Facile synthesis of L-cysteine functionalized graphene quantum dots as a bioimaging and photosensitive agent
2021
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Аутори
Milenković, MilaMišović, Aleksandra
Jovanović, Dragana J.
Popović-Bijelić, Ana D.
Ciasca, Gabriele
Romanò, Sabrina
Bonasera, Aurelio
Mojsin, Marija
Pejić, Jelena
Stevanović, Milena J.
Jovanović, Svetlana P.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Nowadays, a larger number of aggressive and corrosive chemical reagents as well as toxic solvents are used to achieve structural modification and cleaning of the final products. These lead to the production of residual, waste chemicals, which are often reactive, cancerogenic, and toxic to the environment. This study shows a new approach to the modification of graphene quantum dots (GQDs) using gamma irradiation where the usage of reagents was avoided. We achieved the incorporation of S and N atoms in the GQD structure by selecting an aqueous solution of L-cysteine as an irradiation medium. GQDs were exposed to gamma-irradiation at doses of 25, 50 and 200 kGy. After irradiation, the optical, structural, and morphological properties, as well as the possibility of their use as an agent in bioimaging and photodynamic therapy, were studied. We measured an enhanced quantum yield of photoluminescence with the highest dose of 25 kGy (21.60%). Both S- and N-functional groups were detected in al...l gamma-irradiated GQDs: amino, amide, thiol, and thione. Spin trap electron paramagnetic resonance showed that GQDs irradiated with 25 kGy can generate singlet oxygen upon illumination. Bioimaging on HeLa cells showed the best visibility for cells treated with GQDs irradiated with 25 kGy, while cytotoxicity was not detected after treatment of HeLa cells with gamma-irradiated GQDs.
Кључне речи:
atomic force microscopy / bioimaging / gamma irradiation / graphene quantum dots / photodynamic therapy / photoluminescenceИзвор:
Nanomaterials, 2021, 11, 8, 1879-Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200017 (Универзитет у Београду, Институт за нуклеарне науке Винча, Београд-Винча) (RS-MESTD-inst-2020-200017)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200042 (Универзитет у Београду, Институт за молекуларну генетику и генетичко инжењерство) (RS-MESTD-inst-2020-200042)
- Биомаркери у неуродегенеративним и малигним процесима (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41005)
- Italian Ministry of University and Research [CUP B78D19000280001]
DOI: 10.3390/nano11081879
ISSN: 2079-4991
PubMed: 34443709
WoS: 000690115800001
Scopus: 2-s2.0-85110612605
Колекције
Институција/група
VinčaTY - JOUR AU - Milenković, Mila AU - Mišović, Aleksandra AU - Jovanović, Dragana J. AU - Popović-Bijelić, Ana D. AU - Ciasca, Gabriele AU - Romanò, Sabrina AU - Bonasera, Aurelio AU - Mojsin, Marija AU - Pejić, Jelena AU - Stevanović, Milena J. AU - Jovanović, Svetlana P. PY - 2021 UR - https://vinar.vin.bg.ac.rs/handle/123456789/9873 AB - Nowadays, a larger number of aggressive and corrosive chemical reagents as well as toxic solvents are used to achieve structural modification and cleaning of the final products. These lead to the production of residual, waste chemicals, which are often reactive, cancerogenic, and toxic to the environment. This study shows a new approach to the modification of graphene quantum dots (GQDs) using gamma irradiation where the usage of reagents was avoided. We achieved the incorporation of S and N atoms in the GQD structure by selecting an aqueous solution of L-cysteine as an irradiation medium. GQDs were exposed to gamma-irradiation at doses of 25, 50 and 200 kGy. After irradiation, the optical, structural, and morphological properties, as well as the possibility of their use