TY  - CONF
AU  - Đorđević, Neda O.
AU  - Keta, Otilija D.
AU  - Petković, Vladana
AU  - Todorović Vukotić, Nevena
AU  - Stanković, Dalibor
AU  - Tešević, V.
AU  - Pajović, Snežana B.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12594
AB  - This study aimed to determine the phenolic compound content, in vitro antioxidative potential, and cytotoxic effects of four red wine samples: a commercial (V) and three clonal wines (V1, V2, and V3). LC/MS-MS, cyclic voltammetry, and MTT assay techniques were employed for this purpose. Results revealed that all wines were rich in phenolic compounds. Clonal wines outperformed the commercial ones in most phenolic compounds (except myricetin). Notably, V2 and V3 showed the highest levels of gallic acid, catechin, and epicatechin. Among them, V3 exhibited superior antioxidative activity. The MTT assay demonstrated stronger cytotoxic effects of the wine samples on pancreas (Bx-PC3) and colon (HT29) carcinoma cells (47% to 16% and 27% to 7% compared to control, respectively) than on the normal lung fibroblasts (MRC-5) cell line (106% to 77%). It can be concluded that HT29 cells were more sensitive than Bx-PC3 cells. Finally, both clonal and commercial wines serve as valuable sources of polyphenolic compounds, which could have a significant role in preventing cancer and diseases related to oxidative stress.
PB  - Kragujevac : Institute for Information Technologies, University of Kragujevac
C3  - ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
T1  - In vitro biological effects of clonal red wines
SP  - 180
EP  - 183
DO  - 10.46793/ICCBI23.180DJ
ER  - 

@conference{
author = "Đorđević, Neda O. and Keta, Otilija D. and Petković, Vladana and Todorović Vukotić, Nevena and Stanković, Dalibor and Tešević, V. and Pajović, Snežana B.",
year = "2023",
abstract = "This study aimed to determine the phenolic compound content, in vitro antioxidative potential, and cytotoxic effects of four red wine samples: a commercial (V) and three clonal wines (V1, V2, and V3). LC/MS-MS, cyclic voltammetry, and MTT assay techniques were employed for this purpose. Results revealed that all wines were rich in phenolic compounds. Clonal wines outperformed the commercial ones in most phenolic compounds (except myricetin). Notably, V2 and V3 showed the highest levels of gallic acid, catechin, and epicatechin. Among them, V3 exhibited superior antioxidative activity. The MTT assay demonstrated stronger cytotoxic effects of the wine samples on pancreas (Bx-PC3) and colon (HT29) carcinoma cells (47% to 16% and 27% to 7% compared to control, respectively) than on the normal lung fibroblasts (MRC-5) cell line (106% to 77%). It can be concluded that HT29 cells were more sensitive than Bx-PC3 cells. Finally, both clonal and commercial wines serve as valuable sources of polyphenolic compounds, which could have a significant role in preventing cancer and diseases related to oxidative stress.",
publisher = "Kragujevac : Institute for Information Technologies, University of Kragujevac",
journal = "ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings",
title = "In vitro biological effects of clonal red wines",
pages = "180-183",
doi = "10.46793/ICCBI23.180DJ"
}

Đorđević, N. O., Keta, O. D., Petković, V., Todorović Vukotić, N., Stanković, D., Tešević, V.,& Pajović, S. B.. (2023). In vitro biological effects of clonal red wines. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
Kragujevac : Institute for Information Technologies, University of Kragujevac., 180-183.
https://doi.org/10.46793/ICCBI23.180DJ

Đorđević NO, Keta OD, Petković V, Todorović Vukotić N, Stanković D, Tešević V, Pajović SB. In vitro biological effects of clonal red wines. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings. 2023;:180-183.
doi:10.46793/ICCBI23.180DJ .

Đorđević, Neda O., Keta, Otilija D., Petković, Vladana, Todorović Vukotić, Nevena, Stanković, Dalibor, Tešević, V., Pajović, Snežana B., "In vitro biological effects of clonal red wines" in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings (2023):180-183,
https://doi.org/10.46793/ICCBI23.180DJ . .

TY  - JOUR
AU  - Đorđević, Neda O.
AU  - Stanisavljević, Nemanja
AU  - Todorović Vukotić, Nevena
AU  - Novović, Katarina
AU  - Žakula, Jelena
AU  - Stanković, Dalibor M.
AU  - Pajović, Snežana B.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10353
AB  - The beneficial effect of moderate wine consumption is attributed to its micronutrients, especially polyphenols and largely depends on the digestion process. This work aimed to examine the influence of in vitro simulated digestion in the presence of complex food matrix on antioxidant and cytotoxic activity of red wine. The obtained results showed that total phenolic content of wine sample after in vitro digestion was higher compared to undigested wine, while the antioxidant activity of these samples was similar before and after digestion. Furthermore, it has been noticed that digested wine showed cytotoxic activity on SKBR3 breast adenocarcinoma cells near 20% after 72 h of treatment. This pioneering study that examined biological potential of in vitro digested wine in the presence of complex food matrix indicate that antioxidant and cytotoxic activity of red wine is preserved after digestion.
T2  - Natural Product Research
T1  - Antioxidant and cytotoxic activity of red wine after in vitro simulated digestion in the presence of complex food matrix
SP  - 1
EP  - 6
DO  - 10.1080/14786419.2022.2095380
ER  - 

@article{
author = "Đorđević, Neda O. and Stanisavljević, Nemanja and Todorović Vukotić, Nevena and Novović, Katarina and Žakula, Jelena and Stanković, Dalibor M. and Pajović, Snežana B.",
year = "2022",
abstract = "The beneficial effect of moderate wine consumption is attributed to its micronutrients, especially polyphenols and largely depends on the digestion process. This work aimed to examine the influence of in vitro simulated digestion in the presence of complex food matrix on antioxidant and cytotoxic activity of red wine. The obtained results showed that total phenolic content of wine sample after in vitro digestion was higher compared to undigested wine, while the antioxidant activity of these samples was similar before and after digestion. Furthermore, it has been noticed that digested wine showed cytotoxic activity on SKBR3 breast adenocarcinoma cells near 20% after 72 h of treatment. This pioneering study that examined biological potential of in vitro digested wine in the presence of complex food matrix indicate that antioxidant and cytotoxic activity of red wine is preserved after digestion.",
journal = "Natural Product Research",
title = "Antioxidant and cytotoxic activity of red wine after in vitro simulated digestion in the presence of complex food matrix",
pages = "1-6",
doi = "10.1080/14786419.2022.2095380"
}

Đorđević, N. O., Stanisavljević, N., Todorović Vukotić, N., Novović, K., Žakula, J., Stanković, D. M.,& Pajović, S. B.. (2022). Antioxidant and cytotoxic activity of red wine after in vitro simulated digestion in the presence of complex food matrix. in Natural Product Research, 1-6.
https://doi.org/10.1080/14786419.2022.2095380

Đorđević NO, Stanisavljević N, Todorović Vukotić N, Novović K, Žakula J, Stanković DM, Pajović SB. Antioxidant and cytotoxic activity of red wine after in vitro simulated digestion in the presence of complex food matrix. in Natural Product Research. 2022;:1-6.
doi:10.1080/14786419.2022.2095380 .

Đorđević, Neda O., Stanisavljević, Nemanja, Todorović Vukotić, Nevena, Novović, Katarina, Žakula, Jelena, Stanković, Dalibor M., Pajović, Snežana B., "Antioxidant and cytotoxic activity of red wine after in vitro simulated digestion in the presence of complex food matrix" in Natural Product Research (2022):1-6,
https://doi.org/10.1080/14786419.2022.2095380 . .

TY  - CONF
AU  - Đorđević, Neda
AU  - Todorović Vukotić, Nevena
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Pejić, Snežana
AU  - Pajović, Snežana B.
AU  - Tešević, V.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12586
AB  - Positive effects of moderate wine consumption in individuals with cardiovascular disease, hypertension, diabetes, and cancers have been shown in numerous epidemiological and clinical studies. This research examined the phenolic content of commercial and two clonal Merlot wines as well as their biological potential. The obtained results indicated that all analyzed samples were a good source of phenolic compounds. Cytotoxicity assay on melanoma (A375) and cervical (HeLa) cancer cell lines have shown that all analyzed wines inhibited the growth of human cancer cells in vitro with differing susceptibility among tested cell lines. Clonal wines in the volume ratio of 10 and 20% showed to be more efficient anti-proliferative agents than commercial wine regarding the A375 cells. This could be connected with higher total phenolic content in clonal wines. The effect of all analyzed samples on the A375 cells was greater compared to HeLa cell line.
PB  - Belgrade : Society of Physical Chemists of Serbia
C3  - 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2022 : Book of Abstracts
T1  - Phenolic Composition and Cytotoxic Activity of Red Wine
SP  - 153
EP  - 153
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12586
ER  - 

@conference{
author = "Đorđević, Neda and Todorović Vukotić, Nevena and Korićanac, Lela and Žakula, Jelena and Pejić, Snežana and Pajović, Snežana B. and Tešević, V.",
year = "2022",
abstract = "Positive effects of moderate wine consumption in individuals with cardiovascular disease, hypertension, diabetes, and cancers have been shown in numerous epidemiological and clinical studies. This research examined the phenolic content of commercial and two clonal Merlot wines as well as their biological potential. The obtained results indicated that all analyzed samples were a good source of phenolic compounds. Cytotoxicity assay on melanoma (A375) and cervical (HeLa) cancer cell lines have shown that all analyzed wines inhibited the growth of human cancer cells in vitro with differing susceptibility among tested cell lines. Clonal wines in the volume ratio of 10 and 20% showed to be more efficient anti-proliferative agents than commercial wine regarding the A375 cells. This could be connected with higher total phenolic content in clonal wines. The effect of all analyzed samples on the A375 cells was greater compared to HeLa cell line.",
publisher = "Belgrade : Society of Physical Chemists of Serbia",
journal = "16th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2022 : Book of Abstracts",
title = "Phenolic Composition and Cytotoxic Activity of Red Wine",
pages = "153-153",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12586"
}

Đorđević, N., Todorović Vukotić, N., Korićanac, L., Žakula, J., Pejić, S., Pajović, S. B.,& Tešević, V.. (2022). Phenolic Composition and Cytotoxic Activity of Red Wine. in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2022 : Book of Abstracts
Belgrade : Society of Physical Chemists of Serbia., 153-153.
https://hdl.handle.net/21.15107/rcub_vinar_12586

Đorđević N, Todorović Vukotić N, Korićanac L, Žakula J, Pejić S, Pajović SB, Tešević V. Phenolic Composition and Cytotoxic Activity of Red Wine. in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2022 : Book of Abstracts. 2022;:153-153.
https://hdl.handle.net/21.15107/rcub_vinar_12586 .

Đorđević, Neda, Todorović Vukotić, Nevena, Korićanac, Lela, Žakula, Jelena, Pejić, Snežana, Pajović, Snežana B., Tešević, V., "Phenolic Composition and Cytotoxic Activity of Red Wine" in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2022 : Book of Abstracts (2022):153-153,
https://hdl.handle.net/21.15107/rcub_vinar_12586 .

