEV), Hungarian Society for Extracellular Vesicles (HSEV), Slovenian Network for Extracellular Vesicles (SiN-EV)",
journal = "Small New World 2.0 : Book of abstracts",
title = "Isolation and characterization of extracellular vesicles from pleural effusion samples of patients with advanced non-small cell lung cancer",
pages = "51-51",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12502"
}

Vuković, M., Filipović, L., Popović, M., Petrović, N., Tanić, M., Janković, R., Korać, A.,& Čavić, M.. (2023). Isolation and characterization of extracellular vesicles from pleural effusion samples of patients with advanced non-small cell lung cancer. in Small New World 2.0 : Book of abstracts
Serbian Society for Extracellular Vesicles (SrbEVs)., 51-51.
https://hdl.handle.net/21.15107/rcub_vinar_12502

Vuković M, Filipović L, Popović M, Petrović N, Tanić M, Janković R, Korać A, Čavić M. Isolation and characterization of extracellular vesicles from pleural effusion samples of patients with advanced non-small cell lung cancer. in Small New World 2.0 : Book of abstracts. 2023;:51-51.
https://hdl.handle.net/21.15107/rcub_vinar_12502 .

Vuković, Miodrag, Filipović, Lidija, Popović, Milica, Petrović, Nina, Tanić, Miljana, Janković, Radmila, Korać, Aleksandra, Čavić, Milena, "Isolation and characterization of extracellular vesicles from pleural effusion samples of patients with advanced non-small cell lung cancer" in Small New World 2.0 : Book of abstracts (2023):51-51,
https://hdl.handle.net/21.15107/rcub_vinar_12502 .

TY  - JOUR
AU  - Mısır, Sema
AU  - Yaman, Serap Özer
AU  - Petrović, Nina
AU  - Sumer, Ceren
AU  - Hepokur, Ceylan
AU  - Aliyazicioglu, Yüksel
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10630
AB  - Breast cancer (BC) is the most common heterogeneous disease in women and one of the leading causes of cancer-related death. Surgery, chemotherapy, radiotherapy, hormone, and targeted therapy are the gold standards for BC treatment. One of the significant challenges during the treatment of BC represents resistance to chemotherapeutics, resistance that severely limits the use and effectiveness of the drugs used for BC treatment. Therefore, it is essential to develop new strategies to improve therapeutic efficacy. Circular RNAs (circRNAs) are a large group of non-coding RNAs that covalently form closed circular loops by joining their 5′, and 3′; ends. Accumulating evidence suggests that circRNAs have a vital role in cancer development, progression, and BC resistance to chemotherapy. The purpose of this review is to discuss the biological properties of circRNAs, and how circRNAs induce resistance to conventional therapeutic anti-cancer drugs used in BC treatment, by emphasizing and summarizing the potential roles of circRNAs in mechanisms of drug resistance, such as drug efflux, apoptosis dysfunction, autophagy, and DNA damage repair. CircRNAs are associated with drug resistance via ATP-binding cassette (ABC) efflux transporters, while some others by inhibition of cell apoptosis, thus leading to resistance to tamoxifen in BC cells. In contrast, others are involved in the promotion of BC cells chemoresistance by doxorubicin-induced autophagy. CircRNAs may have clinical significance in regulating or overcoming BC drug resistance and may give directions towards a novel approach to personalized BC treatment. CircRNAs may significantly contribute to the identification of new therapeutic targets for the prevention of BC chemoresistance. © 2022, Tech Science Press. All rights reserved.
T2  - Oncology Research
T1  - circRNAs in drug resistance of breast cancer
VL  - 30
IS  - 4
SP  - 157
EP  - 172
DO  - 10.32604/or.2022.027547
ER  - 

@article{
author = "Mısır, Sema and Yaman, Serap Özer and Petrović, Nina and Sumer, Ceren and Hepokur, Ceylan and Aliyazicioglu, Yüksel",
year = "2022",
abstract = "Breast cancer (BC) is the most common heterogeneous disease in women and one of the leading causes of cancer-related death. Surgery, chemotherapy, radiotherapy, hormone, and targeted therapy are the gold standards for BC treatment. One of the significant challenges during the treatment of BC represents resistance to chemotherapeutics, resistance that severely limits the use and effectiveness of the drugs used for BC treatment. Therefore, it is essential to develop new strategies to improve therapeutic efficacy. Circular RNAs (circRNAs) are a large group of non-coding RNAs that covalently form closed circular loops by joining their 5′, and 3′; ends. Accumulating evidence suggests that circRNAs have a vital role in cancer development, progression, and BC resistance to chemotherapy. The purpose of this review is to discuss the biological properties of circRNAs, and how circRNAs induce resistance to conventional therapeutic anti-cancer drugs used in BC treatment, by emphasizing and summarizing the potential roles of circRNAs in mechanisms of drug resistance, such as drug efflux, apoptosis dysfunction, autophagy, and DNA damage repair. CircRNAs are associated with drug resistance via ATP-binding cassette (ABC) efflux transporters, while some others by inhibition of cell apoptosis, thus leading to resistance to tamoxifen in BC cells. In contrast, others are involved in the promotion of BC cells chemoresistance by doxorubicin-induced autophagy. CircRNAs may have clinical significance in regulating or overcoming BC drug resistance and may give directions towards a novel approach to personalized BC treatment. CircRNAs may significantly contribute to the identification of new therapeutic targets for the prevention of BC chemoresistance. © 2022, Tech Science Press. All rights reserved.",
journal = "Oncology Research",
title = "circRNAs in drug resistance of breast cancer",
volume = "30",
number = "4",
pages = "157-172",
doi = "10.32604/or.2022.027547"
}

Mısır, S., Yaman, S. Ö., Petrović, N., Sumer, C., Hepokur, C.,& Aliyazicioglu, Y.. (2022). circRNAs in drug resistance of breast cancer. in Oncology Research, 30(4), 157-172.
https://doi.org/10.32604/or.2022.027547

Mısır S, Yaman SÖ, Petrović N, Sumer C, Hepokur C, Aliyazicioglu Y. circRNAs in drug resistance of breast cancer. in Oncology Research. 2022;30(4):157-172.
doi:10.32604/or.2022.027547 .

Mısır, Sema, Yaman, Serap Özer, Petrović, Nina, Sumer, Ceren, Hepokur, Ceylan, Aliyazicioglu, Yüksel, "circRNAs in drug resistance of breast cancer" in Oncology Research, 30, no. 4 (2022):157-172,
https://doi.org/10.32604/or.2022.027547 . .

TY  - JOUR
AU  - Kokanov, Nikola
AU  - Krajnović, Milena M.
AU  - Jovanović-Ćupić, Snežana P.
AU  - Kožik, Bojana
AU  - Petrović, Nina
AU  - Božović, Ana M.
AU  - Mandušić, Vesna
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10273
AB  - Prevention of chronic hepatitis C (CHC) and its complications is based on antiviral therapy and early detection of reliable molecular markers in persons under risk. We investigated whether the methylation status of RASSF1A and p16 genes, alone or in combination with host and viral factors, affects the response to therapy with pegylated interferon/ribavirin (PEG-IFN/RBV). Methylation-specific polymerase chain reaction (MSP) was used to determine the methylation status of the target promoter sequences of RASSF1A and p16 in circulating-free DNA from the peripheral blood of 49 patients with CHC genotype 1b. The methylation status of the examined genes did not affect the response to therapy. However, the simultaneous presence of either RASSF1A or p16 methylation and the CC genotype of IL28B was significantly related to a sustained virologic response (P=0.009 and P=0.032, respectively). After Bonferroni correction, only the result concerning the RASSF1A gene remained significant (P<0.0125). Methylation of RASSF1A was associated with the CC genotype of the IL28B gene (P=0.024) and a higher viral load (≥400 000 IU/mL, P=0.009). Our results suggest that combined analysis of RASSF1A gene methylation and IL28B rs12979860 polymorphism could potentially help in the prediction of therapy response in CHC genotype 1b patients.
T2  - Archives of Biological Sciences
T1  - RASSF1A and p16 promoter methylation and treatment response in chronic hepatitis C genotype 1b patients treated with pegylated interferon/ribavirin
VL  - 74
IS  - 1
SP  - 57
EP  - 66
DO  - 10.2298/ABS211208004K
ER  - 

@article{
author = "Kokanov, Nikola and Krajnović, Milena M. and Jovanović-Ćupić, Snežana P. and Kožik, Bojana and Petrović, Nina and Božović, Ana M. and Mandušić, Vesna",
year = "2022",
abstract = "Prevention of chronic hepatitis C (CHC) and its complications is based on antiviral therapy and early detection of reliable molecular markers in persons under risk. We investigated whether the methylation status of RASSF1A and p16 genes, alone or in combination with host and viral factors, affects the response to therapy with pegylated interferon/ribavirin (PEG-IFN/RBV). Methylation-specific polymerase chain reaction (MSP) was used to determine the methylation status of the target promoter sequences of RASSF1A and p16 in circulating-free DNA from the peripheral blood of 49 patients with CHC genotype 1b. The methylation status of the examined genes did not affect the response to therapy. However, the simultaneous presence of either RASSF1A or p16 methylation and the CC genotype of IL28B was significantly related to a sustained virologic response (P=0.009 and P=0.032, respectively). After Bonferroni correction, only the result concerning the RASSF1A gene remained significant (P<0.0125). Methylation of RASSF1A was associated with the CC genotype of the IL28B gene (P=0.024) and a higher viral load (≥400 000 IU/mL, P=0.009). Our results suggest that combined analysis of RASSF1A gene methylation and IL28B rs12979860 polymorphism could potentially help in the prediction of therapy response in CHC genotype 1b patients.",
journal = "Archives of Biological Sciences",
title = "RASSF1A and p16 promoter methylation and treatment response in chronic hepatitis C genotype 1b patients treated with pegylated interferon/ribavirin",
volume = "74",
number = "1",
pages = "57-66",
doi = "10.2298/ABS211208004K"
}

Kokanov, N., Krajnović, M. M., Jovanović-Ćupić, S. P., Kožik, B., Petrović, N., Božović, A. M.,& Mandušić, V.. (2022). RASSF1A and p16 promoter methylation and treatment response in chronic hepatitis C genotype 1b patients treated with pegylated interferon/ribavirin. in Archives of Biological Sciences, 74(1), 57-66.
https://doi.org/10.2298/ABS211208004K

Kokanov N, Krajnović MM, Jovanović-Ćupić SP, Kožik B, Petrović N, Božović AM, Mandušić V. RASSF1A and p16 promoter methylation and treatment response in chronic hepatitis C genotype 1b patients treated with pegylated interferon/ribavirin. in Archives of Biological Sciences. 2022;74(1):57-66.
doi:10.2298/ABS211208004K .

