Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles
Само за регистроване кориснике
2020
Аутори
Laban, Bojana B.Ralević, Uroš
Petrović, Sandra
Leskovac, Andreja
Vasić Anićijević, Dragana D.
Marković, Mirjana
Vasić, Vesna M.
Чланак у часопису (Објављена верзија)
,
© 2019
Метаподаци
Приказ свих података о документуАпстракт
The simple green method for synthesis of stable L-Methionine (L-Met) capped silver (Ag@LM NPs) and gold (Au@LM NPs) nanoparticles (NPs) without adding any additional reduction agent or stabilizer was developed. Colloidal dispersions were characterized by UV–Vis spectrophotometry. The size and spherical shape of NPs were evaluated by transmission electron microscopy. Their surface covering was confirmed by atomic force microscopy, Fourier transform infrared spectroscopy, dynamic light scattering, and zeta potential measurements. Density functional theory calculations pointed that the preferential adsorption mode of L-Met on both Ag and Au surfaces was a vertical binding geometry via –NH2 group, while horizontal binding mode via [sbnd]S[sbnd] and –NH2 groups is also possible. The genotoxicity (evaluated by the micronucleus assay) of NPs, as well as their effects on some oxidative stress parameters (catalase activity, malondialdehyde level), were assessed in vitro using human peripheral b...lood cells as a model system. The influence of NPs on the morphology of lymphocyte cells studied using atomic force microscopy revealed that the membrane of cells remained unaffected after the treatment with NPs. When considering the effects of NPs on catalase activity and malondialdehyde level, neither particle type promoted oxidative stress. However, the treatment of lymphocytes with Ag@LM NPs induced a concentration-dependent enhancement of the micronuclei incidence and suppression of the cell proliferation while Au@LM NPs promoted cell proliferation, with no significant effects on micronuclei formation. The Ag@LM NPs were more prone to induce DNA damage than Au@LM NPs, which makes the latter type more suitable for further studies in nano-medicine. © 2019
Кључне речи:
Silver / Gold / Green / Nanoparticles / L-methionine / ToxicityИзвор:
Journal of Inorganic Biochemistry, 2020, 204, 110958-Финансирање / пројекти:
- Истраживања интеракција ензима са токсичним и фармаколошки активним молекулима (RS-MESTD-Basic Research (BR or ON)-172023)
- Водонична енергија - развој нових материјала: електролитичко добијање водоника, водоничне горивне ћелије, изотопски ефекти (RS-MESTD-Basic Research (BR or ON)-172045)
DOI: 10.1016/j.jinorgbio.2019.110958
ISSN: 0162-0134
PubMed: 31841761
WoS: 000528628200012
Scopus: 2-s2.0-85076247598
Колекције
Институција/група
VinčaTY - JOUR AU - Laban, Bojana B. AU - Ralević, Uroš AU - Petrović, Sandra AU - Leskovac, Andreja AU - Vasić Anićijević, Dragana D. AU - Marković, Mirjana AU - Vasić, Vesna M. PY - 2020 UR - https://vinar.vin.bg.ac.rs/handle/123456789/8673 AB - The simple green method for synthesis of stable L-Methionine (L-Met) capped silver (Ag@LM NPs) and gold (Au@LM NPs) nanoparticles (NPs) without adding any additional reduction agent or stabilizer was developed. Colloidal dispersions were characterized by UV–Vis spectrophotometry. The size and spherical shape of NPs were evaluated by transmission electron microscopy. Their surface covering was confirmed by atomic force microscopy, Fourier transform infrared spectroscopy, dynamic light scattering, and zeta potential measurements. Density functional theory calculations pointed that the preferential adsorption mode of L-Met on both Ag and Au surfaces was a vertical binding geometry via –NH2 group, while horizontal binding mode via [sbnd]S[sbnd] and –NH2 groups is also possible. The genotoxicity (evaluated by the micronucleus assay) of NPs, as well as their effects on some oxidative stress parameters (catalase activity, malondialdehyde level), were assessed in vitro using human peripheral blood