TY  - CONF
AU  - Đorđević, Neda
AU  - Todorović Vukotić, Nevena
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Pejić, Snežana
AU  - Tešević, V.
AU  - Pajović, Snežana B.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12592
AB  - Benefitni efekat crvenog vina na zdravlje ljudi od davnina je poznat. Među alkoholnim pićima crveno vino pokazuje najjači efekat protiv pojave oboljenja povezanih sa oksidativnim stresom, kao što su kardiovaskularna, neurodegenerativna oboljenja, dijabetes i kancer.1 U okviru ove studije ispitan je citotoksični efekat crvenog vina sorte merlo (komercijalno dostupno vino i vina dobijena od klonova VCR1 i VCR101) poreklom iz Crne Gore, berba 2011, u odnosu na ćelijsku liniju kancera pankreasa (PANC-1) i ćelijsku liniju melanoma (A375). Citotoksični efekat vina je određen SRB metodom kroz praćenje ćelijskog preživljavanja kancerskih ćelija 48 sati nakon tretmana sa tri različita zapreminska procenta analiziranih vina (5, 10 i 20%). Dobijeni rezultati su pokazali citotoksični efekat svih analiziranih vina na obe ćelijske linije. Ćelijsko preživljavanje nakon tretmana vinima se kretalo od 36 do 64%. Citotoksični efekat svih vina u odnosu na A375 ćelije je bio veći nego na PANC-1 ćelijsku liniju, što je od posebnog značaja ako se uzme u obzir rezistentnost melanomskih ćelija na postojeće terapeutske tretmane. Takođe, klonska sortna vina su pokazala veći citotoksični efekat na A375 ćelijsku liniju od komercijalno dostupnog vina. Dobijeni rezultati su ukazali da umereno konzumiranje crvenog vina sorte merlo sa specifičnim geografskim poreklom predstavlja dobar izvor bioaktivnih komponenata sa pozitivnim biološkim efektom, što je od posebne važnosti za klonska sortna vina.
PB  - Beograd : Srpsko biološko društvo
C3  - 3. kongres biologa Srbije : osnovna i primenjena istraživanja, metodika nastave : knjiga sažetaka
T1  - Citotoksično dejstvo crvenog vina na tumorske ćelije u in vitro uslovima
SP  - 321
EP  - 321
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12592
ER  - 

@conference{
author = "Đorđević, Neda and Todorović Vukotić, Nevena and Korićanac, Lela and Žakula, Jelena and Pejić, Snežana and Tešević, V. and Pajović, Snežana B.",
year = "2022",
abstract = "Benefitni efekat crvenog vina na zdravlje ljudi od davnina je poznat. Među alkoholnim pićima crveno vino pokazuje najjači efekat protiv pojave oboljenja povezanih sa oksidativnim stresom, kao što su kardiovaskularna, neurodegenerativna oboljenja, dijabetes i kancer.1 U okviru ove studije ispitan je citotoksični efekat crvenog vina sorte merlo (komercijalno dostupno vino i vina dobijena od klonova VCR1 i VCR101) poreklom iz Crne Gore, berba 2011, u odnosu na ćelijsku liniju kancera pankreasa (PANC-1) i ćelijsku liniju melanoma (A375). Citotoksični efekat vina je određen SRB metodom kroz praćenje ćelijskog preživljavanja kancerskih ćelija 48 sati nakon tretmana sa tri različita zapreminska procenta analiziranih vina (5, 10 i 20%). Dobijeni rezultati su pokazali citotoksični efekat svih analiziranih vina na obe ćelijske linije. Ćelijsko preživljavanje nakon tretmana vinima se kretalo od 36 do 64%. Citotoksični efekat svih vina u odnosu na A375 ćelije je bio veći nego na PANC-1 ćelijsku liniju, što je od posebnog značaja ako se uzme u obzir rezistentnost melanomskih ćelija na postojeće terapeutske tretmane. Takođe, klonska sortna vina su pokazala veći citotoksični efekat na A375 ćelijsku liniju od komercijalno dostupnog vina. Dobijeni rezultati su ukazali da umereno konzumiranje crvenog vina sorte merlo sa specifičnim geografskim poreklom predstavlja dobar izvor bioaktivnih komponenata sa pozitivnim biološkim efektom, što je od posebne važnosti za klonska sortna vina.",
publisher = "Beograd : Srpsko biološko društvo",
journal = "3. kongres biologa Srbije : osnovna i primenjena istraživanja, metodika nastave : knjiga sažetaka",
title = "Citotoksično dejstvo crvenog vina na tumorske ćelije u in vitro uslovima",
pages = "321-321",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12592"
}

Đorđević, N., Todorović Vukotić, N., Korićanac, L., Žakula, J., Pejić, S., Tešević, V.,& Pajović, S. B.. (2022). Citotoksično dejstvo crvenog vina na tumorske ćelije u in vitro uslovima. in 3. kongres biologa Srbije : osnovna i primenjena istraživanja, metodika nastave : knjiga sažetaka
Beograd : Srpsko biološko društvo., 321-321.
https://hdl.handle.net/21.15107/rcub_vinar_12592

Đorđević N, Todorović Vukotić N, Korićanac L, Žakula J, Pejić S, Tešević V, Pajović SB. Citotoksično dejstvo crvenog vina na tumorske ćelije u in vitro uslovima. in 3. kongres biologa Srbije : osnovna i primenjena istraživanja, metodika nastave : knjiga sažetaka. 2022;:321-321.
https://hdl.handle.net/21.15107/rcub_vinar_12592 .

Đorđević, Neda, Todorović Vukotić, Nevena, Korićanac, Lela, Žakula, Jelena, Pejić, Snežana, Tešević, V., Pajović, Snežana B., "Citotoksično dejstvo crvenog vina na tumorske ćelije u in vitro uslovima" in 3. kongres biologa Srbije : osnovna i primenjena istraživanja, metodika nastave : knjiga sažetaka (2022):321-321,
https://hdl.handle.net/21.15107/rcub_vinar_12592 .

TY  - CONF
AU  - Đorđević, Neda
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Todorović Vukotić, Nevena
AU  - Pejić, Snežana
AU  - Tešević, V.
AU  - Pajović, Snežana B.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12583
AB  - Melanoma is responsible for 75% of all deaths from skin cancer. Its lethality arises from its rapid progression, easy metastasis and drug-resistance as well. Red wine is a natural product rich in polyphenolic compounds with potent anticancer activities. It seems that in cancer cells these compounds behave as prooxidants initiating reactive oxygen species mediated cellular DNA breakage and consequent cell death. The aim of this study was to investigate prooxidative activity of red wine samples (Merlot variety, commercial as well as VCR1 and VCR101 clonal wines) in melanoma A375 cells, through measuring the relationship of reduced and oxidized form of glutathione (GSH/GSSG) and comparison with the GSH/GSSG ratio in control (untreated melanoma cells). The data obtained showed that tested red wine samples decrease GSH/GSSG ratio in A375 cells compared to control (4.6 ± 0), with the largest decrease noticed in treatment with VCR101 wine (0.66 ± 0.05).
PB  - The Society of Physical Chemists of Serbia
C3  - 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume II, September 20-24
T1  - Examination of Prooxidative Activity of Red Wine in Melanoma Cells
SP  - 699
EP  - 702
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12583
ER  - 

@conference{
author = "Đorđević, Neda and Korićanac, Lela and Žakula, Jelena and Todorović Vukotić, Nevena and Pejić, Snežana and Tešević, V. and Pajović, Snežana B.",
year = "2021",
abstract = "Melanoma is responsible for 75% of all deaths from skin cancer. Its lethality arises from its rapid progression, easy metastasis and drug-resistance as well. Red wine is a natural product rich in polyphenolic compounds with potent anticancer activities. It seems that in cancer cells these compounds behave as prooxidants initiating reactive oxygen species mediated cellular DNA breakage and consequent cell death. The aim of this study was to investigate prooxidative activity of red wine samples (Merlot variety, commercial as well as VCR1 and VCR101 clonal wines) in melanoma A375 cells, through measuring the relationship of reduced and oxidized form of glutathione (GSH/GSSG) and comparison with the GSH/GSSG ratio in control (untreated melanoma cells). The data obtained showed that tested red wine samples decrease GSH/GSSG ratio in A375 cells compared to control (4.6 ± 0), with the largest decrease noticed in treatment with VCR101 wine (0.66 ± 0.05).",
publisher = "The Society of Physical Chemists of Serbia",
journal = "15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume II, September 20-24",
title = "Examination of Prooxidative Activity of Red Wine in Melanoma Cells",
pages = "699-702",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12583"
}

Đorđević, N., Korićanac, L., Žakula, J., Todorović Vukotić, N., Pejić, S., Tešević, V.,& Pajović, S. B.. (2021). Examination of Prooxidative Activity of Red Wine in Melanoma Cells. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume II, September 20-24
The Society of Physical Chemists of Serbia., 699-702.
https://hdl.handle.net/21.15107/rcub_vinar_12583

Đorđević N, Korićanac L, Žakula J, Todorović Vukotić N, Pejić S, Tešević V, Pajović SB. Examination of Prooxidative Activity of Red Wine in Melanoma Cells. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume II, September 20-24. 2021;:699-702.
https://hdl.handle.net/21.15107/rcub_vinar_12583 .

Đorđević, Neda, Korićanac, Lela, Žakula, Jelena, Todorović Vukotić, Nevena, Pejić, Snežana, Tešević, V., Pajović, Snežana B., "Examination of Prooxidative Activity of Red Wine in Melanoma Cells" in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume II, September 20-24 (2021):699-702,
https://hdl.handle.net/21.15107/rcub_vinar_12583 .

TY  - CONF
AU  - Todorović, Ana
AU  - Drakulić, Dunja
AU  - Gavrilović, Ljubica
AU  - Stojiljković, Vesna
AU  - Popović, Nataša M.
AU  - Đorđević, Neda
AU  - Todorović Vukotić, Nevena
AU  - Miletić, J.
AU  - Pejić, Snežana
AU  - Pajović, Snežana B.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12580
AB  - The literature emphasizes the involvement of oxidative stress in the etiopathogenesis of many uterine diseases. Antioxidant system (AOS) represents the protective mechanism used by cells to neutralize overproduced reactive oxygen species (ROS) and prevent oxidative stress. We have previously shown that in gynecological patients with various diagnoses, the reproductive and other factors may be associated with antioxidant capacity and the ability to defend against oxidative damage. In this study, we examined the changes in expression of antioxidant enzymes (AOE): superoxide dismutases (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in the blood of women with endometrial hyperplasia. Our results indicate that hyperplasia induces perturbance in oxidative balance, particularly in glutathione redox cycle enzymes.
PB  - The Society of Physical Chemists of Serbia
C3  - 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume I, September 20-24
T1  - Antioxidant Enzymes in Blood of Women With Uterine Hyperplasia
SP  - 308
EP  - 311
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12580
ER  - 

@conference{
author = "Todorović, Ana and Drakulić, Dunja and Gavrilović, Ljubica and Stojiljković, Vesna and Popović, Nataša M. and Đorđević, Neda and Todorović Vukotić, Nevena and Miletić, J. and Pejić, Snežana and Pajović, Snežana B.",
year = "2021",
abstract = "The literature emphasizes the involvement of oxidative stress in the etiopathogenesis of many uterine diseases. Antioxidant system (AOS) represents the protective mechanism used by cells to neutralize overproduced reactive oxygen species (ROS) and prevent oxidative stress. We have previously shown that in gynecological patients with various diagnoses, the reproductive and other factors may be associated with antioxidant capacity and the ability to defend against oxidative damage. In this study, we examined the changes in expression of antioxidant enzymes (AOE): superoxide dismutases (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in the blood of women with endometrial hyperplasia. Our results indicate that hyperplasia induces perturbance in oxidative balance, particularly in glutathione redox cycle enzymes.",
publisher = "The Society of Physical Chemists of Serbia",
journal = "15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume I, September 20-24",
title = "Antioxidant Enzymes in Blood of Women With Uterine Hyperplasia",
pages = "308-311",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12580"
}

Todorović, A., Drakulić, D., Gavrilović, L., Stojiljković, V., Popović, N. M., Đorđević, N., Todorović Vukotić, N., Miletić, J., Pejić, S.,& Pajović, S. B.. (2021). Antioxidant Enzymes in Blood of Women With Uterine Hyperplasia. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume I, September 20-24
The Society of Physical Chemists of Serbia., 308-311.
https://hdl.handle.net/21.15107/rcub_vinar_12580

Todorović A, Drakulić D, Gavrilović L, Stojiljković V, Popović NM, Đorđević N, Todorović Vukotić N, Miletić J, Pejić S, Pajović SB. Antioxidant Enzymes in Blood of Women With Uterine Hyperplasia. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume I, September 20-24. 2021;:308-311.
https://hdl.handle.net/21.15107/rcub_vinar_12580 .

Todorović, Ana, Drakulić, Dunja, Gavrilović, Ljubica, Stojiljković, Vesna, Popović, Nataša M., Đorđević, Neda, Todorović Vukotić, Nevena, Miletić, J., Pejić, Snežana, Pajović, Snežana B., "Antioxidant Enzymes in Blood of Women With Uterine Hyperplasia" in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 : proceedings, Volume I, September 20-24 (2021):308-311,
https://hdl.handle.net/21.15107/rcub_vinar_12580 .