Kokanov, Nikola, Krajnović, Milena M., Jovanović-Ćupić, Snežana P., Kožik, Bojana, Petrović, Nina, Božović, Ana M., Mandušić, Vesna, "RASSF1A and p16 promoter methylation and treatment response in chronic hepatitis C genotype 1b patients treated with pegylated interferon/ribavirin" in Archives of Biological Sciences, 74, no. 1 (2022):57-66,
https://doi.org/10.2298/ABS211208004K . .

TY  - CONF
AU  - Todorović, Lidija
AU  - Petrović, Nina
AU  - Mandušić, Vesna
AU  - Živaljević, Vladan
AU  - Paunović, Ivan
AU  - Stanojević, Boban
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12426
AB  - Although one of the rarest known malignancies, with an estimated prevalence of 0.005% of all cancers, parathyroid carcinoma (PC) represents a clinical and therapeutic challenge. The identification of novel diagnostic biomarkers able to preoperatively distinguish among different parathyroid neoplastic types is of great clinical importance. However, there is a lack of both experimental and bioinformatics data in this field. In the present study, we examined the expression levels of two microRNAs frequently implicated in cancer, miR-30a-3p and miR-92a-3p, in 10 PC and 10 parathyroid adenoma (PA) tissues using reverse transcription quantitative (RT-qPCR). In addition, we analyzed their association with clinical and histopathological parameters. For statistical analysis we used non-parametric tests – Mann–Whitney U test and Spearman’s correlation test. Furthermore, by using several online tools such as miRNet, miRror Suit, and DisGeNET we analyzed combinatorial target genes of the two miRs and investigated their potential roles in parathyroid carcinoma and adenoma. The expression levels of both miR-30a-3p and miR-92a-3p were upregulated in PC compared to PA (p= 0.017 and p=0.0015 respectively). A positive correlation between their expression levels was identified both in PC (p=0.025, r=0.697) and PA (p=0.008, r=0745), indicating their combined action. There were no significant associations between the expression levels of these two miRs and clinicopathological characteristics in the PC group. According to the in silico analysis, the two miRs share a number of target genes, predominantly included in gene expression, cell cycle regulation, and several signalling pathways such as WNT/β catenin, which was found to be frequently aberrant in PC. Although their role and diagnostic utility remain to be elucidated, the data here reported suggest that miR-30a-3p and miR-92a-3p might be involved in parathyroid cancer pathogenesis and that they might be candidates for differential diagnosis between parathyroid carcinoma and adenoma.
C3  - 5th Annual Meeting STRATAGEM: New diagnostic and therapeutic tools against multidrug resistant tumours : Book of abstracts
T1  - miR-30a-3p and miR-92a-3p as potential diagnostic biomarkers in parathyroid carcinoma
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12426
ER  - 

@conference{
author = "Todorović, Lidija and Petrović, Nina and Mandušić, Vesna and Živaljević, Vladan and Paunović, Ivan and Stanojević, Boban",
year = "2022",
abstract = "Although one of the rarest known malignancies, with an estimated prevalence of 0.005% of all cancers, parathyroid carcinoma (PC) represents a clinical and therapeutic challenge. The identification of novel diagnostic biomarkers able to preoperatively distinguish among different parathyroid neoplastic types is of great clinical importance. However, there is a lack of both experimental and bioinformatics data in this field. In the present study, we examined the expression levels of two microRNAs frequently implicated in cancer, miR-30a-3p and miR-92a-3p, in 10 PC and 10 parathyroid adenoma (PA) tissues using reverse transcription quantitative (RT-qPCR). In addition, we analyzed their association with clinical and histopathological parameters. For statistical analysis we used non-parametric tests – Mann–Whitney U test and Spearman’s correlation test. Furthermore, by using several online tools such as miRNet, miRror Suit, and DisGeNET we analyzed combinatorial target genes of the two miRs and investigated their potential roles in parathyroid carcinoma and adenoma. The expression levels of both miR-30a-3p and miR-92a-3p were upregulated in PC compared to PA (p= 0.017 and p=0.0015 respectively). A positive correlation between their expression levels was identified both in PC (p=0.025, r=0.697) and PA (p=0.008, r=0745), indicating their combined action. There were no significant associations between the expression levels of these two miRs and clinicopathological characteristics in the PC group. According to the in silico analysis, the two miRs share a number of target genes, predominantly included in gene expression, cell cycle regulation, and several signalling pathways such as WNT/β catenin, which was found to be frequently aberrant in PC. Although their role and diagnostic utility remain to be elucidated, the data here reported suggest that miR-30a-3p and miR-92a-3p might be involved in parathyroid cancer pathogenesis and that they might be candidates for differential diagnosis between parathyroid carcinoma and adenoma.",
journal = "5th Annual Meeting STRATAGEM: New diagnostic and therapeutic tools against multidrug resistant tumours : Book of abstracts",
title = "miR-30a-3p and miR-92a-3p as potential diagnostic biomarkers in parathyroid carcinoma",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12426"
}

Todorović, L., Petrović, N., Mandušić, V., Živaljević, V., Paunović, I.,& Stanojević, B.. (2022). miR-30a-3p and miR-92a-3p as potential diagnostic biomarkers in parathyroid carcinoma. in 5th Annual Meeting STRATAGEM: New diagnostic and therapeutic tools against multidrug resistant tumours : Book of abstracts.
https://hdl.handle.net/21.15107/rcub_vinar_12426

Todorović L, Petrović N, Mandušić V, Živaljević V, Paunović I, Stanojević B. miR-30a-3p and miR-92a-3p as potential diagnostic biomarkers in parathyroid carcinoma. in 5th Annual Meeting STRATAGEM: New diagnostic and therapeutic tools against multidrug resistant tumours : Book of abstracts. 2022;.
https://hdl.handle.net/21.15107/rcub_vinar_12426 .

Todorović, Lidija, Petrović, Nina, Mandušić, Vesna, Živaljević, Vladan, Paunović, Ivan, Stanojević, Boban, "miR-30a-3p and miR-92a-3p as potential diagnostic biomarkers in parathyroid carcinoma" in 5th Annual Meeting STRATAGEM: New diagnostic and therapeutic tools against multidrug resistant tumours : Book of abstracts (2022),
https://hdl.handle.net/21.15107/rcub_vinar_12426 .

TY  - JOUR
AU  - Stepanović, Aleksandar
AU  - Nikitović, Marina
AU  - Stanojković, Tatjana P.
AU  - Grujičić, Danica
AU  - Bukumirić, Zoran
AU  - Srbljak, Ivana
AU  - Ilić, Rosanda
AU  - Milošević, Snežana
AU  - Arsenijević, Tatjana
AU  - Petrović, Nina
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10265
AB  - A personalized approach to chemoradiation is important in reducing its potential side effects and identifying a group of patients prone to toxicity. MicroRNAs have been shown to have a predictive potential for radiotoxicity. The goal of the study was to test if levels of miRNA in peripheral blood mononuclear cells of glioblastoma patients are associated with toxicity and to identify the peak time point for toxicity. MicroRNA-10b/21/34a levels were measured in 43 patients with and without toxicity, at baseline, at the 15th, and at the 30th fraction by Real-Time quantitative Polymerase Chain Reaction. MicroRNA-10b/21 levels increased with toxicity grade (p = 0.014; p = 0.013); miR-21/34a levels were significantly different between patients with and without toxicity at the 15th fraction (p = 0.030; p = 0.045), while miR-34a levels significantly changed during treatment (p < 0.001). All three miRNAs showed a significantly high positive correlation with one another. MiR-34a might be considered as a predictive factor for toxicity due to its changes during treatment, and differences between the groups with and without toxicity; miR-10b might be used to predict toxicity; miR-10b/21 might be used for predicting the grade of toxicity in GB patients.
T2  - Scientific Reports
T1  - Association between microRNAs 10b/21/34a and acute toxicity in glioblastoma patients treated with radiotherapy and temozolomide
VL  - 12
IS  - 1
SP  - 7505
DO  - 10.1038/s41598-022-11445-9
ER  - 