as an agent in bioimaging and photodynamic therapy, were studied. We measured an enhanced quantum yield of photoluminescence with the highest dose of 25 kGy (21.60%). Both S- and N-functional groups were detected in all gamma-irradiated GQDs: amino, amide, thiol, and thione. Spin trap electron paramagnetic resonance showed that GQDs irradiated with 25 kGy can generate singlet oxygen upon illumination. Bioimaging on HeLa cells showed the best visibility for cells treated with GQDs irradiated with 25 kGy, while cytotoxicity was not detected after treatment of HeLa cells with gamma-irradiated GQDs. T2 - Nanomaterials T1 - Facile synthesis of L-cysteine functionalized graphene quantum dots as a bioimaging and photosensitive agent VL - 11 IS - 8 SP - 1879 DO - 10.3390/nano11081879 ER -
@article{ author = "Milenković, Mila and Mišović, Aleksandra and Jovanović, Dragana J. and Popović-Bijelić, Ana D. and Ciasca, Gabriele and Romanò, Sabrina and Bonasera, Aurelio and Mojsin, Marija and Pejić, Jelena and Stevanović, Milena J. and Jovanović, Svetlana P.", year = "2021", abstract = "Nowadays, a larger number of aggressive and corrosive chemical reagents as well as toxic solvents are used to achieve structural modification and cleaning of the final products. These lead to the production of residual, waste chemicals, which are often reactive, cancerogenic, and toxic to the environment. This study shows a new approach to the modification of graphene quantum dots (GQDs) using gamma irradiation where the usage of reagents was avoided. We achieved the incorporation of S and N atoms in the GQD structure by selecting an aqueous solution of L-cysteine as an irradiation medium. GQDs were exposed to gamma-irradiation at doses of 25, 50 and 200 kGy. After irradiation, the optical, structural, and morphological properties, as well as the possibility of their use as an agent in bioimaging and photodynamic therapy, were studied. We measured an enhanced quantum yield of photoluminescence with the highest dose of 25 kGy (21.60%). Both S- and N-functional groups were detected in all gamma-irradiated GQDs: amino, amide, thiol, and thione. Spin trap electron paramagnetic resonance showed that GQDs irradiated with 25 kGy can generate singlet oxygen upon illumination. Bioimaging on HeLa cells showed the best visibility for cells treated with GQDs irradiated with 25 kGy, while cytotoxicity was not detected after treatment of HeLa cells with gamma-irradiated GQDs.", journal = "Nanomaterials", title = "Facile synthesis of L-cysteine functionalized graphene quantum dots as a bioimaging and photosensitive agent", volume = "11", number = "8", pages = "1879", doi = "10.3390/nano11081879" }
Milenković, M., Mišović, A., Jovanović, D. J., Popović-Bijelić, A. D., Ciasca, G., Romanò, S., Bonasera, A., Mojsin, M., Pejić, J., Stevanović, M. J.,& Jovanović, S. P.. (2021). Facile synthesis of L-cysteine functionalized graphene quantum dots as a bioimaging and photosensitive agent. in Nanomaterials, 11(8), 1879. https://doi.org/10.3390/nano11081879
Milenković M, Mišović A, Jovanović DJ, Popović-Bijelić AD, Ciasca G, Romanò S, Bonasera A, Mojsin M, Pejić J, Stevanović MJ, Jovanović SP. Facile synthesis of L-cysteine functionalized graphene quantum dots as a bioimaging and photosensitive agent. in Nanomaterials. 2021;11(8):1879. doi:10.3390/nano11081879 .
Milenković, Mila, Mišović, Aleksandra, Jovanović, Dragana J., Popović-Bijelić, Ana D., Ciasca, Gabriele, Romanò, Sabrina, Bonasera, Aurelio, Mojsin, Marija, Pejić, Jelena, Stevanović, Milena J., Jovanović, Svetlana P., "Facile synthesis of L-cysteine functionalized graphene quantum dots as a bioimaging and photosensitive agent" in Nanomaterials, 11, no. 8 (2021):1879, https://doi.org/10.3390/nano11081879 . .