TY  - JOUR
AU  - Todorović Vukotić, Nevena
AU  - Đorđević, Jelena
AU  - Pejić, Snežana
AU  - Đorđević, Neda O.
AU  - Pajović, Snežana B.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9078
AB  - Drug-induced liver injury (DILI) is a serious health burden. It has diverse clinical presentations that can escalate to acute liver failure. The worldwide increase in the use of psychotropic drugs, their long-term use on a daily basis, common comorbidities of psychiatric and metabolic disorders, and polypharmacy in psychiatric patients increase the incidence of psychotropics-induced DILI. During the last 2 decades, hepatotoxicity of various antidepressants (ADs) and antipsychotics (APs) received much attention. Comprehensive review and discussion of accumulated literature data concerning this issue are performed in this study, as hepatotoxic effects of most commonly prescribed ADs and APs are classified, described, and discussed. The review focuses on ADs and APs characterized by the risk of causing liver damage and highlights the ones found to cause life-threatening or severe DILI cases. In parallel, an overview of hepatic oxidative stress, inflammation, and steatosis underlying DILI is provided, followed by extensive review and discussion of the pathophysiology of AD- and AP-induced DILI revealed in case reports, and animal and in vitro studies. The consequences of some ADs and APs ability to affect drug-metabolizing enzymes and therefore provoke drug–drug interactions are also addressed. Continuous collecting of data on drugs, mechanisms, and risk factors for DILI, as well as critical data reviewing, is crucial for easier DILI diagnosis and more efficient risk assessment of AD- and AP-induced DILI. Higher awareness of ADs and APs hepatotoxicity is the prerequisite for their safe use and optimal dosing. © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.
T2  - Archives of Toxicology
T1  - Antidepressants- and antipsychotics-induced hepatotoxicity
VL  - 95
IS  - 3
SP  - 767
EP  - 789
DO  - 10.1007/s00204-020-02963-4
ER  - 

@article{
author = "Todorović Vukotić, Nevena and Đorđević, Jelena and Pejić, Snežana and Đorđević, Neda O. and Pajović, Snežana B.",
year = "2021",
abstract = "Drug-induced liver injury (DILI) is a serious health burden. It has diverse clinical presentations that can escalate to acute liver failure. The worldwide increase in the use of psychotropic drugs, their long-term use on a daily basis, common comorbidities of psychiatric and metabolic disorders, and polypharmacy in psychiatric patients increase the incidence of psychotropics-induced DILI. During the last 2 decades, hepatotoxicity of various antidepressants (ADs) and antipsychotics (APs) received much attention. Comprehensive review and discussion of accumulated literature data concerning this issue are performed in this study, as hepatotoxic effects of most commonly prescribed ADs and APs are classified, described, and discussed. The review focuses on ADs and APs characterized by the risk of causing liver damage and highlights the ones found to cause life-threatening or severe DILI cases. In parallel, an overview of hepatic oxidative stress, inflammation, and steatosis underlying DILI is provided, followed by extensive review and discussion of the pathophysiology of AD- and AP-induced DILI revealed in case reports, and animal and in vitro studies. The consequences of some ADs and APs ability to affect drug-metabolizing enzymes and therefore provoke drug–drug interactions are also addressed. Continuous collecting of data on drugs, mechanisms, and risk factors for DILI, as well as critical data reviewing, is crucial for easier DILI diagnosis and more efficient risk assessment of AD- and AP-induced DILI. Higher awareness of ADs and APs hepatotoxicity is the prerequisite for their safe use and optimal dosing. © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.",
journal = "Archives of Toxicology",
title = "Antidepressants- and antipsychotics-induced hepatotoxicity",
volume = "95",
number = "3",
pages = "767-789",
doi = "10.1007/s00204-020-02963-4"
}

Todorović Vukotić, N., Đorđević, J., Pejić, S., Đorđević, N. O.,& Pajović, S. B.. (2021). Antidepressants- and antipsychotics-induced hepatotoxicity. in Archives of Toxicology, 95(3), 767-789.
https://doi.org/10.1007/s00204-020-02963-4

Todorović Vukotić N, Đorđević J, Pejić S, Đorđević NO, Pajović SB. Antidepressants- and antipsychotics-induced hepatotoxicity. in Archives of Toxicology. 2021;95(3):767-789.
doi:10.1007/s00204-020-02963-4 .

Todorović Vukotić, Nevena, Đorđević, Jelena, Pejić, Snežana, Đorđević, Neda O., Pajović, Snežana B., "Antidepressants- and antipsychotics-induced hepatotoxicity" in Archives of Toxicology, 95, no. 3 (2021):767-789,
https://doi.org/10.1007/s00204-020-02963-4 . .

TY  - JOUR
AU  - Đurašević, Siniša
AU  - Pejić, Snežana
AU  - Grigorov, Ilijana
AU  - Nikolić, Gorana
AU  - Mitić-Ćulafić, Dragana
AU  - Dragićević, Milan
AU  - Đorđević, Jelena
AU  - Todorović Vukotić, Nevena
AU  - Đorđević, Neda O.
AU  - Todorović, Ana
AU  - Drakulić, Dunja R.
AU  - Veljković, Filip M.
AU  - Pajović, Snežana B.
AU  - Todorović, Zoran
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9831
AB  - Thioacetamide (TAA) is widely used to study liver toxicity accompanied by oxidative stress, inflammation, cell necrosis, fibrosis, cholestasis, and hepatocellular carcinoma. As an efficient free radical’s scavenger, C60 fullerene is considered a potential liver-protective agent in chemically-induced liver injury. In the present work, we examined the hepatoprotective effects of two C60 doses dissolved in virgin olive oil against TAA-induced hepatotoxicity in rats. We showed that TAA-induced increase in liver oxidative stress, judged by the changes in the activities of SOD, CAT, GPx, GR, GST, the content of GSH and 4-HNE, and expression of HO-1, MnSOD, and CuZnSOD, was more effectively ameliorated with a lower C60 dose. Improvement in liver antioxidative status caused by C60 was accompanied by a decrease in liver HMGB1 expression and an increase in nuclear Nrf2/NF-κB p65 ratio, suggesting a reduction in inflammation, necrosis and fibrosis. These results were in accordance with liver histology analysis, liver comet assay, and changes in serum levels of ALT, AST, and AP. The changes observed in gut microbiome support detrimental effects of TAA and hepatoprotective effects of low C60 dose. Less protective effects of a higher C60 dose could be a consequence of its enhanced aggregation and related pro-oxidant role.
T2  - Antioxidants
T1  - Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes
VL  - 10
IS  - 6
SP  - 911
DO  - 10.3390/antiox10060911
ER  - 

@article{
author = "Đurašević, Siniša and Pejić, Snežana and Grigorov, Ilijana and Nikolić, Gorana and Mitić-Ćulafić, Dragana and Dragićević, Milan and Đorđević, Jelena and Todorović Vukotić, Nevena and Đorđević, Neda O. and Todorović, Ana and Drakulić, Dunja R. and Veljković, Filip M. and Pajović, Snežana B. and Todorović, Zoran",
year = "2021",
abstract = "Thioacetamide (TAA) is widely used to study liver toxicity accompanied by oxidative stress, inflammation, cell necrosis, fibrosis, cholestasis, and hepatocellular carcinoma. As an efficient free radical’s scavenger, C60 fullerene is considered a potential liver-protective agent in chemically-induced liver injury. In the present work, we examined the hepatoprotective effects of two C60 doses dissolved in virgin olive oil against TAA-induced hepatotoxicity in rats. We showed that TAA-induced increase in liver oxidative stress, judged by the changes in the activities of SOD, CAT, GPx, GR, GST, the content of GSH and 4-HNE, and expression of HO-1, MnSOD, and CuZnSOD, was more effectively ameliorated with a lower C60 dose. Improvement in liver antioxidative status caused by C60 was accompanied by a decrease in liver HMGB1 expression and an increase in nuclear Nrf2/NF-κB p65 ratio, suggesting a reduction in inflammation, necrosis and fibrosis. These results were in accordance with liver histology analysis, liver comet assay, and changes in serum levels of ALT, AST, and AP. The changes observed in gut microbiome support detrimental effects of TAA and hepatoprotective effects of low C60 dose. Less protective effects of a higher C60 dose could be a consequence of its enhanced aggregation and related pro-oxidant role.",
journal = "Antioxidants",
title = "Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes",
volume = "10",
number = "6",
pages = "911",
doi = "10.3390/antiox10060911"
}

Đurašević, S., Pejić, S., Grigorov, I., Nikolić, G., Mitić-Ćulafić, D., Dragićević, M., Đorđević, J., Todorović Vukotić, N., Đorđević, N. O., Todorović, A., Drakulić, D. R., Veljković, F. M., Pajović, S. B.,& Todorović, Z.. (2021). Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes. in Antioxidants, 10(6), 911.
https://doi.org/10.3390/antiox10060911

Đurašević S, Pejić S, Grigorov I, Nikolić G, Mitić-Ćulafić D, Dragićević M, Đorđević J, Todorović Vukotić N, Đorđević NO, Todorović A, Drakulić DR, Veljković FM, Pajović SB, Todorović Z. Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes. in Antioxidants. 2021;10(6):911.
doi:10.3390/antiox10060911 .

Đurašević, Siniša, Pejić, Snežana, Grigorov, Ilijana, Nikolić, Gorana, Mitić-Ćulafić, Dragana, Dragićević, Milan, Đorđević, Jelena, Todorović Vukotić, Nevena, Đorđević, Neda O., Todorović, Ana, Drakulić, Dunja R., Veljković, Filip M., Pajović, Snežana B., Todorović, Zoran, "Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes" in Antioxidants, 10, no. 6 (2021):911,
https://doi.org/10.3390/antiox10060911 . .

TY  - JOUR
AU  - Todorović, Nevena
AU  - Mićić, Bojana
AU  - Schwirtlich, Marija
AU  - Stevanović, Milena J.
AU  - Filipović, Dragana
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0306452218307322
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7982
AB  - Dysregulation of GABAergic system is becoming increasingly associated with depression, psychiatric disorder that imposes severe clinical, social and economic burden. Special attention is paid to the fast-spiking parvalbumin-positive (PV+) interneurons, GABAergic neurons which are highly susceptible to redox dysregulation and oxidative stress and implicated in a variety of psychiatric diseases. Here we analyzed the number of PV+ and cleaved caspase-3-positive (CC3+) cells in the rat medial prefrontal cortical (mPFC) subregions following chronic social isolation (CSIS), an animal model of depression and schizophrenia. Also, we examined potential protective effects of antidepressant fluoxetine (FLX) and atypical antipsychotic clozapine (CLZ) on the number of these cells in mPFC subregions, when applied parallel with CSIS in doses that correspond to therapeutically effective ones in patients. Immunofluorescence analysis revealed decreased number of PV+ cells in cingulate cortex area 1, prelimbic area (PrL), infralimbic area (IL) and dorsal peduncular cortex of the mPFC in isolated rats, which coincided with depressive- and anxiety-like behaviors. In addition, CSIS-induced increase in the number of CC3+ cells was detected in aforementioned subregions of mPFC. Treatments with either FLX or CLZ prevented behavioral changes, decrease in PV+ and increase in CC3+ cell numbers in PrL and IL subregions in isolated rats. These results indicate the importance of intact GABAergic signaling in these areas for resistance against CSIS-induced behavioral changes, as well as subregion-specific protective effects of FLX and CLZ in mPFC of CSIS rats. © 2018 IBRO
T2  - Neuroscience
T1  - Subregion-specific Protective Effects of Fluoxetine and Clozapine on Parvalbumin Expression in Medial Prefrontal Cortex of Chronically Isolated Rats
VL  - 396
SP  - 24
EP  - 35
DO  - 10.1016/j.neuroscience.2018.11.008
ER  - 

@article{
author = "Todorović, Nevena and Mićić, Bojana and Schwirtlich, Marija and Stevanović, Milena J. and Filipović, Dragana",
year = "2019",
abstract = "Dysregulation of GABAergic system is becoming increasingly associated with depression, psychiatric disorder that imposes severe clinical, social and economic burden. Special attention is paid to the fast-spiking parvalbumin-positive (PV+) interneurons, GABAergic neurons which are highly susceptible to redox dysregulation and oxidative stress and implicated in a variety of psychiatric diseases. Here we analyzed the number of PV+ and cleaved caspase-3-positive (CC3+) cells in the rat medial prefrontal cortical (mPFC) subregions following chronic social isolation (CSIS), an animal model of depression and schizophrenia. Also, we examined potential protective effects of antidepressant fluoxetine (FLX) and atypical antipsychotic clozapine (CLZ) on the number of these cells in mPFC subregions, when applied parallel with CSIS in doses that correspond to therapeutically effective ones in patients. Immunofluorescence analysis revealed decreased number of PV+ cells in cingulate cortex area 1, prelimbic area (PrL), infralimbic area (IL) and dorsal peduncular cortex of the mPFC in isolated rats, which coincided with depressive- and anxiety-like behaviors. In addition, CSIS-induced increase in the number of CC3+ cells was detected in aforementioned subregions of mPFC. Treatments with either FLX or CLZ prevented behavioral changes, decrease in PV+ and increase in CC3+ cell numbers in PrL and IL subregions in isolated rats. These results indicate the importance of intact GABAergic signaling in these areas for resistance against CSIS-induced behavioral changes, as well as subregion-specific protective effects of FLX and CLZ in mPFC of CSIS rats. © 2018 IBRO",
journal = "Neuroscience",
title = "Subregion-specific Protective Effects of Fluoxetine and Clozapine on Parvalbumin Expression in Medial Prefrontal Cortex of Chronically Isolated Rats",
volume = "396",
pages = "24-35",
doi = "10.1016/j.neuroscience.2018.11.008"
}

Todorović, N., Mićić, B., Schwirtlich, M., Stevanović, M. J.,& Filipović, D.. (2019). Subregion-specific Protective Effects of Fluoxetine and Clozapine on Parvalbumin Expression in Medial Prefrontal Cortex of Chronically Isolated Rats. in Neuroscience, 396, 24-35.
https://doi.org/10.1016/j.neuroscience.2018.11.008

Todorović N, Mićić B, Schwirtlich M, Stevanović MJ, Filipović D. Subregion-specific Protective Effects of Fluoxetine and Clozapine on Parvalbumin Expression in Medial Prefrontal Cortex of Chronically Isolated Rats. in Neuroscience. 2019;396:24-35.
doi:10.1016/j.neuroscience.2018.11.008 .