@article{
author = "Stepanović, Aleksandar and Nikitović, Marina and Stanojković, Tatjana P. and Grujičić, Danica and Bukumirić, Zoran and Srbljak, Ivana and Ilić, Rosanda and Milošević, Snežana and Arsenijević, Tatjana and Petrović, Nina",
year = "2022",
abstract = "A personalized approach to chemoradiation is important in reducing its potential side effects and identifying a group of patients prone to toxicity. MicroRNAs have been shown to have a predictive potential for radiotoxicity. The goal of the study was to test if levels of miRNA in peripheral blood mononuclear cells of glioblastoma patients are associated with toxicity and to identify the peak time point for toxicity. MicroRNA-10b/21/34a levels were measured in 43 patients with and without toxicity, at baseline, at the 15th, and at the 30th fraction by Real-Time quantitative Polymerase Chain Reaction. MicroRNA-10b/21 levels increased with toxicity grade (p = 0.014; p = 0.013); miR-21/34a levels were significantly different between patients with and without toxicity at the 15th fraction (p = 0.030; p = 0.045), while miR-34a levels significantly changed during treatment (p < 0.001). All three miRNAs showed a significantly high positive correlation with one another. MiR-34a might be considered as a predictive factor for toxicity due to its changes during treatment, and differences between the groups with and without toxicity; miR-10b might be used to predict toxicity; miR-10b/21 might be used for predicting the grade of toxicity in GB patients.",
journal = "Scientific Reports",
title = "Association between microRNAs 10b/21/34a and acute toxicity in glioblastoma patients treated with radiotherapy and temozolomide",
volume = "12",
number = "1",
pages = "7505",
doi = "10.1038/s41598-022-11445-9"
}

Stepanović, A., Nikitović, M., Stanojković, T. P., Grujičić, D., Bukumirić, Z., Srbljak, I., Ilić, R., Milošević, S., Arsenijević, T.,& Petrović, N.. (2022). Association between microRNAs 10b/21/34a and acute toxicity in glioblastoma patients treated with radiotherapy and temozolomide. in Scientific Reports, 12(1), 7505.
https://doi.org/10.1038/s41598-022-11445-9

Stepanović A, Nikitović M, Stanojković TP, Grujičić D, Bukumirić Z, Srbljak I, Ilić R, Milošević S, Arsenijević T, Petrović N. Association between microRNAs 10b/21/34a and acute toxicity in glioblastoma patients treated with radiotherapy and temozolomide. in Scientific Reports. 2022;12(1):7505.
doi:10.1038/s41598-022-11445-9 .

Stepanović, Aleksandar, Nikitović, Marina, Stanojković, Tatjana P., Grujičić, Danica, Bukumirić, Zoran, Srbljak, Ivana, Ilić, Rosanda, Milošević, Snežana, Arsenijević, Tatjana, Petrović, Nina, "Association between microRNAs 10b/21/34a and acute toxicity in glioblastoma patients treated with radiotherapy and temozolomide" in Scientific Reports, 12, no. 1 (2022):7505,
https://doi.org/10.1038/s41598-022-11445-9 . .

TY  - JOUR
AU  - Roganović, Jelena
AU  - Petrović, Nina
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10401
AB  - Non-coding RNAs (ncRNAs) represent a research hotspot by playing a key role in epigenetic and transcriptional regulation of diverse biological functions and due to their involvement in different diseases, including oral inflammatory diseases. Based on ncRNAs’ suitability for salivary biomarkers and their involvement in neuropathic pain and tissue regeneration signaling pathways, the present narrative review aims to highlight the potential clinical applications of ncRNAs in oral inflammatory diseases, with an emphasis on salivary diagnostics, regenerative dentistry, and precision medicine for neuropathic orofacial pain.
T2  - International Journal of Molecular Sciences
T1  - Clinical Perspectives of Non-Coding RNA in Oral Inflammatory Diseases and Neuropathic Pain: A Narrative Review
VL  - 23
IS  - 15
SP  - 8278
DO  - 10.3390/ijms23158278
ER  - 

@article{
author = "Roganović, Jelena and Petrović, Nina",
year = "2022",
abstract = "Non-coding RNAs (ncRNAs) represent a research hotspot by playing a key role in epigenetic and transcriptional regulation of diverse biological functions and due to their involvement in different diseases, including oral inflammatory diseases. Based on ncRNAs’ suitability for salivary biomarkers and their involvement in neuropathic pain and tissue regeneration signaling pathways, the present narrative review aims to highlight the potential clinical applications of ncRNAs in oral inflammatory diseases, with an emphasis on salivary diagnostics, regenerative dentistry, and precision medicine for neuropathic orofacial pain.",
journal = "International Journal of Molecular Sciences",
title = "Clinical Perspectives of Non-Coding RNA in Oral Inflammatory Diseases and Neuropathic Pain: A Narrative Review",
volume = "23",
number = "15",
pages = "8278",
doi = "10.3390/ijms23158278"
}

Roganović, J.,& Petrović, N.. (2022). Clinical Perspectives of Non-Coding RNA in Oral Inflammatory Diseases and Neuropathic Pain: A Narrative Review. in International Journal of Molecular Sciences, 23(15), 8278.
https://doi.org/10.3390/ijms23158278

Roganović J, Petrović N. Clinical Perspectives of Non-Coding RNA in Oral Inflammatory Diseases and Neuropathic Pain: A Narrative Review. in International Journal of Molecular Sciences. 2022;23(15):8278.
doi:10.3390/ijms23158278 .

Roganović, Jelena, Petrović, Nina, "Clinical Perspectives of Non-Coding RNA in Oral Inflammatory Diseases and Neuropathic Pain: A Narrative Review" in International Journal of Molecular Sciences, 23, no. 15 (2022):8278,
https://doi.org/10.3390/ijms23158278 . .

TY  - JOUR
AU  - Mališić, Emina
AU  - Petrović, Nina
AU  - Brengues, Muriel
AU  - Azria, David
AU  - Matić, Ivana Z.
AU  - Srbljak Ćuk, Ivana
AU  - Kopčalić, Katarina
AU  - Stanojković, Tatjana P.
AU  - Nikitović, Marina
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10544
AB  - The genetic background of each person might affect the severity of radiotherapy (RT)-induced normal tissue toxicity. The aim of study was to evaluate the influence of TGFB1 C-509T and Leu10Pro, XRCC1 Arg280His and XRCC3 Thr241Met polymorphisms as well as the level of radiation-induced CD8 T-lymphocyte apoptosis (RILA) on adverse effects of RT for prostate cancer (PCa). The study included 88 patients with localized or locally advanced PCa who were treated with RT. The polymorphisms were determined by PCR–RFLP analysis on DNA from peripheral blood mononuclear cells. RILA values were measured by flow cytometry. We found that CT genotype of TGFB1 C-509T could be protective biomarker for acute genitourinary (GU) and gastrointestinal (GI) radiotoxicity, while Thr variant of XRCC3 Thr241Met could predict the risk for acute GU radiotoxicity. Correlation between RILA values and toxicity was not detected. Univariate logistic regression analysis showed that Gleason score and risk group were risk factors for late GU, while for late GI radiotoxicity it was diabetes mellitus type 2. However, in multivariate model those were not proven to be significant and independent risk factors. Identification of assays combination predicting individual radiosensitivity is a crucial step towards personalized RT approach.
T2  - Scientific Reports
T1  - Association of polymorphisms in TGFB1, XRCC1, XRCC3 genes and CD8 T-lymphocyte apoptosis with adverse effect of radiotherapy for prostate cancer
VL  - 12
IS  - 1
SP  - 21306
DO  - 10.1038/s41598-022-25328-6
ER  - 

@article{
author = "Mališić, Emina and Petrović, Nina and Brengues, Muriel and Azria, David and Matić, Ivana Z. and Srbljak Ćuk, Ivana and Kopčalić, Katarina and Stanojković, Tatjana P. and Nikitović, Marina",
year = "2022",
abstract = "The genetic background of each person might affect the severity of radiotherapy (RT)-induced normal tissue toxicity. The aim of study was to evaluate the influence of TGFB1 C-509T and Leu10Pro, XRCC1 Arg280His and XRCC3 Thr241Met polymorphisms as well as the level of radiation-induced CD8 T-lymphocyte apoptosis (RILA) on adverse effects of RT for prostate cancer (PCa). The study included 88 patients with localized or locally advanced PCa who were treated with RT. The polymorphisms were determined by PCR–RFLP analysis on DNA from peripheral blood mononuclear cells. RILA values were measured by flow cytometry. We found that CT genotype of TGFB1 C-509T could be protective biomarker for acute genitourinary (GU) and gastrointestinal (GI) radiotoxicity, while Thr variant of XRCC3 Thr241Met could predict the risk for acute GU radiotoxicity. Correlation between RILA values and toxicity was not detected. Univariate logistic regression analysis showed that Gleason score and risk group were risk factors for late GU, while for late GI radiotoxicity it was diabetes mellitus type 2. However, in multivariate model those were not proven to be significant and independent risk factors. Identification of assays combination predicting individual radiosensitivity is a crucial step towards personalized RT approach.",
journal = "Scientific Reports",
title = "Association of polymorphisms in TGFB1, XRCC1, XRCC3 genes and CD8 T-lymphocyte apoptosis with adverse effect of radiotherapy for prostate cancer",
volume = "12",
number = "1",
pages = "21306",
doi = "10.1038/s41598-022-25328-6"
}

Mališić, E., Petrović, N., Brengues, M., Azria, D., Matić, I. Z., Srbljak Ćuk, I., Kopčalić, K., Stanojković, T. P.,& Nikitović, M.. (2022). Association of polymorphisms in TGFB1, XRCC1, XRCC3 genes and CD8 T-lymphocyte apoptosis with adverse effect of radiotherapy for prostate cancer. in Scientific Reports, 12(1), 21306.
https://doi.org/10.1038/s41598-022-25328-6

Mališić E, Petrović N, Brengues M, Azria D, Matić IZ, Srbljak Ćuk I, Kopčalić K, Stanojković TP, Nikitović M. Association of polymorphisms in TGFB1, XRCC1, XRCC3 genes and CD8 T-lymphocyte apoptosis with adverse effect of radiotherapy for prostate cancer. in Scientific Reports. 2022;12(1):21306.
doi:10.1038/s41598-022-25328-6 .