cells as a model system. The influence of NPs on the morphology of lymphocyte cells studied using atomic force microscopy revealed that the membrane of cells remained unaffected after the treatment with NPs. When considering the effects of NPs on catalase activity and malondialdehyde level, neither particle type promoted oxidative stress. However, the treatment of lymphocytes with Ag@LM NPs induced a concentration-dependent enhancement of the micronuclei incidence and suppression of the cell proliferation while Au@LM NPs promoted cell proliferation, with no significant effects on micronuclei formation. The Ag@LM NPs were more prone to induce DNA damage than Au@LM NPs, which makes the latter type more suitable for further studies in nano-medicine. © 2019 T2 - Journal of Inorganic Biochemistry T1 - Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles VL - 204 SP - 110958 DO - 10.1016/j.jinorgbio.2019.110958 ER -
@article{ author = "Laban, Bojana B. and Ralević, Uroš and Petrović, Sandra and Leskovac, Andreja and Vasić Anićijević, Dragana D. and Marković, Mirjana and Vasić, Vesna M.", year = "2020", abstract = "The simple green method for synthesis of stable L-Methionine (L-Met) capped silver (Ag@LM NPs) and gold (Au@LM NPs) nanoparticles (NPs) without adding any additional reduction agent or stabilizer was developed. Colloidal dispersions were characterized by UV–Vis spectrophotometry. The size and spherical shape of NPs were evaluated by transmission electron microscopy. Their surface covering was confirmed by atomic force microscopy, Fourier transform infrared spectroscopy, dynamic light scattering, and zeta potential measurements. Density functional theory calculations pointed that the preferential adsorption mode of L-Met on both Ag and Au surfaces was a vertical binding geometry via –NH2 group, while horizontal binding mode via [sbnd]S[sbnd] and –NH2 groups is also possible. The genotoxicity (evaluated by the micronucleus assay) of NPs, as well as their effects on some oxidative stress parameters (catalase activity, malondialdehyde level), were assessed in vitro using human peripheral blood cells as a model system. The influence of NPs on the morphology of lymphocyte cells studied using atomic force microscopy revealed that the membrane of cells remained unaffected after the treatment with NPs. When considering the effects of NPs on catalase activity and malondialdehyde level, neither particle type promoted oxidative stress. However, the treatment of lymphocytes with Ag@LM NPs induced a concentration-dependent enhancement of the micronuclei incidence and suppression of the cell proliferation while Au@LM NPs promoted cell proliferation, with no significant effects on micronuclei formation. The Ag@LM NPs were more prone to induce DNA damage than Au@LM NPs, which makes the latter type more suitable for further studies in nano-medicine. © 2019", journal = "Journal of Inorganic Biochemistry", title = "Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles", volume = "204", pages = "110958", doi = "10.1016/j.jinorgbio.2019.110958" }
Laban, B. B., Ralević, U., Petrović, S., Leskovac, A., Vasić Anićijević, D. D., Marković, M.,& Vasić, V. M.. (2020). Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles. in Journal of Inorganic Biochemistry, 204, 110958. https://doi.org/10.1016/j.jinorgbio.2019.110958
Laban BB, Ralević U, Petrović S, Leskovac A, Vasić Anićijević DD, Marković M, Vasić VM. Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles. in Journal of Inorganic Biochemistry. 2020;204:110958. doi:10.1016/j.jinorgbio.2019.110958 .
Laban, Bojana B., Ralević, Uroš, Petrović, Sandra, Leskovac, Andreja, Vasić Anićijević, Dragana D., Marković, Mirjana, Vasić, Vesna M., "Green synthesis and characterization of nontoxic L-methionine capped silver and gold nanoparticles" in Journal of Inorganic Biochemistry, 204 (2020):110958, https://doi.org/10.1016/j.jinorgbio.2019.110958 . .