Todorović, Nevena, Mićić, Bojana, Schwirtlich, Marija, Stevanović, Milena J., Filipović, Dragana, "Subregion-specific Protective Effects of Fluoxetine and Clozapine on Parvalbumin Expression in Medial Prefrontal Cortex of Chronically Isolated Rats" in Neuroscience, 396 (2019):24-35,
https://doi.org/10.1016/j.neuroscience.2018.11.008 . .

TY  - JOUR
AU  - Đorđević, Neda O.
AU  - Todorović, Nevena
AU  - Novaković, Irena T.
AU  - Pezo, Lato
AU  - Pejin, Boris
AU  - Maraš, Vesna
AU  - Tešević, Vele V.
AU  - Pajović, Snežana B.
PY  - 2018
UR  - http://www.mdpi.com/1420-3049/23/8/1971
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7858
AB  - Screens of antioxidant activity (AA) of various natural products have been a focus of the research community worldwide. This work aimed to differentiate selected samples of Merlot wines originated from Montenegro, with regard to phenolic profile and antioxidant capacity studied by survival rate, total sulfhydryl groups and activities of glutathione peroxidase (GPx), glutathione reductase and catalase in H2O2–stressed Saccharomyces cerevisiae cells. In this study, DPPH assay was also performed. Higher total phenolic content leads to an enhanced AA under both conditions. The same trend was observed for catechin and gallic acid, the most abundant phenolics in the examined wine samples. Finally, the findings of an Artificial Neural Network (ANN) model were in a good agreement (r2 = 0.978) with the experimental data. All tested samples exhibited a protective effect in H2O2–stressed yeast cells. Pre-treatment with examined wines increased survival in H2O2–stressed cells and shifted antioxidative defense towards GPx–mediated defense. Finally, sensitivity analysis of obtained ANN model highlights the complexity of the impact that variations in the concentrations of specific phenolic components have on the antioxidant defense system.
T2  - Molecules
T1  - Antioxidant Activity of Selected Polyphenolics in Yeast Cells: The Case Study of Montenegrin Merlot Wine
VL  - 23
IS  - 8
SP  - 1971
DO  - 10.3390/molecules23081971
ER  - 

@article{
author = "Đorđević, Neda O. and Todorović, Nevena and Novaković, Irena T. and Pezo, Lato and Pejin, Boris and Maraš, Vesna and Tešević, Vele V. and Pajović, Snežana B.",
year = "2018",
abstract = "Screens of antioxidant activity (AA) of various natural products have been a focus of the research community worldwide. This work aimed to differentiate selected samples of Merlot wines originated from Montenegro, with regard to phenolic profile and antioxidant capacity studied by survival rate, total sulfhydryl groups and activities of glutathione peroxidase (GPx), glutathione reductase and catalase in H2O2–stressed Saccharomyces cerevisiae cells. In this study, DPPH assay was also performed. Higher total phenolic content leads to an enhanced AA under both conditions. The same trend was observed for catechin and gallic acid, the most abundant phenolics in the examined wine samples. Finally, the findings of an Artificial Neural Network (ANN) model were in a good agreement (r2 = 0.978) with the experimental data. All tested samples exhibited a protective effect in H2O2–stressed yeast cells. Pre-treatment with examined wines increased survival in H2O2–stressed cells and shifted antioxidative defense towards GPx–mediated defense. Finally, sensitivity analysis of obtained ANN model highlights the complexity of the impact that variations in the concentrations of specific phenolic components have on the antioxidant defense system.",
journal = "Molecules",
title = "Antioxidant Activity of Selected Polyphenolics in Yeast Cells: The Case Study of Montenegrin Merlot Wine",
volume = "23",
number = "8",
pages = "1971",
doi = "10.3390/molecules23081971"
}

Đorđević, N. O., Todorović, N., Novaković, I. T., Pezo, L., Pejin, B., Maraš, V., Tešević, V. V.,& Pajović, S. B.. (2018). Antioxidant Activity of Selected Polyphenolics in Yeast Cells: The Case Study of Montenegrin Merlot Wine. in Molecules, 23(8), 1971.
https://doi.org/10.3390/molecules23081971

Đorđević NO, Todorović N, Novaković IT, Pezo L, Pejin B, Maraš V, Tešević VV, Pajović SB. Antioxidant Activity of Selected Polyphenolics in Yeast Cells: The Case Study of Montenegrin Merlot Wine. in Molecules. 2018;23(8):1971.
doi:10.3390/molecules23081971 .

Đorđević, Neda O., Todorović, Nevena, Novaković, Irena T., Pezo, Lato, Pejin, Boris, Maraš, Vesna, Tešević, Vele V., Pajović, Snežana B., "Antioxidant Activity of Selected Polyphenolics in Yeast Cells: The Case Study of Montenegrin Merlot Wine" in Molecules, 23, no. 8 (2018):1971,
https://doi.org/10.3390/molecules23081971 . .

TY  - JOUR
AU  - Filipović, Dragana
AU  - Todorović, Nevena
AU  - Bernardi, Rick E.
AU  - Gass, Peter
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1392
AB  - Various stressors may disrupt the redox homeostasis of an organism by causing oxidative and nitrosative stress that may activate stressor-specific pathways and provoke specific responses. Chronic social isolation (CSIS) represents a mild chronic stress that evokes a variety of neurobehavioral changes in rats similar to those observed in people with psychiatric disorders, including depression. Most rodent studies have focused on the effect of social isolation during weaning or adolescence, while its effect in adult rats has not been extensively examined. In this review, we discuss the current knowledge regarding the involvement of oxidative/nitrosative stress pathways in the prefrontal cortex and hippocampus of adult male rats exposed to CSIS, focusing on hypothalamic-pituitary-adrenocortical (HPA) axis activity, behavior parameters, antioxidative defense systems, stress signaling mediated by nuclear factor-kappa B (NF-kappa B), and mitochondria-related proapoptotic signaling. Although increased concentrations of corticosterone (CORT) have been shown to induce oxidative and nitrosative stress, we suggest a mechanism underlying the glucocorticoid paradox whereby a state of oxidative/nitrosative stress may exist under basal CORT levels. This review also highlights the differential susceptibility of prefrontal cortex and hippocampus to oxidative stress following CSIS and suggests a possible cellular pathway of stress tolerance that preserves the hippocampus from molecular damage and apoptosis. The differential regulation of the transcriptional factor NF-kappa B, and the enzymes inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) following CSIS may be one functional difference between the response of the prefrontal cortex and hippocampus, thus identifying potentially relevant targets for antidepressant treatment.
T2  - Brain Structure and Function
T1  - Oxidative and nitrosative stress pathways in the brain of socially isolated adult male rats demonstrating depressive- and anxiety-like symptoms
VL  - 222
IS  - 1
SP  - 1
EP  - 20
DO  - 10.1007/s00429-016-1218-9
ER  - 

@article{
author = "Filipović, Dragana and Todorović, Nevena and Bernardi, Rick E. and Gass, Peter",
year = "2017",
abstract = "Various stressors may disrupt the redox homeostasis of an organism by causing oxidative and nitrosative stress that may activate stressor-specific pathways and provoke specific responses. Chronic social isolation (CSIS) represents a mild chronic stress that evokes a variety of neurobehavioral changes in rats similar to those observed in people with psychiatric disorders, including depression. Most rodent studies have focused on the effect of social isolation during weaning or adolescence, while its effect in adult rats has not been extensively examined. In this review, we discuss the current knowledge regarding the involvement of oxidative/nitrosative stress pathways in the prefrontal cortex and hippocampus of adult male rats exposed to CSIS, focusing on hypothalamic-pituitary-adrenocortical (HPA) axis activity, behavior parameters, antioxidative defense systems, stress signaling mediated by nuclear factor-kappa B (NF-kappa B), and mitochondria-related proapoptotic signaling. Although increased concentrations of corticosterone (CORT) have been shown to induce oxidative and nitrosative stress, we suggest a mechanism underlying the glucocorticoid paradox whereby a state of oxidative/nitrosative stress may exist under basal CORT levels. This review also highlights the differential susceptibility of prefrontal cortex and hippocampus to oxidative stress following CSIS and suggests a possible cellular pathway of stress tolerance that preserves the hippocampus from molecular damage and apoptosis. The differential regulation of the transcriptional factor NF-kappa B, and the enzymes inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) following CSIS may be one functional difference between the response of the prefrontal cortex and hippocampus, thus identifying potentially relevant targets for antidepressant treatment.",
journal = "Brain Structure and Function",
title = "Oxidative and nitrosative stress pathways in the brain of socially isolated adult male rats demonstrating depressive- and anxiety-like symptoms",
volume = "222",
number = "1",
pages = "1-20",
doi = "10.1007/s00429-016-1218-9"
}

Filipović, D., Todorović, N., Bernardi, R. E.,& Gass, P.. (2017). Oxidative and nitrosative stress pathways in the brain of socially isolated adult male rats demonstrating depressive- and anxiety-like symptoms. in Brain Structure and Function, 222(1), 1-20.
https://doi.org/10.1007/s00429-016-1218-9

Filipović D, Todorović N, Bernardi RE, Gass P. Oxidative and nitrosative stress pathways in the brain of socially isolated adult male rats demonstrating depressive- and anxiety-like symptoms. in Brain Structure and Function. 2017;222(1):1-20.
doi:10.1007/s00429-016-1218-9 .

Filipović, Dragana, Todorović, Nevena, Bernardi, Rick E., Gass, Peter, "Oxidative and nitrosative stress pathways in the brain of socially isolated adult male rats demonstrating depressive- and anxiety-like symptoms" in Brain Structure and Function, 222, no. 1 (2017):1-20,
https://doi.org/10.1007/s00429-016-1218-9 . .