Mališić, Emina, Petrović, Nina, Brengues, Muriel, Azria, David, Matić, Ivana Z., Srbljak Ćuk, Ivana, Kopčalić, Katarina, Stanojković, Tatjana P., Nikitović, Marina, "Association of polymorphisms in TGFB1, XRCC1, XRCC3 genes and CD8 T-lymphocyte apoptosis with adverse effect of radiotherapy for prostate cancer" in Scientific Reports, 12, no. 1 (2022):21306,
https://doi.org/10.1038/s41598-022-25328-6 . .

TY  - JOUR
AU  - Petrović, Nina
AU  - Ergün, Sercan
AU  - Đorđić-Crnogorac, Marija
AU  - Stanojković, Tatjana
AU  - Mališić, Emina
AU  - Matić Ivana Z.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10706
AB  - Phytochemicals and bioactive substances derived from a wide range of plant extracts have been reported to exert various anticancer effects. Prostate cancer is one of the leading causes of cancer-related deaths within the male population. Prostate cancer-specific miRNA signatures were associated with cancer formation and progression, with various subtypes, and response to therapy. MicroRNA levels of expression were shown to change after the treatment of various compounds and substances extracted from natural products. Natural herbal compounds were shown to induce variations in miRNA expression levels in cancer cells. The aims of this study were to investigate the cytotoxic effects of methanol, ethyl-acetate, and hexane extracts obtained from branch-body part and flowers of Hypericum perforatum L. against humane PC-3 and DU 145 and to test potential miRNA-128/133b/155/193a/206/21/335 signature changes and differences between the two prostate cancer cell lines. Cytotoxic activity of H. perforatum extracts, their effects on cell cycle distribution, and miRNA expression levels were examined in humane PC-3 and DU 145 prostate cancer cells by MTT cell survival assay, flow cytometry, and quantitative real-time PCR. Hexane extract of flowers showed the strongest intensity of cytotoxic activity against PC-3 and DU 145 cells. The highest increase in the percentage of PC-3 cells in the subG1 phase was observed in cell samples treated with hexane extract of flowers and branch-body part. Significant differences in miRNA-128/133b/155/193a/206/21/335 levels were observed between PC-3 and DU 145 cell lines, especially in samples treated with flower extracts compared with the branch-body part. Conclusions: Investigated extracts have significant anticancer potential not only from the aspects of cytotoxicity and cell cycle effects but also from the aspect of lowering oncogenic or increasing tumor-suppressive miRNAs. The best effect might be the increase of tumor-suppressive miR-128 (accompanied by miR-193a) induced by the hexane extract of the flowers, which also exerted the highest cytotoxic activity. Hexane extract of flowers may be the candidate for further investigation for improving the efficiency of standard therapies for PCa. A miRNA signature might be cell-type specific after the treatment with H. perforatum extracts.
T2  - Genetika
T1  - Hypericum perforatum L. extracts exert cytotoxic effects and show different miRNA signatures in PC-3 and DU 145 prostate cancer cells
VL  - 54
IS  - 3
SP  - 1249
EP  - 1270
DO  - 10.2298/GENSR2203249P
ER  - 

@article{
author = "Petrović, Nina and Ergün, Sercan and Đorđić-Crnogorac, Marija and Stanojković, Tatjana and Mališić, Emina and Matić Ivana Z.",
year = "2022",
abstract = "Phytochemicals and bioactive substances derived from a wide range of plant extracts have been reported to exert various anticancer effects. Prostate cancer is one of the leading causes of cancer-related deaths within the male population. Prostate cancer-specific miRNA signatures were associated with cancer formation and progression, with various subtypes, and response to therapy. MicroRNA levels of expression were shown to change after the treatment of various compounds and substances extracted from natural products. Natural herbal compounds were shown to induce variations in miRNA expression levels in cancer cells. The aims of this study were to investigate the cytotoxic effects of methanol, ethyl-acetate, and hexane extracts obtained from branch-body part and flowers of Hypericum perforatum L. against humane PC-3 and DU 145 and to test potential miRNA-128/133b/155/193a/206/21/335 signature changes and differences between the two prostate cancer cell lines. Cytotoxic activity of H. perforatum extracts, their effects on cell cycle distribution, and miRNA expression levels were examined in humane PC-3 and DU 145 prostate cancer cells by MTT cell survival assay, flow cytometry, and quantitative real-time PCR. Hexane extract of flowers showed the strongest intensity of cytotoxic activity against PC-3 and DU 145 cells. The highest increase in the percentage of PC-3 cells in the subG1 phase was observed in cell samples treated with hexane extract of flowers and branch-body part. Significant differences in miRNA-128/133b/155/193a/206/21/335 levels were observed between PC-3 and DU 145 cell lines, especially in samples treated with flower extracts compared with the branch-body part. Conclusions: Investigated extracts have significant anticancer potential not only from the aspects of cytotoxicity and cell cycle effects but also from the aspect of lowering oncogenic or increasing tumor-suppressive miRNAs. The best effect might be the increase of tumor-suppressive miR-128 (accompanied by miR-193a) induced by the hexane extract of the flowers, which also exerted the highest cytotoxic activity. Hexane extract of flowers may be the candidate for further investigation for improving the efficiency of standard therapies for PCa. A miRNA signature might be cell-type specific after the treatment with H. perforatum extracts.",
journal = "Genetika",
title = "Hypericum perforatum L. extracts exert cytotoxic effects and show different miRNA signatures in PC-3 and DU 145 prostate cancer cells",
volume = "54",
number = "3",
pages = "1249-1270",
doi = "10.2298/GENSR2203249P"
}

Petrović, N., Ergün, S., Đorđić-Crnogorac, M., Stanojković, T., Mališić, E.,& Matić Ivana Z.. (2022). Hypericum perforatum L. extracts exert cytotoxic effects and show different miRNA signatures in PC-3 and DU 145 prostate cancer cells. in Genetika, 54(3), 1249-1270.
https://doi.org/10.2298/GENSR2203249P

Petrović N, Ergün S, Đorđić-Crnogorac M, Stanojković T, Mališić E, Matić Ivana Z.. Hypericum perforatum L. extracts exert cytotoxic effects and show different miRNA signatures in PC-3 and DU 145 prostate cancer cells. in Genetika. 2022;54(3):1249-1270.
doi:10.2298/GENSR2203249P .

Petrović, Nina, Ergün, Sercan, Đorđić-Crnogorac, Marija, Stanojković, Tatjana, Mališić, Emina, Matić Ivana Z., "Hypericum perforatum L. extracts exert cytotoxic effects and show different miRNA signatures in PC-3 and DU 145 prostate cancer cells" in Genetika, 54, no. 3 (2022):1249-1270,
https://doi.org/10.2298/GENSR2203249P . .

TY  - CONF
AU  - Pašić, Ivana
AU  - Srbljak Ćuk, Ivana
AU  - Petrović, Nina
AU  - Matić, Ivana
AU  - Džudžević-Čančar, Hurija
AU  - Dedić, Alema
AU  - Alispahić, Amra
AU  - Boškailo, Emina
AU  - Stanojković, Tatjana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12300
AB  - Lavandula angustifolia Mill. (lavender) is an aromatic and medicinal herb whose flower essential oils (EO) are widely used for the treatment of gastrointestinal, nervous, and rheumatic disorders, and in the perfume industry. Laurus nobilis L. (laurel bay) is an evergreen tree whose EOs have antimicrobial and anti-inflammatory effects. Lavender and bay were collected from Sarajevo and Mostar in Bosnia and Herzegovina. The extraction was performed by hydrodistillation in Clevenger-type apparatus. Phytochemical composition was analyzed by gas chromatography coupled with mass spectrometry. Cytotoxic activities of lavender EO and bay leaf, fruit and seed EOs were investigated against human cervical adenocarcinoma HeLa cells and non-transformed human lung fibroblasts MRC-5 by MTT cell survival assay. Cell cycle phase distribution was examined by flow cytometry. In bay EOs the most abundant component was 1,8-cineole, followed by linalool, bicyclic monoterpenes sabinene, αpinene, and β-pinene. Components identified in the fruit and seed, but not in the leaf were (E)-β-ocymene, camphene, β-elemene, bornyl acetate and trans-caryophyllene. The major component of lavender extract was linalool accompanied by linalyl acetate, lavandulyl acetate, camphor, 1,8-cneole, borneol, α-terpineol, and terpinene-4-ol. The four tested EOs showed concentration-dependent cytotoxic effects on HeLa and MRC-5 cells. Among examined EOs, lavender EO exerted the strongest cytotoxic activity on HeLa cells with IC50 value of 0.11 µL/mL. Bay seed and fruit EOs exerted stronger cytotoxicity on HeLa cells than bay leaf EO (IC50 values: 0.17, 0.21, and 3.35 µL/mL, respectively). When compared with sensitivity of HeLa cells, normal MRC-5 cells showed similar sensitivity to the cytotoxic activity of the four tested EOs. Lavender EO applied at IC50 concentration, during 24 h caused remarkable increase in the percentage of HeLa cells within the subG1 cell cycle phase, in comparison with control cells (64.69% vs 2.47%). Pretreatment with caspase-3, caspase-8 or caspase-9 inhibitor before 24 h treatment with lavender EO did not cause changes in the percentage of cells in the subG1 phase in comparison with HeLa cells exposed only to lavender oil. Our results showed that lavender and bay EOs exerted potent cytotoxic activity against HeLa cells. Additional investigations are necessary to explore cytotoxic effects of these EOs against various cancer cell lines and mechanisms underlying anticancer effects.
PB  - Zagreb : Croatian Association for Cancer Research
C3  - 6th Meeting of the Croatian Association for Cancer Research “HDIR-6: Targeting Cancer” : Book of abstracts
T1  - Cytotoxic Effects of Lavandula angustifolia Mill. and Laurus nobilis L. Essential Oils on Human Cervical Adenocarcinoma Cells
SP  - 43
EP  - 43
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12300
ER  - 

