Estradiol‐mediated regulation of hepatic iNOS in obese rats: Impact of Src, ERK1/2, AMPKα, and miR‐221
Nema prikaza
Autori
Panić, AnastasijaStanimirović, Julijana
Obradović, Milan M.
Sudar-Milovanović, Emina
Perović, Milan
Lačković, Milena
Petrović, Nina
Isenović, Esma R.
Članak u časopisu (Objavljena verzija)
,
© 2018 International Union of Biochemistry and Molecular Biology, Inc.
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Purpose: This study aimed to investigate in vivo effects of estradiol on the regulation of hepatic inducible nitric oxide synthase (iNOS) expression in the high fat (HF) diet-induced obesity. Also, we aimed to investigate whether activation of the extracellular signal-regulated kinase (ERK1/2), adenosine monophosphate-activated protein kinase (AMPK), Src kinase, and miR-221 is involved in estradiol-mediated regulation of iNOS in the liver of obese male Wistar rats. Male Wistar rats were fed a standard laboratory diet or a HF diet for 10 weeks. Half of HF rats were treated with estradiol intraperitoneally (40 μg/kg), whereas the other half were placebo-treated 24 H before euthanasia. Results show that estradiol treatment of HF rats decreased hepatic iNOS mRNA (P < 0.05) and protein expression (P < 0.01), the protein levels of p65 subunit of nuclear factor κB (P < 0.05) and ERα (P < 0.05), ERK1/2 phosphorylation (P < 0.001), and ERα/Src kinase association (P < 0.05). By contrast, hepatic... Src protein level (P < 0.05), AMPKα phosphorylation (P < 0.05), and miR-221 expression (P < 0.05) were increased in HF rats after estradiol treatment. Our results indicate that estradiol in vivo regulates hepatic iNOS expression in obese rats via molecular mechanisms involving ERK1/2, AMPK, Src, and miR-221 signaling. © 2018 International Union of Biochemistry and Molecular Biology, Inc.
Ključne reči:
estradiol / HF diet / iNOS / liver / miR-221Izvor:
Biotechnology and Applied Biochemistry, 2018, 65, 6, 797-806Finansiranje / projekti:
- Hormonska regulacija ekspresije i aktivnosti azot oksid sintaze i natrijum-kalijumove pumpe u eksperimentalnim modelima insulinske rezistencije, dijabetesa i kardiovaskularnih poremećaja (RS-MESTD-Basic Research (BR or ON)-173033)
- Efekti modulacije biohumoralnog, inflamatornog i metaboličkog odgovora u akutnom infarktu miokarda sa elevacijom ST-segmenta na ishod lečenja i srčanu funkciju (RS-MESTD-Basic Research (BR or ON)-175099)
DOI: 10.1002/bab.1680
ISSN: 0885-4513; 1470-8744
PubMed: 29957877
WoS: 000454576300004
Scopus: 2-s2.0-85052436131
Kolekcije
Institucija/grupa
VinčaTY - JOUR AU - Panić, Anastasija AU - Stanimirović, Julijana AU - Obradović, Milan M. AU - Sudar-Milovanović, Emina AU - Perović, Milan AU - Lačković, Milena AU - Petrović, Nina AU - Isenović, Esma R. PY - 2018 UR - https://vinar.vin.bg.ac.rs/handle/123456789/8399 AB - Purpose: This study aimed to investigate in vivo effects of estradiol on the regulation of hepatic inducible nitric oxide synthase (iNOS) expression in the high fat (HF) diet-induced obesity. Also, we aimed to investigate whether activation of the extracellular signal-regulated kinase (ERK1/2), adenosine monophosphate-activated protein kinase (AMPK), Src kinase, and miR-221 is involved in estradiol-mediated regulation of iNOS in the liver of obese male Wistar rats. Male Wistar rats were fed a standard laboratory diet or a HF diet for 10 weeks. Half of HF rats were treated with estradiol intraperitoneally (40 μg/kg), whereas the other half were placebo-treated 24 H before euthanasia. Results show that estradiol treatment of HF rats decreased hepatic iNOS mRNA (P < 0.05) and protein expression (P < 0.01), the