TY  - JOUR
AU  - Todorović, Nevena
AU  - Filipović, Dragana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1875
AB  - Brain oxidative stress and neuroinflammation are implicated in psychiatric disorders. Thus, it is important to investigate the effects of individual psychotropic agents on antioxidative defense and proinflammatory mediators in brain regions associated with these disorders. Psychosocial stress is recognized as a threat to mental health, and the hippocampus is a primary target of stress-related damage. Chronic social isolation (CSIS) is a mild psychosocial stress used to model the pathophysiology of depression. We examined the antioxidative and anti-inflammatory potential of the antidepressant fluoxetine (FLX) and atypical antipsychotic clozapine (CLZ) in the hippocampus in the CSIS model of depression. We measured the effects of FLX and CLZ on depressive- and anxiety-like behaviors in non-stressed rats and rats exposed to 21d of CSIS. We further evaluated the content of reduced glutathione (GSH), the protein expression and activity of the GSH-related enzymes, the subcellular localization of nuclear factor-kappa B (NF-kappa B)and protein levels of proinflammatory mediators cyclooxygenase-2 (COX-2), interleukin-lbeta (IL-1 beta), and tumor necrosis factor-alpha (TNF-alpha) in these groups of rats. CSIS resulted in an increase in depressive- and anxiety-like behaviors that corresponded with compromised glutathione peroxidase (GPx)-mediated antioxidative defense and increased TNF-alpha, but not with changes in NF-kappa B, IL-1 beta and COX-2 levels. FLX and CLZ, applied during CSIS, prevented the behavioral changes associated with CSIS, and inhibited the increase in TNF-alpha, but did not affect GPx-mediated antioxidative defense. Furthermore, both drugs decreased hippocampal GPx activity when applied to non-stressed rats. These results emphasize the significance of hippocampal TNF-alpha-mediated proinflammmatory signaling in the pathophysiology of depressive symptoms and the importance of the anti-inflammatory action of both FLX and CLZ in the prevention of these symptoms.
T2  - Pharmacology Biochemistry and Behavior
T1  - The antidepressant- and anxiolytic-like effects of fluoxetine and clozapine in chronically isolated rats involve inhibition of hippocampal TNF-alpha
VL  - 163
SP  - 57
EP  - 65
DO  - 10.1016/j.pbb.2017.10.006
ER  - 

@article{
author = "Todorović, Nevena and Filipović, Dragana",
year = "2017",
abstract = "Brain oxidative stress and neuroinflammation are implicated in psychiatric disorders. Thus, it is important to investigate the effects of individual psychotropic agents on antioxidative defense and proinflammatory mediators in brain regions associated with these disorders. Psychosocial stress is recognized as a threat to mental health, and the hippocampus is a primary target of stress-related damage. Chronic social isolation (CSIS) is a mild psychosocial stress used to model the pathophysiology of depression. We examined the antioxidative and anti-inflammatory potential of the antidepressant fluoxetine (FLX) and atypical antipsychotic clozapine (CLZ) in the hippocampus in the CSIS model of depression. We measured the effects of FLX and CLZ on depressive- and anxiety-like behaviors in non-stressed rats and rats exposed to 21d of CSIS. We further evaluated the content of reduced glutathione (GSH), the protein expression and activity of the GSH-related enzymes, the subcellular localization of nuclear factor-kappa B (NF-kappa B)and protein levels of proinflammatory mediators cyclooxygenase-2 (COX-2), interleukin-lbeta (IL-1 beta), and tumor necrosis factor-alpha (TNF-alpha) in these groups of rats. CSIS resulted in an increase in depressive- and anxiety-like behaviors that corresponded with compromised glutathione peroxidase (GPx)-mediated antioxidative defense and increased TNF-alpha, but not with changes in NF-kappa B, IL-1 beta and COX-2 levels. FLX and CLZ, applied during CSIS, prevented the behavioral changes associated with CSIS, and inhibited the increase in TNF-alpha, but did not affect GPx-mediated antioxidative defense. Furthermore, both drugs decreased hippocampal GPx activity when applied to non-stressed rats. These results emphasize the significance of hippocampal TNF-alpha-mediated proinflammmatory signaling in the pathophysiology of depressive symptoms and the importance of the anti-inflammatory action of both FLX and CLZ in the prevention of these symptoms.",
journal = "Pharmacology Biochemistry and Behavior",
title = "The antidepressant- and anxiolytic-like effects of fluoxetine and clozapine in chronically isolated rats involve inhibition of hippocampal TNF-alpha",
volume = "163",
pages = "57-65",
doi = "10.1016/j.pbb.2017.10.006"
}

Todorović, N.,& Filipović, D.. (2017). The antidepressant- and anxiolytic-like effects of fluoxetine and clozapine in chronically isolated rats involve inhibition of hippocampal TNF-alpha. in Pharmacology Biochemistry and Behavior, 163, 57-65.
https://doi.org/10.1016/j.pbb.2017.10.006

Todorović N, Filipović D. The antidepressant- and anxiolytic-like effects of fluoxetine and clozapine in chronically isolated rats involve inhibition of hippocampal TNF-alpha. in Pharmacology Biochemistry and Behavior. 2017;163:57-65.
doi:10.1016/j.pbb.2017.10.006 .

Todorović, Nevena, Filipović, Dragana, "The antidepressant- and anxiolytic-like effects of fluoxetine and clozapine in chronically isolated rats involve inhibition of hippocampal TNF-alpha" in Pharmacology Biochemistry and Behavior, 163 (2017):57-65,
https://doi.org/10.1016/j.pbb.2017.10.006 . .

TY  - JOUR
AU  - Todorović, Nevena
AU  - Filipović, Dragana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1613
AB  - Chronic psychosocial stress modulates brain antioxidant systems and causes neuroinflammation that plays a role in the pathophysiology of depression. Although the antidepressant fluoxetine (FLX) represents the first-line treatment for depression and the atypical antipsychotic clozapine (CLZ) is considered as a second-line treatment for psychotic disorders, the downstream mechanisms of action of these treatments, beyond serotonergic or dopaminergic signaling, remain elusive. We examined behavioral changes, glutathione (GSH)-dependent defense and levels of proinflammatory mediators in the prefrontal cortex (PFC) of adult male Wistar rats exposed to 21 days of chronic social isolation (CSIS). We also tested the ability of FLX (15 mg/kg/day) or CLZ (20 mg/kg/day), applied during CSIS, to prevent stress-induced changes. CSIS caused depressive-and anxiety-like behaviors, compromised GSH-dependent defense, and induced nuclear factor-kappa B (NF-kappa B) activation with a concomitant increase in cytosolic levels of proinflammatory mediators cyclooxigenase-2, interleukin-1beta and tumor necrosis factor-alpha in the PFC. NF-kappa B activation and proinflammatory response in the PFC were not found in CSIS rats treated with FLX or CLZ. In contrast, only FLX preserved GSH content in CSIS rats. CLZ not only failed to protect against CSIS-induced GSH depletion, but it diminished its levels when applied to non-stressed rats. In conclusion, prefrontal cortical GSH depletion and the proinflammatory response underlying depressive-and anxiety-like states induced by CSIS were prevented by FLX. The protective effect of CLZ, which was equally effective as FLX on the behavioral level, was limited to proinflammatory components. Hence, different mechanisms underlie the protective effects of these two drugs in CSIS rats. (C) 2017 IBRO. Published by Elsevier Ltd. All rights reserved.
T2  - Neuroscience
T1  - Prefrontal Cortical Glutathione-Dependent Defense and Proinflammatory Mediators in Chronically Isolated Rats: Modulation By Fluoxetine Or Clozapine
VL  - 355
SP  - 49
EP  - 60
DO  - 10.1016/j.neuroscience.2017.04.044
ER  - 

@article{
author = "Todorović, Nevena and Filipović, Dragana",
year = "2017",
abstract = "Chronic psychosocial stress modulates brain antioxidant systems and causes neuroinflammation that plays a role in the pathophysiology of depression. Although the antidepressant fluoxetine (FLX) represents the first-line treatment for depression and the atypical antipsychotic clozapine (CLZ) is considered as a second-line treatment for psychotic disorders, the downstream mechanisms of action of these treatments, beyond serotonergic or dopaminergic signaling, remain elusive. We examined behavioral changes, glutathione (GSH)-dependent defense and levels of proinflammatory mediators in the prefrontal cortex (PFC) of adult male Wistar rats exposed to 21 days of chronic social isolation (CSIS). We also tested the ability of FLX (15 mg/kg/day) or CLZ (20 mg/kg/day), applied during CSIS, to prevent stress-induced changes. CSIS caused depressive-and anxiety-like behaviors, compromised GSH-dependent defense, and induced nuclear factor-kappa B (NF-kappa B) activation with a concomitant increase in cytosolic levels of proinflammatory mediators cyclooxigenase-2, interleukin-1beta and tumor necrosis factor-alpha in the PFC. NF-kappa B activation and proinflammatory response in the PFC were not found in CSIS rats treated with FLX or CLZ. In contrast, only FLX preserved GSH content in CSIS rats. CLZ not only failed to protect against CSIS-induced GSH depletion, but it diminished its levels when applied to non-stressed rats. In conclusion, prefrontal cortical GSH depletion and the proinflammatory response underlying depressive-and anxiety-like states induced by CSIS were prevented by FLX. The protective effect of CLZ, which was equally effective as FLX on the behavioral level, was limited to proinflammatory components. Hence, different mechanisms underlie the protective effects of these two drugs in CSIS rats. (C) 2017 IBRO. Published by Elsevier Ltd. All rights reserved.",
journal = "Neuroscience",
title = "Prefrontal Cortical Glutathione-Dependent Defense and Proinflammatory Mediators in Chronically Isolated Rats: Modulation By Fluoxetine Or Clozapine",
volume = "355",
pages = "49-60",
doi = "10.1016/j.neuroscience.2017.04.044"
}

Todorović, N.,& Filipović, D.. (2017). Prefrontal Cortical Glutathione-Dependent Defense and Proinflammatory Mediators in Chronically Isolated Rats: Modulation By Fluoxetine Or Clozapine. in Neuroscience, 355, 49-60.
https://doi.org/10.1016/j.neuroscience.2017.04.044

Todorović N, Filipović D. Prefrontal Cortical Glutathione-Dependent Defense and Proinflammatory Mediators in Chronically Isolated Rats: Modulation By Fluoxetine Or Clozapine. in Neuroscience. 2017;355:49-60.
doi:10.1016/j.neuroscience.2017.04.044 .

Todorović, Nevena, Filipović, Dragana, "Prefrontal Cortical Glutathione-Dependent Defense and Proinflammatory Mediators in Chronically Isolated Rats: Modulation By Fluoxetine Or Clozapine" in Neuroscience, 355 (2017):49-60,
https://doi.org/10.1016/j.neuroscience.2017.04.044 . .

TY  - CONF
AU  - Perić, Ivana
AU  - Todorović, Nevena
AU  - Stanisavljević, Andrijana
AU  - Đorđević, Neda O.
AU  - Filipović, Dragana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9200
AB  - Exposure   of   an   organism   to   chronic   social   isolation   (CSIS)   causes modulation  of  antioxidant  defense  system  in  the  brain  which  has  been shown to have important role in depression. Fluoxetine (Flx) is the first-line treatment  for  depression;  however,  precise  mechanism  of  its  action  still remains  elusive.  The  aim  of  this  study  was  to  investigate  the  effect  of  3 weeks  of  Flx  treatment  on  malondialdehyde  (MDA)  level,  an  oxidative stress parameter as well as on the activities of GSH-dependent antioxidative enzymes in the hippocampus of rats exposed to 6 weeks of CSIS. Increased MDA content following  CSIS and Flx treatment  (controls or CSIS) of rats, suggests  on  hippocampal  oxidative  damage.  CSIS  induced  reduction  of hippocampal  glutathione-S-transferase  that  was  reversed  by  Flx  treatment, as  well  as  an  increase  in  glutathione  peroxidase/reductase  activities.  The present  study  contributes  to  our  understanding  of  the  mechanisms  that underlie the antidepressant activity of Flx in rats exposed to CSIS, an animal model of depression.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry
T1  - The effects of fluoxetine on hippocampal antioxidative defense in depressive-like rats
SP  - 435
EP  - 438
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9200
ER  - 

@conference{
author = "Perić, Ivana and Todorović, Nevena and Stanisavljević, Andrijana and Đorđević, Neda O. and Filipović, Dragana",
year = "2016",
abstract = "Exposure   of   an   organism   to   chronic   social   isolation   (CSIS)   causes modulation  of  antioxidant  defense  system  in  the  brain  which  has  been shown to have important role in depression. Fluoxetine (Flx) is the first-line treatment  for  depression;  however,  precise  mechanism  of  its  action  still remains  elusive.  The  aim  of  this  study  was  to  investigate  the  effect  of  3 weeks  of  Flx  treatment  on  malondialdehyde  (MDA)  level,  an  oxidative stress parameter as well as on the activities of GSH-dependent antioxidative enzymes in the hippocampus of rats exposed to 6 weeks of CSIS. Increased MDA content following  CSIS and Flx treatment  (controls or CSIS) of rats, suggests  on  hippocampal  oxidative  damage.  CSIS  induced  reduction  of hippocampal  glutathione-S-transferase  that  was  reversed  by  Flx  treatment, as  well  as  an  increase  in  glutathione  peroxidase/reductase  activities.  The present  study  contributes  to  our  understanding  of  the  mechanisms  that underlie the antidepressant activity of Flx in rats exposed to CSIS, an animal model of depression.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry",
title = "The effects of fluoxetine on hippocampal antioxidative defense in depressive-like rats",
pages = "435-438",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9200"
}

Perić, I., Todorović, N., Stanisavljević, A., Đorđević, N. O.,& Filipović, D.. (2016). The effects of fluoxetine on hippocampal antioxidative defense in depressive-like rats. in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 435-438.
https://hdl.handle.net/21.15107/rcub_vinar_9200

Perić I, Todorović N, Stanisavljević A, Đorđević NO, Filipović D. The effects of fluoxetine on hippocampal antioxidative defense in depressive-like rats. in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry. 2016;:435-438.
https://hdl.handle.net/21.15107/rcub_vinar_9200 .