@conference{
author = "Pašić, Ivana and Srbljak Ćuk, Ivana and Petrović, Nina and Matić, Ivana and Džudžević-Čančar, Hurija and Dedić, Alema and Alispahić, Amra and Boškailo, Emina and Stanojković, Tatjana",
year = "2022",
abstract = "Lavandula angustifolia Mill. (lavender) is an aromatic and medicinal herb whose flower essential oils (EO) are widely used for the treatment of gastrointestinal, nervous, and rheumatic disorders, and in the perfume industry. Laurus nobilis L. (laurel bay) is an evergreen tree whose EOs have antimicrobial and anti-inflammatory effects. Lavender and bay were collected from Sarajevo and Mostar in Bosnia and Herzegovina. The extraction was performed by hydrodistillation in Clevenger-type apparatus. Phytochemical composition was analyzed by gas chromatography coupled with mass spectrometry. Cytotoxic activities of lavender EO and bay leaf, fruit and seed EOs were investigated against human cervical adenocarcinoma HeLa cells and non-transformed human lung fibroblasts MRC-5 by MTT cell survival assay. Cell cycle phase distribution was examined by flow cytometry. In bay EOs the most abundant component was 1,8-cineole, followed by linalool, bicyclic monoterpenes sabinene, αpinene, and β-pinene. Components identified in the fruit and seed, but not in the leaf were (E)-β-ocymene, camphene, β-elemene, bornyl acetate and trans-caryophyllene. The major component of lavender extract was linalool accompanied by linalyl acetate, lavandulyl acetate, camphor, 1,8-cneole, borneol, α-terpineol, and terpinene-4-ol. The four tested EOs showed concentration-dependent cytotoxic effects on HeLa and MRC-5 cells. Among examined EOs, lavender EO exerted the strongest cytotoxic activity on HeLa cells with IC50 value of 0.11 µL/mL. Bay seed and fruit EOs exerted stronger cytotoxicity on HeLa cells than bay leaf EO (IC50 values: 0.17, 0.21, and 3.35 µL/mL, respectively). When compared with sensitivity of HeLa cells, normal MRC-5 cells showed similar sensitivity to the cytotoxic activity of the four tested EOs. Lavender EO applied at IC50 concentration, during 24 h caused remarkable increase in the percentage of HeLa cells within the subG1 cell cycle phase, in comparison with control cells (64.69% vs 2.47%). Pretreatment with caspase-3, caspase-8 or caspase-9 inhibitor before 24 h treatment with lavender EO did not cause changes in the percentage of cells in the subG1 phase in comparison with HeLa cells exposed only to lavender oil. Our results showed that lavender and bay EOs exerted potent cytotoxic activity against HeLa cells. Additional investigations are necessary to explore cytotoxic effects of these EOs against various cancer cell lines and mechanisms underlying anticancer effects.",
publisher = "Zagreb : Croatian Association for Cancer Research",
journal = "6th Meeting of the Croatian Association for Cancer Research “HDIR-6: Targeting Cancer” : Book of abstracts",
title = "Cytotoxic Effects of Lavandula angustifolia Mill. and Laurus nobilis L. Essential Oils on Human Cervical Adenocarcinoma Cells",
pages = "43-43",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12300"
}

Pašić, I., Srbljak Ćuk, I., Petrović, N., Matić, I., Džudžević-Čančar, H., Dedić, A., Alispahić, A., Boškailo, E.,& Stanojković, T.. (2022). Cytotoxic Effects of Lavandula angustifolia Mill. and Laurus nobilis L. Essential Oils on Human Cervical Adenocarcinoma Cells. in 6th Meeting of the Croatian Association for Cancer Research “HDIR-6: Targeting Cancer” : Book of abstracts
Zagreb : Croatian Association for Cancer Research., 43-43.
https://hdl.handle.net/21.15107/rcub_vinar_12300

Pašić I, Srbljak Ćuk I, Petrović N, Matić I, Džudžević-Čančar H, Dedić A, Alispahić A, Boškailo E, Stanojković T. Cytotoxic Effects of Lavandula angustifolia Mill. and Laurus nobilis L. Essential Oils on Human Cervical Adenocarcinoma Cells. in 6th Meeting of the Croatian Association for Cancer Research “HDIR-6: Targeting Cancer” : Book of abstracts. 2022;:43-43.
https://hdl.handle.net/21.15107/rcub_vinar_12300 .

Pašić, Ivana, Srbljak Ćuk, Ivana, Petrović, Nina, Matić, Ivana, Džudžević-Čančar, Hurija, Dedić, Alema, Alispahić, Amra, Boškailo, Emina, Stanojković, Tatjana, "Cytotoxic Effects of Lavandula angustifolia Mill. and Laurus nobilis L. Essential Oils on Human Cervical Adenocarcinoma Cells" in 6th Meeting of the Croatian Association for Cancer Research “HDIR-6: Targeting Cancer” : Book of abstracts (2022):43-43,
https://hdl.handle.net/21.15107/rcub_vinar_12300 .

TY  - CONF
AU  - Mališić, Emina
AU  - Petrović, Nina
AU  - Nikitović, Marina
PY  - 2022
PY  - S1
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12422
AB  - Purpose or Objective About half of all prostate cancer (PCa) patients receive radiotherapy (RT) either as single curative treatment or as adjuvant/salvage treatment after radical prostatectomy. However, RT is associated with a spectrum of side effects (toxicity) in the surrounding normal tissues. Acute toxicity occurs within 90 days of treatment, is usually transient and affects skin and mucosa of bladder and intestine resulting in dermatitis, cystitis or diarrhea. XRCC3 gene encodes for protein that is involved in homologous recombination repair of double-strand breaks created by ionizing radiation. Disruption of these pathways has the potential to affect the normal tissue response to RT. The T variant of XRCC3 Thr241Met single nucleotide polymorphisms (SNP) in exon 7 (C>T, rs861539) was reported to be associated with elevated levels of DNA adducts, chromosomal deletions, sensitivity to ionizing radiation and cross-linking agents. The aim of this study was to examine association between this SNP and RT-induced normal tissue toxicity in PCa patients. Materials and Methods Eighty one patients who had a histologically confirmed localized or locally advanced PCa were included in the study. Patients were treated with 3DC RT (n=70) or ARC RT (n=11) with radical (72 Gy)(47 patients) or postoperative/salvage (66 Gy)(34 patients) RT without previous hormonal therapy. DNA from peripheral blood mononuclear cells was extracted by salting out method. XRCC3 Thr241Met SNP was determined by PCR-RFLP analysis. The restriction fragments were separated on 2100 Bioanalyzer using DNA 1000 kit. The differences in the distribution of genotypes of XRCC3 Thr241Met between patients with or without acute RT-induced genitourinary (GU) or gastrointestinal (GI) toxicity were tested by χ2 and Fisher’s exact test. P values ≤ 0.05 were considered statistically significant, while p values between 0.1 and 0.05 were pointed out as a statistical trend. Data were analyzed by SigmaStat 3.5. Results The acute GI toxicity appeared in 100%, 94.6% and 81.8% of ThrThr, ThrMet and MetMet PCa patients, raspectivelly. There was the statistical trend towards higher acute GU toxicity in carriers of Thr variant (ThrThr plus ThrMet) vs. MetMet (p=0.087) as well as ThrThr vs. MetMet (p=0.058). For acute GI toxicity, there was a similar distribution in genotypes: 90.9%, 91.9%, 90.9% for ThrThr, ThrMet, MetMet, respectively. PCa patients with ThrThr genotype had higher rate of acute GU toxicity grade ≥2 (45.5%) than ThrMet (28.6%) and MetMet (22.2%) while in GI toxicity MetMet had higher rate of grade ≥2 (40%) than ThrThr (23.3%) and ThrMet (23.5%) but without statistical significance. Conclusion The obtained data indicate that Thr variant of XRCC3 Thr241Met SNP is related to acute GU toxicity. Grade ≥2 acute GU toxicity could be associated with ThrThr while GI toxicity with MetMet genotype. Further study on larger group is necessary to confirm this date and to clarify mechanism underlying this observation.
PB  - Elsevier
C3  - ESTRO 2022 : Radiotherapy & Oncology : Journal of the European SocieTy for Radiotherapy and Oncology : Book of abstracts
T1  - XRCC3 Thr241Met gene polymorphism and acute radiotherapy induced toxicity for prostate cancer
VL  - 170
SP  - S1621
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12422
ER  - 