protein levels of p65 subunit of nuclear factor κB (P < 0.05) and ERα (P < 0.05), ERK1/2 phosphorylation (P < 0.001), and ERα/Src kinase association (P < 0.05). By contrast, hepatic Src protein level (P < 0.05), AMPKα phosphorylation (P < 0.05), and miR-221 expression (P < 0.05) were increased in HF rats after estradiol treatment. Our results indicate that estradiol in vivo regulates hepatic iNOS expression in obese rats via molecular mechanisms involving ERK1/2, AMPK, Src, and miR-221 signaling. © 2018 International Union of Biochemistry and Molecular Biology, Inc. T2 - Biotechnology and Applied Biochemistry T1 - Estradiol‐mediated regulation of hepatic iNOS in obese rats: Impact of Src, ERK1/2, AMPKα, and miR‐221 VL - 65 IS - 6 SP - 797 EP - 806 DO - 10.1002/bab.1680 ER -
@article{ author = "Panić, Anastasija and Stanimirović, Julijana and Obradović, Milan M. and Sudar-Milovanović, Emina and Perović, Milan and Lačković, Milena and Petrović, Nina and Isenović, Esma R.", year = "2018", abstract = "Purpose: This study aimed to investigate in vivo effects of estradiol on the regulation of hepatic inducible nitric oxide synthase (iNOS) expression in the high fat (HF) diet-induced obesity. Also, we aimed to investigate whether activation of the extracellular signal-regulated kinase (ERK1/2), adenosine monophosphate-activated protein kinase (AMPK), Src kinase, and miR-221 is involved in estradiol-mediated regulation of iNOS in the liver of obese male Wistar rats. Male Wistar rats were fed a standard laboratory diet or a HF diet for 10 weeks. Half of HF rats were treated with estradiol intraperitoneally (40 μg/kg), whereas the other half were placebo-treated 24 H before euthanasia. Results show that estradiol treatment of HF rats decreased hepatic iNOS mRNA (P < 0.05) and protein expression (P < 0.01), the protein levels of p65 subunit of nuclear factor κB (P < 0.05) and ERα (P < 0.05), ERK1/2 phosphorylation (P < 0.001), and ERα/Src kinase association (P < 0.05). By contrast, hepatic Src protein level (P < 0.05), AMPKα phosphorylation (P < 0.05), and miR-221 expression (P < 0.05) were increased in HF rats after estradiol treatment. Our results indicate that estradiol in vivo regulates hepatic iNOS expression in obese rats via molecular mechanisms involving ERK1/2, AMPK, Src, and miR-221 signaling. © 2018 International Union of Biochemistry and Molecular Biology, Inc.", journal = "Biotechnology and Applied Biochemistry", title = "Estradiol‐mediated regulation of hepatic iNOS in obese rats: Impact of Src, ERK1/2, AMPKα, and miR‐221", volume = "65", number = "6", pages = "797-806", doi = "10.1002/bab.1680" }
Panić, A., Stanimirović, J., Obradović, M. M., Sudar-Milovanović, E., Perović, M., Lačković, M., Petrović, N.,& Isenović, E. R.. (2018). Estradiol‐mediated regulation of hepatic iNOS in obese rats: Impact of Src, ERK1/2, AMPKα, and miR‐221. in Biotechnology and Applied Biochemistry, 65(6), 797-806. https://doi.org/10.1002/bab.1680
Panić A, Stanimirović J, Obradović MM, Sudar-Milovanović E, Perović M, Lačković M, Petrović N, Isenović ER. Estradiol‐mediated regulation of hepatic iNOS in obese rats: Impact of Src, ERK1/2, AMPKα, and miR‐221. in Biotechnology and Applied Biochemistry. 2018;65(6):797-806. doi:10.1002/bab.1680 .
Panić, Anastasija, Stanimirović, Julijana, Obradović, Milan M., Sudar-Milovanović, Emina, Perović, Milan, Lačković, Milena, Petrović, Nina, Isenović, Esma R., "Estradiol‐mediated regulation of hepatic iNOS in obese rats: Impact of Src, ERK1/2, AMPKα, and miR‐221" in Biotechnology and Applied Biochemistry, 65, no. 6 (2018):797-806, https://doi.org/10.1002/bab.1680 . .