Perić, Ivana, Todorović, Nevena, Stanisavljević, Andrijana, Đorđević, Neda O., Filipović, Dragana, "The effects of fluoxetine on hippocampal antioxidative defense in depressive-like rats" in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry (2016):435-438,
https://hdl.handle.net/21.15107/rcub_vinar_9200 .

TY  - CONF
AU  - Stanisavljević, Andrijana
AU  - Perić, Ivana
AU  - Todorović, Nevena
AU  - Đorđević, Neda O.
AU  - Filipović, Dragana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9203
AB  - Olanzapine  is  an  antipsychotic  proved  to  be  effective  in stress  associated psychiatric diseases, but its effect on the liver, main site of drug metabolism,still  remain  unclear.  We  investigated  the  effects  of  chronic  treatment  of olanzapine   (three-week)   on   the   malondialdehyde   (MDA)   content   and protein expression and activity of antioxidant enzyme catalase (CAT) in the liver of rats exposed to chronic social isolation (CSIS) (six-week), an animal model of depression. The increased cytosolic MDA content in both vehicle-and  olanzapine-treated  CSIS  animals  suggests  oxidative  stress. Increased CAT  activityin  vehicle-treated  CSIS  animals,  which  was  not  consistent with   its   protein   expression,   suggests   induction   of   antioxidant   defense mechanisms,while  olanzapine  significantly  reduced  CAT  activity  in  CSIS group.   Data   revealed   that   although   olanzapine   treatment   reversed   the alterations  in  CAT  activity,  it  has the  ability  to  cause  hepatotoxicity,  as indicatedby increased MDA content.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry
T1  - Olanzapine intensifies lipid peroxidation and modulates catalase activity in liver of social isolated rats
SP  - 439
EP  - 442
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9203
ER  - 

@conference{
author = "Stanisavljević, Andrijana and Perić, Ivana and Todorović, Nevena and Đorđević, Neda O. and Filipović, Dragana",
year = "2016",
abstract = "Olanzapine  is  an  antipsychotic  proved  to  be  effective  in stress  associated psychiatric diseases, but its effect on the liver, main site of drug metabolism,still  remain  unclear.  We  investigated  the  effects  of  chronic  treatment  of olanzapine   (three-week)   on   the   malondialdehyde   (MDA)   content   and protein expression and activity of antioxidant enzyme catalase (CAT) in the liver of rats exposed to chronic social isolation (CSIS) (six-week), an animal model of depression. The increased cytosolic MDA content in both vehicle-and  olanzapine-treated  CSIS  animals  suggests  oxidative  stress. Increased CAT  activityin  vehicle-treated  CSIS  animals,  which  was  not  consistent with   its   protein   expression,   suggests   induction   of   antioxidant   defense mechanisms,while  olanzapine  significantly  reduced  CAT  activity  in  CSIS group.   Data   revealed   that   although   olanzapine   treatment   reversed   the alterations  in  CAT  activity,  it  has the  ability  to  cause  hepatotoxicity,  as indicatedby increased MDA content.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry",
title = "Olanzapine intensifies lipid peroxidation and modulates catalase activity in liver of social isolated rats",
pages = "439-442",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9203"
}

Stanisavljević, A., Perić, I., Todorović, N., Đorđević, N. O.,& Filipović, D.. (2016). Olanzapine intensifies lipid peroxidation and modulates catalase activity in liver of social isolated rats. in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 439-442.
https://hdl.handle.net/21.15107/rcub_vinar_9203

Stanisavljević A, Perić I, Todorović N, Đorđević NO, Filipović D. Olanzapine intensifies lipid peroxidation and modulates catalase activity in liver of social isolated rats. in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry. 2016;:439-442.
https://hdl.handle.net/21.15107/rcub_vinar_9203 .

Stanisavljević, Andrijana, Perić, Ivana, Todorović, Nevena, Đorđević, Neda O., Filipović, Dragana, "Olanzapine intensifies lipid peroxidation and modulates catalase activity in liver of social isolated rats" in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry (2016):439-442,
https://hdl.handle.net/21.15107/rcub_vinar_9203 .

TY  - CONF
AU  - Đorđević, Neda O.
AU  - Perić, Ivana
AU  - Stanisavljević, Andrijana
AU  - Todorović, Nevena
AU  - Filipović, Dragana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9205
AB  - Duloxetine  (DLX)  is  antidepressantfor  the  treatment  of  depression,but  its effect  on  the  liver,  a  primary  site  for  drug  metabolism,  has  yet  to  be determined.The  effect  of  3  weeks  of  DLX  treatment  onprotein  carbonyl groups   and   activities   of   GSH-dependent   defense   including   reduced glutathione   (GSH),   glutathione   peroxidase   (GPx)   and   glutathione   S-transferase  (GST)  in  liver  of  rats  exposed  to  6  weeks  of  chronic  social isolation  (CSIS),  an  animal  model  of  depression,  were  investigated.  CSIS induced increase in protein carbonyl content, which wasdecreasedby DLX treatment.  We  noticed  increase  in  GPx  and  GST  activity  in  DLX-treated (controls  and  CSIS)  rats  and  CSIS  group,  whereby  GPx  activity  was significantly higher in DLX-compared to vehicle-treatedCSIS rats. Results indicate protective effect of DLX against CSIS-induced oxidative damage of hepatic  proteins,  which  may  be  due  to  intensified  protective  mechanisms mediated by GSH-dependent defense.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry
T1  - Duloxetine enhances hepatic gsh-dependent defense in rats
SP  - 443
EP  - 446
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9205
ER  - 

@conference{
author = "Đorđević, Neda O. and Perić, Ivana and Stanisavljević, Andrijana and Todorović, Nevena and Filipović, Dragana",
year = "2016",
abstract = "Duloxetine  (DLX)  is  antidepressantfor  the  treatment  of  depression,but  its effect  on  the  liver,  a  primary  site  for  drug  metabolism,  has  yet  to  be determined.The  effect  of  3  weeks  of  DLX  treatment  onprotein  carbonyl groups   and   activities   of   GSH-dependent   defense   including   reduced glutathione   (GSH),   glutathione   peroxidase   (GPx)   and   glutathione   S-transferase  (GST)  in  liver  of  rats  exposed  to  6  weeks  of  chronic  social isolation  (CSIS),  an  animal  model  of  depression,  were  investigated.  CSIS induced increase in protein carbonyl content, which wasdecreasedby DLX treatment.  We  noticed  increase  in  GPx  and  GST  activity  in  DLX-treated (controls  and  CSIS)  rats  and  CSIS  group,  whereby  GPx  activity  was significantly higher in DLX-compared to vehicle-treatedCSIS rats. Results indicate protective effect of DLX against CSIS-induced oxidative damage of hepatic  proteins,  which  may  be  due  to  intensified  protective  mechanisms mediated by GSH-dependent defense.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry",
title = "Duloxetine enhances hepatic gsh-dependent defense in rats",
pages = "443-446",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9205"
}

Đorđević, N. O., Perić, I., Stanisavljević, A., Todorović, N.,& Filipović, D.. (2016). Duloxetine enhances hepatic gsh-dependent defense in rats. in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 443-446.
https://hdl.handle.net/21.15107/rcub_vinar_9205

Đorđević NO, Perić I, Stanisavljević A, Todorović N, Filipović D. Duloxetine enhances hepatic gsh-dependent defense in rats. in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry. 2016;:443-446.
https://hdl.handle.net/21.15107/rcub_vinar_9205 .

Đorđević, Neda O., Perić, Ivana, Stanisavljević, Andrijana, Todorović, Nevena, Filipović, Dragana, "Duloxetine enhances hepatic gsh-dependent defense in rats" in Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry (2016):443-446,
https://hdl.handle.net/21.15107/rcub_vinar_9205 .

TY  - JOUR
AU  - Todorović, Nevena
AU  - Tomanović, Nada
AU  - Gass, Peter
AU  - Filipović, Dragana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/883
AB  - Olanzapine, an atypical antipsychotic, is efficient in stress associated psychiatric diseases, but its effect on the liver, a primary organ for drug activation and detoxification, still remains unclear. The effect of olanzapine administration (7.5 mg/kg/day), on rat hepatic glutathione (GSH)-dependent defense and proinflammatory cytokines following 6 weeks of chronic social isolation (CSIS), which causes depressive-and anxiety-like behavior in adult male Wistar rats, was investigated. The subcellular distribution of nuclear factor-kappa B (NF-kappa B), cytosolic inducible nitric oxide synthase (iNOS) protein levels and hepatic histological alterations were also determined. Decreased GSH content and glutathione reductase activity associated with increased catalase and glutathione S-transferase activity following CSIS indicated hepatic oxidative stress. Moreover, CSIS caused NF-kappa B nuclear translocation and the concomitant increase in iNOS together with increase in interleukin-1beta and tumor necrosis factor alpha protein levels, but no effect on interleukin-6. Olanzapine treatment suppressed NF-kappa B activation and iNOS expression and caused modulation of GSH-dependent defense systems but failed to reverse CSIS-induced increase in hepatic proinflammatory cytokines. Portal inflammation, focal hepatocyte necrosis and an increased number of Kupffer cells in CSIS rats (vehicle-or olanzapine-treated) were found. Olanzapine-treated socially reared rats showed portal inflammation and focal hepatocyte necrosis. Data suggest that CSIS compromised GSH-dependent defense, triggered a proinflammatory response and histological alterations in rat liver. Olanzapine treatment partially reversed the alterations in hepatic GSH-dependent defense, but showed no anti-inflammatory effect suggesting that it may provide protective effect against hepatic CSIS-induced oxidative stress, but not against inflammation. (C) 2015 Elsevier B.V. All rights reserved.
PB  - Elsevier
T2  - European Journal of Pharmaceutical Sciences
T1  - Olanzapine modulation of hepatic oxidative stress and inflammation in socially isolated rats
VL  - 81
SP  - 94
EP  - 102
DO  - 10.1016/j.ejps.2015.10.010
ER  - 

@article{
author = "Todorović, Nevena and Tomanović, Nada and Gass, Peter and Filipović, Dragana",
year = "2016",
abstract = "Olanzapine, an atypical antipsychotic, is efficient in stress associated psychiatric diseases, but its effect on the liver, a primary organ for drug activation and detoxification, still remains unclear. The effect of olanzapine administration (7.5 mg/kg/day), on rat hepatic glutathione (GSH)-dependent defense and proinflammatory cytokines following 6 weeks of chronic social isolation (CSIS), which causes depressive-and anxiety-like behavior in adult male Wistar rats, was investigated. The subcellular distribution of nuclear factor-kappa B (NF-kappa B), cytosolic inducible nitric oxide synthase (iNOS) protein levels and hepatic histological alterations were also determined. Decreased GSH content and glutathione reductase activity associated with increased catalase and glutathione S-transferase activity following CSIS indicated hepatic oxidative stress. Moreover, CSIS caused NF-kappa B nuclear translocation and the concomitant increase in iNOS together with increase in interleukin-1beta and tumor necrosis factor alpha protein levels, but no effect on interleukin-6. Olanzapine treatment suppressed NF-kappa B activation and iNOS expression and caused modulation of GSH-dependent defense systems but failed to reverse CSIS-induced increase in hepatic proinflammatory cytokines. Portal inflammation, focal hepatocyte necrosis and an increased number of Kupffer cells in CSIS rats (vehicle-or olanzapine-treated) were found. Olanzapine-treated socially reared rats showed portal inflammation and focal hepatocyte necrosis. Data suggest that CSIS compromised GSH-dependent defense, triggered a proinflammatory response and histological alterations in rat liver. Olanzapine treatment partially reversed the alterations in hepatic GSH-dependent defense, but showed no anti-inflammatory effect suggesting that it may provide protective effect against hepatic CSIS-induced oxidative stress, but not against inflammation. (C) 2015 Elsevier B.V. All rights reserved.",
publisher = "Elsevier",
journal = "European Journal of Pharmaceutical Sciences",
title = "Olanzapine modulation of hepatic oxidative stress and inflammation in socially isolated rats",
volume = "81",
pages = "94-102",
doi = "10.1016/j.ejps.2015.10.010"
}

Todorović, N., Tomanović, N., Gass, P.,& Filipović, D.. (2016). Olanzapine modulation of hepatic oxidative stress and inflammation in socially isolated rats. in European Journal of Pharmaceutical Sciences
Elsevier., 81, 94-102.
https://doi.org/10.1016/j.ejps.2015.10.010

Todorović N, Tomanović N, Gass P, Filipović D. Olanzapine modulation of hepatic oxidative stress and inflammation in socially isolated rats. in European Journal of Pharmaceutical Sciences. 2016;81:94-102.
doi:10.1016/j.ejps.2015.10.010 .