@conference{
author = "Mališić, Emina and Petrović, Nina and Nikitović, Marina",
year = "2022, S1",
abstract = "Purpose or Objective About half of all prostate cancer (PCa) patients receive radiotherapy (RT) either as single curative treatment or as adjuvant/salvage treatment after radical prostatectomy. However, RT is associated with a spectrum of side effects (toxicity) in the surrounding normal tissues. Acute toxicity occurs within 90 days of treatment, is usually transient and affects skin and mucosa of bladder and intestine resulting in dermatitis, cystitis or diarrhea. XRCC3 gene encodes for protein that is involved in homologous recombination repair of double-strand breaks created by ionizing radiation. Disruption of these pathways has the potential to affect the normal tissue response to RT. The T variant of XRCC3 Thr241Met single nucleotide polymorphisms (SNP) in exon 7 (C>T, rs861539) was reported to be associated with elevated levels of DNA adducts, chromosomal deletions, sensitivity to ionizing radiation and cross-linking agents. The aim of this study was to examine association between this SNP and RT-induced normal tissue toxicity in PCa patients. Materials and Methods Eighty one patients who had a histologically confirmed localized or locally advanced PCa were included in the study. Patients were treated with 3DC RT (n=70) or ARC RT (n=11) with radical (72 Gy)(47 patients) or postoperative/salvage (66 Gy)(34 patients) RT without previous hormonal therapy. DNA from peripheral blood mononuclear cells was extracted by salting out method. XRCC3 Thr241Met SNP was determined by PCR-RFLP analysis. The restriction fragments were separated on 2100 Bioanalyzer using DNA 1000 kit. The differences in the distribution of genotypes of XRCC3 Thr241Met between patients with or without acute RT-induced genitourinary (GU) or gastrointestinal (GI) toxicity were tested by χ2 and Fisher’s exact test. P values ≤ 0.05 were considered statistically significant, while p values between 0.1 and 0.05 were pointed out as a statistical trend. Data were analyzed by SigmaStat 3.5. Results The acute GI toxicity appeared in 100%, 94.6% and 81.8% of ThrThr, ThrMet and MetMet PCa patients, raspectivelly. There was the statistical trend towards higher acute GU toxicity in carriers of Thr variant (ThrThr plus ThrMet) vs. MetMet (p=0.087) as well as ThrThr vs. MetMet (p=0.058). For acute GI toxicity, there was a similar distribution in genotypes: 90.9%, 91.9%, 90.9% for ThrThr, ThrMet, MetMet, respectively. PCa patients with ThrThr genotype had higher rate of acute GU toxicity grade ≥2 (45.5%) than ThrMet (28.6%) and MetMet (22.2%) while in GI toxicity MetMet had higher rate of grade ≥2 (40%) than ThrThr (23.3%) and ThrMet (23.5%) but without statistical significance. Conclusion The obtained data indicate that Thr variant of XRCC3 Thr241Met SNP is related to acute GU toxicity. Grade ≥2 acute GU toxicity could be associated with ThrThr while GI toxicity with MetMet genotype. Further study on larger group is necessary to confirm this date and to clarify mechanism underlying this observation.",
publisher = "Elsevier",
journal = "ESTRO 2022 : Radiotherapy & Oncology : Journal of the European SocieTy for Radiotherapy and Oncology : Book of abstracts",
title = "XRCC3 Thr241Met gene polymorphism and acute radiotherapy induced toxicity for prostate cancer",
volume = "170",
pages = "S1621",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12422"
}

Mališić, E., Petrović, N.,& Nikitović, M.. (2022). XRCC3 Thr241Met gene polymorphism and acute radiotherapy induced toxicity for prostate cancer. in ESTRO 2022 : Radiotherapy & Oncology : Journal of the European SocieTy for Radiotherapy and Oncology : Book of abstracts
Elsevier., 170, S1621.
https://hdl.handle.net/21.15107/rcub_vinar_12422

Mališić E, Petrović N, Nikitović M. XRCC3 Thr241Met gene polymorphism and acute radiotherapy induced toxicity for prostate cancer. in ESTRO 2022 : Radiotherapy & Oncology : Journal of the European SocieTy for Radiotherapy and Oncology : Book of abstracts. 2022;170:S1621.
https://hdl.handle.net/21.15107/rcub_vinar_12422 .

Mališić, Emina, Petrović, Nina, Nikitović, Marina, "XRCC3 Thr241Met gene polymorphism and acute radiotherapy induced toxicity for prostate cancer" in ESTRO 2022 : Radiotherapy & Oncology : Journal of the European SocieTy for Radiotherapy and Oncology : Book of abstracts, 170 (2022):S1621,
https://hdl.handle.net/21.15107/rcub_vinar_12422 .

TY  - CONF
AU  - Petrović, Nina
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12457
C3  - Annals of cancerology section : 59th Cancerology Week : Book of asbtracts
T1  - Response to radiotherapy: transcript analysis, tests for prediction of toxicity and radiosensitivity, and potential application in clinical practice
T1  - Odgovor na radioterapiju: analiza transkripta, testovi za predikciju toksičnosti i radiosenzitivnosti i primena u kliničkoj praksi
SP  - 111
EP  - 114
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12457
ER  - 

@conference{
author = "Petrović, Nina",
year = "2022",
journal = "Annals of cancerology section : 59th Cancerology Week : Book of asbtracts",
title = "Response to radiotherapy: transcript analysis, tests for prediction of toxicity and radiosensitivity, and potential application in clinical practice, Odgovor na radioterapiju: analiza transkripta, testovi za predikciju toksičnosti i radiosenzitivnosti i primena u kliničkoj praksi",
pages = "111-114",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12457"
}

Petrović, N.. (2022). Response to radiotherapy: transcript analysis, tests for prediction of toxicity and radiosensitivity, and potential application in clinical practice. in Annals of cancerology section : 59th Cancerology Week : Book of asbtracts, 111-114.
https://hdl.handle.net/21.15107/rcub_vinar_12457

Petrović N. Response to radiotherapy: transcript analysis, tests for prediction of toxicity and radiosensitivity, and potential application in clinical practice. in Annals of cancerology section : 59th Cancerology Week : Book of asbtracts. 2022;:111-114.
https://hdl.handle.net/21.15107/rcub_vinar_12457 .

Petrović, Nina, "Response to radiotherapy: transcript analysis, tests for prediction of toxicity and radiosensitivity, and potential application in clinical practice" in Annals of cancerology section : 59th Cancerology Week : Book of asbtracts (2022):111-114,
https://hdl.handle.net/21.15107/rcub_vinar_12457 .

TY  - CONF
AU  - Stepanović, Aleksandar
AU  - Petrović, Nina
AU  - Ilić, Rosanda
AU  - Bogdanović, Ivan
AU  - Arsenijević, Tatjana
AU  - Grujičić, Danica
AU  - Nikitović, Marina
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12458
C3  - Annals of cancerology section : 59th Cancerology Week : Book of asbtracts
T1  - MikroRNK kao prediktori radiotoksičnosti kod pacijenata sa glioblastomom
SP  - 114
EP  - 115
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12458
ER  - 

@conference{
author = "Stepanović, Aleksandar and Petrović, Nina and Ilić, Rosanda and Bogdanović, Ivan and Arsenijević, Tatjana and Grujičić, Danica and Nikitović, Marina",
year = "2022",
journal = "Annals of cancerology section : 59th Cancerology Week : Book of asbtracts",
title = "MikroRNK kao prediktori radiotoksičnosti kod pacijenata sa glioblastomom",
pages = "114-115",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12458"
}

Stepanović, A., Petrović, N., Ilić, R., Bogdanović, I., Arsenijević, T., Grujičić, D.,& Nikitović, M.. (2022). MikroRNK kao prediktori radiotoksičnosti kod pacijenata sa glioblastomom. in Annals of cancerology section : 59th Cancerology Week : Book of asbtracts, 114-115.
https://hdl.handle.net/21.15107/rcub_vinar_12458

Stepanović A, Petrović N, Ilić R, Bogdanović I, Arsenijević T, Grujičić D, Nikitović M. MikroRNK kao prediktori radiotoksičnosti kod pacijenata sa glioblastomom. in Annals of cancerology section : 59th Cancerology Week : Book of asbtracts. 2022;:114-115.
https://hdl.handle.net/21.15107/rcub_vinar_12458 .

Stepanović, Aleksandar, Petrović, Nina, Ilić, Rosanda, Bogdanović, Ivan, Arsenijević, Tatjana, Grujičić, Danica, Nikitović, Marina, "MikroRNK kao prediktori radiotoksičnosti kod pacijenata sa glioblastomom" in Annals of cancerology section : 59th Cancerology Week : Book of asbtracts (2022):114-115,
https://hdl.handle.net/21.15107/rcub_vinar_12458 .

