Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm
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2021
Authors
Marinković, JelenaNikolić, Biljana
Marković, Tatjana
Radunović, Milena
Ilić, Jugoslav
Bošković, Marko
Ćirić, Ana
Marković, Dejan
Article (Published version)
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Aim: The objective was to formulate and characterize the nanoemulsion based on Cymbopogon citratus oil, intended for use in infected teeth root canal therapy. The investigation of the antioxidant and antibiofilm potential toward Enterococcus faecalis was aimed as well. Materials & methods: Characterization of oil (by GC/MS analysis) and nanoemulsion (by dynamic light scattering instrument), and determination of antibacterial (by microdilution assay), antibiofilm (by crystal violet assay) and antioxidant properties (by 2,2-diphenyl-1-picryl-hydrazyl-hydrate and thiobarbituric acid assay methods) were provided. Antibiofilm efficacy of irrigation procedure including nanoemulsion was screened on extracted teeth (by CFU-counting assay). Results: Notable antibacterial and antibiofilm activity, both against forming and preformed biofilms of oil, was observed. Irrigation involved nanoemulsion showed remarkable antibiofilm potential. Both substances induced some antioxidant activity. Conclusion...: Results encourage further research with the aim of application of the nanoemulsion in dental practice.
Keywords:
antibiofilm activity / Cymbopogon citratus essential oil / Enterococcus faecalis / infected teeth root canal / nanoemulsionSource:
Future Microbiology, 2021, 16, 12, 907-918Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200017 (University of Belgrade, Institute of Nuclear Sciences 'Vinča', Belgrade-Vinča) (RS-MESTD-inst-2020-200017)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200178 (University of Belgrade, Faculty of Biology) (RS-MESTD-inst-2020-200178)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200003 (Institute for Medicinal Plant Research 'Dr. Josif Pančić ', Belgrade) (RS-MESTD-inst-2020-200003)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
DOI: 10.2217/fmb-2021-0081
ISSN: 1746-0913
PubMed: 34319146
WoS: 000678169600001
Scopus: 2-s2.0-85112246620
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VinčaTY - JOUR AU - Marinković, Jelena AU - Nikolić, Biljana AU - Marković, Tatjana AU - Radunović, Milena AU - Ilić, Jugoslav AU - Bošković, Marko AU - Ćirić, Ana AU - Marković, Dejan PY - 2021 UR - https://vinar.vin.bg.ac.rs/handle/123456789/9906 AB - Aim: The objective was to formulate and characterize the nanoemulsion based on Cymbopogon citratus oil, intended for use in infected teeth root canal therapy. The investigation of the antioxidant and antibiofilm potential toward Enterococcus faecalis was aimed as well. Materials & methods: Characterization of oil (by GC/MS analysis) and nanoemulsion (by dynamic light scattering instrument), and determination of antibacterial (by microdilution assay), antibiofilm (by crystal violet assay) and antioxidant properties (by 2,2-diphenyl-1-picryl-hydrazyl-hydrate and thiobarbituric acid assay methods) were provided. Antibiofilm efficacy of irrigation procedure including nanoemulsion was screened on extracted teeth (by CFU-counting assay). Results: Notable antibacterial and antibiofilm activity, both against forming and preformed biofilms of oil, was observed. Irrigation involved nanoemulsion showed remarkable antibiofilm potential. Both substances induced some antioxidant activity. Conclusion: Results encourage further research with the aim of application of the nanoemulsion in dental practice. T2 - Future Microbiology T1 - Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm VL - 16 IS - 12 SP - 907 EP - 918 DO - 10.2217/fmb-2021-0081 ER -
@article{ author = "Marinković, Jelena and Nikolić, Biljana and Marković, Tatjana and Radunović, Milena and Ilić, Jugoslav and Bošković, Marko and Ćirić, Ana and Marković, Dejan", year = "2021", abstract = "Aim: The objective was to formulate and characterize the nanoemulsion based on Cymbopogon citratus oil, intended for use in infected teeth root canal therapy. The investigation of the antioxidant and antibiofilm potential toward Enterococcus faecalis was aimed as well. Materials & methods: Characterization of oil (by GC/MS analysis) and nanoemulsion (by dynamic light scattering instrument), and determination of antibacterial (by microdilution assay), antibiofilm (by crystal violet assay) and antioxidant properties (by 2,2-diphenyl-1-picryl-hydrazyl-hydrate and thiobarbituric acid assay methods) were provided. Antibiofilm efficacy of irrigation procedure including nanoemulsion was screened on extracted teeth (by CFU-counting assay). Results: Notable antibacterial and antibiofilm activity, both against forming and preformed biofilms of oil, was observed. Irrigation involved nanoemulsion showed remarkable antibiofilm potential. Both substances induced some antioxidant activity. Conclusion: Results encourage further research with the aim of application of the nanoemulsion in dental practice.", journal = "Future Microbiology", title = "Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm", volume = "16", number = "12", pages = "907-918", doi = "10.2217/fmb-2021-0081" }
Marinković, J., Nikolić, B., Marković, T., Radunović, M., Ilić, J., Bošković, M., Ćirić, A.,& Marković, D.. (2021). Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm. in Future Microbiology, 16(12), 907-918. https://doi.org/10.2217/fmb-2021-0081
Marinković J, Nikolić B, Marković T, Radunović M, Ilić J, Bošković M, Ćirić A, Marković D. Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm. in Future Microbiology. 2021;16(12):907-918. doi:10.2217/fmb-2021-0081 .
Marinković, Jelena, Nikolić, Biljana, Marković, Tatjana, Radunović, Milena, Ilić, Jugoslav, Bošković, Marko, Ćirić, Ana, Marković, Dejan, "Cymbopogon citratus essential oil: an active principle of nanoemulsion against Enterococcus faecalis root canal biofilm" in Future Microbiology, 16, no. 12 (2021):907-918, https://doi.org/10.2217/fmb-2021-0081 . .