Todorović, Nevena, Tomanović, Nada, Gass, Peter, Filipović, Dragana, "Olanzapine modulation of hepatic oxidative stress and inflammation in socially isolated rats" in European Journal of Pharmaceutical Sciences, 81 (2016):94-102,
https://doi.org/10.1016/j.ejps.2015.10.010 . .

TY  - JOUR
AU  - Petrusic, Vladimir
AU  - Todorović, Nevena
AU  - Zivkovic, Irena
AU  - Dimitrijevic, Rajna
AU  - Muhandes, Lina
AU  - Rajnpreht, Irena
AU  - Dimitrijevic, Ljiljana
PY  - 2015
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/465
AB  - Recent data concerning antiphospholipid syndrome (APS) induction have shown that beta(2)-glycoprotein I (beta(2)GPI) binds lipopolysaccharide (LPS), which results in conformational changes, exposition of a cryptic epitope and possible pathological anti-beta(2)GPI antibody production. In order to investigate the effects of LPS on the induction of APS-related pathology, we performed hyperimmunization of BALB/c and C57BL/6 mice with LPS, alone or in combination with tetanus toxoid (TTd), a protein structurally similar to beta(2)GPI. We report that, although high affinity pathological anti-beta(2)GPI antibodies were produced in all groups of animals, the reproductive pathology was recorded only in mice that received both LPS and TTd, implying on the important roles of both infections and molecular mimicry in APS pathogenesis. Moreover, APS-related reproductive pathology was more pronounced in BALB/c (lowered fertility and fecundity) than C57BL/6 mice (lowered fecundity), which correlated well with the disruption in natural antibody network observed in BALB/c mouse strain.
T2  - Autoimmunity
T1  - Autoantibody response and pregnancy-related pathology induced by combined LPS and tetanus toxoid hyperimmunization in BALB/c and C57BL/6 mice
VL  - 48
IS  - 2
SP  - 87
EP  - 99
DO  - 10.3109/08916934.2014.961061
ER  - 

@article{
author = "Petrusic, Vladimir and Todorović, Nevena and Zivkovic, Irena and Dimitrijevic, Rajna and Muhandes, Lina and Rajnpreht, Irena and Dimitrijevic, Ljiljana",
year = "2015",
abstract = "Recent data concerning antiphospholipid syndrome (APS) induction have shown that beta(2)-glycoprotein I (beta(2)GPI) binds lipopolysaccharide (LPS), which results in conformational changes, exposition of a cryptic epitope and possible pathological anti-beta(2)GPI antibody production. In order to investigate the effects of LPS on the induction of APS-related pathology, we performed hyperimmunization of BALB/c and C57BL/6 mice with LPS, alone or in combination with tetanus toxoid (TTd), a protein structurally similar to beta(2)GPI. We report that, although high affinity pathological anti-beta(2)GPI antibodies were produced in all groups of animals, the reproductive pathology was recorded only in mice that received both LPS and TTd, implying on the important roles of both infections and molecular mimicry in APS pathogenesis. Moreover, APS-related reproductive pathology was more pronounced in BALB/c (lowered fertility and fecundity) than C57BL/6 mice (lowered fecundity), which correlated well with the disruption in natural antibody network observed in BALB/c mouse strain.",
journal = "Autoimmunity",
title = "Autoantibody response and pregnancy-related pathology induced by combined LPS and tetanus toxoid hyperimmunization in BALB/c and C57BL/6 mice",
volume = "48",
number = "2",
pages = "87-99",
doi = "10.3109/08916934.2014.961061"
}

Petrusic, V., Todorović, N., Zivkovic, I., Dimitrijevic, R., Muhandes, L., Rajnpreht, I.,& Dimitrijevic, L.. (2015). Autoantibody response and pregnancy-related pathology induced by combined LPS and tetanus toxoid hyperimmunization in BALB/c and C57BL/6 mice. in Autoimmunity, 48(2), 87-99.
https://doi.org/10.3109/08916934.2014.961061

Petrusic V, Todorović N, Zivkovic I, Dimitrijevic R, Muhandes L, Rajnpreht I, Dimitrijevic L. Autoantibody response and pregnancy-related pathology induced by combined LPS and tetanus toxoid hyperimmunization in BALB/c and C57BL/6 mice. in Autoimmunity. 2015;48(2):87-99.
doi:10.3109/08916934.2014.961061 .

Petrusic, Vladimir, Todorović, Nevena, Zivkovic, Irena, Dimitrijevic, Rajna, Muhandes, Lina, Rajnpreht, Irena, Dimitrijevic, Ljiljana, "Autoantibody response and pregnancy-related pathology induced by combined LPS and tetanus toxoid hyperimmunization in BALB/c and C57BL/6 mice" in Autoimmunity, 48, no. 2 (2015):87-99,
https://doi.org/10.3109/08916934.2014.961061 . .

TY  - JOUR
AU  - Martinović, Jelena
AU  - Todorović, Nevena
AU  - Bošković, Maja
AU  - Pajović, Snežana B.
AU  - Demajo, Miroslav
AU  - Filipović, Dragana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6058
AB  - Chronic oxidative stress plays an important role in depression. The aim of present study was to examine the stress-induced changes in serum corticosterone (CORT) levels, cytosolic protein carbonyl groups, malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide (NO) and total superoxide dismutase (SOD) activity in the prefrontal cortex versus hippocampus of male Wistar rats exposed to acute (2 h of immobilization or cold), chronic (21d of social isolation) stress, and their combination (chronic + acute stress). The subcellular distribution of nuclear factor-kappa B (NF-kappa B) and cytosolic cyclooxygenase 2 (COX-2) protein expressions were also examined. Depressive- and anxiety-like behaviors were assessed via the forced swim, sucrose preference, and marble burying tests in chronically isolated rats. Although both acute stressors resulted in elevated CORT, increased MDA in the prefrontal cortex and NF-kappa B activation accompanied by increased NO in the hippocampus were detected only following acute cold stress. Chronic isolation resulted in no change in CORT levels, but disabled appropriate response to novel acute stress and led to depressive- and anxiety-like behaviors. Increased oxidative/nitrosative stress markers, likely by NF-kappa B nuclear translocation and concomitant COX-2 upregulation, associated with decreased SOD activity and GSH levels, suggested the existence of oxidative stress in the prefrontal cortex. In contrast, hippocampus was less susceptible to oxidative damage showing only increase in protein carbonyl groups and depleted GSH. Taken together, the prefrontal cortex seems to be more sensitive to oxidative stress than the hippocampus following chronic isolation stress, which may be relevant for further research related to stress-induced depressive-like behavior.
T2  - Molecular and Cellular Biochemistry
T1  - Different susceptibility of prefrontal cortex and hippocampus to oxidative stress following chronic social isolation stress
VL  - 393
IS  - 1-2
SP  - 43
EP  - 57
DO  - 10.1007/s11010-014-2045-z
ER  - 

@article{
author = "Martinović, Jelena and Todorović, Nevena and Bošković, Maja and Pajović, Snežana B. and Demajo, Miroslav and Filipović, Dragana",
year = "2014",
abstract = "Chronic oxidative stress plays an important role in depression. The aim of present study was to examine the stress-induced changes in serum corticosterone (CORT) levels, cytosolic protein carbonyl groups, malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide (NO) and total superoxide dismutase (SOD) activity in the prefrontal cortex versus hippocampus of male Wistar rats exposed to acute (2 h of immobilization or cold), chronic (21d of social isolation) stress, and their combination (chronic + acute stress). The subcellular distribution of nuclear factor-kappa B (NF-kappa B) and cytosolic cyclooxygenase 2 (COX-2) protein expressions were also examined. Depressive- and anxiety-like behaviors were assessed via the forced swim, sucrose preference, and marble burying tests in chronically isolated rats. Although both acute stressors resulted in elevated CORT, increased MDA in the prefrontal cortex and NF-kappa B activation accompanied by increased NO in the hippocampus were detected only following acute cold stress. Chronic isolation resulted in no change in CORT levels, but disabled appropriate response to novel acute stress and led to depressive- and anxiety-like behaviors. Increased oxidative/nitrosative stress markers, likely by NF-kappa B nuclear translocation and concomitant COX-2 upregulation, associated with decreased SOD activity and GSH levels, suggested the existence of oxidative stress in the prefrontal cortex. In contrast, hippocampus was less susceptible to oxidative damage showing only increase in protein carbonyl groups and depleted GSH. Taken together, the prefrontal cortex seems to be more sensitive to oxidative stress than the hippocampus following chronic isolation stress, which may be relevant for further research related to stress-induced depressive-like behavior.",
journal = "Molecular and Cellular Biochemistry",
title = "Different susceptibility of prefrontal cortex and hippocampus to oxidative stress following chronic social isolation stress",
volume = "393",
number = "1-2",
pages = "43-57",
doi = "10.1007/s11010-014-2045-z"
}

Martinović, J., Todorović, N., Bošković, M., Pajović, S. B., Demajo, M.,& Filipović, D.. (2014). Different susceptibility of prefrontal cortex and hippocampus to oxidative stress following chronic social isolation stress. in Molecular and Cellular Biochemistry, 393(1-2), 43-57.
https://doi.org/10.1007/s11010-014-2045-z

Martinović J, Todorović N, Bošković M, Pajović SB, Demajo M, Filipović D. Different susceptibility of prefrontal cortex and hippocampus to oxidative stress following chronic social isolation stress. in Molecular and Cellular Biochemistry. 2014;393(1-2):43-57.
doi:10.1007/s11010-014-2045-z .

Martinović, Jelena, Todorović, Nevena, Bošković, Maja, Pajović, Snežana B., Demajo, Miroslav, Filipović, Dragana, "Different susceptibility of prefrontal cortex and hippocampus to oxidative stress following chronic social isolation stress" in Molecular and Cellular Biochemistry, 393, no. 1-2 (2014):43-57,
https://doi.org/10.1007/s11010-014-2045-z . .

TY  - CONF
AU  - Todorović, Nevena
AU  - Stevanović, Jelena
AU  - Filipović, Dragana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9267
AB  - Chronic psychosocial stress is a risk factor for depression. As the liver is a
primary organ for drug activation and detoxification, the effect of chronic
administration of olanzapine on rat hepatic glutathione (GSH)-dependent
defense system including GSH, glutathione peroxidase (GPx) and
glutathione reductase (GLR), following 21d of chronic social isolation stress
(CSIS), an animal model of depression, was investigated. Increased GSH
content in olanzapine-treated controls was found. Protein levels of GPx
were increased in both vehicle- and olanzapine-treated chronically-isolated
animals while significant increase in GPx activity was seen only in
olanzapine-treated chronically-isolated animals. Reduced activity of GLR in
CSIS group that was not in correspondence with its protein level was
increased by olanzapine. The results imply that chronic administration of
olanzapine reverts the alternations in hepatic GSH-dependent defense of
stressed rats caused by CSIS.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - Olanzapine reverts the isolation-induced alterations in rat hepatic glutathione-dependent defense system
VL  - Q-05-P
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9267
ER  - 

@conference{
author = "Todorović, Nevena and Stevanović, Jelena and Filipović, Dragana",
year = "2014",
abstract = "Chronic psychosocial stress is a risk factor for depression. As the liver is a
primary organ for drug activation and detoxification, the effect of chronic
administration of olanzapine on rat hepatic glutathione (GSH)-dependent
defense system including GSH, glutathione peroxidase (GPx) and
glutathione reductase (GLR), following 21d of chronic social isolation stress
(CSIS), an animal model of depression, was investigated. Increased GSH
content in olanzapine-treated controls was found. Protein levels of GPx
were increased in both vehicle- and olanzapine-treated chronically-isolated
animals while significant increase in GPx activity was seen only in
olanzapine-treated chronically-isolated animals. Reduced activity of GLR in
CSIS group that was not in correspondence with its protein level was
increased by olanzapine. The results imply that chronic administration of
olanzapine reverts the alternations in hepatic GSH-dependent defense of
stressed rats caused by CSIS.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "Olanzapine reverts the isolation-induced alterations in rat hepatic glutathione-dependent defense system",
volume = "Q-05-P",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9267"
}

Todorović, N., Stevanović, J.,& Filipović, D.. (2014). Olanzapine reverts the isolation-induced alterations in rat hepatic glutathione-dependent defense system. in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia., Q-05-P.
https://hdl.handle.net/21.15107/rcub_vinar_9267

Todorović N, Stevanović J, Filipović D. Olanzapine reverts the isolation-induced alterations in rat hepatic glutathione-dependent defense system. in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2014;Q-05-P.
https://hdl.handle.net/21.15107/rcub_vinar_9267 .