TY  - JOUR
AU  - Vučetić, Milan
AU  - Roganović, Jelena
AU  - Freilich, M.
AU  - Shafer, D.
AU  - Milić, Marija
AU  - Đukić, Ljiljana
AU  - Petrović, Nina
AU  - Marković, Evgenija
AU  - Marković, Aleksa
AU  - Brković, Božidar
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13113
AB  - Objective: To evaluate an association between bone levels of inflammation/oxidative stress mediators and postoperative discomfort after third molar conventional or piezosurgery. Material and methods: Twenty-six subjects with bilaterally impacted mandibular third molars, who underwent either piezo or conventional surgery, were included in a split-mouth design study. MicroRNA-21 (miR-21) expression, interleukin-1 beta (IL-1β), and vascular endothelial growth factor (VEGF) proteins, as well as superoxide dismutase (SOD) activity in alveolar bone, were evaluated. Pain intensity, the first pain appearance, analgesic first use and total dose taken, trismus, and swelling were clinically recorded. Results: MiR-21 expression was higher while VEGF protein was lower in piezosurgery vs. conventional groups. The differences in IL-1β protein and SOD activity were not significant between groups. The pain intensity on the first day was significantly decreased in piezosurgery group. The first pain appearance and the first analgesic taken were reported sooner in conventional vs. piezosurgical group. Significantly pronounced trismus on the third day following conventional surgery was found. In conventional group, significantly increased trismus was observed on the third compared to the first postoperative day. MiR-21 showed significant correlation with the first pain appearance. Conclusion: Delayed onset of less pronounced postoperative pain after piezosurgical vs. conventional extraction of impacted lower third molar was significantly associated with expression of bone miR-21. Clinical relevance: Alveolar bone miR-21 may reflect surgical stress and is associated with third molar postoperative pain onset.
T2  - Clinical Oral Investigations
T1  - Bone microRNA-21 as surgical stress parameter is associated with third molar postoperative discomfort
VL  - 25
IS  - 1
SP  - 319
EP  - 328
DO  - 10.1007/s00784-020-03366-6
ER  - 

@article{
author = "Vučetić, Milan and Roganović, Jelena and Freilich, M. and Shafer, D. and Milić, Marija and Đukić, Ljiljana and Petrović, Nina and Marković, Evgenija and Marković, Aleksa and Brković, Božidar",
year = "2021",
abstract = "Objective: To evaluate an association between bone levels of inflammation/oxidative stress mediators and postoperative discomfort after third molar conventional or piezosurgery. Material and methods: Twenty-six subjects with bilaterally impacted mandibular third molars, who underwent either piezo or conventional surgery, were included in a split-mouth design study. MicroRNA-21 (miR-21) expression, interleukin-1 beta (IL-1β), and vascular endothelial growth factor (VEGF) proteins, as well as superoxide dismutase (SOD) activity in alveolar bone, were evaluated. Pain intensity, the first pain appearance, analgesic first use and total dose taken, trismus, and swelling were clinically recorded. Results: MiR-21 expression was higher while VEGF protein was lower in piezosurgery vs. conventional groups. The differences in IL-1β protein and SOD activity were not significant between groups. The pain intensity on the first day was significantly decreased in piezosurgery group. The first pain appearance and the first analgesic taken were reported sooner in conventional vs. piezosurgical group. Significantly pronounced trismus on the third day following conventional surgery was found. In conventional group, significantly increased trismus was observed on the third compared to the first postoperative day. MiR-21 showed significant correlation with the first pain appearance. Conclusion: Delayed onset of less pronounced postoperative pain after piezosurgical vs. conventional extraction of impacted lower third molar was significantly associated with expression of bone miR-21. Clinical relevance: Alveolar bone miR-21 may reflect surgical stress and is associated with third molar postoperative pain onset.",
journal = "Clinical Oral Investigations",
title = "Bone microRNA-21 as surgical stress parameter is associated with third molar postoperative discomfort",
volume = "25",
number = "1",
pages = "319-328",
doi = "10.1007/s00784-020-03366-6"
}

Vučetić, M., Roganović, J., Freilich, M., Shafer, D., Milić, M., Đukić, L., Petrović, N., Marković, E., Marković, A.,& Brković, B.. (2021). Bone microRNA-21 as surgical stress parameter is associated with third molar postoperative discomfort. in Clinical Oral Investigations, 25(1), 319-328.
https://doi.org/10.1007/s00784-020-03366-6

Vučetić M, Roganović J, Freilich M, Shafer D, Milić M, Đukić L, Petrović N, Marković E, Marković A, Brković B. Bone microRNA-21 as surgical stress parameter is associated with third molar postoperative discomfort. in Clinical Oral Investigations. 2021;25(1):319-328.
doi:10.1007/s00784-020-03366-6 .

Vučetić, Milan, Roganović, Jelena, Freilich, M., Shafer, D., Milić, Marija, Đukić, Ljiljana, Petrović, Nina, Marković, Evgenija, Marković, Aleksa, Brković, Božidar, "Bone microRNA-21 as surgical stress parameter is associated with third molar postoperative discomfort" in Clinical Oral Investigations, 25, no. 1 (2021):319-328,
https://doi.org/10.1007/s00784-020-03366-6 . .

TY  - JOUR
AU  - Petronijević, Jelena
AU  - Joksimović, Nenad
AU  - Milović, Emilija
AU  - Đorđić Crnogorac, Marija
AU  - Petrović, Nina
AU  - Stanojković, Tatjana P.
AU  - Milivojević, Dušan
AU  - Janković, Nenad
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9933
AB  - In order to discover new therapeutically active agents a series of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones were synthesized. All complexes were characterized by IR and EPR spectroscopic techniques and examined for their cytotoxic effect on human cancer cell lines HeLa, LS174, A549 and normal fibroblasts (MRC-5). For further examination of the cytotoxic mechanisms of novel complexes, three of them were chosen for analysing their effects on the distribution of HeLa cells in the cell cycle phases. The results of the flow cytometry analysis suggest that tested complexes lead to time-dependent accumulation of the cells in S and G2/M phases. The strongest accumulation effect showed complex 2d after 48 h of incubation. Competitive experiments with ethidium bromide (EB) indicated that tested compound 2d have affinity to displace EB from the EB-DNA complex through intercalation. Also, the binding parameters values for 2d-BSA complex showed that a reversible 2d-BSA complex is formed and ligand 2d can be stored and carried by BSA.
T2  - Chemico-Biological Interactions
T1  - Antitumor activity, DNA and BSA interactions of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones
VL  - 348
SP  - 109647
DO  - 10.1016/j.cbi.2021.109647
ER  - 

@article{
author = "Petronijević, Jelena and Joksimović, Nenad and Milović, Emilija and Đorđić Crnogorac, Marija and Petrović, Nina and Stanojković, Tatjana P. and Milivojević, Dušan and Janković, Nenad",
year = "2021",
abstract = "In order to discover new therapeutically active agents a series of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones were synthesized. All complexes were characterized by IR and EPR spectroscopic techniques and examined for their cytotoxic effect on human cancer cell lines HeLa, LS174, A549 and normal fibroblasts (MRC-5). For further examination of the cytotoxic mechanisms of novel complexes, three of them were chosen for analysing their effects on the distribution of HeLa cells in the cell cycle phases. The results of the flow cytometry analysis suggest that tested complexes lead to time-dependent accumulation of the cells in S and G2/M phases. The strongest accumulation effect showed complex 2d after 48 h of incubation. Competitive experiments with ethidium bromide (EB) indicated that tested compound 2d have affinity to displace EB from the EB-DNA complex through intercalation. Also, the binding parameters values for 2d-BSA complex showed that a reversible 2d-BSA complex is formed and ligand 2d can be stored and carried by BSA.",
journal = "Chemico-Biological Interactions",
title = "Antitumor activity, DNA and BSA interactions of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones",
volume = "348",
pages = "109647",
doi = "10.1016/j.cbi.2021.109647"
}

Petronijević, J., Joksimović, N., Milović, E., Đorđić Crnogorac, M., Petrović, N., Stanojković, T. P., Milivojević, D.,& Janković, N.. (2021). Antitumor activity, DNA and BSA interactions of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones. in Chemico-Biological Interactions, 348, 109647.
https://doi.org/10.1016/j.cbi.2021.109647

Petronijević J, Joksimović N, Milović E, Đorđić Crnogorac M, Petrović N, Stanojković TP, Milivojević D, Janković N. Antitumor activity, DNA and BSA interactions of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones. in Chemico-Biological Interactions. 2021;348:109647.
doi:10.1016/j.cbi.2021.109647 .

Petronijević, Jelena, Joksimović, Nenad, Milović, Emilija, Đorđić Crnogorac, Marija, Petrović, Nina, Stanojković, Tatjana P., Milivojević, Dušan, Janković, Nenad, "Antitumor activity, DNA and BSA interactions of novel copper(II) complexes with 3,4-dihydro-2(1H)-quinoxalinones" in Chemico-Biological Interactions, 348 (2021):109647,
https://doi.org/10.1016/j.cbi.2021.109647 . .

TY  - JOUR
AU  - Vukotić, Aleksandra
AU  - Jevđić, Jasna
AU  - Green, David
AU  - Vukotić, Milovan
AU  - Petrović, Nina
AU  - Janićijević, Ana
AU  - Nenadić, Irina
AU  - Boboš, Marina
AU  - Čuljić, Radmila
AU  - Zagorac, Zagor
AU  - Stevanović, Predrag
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9923
AB  - Introduction/Objective. Despite frequent side effects such as hypotension, spinal anesthesia (SA) is still one of the best anesthetic methods for elective cesarean section (CS). Intermittent, oscillometric, noninvasive blood pressure monitoring (NIBP) frequently leads to missed hypotensive episodes. The objective was to compare continuous non-invasive arterial pressure (CNAP) monitoring with NIBP in the terms of efficiency to detect hypotension. Methods. In this study, we compared CNAP and NIBP monitoring for hypotension detection in 76 patients divided into two groups of 38 patients treated with ephedrine (E) or phenylephrine (P), during threeminute intervals, starting from SA, by the end of the surgery. Results. In E group, significantly lower mean systolic blood pressure (SBP) values with CNAP compared with NIBP (p = 0.008) was detected. By monitoring CNAP, we detected 31 (81.6%) hypotensive patients in E group and significantly lower number, 20 (52.6%), with NIBP (p = 0.001), while in P group CNAP detected 34 patients (89.5%) and NIBP only 18 (47.3%), p = 0.001. By monitoring CNAP, we detected significantly higher number of hypotensive intervals in E and P groups (p
AB  - Увод/Циљ Упркос честим нежељеним ефектима као што је хипотензија, спинална анестезија је и даље техника избора за планирани царски рез. Интермитентно неинвазивно праћење крвног притиска често не детектује хипотензивне епизоде. Циљ ове студије је био да се упореди континуирано неинвазивно праћење артеријског притиска са интермитентним неинвазивним праћењем крвног притиска у смислу ефикасности у откривању хипотензије.Методе Упоређивани су системи за континуирано неинвазивно праћење артеријског притиска и интермитентно неинвазивно праћење крвног притиска ради детекције хипотензије код 76 болесница подељених у две групе од по 38 болесница, третираних ефедрином (Е) или фенилефрином (Ф), на свака три минута, почевши од спиналне анестезије па све до краја операције.Резултати У групи Е су детектоване знатно ниже средње вредности систолног крвног притиска континуираним неинвазивним праћењем артеријског притиска у поређењу са интермитентним неинвазивним праћењем крвног притиска (p = 0,008). Континуираним неинвазивним праћењем артеријског притиска детектована је 31 (81,6%) хипотензивна болесница у групи Е и знатно мањи број, 20 (52,6%) болесница, интермитентним неинвазивним праћењем крвног притиска (p = 0,001), док је у групи Ф континуираним неинвазивним праћењем артеријског притиска детектована хипотензија код 34 болеснице (89,5%), а интермитентним неинвазивним праћењем крвног притиска код 18 (47,3%) болесница,  p = 0,001. Континуираним неинвазивним праћењем артеријског притиска детектован је знатно већи број хипотензивних епизода у групама Е и Ф (p < 0,001). pH вредности умбиликалне крви биле су значајно ниже код хипотензивних у односу на нормотензивне болеснице у групама Е и Ф, и са континуираним неинвазивним праћењем артеријског притиска и интермитентним неинвазивним праћењем крвног притиска, респективно (p < 0,001, p = 0,027 у групи Е, и p = 0,009, p < 0,001 у групи Ф).Закључак Континуирано неинвазивно праћење артеријског притиска је много ефикасније у откривању хипотензије током царског реза у спиналној анестезији, што омогућава бржи третман и мање нежељених ефеката код мајке и новорођенчета.
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Detection of hypotension during spinal anesthesia for caesarean section with continuous non-invasive arterial pressure monitoring and intermittent oscillometric blood pressure monitoring in patients treated with ephedrine or phenylephrine
VL  - 149
IS  - 7-8
SP  - 442
EP  - 448
DO  - 10.2298/SARH200317030V
ER  - 

@article{
author = "Vukotić, Aleksandra and Jevđić, Jasna and Green, David and Vukotić, Milovan and Petrović, Nina and Janićijević, Ana and Nenadić, Irina and Boboš, Marina and Čuljić, Radmila and Zagorac, Zagor and Stevanović, Predrag",
year = "2021",
abstract = "Introduction/Objective. Despite frequent side effects such as hypotension, spinal anesthesia (SA) is still one of the best anesthetic methods for elective cesarean section (CS). Intermittent, oscillometric, noninvasive blood pressure monitoring (NIBP) frequently leads to missed hypotensive episodes. The objective was to compare continuous non-invasive arterial pressure (CNAP) monitoring with NIBP in the terms of efficiency to detect hypotension. Methods. In this study, we compared CNAP and NIBP monitoring for hypotension detection in 76 patients divided into two groups of 38 patients treated with ephedrine (E) or phenylephrine (P), during threeminute intervals, starting from SA, by the end of the surgery. Results. In E group, significantly lower mean systolic blood pressure (SBP) values with CNAP compared with NIBP (p = 0.008) was detected. By monitoring CNAP, we detected 31 (81.6%) hypotensive patients in E group and significantly lower number, 20 (52.6%), with NIBP (p = 0.001), while in P group CNAP detected 34 patients (89.5%) and NIBP only 18 (47.3%), p = 0.001. By monitoring CNAP, we detected significantly higher number of hypotensive intervals in E and P groups (p, Увод/Циљ Упркос честим нежељеним ефектима као што је хипотензија, спинална анестезија је и даље техника избора за планирани царски рез. Интермитентно неинвазивно праћење крвног притиска често не детектује хипотензивне епизоде. Циљ ове студије је био да се упореди континуирано неинвазивно праћење артеријског притиска са интермитентним неинвазивним праћењем крвног притиска у смислу ефикасности у откривању хипотензије.Методе Упоређивани су системи за континуирано неинвазивно праћење артеријског притиска и интермитентно неинвазивно праћење крвног притиска ради детекције хипотензије код 76 болесница подељених у две групе од по 38 болесница, третираних ефедрином (Е) или фенилефрином (Ф), на свака три минута, почевши од спиналне анестезије па све до краја операције.Резултати У групи Е су детектоване знатно ниже средње вредности систолног крвног притиска континуираним неинвазивним праћењем артеријског притиска у поређењу са интермитентним неинвазивним праћењем крвног притиска (p = 0,008). Континуираним неинвазивним праћењем артеријског притиска детектована је 31 (81,6%) хипотензивна болесница у групи Е и знатно мањи број, 20 (52,6%) болесница, интермитентним неинвазивним праћењем крвног притиска (p = 0,001), док је у групи Ф континуираним неинвазивним праћењем артеријског притиска детектована хипотензија код 34 болеснице (89,5%), а интермитентним неинвазивним праћењем крвног притиска код 18 (47,3%) болесница,  p = 0,001. Континуираним неинвазивним праћењем артеријског притиска детектован је знатно већи број хипотензивних епизода у групама Е и Ф (p < 0,001). pH вредности умбиликалне крви биле су значајно ниже код хипотензивних у односу на нормотензивне болеснице у групама Е и Ф, и са континуираним неинвазивним праћењем артеријског притиска и интермитентним неинвазивним праћењем крвног притиска, респективно (p < 0,001, p = 0,027 у групи Е, и p = 0,009, p < 0,001 у групи Ф).Закључак Континуирано неинвазивно праћење артеријског притиска је много ефикасније у откривању хипотензије током царског реза у спиналној анестезији, што омогућава бржи третман и мање нежељених ефеката код мајке и новорођенчета.",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Detection of hypotension during spinal anesthesia for caesarean section with continuous non-invasive arterial pressure monitoring and intermittent oscillometric blood pressure monitoring in patients treated with ephedrine or phenylephrine",
volume = "149",
number = "7-8",
pages = "442-448",
doi = "10.2298/SARH200317030V"
}

Vukotić, A., Jevđić, J., Green, D., Vukotić, M., Petrović, N., Janićijević, A., Nenadić, I., Boboš, M., Čuljić, R., Zagorac, Z.,& Stevanović, P.. (2021). Detection of hypotension during spinal anesthesia for caesarean section with continuous non-invasive arterial pressure monitoring and intermittent oscillometric blood pressure monitoring in patients treated with ephedrine or phenylephrine. in Srpski arhiv za celokupno lekarstvo, 149(7-8), 442-448.
https://doi.org/10.2298/SARH200317030V

Vukotić A, Jevđić J, Green D, Vukotić M, Petrović N, Janićijević A, Nenadić I, Boboš M, Čuljić R, Zagorac Z, Stevanović P. Detection of hypotension during spinal anesthesia for caesarean section with continuous non-invasive arterial pressure monitoring and intermittent oscillometric blood pressure monitoring in patients treated with ephedrine or phenylephrine. in Srpski arhiv za celokupno lekarstvo. 2021;149(7-8):442-448.
doi:10.2298/SARH200317030V .

Vukotić, Aleksandra, Jevđić, Jasna, Green, David, Vukotić, Milovan, Petrović, Nina, Janićijević, Ana, Nenadić, Irina, Boboš, Marina, Čuljić, Radmila, Zagorac, Zagor, Stevanović, Predrag, "Detection of hypotension during spinal anesthesia for caesarean section with continuous non-invasive arterial pressure monitoring and intermittent oscillometric blood pressure monitoring in patients treated with ephedrine or phenylephrine" in Srpski arhiv za celokupno lekarstvo, 149, no. 7-8 (2021):442-448,
https://doi.org/10.2298/SARH200317030V . .