Todorović, Nevena, Stevanović, Jelena, Filipović, Dragana, "Olanzapine reverts the isolation-induced alterations in rat hepatic glutathione-dependent defense system" in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry, Q-05-P (2014),
https://hdl.handle.net/21.15107/rcub_vinar_9267 .

TY  - CONF
AU  - Bošković, Maja
AU  - Todorović, Nevena
AU  - Filipović, Dragana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9266
AB  - Changes in glutathione (GSH)-related systems are implicated in depressive
disorders. Since chronic psychosocial stress contributes to depression, we
investigated the effects of 21d of chronic social isolation (CSIS) stress, an
animal model of depression, as well as chronic administration of clozapine,
an atypical antipsychotic, on GSH content, glutathione peroxidase (GPx)
and glutathione reductase (GLR) in the prefrontal cortex of rats. Increased
GPx protein expression and its activity in clozapine-treated (controls or
chronically-isolated) rats as well as in CSIS group were found. Nonetheless,
clozapine administration caused decrease in GSH content but no effects on
GLR in controls and CSIS group. Data indicate that CSIS compromises
GSH-dependent redox system promoting oxidative stress in rat prefrontal
cortex which can’t be protected by clozapine. Moreover, clozapine
administration in controls has a harmful side effect on this redox system.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - Compromised glutathione-dependent redox system of chronically-isolated rats: a harmful side effect of clozapine
SP  - 1113
EP  - 1116
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9266
ER  - 

@conference{
author = "Bošković, Maja and Todorović, Nevena and Filipović, Dragana",
year = "2014",
abstract = "Changes in glutathione (GSH)-related systems are implicated in depressive
disorders. Since chronic psychosocial stress contributes to depression, we
investigated the effects of 21d of chronic social isolation (CSIS) stress, an
animal model of depression, as well as chronic administration of clozapine,
an atypical antipsychotic, on GSH content, glutathione peroxidase (GPx)
and glutathione reductase (GLR) in the prefrontal cortex of rats. Increased
GPx protein expression and its activity in clozapine-treated (controls or
chronically-isolated) rats as well as in CSIS group were found. Nonetheless,
clozapine administration caused decrease in GSH content but no effects on
GLR in controls and CSIS group. Data indicate that CSIS compromises
GSH-dependent redox system promoting oxidative stress in rat prefrontal
cortex which can’t be protected by clozapine. Moreover, clozapine
administration in controls has a harmful side effect on this redox system.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "Compromised glutathione-dependent redox system of chronically-isolated rats: a harmful side effect of clozapine",
pages = "1113-1116",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9266"
}

Bošković, M., Todorović, N.,& Filipović, D.. (2014). Compromised glutathione-dependent redox system of chronically-isolated rats: a harmful side effect of clozapine. in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia., 1113-1116.
https://hdl.handle.net/21.15107/rcub_vinar_9266

Bošković M, Todorović N, Filipović D. Compromised glutathione-dependent redox system of chronically-isolated rats: a harmful side effect of clozapine. in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2014;:1113-1116.
https://hdl.handle.net/21.15107/rcub_vinar_9266 .

Bošković, Maja, Todorović, Nevena, Filipović, Dragana, "Compromised glutathione-dependent redox system of chronically-isolated rats: a harmful side effect of clozapine" in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry (2014):1113-1116,
https://hdl.handle.net/21.15107/rcub_vinar_9266 .

TY  - CONF
AU  - Todorović, Nevena
AU  - Bošković, Maja
AU  - Filipović, Dragana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9269
AB  - Chronic exposure to psychosocial stress is implicated in the
pathophysiology of depression. We investigated the effect of 21d of chronic
social isolation (CSIS) stress (an animal model of depression) and/or
chronic administration of fluoxetine (15 mg/kg/day), an antidepressant, on
GSH content, protein expression and activity of glutathione peroxidase
(GPx) and glutathione reductase (GLR) in the cytosolic fraction of rat
hippocampus. CSIS stress caused reduced GPx and GLR protein expression
which was not prevented with fluoxetine treatment. Moreover, fluoxetine
administration intensified reduction of these proteins expression. Decreased
GSH content, GPx and GLR activity was also found in chronically-isolated
animals (vehicle- or fluoxetine treated). Data indicate that fluoxetine not
only failed to prevent CSIS-induced changes but itself compromised GSHdependent
defense system in control animals.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - Fluoxetine failed to prevent isolation-induced changes of glutathione-dependent defense in rat hippocampus
VL  - Q-06-P
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9269
ER  - 

@conference{
author = "Todorović, Nevena and Bošković, Maja and Filipović, Dragana",
year = "2014",
abstract = "Chronic exposure to psychosocial stress is implicated in the
pathophysiology of depression. We investigated the effect of 21d of chronic
social isolation (CSIS) stress (an animal model of depression) and/or
chronic administration of fluoxetine (15 mg/kg/day), an antidepressant, on
GSH content, protein expression and activity of glutathione peroxidase
(GPx) and glutathione reductase (GLR) in the cytosolic fraction of rat
hippocampus. CSIS stress caused reduced GPx and GLR protein expression
which was not prevented with fluoxetine treatment. Moreover, fluoxetine
administration intensified reduction of these proteins expression. Decreased
GSH content, GPx and GLR activity was also found in chronically-isolated
animals (vehicle- or fluoxetine treated). Data indicate that fluoxetine not
only failed to prevent CSIS-induced changes but itself compromised GSHdependent
defense system in control animals.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "Fluoxetine failed to prevent isolation-induced changes of glutathione-dependent defense in rat hippocampus",
volume = "Q-06-P",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9269"
}

Todorović, N., Bošković, M.,& Filipović, D.. (2014). Fluoxetine failed to prevent isolation-induced changes of glutathione-dependent defense in rat hippocampus. in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia., Q-06-P.
https://hdl.handle.net/21.15107/rcub_vinar_9269

Todorović N, Bošković M, Filipović D. Fluoxetine failed to prevent isolation-induced changes of glutathione-dependent defense in rat hippocampus. in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2014;Q-06-P.
https://hdl.handle.net/21.15107/rcub_vinar_9269 .

Todorović, Nevena, Bošković, Maja, Filipović, Dragana, "Fluoxetine failed to prevent isolation-induced changes of glutathione-dependent defense in rat hippocampus" in Physical chemistry 2014: 12th International Conference on Fundamental and Applied Aspects of Physical Chemistry, Q-06-P (2014),
https://hdl.handle.net/21.15107/rcub_vinar_9269 .

TY  - JOUR
AU  - Martinović, Jelena
AU  - Todorović, Nevena
AU  - Tomanović, Nada
AU  - Bošković, Maja
AU  - Djordjevic, Snezana
AU  - Lazarević-Pašti, Tamara
AU  - Bernardi, Rick E.
AU  - Đurđević, Aleksandra
AU  - Filipović, Dragana
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6043
AB  - Chronic exposure to stress contributes to the etiology of mood disorders, and the liver as a target organ of antidepressant and antipsychotic drug metabolism is vulnerable to drug-induced toxicity. We investigated the effects of chronic administration of fluoxetine (15 mg/kg/day) or clozapine (20 mg/kg/day) on liver injury via the measurement of liver enzymes, oxidative stress and histopathology in rats exposed to chronic social isolation (21 days), an animal model of depression, and controls. The activity of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), the liver content of carbonyl groups, malonyldialdehyde (MDA), reduced glutathione (GSH), cytosolic glutathione S-transferase (GST) and nitric oxide (NO) metabolites were determined. We also characterized nuclear factor-kappa B (NF-kappa B), cyclooxygenase-2 (COX-2) and CuZn-superoxide dismutase (CuZnSOD) protein expression as well as histopathological changes. Increased serum ALT activity in chronically-isolated and control animals treated with both drugs was found while increased AST activity was observed only in fluoxetine-treated rats (chronically-isolated and controls). Increased carbonyl content, MDA, GST activity and decreased GSH levels in drug-treated controls/chronically-isolated animals suggest a link between drugs and hepatic oxidative stress. Increased NO levels associated with NF-kappa B activation and the concomitant increased COX-2 expression together with compromised CuZnSOD expression in clozapine-treated chronically-isolated rats likely reinforce oxidative stress, observed by increased lipid peroxidation and GSH depletion. In contrast, fluoxetine reduced NO levels in chronically-isolated rats. Isolation induced oxidative stress but histological changes were similar to those observed in vehicle-treated controls. Chronic administration of fluoxetine in both chronically-isolated and control animals resulted in more or less normal hepatic architecture, while clozapine in both groups resulted in liver injury. These data suggest that clozapine appears to have a higher potential to induce liver toxicity than fluoxetine. (C) 2014 Elsevier B.V. All rights reserved.
T2  - European Journal of Pharmaceutical Sciences
T1  - Chronic administration of fluoxetine or clozapine induces oxidative stress in rat liver: A histopathological study
VL  - 59
SP  - 20
EP  - 30
DO  - 10.1016/j.ejps.2014.04.010
ER  - 

@article{
author = "Martinović, Jelena and Todorović, Nevena and Tomanović, Nada and Bošković, Maja and Djordjevic, Snezana and Lazarević-Pašti, Tamara and Bernardi, Rick E. and Đurđević, Aleksandra and Filipović, Dragana",
year = "2014",
abstract = "Chronic exposure to stress contributes to the etiology of mood disorders, and the liver as a target organ of antidepressant and antipsychotic drug metabolism is vulnerable to drug-induced toxicity. We investigated the effects of chronic administration of fluoxetine (15 mg/kg/day) or clozapine (20 mg/kg/day) on liver injury via the measurement of liver enzymes, oxidative stress and histopathology in rats exposed to chronic social isolation (21 days), an animal model of depression, and controls. The activity of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), the liver content of carbonyl groups, malonyldialdehyde (MDA), reduced glutathione (GSH), cytosolic glutathione S-transferase (GST) and nitric oxide (NO) metabolites were determined. We also characterized nuclear factor-kappa B (NF-kappa B), cyclooxygenase-2 (COX-2) and CuZn-superoxide dismutase (CuZnSOD) protein expression as well as histopathological changes. Increased serum ALT activity in chronically-isolated and control animals treated with both drugs was found while increased AST activity was observed only in fluoxetine-treated rats (chronically-isolated and controls). Increased carbonyl content, MDA, GST activity and decreased GSH levels in drug-treated controls/chronically-isolated animals suggest a link between drugs and hepatic oxidative stress. Increased NO levels associated with NF-kappa B activation and the concomitant increased COX-2 expression together with compromised CuZnSOD expression in clozapine-treated chronically-isolated rats likely reinforce oxidative stress, observed by increased lipid peroxidation and GSH depletion. In contrast, fluoxetine reduced NO levels in chronically-isolated rats. Isolation induced oxidative stress but histological changes were similar to those observed in vehicle-treated controls. Chronic administration of fluoxetine in both chronically-isolated and control animals resulted in more or less normal hepatic architecture, while clozapine in both groups resulted in liver injury. These data suggest that clozapine appears to have a higher potential to induce liver toxicity than fluoxetine. (C) 2014 Elsevier B.V. All rights reserved.",
journal = "European Journal of Pharmaceutical Sciences",
title = "Chronic administration of fluoxetine or clozapine induces oxidative stress in rat liver: A histopathological study",
volume = "59",
pages = "20-30",
doi = "10.1016/j.ejps.2014.04.010"
}

Martinović, J., Todorović, N., Tomanović, N., Bošković, M., Djordjevic, S., Lazarević-Pašti, T., Bernardi, R. E., Đurđević, A.,& Filipović, D.. (2014). Chronic administration of fluoxetine or clozapine induces oxidative stress in rat liver: A histopathological study. in European Journal of Pharmaceutical Sciences, 59, 20-30.
https://doi.org/10.1016/j.ejps.2014.04.010

Martinović J, Todorović N, Tomanović N, Bošković M, Djordjevic S, Lazarević-Pašti T, Bernardi RE, Đurđević A, Filipović D. Chronic administration of fluoxetine or clozapine induces oxidative stress in rat liver: A histopathological study. in European Journal of Pharmaceutical Sciences. 2014;59:20-30.
doi:10.1016/j.ejps.2014.04.010 .

Martinović, Jelena, Todorović, Nevena, Tomanović, Nada, Bošković, Maja, Djordjevic, Snezana, Lazarević-Pašti, Tamara, Bernardi, Rick E., Đurđević, Aleksandra, Filipović, Dragana, "Chronic administration of fluoxetine or clozapine induces oxidative stress in rat liver: A histopathological study" in European Journal of Pharmaceutical Sciences, 59 (2014):20-30,
https://doi.org/10.1016/j.ejps.2014.04.010 . .